13064results about "Animal feeding stuff" patented technology

Provided is a Limosilactobacillus fermentum VB216, which has the deposit number of CGMCC No. 26578 in the China General Microbiological Culture Collection Center, and has the deposit date of 20 February 2023. The Limosilactobacillus fermentum has inhibitory activities with regard to a variety of pathogenic bacteria and excellent activity for degrading arginine. The present invention can be used in the preparation of products, such as food, health-care food, medicine and feed, for preventing and / or relieving and / or treating arginine metabolism-related diseases, such as hyperarginemia, malignant tumor treatment, allergic reactions, eczema, pruritus and abdominal spasm, and has great application prospects.

The invention relates to the technical field of animal feed preparation, in particular to a functional feed additive for ruminants and a preparation method of the functional feed additive for the ruminants, and the functional feed additive for the ruminants is characterized by consisting of the following components in percentage by mass: probiotics which are saccharomycetes, bacillus subtilis and enterococcus faecalis; an enzyme preparation, xylanase and protease; prebiotics are added; a microcapsule coating material; a binder; the balance is a carrier; the probiotics and the enzyme preparation are wrapped in the chitosan / sodium alginate composite film through a microcapsule coating technology, adverse factors such as temperature, humidity and illumination in the production, transportation and storage process can be effectively isolated, meanwhile, in the rumen low-pH, strong-protease and complex-microorganism environment, the coating layer can slowly release and protect active ingredients, and the effect of preventing and treating the probiotics and the enzyme preparation is achieved. Effective content and activity during final feeding are ensured, consistent growth promotion can be kept in different varieties, weights and feeding stages, and production benefits and feeding stability are improved.

The invention belongs to the technical field of biology, and discloses a Tibetan pig source bacillus subtilis, the classification name is bacillus subtilis Yirun2024, the Latin name is bacillus subtilis Yirun2024, the preservation number is CCTCC NO: M 20241752, the preservation date is August 7, 2024, the preservation unit is China Center for Type Culture Collection, and the preservation address is Wuhan University, Wuhan, China. When the bacillus subtilis is fermented for 12 hours in a shake flask, the endo-cellulase activity, the exo-cellulase activity and the beta-glucosidase activity of the bacillus subtilis are respectively up to 2902.72 U / mL, 11026.33 U / mL and 2983.04 U / mL, which are far better than those of similar engineering bacteria. Meanwhile, the invention further provides application of the bacillus subtilis, the bacillus subtilis has important significance in the field of promotion of application of fiber resources, and meanwhile, the bacillus subtilis can keep a high survival rate in a high-temperature environment.

A surround hot air slow drying device and a three-stage baking method for pet food processing are provided. The surround hot air slow drying device for pet food processing includes a drying box. A hot air drying unit is provided at an inner bottom of the drying box, a circulating conveying unit is provided inside the drying box, a plurality of drying frames are evenly distributed on the circulating conveying unit, a material turning mechanism is provided inside the drying frames, and a positioning component is provided inside the drying box. The material turning mechanism cooperates with the positioning component to turn the raw materials in the drying frames.

The invention discloses lactococcus gasseri C6a2 and application thereof. The preservation number of the lactococcus gasseri C6a2 is CGMCC (China General Microbiological Culture Collection Center) NO: 31024; the 16S rRNA (ribosomal Ribonucleic Acid) gene sequence of the lactococcus gasseri C6a2 is as shown in SEQ ID NO: 1. The bacterial strain is a macrobrachium rosenbergii intestinal absolute dominant bacterium, has a typical probiotic function, is high in growth speed and activity, can quickly enter a logarithmic phase, has relatively high acid production and acid resistance, can secrete lipase, has relatively high bacteriostatic activity on staphylococcus aureus, aeromonas hydrophila and aeromonas veronii, and can be used for preparing the macrobrachium rosenbergii. The bacterium can be used for preparing a special probiotic preparation for macrobrachium rosenbergii, so that the sustainable development of the macrobrachium rosenbergii industry in China is promoted.

The invention provides a traditional Chinese medicine composition and an application thereof in preparing a product for preventing, alleviating or treating acute hepatopancreatic necrosis of shrimp, belonging to the field of veterinary medicine. The active ingredients of the traditional Chinese medicine composition include isatis root, artificial bezoar, liquorice, borneol, pig gall powder and xuanming powder. The traditional Chinese medicine composition has a good therapeutic effect on preventing, alleviating or treating acute hepatopancreatic necrosis of shrimp, with a cure rate of more than 70%, has antibacterial and liver-protecting effects, can effectively improve the degree of liver damage, improve the survival rate of shrimp and reduce losses.

The invention belongs to the technical field of microbial fermentation and food engineering, and discloses a fermentation method for enhancing the production capacity of schizochytrium limacinum phospholipid DHA and application, schizochytrium limacinum is subjected to fermentation culture for 32-60 h, and the addition of an organic nitrogen source and phosphate is optimized in the fermentation culture process; the formula of a seed culture medium for culturing schizochytrium limacinum is not changed, and only the formula of a fermentation culture medium for schizochytrium limacinum is changed. The invention provides a fermentation strategy for enhancing the production capacity of the schizochytrium limacinum phospholipid DHA, and the schizochytrium limacinum can obtain the phospholipid DHA with higher content by optimizing an organic nitrogen source and phosphate in a schizochytrium limacinum fermentation culture medium. According to the obtained schizochytrium limacinum grease, the total DHA content is obviously increased compared with that of a traditional fermentation strategy, and the proportion of phospholipid DHA is also remarkably increased. The regulation and control method is applied to preparation of schizochytrium limacinum algae powder, the schizochytrium limacinum algae powder rich in phospholipid DHA is obtained, and application of the schizochytrium limacinum algae powder in the field of aquatic feed is facilitated.

The invention provides an ecology-imitating artificial propagation method for Dongquasichthys nobilis. The ecology-imitating artificial propagation method comprises the following steps: S1, selecting male and female parent fishes and putting the male and female parent fishes into an artificial ecology-imitating breeding pond for breeding; s2, in a non-breeding season, feeding female parent fish feed A for 2-3 times every day, and feeding male parent fish feed B for 2-3 times every day; s3, 2-3 months before breeding, female parent fish fortified feed I is fed 2-3 times every day, and male parent fish fortified feed II is fed 2-3 times every day; s4, after the gonad of the parent fish is mature, artificial spawning induction is conducted, and fertilized eggs are collected and hatched. According to the method, the ecology-imitating breeding environment is manufactured, so that the east astrostroma parent fish adapts to the artificial environment, the irritability is reduced, the east astrostroma parent fish is fed with different feeds in the non-breeding season and the breeding season, the ecological factor and nutritional requirements for gonad development of the east astrostroma can be met, the immunity of the east astrostroma parent fish is improved, gonad maturation is promoted, and the survival rate of the east astrostroma parent fish is increased. The quality of sperms and ova is improved, the fertilization probability is improved, the malformation probability of hatched larvae is reduced, and the breeding efficiency is improved.

The invention relates to the technical field of feed additives, and discloses a feed additive and application thereof in yak breeding. The feed additive comprises the following raw materials in parts by mass: 10-20 parts of mixed bean flour, 5-10 parts of a traditional Chinese medicine extract, 3-5 parts of lactic acid bacteria and 1-3 parts of an acid preparation. The feed additive provided by the invention significantly inhibits the expression of foot and mouth disease virus mRNA and inhibits the replication of foot and mouth disease virus; when the feed additive provided by the invention is added into daily feed for yaks, the foot-and-mouth disease of yaks can be effectively prevented and treated.

The invention discloses an amino acid phagostimulant for juvenile helichthys nobilis, which mainly comprises aspartic acid, glutamic acid, alanine, glycine, proline and methionine in a content ratio of (4-5): (5-6): (4-5): 3: 2: 1, and the six amino acids are reasonably compounded, so that the phagostimulant for the juvenile helichthys nobilis achieves the best phagostimulant effect on the juvenile helichthys nobilis. The invention also discloses a juvenile mollusca lanuginosa feed based on the amino acid phagostimulant, the juvenile mollusca lanuginosa feed comprises a basal feed and the phagostimulant, and the mass percentage content of the phagostimulant in the juvenile mollusca lanuginosa feed is 0.79-0.96%. The amino acid phagostimulant is added into the basal feed, so that the food-calling fresh fruit of the young mollusca is obviously higher than that of traditional baits, the increase rate of the body weight and the body length of the young mollusca is higher, the survival rate is maximum, and the application prospect is very good.

The invention relates to the technical field of pet health care, in particular to a probiotic composite additive for pet intestinal health and a preparation method of the probiotic composite additive. Comprising the following basic components in percentage by mass: 40% of lactobacillus complex, 10% of bifidobacterium complex, 10% of saccharomyces boulardii, 10% of prebiotic complex, 10% of natural digestive enzyme, 10% of functional plant extract, 10% of carrier and the like. 30% of a bifidobacterium complex group; 15% of saccharomyces boulardii; 8% of a prebiotic compound; 5% of natural digestive enzyme; and 2% of a functional plant extract. The multiple probiotics have a synergistic effect, the effect is better, the prebiotics promote proliferation of the probiotics and can enhance the effect of the additive, meanwhile, through drying treatment, the product is good in stability, long in shelf life and small in package design, the additive can be conveniently added to pet food, intestinal health of pets can be effectively improved, and the preparation method is simple and suitable for large-scale production.

The invention relates to the technical field of microorganisms, in particular to application of streptococcus non-lactolyticus GX-7 and a complex microbial inoculant thereof in sugarcane leaf silage, the strain GX-7 is separated from healthy pig manure by a research group, and through detection, the strain has good cholate resistance and is suitable for surviving in intestinal tracts, and the strain GX-7 has good cholate resistance and is suitable for being used as a feed additive. The antibacterial effect on pseudomonas fluorescens, listeria monocytogenes, salmonella choleraesuis and shigella flexneri is achieved; the strain has a good fermentation capability on sugarcane leaves, and the sugarcane leaves are inoculated with a complex microbial inoculant prepared from streptococcus non-lactolyticus GX-7 and streptococcus non-lactolyticus GX-2, so that a synergistic fermentation effect can be achieved; after the silage prepared from the complex microbial inoculant is fed to female buffalo in the gestation period, it is found that the silage can effectively increase the content of milk protein, milk total solids and milk non-fat solids in colostrum of the female buffalo and relieve diarrhea of the female buffalo, and the silage is safe and good.

The invention belongs to the field of molecular biology, and particularly relates to a transgenic soybean event LP207-1 and a detection method thereof. The invention provides a nucleic acid sequence for detecting a transgenic soybean event LP207-1, the nucleic acid sequence is selected from one or more of sequences SEQ ID NO: 1-7 or complementary sequences thereof, and the nucleic acid sequence is derived from a plant, a seed or a cell of the transgenic soybean event LP207-1. A representative sample of a seed of the transgenic soybean event LP207-1 is preserved in the China Center for Type Culture Collection on May 23, 2025, and the preservation number is CCTCC NO: P202516. The transgenic soybean event LP207-1 has good resistance to lepidoptera pests and can tolerate agricultural herbicides containing glyphosate and glufosinate-ammonium, economic loss caused by the lepidoptera pests can be avoided, and the yield is not reduced; and the breeding efficiency is enhanced, and the molecular marker can be used for tracking a breeding population and transgenic insertion fragments in offspring of the breeding population. The detection method provided by the invention can quickly, accurately and stably identify the existence of the plant material derived from the transgenic soybean event LP207-1.

The invention discloses a ruminant feed capable of replacing soybean meal as well as a preparation method and application of the ruminant feed. The ruminant animal feed is prepared from the following raw material matrixes in parts by weight through compound probiotic fermentation: 45-55 parts of corn sugar residues, 15-25 parts of corn steep liquor, 10-20 parts of corn germ meal, 3-8 parts of extruded soybeans, 3-8 parts of corn protein powder, 2-4 parts of urea and 0.5-1.5 parts of lysine. The compound probiotics are prepared from saccharomyces cerevisiae, lactobacillus plantarum and bacillus subtilis. The invention further discloses a preparation method of the ruminant feed and application of the ruminant feed in completely replacing soybean meal in daily ration of ruminants. The ruminant feed disclosed by the invention can completely replace soybean meal in daily ration of ruminants, and even if the ruminants are in a fattening period, the soybean meal does not need to be additionally added to maintain protein supply, so that the feed cost is effectively reduced, and multiple targets of saving cost, improving efficiency and protecting environment are achieved.

The invention discloses a traditional Chinese medicine residue treatment method and application of a fermented traditional Chinese medicine residue preparation prepared through the traditional Chinese medicine residue treatment method in feed, and belongs to the technical field of waste resource recycling. The lactobacillus plantarum is used for fermenting the traditional Chinese medicine residues, and the prepared fermented traditional Chinese medicine residue preparation improves the content of phenolic substances and the activity of flavonoid substances; finally, the fermented traditional Chinese medicine residue preparation prepared by the treatment method of the traditional Chinese medicine residues is applied to preparation of feed, so that the growth performance of the Rongchang pigs can be improved, the diarrhea rate can be reduced, the immune function can be enhanced, resource utilization and reduction treatment of the traditional Chinese medicine residues can be realized, and a high-quality feed additive can be provided for livestock and poultry breeding; the sustainable development of animal husbandry is promoted.

The invention belongs to the technical field of microorganisms, and particularly relates to a postbiotic preparation for pets as well as a preparation method and application of the postbiotic preparation. The metabolite preparation provided by the invention comprises a lactobacillus plantarum Ps-8 inactivated thallus and a metabolite thereof, a lactobacillus casei C-9 inactivated thallus and a metabolite thereof, a bifidobacterium animalis subsp. Lactis BB-16 inactivated thallus and a metabolite thereof, and a lactobacillus acidophilus LA-14 inactivated thallus and a metabolite thereof. The pet food can be applied to various food types of different varieties of cat and dog pets, and has the effects of improving the intestinal barrier function of the pets, enhancing the immunity, reducing the incidence rate of diarrhea, reducing the food change stress, promoting the digestion of nutrient substances, optimizing the state of spirit and fur, reducing the odor of excrement, fading the odor of the oral cavity, improving the health level of the oral cavity and teeth and the like.

Provided is a Bifidobacterium lactis. The Bifidobacterium lactis is Bifidobacterium animalis subsp. lactis BL-11, and the preservation number of the Bifidobacterium animalis subsp. lactis BL-11 is CGMCC No. 20847. The provided Bifidobacterium animalis subsp. Lactis can improve intestine metabolism disorder and normalize the composition of intestine microbiome so as to improve height, suppresses obesity, promote infant and youth physical development and mental development, and prevent and treat mental disorders, said mental disorders including anxiety, depression, attention-deficit / hyperactivity disorder, autism, schizophrenia, hepatic encephalopathy, anorexia, Tourette syndrome, and Asperger syndrome.

The invention discloses bacillus subtilis and application thereof in rapeseed meal fermentation. The bacillus subtilis is named as bacillus subtilis H8, and the preservation number of the bacillus subtilis is CCTCC (China Center For Type Culture Collection) NO: M20241745; the 16S rDNA gene sequence of the bacillus subtilis H8 is as shown in SEQ ID No: 1. The strain can effectively degrade the content of antinutritional factors in the rapeseed dregs, and the nutritional characteristics of the rapeseed dregs are improved through solid fermentation of the rapeseed dregs. According to the method, the problem that the fermentation effect of existing feed fermentation raw materials is not ideal is solved, and through multidisciplinary cross research and industrial application and popularization, the rapeseed meal fermentation technology provides important support for resource recycling and agricultural sustainable development.

The invention provides a breeding method of golden and tiger hybrid spotted fish eggs. The breeding method comprises the following steps: S1, parent screening; s2, parent cultivation; s3, artificial insemination: (1) spawning induction: when the belly of the female parent fish expands, the ovary develops to the IV stage and the male parent fish presses the belly to cause white seminal fluid to flow out, performing oxygenation flowing water stimulation on the female parent fish, and performing irradiation with a TDP radiation lamp for spawning induction; (2) fertilization; s4, artificial incubation: (1) arranging an incubator, injecting seawater, and performing plasma treatment to obtain a treated incubator; and (2) adding the fertilized eggs into the treated incubator, carrying out magnetization treatment, adding chitosan, and hatching to obtain the larva fish. According to the breeding method, the sperm and egg quality can be improved, the fertilization rate and the hatching rate are remarkably increased, and according to verification of the embodiment, the fertilization rate ranges from 97.4% to 99.3%, and the hatching rate ranges from 95.6% to 97.1%. The hatched larvae are not deformed, the survival rate is 96%-99%, the quality of the larvae is high, and a good foundation is established for obtaining high-quality hybrid Jinhuasu.

The invention discloses a lytic bacteriophage vBVcaPR25D and an application of the lytic bacteriophage vBVcaPR25D. The collection number of the lytic bacteriophage vBVcaPR25D is GDMCC (Graphical Digital Microbial Culture Collection Center) No: 65167-B1. The lytic bacteriophage vBVcaPR25D provided by the invention has a high specificity and a remarkable killing effect on vibrio cantoniensis V.campbelli LMG 11216T, and can be used for killing vibrio cantoniensis V.campbelli LMG 11216T. Besides, virulence factor genes and drug-resistant genes are not detected in the genome of the bacteriophage, so that the bacteriophage is high in safety and environment-friendly, and can be used as a bactericide for aquatic vibrio candersonii diseases.

The invention discloses a virulent bacteriophage vBValSR36H with a host being vibrio alginolyticus and application of the virulent bacteriophage vBValSR36H, and relates to the technical field of microbial control. The invention provides a bacteriophage capable of efficiently cracking Vibrio alginolyticus ATCC 17749T, the bacteriophage is named as vBValSR36H, the preservation number is 66248-B1, and the preservation address is Institute of Microbiology, Academy of Sciences in Guangdong Province. The vibrio alginolyticus bacteriophage vBValSR36H provided by the invention has good environmental stability and certain bactericidal activity, and has a relatively wide environmental adaptation range and relatively long survival time. The bacteriophage vBValSR36H provided by the invention can specifically and effectively kill, prevent and control related vibrio diseases, meanwhile, virulence factor genes and drug-resistant genes are not detected in the bacteriophage, and the bacteriophage vBValSR36H has the advantages of no toxic or side effect, high safety, environmental friendliness and wide application prospect. Therefore, the vibrio alginolyticus bacteriophage vBValSR36H can be completely applied to killing and / or preventing vibrio alginolyticus for non-disease diagnosis and treatment purposes.

The invention relates to the field of microbial fermented feed, in particular to bamboo powder fermented feed rich in amino acid and a preparation method thereof. According to the preparation method of the bamboo powder fermented feed provided by the invention, bamboo is used as a base material for fermentation and enzymolysis to prepare amino acid, and then some conventional raw material components of the feed are added to prepare the bamboo powder fermented feed rich in amino acid. The digestibility and the nutrition utilization rate of animals on the feed are higher, the oxidation resistance is stronger, and the palatability is better.

The invention relates to the technical field of flavor agents, and provides a freshwater fish flavor pet flavor agent and a production method thereof, and the freshwater fish flavor pet flavor agent comprises the following components: freshwater fish, an enzyme preparation, reducing sugar, vitamins, amino acids, auxiliary materials and a herbaceous plant aqueous extract. Through scientific raw material selection, enzymic preparation proportion control, multi-element reducing sugar matching, comprehensive vitamin combination, balanced amino acid screening, herbaceous plant aqueous extract addition, nanotechnology wrapping operation and careful adjustment of auxiliary materials, the pet flavoring agent has the effects of special fragrance, rich taste, good palatability, immunity enhancement and the like.

The invention discloses a microbial compound feed additive, which is prepared from the following ingredients in parts by weight: 35 to 45 parts of traditional Chinese medicine residues, 25 to 35 parts of soybean meal, 15 to 25 parts of bran, 8 to 12 parts of corn flour, 0.3 to 0.7 part of selenium-enriched yeast, 8 to 12 parts of aspergillus niger spore powder, 10 to 15 parts of bacillus subtilis, 6 to 9 parts of lactobacillus plantarum and 5 to 8 parts of candida utilis. The invention relates to the technical field of feed additives, through the synergistic effect of the aspergillus niger, the bacillus subtilis, the lactobacillus plantarum and the candida utilis, the aspergillus niger can generate various enzymes, and decomposition of raw materials and release of nutritional ingredients are facilitated; the bacillus subtilis can enhance the immunity of livestock and poultry and inhibit the growth of harmful bacteria; lactobacillus plantarum can regulate intestinal microecological balance and promote digestion and absorption; candida utilis is rich in protein and vitamins and provides nutrition for livestock and poultry, the four components cooperate with each other, the function limitation of a single strain is broken through, and the health level and production performance of livestock and poultry are comprehensively improved.

The invention discloses a broad-spectrum lytic bacteriophage for preventing and controlling various vibrio infections in an aquaculture environment. The invention relates to V.alginolyticus phage vBValM-R37J, which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on August 6, 2025, the address is No.100 Courtyard, Xianlie Middle Road, Xianlie District, Guangzhou City, Guangdong Province, the postcode is 510070, and the preservation number is GDMCC NO.66813-B1. The invention further discloses a method for preparing the V.alginolyticus phage vBValM-R37J strain. The bacteriophage R37J is a candidate therapeutic bacteriophage with wide host spectrum, high splitting efficiency, gene safety and environmental stability, and has the potential of being popularized and applied in aquaculture as a novel biological tool for accurately preventing and controlling various vibrio infections. A new biological resource is provided for accurate prevention and control of aquatic pathogenic bacteria, and a theoretical basis and a technical support are provided for popularization and application of a phage therapy in an actual culture system.

The invention discloses a feed functional compound for improving salt-alkali stress resistance of macrobrachium rosenbergii and application of the feed functional compound, and belongs to the technical field of aquaculture. The feed functional compound for improving saline-alkaline stress resistance of macrobrachium rosenbergii provided by the invention comprises vitamin D3, betaine and sodium oleate. According to the invention, the absorption promotion effect of vitamin D3 on calcium and phosphorus ions is utilized, so that the ion metabolism disorder of macrobrachium rosenbergii under saline-alkali stress is improved; by utilizing the osmotic pressure regulation capability of betaine, osmotic pressure unbalance under saline-alkali stress is improved, and the energy consumption of osmotic regulation is reduced; the sodium oleate is used for improving energy supply, the membrane structure is adjusted to improve osmotic pressure adjusting capacity, and the three components act together to achieve the purpose of improving salt-alkali stress tolerance of macrobrachium rosenbergii.

The invention discloses a strain of desert algae improved by space breeding and application of the desert algae. According to the invention, a strain of desert algae is obtained through variation induced by a space environment and ground screening, and the strain of desert algae shows high growth rate and excellent outdoor adaptability, and can efficiently accumulate two bioactive substances, namely algal polysaccharides and flavones, which have important commercial and health values. Therefore, the strain of the desert algae shows better application potential in the aspects of producing high-quality desert algae powder, polysaccharide, flavone and the like.

The invention discloses a lytic bacteriophage vBVcaPR24D and an application of the lytic bacteriophage vBVcaPR24D. The collection number of the lytic bacteriophage vBVcaPR24D is GDMCC (Graphical Digital Microbial Culture Collection Center) No: 65166-B1. The lytic bacteriophage vBVcaPR24D provided by the invention has a high specificity and a remarkable killing effect on vibrio cantoniensis V.campbelli LMG 11216T, and can be used for killing vibrio cantoniensis V.campbelli LMG 11216T. Besides, virulence factor genes and drug-resistant genes are not detected in the genome of the bacteriophage, so that the bacteriophage is high in safety and environment-friendly, and can be used as a bactericide for aquatic vibrio candersonii diseases.

The invention provides copper-loaded duck-origin lactobacillus plantarum with high antibacterial peptide yield and application of the copper-loaded duck-origin lactobacillus plantarum. The preservation number of the provided copper-loaded duck-origin lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) NO.32835. The domesticated and bred duck-origin lactobacillus plantarum with high antibacterial peptide yield can tolerate high-concentration copper ions, the domesticated and bred lactobacillus plantarum can stably grow, and the contents of antibacterial peptide, extracellular protein, lipase, amylase, lactic acid, acetic acid and the like in the domesticated and bred lactobacillus plantarum strain are increased; and the amino acid composition is changed. The strain can convert inorganic copper into organic copper, the utilization rate of copper is increased, and growth and development of organisms are promoted. Moreover, small peptides and extracellular proteins in metabiotics generated after fermentation have the effect of effectively inhibiting harmful bacteria such as riemerella anatipestifer, escherichia coli, salmonella and staphylococcus of poultry, and are beneficial to eliminating intestinal inflammatory factors, maintaining intestinal health and improving the feed utilization rate.

The invention relates to the technical field of microorganisms, in particular to nocardia seriolae bacteriophage as well as a composition and application thereof. The name of the nocardia seriolae bacteriophage is RDP-NS002, the nocardia seriolae bacteriophage is preserved in the China General Microbiological Culture Collection Center on August 25, 2025, and the preservation number of the nocardia seriolae bacteriophage is CGMCC No.46674. The nocardia seriolae bacteriophage is named as RDP-NS002, and the nocardia seriolae bacteriophage is preserved in the China General Microbiological Culture Collection Center on August 25, 2025. The composition comprises the bacteriophage. The bacteriophage or the composition is prepared in the following steps: (1) preparing a product for killing nocardia seriolae; (2) preparing a product for inhibiting nocardia seriola; and (3) application in preparation of a product for preventing and / or treating fish nocardia disease caused by nocardia seriolae. The phage has the characteristics of high thermal stability, high acid-base stability and high splitting rate, has a long-acting inhibition effect, and is beneficial to prevention and treatment of nocardia seriola diseases.