165results about How to "Reduce expression" patented technology

The invention relates to gardenia polysaccharide as well as a preparation method and application thereof. The gardenia polysaccharide comprises the following monosaccharide units: rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose, or mainly comprises rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose; the molar ratio of rhamnose to glucuronic acid to galacturonic acid to glucose to galactose to arabinose in the gardenia polysaccharide is (3-15): (1-8): (4-18): (1-6): (15-35): (25-70). According to the gardenia polysaccharide, FFA induced mouse primary parenchymal hepatic cell lipid accumulation can be reduced, no obvious cytotoxic effect exists, CDAHFD diet induced hepatic fibrosis can be reduced, and the gardenia polysaccharide has a good effect of preventing and / or treating non-alcoholic steatohepatitis.

The invention provides an application of a recombinant XVII type humanized collagen or a composition containing the recombinant XVII type humanized collagen in preparation of a preparation for preventing and / or treating lupus erythematosus, which can overcome the defect that more adverse reactions are generated in treatment in the existing scheme.

The invention discloses application of a Wnt2 gene in buffalo ovarian granular cells, and belongs to the technical field of cell engineering and gene engineering. Wnt2 is taken as a research object, the Wnt2 overexpression vector or Wnt2 siRNA is transfected to the ovarian granulosa cells respectively by constructing the Wnt2 overexpression vector and synthesizing small interfering RNA (Wnt2 siRNA), then changes of signal channel gene mRNA and protein level related to ovarian granulosa cell apoptosis, proliferation and E2 and P4 secretion are detected, and finally phenotypic changes of the ovarian granulosa cells are detected. Results show that Wnt2 can inhibit apoptosis of ovarian granular cells and promote proliferation and E2 and P4 secretion. By exploring the application of the Wnt2 in the ovarian granular cells, the application has good application in researching an ovarian follicle atresia mechanism, initial estrus initiation and the like.

The invention discloses a precipitation-supported slow-release carbon-based iron fertilizer and a preparation method thereof, and the preparation method specifically comprises the following steps: firstly, putting a biological carapace heavy metal adsorbent into a calcium hydroxide solution for dipping, drying, then putting the biological carapace heavy metal adsorbent into a ferrous sulfate solution for dipping in two times, and finally drying and granulating. Precipitated ferric hydroxide, a ferrous hydroxide mixture and excessive ferric sulfate are uniformly dispersed and attached to the interiors of pore channels of the porous adsorbent, and the excessive ferric sulfate can quickly provide iron ions for antagonistic cadmium and arsenic ion absorption in the early growth stage of rice. Precipitated ferric hydroxide and ferrous hydroxide wrapped by calcium sulfate in the porous adsorbent not only can realize long-acting slow release of iron ions, but also can reduce the oxygen content of soil, and iron ion antagonism and precipitation type adsorption loading modes are carried out in the whole growth process of rice, so that the release period of the iron ions is greatly prolonged, and the soil quality is improved. The iron fertilizer disclosed by the invention has an excellent rice cadmium-arsenic antagonism effect and has an excellent application prospect.

The invention belongs to the technical field of gene engineering and biology, discloses a modified adeno-associated virus AAV-8 serotype for retina wide targeting and expression, and further relates to construction and application of the modified type. 10 amino acid sequences as shown in SEQ ID NO.3 are inserted between the 590th amino acid and the 591th amino acid of an AAV-8 serotype capsid protein sequence as shown in SEQ ID NO.2, the first, second and tenth amino acids are protected amino acids, and the third to ninth amino acids are amino acid sequences obtained by screening. The modified serotype disclosed by the invention is stronger in fluorescence effect after infection and can be obviously and intuitively observed, an exogenous gene can be widely and efficiently expressed in a specific targeted tissue retina whole region, and the expression efficiency is greatly improved under the condition of using the same virus amount. And compared with a wild serotype, the serotype has better and wider targeted tissue expression and less non-targeted tissue expression.

The invention provides application of a P8 gene, or a P8 gene coding protein, or a recombinant vector or host bacteria containing a P8 gene coding region in improvement of GFP (Green Fluorescent Protein) expression quantity and / or inhibition of degradation of an mRNA (Messenger Ribonucleic Acid) attenuated substrate taking GFP as a reporter gene. The research finds that the P8 has a silence inhibition function, can inhibit degradation of a nonsense mediated mRNA decay substrate taking GFP as a reporter gene and degradation of an endogenous nonsense mediated mRNA decay (NMD) substrate of arabidopsis thaliana, can improve the mRNA expression quantity of AT1G0106020, RPS6, SMG7, AT5G35490, AT5G64430, AT1G36730, AT4G3900 and AT5G22570, and can reduce the expression quantity of AT1G72450 and AT2G400, and the research result lays a foundation for clarification of the function of the P8.

The invention discloses a medicine for treating osteoarthritis, and belongs to the field of medicine, the medicine comprises a reagent capable of stimulating chondrocyte development, and the medicine L-arginine can promote expression of chondrocyte anabolic factors and reduce expression of cartilage degradation markers, so that chondrocytes are protected to a certain extent; in addition, the medicine provided by the invention has an excellent cartilage repair function, can inhibit the progress of degenerative osteoarthritis, has good biocompatibility, and has no cytotoxicity to cartilage cells.

The invention relates to the technical field of biological materials, in particular to a cyclic peptide derivative and a preparation method and application thereof. The cyclopeptide derivative provided by the invention simultaneously comprises a hydrophobic cyclopeptide structure and a hydrophilic pantothenic acid structure which are combined through a covalent ester bond; due to the structure, excellent stability is achieved, and the problems that pantothenic acid is unstable and irritant are effectively solved; after entering the skin, the derivative can be hydrolyzed into pantothenic acid under the action of esterase, and can play a role without oxidative conversion of panthenol. Secondly, the cyclic peptide derivative disclosed by the invention has good biological safety; in an inflammatory cell model, the derivative can more effectively reduce expression of inflammatory factors, and antioxidant activity research shows that the derivative has a strong free radical inhibition effect and can exert excellent anti-inflammatory and antioxidant effects.

The invention discloses a fluorescent quantitative PCR (polymerase chain reaction) detection method and a detection kit for detecting differentiation potential of induced pluripotent stem cells. The detection method comprises the following steps: S1, preparing an embryoid body by utilizing iPSC (induced pluripotent stem cells); s2, extracting RNA (Ribonucleic Acid) of the iPSC and the embryoid body; s3, respectively carrying out reverse transcription on the iPSC and RNA of an embryoid body to obtain cDNA; s4, establishing a fluorescent quantitative PCR reaction system by adopting the primer composition in claim 1; s5, carrying out fluorescent quantitative PCR (Polymerase Chain Reaction) detection on the cDNA in S3; and S6, analyzing a result. The detection kit disclosed by the invention can be used for rapidly and accurately evaluating the differentiation potential of the induced pluripotent stem cells, and is suitable for large-scale cell differentiation research and clinical application.

The invention discloses combined lactobacillus salivarius CCFM1398 for regulating and controlling bone homeostasis and relieving bone resorption and a metagen of the combined lactobacillus salivarius CCFM1398, and belongs to the technical field of microorganisms. The combined lactobacillus salivarius CCFM1398 provided by the invention is separated from oral dental plaque of healthy teenagers, has the effects of relieving bone resorption and resisting inflammation, and is specifically reflected in that expression of protein level and gene level of MC3T3-E1 cell osteoprotegerin (OPG) is improved in vitro; the bone absorption amount of orthodontic bone absorption rat alveolar bone is reduced, the number of alveolar bone osteoclasts is reduced, and the level of rat serum OPG is improved; the secretion of inflammatory factors TNF-alpha, IL-6 and MPO of the orthodontic bone absorption rat is reduced; and the pathological condition of orthodontic bone absorption rat alveolar bone tissue is improved. Therefore, the combined lactobacillus salivarius has a huge application prospect in products for relieving bone resorption and assisting in inhibiting periodontal tissue inflammation.

The invention belongs to the field of biological materials, and particularly relates to a multifunctional DNA nanometer material, a preparation method and application of the multifunctional DNA nanometer material in treatment of traumatic brain injury secondary encephaledema. The multifunctional DNA nano material comprises a plurality of deoxyribozyme (DNAzyme) targeting C1q mRNA (messenger Ribonucleic Acid) and a repeating unit which is integrally connected with G-quadruplex / Hemin in series, wherein the G-quadruplex / Hemin is labeled by streptavidin SA and biotin and is used for scavenging free radicals. The DNAzyme for silencing the C1q gene of the mouse is designed on the basis of a bioinformatics method, and the C1q gene overexpressed in an inflammation model can be knocked down to an approximate normal level. The RCA technology mediated self-assembled nano material SA-B / H has excellent enzyme mimetic activity, can effectively remove free radicals such as H2O2 and O2 <.->, and has the potential of in-vivo oxidation resistance. In-vitro experiments prove that SA-B / H can effectively relieve high-level oxidative stress of cells caused by H2O2 stimulation.

The invention relates to an application of chrysin in preparation of drugs for treating mammalian NFAT2 related diseases. It is confirmed for the first time that chrysin shows significant anti-inflammatory activity in a KD mouse model and a cell injury model. This effect may be achieved by modulating NFAT2. The discovery has important significance for understanding and potentially treating the vascular endothelial injury of the Kawasaki disease, and a new insight is provided for treating the Kawasaki disease by using traditional Chinese medicines.

The invention belongs to the field of artificial intelligence, and relates to an image generation model training method comprising the following steps: obtaining a sample image and structural feature information corresponding to the sample image; inputting the structural feature information as a constraint condition into a preset diffusion model to generate a plurality of prediction images; based on an intra-class contrast learning strategy, performing integration processing on the plurality of prediction images and the sample image to obtain a loss function; adjusting parameters of the diffusion model based on the loss function, and returning to execute the step of inputting the structural feature information as the constraint condition into the preset diffusion model until the loss function is obtained until the convergence of the loss function reaches a convergence threshold value; and determining the diffusion model corresponding to the condition that the loss function reaches a convergence threshold as an image generation model. The invention further provides a device, equipment and a medium. In addition, the invention also relates to a block chain technology, and the sample image and the structural feature information can be stored in a block chain. According to the invention, the image generation accuracy can be improved.

The invention discloses a combined lactobacillus salivarius CCFM1397 for improving osteoblast activity and relieving bone resorption and periodontal tissue damage and a metagen thereof, and belongs to the technical field of microorganisms. The lactobacillus salivarius combination CCFM1397 provided by the invention is separated from oral dental plaque of healthy teenagers, has the effects of relieving bone resorption and resisting inflammation, and is specifically reflected in that: the expression of MC3T3-E1 cell osteoprotegerin (OPG) protein and gene level is improved in vitro; the bone absorption amount of orthodontic bone absorption rat alveolar bone is reduced, the number of alveolar bone osteoclasts is reduced, and the level of rat serum OPG is improved; the secretion of inflammatory factors IL-1beta, IL-6 and TNF-alpha and myeloperoxidase MPO of the orthodontic bone absorption rats is reduced; and the pathological condition of orthodontic bone absorption rat alveolar bone tissue is improved. Therefore, the combined lactobacillus salivarius has a huge application prospect in products for relieving bone resorption and assisting in inhibiting periodontal tissue inflammation.

The invention relates to the technical field of microorganisms, in particular to a lactobacillus paracasei strain and application thereof. The preservation number of the lactobacillus paracasei strain is CGMCC (China General Microbiological Culture Collection Center) No.27975. The lactobacillus paracasei disclosed by the invention can tolerate an oral environment, inhibit proliferation of streptococcus mutans and remarkably inhibit adhesion of the streptococcus mutans on a hydroxyapatite tooth model; the level of producing water-insoluble exopolysaccharides by streptococcus mutans is reduced, the formation of a dental plaque biofilm caused by streptococcus mutans is effectively reduced, the content of a mature dental plaque biofilm is reduced, and the biofilm is not easy to form; the expression quantity of cariogenic genes gtfB, gtfC, gtfD, ftf and LuxS of streptococcus mutans can be obviously reduced.

The invention discloses a targeted nano delivery system for pulmonary fibrosis treatment and a preparation method of the targeted nano delivery system. The lung targeting nano delivery system comprises a carrier and a substance A, the carrier is a nano-particle formed by TM2 or a nano-particle formed by TM2 and DOPE (Dioctyl Olefin Polyethylene); the structural formula of the TM2 is shown as a formula I; the substance A is selected from at least one of the following substances: 1) a substance for inhibiting the expression of a TTC3 gene, 2) a substance for knocking out the TTC3 gene, and 3) a substance for inhibiting the activity or content of the TTC3. The targeted nano-delivery system can reach the deep part of lung tissue through oral trachea administration to realize the effect of safely and effectively delivering drugs.

The invention discloses application of CREB3L1 in diagnosis and treatment of systemic sclerosis, and belongs to the technical field of biological medicine. The invention discovers that CREB3L1 has the function of regulating and controlling fibrosis of skin and lung tissues of a patient with systemic sclerosis for the first time, and plays an important role in the progress of fibrosis of systemic sclerosis. The CREB3L1 can be used as a biomarker for diagnosis and auxiliary diagnosis of systemic sclerosis, and the CREB3L1 inhibitor can be used as an effective component for controlling and / or reversing fibrosis of systemic sclerosis, and has a good clinical application prospect. A new target is provided for diagnosis and treatment of systemic sclerosis, and a solid research foundation is laid for development of drugs for resisting systemic sclerosis fibrosis.

The invention discloses application of lactobacillus muricatum in preparation of a medicine for preventing and / or treating depression in the technical field of microorganisms, and the lactobacillus muricatum is preserved in the China Industrial Microbiological Culture Collection Center (CICC) with the preservation number of CICC23140. Research finds that lactobacillus muricatum which is preserved in the China Industrial Microbiological Culture Collection Center and has the preservation number of CICC23140 has a good effect of improving and / or preventing and / or treating cortisol-induced depression, and inactivated lactobacillus muricatum can effectively prevent hippocampal neuron damage, reduce expression of inflammatory factors in the brain, prevent and treat cortisol-induced depression, and can be used for preventing and treating cortisol-induced depression. Not only can the anxiety symptom of depression be improved, but also dyskinesia can be relieved.

The present invention relates to a pharmaceutical composition for the prevention or treatment of inflammatory disease, comprising mesenchymal stem cells expressing PTX-3, TIMP1, and BDNF as an active ingredient. The mesenchymal stem cells expressing PTX-3, TIMP1, BDNF, and / or HLA-A2, according to the present invention, may inhibit an inflammatory response by reducing the expressions of the inflammatory cytokines IL-1b, IL-6, IL-8, and TNF-a, and increasing the expressions of the anti-inflammatory cytokines IL-10 and arginase-1 (ARG-1), and may relieve pain caused by osteoarthritis when administered in an osteoarthritis-induced rat. Thus, the mesenchymal stem cells expressing PTX-3, TIMP1, and BDNF of the present invention may be usefully employed in the prevention, alleviation, or treatment of inflammatory disease, pain, and pain cuased by inflammation.

The invention discloses an oligopeptide for treating neonatal bacterial meningitis and a preparation method of the oligopeptide, and belongs to the technical field of biological medicines. The invention successfully designs and prepares small molecular mimic peptides capable of effectively blocking escherichia coli from invading into the brain based on a lactam cyclization technology, the small molecular mimic peptides are named as EC-MP-1 and EC-MP-2, and animal level research proves that the mimic peptides disclosed by the invention can inhibit the invasion of E44 escherichia coli to HBMEC; cell and molecular level researches prove that the mimic peptide disclosed by the invention can play an effective therapeutic role. A new thought is provided for prevention and treatment of neonatal meningitis, and reliable scientific data is accumulated for subsequent development and clinical application of micromolecule polypeptide drugs.

This invention discloses the application of Uncaria rhynchophylla neutral polysaccharide URP-W in the preparation of anti-glioma drugs, belonging to the field of anti-tumor technology of medicinal plant polysaccharides. The invention obtains a crude polysaccharide extract from Uncaria rhynchophylla through hot water extraction and ethanol precipitation, followed by purification using a DEAE cellulose column and G200 dextran gel to obtain Uncaria rhynchophylla neutral polysaccharide URP-W. The monosaccharide composition of URP-W includes arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid, and mannuronic acid in a molar ratio of 17.99:6.32:21.71:27.17:2.56:7.57:16.20:0.49. Experiments have shown that URP-W can inhibit the proliferation of gliomas and induce apoptosis, exhibiting significant anti-tumor activity against glioma cells, providing a new drug raw material and treatment method for the clinical treatment of gliomas.

The present invention belongs to a new use of verrucine, and particularly relates to the use of verrucine and verrucine extract in the preparation of antiviral drugs. Verrucine has an inhibitory effect on porcine delta coronavirus, porcine epidemic diarrhea virus, pseudorabies virus, herpes simplex virus, influenza virus or African swine fever virus. The verrucine extract and verrucine of the present invention are very effective in antiviral treatment. The verrucine extract and verrucine of the present invention are very effective in broad-spectrum antiviral treatment, are safe, have few toxic and side effects, have low drug residues and are pollution-free.

The invention provides a rhizoma bletillae composition as well as a preparation method and application thereof, and relates to the technical field of traditional Chinese medicine preparations. The core effective component of rhizoma bletillae is rhizoma bletillae polysaccharide, the rhizoma bletillae polysaccharide has the effects of film forming, hemostasis, targeted wound repair promotion and infection prevention, tea polyphenol and rhizoma bletillae are compounded for use, the tea polyphenol and rhizoma bletillae synergistically resist inflammation, the expression quantity of proinflammatory factors IL-1beta, TNF-alpha and IL-6 is reduced by inhibiting NF-kappa B and MAPK signal channels, the inflammatory cascade reaction is remarkably blocked, and the anti-inflammatory effect is achieved. Red, swollen and pain caused by inflammation are effectively relieved, and wound healing is accelerated. In addition, the rhizoma bletillae and the tea polyphenol can also generate a synergistic antibacterial effect, and compared with a single rhizoma bletillae component, the probability of wound infection is further reduced; the compounding use of the rhizoma bletillae and the tea polyphenol also inhibits the excessive deposition of collagen, reduces the scar area, reduces the scar hyperplasia degree, and makes up the blank of only promoting healing and not preventing scars for burns and scalds in the prior art.

The TaSL1 protein or biological material provided by this invention has the function of increasing the number of grains per ear and the length of the ear in wheat. After reducing the expression of TaSL1 protein in wheat, the transgenic wheat obtained has a significantly increased number of grains per ear, ear length, and awn length compared with the control. The TaSL1 protein or biological material provides a new genetic resource for breeding wheat varieties with high grain count and long ears, which is of great significance for improving wheat yield.

The invention discloses a fish scale collagen skincare composition, a fish scale collagen cosmetic and a preparation method of the fish scale collagen skincare composition and the fish scale collagen cosmetic. The fish scale collagen skincare composition comprises 0.05-0.5 part of fish scale collagen, 0.05-0.5 part of madecassoside and 0.5-3 parts of a purslane extract. According to the invention, the fish scale collagen, madecassoside and purslane extract are combined to repair the skin barrier, calm and relieve nerves, relieve skin inflammatory response and improve sensitive skin in multiple aspects from the aspects of sensitive skin characteristics, possible mechanisms and the like instead of treating head pain and foot pain. The fish scale collagen, madecassoside and purslane extract are derived from fish scales, centella asiatica and purslane of perches respectively, are gifts given to human beings by the nature, are green and healthy, and can endow the perches with high economic value, and through the simple synergistic effect of the three components and the extremely small component addition amount, the basic state of the skin of sensitive skin is consolidated, and the skin care effect is improved. The symptoms of itching, redness and the like after the sensitive skin is stimulated are relieved.

The present invention relates to the field of medical technology, and provides a PMC nanozyme and a preparation method thereof; the PMC nanozyme comprises: a core layer, the core layer comprises oxygen atoms, phosphate ions and polyoxometalate nanoclusters of a first transition metal element; a coating layer, at least part of the coating layer is fixed on the outer surface of the core layer, and the coating layer is formed by the coordination between a second transition metal and the oxygen atoms on the core layer; wherein the first transition metal element comprises Mo; the second transition metal element comprises Mn, and the coating layer comprises a CO donor for controlling the release of CO gas. The PMC nanozyme of the present invention can utilize polyoxometalate nanoclusters in combination with CO gas therapy to synergistically treat IBD, and on the basis of endowing the nanoclusters with anti-inflammatory and intestinal epithelial barrier repair functions, the catalytic activity is enhanced, and CO is released in an environment of overexpression of reactive oxygen species, thereby alleviating oxidative stress, inhibiting inflammatory responses, restoring intestinal immune balance, and promoting the repair of mucosal damage.

The invention relates to the field of biology, in particular to shRNA for inhibiting chicken lnc-CA13 gene expression, a recombinant vector, recombinant bacteria and application of the shRNA. The invention provides shRNA (short hairpin Ribonucleic Acid) for inhibiting expression of the chicken lnc-CA13 gene, and constructs a corresponding shRNA lentiviral expression vector and recombinant bacteria. The nucleotide sequences of the three shRNAs provided by the invention are shown as SEQ ID NO.5-10, the three shRNAs can effectively reduce the expression quantity of the chicken lnc-CA13 gene, and the inhibition efficiencies at the gene level are respectively 27.88%, 54.58% and 39.42%. ShRNA for inhibiting chicken lnc-CA13 gene expression is constructed on a lentiviral vector, and the lentiviral vector is suitable for in-vivo and in-vitro research; meanwhile, the invention has important scientific significance and application prospects for deeply researching the functions of the chicken lnc-CA13 gene and the like.

The present invention relates to a compound containing a pyrrolidine moiety as a linker or a pharmaceutically acceptable salt thereof. The present invention also relates to a composition for preventing and treatment of the respiratory diseases or the skin conditions such as atopy and psoriasis and bronchial diseases, including the compound as an active ingredient.

The invention relates to a creation method and application of uORF for regulating and controlling MSTN protein expression. The invention provides a pig MSTN gene containing a newly created upstream open reading frame (uORF) in a non-coding region (5 'UTR). The invention also provides the sgRNA for creating the upstream open reading frame at the 5 'UTR of the pig MSTN gene and a gene editing system. The invention also provides a method for regulating and controlling the pig MSTN gene, a pig fetus fibroblast with reduced MSTN protein expression level and a production method of a lean type pig. The system and the method provided by the invention can realize low expression of the MSTN protein, so that a lean type pig model can be created for livestock breeding improvement, and the system and the method are efficient and safe.

The present invention discloses a mint McTRY gene, its expression protein and application, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the mint McTRY gene disclosed in the present invention is shown in SEQ ID NO.1; the amino acid sequence of its expression protein is shown in SEQ ID NO.2. The present invention uses mint leaves as materials, obtains the mint McTRY gene by cloning, constructs its overexpression vector 35S::McTRY on this basis, and transfers it into Arabidopsis to obtain transgenic plants. No epidermal hairs are formed on the leaves and stems of the transgenic strains, and the anthocyanin content is extremely low. The McTRY gene can down-regulate the expression of positive regulatory genes for epidermal hair formation, down-regulate the expression of anthocyanin synthesis genes, down-regulate the expression of related transcription factors for anthocyanin synthesis, and up-regulate the expression of negative regulatory genes for epidermal hair formation at the molecular level.