104 results about "Total cholesterol" patented technology

The invention discloses application of an overexpressed carp lpla gene in regulation and control of expression of cyprinid fish tissue-related genes, and relates to the field of gene engineering, in particular to application of the overexpressed carp lpla gene in regulation and control of cyprinid fish tissue-related genes. The overexpressed carp lpla gene is applied to regulation and control of contents of lipid indexes of total cholesterol TC and triglyceride TG of different tissues of cyprinid fishes. The invention further discloses application of the overexpressed carp lpla gene in regulation and control of fat marker genes lpla, ppar gamma, Fabp3, fast and acaca of livers and muscle tissues of cyprinid fishes. The invention further discloses application of the overexpressed carp lpla gene in regulating and controlling the relative expression quantity of carp liver cell fat metabolism related genes lpla, ppar gamma, Fabp3, fast, acaca, hsl and pnpla2. The overexpressed carp lpla gene can regulate and control lipid deposition in different tissues in zebra fish, and a research model is provided for lpla to regulate and control fat deposition and differentiation. According to the overexpressed carp lpla gene disclosed by the invention, lpla high-expression carp hepatocytes are obtained through a liposome transfection technology, so that fat key gene expression in the carp hepatocytes is changed.

The invention discloses hippophae rhamnoides polysaccharide with the activity of reducing blood sugar and blood fat as well as a preparation method and application thereof. Adding water into the degreased hippophae rhamnoides, adjusting the pH value to be acidic, then heating, stirring and extracting under an ultrasonic condition, and collecting filtrate; and performing post-treatment to obtain the sea-buckthorn polysaccharide. The content of neutral sugar in the sea buckthorn polysaccharide is 25.31%-30.68%, the content of uronic acid in the sea buckthorn polysaccharide is 54.66%-59.79%, and the content of protein in the sea buckthorn polysaccharide is 1.91%-2.14%. According to the method, the sea-buckthorn polysaccharide is extracted by adopting acid assistance-ultrasonic extraction, and the prepared sea-buckthorn polysaccharide has remarkable blood sugar and blood fat reducing activity. When the concentration of the sea-buckthorn polysaccharide is as low as 0.1 mg / mL, the glucose uptake of HepG2-IR cells can still be obviously improved, and the content of total cholesterol and total triglyceride of the HepG2-IR cells can be obviously reduced. The hippophae rhamnoides polysaccharide can be applied to preparation of food or medicine for reducing blood sugar and / or blood fat.

The invention provides a lipid-lowering traditional Chinese medicine composition and extract as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The lipid-lowering traditional Chinese medicine composition is prepared from the following raw materials: rhizoma cyperi and radix gentianae. The invention further provides an extract of the lipid-lowering traditional Chinese medicine composition. The extract is obtained by mixing rhizoma cyperi and radix gentianae and then co-extracting. The composition or the extract can be used for preparing medicines for preventing and / or treating hyperlipemia, and can be used for improving lipid metabolism disorder of liver cells and reducing the content of triglyceride and total cholesterol in the liver cells.

The invention belongs to the technical field of biological medicines, and particularly relates to application of a PPARalpha agonist combined with an NPC1L1 inhibitor in tumor treatment. Specifically, it is proved for the first time that systemic PKM2 deficiency can cause increase of the total cholesterol level and promote growth of homologous allograft tumors, and the phenomenon is partially attributed to increase of the expression level of intestinal NPC1L1. In addition, in breast cancer cells, PKM2 gene knockout not only weakens the activity of a PPARalpha signal channel, but also improves the expression of NPC1L1. Fenofibrate and Ezetimibe can be used for cooperatively inhibiting proliferation and metastasis of tumor cells in vitro and in vivo, so that the Fenofibrate and Ezetimibe have important clinical significance and social value.

Methods and compositions using Dichrostachys glomerata or Cissus quadrangularis separately to provide a variety of health benefits, including but not limited to, increase GLP-1 levels and reduce or decrease visceral fat, food intake, blood lipids, total cholesterol and total glucose in a mammal. In one embodiment, a composition for reducing visceral fat in a mammal is provided where the composition comprises an effective amount of Cissus quadrangularis provided as an oral dosage unit in the form of a pill, capsule, liquid, lozenge or tablet.

The invention discloses a functional solid beverage capable of reducing fat, losing weight and conditioning five highs. According to the functional solid beverage, dietary fibers such as fructo-oligosaccharide, inulin and the like are used for regulating intestinal flora, a white kidney bean extract is used for inhibiting the activity of alpha-amylase, L-carnitine is used for promoting fat transport, and medicinal and edible components such as ginseng, lotus leaves and the like are matched to realize multi-way metabolism regulation. The weight percentage of each component can be adjusted within a specified range so as to adapt to different physique requirements. Animal experiments prove that the total cholesterol (TC) of serum can be obviously reduced by 21.3-25.8% and the triglyceride (TG) can be obviously reduced by 28.9-34.2% after the traditional Chinese medicine is continuously taken for 4 weeks, and meanwhile, the insulin resistance index (HOMA-IR) can be improved to 16.5-20.3%.

The invention discloses application of lactobacillus fermentum E1 in preparation of fat-reducing and weight-losing products, and belongs to the technical field of microorganisms. The lactobacillus fermentum E1 disclosed by the invention can remarkably promote fat metabolism in the zebra fish body in vivo, can remarkably reduce the content of triglyceride and total cholesterol in the hyperlipidemia zebra fish body, and has the potential of being applied to losing weight and regulating the blood fat level; theoretical reference and guidance basis are provided for developing probiotic preparations for treating or preventing obesity and hyperlipidemia by using the lactobacillus fermentum E1.

The application provides a method, application and product for preparing a triglyceride oil with five effects of improving blood vessel health by enzymolysis, and belongs to the technical field of enzyme engineering. The preparation method comprises the following steps: mixing low erucic acid rapeseed oil, glycerol, water, fatty acid and lipase, performing enzymolysis reaction, performing molecular distillation, and performing decolorization and deodorization to obtain the triglyceride oil; the lipase is a mixture of Candida antarctica black Aspergillus niger lipase, Candida cylindracea lipase and bovine pancreas lipase; and the weight ratio of the low erucic acid rapeseed oil, glycerol, water, fatty acid and lipase is 500:80-100:1-2:30-60:10-20. The triglyceride oil prepared by the method can be used to prepare a triglyceride oil composition, and has the five effects of lowering blood uric acid, lowering blood total cholesterol, lowering blood triglyceride, lowering blood sugar and improving blood flow speed, thereby improving blood vessel health.

The invention provides application of golden amide alcohol ester or pharmaceutically acceptable salt thereof in preparation of medicines for preventing / treating fatty liver diseases and complications thereof. Compared with the prior art, the application has the following advantages: the application verifies that the auramide ester can obviously reduce the content of total cholesterol (TC) and triglyceride (TC) of a metabolism-related fatty liver disease model mouse, inhibit the activity of alanine transaminase (ALT), aspartate transaminase (AST) and low-density lipoprotein (LDL), inhibit the metabolism-related fatty liver disease model mouse, inhibit the metabolism-related fatty liver disease model mouse metabolism-related fatty liver disease mouse metabolism-related fatty liver disease mouse metabolism-related fatty liver disease mouse metabolism-related fatty liver disease mouse metabolism-related fatty liver disease mouse metabolism-related fatty liver disease mouse metabolism-related fatty liver disease mouse metabolism-related fatty liver disease mouse metabolism-related fatty Compared with the prior art, the invention has the advantages that the golden amide alcohol ester has obvious multiple effects of lowering lipid, resisting inflammation, resisting fibrosis and protecting liver, is basically non-toxic and good in safety, can be used for preparing the medicine for preventing / treating metabolism-related fatty liver diseases and related complications thereof, and has a wide application prospect.

The present application provides a polypeptide composition with anti-obesity effect, which is composed of capsaicin and polypeptides, and the amino acid sequence of the polypeptides comprises any one or more of SEQ ID NO. 1 to SEQ ID NO. 8. The polypeptides of sequences 1-8 are mixed uniformly at a mass ratio of 1-20:1-4:1-2:1-4:1:1:1:1, and the capsaicin is mixed with the mixed polypeptides at a ratio of 2-12:100 (w / w). The polypeptide composition proposed in the present application, in combination with capsaicin, not only promotes the weight loss effect of capsaicin, but also significantly reduces the fat index of the test mice after use, and can significantly reduce the total cholesterol concentration in the liver and the liver triglyceride concentration under a high-fat diet.

The invention discloses a compound polysaccharide preparation for treating diabetes and application thereof, and belongs to the technical field of biomedicine. The compound polysaccharide preparation is composed of compound polysaccharide A and compound polysaccharide B according to the mass ratio of 1: 1. The compound polysaccharide A is prepared from abelmoschus esculentus and chlorella through alkaline ultrasonic extraction and 10-100kDa ultrafiltration classification; the compound polysaccharide B is prepared by performing enzymolysis and hot water extraction on grifola frondosa and sarcodon aspratus. Experiments prove that the preparation can significantly improve insulin secretion and glucose uptake ability of islet cells, effectively reduce fasting blood-glucose of type 2 diabetes model mice and synchronously improve triglyceride and total cholesterol levels through strong synergistic interaction among the components. The preparation disclosed by the invention is natural in source, safe and efficient, so that a new choice is provided for medicine development of type 2 diabetes mellitus.

The invention discloses application of lactobacillus gasseri LS03 in preparation of fat-reducing and weight-losing products, and belongs to the technical field of microorganisms. The lactobacillus gasseri LS03 disclosed by the invention can remarkably promote fat metabolism in a zebra fish body in vivo, can remarkably reduce the content of triglyceride and total cholesterol in a hyperlipidemia zebra fish body, has the potential of being applied to losing weight and regulating the blood fat level, and can be used for preventing and treating hyperlipidemia. The lactobacillus gasseri LS03 provides theoretical reference and guidance basis for developing probiotic preparations for treating or preventing obesity and hyperlipidemia by utilizing the lactobacillus gasseri LS03.

Methods and compositions using Dichrostachys glomerata or Cissus quadrangularis separately to provide a variety of health benefits, including but not limited to, increase GLP-1 levels and reduce or decrease visceral fat, food intake, blood lipids, total cholesterol and total glucose in a mammal. In one embodiment, a composition for reducing fasting blood glucose in a mammal is provided where the composition comprises an effective amount of Dichrostachys glomerata provided as an oral dosage unit in the form of a pill, capsule, liquid, lozenge or tablet.

The main purpose of the present invention is to provide monascus and a composition for preventing or treating non-alcoholic fatty liver disease, the composition comprising the monascus and / or a monascus fermentation product thereof, or a functional component comprising the monascus fermentation product. The invention also aims to provide application of the monascus in preparation of the composition for preventing or treating the non-alcoholic fatty liver disease. The composition not only has the efficacy of reducing accumulation of total cholesterol (TC) and triglyceride (TG) in the non-alcoholic fatty liver disease caused by obesity, but also has the efficacy of reducing serum liver function indexes of asparaginic acid transaminase (AST) and alanine transaminase (ALT), so that the composition has the efficacy of preventing or treating the non-alcoholic fatty liver disease and related metabolic diseases thereof. , for example hyperlipidemia.

The application discloses a Hypsizygus marmoreus extract and a preparation method and application thereof. The extract is prepared from the Hypsizygus marmoreus through a method combining water extraction, alcohol precipitation, macroporous adsorption resin and silica gel column chromatography, UPLC-ESI-MS / MS analysis identifies 830 compounds, and 26 main compound components with a relative content of more than 0.2%; network pharmacology analysis obtains 40 active compounds and 23 T2MD related core target points. The extract can significantly reduce fasting blood glucose and postprandial blood glucose, can significantly reduce serum total cholesterol, triglyceride and low-density lipoprotein cholesterol levels, can improve fatty liver level, can significantly reduce serum inflammatory factors IL-6, TNF-alpha and CRP levels, can significantly increase GLP-1 and serum insulin levels, and can improve intestinal flora alpha diversity, improve beta diversity structure and restore the abundance of beneficial bacteria. The Hypsizygus marmoreus extract has multi-target and multi-metabolic regulation functions, can be used for developing a blood glucose and blood lipid reducing medicine or health food, and has high safety and definite effect.

The application relates to the field of biological medicine, and particularly relates to application of kaempferol-3-O-acacia di-saccharide-7-O-rhamnose glycoside in preparation of a medicine for preventing and / or treating diseases related to glycolipid metabolism disorder. The compound or a pharmaceutically acceptable salt, ester, solvate or prodrug thereof can be used for treating diseases such as type 2 diabetes, insulin resistance, hyperglycemia, hyperlipidemia and non-alcoholic fatty liver disease. The compound can significantly reduce intracellular glucose, triglyceride and total cholesterol levels, and can play a role by activating an AKT signal pathway, so as to improve glycolipid metabolism disorder and has potential clinical application value.

The application belongs to the technical field of biological medicine, and particularly relates to application of a GCN2 kinase inhibitor in preparation of a drug for treating heart failure with preserved ejection fraction. 18 H 12 ClF2N5O3S, the inhibitor can inhibit the activity of GCN2 kinase, and exhibits significant therapeutic effect in a disease model: can obviously improve ventricular diastolic function (E / E' ratio is reduced), reduce the level of heart failure markers, reduce cardiomyocyte hypertrophy and interstitial fibrosis, inhibit the expression of oxidative stress level and inflammatory factors, reduce the accumulation of triglyceride and total cholesterol in myocardium and serum, and improve obesity-related insulin resistance and metabolic disorder. The application provides a novel targeted treatment strategy and candidate drug for heart failure with preserved ejection fraction.

The invention discloses an application of a jerusalem artichoke tuber alcohol extract in preparation of a medicine for treating type 2 diabetes mellitus, and belongs to the technical field of medicines, the chemical components of the jerusalem artichoke tuber alcohol extract are mainly flavonoid substances, and the jerusalem artichoke tuber alcohol extract can remarkably recover the weight of a mouse with the type 2 diabetes mellitus and improve glucose metabolism indexes such as fasting blood-glucose and oral glucose tolerance; meanwhile, insulin sensitivity is stimulated to secrete insulin, and the functions of pancreatic beta cells are recovered; in addition, the concentration of biochemical indexes such as total cholesterol and triglyceride in the liver is reduced, lipid metabolism of the liver is improved, and a new direction is provided for research and development of natural plant source hypoglycemic products.

The invention belongs to the field of gene editing, and particularly relates to a method for regulating milk fat content. The expression of genes in fatty acid and cholesterol synthesis pathways of the livers of the animals in the lactation period is specifically regulated and controlled through a gene delivery technology, the expression of liver apolipoprotein B (ApoB) and the triglyceride and total cholesterol level in blood can be remarkably reduced, then the lipid content of milk is effectively reduced, meanwhile, the food intake of the animals is reduced, and the production cost is reduced. And the milk protein content is not influenced. According to the method, milk fat components are accurately controlled through maternal liver gene editing for the first time, a new strategy is provided for breeding healthy dairy animals, and the method has wide application prospects.

Methods and compositions using Dichrostachys glomerata or Cissus quadrangularis separately to provide a variety of health benefits, including but not limited to, increase GLP-1 levels and reduce or decrease DPP-4 activity or levels, visceral fat, food intake, blood lipids, total cholesterol and total glucose in a mammal. In one embodiment, a composition for reducing or decreasing DPP-4 activity or levels in a mammal is provided where the composition comprises an effective amount of Dichrostachys glomerata provided as an oral dosage unit in the form of a pill, capsule, liquid, lozenge or tablet.

The invention relates to the technical field of biological medicines, in particular to saponin compounds separated from quinoa husks, and the compounds have remarkable lipid-lowering and blood-glucose-lowering effects, can effectively inhibit the expression of adipogenesis related proteins (such as FAS, ACC and SCD-1), and reduce the levels of triglyceride (TG), total cholesterol (TC) and glucose in cells.

The invention provides a composition and application thereof in preparation of a product for maintaining the healthy level of blood lipid, the composition comprises 0.1-10 parts by weight of pentadecanoic acid, and also comprises 1 part by weight of ginseng and / or 0.1-5 parts by weight of hawthorn, the composition is clear in target spot, the components are mutually matched and synergistically interact, and the composition has a good effect on maintaining the healthy level of blood lipid. The health level of blood fat can be maintained, and two key indexes of total cholesterol and triglyceride can be effectively intervened at the same time; the composition is clear in mechanism and remarkable in synergism, has outstanding advantages in the aspects of safety, cost controllability and productization feasibility, and provides important material basis and technical support for developing a blood fat health management scheme which is efficient, balanced and easy to popularize.

The invention relates to the technical field of coreopsis tinctoria refined components and preparation and application, and provides a coreopsis tinctoria refined component and a preparation method and application thereof.The coreopsis tinctoria refined component comprises, by mass, 11 specific components of phenolic acids, flavonol, flavanol and flavonoid, and the 11 specific components comprise, by mass, 2 parts of gallic acid, 1 part of potassium sorbate, 1 part of potassium sorbate, 1 part of potassium sorbate and 1 part of potassium sorbate. The health-care food is prepared from the following raw materials in parts by weight: 3 parts of chlorogenic acid, 14 parts of quercetin, 18 parts of catechin, 5 parts of citric acid, 2-3 parts of procyanidine B, 3 parts of (+)-epicatechin, 1-31 parts of 31 parts of procyanidine C, 14 parts of rutin, 4 parts of camphanol-3-O-rutinoside and 2 parts of isorhamnetin-3-O-rutinoside. The snow chrysanthemum is Changbai Mountain snow chrysanthemum. Animal experiments show that the refined component can significantly reduce the content of total cholesterol and triglyceride in serum of a high-fat model rat, the specific component with blood fat regulating activity is prepared from Changbai Mountain snow chrysanthemum, and a new choice is provided for non-drug intervention of dyslipidemia.

The invention discloses a capsule medicine for treating hyperlipemia as well as a preparation method and application thereof, and belongs to the technical field of hot-dip galvanized steel plates, the capsule medicine is prepared from the following raw materials in percentage by weight: 58-69% of egg extract, 8.3-10.3% of tea polyphenol, 13.2-18.6% of sophora flavescens extract and 7.1-13.1% of turmeric extract. Through the mode, the egg extract improves antioxidant indexes of liver VLDL, superoxide dismutase, glutathione peroxidase, total antioxidant capacity and the like and reduces accumulation of plasma triglyceride, total cholesterol and the like, the tea polyphenol and the sophora flavescens extract are beneficial for improving the activity of the egg extract, the effect of the egg extract is further improved, and meanwhile, the effect of the egg extract is further improved. In addition, the tea polyphenol can inhibit the generation of fat and effectively reduce energy accumulation, and in addition, the turmeric extract is beneficial to accelerating the rapid discharge of triglyceride and total cholesterol after removal, so that the treatment of hyperlipidemia is realized.

The application discloses a compound probiotic for lowering blood lipid and regulating constipation and application of the compound probiotic in resistant starch fermented milk, and belongs to the technical field of functional food. The compound probiotic is composed of Bifidobacterium animalis IU100 and Lacticaseibacillus paracasei ZYhyy-004, and has a synergistic effect. The compound probiotic is used for preparing resistant starch fermented milk, can significantly reduce the levels of serum total cholesterol, triglyceride and low-density lipoprotein cholesterol, increase the content of high-density lipoprotein cholesterol, enhance the intestinal propulsion rate and improve functional constipation. The fermented milk can regulate intestinal flora balance, promote the proliferation of beneficial bacteria and inhibit the growth of pathogenic bacteria, and realizes the dual effects of lowering blood lipid and improving intestinal function. The application provides a new way for developing multifunctional probiotic food.

The present invention relates to a composition for regulating hepatic lipid metabolism, comprising Lactiplantibacillus plantarum OPT-A1 strain deposited under accession number KCTC 15555BP, a culture liquid thereof, a cultured product thereof, or a culture lysate thereof. The composition suppresses lipid accumulation and expression of genes related to lipogenesis in hepatocytes, and enhances expression of genes related to lipid oxidation. In addition, the composition inhibits accumulation of triglycerides in hepatocytes and reduces blood triglyceride levels, total cholesterol levels, and liver injury indices. Furthermore, the composition promotes rapid consumption of lipids in hepatocytes and somatic cells through enhancement of muscle mass, and thus can be advantageously used as a raw material for foods, pharmaceuticals, and the like related to alleviation and treatment of various metabolic diseases.

The application discloses application of calyculostero in preparation of blood sugar reducing drugs, and belongs to the technical field of pharmacological action of calyculostero. Experimental results show that the calyculostero can inhibit the activity of pancreatic alpha-amylase (IC50 is 4.34+ / -1.10 ug / mL) in a dose-dependent manner, reduce postprandial blood sugar, and improve sugar tolerance, reduce fasting blood sugar, improve total cholesterol and triglyceride levels, and reduce histopathological changes of liver, pancreas and kidney in long-term intervention, and has no obvious hepatotoxicity. The calyculostero can be used alone or together with a pharmaceutically acceptable excipient to prepare a pharmaceutical composition, and is used for reducing blood sugar or treating diabetes, and the dosage form includes oral preparations, injections and the like. The application provides a new natural medicine selection for drug treatment of diabetes.

The invention discloses a blood fat reducing compound based on sea cucumber peptide as well as a preparation method and application thereof, and belongs to the technical field of health food. The compound comprises the following raw materials: sea cucumber peptide, a zingiber mioga bud aqueous extract, a dandelion aqueous extract, an industrial hemp leaf aqueous extract and vitamin C. The components are compounded according to a specific ratio, and the components generate a remarkable synergistic effect and jointly act on a plurality of links of lipid metabolism. The compound can remarkably reduce the levels of serum total cholesterol (TC), triglyceride (TG) and low-density lipoprotein (LDL) of hyperlipidemia model mice, meanwhile, the level of high-density lipoprotein (HDL) is increased, the blood fat reducing effect of the compound is superior to that of a single component and a positive control drug, and the compound is high in safety. The invention also provides a preparation method of the compound and application of the compound in preparation of drugs or health-care foods for prevention and / or adjuvant therapy of hyperlipidemia.

The invention discloses lactobacillus plantarum ZYT028 and application thereof, the strain is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 20251257, and the lactobacillus plantarum ZYT028 has good acid cholate tolerance and intestinal colonization ability, can still keep high survival rate under the condition of pH 2.0, and can grow in an environment containing 1.0 g / L cholate. The ZYT028 strain can promote the generation of short-chain fatty acid and improve the imbalance of intestinal flora caused by high fat diet; in animal experiments, the content of serum total cholesterol and triglyceride can be remarkably reduced, excessive weight increase is inhibited, and good lipid-lowering and weight-regulating effects are shown. The strain disclosed by the invention can be widely applied to development of functional foods, health-care products, fermented dairy products and probiotic preparations, and is used for assisting in controlling body weight and improving blood lipid metabolism.