375 results about "Glycopeptide" patented technology

The invention relates to the field of cubilose peptides, in particular to preparation and application of cubilose glycopeptides capable of preventing and assisting in treating gynecological inflammation and gastrointestinal inflammation. The invention provides cubilose glycopeptide which comprises cubilose peptide, sialylated O-glycan and sialylated N-glycan, and the cubilose peptide is connected with the sialylated O-glycan and / or the sialylated N-glycan. The cubilose glycopeptide provided by the invention has unique sialylated O-glycans and N-glycans, has unique biological activity and functions and synergistic effect compared with the cubilose glycopeptide without N-glycans or O-glycans and non-sialylated cubilose peptides, and forms and enhances the inhibition effect on pathogens and the antiviral effect; therefore, the compound has unique advantages in the aspect of preventing and treating related problems. The sialic acid binding type cubilose glycopeptide has stronger antibacterial activity, especially has an inhibition effect on pathogens such as candida albicans and helicobacter pylori, and also has an antiviral effect.

The invention discloses preparation and application of antioxidative, anti-inflammatory and hypoglycemic lactoferrin peptide, and belongs to the field of food science and nutriology. According to the invention, lactoferrin is used as a raw material, hydrolysis is carried out by using a composite enzymatic hydrolysis method, and the molecular weight is obtained by ultrafiltration; the preparation method comprises the following steps: preparing a component of antioxidant, anti-inflammatory and hypoglycemic peptide from the active peptide YLSW (Tyr-Leu-Ser-Trp), carrying out freeze drying on the component to obtain peptide powder rich in antioxidant, anti-inflammatory and hypoglycemic peptides, identifying peptide fragments contained in the peptide powder by utilizing an LC-MS / MS technology, and screening out the active peptide YLSW (Tyr-Leu-Ser-Trp) with antioxidant, anti-inflammatory and hypoglycemic triple functions. The active peptide is added into the product to obtain the antioxidant, anti-inflammatory and hypoglycemic functional product.

The invention relates to the technical field of biology, in particular to glycopeptide antibiotic and application thereof. According to the invention, a biosynthetic gene cluster for synthesizing a brand new glycopeptide antibiotic skeleton is excavated, and brand new glycopeptide antibiotics Varsomycin A, B and C with novel chemical structures are obtained through heterologous expression; the traditional Chinese medicine composition has a remarkable antibacterial effect on clinically drug-resistant strains. Important fermentation strains and precursor compound molecules are provided for research and development of new drugs of glycopeptide antibiotics, and important application prospects and economic values are achieved.

The invention discloses a covalent lysosome targeting chimera based on a nucleic acid aptamer and application of the covalent lysosome targeting chimera in protein degradation and immunotherapy, and belongs to the technical field of biology. The invention designs a covalent lysosome targeting chimera with click chemical reaction activity, and the simple and efficient covalent lysosome targeting chimera is used for realizing efficient protein degradation at the levels of cells and living bodies. When the tool is used for targeted degradation of immunotherapy biomarkers, immunogenic apoptosis of tumor cells can be caused, so that killing of an immune system to tumors is more efficiently activated. Meanwhile, the covalent lysosome targeting chimera overcomes the problem of immune-related side effects confronted by existing antibodies and polysaccharide peptide drugs, and can effectively avoid the potential occurrence risk of the immune-related side effects while efficiently degrading the target protein.

To provide antioxidant peptides derived from Xuanwei ham, and to provide methods for preparation and activity measurement thereof.SOLUTION: The present invention provides an antioxidant peptide with a specific amino acid sequence derived from Xuanwei ham. The present invention uses a rigid linking peptide and a flexible linking peptide to link a hexapeptide derived from Xuanwei ham, growing the peptide chain to make it suitable for genetic recombinant expression, while increasing the proportion of a target polypeptide in a recombinant protein and significantly increasing the expression level. The rigid linking peptide has an α-helical structure, which makes it easier for the antioxidant peptide to form a stable secondary structure after serial linking, and can effectively separate the active functional domain.The flexible linking peptide is mainly composed of glycine Gly, and since it has a low molecular weight, the flexibility of a polypeptide backbone structure is ensured.SELECTED DRAWING: Figure 1

The invention discloses a hypoglycemic peptide and application thereof, and belongs to the technical field of active peptides. The hypoglycemic peptide comprises peptides of which the sequences are respectively DSVF (Asp-Ser-Val-Phe) and / or TLAE (Thr-Leu-Ala-Glu). The invention further discloses a preparation method of the hypoglycemic peptide. Animal experiments prove that the hypoglycemic peptide can assist in reducing blood sugar and improve cognitive impairment of diabetes mellitus, so that the hypoglycemic peptide can be used for preparing drugs for reducing blood sugar and improving cognitive impairment of diabetes mellitus.

The invention relates to the technical field of bioanalytical chemistry, in particular to a method for simultaneously enriching and separating glycopeptides and phosphopeptides by using a bionic hydrophilic adsorbent, which specifically comprises the following steps: step 1, preparing a polyether-ether-ketone (PEEK) framework by using a fused deposition modeling method (FDM); 2, soaking the obtained PEEK framework in an aqueous solution containing dopamine hydrochloride and tris (hydroxymethyl) aminomethane, and carrying out a reaction; step 3, then, adding a mixed solution containing hyaluronic acid methacrylate, vinyl phosphoric acid, N, N-dimethyl bisacrylamide and phenyl-2, 4, 6-trimethylbenzoyl lithium phosphate into the PEEK framework; in the invention, in the aspect of material preparation, a fused deposition modeling method (FDM) is adopted to prepare a polyether-ether-ketone (PEEK) frame, and the material has good mechanical properties and chemical stability and can bear a series of subsequent complex treatment processes without being damaged.

The invention belongs to the technical field of biology, and particularly relates to a compound for treating myocardial infarction. The chondroitin sulfate oligosaccharide is extracted from natural chondroitin sulfate, and after the chondroitin sulfate oligosaccharide is treated into an oligosaccharide chain through a chemical enzyme method, experiments prove that the chondroitin sulfate oligosaccharide is beneficial to the survival of myocardial cells. Compared with other products with similar functions, the glycopeptide polymer hydrogel formed after introduction of the polymer skeleton overcomes the problem of batch-to-batch difference of natural products and is clear in structure, and the hydrogel has excellent biological activity and does not need to entrap other active ingredients such as drugs. The effects of supporting the ventricular wall of the infarction region and promoting the survival of myocardial cells are obvious, the myocardial infarction area can be effectively limited, the thickness of the ventricular wall of the infarction region is increased, the damage of myocardial infarction to the heart is reduced, and the application prospect is very good.

The invention discloses cubilose sialic acid glycan peptide capable of improving lung inflammation and application of the cubilose sialic acid glycan peptide. The ratio of fucose modified sugar chains in the cubilose sialic acid glycan peptide is 30-60%, the ratio of sialic acid modified sugar chains in the cubilose sialic acid glycan peptide is 20-40%, the core structure of the fucose type is (HexNAc) 3 (Man) 3 (Fuc) 1, the core structure of the sialic acid type is (HexNAc) 3 (Man) 3 (Gal) 1 (Fuc) 1 (NeuAc) 1, the neutral sugar dissolution rate of the cubilose sialic acid glycan peptide is high, and the glycopeptide shown in SEQ ID NO 2 has a good inflammation improving effect.

The invention discloses a 3D printing PCL / nHA combined bionic glycopeptide hydrogel scaffold for infected bone defect substitution and a preparation method thereof, and belongs to the field of biomedical materials. The scaffold comprises a 3D printed PCL / nHA porous scaffold, the 3D printed PCL / nHA porous scaffold is provided with three-dimensional interconnected micro-channels, the mass percentage of nHA is 10-30wt%, the scaffold is further filled with a bionic glycopeptide hydrogel layer formed by mixing methylacryloylated gelatin, aldehyde bletilla striata polysaccharide, antibacterial peptide and a photoinitiator according to the ratio of 10: 4: 2: 1 and carrying out ultraviolet irradiation polymerization, phenyl lithium phosphate is adopted as the photoinitiator, and the bionic glycopeptide hydrogel layer is a bionic glycopeptide hydrogel layer. And 3D printing process parameters are accurately controlled. The preparation method comprises the steps of PCL / nHA porous scaffold preparation, pretreatment, finished product treatment, bionic glycopeptide hydrogel solution preparation, filling polymerization and the like. The scaffold disclosed by the invention integrates excellent mechanical properties, biocompatibility, antibacterial and anti-inflammatory capabilities and strong bone induction biological activity, provides an ideal material for repairing infected bone defects, and effectively overcomes many defects of a traditional skull repairing material.

The present invention provides a melittin glycopeptide and a preparation method and use thereof, belonging to the field of polypeptide drugs. The melittin glycopeptide described in the present invention has Ac‑GIGAVLKVLTTGLPALISWIKRKRQQ‑NH2 as a peptide chain, and the arginine at the 22nd and / or 24th positions is glycosylated. The present invention successfully prepares a class of melittin glycopeptides, and proves that the hemolytic effect of this class of melittin glycopeptides is greatly reduced compared to melittin, effectively solving the problem of strong hemolysis of melittin in the prior art, which is beneficial to clinical application. At the same time, the antibacterial activity and tumor cell inhibition activity of some melittin glycopeptides prepared by the present invention are significantly better than those of melittin, and have good application prospects in the research and development of preparation of antibacterial drugs and anti-tumor drugs.

The invention discloses a stropharia rugoso-annulata glycopeptide extraction method, an obtained product and application. The stropharia rugoso-annulata glycopeptide extraction method comprises the following steps: uniformly drying stropharia rugoso-annulata, crushing, sieving, extracting by adopting a microwave-assisted eutectic solvent, filtering out liquid, carrying out alcohol precipitation to obtain glycoprotein, removing free protein from the glycoprotein, decolorizing, and carrying out enzymolysis to convert the glycoprotein into a glycopeptide component. The extraction method of the stropharia rugosoannulata glycopeptide is provided for the first time, and the method is simple in process, low in cost, easy to control, higher in glycopeptide yield and suitable for industrial production. The obtained glycopeptide has excellent antioxidant activity and liver protection activity, and has certain medicinal value in the field of medicines.

The preparation method comprises the following steps: by taking natural chitosan, epsilon-polylysine and polyphenol as raw materials, firstly, respectively preparing polyphenol modified chitosan and carboxyphenylboronic acid modified polylysine through a carbodiimide coupling method, then, mixing single-component solutions of the polyphenol modified chitosan and the carboxyphenylboronic acid modified polylysine, and then, preparing the antibacterial and antioxidant glycopeptide hydrogel based on the polyphenol. And the antibacterial and antioxidant glycopeptide hydrogel is obtained by virtue of the synergistic effect of the phenolic hydroxyl group-boric acid group dynamic bond and the amino group-carboxyl group. The hydrogel disclosed by the invention has broad-spectrum antibacterial property and glucose response oxidation resistance, has self-healing capability and strong wound adhesion, is excellent in biocompatibility and can be naturally degraded; and the process is simple, convenient and controllable, large-scale production is easy to realize, the hydrogel can be used as a wound dressing for treating chronic difficult-to-heal wounds such as diabetes mellitus in a targeting manner, and integrated nursing of antibiosis, antioxidation, wound adhesion and self-repairing is realized.

Provided herein are personal care compositions for reducing the signs of aging in sensitive skin without the irritating effects associated with retinol. Such personal care compositions comprise niacinamide, Leontopodium alpinum extract, Oryza sativa lees extract, panthenol, and soy-derived glycopeptides. Use of such compositions also modulates some biochemical indicators that are associated with premature aging of skin in response to pollution.

The present invention belongs to the technical field of oral preparations, and specifically relates to a collagen composition for brightening the skin and a preparation method thereof. The composition of the collagen composition includes the following raw materials by weight: 40 to 60 parts of fish collagen peptides, 2 to 5 parts of arbutin extract, 0.1 to 0.5 parts of black pepper extract, 3 to 6 parts of glutathione, 1 to 3 parts of ellagic acid, 1 to 2 parts of shea butter, 3 to 5 parts of antioxidants, 1 to 2 parts of squalane, 1 to 2 parts of ceramide, 0.5 to 1 part of sodium bicarbonate, 5 to 10 parts of β-cyclodextrin, 1 to 3 parts of lecithin, and 0.5 to 1 part of silicon dioxide. The present invention uses polyethylene glycol grafted amino nanocellulose magnetic carriers to fix enzymes, improve enzyme thermal stability and resistance to denaturation, optimize cross-linking to retain enzyme activity, simplify recovery processes, reduce costs, improve collagen peptide production and quality, and protect active ingredients through nano- and embedding technologies to enhance absorption efficiency and product stability.

The invention relates to a method for detecting relative quantification of glycoforms in glycopeptides based on mass spectrometry and application thereof, and the method comprises the following steps: pretreating a to-be-detected product to obtain glycopeptides, performing mass spectrometry detection, and then performing data analysis; the capillary temperature of the mass spectrometric detection is 250-290 DEG C. The invention creatively finds that in the detection of glycoforms in glycopeptides, the glycoform detection result also changes along with the change of capillary temperature, and within the range limited by the invention, the proportion of glycoforms is consistent with the proportion of molecular weight glycoforms, and the detection accuracy is higher. The optimization time of the subsequent method can be shortened, and meanwhile, a clear guide is provided for the optimization direction, so that the working efficiency is greatly improved.

The invention discloses an anti-freezing glycopeptide as well as a preparation method and application thereof, and relates to the technical field of freezing resistance. The amino acid sequence of the antifreeze glycopeptide is AFP1: DWWDPGFFGFPPH, and the amino acid sequence of the antifreeze glycopeptide is as shown in SEQ ID No. 1; aFP2: YGGPKPSPVDIDDW, and the AFP2 is as shown in SEQ ID No. 2. According to the preparation method disclosed by the invention, the crab shell enzymolysis product is creatively used as a basis, a non-enzymatic glycosylation method is adopted, and the crab shell polypeptide is combined with the glucan, so that the stability and the flavor of the polypeptide are obviously improved, and the anti-freezing protection performance is improved.

The invention belongs to the field of cell culture, and relates to an MRC-5 cell serum-free medium with definite components and application thereof. Nutrient substances of the serum-free culture medium comprise amino acids, vitamins, inorganic salts, trace elements, proteins, other substances and supplements. According to the MRC-5 cell serum-free culture medium, the concentration of the amino acid, the inorganic salt, the vitamin and the serum substitute is adjusted, and meanwhile, the Chinese yam glycopeptide is added, so that adherent growth of MRC-5 cells can be effectively promoted, and the technical problem that a new variety of MRC-5 cell serum-free culture medium is lacked in the prior art is solved; the requirements of nutrient substances, carbon sources and genetic material nutrient substances required by MRC-5 cell in-vitro culture can be met.

The invention discloses a method for synchronously enriching and identifying N-linked glycopeptides and O-linked glycopeptides, and relates to the technical field of material analysis chemistry and organic chemistry, the method is characterized in that glycopeptides are enriched by adopting a column solid-phase extraction mode or a dispersive solid-phase extraction mode, and a glycopeptide sample is subjected to mass spectrometry. The glycopeptide enrichment material is a material obtained by carrying out copolymerization reaction on different types of hydrophilic monomer molecules with acrylic acid groups on the surface of porous silica gel by utilizing a reversible-fracture-chain transfer free radical polymerization reaction mechanism initiated by a matrix surface, and can be used for glycopeptide enrichment. According to the method, by controlling conditions such as pH and organic phase concentration in the enrichment process, step-by-step enrichment of N-linked glycopeptide and O-linked glycopeptide can be realized, high-selectivity enrichment of two glycopeptides in a complex mixture (high non-glycopeptide doping ratio) can be realized, and the capacity of identifying glycosylation in glycoprotein is remarkably improved.

The application relates to a synthesis method of an anti-freezing glycopeptide polypeptide and belongs to the technical field of polypeptide drug synthesis. The method comprises the following steps: 2-CL-Resin is used as a carrier resin, under the condition of adding an activating agent, the carrier resin and alanine are coupled to obtain Fmoc-Ala-2-CL-Resin; according to the amino acid sequence of the anti-freezing glycopeptide, other amino acids are sequentially coupled through a solid-phase synthesis method; after a protecting group is removed and the carrier resin is cleaved, the anti-freezing glycopeptide crude peptide is obtained; and after purification, salt conversion and freeze-drying, the anti-freezing glycopeptide polypeptide is obtained. The method has the advantages of short synthesis period, low cost, easy post-treatment, few by-products, high product yield, facilitation of large-scale production of the anti-freezing glycopeptide, and considerable economic applicative value and wide application prospect.

The application discloses a glucagon peptide-1 and glucagon receptor dual-agonistic polypeptide and application thereof, and the polypeptide structure is shown in the following general formula (I). The dual-agonistic polypeptide has dual-agonistic activity on human GLP-1 and glucagon receptors. The GLP-1 / glucagon receptor dual-agonistic polypeptide can effectively reduce blood sugar and weight, and has obvious lipid-regulating effect. The GLP-1 / glucagon receptor dual-agonistic polypeptide is stable in vivo, and is suitable to be used as an active component of drugs for diabetes, obesity, hyperlipidemia and the like.

The invention discloses a high-throughput complete glycopeptide full-automatic online enrichment and separation analysis method which comprises the following steps: firstly, building a full-automatic online enrichment and separation analysis system capable of realizing synchronization of enrichment and separation analysis through switching of a ten-way valve; the full-automatic on-line enrichment and separation analysis system is adopted to enrich complete glycopeptides in a first-needle sample, then the full-automatic on-line enrichment and separation analysis system is adopted to enrich complete glycopeptides in a next-needle sample and separate and analyze complete glycopeptides in a previous-needle sample at the same time, and the above steps are repeated. The separation analysis of the complete glycopeptide in the last needle of sample is completed; the method has the advantages that the complete glycopeptide can be quickly, fully automatically and online enriched, separated and analyzed, the operation in the enrichment process is completely controllable, the problems of high sample loss rate, poor enrichment repeatability and low liquid chromatography separation degree can be greatly reduced, the time required by the complete glycopeptide enrichment and separation analysis steps is superposed, and the analysis accuracy is greatly improved. And the time required by the whole process can be obviously reduced.

The invention belongs to the technical field of biological medicines, and particularly relates to a cyclic ester peptide compound, a producing strain thereof, a preparation method and application of the cyclic ester peptide compound in preparation of antibacterial medicines. The invention provides a cyclic ester peptide compound as shown in a formula I, a stereoisomer, a tautomer and an isotope derivative of the cyclic ester peptide compound, or a pharmaceutically acceptable salt of the cyclic ester peptide compound, a producing strain Streptomyces alboflavus sp.NX-12 of the cyclic ester peptide compound, and fermentation, separation and purification methods of the cyclic ester peptide compound and the stereoisomer, the tautomer and the isotope derivative of the cyclic ester peptide compound. The cyclic ester peptide compound has strong inhibitory activity on a variety of gram-positive bacteria, can be used for preparing antibacterial drugs, shows application potential in treatment of infection and other related diseases, and compared with the existing similar gram-positive bacterium-resistant glycopeptide antibiotic vancomycin, the cyclic ester peptide compound has the advantages that the cyclic ester peptide compound can be used for preparing the antibacterial drugs; the cyclic ester peptide compound disclosed by the invention has an equivalent antibacterial effect. The preparation method of the cyclic ester peptide compound is simple in process, short in time consumption, capable of greatly reducing the medicine cost and suitable for large-scale production. # imgabs0 #

The present disclosure provides methods, compositions, and kits for the rapid release, labeling, and detection of O-glycans present on a glycoconjugate, such as a glycoprotein, glycopeptide, peptidoglycan, or proteoglycan of interest. The compositions and methods allow the released O-glycans to be subjected to high-throughput analysis.

The invention relates to the technical field of glycosylation, and provides a DeepGP glycopeptide enrichment column and a method for efficiently enriching N glycopeptide and O glycopeptide. The preparation method of the DeepGP glycopeptide enrichment column comprises the following steps: uniformly mixing microcrystalline cellulose, cation exchange filler and anion exchange filler, and then packing the mixed filler into a column and compacting to obtain the DeepGP glycopeptide enrichment column. Compared with the enrichment effects of four enrichment columns on HET cell line glycopeptide samples, the DeepGP glycopeptide enrichment column prepared by the invention has the highest parallelism and enrichment efficiency in three times of parallel enrichment. Besides, 245 N glycopeptides can be identified in an IgG standard substance by utilizing a DeepGP column, and 4286 N glycopeptides and 749 O glycopeptides can be simultaneously identified in mouse plasma, so that the method is high in detection precision, high in sensitivity, good in repeatability and very wide in application range.

The invention relates to the technical field of health care products, and provides a glycopeptide composition and related products and application thereof, and the glycopeptide composition comprises the following raw materials: wheat germs, herba epimedii, fermented bean products, flaxseeds and radix bupleuri. The glycoside peptide composition formed by combining the raw materials has a synergistic effect in the aspect of cardiovascular system protection. All the components interact with one another to jointly achieve the effects of reducing serum total cholesterol and triglyceride, improving high-density lipoprotein cholesterol, resisting oxidation, improving vascular functions and the like, and the composition has a remarkable protection effect on a cardiovascular system. Besides, the activity is enriched into the five raw materials to the maximum extent, so that the method has remarkable advantages in the aspects of production process and raw material cost reduction, and is beneficial to improving the production efficiency, ensuring the product quality and reducing the operation cost.

The present invention relates to a cancer vaccine conjugate comprising a CRM197 carrier protein and an immunogenic peptide covalently linked to the residues C186 and 0201 of the CRM197 carrier by means of a linker. Suitable immunogenic peptides include glycopeptides, such as glycopeptides comprising a tumor associated carbohydrate antigen (TACA) (e.g., GalNAc, Galb (1, 3) GalNAc or Neu5Ac alpha (2-6) GalNAc). Cancer vaccine conjugates, methods of producing and uses thereof are provided.

The invention belongs to the technical field of drug detection, and particularly relates to a method for detecting lycium barbarum glycopeptide metabolites in rat plasma and tissues based on non-targeted metabonomics. According to the method, after rat lycium barbarum glycopeptides are administered through intragastric administration, blood, liver, intestine, lung and brain tissue samples after administration are collected. A UPLC-Q-TOF-MS (Ultra Performance Liquid Chromatography-Quadrupole Time of Flight Mass Spectrometry) analysis method is combined with a statistical analysis method comprising principal component analysis (PCA) and orthogonal-partial least squares discriminant analysis (OPLS-DA) to carry out in-vivo metabonomics analysis on the wolfberry glycopeptide. Then, based on a human metabolome database (HMDB), lycium barbarum glycopeptide differential metabolite is searched, so that the pharmacological action mechanism of the lycium barbarum glycopeptide is clarified from the perspective of metabolism, and a foundation is laid for deep research and development of the medicinal value of the lycium barbarum glycopeptide in future.

The invention discloses a stage targeting scallop adductor non-phosphorus water-retaining agent containing antifreeze glycopeptide and application, and relates to the technical field of food additives. The antifreeze glycopeptide-containing stage targeting scallop adductor phosphorus-free water-retaining agent consists of an agent A and an agent B, and does not contain phosphate additives, the agent A is permeable anti-freezing liquid and is prepared from the following components in percentage by mass: 5.0 percent to 9.0 percent of trehalose, 3.0 percent to 5.0 percent of sorbitol, 0.8 percent to 1.5 percent of sodium citrate, 0.4 percent to 1.0 percent of compound amino acid, 0.01 percent to 0.1 percent of high-activity anti-freezing glycopeptide, 0.01 percent to 0.05 percent of vitamin C, 0.01 percent to 0.05 percent of rosemary extract and the balance of water. According to the antifreeze glycopeptide-containing phase-targeting scallop adductor non-phosphorus water-retaining agent and the application thereof, ice crystal damage and protein denaturation are inhibited from the source, and cell-level protection of scallop adductors is realized. Through detection, the unfreezing loss rate of the treated scallop adductor is as low as about 2.12%, the cooking loss rate is as low as about 10.63%, and the water retention capacity is close to that of a commercially available phosphate water retention agent and is remarkably superior to that of an existing non-phosphorus water retention agent.

The invention discloses skipjack glycopeptide with anti-inflammatory activity and a preparation method and application thereof, and belongs to the technical field of deep processing of marine fish food. The technical problem to be solved is to improve the anti-inflammatory effect and the cell proliferation effect. The key point of the technical scheme is as follows: the molecular weight distribution of the skipjack glycopeptide with the anti-inflammatory activity comprises the following components in percentage by mass: 10.8-13.2% of components with the molecular weight smaller than or equal to 300 Da, and 86.8-89.2% of components with the molecular weight ranging from 300 Da to 2000 Da.