138 results about "Glycopeptide" patented technology

The preparation method comprises the following steps: by taking natural chitosan, epsilon-polylysine and polyphenol as raw materials, firstly, respectively preparing polyphenol modified chitosan and carboxyphenylboronic acid modified polylysine through a carbodiimide coupling method, then, mixing single-component solutions of the polyphenol modified chitosan and the carboxyphenylboronic acid modified polylysine, and then, preparing the antibacterial and antioxidant glycopeptide hydrogel based on the polyphenol. And the antibacterial and antioxidant glycopeptide hydrogel is obtained by virtue of the synergistic effect of the phenolic hydroxyl group-boric acid group dynamic bond and the amino group-carboxyl group. The hydrogel disclosed by the invention has broad-spectrum antibacterial property and glucose response oxidation resistance, has self-healing capability and strong wound adhesion, is excellent in biocompatibility and can be naturally degraded; and the process is simple, convenient and controllable, large-scale production is easy to realize, the hydrogel can be used as a wound dressing for treating chronic difficult-to-heal wounds such as diabetes mellitus in a targeting manner, and integrated nursing of antibiosis, antioxidation, wound adhesion and self-repairing is realized.

The application relates to a synthesis method of an anti-freezing glycopeptide polypeptide and belongs to the technical field of polypeptide drug synthesis. The method comprises the following steps: 2-CL-Resin is used as a carrier resin, under the condition of adding an activating agent, the carrier resin and alanine are coupled to obtain Fmoc-Ala-2-CL-Resin; according to the amino acid sequence of the anti-freezing glycopeptide, other amino acids are sequentially coupled through a solid-phase synthesis method; after a protecting group is removed and the carrier resin is cleaved, the anti-freezing glycopeptide crude peptide is obtained; and after purification, salt conversion and freeze-drying, the anti-freezing glycopeptide polypeptide is obtained. The method has the advantages of short synthesis period, low cost, easy post-treatment, few by-products, high product yield, facilitation of large-scale production of the anti-freezing glycopeptide, and considerable economic applicative value and wide application prospect.

The application discloses a glucagon peptide-1 and glucagon receptor dual-agonistic polypeptide and application thereof, and the polypeptide structure is shown in the following general formula (I). The dual-agonistic polypeptide has dual-agonistic activity on human GLP-1 and glucagon receptors. The GLP-1 / glucagon receptor dual-agonistic polypeptide can effectively reduce blood sugar and weight, and has obvious lipid-regulating effect. The GLP-1 / glucagon receptor dual-agonistic polypeptide is stable in vivo, and is suitable to be used as an active component of drugs for diabetes, obesity, hyperlipidemia and the like.

The invention discloses a high-throughput complete glycopeptide full-automatic online enrichment and separation analysis method which comprises the following steps: firstly, building a full-automatic online enrichment and separation analysis system capable of realizing synchronization of enrichment and separation analysis through switching of a ten-way valve; the full-automatic on-line enrichment and separation analysis system is adopted to enrich complete glycopeptides in a first-needle sample, then the full-automatic on-line enrichment and separation analysis system is adopted to enrich complete glycopeptides in a next-needle sample and separate and analyze complete glycopeptides in a previous-needle sample at the same time, and the above steps are repeated. The separation analysis of the complete glycopeptide in the last needle of sample is completed; the method has the advantages that the complete glycopeptide can be quickly, fully automatically and online enriched, separated and analyzed, the operation in the enrichment process is completely controllable, the problems of high sample loss rate, poor enrichment repeatability and low liquid chromatography separation degree can be greatly reduced, the time required by the complete glycopeptide enrichment and separation analysis steps is superposed, and the analysis accuracy is greatly improved. And the time required by the whole process can be obviously reduced.

The present invention relates to a cancer vaccine conjugate comprising a CRM197 carrier protein and an immunogenic peptide covalently linked to the residues C186 and 0201 of the CRM197 carrier by means of a linker. Suitable immunogenic peptides include glycopeptides, such as glycopeptides comprising a tumor associated carbohydrate antigen (TACA) (e.g., GalNAc, Galb (1, 3) GalNAc or Neu5Ac alpha (2-6) GalNAc). Cancer vaccine conjugates, methods of producing and uses thereof are provided.

The invention belongs to the technical field of drug detection, and particularly relates to a method for detecting lycium barbarum glycopeptide metabolites in rat plasma and tissues based on non-targeted metabonomics. According to the method, after rat lycium barbarum glycopeptides are administered through intragastric administration, blood, liver, intestine, lung and brain tissue samples after administration are collected. A UPLC-Q-TOF-MS (Ultra Performance Liquid Chromatography-Quadrupole Time of Flight Mass Spectrometry) analysis method is combined with a statistical analysis method comprising principal component analysis (PCA) and orthogonal-partial least squares discriminant analysis (OPLS-DA) to carry out in-vivo metabonomics analysis on the wolfberry glycopeptide. Then, based on a human metabolome database (HMDB), lycium barbarum glycopeptide differential metabolite is searched, so that the pharmacological action mechanism of the lycium barbarum glycopeptide is clarified from the perspective of metabolism, and a foundation is laid for deep research and development of the medicinal value of the lycium barbarum glycopeptide in future.

The invention discloses a stage targeting scallop adductor non-phosphorus water-retaining agent containing antifreeze glycopeptide and application, and relates to the technical field of food additives. The antifreeze glycopeptide-containing stage targeting scallop adductor phosphorus-free water-retaining agent consists of an agent A and an agent B, and does not contain phosphate additives, the agent A is permeable anti-freezing liquid and is prepared from the following components in percentage by mass: 5.0 percent to 9.0 percent of trehalose, 3.0 percent to 5.0 percent of sorbitol, 0.8 percent to 1.5 percent of sodium citrate, 0.4 percent to 1.0 percent of compound amino acid, 0.01 percent to 0.1 percent of high-activity anti-freezing glycopeptide, 0.01 percent to 0.05 percent of vitamin C, 0.01 percent to 0.05 percent of rosemary extract and the balance of water. According to the antifreeze glycopeptide-containing phase-targeting scallop adductor non-phosphorus water-retaining agent and the application thereof, ice crystal damage and protein denaturation are inhibited from the source, and cell-level protection of scallop adductors is realized. Through detection, the unfreezing loss rate of the treated scallop adductor is as low as about 2.12%, the cooking loss rate is as low as about 10.63%, and the water retention capacity is close to that of a commercially available phosphate water retention agent and is remarkably superior to that of an existing non-phosphorus water retention agent.

The invention discloses skipjack glycopeptide with anti-inflammatory activity and a preparation method and application thereof, and belongs to the technical field of deep processing of marine fish food. The technical problem to be solved is to improve the anti-inflammatory effect and the cell proliferation effect. The key point of the technical scheme is as follows: the molecular weight distribution of the skipjack glycopeptide with the anti-inflammatory activity comprises the following components in percentage by mass: 10.8-13.2% of components with the molecular weight smaller than or equal to 300 Da, and 86.8-89.2% of components with the molecular weight ranging from 300 Da to 2000 Da.

A FAP glycopeptide as represented by Formula (I), and a radionuclide marker thereof and the use thereof. The FAP glycopeptide is obtained by modifying a linker portion that forms a cyclic peptide on the basis of a polypeptide FAP-2286 structure, and further introducing mono- or di-saccharide molecules. After being marked with radionuclides, the glycopeptide shows superior tumor uptake and lower liver uptake compared to FAP-2286, resulting in better imaging outcomes, facilitating the development of candidate radionuclide pharmaceuticals with further research value, and contributing to the ultimate goal of developing FAP-targeted cyclic peptide radiopharmaceuticals with independent intellectual property rights. Drawing_references_to_be_translated

The present application relates to the technical field of microbial fermentation, in particular to a polyporus arctorus and a culture method and application thereof. A polyporus arctorus YH-DB-1 is screened in the present application, and a large amount of glycopeptide can be extracted from the mycelium of the strain by culturing the strain, the peptide content in the glycopeptide can reach more than 45%, and the present application solves the problem of low peptide yield in the existing polyporus arctorus glycopeptide.

Methods and kits for treating and / or preventing a cardiac disorder are disclosed. The methods can include administering an indole metabolite to a subject. The methods can also include administering a glycopeptide antibiotic to the subject.

The invention relates to a self-adjuvant based on a virus source glycopeptide and an application of the self-adjuvant in preparation of a vaccine, the self-adjuvant is a glycopeptide fragment based on a varicella-zoster virus (VZV) source, and the self-adjuvant is a B cell epitope structural domain V50 glycopeptide (from 50 valine to 135 isoleucine, V50-I135) in glycoprotein E. The invention further relates to a preparation method of the self-adjuvant based on the virus source glycopeptide and application of the self-adjuvant based on the virus source glycopeptide in preparation of the vaccine. The V50 glycopeptide and the novel coronavirus RBD are subjected to fusion expression and then are coupled to the surfaces of the nanoparticles based on ferritin to form the self-adjuvant vaccine, so that the levels of RBD specific antibodies and pseudovirus neutralizing antibodies induced by the RBD nanoparticle vaccine can be remarkably enhanced, and the V50 glycopeptide has a synergistic effect in B cell response and T cell response of systemic systemic immunity and mucosal immunity at the same time. The immune protection effect of the novel coronavirus RBD nanoparticle vaccine is obviously improved.

The present invention relates to a monoclonal antibody reacting with a glycopeptide and use thereof. The present invention provides a monoclonal antibody which is an epitope of both a fucose moiety and an amino acid of a peptide moiety. The antibody of the present invention is a monoclonal antibody reacting with a glycopeptide containing a core fucose and an amino acid of 4 or more residues continuously from an asparagine of an additional sugar chain toward a C-terminal side, the antibody being an epitope of both the core fucose of the glycopeptide and an amino acid of the glycopeptide which is 3 or more residues from the asparagine of the additional sugar chain toward the C-terminal side.

The invention belongs to the technical field of deep processing of lycium barbarum, and particularly relates to a lycium barbarum polysaccharide molecular weight grading co-production method and an anti-tumor product. The molecular weight grading co-production method of lycium barbarum polysaccharide comprises the following steps: crushing dried lycium barbarum, carrying out subcritical extraction, collecting extraction residues to obtain lycium barbarum residues, adding water, pulping, and separating to obtain lycium barbarum juice and lycium barbarum seeds. And mixing the lycium barbarum seed washing water with the lycium barbarum juice to obtain the lycium barbarum pulp. Performing enzymolysis on the Chinese wolfberry pulp to obtain Chinese wolfberry pulp enzymatic hydrolysate; and centrifuging the wolfberry pulp enzymatic hydrolysate to obtain the wolfberry pulp. Performing ceramic membrane filtration, 100kDa ultrafiltration, 30kDa ultrafiltration, 10kDa ultrafiltration and 3kDa ultrafiltration on the Chinese wolfberry pulp, and respectively collecting filtrate and interception liquid to obtain Chinese wolfberry polysaccharide and Chinese wolfberry glycopeptide. And fermenting the 3KDa ultrafiltrate to obtain the Chinese wolfberry wine and the Chinese wolfberry brandy. According to the method, the lycium barbarum polysaccharide separation efficiency is high, the process synergy is high, and a new thought is provided for development of the lycium barbarum industry.

The invention belongs to the technical field of biological medicine, and discloses ovomucoprotein glycopeptide and application thereof. According to the application of the ovomucoprotein glycopeptide in preparing the medicine for preventing or treating the alcohol-induced acute gastric mucosal lesion, the percentage of the gastric mucosal lesion area can be reduced to 1.0% or below when the ovomucoprotein glycopeptide is used for protection. The invention creatively discovers and verifies that the glycopeptide derived from ovomucin has a remarkable protective effect on acute gastric mucosal lesion induced by alcohol. According to the discovery of the application, the application field of the natural active ingredient ovomucin is definitely expanded to the protection of acute gastric mucosal lesion for the first time, the technical problem that efficient and natural gastric mucosal protection ingredients are lacked in the prior art is solved, and a brand-new material basis and a clear direction are provided for developing a novel gastric mucosal protective agent.

1. The name of the design product: packaging box (Ganoderma spore polysaccharide peptide tablets). 2. The use of the design product: for product outer packaging. 3. The design points of the design product: in the shape of the product, pattern and their combination. 4. The picture or photo that best indicates the design points: perspective view.

The invention provides a biomarker for diagnosing primary biliary cholangitis and application of the biomarker, and belongs to the technical field of biomarkers. The biomarker disclosed by the invention comprises serum protein and / or N-glycopeptide. According to the method, the improvement of PBC diagnosis is taken as a starting point, the serum protein and the N-glycopeptide are taken as screening templates, a more accurate non-invasive prediction means for differential diagnosis of PBC is provided, and a theoretical basis is provided for rapid and accurate diagnosis of PBC.

This invention belongs to the technical field of natural product active ingredients, specifically relating to a glycopeptide LBGP-I-b derived from wolfberry and its uses. The glycopeptide LBGP-I-b has an average molecular weight of 700–1000 Da, a polypeptide content of 70%–80%, and a polysaccharide content of 10%–20%. The sugar chains are composed of glucose residues, and the sugar chains are linked to the peptide chains via O-glycopeptide bonds. The glycopeptide has functions such as regulating lipid metabolism, lowering blood lipid levels, and improving liver function. The glycopeptide of this invention has a clear source and stable properties, and has good application prospects in the prevention and treatment of metabolic diseases such as metabolic-related fatty liver disease.

The invention discloses hypoglycemic peptide sheep milk powder and a preparation method thereof, and belongs to the technical field of enzyme engineering. According to the preparation method, sheep milk is taken as a raw material, the inhibition rates of different proteases on dipeptidyl peptidase IV (DPP-IV) are compared after the sheep milk is subjected to enzymolysis by different proteases, the optimal compound protease is obtained, then the hypoglycemic peptide sheep milk is obtained through hydrolysis of the compound protease, the hypoglycemic peptide sheep milk has DPP-IV inhibition activity, and the sheep milk powder with the hypoglycemic activity is further obtained through spray drying. The DPP-IV inhibition rate is 69.44% + / -2.66%-87.45% + / -1.25%, and the compound can be used for assisting in reducing blood sugar of consumers and reducing the dosage of hypoglycemic peptides so as to reduce side effects. The hypoglycemic peptides in the enzymolysis sheep milk are separated, purified and identified through ultrafiltration, preparative chromatography and HPLC-MS / MS, 12 hypoglycemic peptides from sheep milk protein are obtained, and theoretical and technical supports are provided for development of hypoglycemic assisting dairy products.

The invention relates to a PKM2-based nano glycopeptide activator and application thereof.The nano glycopeptide activator comprises three functional units, namely a PKM2 targeted activation unit R3, a self-assembly unit R2 and a response unit R1, and the chemical structure of the nano glycopeptide activator is shown in the formula (I). After administration, the nano glycopeptide activator can be enriched to a tumor site through targeting of sugar receptor transporter protein or an EPR effect; after entering tumor cells, the polypeptide is subjected to in-situ fibrosis deformation under the hydrolysis action of glycohydrolase, so that specific targeting and retention are realized; the nano-fiber with the PKM2 targeting activation unit can promote conversion of dimer PKM2 into tetramer PKM2, inhibit nucleation of PKM2, and prevent the DNA damage repair process of tumor cells.

The invention belongs to the field of biosensing and biological interface engineering, and particularly relates to a double-enhanced bionic glycopeptide electrochemical biosensor based on anti-pollution and anti-enzymolysis stability and a preparation method of the double-enhanced bionic glycopeptide electrochemical biosensor. The invention is inspired by glycoprotein in vascular endothelial cell glycocalyx, a novel glycopeptide material capable of enhancing biological pollution resistance and protease hydrolysis resistance stability is constructed by performing glycosylation modification at the C terminal of a polypeptide sequence, and the novel glycopeptide material is applied to a complex biological medium and has remarkable performance superior to that of non-glycosylated peptide.

The invention belongs to the technical field of food-borne functional factors, and particularly relates to a bioactive glycopeptide as well as a preparation method and application thereof. According to the application, casein glycomacropeptide is taken as a substrate, specific protease is adopted for carrying out directional enzymolysis reaction on the casein glycomacropeptide, and after primary separation and purification by hydrophilic interaction chromatography and secondary purification by cation exchange chromatography are carried out, the bioactive glycopeptide with the molecular weight of less than 1,500 Da and more than 80% of components and the random coil secondary structure content of more than 35% is obtained. A system performance test shows that in an in-vitro cell experiment, the bioactive glycopeptide prepared by the embodiment of the invention can remarkably improve the proliferation rate of preosteoblasts and lay a sufficient cell quantity foundation for bone cell generation; meanwhile, the activity of an osteogenic early differentiation key marker ALP is effectively up-regulated, and the differentiation process of preosteoblasts towards the osteoblast direction is accelerated; in addition, the expression quantity of osteogenesis-related regulatory protein calcium-sensitive receptors is remarkably increased, and the function improvement of cells to mature bone cells is further promoted.

The preparation method comprises the following steps: (1) compounding hermetia illucens larvae, whole tenebrio molitor and euphausia superba according to a mass ratio of (3-5): (2-4): 1, carrying out enzymolysis, and reacting with a yeast cell wall extract to form a glycopeptide compound; (2) coating the glycopeptide compound with a mannan oligosaccharide and chitosan solution to form microcapsules, and controlling the release rate of the microcapsules in simulated gastric juice to be lower than 30% and the release rate of the microcapsules in simulated intestinal juice to be higher than 80%; (3) co-fermenting the microcapsules, clostridium butyricum and pediococcus acidilactici, and adsorbing fermentation liquor on the porous starch; (4) performing dry mixing on the functional compound, the fermentation compound and the base material, and performing low-temperature puffing; and (5) sequentially spraying a vitamin D3 grease layer, a pectin-whey protein protective layer and a solution containing TGF-beta2 on the surfaces of the particles. Through cooperation of multiple processes, the immune function and intestinal health of pets are remarkably improved, and the product is good in stability and palatability.

The invention provides multivalent glycopeptide as well as a preparation method and application thereof, and belongs to the field of polypeptide drugs. The structure of the multivalent glycopeptide is shown as a formula I. A polycysteine peptide chain is used as a main chain, glycosyl sulfinate is used as a raw material to carry out multivalent glycosylation modification, the novel multivalent glycopeptide is obtained, the multivalent glycopeptide has multiple potential application values, and when acetylamino galactose is used for multivalent glycosylation modification, the multivalent glycopeptide can be used for preparing the novel multivalent glycopeptide. The obtained acetamino galactose multivalent glycopeptide has excellent liver targeting ability which is higher than that of traditional commercial trisaccharide of the Alnalym company, and the design can meet the requirements of biomedical research and drug development on multivalent glycopeptide. Formula I

The invention discloses a yak skin oligopeptide, a preparation method and application thereof, and a functional food or medicine, and belongs to the technical field of bioactive peptides, the yak skin oligopeptide comprises at least one of RDIFL, FRVP, RDILF and FDLRF oligopeptide, compared with other hypoglycemic peptides, the molecular weight is smaller, the yak skin oligopeptide can be directly absorbed by intestinal tracts, and the effect of delaying blood sugar rise is achieved. The oligopeptide RDIFL, the oligopeptide FRVP, the oligopeptide RDILF and the oligopeptide FDLRF have excellent inhibitory activity on alpha-glucosidase, the semi-inhibitory concentrations of the oligopeptide RDIFL, the oligopeptide FRVP, the oligopeptide RDILF and the oligopeptide FDLRF can reach 10.107 mmol / L, 13.063 mmol / L, 8.653 mmol / L and 12.369 mmol / L respectively, digestion and absorption of carbohydrates can be effectively delayed,

Endoglycosidase-assisted peptide mapping workflow systems for mass spectrometry (MS) characterization of non-consensus N-glycosylation in monoclonal antibodies (mAbs) are disclosed. The feasibility of the workflow was demonstrated by an atypical glycosite located within an NPNNXN (SEQ ID NO: 1) sequence in a 25-residue tryptic peptide. With the aids of endoglycosidase treatment, the resulting truncated glycan structures improved peptide ionization efficiency in MS and hence facilitated reliable quantitation of glycosite occupancy. The remaining mono- / di-saccharides served as a large mass tag allowing differentiation between the glycopeptide and deamidated peptide, thus allowing for database searching for glycosite localization and automation of the data processing workflow. This workflow offers an efficient solution for characterizing non-consensus N-glycosylation for the development of therapeutic mAbs.

The application discloses a kind of bifunctional magnetic nano material and its preparation method and application.The nano material described in the application is Fe3O4@ZrO2@TiO2@mSiO2-L-Cys, with spherical structure;The material includes successively from inside to outside: the inner core is the magnetic nanoparticle of ferriferrous oxide;Intermediate layer is zirconium dioxide and titanium dioxide composite metal oxide layer;Sub-outer layer is mesoporous silica layer;The outermost layer is the L-cysteine molecular layer grafted by zirconium-sulfur chemical bond;The preparation method described in the application adopts solvothermal method to synthesize magnetic core, functional layer is coated by hydrothermal reaction, co-condensation method constructs mesoporous shell, and hydrophilic group is introduced by chemical grafting.The bifunctional magnetic mesoporous nano material prepared in the application is applied to the effective enrichment of glycopeptide and phosphopeptide in standard protease solution mixture, and the detection sensitivity of phosphopeptide and glycopeptide reaches 1 fmol / μL, which has wide prospect in glycopeptide and phosphopeptide proteomics analysis research.

The present application belongs to the technical field of polypeptide processing, and particularly relates to a preparation method of protein peptide capable of improving flavor and function; the method uses silver carp as raw material, uses food-grade protease, and obtains full fish protein peptide through moderate enzymolysis; under mild conditions, nitrogen is used to replace air to perform glycosylation reaction, which can effectively improve the flavor and antioxidant function of the full fish protein peptide; compared with the original full fish protein peptide and the full fish protein glycopeptide prepared by using air for glycosylation reaction, the full fish protein glycopeptide prepared by the method has lighter fishy smell and bitterness, can effectively improve the acceptance of the public, has strong antioxidant function and good storage stability, and can be widely applied in special food and nutritional food.

The invention provides valerian ether hydroformylated glycine active molecules and a preparation method and application thereof, an active carrier and preparation and application thereof, and belongs to the technical field of natural material hybridization. According to the preparation method, valerian ether hydroformylated glycine active molecules are obtained through one-step coupling reaction of natural product glycylglycine and natural valerian ether aldehyde, a large number of polar groups on the surface of natural oxyhydrogen stone powder can be effectively shielded in a multi-level mode, the surface polarity of the natural oxyhydrogen stone powder is reduced, and the oil absorption value of the natural magnesium-based powder is reduced; meanwhile, after the valerian ether hydroformylated glycine peptide active molecule is used as a low-polarity multi-component natural product activation molecule to modify the natural magnesium oxyhydroxide powder, the processing cost can be greatly saved, and the dispersity of a natural magnesium oxyhydroxide-based activation carrier in a low-polarity energy base material can be effectively improved, so that the activity of the valerian ether hydroformylated glycine peptide active molecule is improved, and the service life of the valerian ether hydroformylated glycine peptide active molecule is prolonged. The method has a good promotion effect on high value and functional improvement of the natural magnesium-based mineral powder, and can endow the activating carrier with multiple functions.