206 results about "Glycopeptide" patented technology

The invention relates to the technical field of bioanalytical chemistry, in particular to a method for simultaneously enriching and separating glycopeptides and phosphopeptides by using a bionic hydrophilic adsorbent, which specifically comprises the following steps: step 1, preparing a polyether-ether-ketone (PEEK) framework by using a fused deposition modeling method (FDM); 2, soaking the obtained PEEK framework in an aqueous solution containing dopamine hydrochloride and tris (hydroxymethyl) aminomethane, and carrying out a reaction; step 3, then, adding a mixed solution containing hyaluronic acid methacrylate, vinyl phosphoric acid, N, N-dimethyl bisacrylamide and phenyl-2, 4, 6-trimethylbenzoyl lithium phosphate into the PEEK framework; in the invention, in the aspect of material preparation, a fused deposition modeling method (FDM) is adopted to prepare a polyether-ether-ketone (PEEK) frame, and the material has good mechanical properties and chemical stability and can bear a series of subsequent complex treatment processes without being damaged.

The invention discloses cubilose sialic acid glycan peptide capable of improving lung inflammation and application of the cubilose sialic acid glycan peptide. The ratio of fucose modified sugar chains in the cubilose sialic acid glycan peptide is 30-60%, the ratio of sialic acid modified sugar chains in the cubilose sialic acid glycan peptide is 20-40%, the core structure of the fucose type is (HexNAc) 3 (Man) 3 (Fuc) 1, the core structure of the sialic acid type is (HexNAc) 3 (Man) 3 (Gal) 1 (Fuc) 1 (NeuAc) 1, the neutral sugar dissolution rate of the cubilose sialic acid glycan peptide is high, and the glycopeptide shown in SEQ ID NO 2 has a good inflammation improving effect.

The preparation method comprises the following steps: by taking natural chitosan, epsilon-polylysine and polyphenol as raw materials, firstly, respectively preparing polyphenol modified chitosan and carboxyphenylboronic acid modified polylysine through a carbodiimide coupling method, then, mixing single-component solutions of the polyphenol modified chitosan and the carboxyphenylboronic acid modified polylysine, and then, preparing the antibacterial and antioxidant glycopeptide hydrogel based on the polyphenol. And the antibacterial and antioxidant glycopeptide hydrogel is obtained by virtue of the synergistic effect of the phenolic hydroxyl group-boric acid group dynamic bond and the amino group-carboxyl group. The hydrogel disclosed by the invention has broad-spectrum antibacterial property and glucose response oxidation resistance, has self-healing capability and strong wound adhesion, is excellent in biocompatibility and can be naturally degraded; and the process is simple, convenient and controllable, large-scale production is easy to realize, the hydrogel can be used as a wound dressing for treating chronic difficult-to-heal wounds such as diabetes mellitus in a targeting manner, and integrated nursing of antibiosis, antioxidation, wound adhesion and self-repairing is realized.

The invention belongs to the field of cell culture, and relates to an MRC-5 cell serum-free medium with definite components and application thereof. Nutrient substances of the serum-free culture medium comprise amino acids, vitamins, inorganic salts, trace elements, proteins, other substances and supplements. According to the MRC-5 cell serum-free culture medium, the concentration of the amino acid, the inorganic salt, the vitamin and the serum substitute is adjusted, and meanwhile, the Chinese yam glycopeptide is added, so that adherent growth of MRC-5 cells can be effectively promoted, and the technical problem that a new variety of MRC-5 cell serum-free culture medium is lacked in the prior art is solved; the requirements of nutrient substances, carbon sources and genetic material nutrient substances required by MRC-5 cell in-vitro culture can be met.

The application relates to a synthesis method of an anti-freezing glycopeptide polypeptide and belongs to the technical field of polypeptide drug synthesis. The method comprises the following steps: 2-CL-Resin is used as a carrier resin, under the condition of adding an activating agent, the carrier resin and alanine are coupled to obtain Fmoc-Ala-2-CL-Resin; according to the amino acid sequence of the anti-freezing glycopeptide, other amino acids are sequentially coupled through a solid-phase synthesis method; after a protecting group is removed and the carrier resin is cleaved, the anti-freezing glycopeptide crude peptide is obtained; and after purification, salt conversion and freeze-drying, the anti-freezing glycopeptide polypeptide is obtained. The method has the advantages of short synthesis period, low cost, easy post-treatment, few by-products, high product yield, facilitation of large-scale production of the anti-freezing glycopeptide, and considerable economic applicative value and wide application prospect.

The application discloses a glucagon peptide-1 and glucagon receptor dual-agonistic polypeptide and application thereof, and the polypeptide structure is shown in the following general formula (I). The dual-agonistic polypeptide has dual-agonistic activity on human GLP-1 and glucagon receptors. The GLP-1 / glucagon receptor dual-agonistic polypeptide can effectively reduce blood sugar and weight, and has obvious lipid-regulating effect. The GLP-1 / glucagon receptor dual-agonistic polypeptide is stable in vivo, and is suitable to be used as an active component of drugs for diabetes, obesity, hyperlipidemia and the like.

The invention discloses a high-throughput complete glycopeptide full-automatic online enrichment and separation analysis method which comprises the following steps: firstly, building a full-automatic online enrichment and separation analysis system capable of realizing synchronization of enrichment and separation analysis through switching of a ten-way valve; the full-automatic on-line enrichment and separation analysis system is adopted to enrich complete glycopeptides in a first-needle sample, then the full-automatic on-line enrichment and separation analysis system is adopted to enrich complete glycopeptides in a next-needle sample and separate and analyze complete glycopeptides in a previous-needle sample at the same time, and the above steps are repeated. The separation analysis of the complete glycopeptide in the last needle of sample is completed; the method has the advantages that the complete glycopeptide can be quickly, fully automatically and online enriched, separated and analyzed, the operation in the enrichment process is completely controllable, the problems of high sample loss rate, poor enrichment repeatability and low liquid chromatography separation degree can be greatly reduced, the time required by the complete glycopeptide enrichment and separation analysis steps is superposed, and the analysis accuracy is greatly improved. And the time required by the whole process can be obviously reduced.

The invention belongs to the technical field of biological medicines, and particularly relates to a cyclic ester peptide compound, a producing strain thereof, a preparation method and application of the cyclic ester peptide compound in preparation of antibacterial medicines. The invention provides a cyclic ester peptide compound as shown in a formula I, a stereoisomer, a tautomer and an isotope derivative of the cyclic ester peptide compound, or a pharmaceutically acceptable salt of the cyclic ester peptide compound, a producing strain Streptomyces alboflavus sp.NX-12 of the cyclic ester peptide compound, and fermentation, separation and purification methods of the cyclic ester peptide compound and the stereoisomer, the tautomer and the isotope derivative of the cyclic ester peptide compound. The cyclic ester peptide compound has strong inhibitory activity on a variety of gram-positive bacteria, can be used for preparing antibacterial drugs, shows application potential in treatment of infection and other related diseases, and compared with the existing similar gram-positive bacterium-resistant glycopeptide antibiotic vancomycin, the cyclic ester peptide compound has the advantages that the cyclic ester peptide compound can be used for preparing the antibacterial drugs; the cyclic ester peptide compound disclosed by the invention has an equivalent antibacterial effect. The preparation method of the cyclic ester peptide compound is simple in process, short in time consumption, capable of greatly reducing the medicine cost and suitable for large-scale production. # imgabs0 #

The present disclosure provides methods, compositions, and kits for the rapid release, labeling, and detection of O-glycans present on a glycoconjugate, such as a glycoprotein, glycopeptide, peptidoglycan, or proteoglycan of interest. The compositions and methods allow the released O-glycans to be subjected to high-throughput analysis.

The present invention relates to a cancer vaccine conjugate comprising a CRM197 carrier protein and an immunogenic peptide covalently linked to the residues C186 and 0201 of the CRM197 carrier by means of a linker. Suitable immunogenic peptides include glycopeptides, such as glycopeptides comprising a tumor associated carbohydrate antigen (TACA) (e.g., GalNAc, Galb (1, 3) GalNAc or Neu5Ac alpha (2-6) GalNAc). Cancer vaccine conjugates, methods of producing and uses thereof are provided.

The invention belongs to the technical field of drug detection, and particularly relates to a method for detecting lycium barbarum glycopeptide metabolites in rat plasma and tissues based on non-targeted metabonomics. According to the method, after rat lycium barbarum glycopeptides are administered through intragastric administration, blood, liver, intestine, lung and brain tissue samples after administration are collected. A UPLC-Q-TOF-MS (Ultra Performance Liquid Chromatography-Quadrupole Time of Flight Mass Spectrometry) analysis method is combined with a statistical analysis method comprising principal component analysis (PCA) and orthogonal-partial least squares discriminant analysis (OPLS-DA) to carry out in-vivo metabonomics analysis on the wolfberry glycopeptide. Then, based on a human metabolome database (HMDB), lycium barbarum glycopeptide differential metabolite is searched, so that the pharmacological action mechanism of the lycium barbarum glycopeptide is clarified from the perspective of metabolism, and a foundation is laid for deep research and development of the medicinal value of the lycium barbarum glycopeptide in future.

The invention discloses a stage targeting scallop adductor non-phosphorus water-retaining agent containing antifreeze glycopeptide and application, and relates to the technical field of food additives. The antifreeze glycopeptide-containing stage targeting scallop adductor phosphorus-free water-retaining agent consists of an agent A and an agent B, and does not contain phosphate additives, the agent A is permeable anti-freezing liquid and is prepared from the following components in percentage by mass: 5.0 percent to 9.0 percent of trehalose, 3.0 percent to 5.0 percent of sorbitol, 0.8 percent to 1.5 percent of sodium citrate, 0.4 percent to 1.0 percent of compound amino acid, 0.01 percent to 0.1 percent of high-activity anti-freezing glycopeptide, 0.01 percent to 0.05 percent of vitamin C, 0.01 percent to 0.05 percent of rosemary extract and the balance of water. According to the antifreeze glycopeptide-containing phase-targeting scallop adductor non-phosphorus water-retaining agent and the application thereof, ice crystal damage and protein denaturation are inhibited from the source, and cell-level protection of scallop adductors is realized. Through detection, the unfreezing loss rate of the treated scallop adductor is as low as about 2.12%, the cooking loss rate is as low as about 10.63%, and the water retention capacity is close to that of a commercially available phosphate water retention agent and is remarkably superior to that of an existing non-phosphorus water retention agent.

The invention discloses skipjack glycopeptide with anti-inflammatory activity and a preparation method and application thereof, and belongs to the technical field of deep processing of marine fish food. The technical problem to be solved is to improve the anti-inflammatory effect and the cell proliferation effect. The key point of the technical scheme is as follows: the molecular weight distribution of the skipjack glycopeptide with the anti-inflammatory activity comprises the following components in percentage by mass: 10.8-13.2% of components with the molecular weight smaller than or equal to 300 Da, and 86.8-89.2% of components with the molecular weight ranging from 300 Da to 2000 Da.

A FAP glycopeptide as represented by Formula (I), and a radionuclide marker thereof and the use thereof. The FAP glycopeptide is obtained by modifying a linker portion that forms a cyclic peptide on the basis of a polypeptide FAP-2286 structure, and further introducing mono- or di-saccharide molecules. After being marked with radionuclides, the glycopeptide shows superior tumor uptake and lower liver uptake compared to FAP-2286, resulting in better imaging outcomes, facilitating the development of candidate radionuclide pharmaceuticals with further research value, and contributing to the ultimate goal of developing FAP-targeted cyclic peptide radiopharmaceuticals with independent intellectual property rights. Drawing_references_to_be_translated

The present application relates to the technical field of microbial fermentation, in particular to a polyporus arctorus and a culture method and application thereof. A polyporus arctorus YH-DB-1 is screened in the present application, and a large amount of glycopeptide can be extracted from the mycelium of the strain by culturing the strain, the peptide content in the glycopeptide can reach more than 45%, and the present application solves the problem of low peptide yield in the existing polyporus arctorus glycopeptide.

Methods and kits for treating and / or preventing a cardiac disorder are disclosed. The methods can include administering an indole metabolite to a subject. The methods can also include administering a glycopeptide antibiotic to the subject.

The invention relates to a self-adjuvant based on a virus source glycopeptide and an application of the self-adjuvant in preparation of a vaccine, the self-adjuvant is a glycopeptide fragment based on a varicella-zoster virus (VZV) source, and the self-adjuvant is a B cell epitope structural domain V50 glycopeptide (from 50 valine to 135 isoleucine, V50-I135) in glycoprotein E. The invention further relates to a preparation method of the self-adjuvant based on the virus source glycopeptide and application of the self-adjuvant based on the virus source glycopeptide in preparation of the vaccine. The V50 glycopeptide and the novel coronavirus RBD are subjected to fusion expression and then are coupled to the surfaces of the nanoparticles based on ferritin to form the self-adjuvant vaccine, so that the levels of RBD specific antibodies and pseudovirus neutralizing antibodies induced by the RBD nanoparticle vaccine can be remarkably enhanced, and the V50 glycopeptide has a synergistic effect in B cell response and T cell response of systemic systemic immunity and mucosal immunity at the same time. The immune protection effect of the novel coronavirus RBD nanoparticle vaccine is obviously improved.

The present invention relates to a monoclonal antibody reacting with a glycopeptide and use thereof. The present invention provides a monoclonal antibody which is an epitope of both a fucose moiety and an amino acid of a peptide moiety. The antibody of the present invention is a monoclonal antibody reacting with a glycopeptide containing a core fucose and an amino acid of 4 or more residues continuously from an asparagine of an additional sugar chain toward a C-terminal side, the antibody being an epitope of both the core fucose of the glycopeptide and an amino acid of the glycopeptide which is 3 or more residues from the asparagine of the additional sugar chain toward the C-terminal side.

The invention belongs to the technical field of deep processing of lycium barbarum, and particularly relates to a lycium barbarum polysaccharide molecular weight grading co-production method and an anti-tumor product. The molecular weight grading co-production method of lycium barbarum polysaccharide comprises the following steps: crushing dried lycium barbarum, carrying out subcritical extraction, collecting extraction residues to obtain lycium barbarum residues, adding water, pulping, and separating to obtain lycium barbarum juice and lycium barbarum seeds. And mixing the lycium barbarum seed washing water with the lycium barbarum juice to obtain the lycium barbarum pulp. Performing enzymolysis on the Chinese wolfberry pulp to obtain Chinese wolfberry pulp enzymatic hydrolysate; and centrifuging the wolfberry pulp enzymatic hydrolysate to obtain the wolfberry pulp. Performing ceramic membrane filtration, 100kDa ultrafiltration, 30kDa ultrafiltration, 10kDa ultrafiltration and 3kDa ultrafiltration on the Chinese wolfberry pulp, and respectively collecting filtrate and interception liquid to obtain Chinese wolfberry polysaccharide and Chinese wolfberry glycopeptide. And fermenting the 3KDa ultrafiltrate to obtain the Chinese wolfberry wine and the Chinese wolfberry brandy. According to the method, the lycium barbarum polysaccharide separation efficiency is high, the process synergy is high, and a new thought is provided for development of the lycium barbarum industry.

The invention provides a method for preparing micromolecular glucosamine peptide by utilizing leftover materials of aquatic products. The method comprises the following three steps: firstly, treating raw materials through alkaline hydrolysis, and removing impurities; secondly, chitinase, deacetylase and collagenase are added at a certain temperature for enzymolysis, and the raw materials are converted into micromolecular collagen peptide; and finally, carrying out activated carbon adsorption, ultrafiltration membrane filtration and heating evaporation concentration to obtain a high-purity glucosamine peptide solution. The method is efficient, environment-friendly and low in cost, waste generated in the aquatic product processing process can be fully utilized and converted into micromolecular amino glycopeptides with commercial value, cyclic utilization of resources is promoted, and the method has high sustainable development significance and wide industrial application prospects.

The invention belongs to the technical field of biological medicine, and discloses ovomucoprotein glycopeptide and application thereof. According to the application of the ovomucoprotein glycopeptide in preparing the medicine for preventing or treating the alcohol-induced acute gastric mucosal lesion, the percentage of the gastric mucosal lesion area can be reduced to 1.0% or below when the ovomucoprotein glycopeptide is used for protection. The invention creatively discovers and verifies that the glycopeptide derived from ovomucin has a remarkable protective effect on acute gastric mucosal lesion induced by alcohol. According to the discovery of the application, the application field of the natural active ingredient ovomucin is definitely expanded to the protection of acute gastric mucosal lesion for the first time, the technical problem that efficient and natural gastric mucosal protection ingredients are lacked in the prior art is solved, and a brand-new material basis and a clear direction are provided for developing a novel gastric mucosal protective agent.

The invention provides a fusion protein based on a GLP-1 analogue, a T cell therapeutic drug and an application of the fusion protein and the T cell therapeutic drug in tumor resistance, and relates to the field of preparation of antibodies and cell therapeutic drugs. The fusion protein based on the GLP-1 analogue is obtained by fusing a T cell activation antibody and the GLP-1 analogue, the T cell activation antibody is a BiTE antibody, the BiTE antibody is a CD3 / EpCAM fusion antibody, and the GLP-1 analogue is dulaglycopeptide; the CD3 / EpCAM fusion antibody is composed of a light chain and a heavy chain of a CD3 antibody and a light chain and a heavy chain of an EpCAM antibody, wherein the heavy chain of the CD3 antibody and the heavy chain of the EpCAM antibody are connected through a peptide linker; the C end of the dulatide is fused with the N end of the light chain of the CD3 antibody, and the middle is connected with the N end through a peptide joint; the obtained fusion protein based on the GLP-1 analogue can be used for preparing anti-tumor drugs and T cell treatment drugs, and has the effects of reducing toxicity and enhancing efficacy.

1. The name of the design product: packaging box (Ganoderma spore polysaccharide peptide tablets). 2. The use of the design product: for product outer packaging. 3. The design points of the design product: in the shape of the product, pattern and their combination. 4. The picture or photo that best indicates the design points: perspective view.

The invention provides a biomarker for diagnosing primary biliary cholangitis and application of the biomarker, and belongs to the technical field of biomarkers. The biomarker disclosed by the invention comprises serum protein and / or N-glycopeptide. According to the method, the improvement of PBC diagnosis is taken as a starting point, the serum protein and the N-glycopeptide are taken as screening templates, a more accurate non-invasive prediction means for differential diagnosis of PBC is provided, and a theoretical basis is provided for rapid and accurate diagnosis of PBC.

The invention relates to the field of nano materials, in particular to a DNA tetrahedron-based magnetic nano material as well as a preparation method and application thereof. According to the magnetic nano material Fe3O4 (at) DOPA / PEI (at) Au (at) DNA TET-Trypsin based on the DNA tetrahedron, regular distribution of trypsin on the surface of the material is achieved by utilizing the structural characteristics of the DNA tetrahedron, and glycopeptide is enriched by utilizing rich hydrophilic groups on the surface; the preparation method comprises the following steps: introducing PEI on the surface of magnetic Fe3O4, and synthesizing a polymer Fe3O4 (at) DOPA / PEI with rich amino groups on the surface through Schiff base reaction; then, gold nanoparticles are deposited on the polymer through an AuNPs solution, and DNA tetrahedrons with-SH at the tops are successfully attached to the gold nanoparticles through Au-S bonds; and finally, synthesizing the magnetic nano material through amination reaction between amido and carboxyl. Compared with a traditional enrichment material, the magnetic nano material disclosed by the invention has excellent digestion and enrichment performance, and not only can realize rapid digestion of protein but also can perform specific enrichment on glycopeptide within a very short reaction time.

This invention belongs to the technical field of natural product active ingredients, specifically relating to a glycopeptide LBGP-I-b derived from wolfberry and its uses. The glycopeptide LBGP-I-b has an average molecular weight of 700–1000 Da, a polypeptide content of 70%–80%, and a polysaccharide content of 10%–20%. The sugar chains are composed of glucose residues, and the sugar chains are linked to the peptide chains via O-glycopeptide bonds. The glycopeptide has functions such as regulating lipid metabolism, lowering blood lipid levels, and improving liver function. The glycopeptide of this invention has a clear source and stable properties, and has good application prospects in the prevention and treatment of metabolic diseases such as metabolic-related fatty liver disease.

The invention belongs to the technical field of medicines, and particularly relates to application of lycium barbarum glycopeptide in preparation of a medicine for preventing or treating senile ossification. The experimental results show that the wolfberry glycopeptide can improve the posterior limb movement ability and the bone trabecula condition of old mice by increasing the femoral hardness, relieving the bone tissue calcium loss condition, improving the bone formation rate, promoting osteoblasts and inhibiting osteoclasts, so that the effect of treating bone aging is achieved. As a traditional Chinese medicinal material extraction product, the lycium barbarum glycopeptide can be effectively used for preventing and treating senile ossification, has the prospect of being developed into the medicine for preventing and treating senile ossification, provides more treatment choices for clinically preventing or treating senile ossification, and has important social benefits and economic values.

The invention discloses hypoglycemic peptide sheep milk powder and a preparation method thereof, and belongs to the technical field of enzyme engineering. According to the preparation method, sheep milk is taken as a raw material, the inhibition rates of different proteases on dipeptidyl peptidase IV (DPP-IV) are compared after the sheep milk is subjected to enzymolysis by different proteases, the optimal compound protease is obtained, then the hypoglycemic peptide sheep milk is obtained through hydrolysis of the compound protease, the hypoglycemic peptide sheep milk has DPP-IV inhibition activity, and the sheep milk powder with the hypoglycemic activity is further obtained through spray drying. The DPP-IV inhibition rate is 69.44% + / -2.66%-87.45% + / -1.25%, and the compound can be used for assisting in reducing blood sugar of consumers and reducing the dosage of hypoglycemic peptides so as to reduce side effects. The hypoglycemic peptides in the enzymolysis sheep milk are separated, purified and identified through ultrafiltration, preparative chromatography and HPLC-MS / MS, 12 hypoglycemic peptides from sheep milk protein are obtained, and theoretical and technical supports are provided for development of hypoglycemic assisting dairy products.

The invention relates to a PKM2-based nano glycopeptide activator and application thereof.The nano glycopeptide activator comprises three functional units, namely a PKM2 targeted activation unit R3, a self-assembly unit R2 and a response unit R1, and the chemical structure of the nano glycopeptide activator is shown in the formula (I). After administration, the nano glycopeptide activator can be enriched to a tumor site through targeting of sugar receptor transporter protein or an EPR effect; after entering tumor cells, the polypeptide is subjected to in-situ fibrosis deformation under the hydrolysis action of glycohydrolase, so that specific targeting and retention are realized; the nano-fiber with the PKM2 targeting activation unit can promote conversion of dimer PKM2 into tetramer PKM2, inhibit nucleation of PKM2, and prevent the DNA damage repair process of tumor cells.

The invention belongs to the field of biosensing and biological interface engineering, and particularly relates to a double-enhanced bionic glycopeptide electrochemical biosensor based on anti-pollution and anti-enzymolysis stability and a preparation method of the double-enhanced bionic glycopeptide electrochemical biosensor. The invention is inspired by glycoprotein in vascular endothelial cell glycocalyx, a novel glycopeptide material capable of enhancing biological pollution resistance and protease hydrolysis resistance stability is constructed by performing glycosylation modification at the C terminal of a polypeptide sequence, and the novel glycopeptide material is applied to a complex biological medium and has remarkable performance superior to that of non-glycosylated peptide.