142 results about "Enzymatic degradation" patented technology

Methods, compositions, and systems for steeping, propagation and fermentation, particularly large-scale operations for production of starch and ethanol and fermentation product streams are provided. Addition of mycotoxin mitigating enzymes or microorganisms expressing mycotoxin mitigating enzymes to steeping, propagation, and / or fermentation tanks, and / or to post-fermentation product streams, mitigates mycotoxin levels in fermentation co-products obtained from mycotoxin contaminated feedstocks.

The present invention relates to novel carbamate hydrolases for the enzymatic decomposition of polyurethanes and to an enzymatic process for the complete decomposition of polyurethanes into defined monomers.

The present disclosure provides variant enzymes for use in the enzymatic degradation of polyethylene terephthalate (PET).

The present invention relates to new urethanases for the enzymatic breakdown of polyurethanes and to an enzymatic process for the complete breakdown of polyurethanes into defined monomers.

The invention belongs to the technical field of nuclear medicine molecular diagnosis and treatment, and discloses a modified CAIX targeting cyclic peptide and a nuclide marker and application thereof. According to the CAIX targeted cyclic peptide, a rigid bifunctional chelating agent RESCA is introduced to replace a traditional chelating agent, and a sulfonated or alkylated amino acid connector is combined, so that the enzymolysis resistance and in-vivo stability of the probe are remarkably improved. By optimizing the structure of the connector, the lipophilicity is reduced, liver and gall metabolism and non-specific uptake are reduced, and the high uptake rate of tumor target tissues is maintained. The cyclic peptide can label a diagnostic nuclide for PET imaging, or a therapeutic nuclide for intra-tumor irradiation therapy, using DOTA or NOTA in combination with an alkylated amino acid linker or an albumin ligand. Experiments show that the marker has high radiochemical purity, excellent pharmacokinetic characteristics and low kidney retention, and is suitable for precise diagnosis and targeted therapy of CAIX high expression tumors such as kidney cancer, colorectal cancer and brain glioma.

The application discloses a composite degradation preparation for degrading microplastics in wastewater and an application method thereof, and relates to the technical field of wastewater purification. The preparation is prepared by three steps of preparing a rare earth doped titanium-based porous framework, preparing an enzyme-containing bacteria coated precursor solution, and in-situ cross-linking and co-fixing. The framework realizes visible light response and magnetic separation characteristics. The co-fixing of enzyme and bacteria is completed by microcapsule embedding and carrier adsorption cross-linking. The preparation integrates the functions of photocatalysis, enzymatic degradation and microbial degradation. In application, the wastewater is pretreated to SS≤60mg / L, and then the preparation is added to a degradation tank. Under the conditions of 395-450nm light, 25-40 DEG C, and pH 6.0-8.0, the wastewater is degraded by stage light irradiation. Finally, the preparation is recycled by magnetic separation. The preparation solves the problem of free radical damage in the light-biological synergy, and has strong impact resistance, high microplastic removal rate, and recyclable preparation. The preparation is suitable for continuous treatment of wastewater, greatly reduces the operation cost, and is suitable for purification treatment of various wastewater containing microplastics.

The application belongs to the technical field of bioengineering, and particularly relates to a xanthan gum cleavage ancestral enzyme AncXLY196 based on ancestral sequence reconstruction, an amino acid sequence of which is shown as SEQ ID NO. 2. Based on the xanthan gum cleavage ancestral enzyme, a mutant AncXLY196-X7 is further obtained, an amino acid sequence of which is shown as SEQ ID NO. 4, xanthan gum degradation activity of which reaches 2.5 times of that of the xanthan gum cleavage ancestral enzyme, and the optimal catalytic temperature of the xanthan gum cleavage ancestral enzyme and the mutant thereof is increased to 45 DEG C, and the enzyme activity of the mutant of the xanthan gum cleavage ancestral enzyme is reserved by 50-80% after being treated at 45-55 DEG C for 12-24 h. The application provides a new enzyme resource for efficient enzymatic degradation of xanthan gum and preparation of functional oligosaccharides.

The present invention relates to a process for enzymatic depolymerization of polyethylene terephthalate (PET), in particular contained in a plastic material. The process according to the invention can in particular be implemented on an industrial or semi-industrial scale.

The present invention relates to a process for purifying phycocyanins from a solution comprising phycocyanins and glycogen, said phycocyanins being produced by microorganisms belonging to the order Cyanidiales, characterized in that it comprises (i) an enzymatic degradation step of glycogen with an enzyme suitable for degrading glycogen at a pH below 6 and a reaction temperature below 40°C, said enzyme being selected from a pectinase, a glucoamylase, or a mixture of a pectinase and a pullulanase, and (ii) a step of separating phycocyanins from the glycogen degradation products.

The present invention provides a novel means for degrading PEF. Provided is a method for degrading polyethylene furanoate that includes causing an enzyme comprising a polypeptide which has an amino acid sequence having at least 90% identity with the amino acid sequence of SEQ ID NO: 1 and has one or more amino acid substitutions selected from the group consisting of H129W, F208S, A172C, and A209C to act on polyethylene furanoate.

Disclosed herein is a polymeric microparticle comprising a polymeric material that is susceptible to enzymatic degradation and a dye encapsulated within the polymeric material Also disclosed herein are microfluidic devices and methods making use of said microparticles.

The invention discloses a lotus seed wine brewing method, and belongs to the technical field of wine brewing, selenium-rich lotus seeds without lotus cores are used as a main material, the lotus cores are collected, bitter root sources are eliminated through warm water debitterizing and enzymolysis degradation, the lotus cores and rice husks are compounded and incorporated into an auxiliary material system, the rice husks adsorb residual bitter taste, selenium and flavone of the lotus cores are synchronously reserved, and the lotus seed wine is obtained. Waste utilization of the lotus seeds is facilitated, and beneficial components of the lotus seed wine are improved; in the cleaning link, normal-temperature sterile water containing food-grade oligosaccharide is adopted for low-pressure spraying, the oligosaccharide forms a protective film on the surfaces of the lotus seeds, and selenium dissolution is reduced; a low-pressure low-temperature short-time process is adopted in the cooking link, so that organic selenium and flavone are prevented from being damaged by high temperature; low-temperature sectional interception is adopted for distillation, selenium is prevented from being volatilized along with high-temperature water vapor, and active ingredients are intensively intercepted in a core wine section. According to the invention, the value of the raw materials is maximized, active ingredients are efficiently reserved, the production efficiency and the quality stability are improved, and finally, the wine has drinking experience and health-care properties of lotus seeds.

This invention provides a collagen biphase crosslinked material, its preparation method, and an implantable scaffold. It relates to compositions of polymeric compounds and also to the field of medical materials technology. This application provides a method for preparing a collagen biphase crosslinked material. During the nucleation phase, a low concentration of hydrogen peroxide is introduced to synergistically promote collagen self-assembly and hydrogen peroxide crosslinking. During the growth phase, a higher concentration of hydrogen peroxide solution is added to allow the covalent crosslinked network to form synchronously with fiber extension. The growth phase self-assembly control process can significantly extend the collagen self-assembly time, resulting in more thorough crosslinking and achieving structure-function synergy at the molecular scale. Finally, hydrogen peroxide decomposes into water and oxygen, leaving no crosslinking agent residue. The prepared collagen biphase crosslinked material exhibits good mechanical properties and resistance to enzymatic degradation, and the complete in vivo degradation time can be controlled between 12 and 24 months.

This invention provides a high-recovery heme iron extraction method and apparatus using enzymatic hydrolysis-multi-stage centrifugal coupling, relating to the fields of biopharmaceuticals and fluid dynamics processing technology. The method first constructs a polyanionic electrolyte shielding system, utilizing electrostatic potential energy to induce conformational instability of globin and pre-exposure of hydrophobic cavities, reducing binding energy at the molecular level and solving the problem of heme's encapsulation resistance in high-concentration systems. Then, high-shear coupled enzymatic hydrolysis is performed within a micron-sized liquid film generated by a hypergravity field, utilizing the instantaneous synergy of mechanical shear stress and enzymatic degradation to achieve directional exfoliation of heme, eliminating secondary re-adhesion effects. Finally, directional phase transition regulation is performed based on density gradient drive, and a high-frequency pulsed pressure field is introduced for in-situ exchange washing, distinguishing and retaining impurities and crystal cores, outputting a high-purity, flavor-neutral heme iron product. This invention effectively improves the controllability of the extraction process and the stability of industrial continuous production.

The invention relates to a fabric anti-wrinkle finishing agent and a preparation method thereof. The fabric anti-wrinkle finishing agent is prepared from the following raw materials in percentage by mass: 30-40% of modified chitosan, 25-32% of citric acid, 2-4% of tea tree essential oil, 3-5% of dextrin, 1-3% of sorbic acid, 5-10% of glycerol and 13-27% of deionized water. Wherein the modified chitosan is a chitosan degradation product obtained by carrying out enzymatic degradation reaction and drying treatment on chitosan and a papaya extract. The invention provides a fabric anti-wrinkle finishing agent containing modified chitosan, and the modified chitosan is a chitosan degradation product obtained by carrying out enzymatic degradation reaction and drying treatment on chitosan and a papaya extract, experiments prove that when the fabric anti-wrinkle finishing agent is used for treating the fabric, the anti-wrinkle performance and the color fastness of the fabric can be remarkably improved, and particularly the color fastness to perspiration and acid stain can be remarkably improved.

The invention discloses hydrogel loaded with repair-promoting and antioxidant peptides as well as a preparation method and application of the hydrogel, and belongs to the technical field of preparation of medical materials. According to the preparation method, firstly, polypeptide with the amino acid sequence of CEYWVKWCGEKGPAGERG is synthesized, and the polypeptide has antioxidant activity and cell proliferation promoting activity and has good structural stability and enzymatic degradation resistance; and loading the polypeptide into the hyaluronic acid hydrogel to obtain the hyaluronic acid hydrogel. The prepared hydrogel loaded with the repair-promoting and antioxidant peptide has excellent injectable performance and cornea spreadability, can enhance the retention of the polypeptide on the ocular surface, effectively solves the technical problem of high administration frequency of the existing preparation, has good cell and tissue compatibility, and has no adverse reaction in vivo.

The invention belongs to the field of gene engineering, and particularly relates to a PU hydrolase UMG-SP1 mutant with high catalytic activity and application thereof. The amino acids at the 126, 173, 202, 364, 302, 172, 178, 89, 129, 298, 79 and 201 sites in the amino acid sequence of the PU depolymerases as shown in SEQ ID NO: 1 are mutated by using structural analysis and site-specific mutagenesis technologies to obtain a plurality of mutants, and the mutants with improved catalytic activity are obtained. Experiments prove that the PU depolymerization mutants improve the degradation capacity of PU plastic by promoting the hydrolysis efficiency of carbamate bonds in PU, and an important degrading enzyme element is provided for developing an efficient and environment-friendly PU plastic enzymatic degradation technology.

Described herein are novel oligosaccharides of cannabidiol and other cannabinoids and methods of production and uses thereof. The production method includes contacting cannabidiol with UDP-carbohydrate dependent glycosyltransferases (UGT73A class) to produce a cannabinoid-β-D-glycoside, then contacting the product with a cyclomaltodextrin glucanotransferase and a cyclodextrin to elongate the sugar moiety with (1-4)-α-D-glucopyranoside, thereby producing a mixed 0-a-structure in a recombinant host that has an amphiphilic structure as a surfactant solubilizer and different enzymatic degradability of α- and β-D-glucopyranose residues by amylases and β-glycosidases, thereby allowing targeted drug delivery.

The invention provides a controllable photo-thermal-enzymatic degradation porous material as well as a preparation method and application thereof. The controllable photo-thermal-enzymatic degradation porous material is prepared from the following raw materials: an oil phase mixture and a water phase mixture, the oil phase mixture comprises an enzyme sensitive polymer base material, a random heteropolymer (RHP)-enzyme compound, Janus photo-thermal nanoparticles, an internal phase dispersing agent and a solvent; the aqueous phase mixture comprises water, electrolyte and alginate. According to the porous material provided by the invention, the Janus photo-thermal nanoparticles and the RHP-enzyme compound are coupled, under near-infrared light, RHP conformational change can be triggered to release BC lipase, degradation of the porous material can be triggered under the mild condition of 37 DEG C, and the degradation rate within 48 hours can reach 98%.

The invention discloses a novel application of ginkgo biflavone in lung cancer treatment, belongs to the technical field of medicines, and researches show that ginkgo biflavone can target CDC7 and is a novel targeted medicine. It is confirmed for the first time that ginkgo biflavone regulates and controls the thermal stability and enzymatic degradation of ginkgo biflavone through targeting CDC7 kinase (binding free energies-8.3 kcal / mol), directly intervenes in the DNA replication process, and provides a natural drug lead compound for development of a novel CDC7 small-molecule inhibitor. The compound has great significance in preventing and treating malignant tumors, and has a wide prospect in the aspect of preparing medicines for treating lung tumors.

The invention relates to novel urethanases for the enzymatic degradation of polyurethanes and to an enzymatic process for the complete degradation of polyurethanes in defined monomers.

An enzyme includes an amino acid sequence with, at least, a 80% sequence identity to sequence SEQ ID NO: 1, for fumonisin degradation in a sample. A method for fumonisin degradation is based on the incubation of the contaminated sample with an enzyme comprising an amino acid sequence with, at least, a 80% sequence identity to sequence SEQ ID NO: 1.

The application belongs to the technical field of biological medicine, and specifically discloses preparation and application of self-assembled glycopeptide nanoparticles for mRNA vaccine delivery, wherein the nanoparticles are formed by self-assembly of a glycopeptide carrier, mRNA and manganese ions. In the nanoparticle preparation process, the manganese ions are adsorbed onto tryptophan and histidine through metal chelation, and play an adjuvant role in activating the cGAS-STING pathway after administration. The nanoparticles can effectively adsorb mRNA, can stably exist for 4 weeks at 4 DEG C, protect mRNA from enzymatic degradation, promote intracellular mRNA internalization and lysosome escape, effectively transfect eGFP-mRNA and Fluc-mRNA in DC2.4 and BMDC cells, and effectively target mRNA delivery to lymph nodes, activate the cGAS-STING pathway, effectively activate the specific immune system in a B16F10-OVA prevention model, delay tumor growth, and prolong the survival cycle of mice.

The invention relates to the field of soil pollution remediation, and discloses a micro-ecological reconstruction and remediation method for micro-plastic polluted soil, which comprises the following steps: firstly, screening and enriching micro-plastic degradation functional florae which have the advantages of pseudomonas and bacillus, and the bacillus can secrete lipase and protease; the preparation method comprises the following steps: mixing biomass charcoal prepared by pyrolysis of corn straws with a functional bacterium suspension for adsorption, and carrying out sodium alginate-calcium chloride crosslinking to prepare an immobilized composite repairing agent; applying a repairing agent accounting for 1-5% of the mass of the soil, uniformly mixing the repairing agent with the soil, regulating the soil humidity to 20-30% and the carbon-nitrogen ratio to (15-25): 1, and maintaining the environment temperature of 15-35 DEG C; microplastics are removed through the synergistic effect of biomass charcoal adsorption and functional flora enzymatic degradation, beneficial microorganisms are enriched synchronously, and functional gene expression is activated; the problems that in the prior art, functional flora planting is difficult, the degradation efficiency is low, micro-ecological restoration is deficient, and the environmental adaptability is poor are solved.

The present invention discloses a microbial composting agent for straw and its preparation method, belonging to the field of microbial technology. The microbial composting agent for straw of the present invention is prepared from the following raw materials in parts by weight: 15-18 parts of functional microbial liquid, 3-5 parts of neutral protease, 1-3 parts of laccase, 20-25 parts of nano-porous diatomite carrier, 5-7 parts of humectant, 2-3 parts of humic acid chelated trace elements, and 2-4 parts of inducer. The present invention screens Thermophilic Talaromyces, Thermoactinomyces guangxiensis, and Arthrobacter terregens to form a functional microbial liquid, adds a laccase-protease complex system in combination with the physical adsorption effect of diatomite, adds humic acid chelated trace elements to form an ion exchange network with polyglutamic acid, and efficiently degrades straw components, accelerates the composting process, and improves the quality of straw compost through the triple mechanisms of "enzymatic degradation - element supplementation - microenvironment regulation".

The present invention provides a GHRH agonist modified with long-chain fatty acylation at the C-terminus. Compared with natural GHRH and existing GHRH agonists, the GHRH agonist modified with long-chain fatty acylation at the C-terminus exhibits significantly improved biological activity and is less susceptible to enzymatic degradation in vivo, thereby showing better stability. The serum half-life exceeds 24 hours, enabling long-acting agonism of GHRH receptors and exerting various therapeutic effects.

The present invention provides a novel means for efficiently degrading PEF. Provided is a method for degrading polyethylene furanoate and polyethylene terephthalate that includes causing an enzyme comprising a polypeptide that has an amino acid sequence having at least 90% identity with the amino acid sequence of SEQ ID NO: 1 to act on polyethylene furanoate and polyethylene terephthalate in a buffer solution having a pH of 8.5 to 11.

The invention discloses a POSTN targeting peptide and a mutant thereof. The POSTN targeting peptide comprises a sequence as shown in SEQ ID NO: 1 and the mutant thereof. The POSTN targeting peptide disclosed by the invention not only has a relatively good blocking effect on POSTN protein, but also has relatively good enzymatic degradation resistance and an effect of inhibiting tumor growth. Moreover, compared with an antibody drug, the POSTN targeting peptide not only has excellent biological activity and biological safety, but also has the advantages of simple structure, easiness in modification and transformation due to carrying of a plurality of functional groups, low synthesis cost and the like.

The invention relates to the technical field of biological catalysis engineering, and discloses a method for preparing enteromorpha oligosaccharide through composite enzymatic hydrolysis gradient alcohol precipitation, which comprises the following steps: in the enzymatic hydrolysis reaction process, establishing negative feedback coupling between stirring power and ethanol concentration increase operation, characterizing the consumption degree of a non-liquid-phase substrate in real time by using the stirring power, and calculating the concentration of the enteromorpha oligosaccharide. According to the present invention, the strong coupling relationship between the chemical reaction and the physical phase change is established, such that the enzymolysis is converted from the random collision process to the physical phase equilibrium-dominated deterministic ordered evolution process so as to achieve the selective precipitation and degradation of the product; and the capability of the system for automatically adapting to the raw material difference is improved, and the technical problems of disordered reaction and poor product selectivity in the existing enzymatic degradation are solved.