17 results about "Enzymatic degradation" patented technology

Methods, compositions, and systems for steeping, propagation and fermentation, particularly large-scale operations for production of starch and ethanol and fermentation product streams are provided. Addition of mycotoxin mitigating enzymes or microorganisms expressing mycotoxin mitigating enzymes to steeping, propagation, and / or fermentation tanks, and / or to post-fermentation product streams, mitigates mycotoxin levels in fermentation co-products obtained from mycotoxin contaminated feedstocks.

The application discloses a composite degradation preparation for degrading microplastics in wastewater and an application method thereof, and relates to the technical field of wastewater purification. The preparation is prepared by three steps of preparing a rare earth doped titanium-based porous framework, preparing an enzyme-containing bacteria coated precursor solution, and in-situ cross-linking and co-fixing. The framework realizes visible light response and magnetic separation characteristics. The co-fixing of enzyme and bacteria is completed by microcapsule embedding and carrier adsorption cross-linking. The preparation integrates the functions of photocatalysis, enzymatic degradation and microbial degradation. In application, the wastewater is pretreated to SS≤60mg / L, and then the preparation is added to a degradation tank. Under the conditions of 395-450nm light, 25-40 DEG C, and pH 6.0-8.0, the wastewater is degraded by stage light irradiation. Finally, the preparation is recycled by magnetic separation. The preparation solves the problem of free radical damage in the light-biological synergy, and has strong impact resistance, high microplastic removal rate, and recyclable preparation. The preparation is suitable for continuous treatment of wastewater, greatly reduces the operation cost, and is suitable for purification treatment of various wastewater containing microplastics.

The application belongs to the technical field of bioengineering, and particularly relates to a xanthan gum cleavage ancestral enzyme AncXLY196 based on ancestral sequence reconstruction, an amino acid sequence of which is shown as SEQ ID NO. 2. Based on the xanthan gum cleavage ancestral enzyme, a mutant AncXLY196-X7 is further obtained, an amino acid sequence of which is shown as SEQ ID NO. 4, xanthan gum degradation activity of which reaches 2.5 times of that of the xanthan gum cleavage ancestral enzyme, and the optimal catalytic temperature of the xanthan gum cleavage ancestral enzyme and the mutant thereof is increased to 45 DEG C, and the enzyme activity of the mutant of the xanthan gum cleavage ancestral enzyme is reserved by 50-80% after being treated at 45-55 DEG C for 12-24 h. The application provides a new enzyme resource for efficient enzymatic degradation of xanthan gum and preparation of functional oligosaccharides.

The present invention relates to a process for purifying phycocyanins from a solution comprising phycocyanins and glycogen, said phycocyanins being produced by microorganisms belonging to the order Cyanidiales, characterized in that it comprises (i) an enzymatic degradation step of glycogen with an enzyme suitable for degrading glycogen at a pH below 6 and a reaction temperature below 40°C, said enzyme being selected from a pectinase, a glucoamylase, or a mixture of a pectinase and a pullulanase, and (ii) a step of separating phycocyanins from the glycogen degradation products.

This invention provides a high-recovery heme iron extraction method and apparatus using enzymatic hydrolysis-multi-stage centrifugal coupling, relating to the fields of biopharmaceuticals and fluid dynamics processing technology. The method first constructs a polyanionic electrolyte shielding system, utilizing electrostatic potential energy to induce conformational instability of globin and pre-exposure of hydrophobic cavities, reducing binding energy at the molecular level and solving the problem of heme's encapsulation resistance in high-concentration systems. Then, high-shear coupled enzymatic hydrolysis is performed within a micron-sized liquid film generated by a hypergravity field, utilizing the instantaneous synergy of mechanical shear stress and enzymatic degradation to achieve directional exfoliation of heme, eliminating secondary re-adhesion effects. Finally, directional phase transition regulation is performed based on density gradient drive, and a high-frequency pulsed pressure field is introduced for in-situ exchange washing, distinguishing and retaining impurities and crystal cores, outputting a high-purity, flavor-neutral heme iron product. This invention effectively improves the controllability of the extraction process and the stability of industrial continuous production.

The present invention provides a GHRH agonist modified with long-chain fatty acylation at the C-terminus. Compared with natural GHRH and existing GHRH agonists, the GHRH agonist modified with long-chain fatty acylation at the C-terminus exhibits significantly improved biological activity and is less susceptible to enzymatic degradation in vivo, thereby showing better stability. The serum half-life exceeds 24 hours, enabling long-acting agonism of GHRH receptors and exerting various therapeutic effects.

This invention discloses a BhrPETase keratinase mutant and its application in the degradation of polyethylene terephthalate (PET), belonging to the fields of environmental science and enzyme engineering. The site numbers are based on the amino acid sequence shown in SEQ ID NO:1. The mutant contains substitutions of S14G, H78Y, and S184G relative to SEQ ID NO:1, with the preferred amino acid sequence shown in SEQ ID NO:9. Under conditions of 1 mg enzyme protein / g substrate, 65°C, and 96 h, the preferred mutant exhibits a degradation rate of 90.4% for PET membranes and a half-life of 150 h at 60°C, making it suitable for enzymatic degradation and depolymerization of PET.

A C-terminal truncated alginate lyase and its application. This invention belongs to the fields of bioengineering, genetic engineering, and enzyme engineering, and relates to a C-terminal truncated alginate lyase TY2 designed with molecular docking assistance, its encoding gene, recombinant vector, strain, and its application in the preparation of low-polymerization degree alginate oligosaccharides. Using wild-type MhAly6 as the parent, the truncation boundary was determined through structural prediction and molecular docking. A portion of the linker peptide was retained, and the C-terminal GH28 family domain was deleted to obtain the truncated form TY2. TY2 has approximately twice the specific activity of the wild-type enzyme, and even higher residual activity after incubation at 45°C for 1 hour, achieving a synergistic improvement in catalytic activity and thermal stability. + The relative enzyme activity reached 4.3 times that of the control group, which can degrade pretreated Sargassum powder. The product is mainly ΔDP2–ΔDP4 low-polymerization brown algae oligosaccharides, which are suitable for related enzymatic degradation and oligosaccharide preparation.

This invention belongs to the field of microbial technology, and discloses a highly efficient anaerobic fungal strain for degrading lignocellulose in the rumen of Dulong cattle, its cultivation method, and its applications. The strain is *Neisseria gonorrhoeae*. Joblinomyces apicalis DLM2, deposited at the China Center for Type Culture Collection (CCTCC) under accession number CCTCC NO: M 20252927, was isolated from the rumen of Dulong cattle and can be stably cultured under strictly anaerobic conditions. This strain possesses a single-centered cell line and filamentous rhizoids, enabling it to efficiently degrade lignocellulose through a synergistic effect of physical colonization and enzymatic degradation. It exhibits broad adaptability to natural straws such as rice straw and bamboo branches, as well as purified substrates such as microcrystalline cellulose and cellobiose. Its enzyme production exhibits substrate-inducible properties, and fermentation produces various metabolites including formic acid, acetic acid, and ethanol, demonstrating high lignocellulose degradation efficiency. This invention relates to an inoculum containing this strain and its application in lignocellulose degradation, roughage fermentation, agricultural straw resource utilization, and biomass conversion, providing new strain resources and technical support for the efficient degradation and resource utilization of lignocellulose.

This invention discloses a logic-responsive biomolecule carrier based on a phase separation system, its construction method, and its applications. The carrier is based on the attraction between cationic peptides with different logic responses and negatively charged biomolecules such as DNA or RNA, driving liquid-liquid phase separation and forming droplet-like particle structures. The carrier of this invention can selectively use protein kinase A (PKA) to phosphorylate peptides and use matrix metalloproteinases (MMPs) or glutathione (GSH) to cleave the peptide chain, thereby changing the charge density of the peptide to control the controlled release of biomolecules. It exhibits excellent biocompatibility, resistance to enzymatic degradation, and transfection efficiency, enabling effective delivery of biomolecules.

The application discloses a kind of fresh-keeping processing technology for reducing angelica polysaccharide loss, and relates to the technical field of traditional Chinese medicinal material origin processing, comprising the following steps: S1, material selection, S2, cleaning, S3, enzyme treatment, S4, cutting, S5, subsection drying.The application combines the technical means of time-limited water washing after harvesting, time-limited enzyme treatment after cleaning, fresh-cutting and gradient temperature subsection drying organically, and produces synergistic effect, wherein the enzyme treatment can effectively inhibit the activity of polysaccharide degradation enzyme in angelica fresh root, and block the enzymatic degradation pathway of polysaccharide from the source, and the gradient temperature subsection drying can significantly reduce the thermal degradation damage of polysaccharide at high temperature while ensuring the dehydration efficiency, and the comparative experiment of the embodiment shows that the polysaccharide retention rate of angelica decoction pieces processed by the application is significantly higher than that of traditional fresh-keeping processing technology and traditional dry-cutting technology, and the polysaccharide loss is effectively reduced.

The application provides a preparation method of a trehalose nanogel carrier system for delivering recombinant human elastin, and belongs to the technical field of biological medicine and cosmetics. The carrier system is prepared by using trehalose acrylate and acrylamide as monomers, preparing a nanogel core through a reverse microemulsion polymerization method, and sequentially constructing a hyaluronic acid surface modification and a glutathione-responsive recombinant human elastin compound loading. The system has a triple controlled release mechanism: amide catalytic hydrolysis realizes pH response slow release, hyaluronidase enzymatic degradation enhances epidermal permeation, and glutathione triggers disulfide bond rupture to realize intracellular targeted release. The nanogel has an encapsulation efficiency higher than 85%, a 72-hour cumulative release rate of 63% in PBS, and can significantly improve the transdermal absorption rate of recombinant human elastin and the intracellular bioavailability. The carrier system has good stability and high biocompatibility, and can be used for preparing high-efficiency anti-aging skin care products.

The present invention relates to an injectable microphase-separated hydrogel, a preparation method therefor, and use thereof. The hydrogel prepared by the process of the present invention uses collagen as a raw material. By controlling the steps of dissolution, room-temperature stirring, low-temperature stirring, and chemical crosslinking, a uniform bubble solution is preferentially formed in the process of room-temperature stirring; then, microphase separation occurs by triggering the temperature-sensitive characteristic of the collagen material at a low temperature; with the increase of the viscosity of the solution, the bubble morphology tends to be stable, and continuous stirring enables the collagen solution to reach a thermodynamic and kinetic steady state, forming a stable and uniform bubble solution; finally, by means of chemical crosslinking, a chemically crosslinked network is formed, in which three-dimensional networks and microsphere structures are interlaced. These two structures together endow the microphase-separated hydrogel with strong support, resistance to heat and humidity, resistance to enzymatic degradation, ability to be injected, and injection uniformity. Using this process, the hydrogel still maintains good performance after high-temperature and high-pressure treatment, and has a white appearance, thereby avoiding the Tyndall phenomenon after injection.

This invention belongs to the field of biomedical technology, specifically disclosing a CD300LF affinity peptide and its applications. The CD300LF affinity peptide provided by this invention can specifically bind to the CD300LF protein and can be used to prepare peptide drugs or detection reagents targeting CD300LF, expanding the forms of drug / formulation development targeting CD300LF. Experiments have shown that the CD300LF affinity peptide can effectively reprogram tumor-associated macrophages, reverse the polarization of TAMs from M2 to M1, and promote the production of M1-related cytokines, thereby enhancing the phagocytic capacity of macrophages against tumor cells. The CD300LF affinity peptide provided by this invention exhibits significant tumor-suppressing effects and immune-activating effects in in vivo tumor models. Its modified peptide has excellent in vivo stability and anti-enzymatic degradation ability, and has no obvious toxic side effects, showing good prospects for translation and industrialization.

Orally-ingestible supplement formulations for boosting NAD+ (Nicotinamide adenine dinucleotide) in a human and methods of use. The formulations provide a Triple Boost for NAD+ that will combine 3 or more different ingredients each of which uses a different metabolic pathway selected from the following: a) the De Novo Pathway, b) the Preiss-Handler Pathway, c) the Salvage Pathway, d) the Reduced NAD+ Precursor Pathway, and e) CD38 Inhibition Pathway.

This invention discloses a fully absorbable PDO-P4HB binary composite gradient degradable hernia repair patch, comprising: a PDO support layer, a P4HB toughening layer, and multiple composite connection nodes and edge reinforcement rings composed of PDO-P4HB blend materials. This invention uses a fully absorbable and non-immunogenic PDO+P4HB composite material, which can be completely degraded into non-toxic products and excreted from the body through hydrolysis and enzymatic degradation postoperatively, reducing the risk of complications such as infection and adhesions. It is suitable for complex repair scenarios such as infected abdominal wall defects. Utilizing the inherent difference in degradation rates between the two materials, and through specific weaving structures and parameter design, a gradient degradation structure is constructed to achieve a dynamic adaptation process of "early high-strength support (PDO-dominated) – mid-term flexible transition (synergistic between the two) – late complete degradation (P4HB finishing)," meeting both the rigidity requirements of intraoperative operations and the mechanical and biological needs of each stage of tissue repair, balancing support stability and tissue ingrowth.

The application discloses a multi-channel enzyme typing foot fungus reversible detection patch, and belongs to the technical field of biological detection and shoe functional material. The patch comprises a breathable and dye-resistant surface layer, a multi-channel enzyme sensing middle layer and a low-sensitivity back adhesive bottom layer. The sensing middle layer is a heterogeneous four-layer composite structure formed by a phase separation process, comprising a polyurethane continuous phase layer and a superabsorbent resin dispersed phase layer, which respectively load a ternary acid-base indicator and a fungus cell wall specific degradation enzyme. The application releases acidic substances by enzymatic degradation of the fungus cell wall, realizes multi-color typing by using the difference in metabolic acidity of different fungi, and restores the color after removing the detection environment, thereby realizing reusability. The application effectively eliminates the interference of sweat, bacteria and the like, solves the false positive / false negative problem of the existing humidity color-changing insole, has the advantages of high specificity, high accuracy and non-onetime use, and can be widely applied to the shoe material integration field.