164 results about "Enzymatic degradation" patented technology

Methods, compositions, and systems for steeping, propagation and fermentation, particularly large-scale operations for production of starch and ethanol and fermentation product streams are provided. Addition of mycotoxin mitigating enzymes or microorganisms expressing mycotoxin mitigating enzymes to steeping, propagation, and / or fermentation tanks, and / or to post-fermentation product streams, mitigates mycotoxin levels in fermentation co-products obtained from mycotoxin contaminated feedstocks.

The present invention relates to novel carbamate hydrolases for the enzymatic decomposition of polyurethanes and to an enzymatic process for the complete decomposition of polyurethanes into defined monomers.

The present disclosure provides variant enzymes for use in the enzymatic degradation of polyethylene terephthalate (PET).

The invention discloses a microbial decomposing inoculant for straws and a preparation method thereof, and belongs to the technical field of microorganisms. The microbial decomposing inoculant for straw is prepared from the following raw materials in parts by weight: 15-18 parts of functional microbial liquid, 3-5 parts of neutral protease, 1-3 parts of laccase, 20-25 parts of a nano-porous diatomite carrier, 5-7 parts of a humectant, 2-3 parts of humic acid chelated trace elements and 2-4 parts of an inducer. The preparation method comprises the following steps: screening talaromyces thermophilus, Guangxi thermoactinomycetes and arthrobacter habitualis to form a functional microbial liquid, adding a laccase-protease compounding system to cooperate with the physical adsorption effect of diatomite, and adding humic acid chelated trace elements to form an ion exchange network with polyglutamic acid; straw components are efficiently degraded through a triple mechanism of'enzymatic degradation-element supplementation-microenvironment regulation and control ', the decomposition process is accelerated, and the straw compost quality is improved.

The present invention relates to new urethanases for the enzymatic breakdown of polyurethanes and to an enzymatic process for the complete breakdown of polyurethanes into defined monomers.

The invention discloses an efficient continuous PET (polyethylene terephthalate) degradation process based on a membrane method technology, which comprises the following steps: mixing an immobilized enzyme compound, a PET raw material to be degraded and a glycine buffer solution to form a reaction solution for degradation, then constructing a'continuous reactor 'by using two size screening membranes, performing primary separation by using an ultrafiltration membrane or a microfiltration membrane, and performing secondary separation by using an ultrafiltration membrane or a microfiltration membrane. A nanofiltration membrane or a reverse osmosis membrane is adopted for secondary separation of the degradation product, automatic separation of the degradation product and a reaction system and automatic purification of the degradation product are achieved, and then the enzymatic degradation efficiency and the product recovery efficiency are improved. Through the construction of the continuous reactor, the automatic PET degradation is realized, so that the costs in the aspects of labor, time, economy and the like are remarkably saved. The undegraded PET and a small amount of exfoliative enzyme can be circulated back to the reaction kettle after being intercepted by the size screening membrane, and the process not only can promote full contact between the undegraded PET and the hydrolase, but also can reduce the loss of the enzyme in the degradation process.

The invention belongs to the technical field of nuclear medicine molecular diagnosis and treatment, and discloses a modified CAIX targeting cyclic peptide and a nuclide marker and application thereof. According to the CAIX targeted cyclic peptide, a rigid bifunctional chelating agent RESCA is introduced to replace a traditional chelating agent, and a sulfonated or alkylated amino acid connector is combined, so that the enzymolysis resistance and in-vivo stability of the probe are remarkably improved. By optimizing the structure of the connector, the lipophilicity is reduced, liver and gall metabolism and non-specific uptake are reduced, and the high uptake rate of tumor target tissues is maintained. The cyclic peptide can label a diagnostic nuclide for PET imaging, or a therapeutic nuclide for intra-tumor irradiation therapy, using DOTA or NOTA in combination with an alkylated amino acid linker or an albumin ligand. Experiments show that the marker has high radiochemical purity, excellent pharmacokinetic characteristics and low kidney retention, and is suitable for precise diagnosis and targeted therapy of CAIX high expression tumors such as kidney cancer, colorectal cancer and brain glioma.

The application discloses a composite degradation preparation for degrading microplastics in wastewater and an application method thereof, and relates to the technical field of wastewater purification. The preparation is prepared by three steps of preparing a rare earth doped titanium-based porous framework, preparing an enzyme-containing bacteria coated precursor solution, and in-situ cross-linking and co-fixing. The framework realizes visible light response and magnetic separation characteristics. The co-fixing of enzyme and bacteria is completed by microcapsule embedding and carrier adsorption cross-linking. The preparation integrates the functions of photocatalysis, enzymatic degradation and microbial degradation. In application, the wastewater is pretreated to SS≤60mg / L, and then the preparation is added to a degradation tank. Under the conditions of 395-450nm light, 25-40 DEG C, and pH 6.0-8.0, the wastewater is degraded by stage light irradiation. Finally, the preparation is recycled by magnetic separation. The preparation solves the problem of free radical damage in the light-biological synergy, and has strong impact resistance, high microplastic removal rate, and recyclable preparation. The preparation is suitable for continuous treatment of wastewater, greatly reduces the operation cost, and is suitable for purification treatment of various wastewater containing microplastics.

The application belongs to the technical field of bioengineering, and particularly relates to a xanthan gum cleavage ancestral enzyme AncXLY196 based on ancestral sequence reconstruction, an amino acid sequence of which is shown as SEQ ID NO. 2. Based on the xanthan gum cleavage ancestral enzyme, a mutant AncXLY196-X7 is further obtained, an amino acid sequence of which is shown as SEQ ID NO. 4, xanthan gum degradation activity of which reaches 2.5 times of that of the xanthan gum cleavage ancestral enzyme, and the optimal catalytic temperature of the xanthan gum cleavage ancestral enzyme and the mutant thereof is increased to 45 DEG C, and the enzyme activity of the mutant of the xanthan gum cleavage ancestral enzyme is reserved by 50-80% after being treated at 45-55 DEG C for 12-24 h. The application provides a new enzyme resource for efficient enzymatic degradation of xanthan gum and preparation of functional oligosaccharides.

The present invention relates to a process for enzymatic depolymerization of polyethylene terephthalate (PET), in particular contained in a plastic material. The process according to the invention can in particular be implemented on an industrial or semi-industrial scale.

The present invention relates to a process for purifying phycocyanins from a solution comprising phycocyanins and glycogen, said phycocyanins being produced by microorganisms belonging to the order Cyanidiales, characterized in that it comprises (i) an enzymatic degradation step of glycogen with an enzyme suitable for degrading glycogen at a pH below 6 and a reaction temperature below 40°C, said enzyme being selected from a pectinase, a glucoamylase, or a mixture of a pectinase and a pullulanase, and (ii) a step of separating phycocyanins from the glycogen degradation products.

The present invention provides a novel means for degrading PEF. Provided is a method for degrading polyethylene furanoate that includes causing an enzyme comprising a polypeptide which has an amino acid sequence having at least 90% identity with the amino acid sequence of SEQ ID NO: 1 and has one or more amino acid substitutions selected from the group consisting of H129W, F208S, A172C, and A209C to act on polyethylene furanoate.

Disclosed herein is a polymeric microparticle comprising a polymeric material that is susceptible to enzymatic degradation and a dye encapsulated within the polymeric material Also disclosed herein are microfluidic devices and methods making use of said microparticles.

The invention discloses a lotus seed wine brewing method, and belongs to the technical field of wine brewing, selenium-rich lotus seeds without lotus cores are used as a main material, the lotus cores are collected, bitter root sources are eliminated through warm water debitterizing and enzymolysis degradation, the lotus cores and rice husks are compounded and incorporated into an auxiliary material system, the rice husks adsorb residual bitter taste, selenium and flavone of the lotus cores are synchronously reserved, and the lotus seed wine is obtained. Waste utilization of the lotus seeds is facilitated, and beneficial components of the lotus seed wine are improved; in the cleaning link, normal-temperature sterile water containing food-grade oligosaccharide is adopted for low-pressure spraying, the oligosaccharide forms a protective film on the surfaces of the lotus seeds, and selenium dissolution is reduced; a low-pressure low-temperature short-time process is adopted in the cooking link, so that organic selenium and flavone are prevented from being damaged by high temperature; low-temperature sectional interception is adopted for distillation, selenium is prevented from being volatilized along with high-temperature water vapor, and active ingredients are intensively intercepted in a core wine section. According to the invention, the value of the raw materials is maximized, active ingredients are efficiently reserved, the production efficiency and the quality stability are improved, and finally, the wine has drinking experience and health-care properties of lotus seeds.

This invention provides a collagen biphase crosslinked material, its preparation method, and an implantable scaffold. It relates to compositions of polymeric compounds and also to the field of medical materials technology. This application provides a method for preparing a collagen biphase crosslinked material. During the nucleation phase, a low concentration of hydrogen peroxide is introduced to synergistically promote collagen self-assembly and hydrogen peroxide crosslinking. During the growth phase, a higher concentration of hydrogen peroxide solution is added to allow the covalent crosslinked network to form synchronously with fiber extension. The growth phase self-assembly control process can significantly extend the collagen self-assembly time, resulting in more thorough crosslinking and achieving structure-function synergy at the molecular scale. Finally, hydrogen peroxide decomposes into water and oxygen, leaving no crosslinking agent residue. The prepared collagen biphase crosslinked material exhibits good mechanical properties and resistance to enzymatic degradation, and the complete in vivo degradation time can be controlled between 12 and 24 months.

The invention provides a sulfonate type fluorinated surfactant with excellent degradability and emulsifying performance and a preparation method thereof, and relates to the field of fine chemical synthesis. According to the sulfonate type fluorine surfactant, firstly, halogenated fluoroalkane and fluoroolefin are subjected to a free radical polymerization reaction under the action of an initiator, methylene is introduced into a hydrophobic tail, biodegradation or enzymatic degradation can be conducted, and harm to a human body and ecological environment pollution are reduced; then replacing halogen atoms with hydroxyl under the heating condition of a sodium hydroxide aqueous solution, then carrying out ring-opening polymerization reaction with alkylene oxide to introduce alkoxy to increase the hydrophilicity of the surfactant, finally substituting hydrogen atoms in alcoholic hydroxyl with sodium, and then reacting with halogenated sodium sulfonate to introduce a sodium sulfonate group as a head group; the surface tension and the critical micelle concentration are lower.

This invention provides a high-recovery heme iron extraction method and apparatus using enzymatic hydrolysis-multi-stage centrifugal coupling, relating to the fields of biopharmaceuticals and fluid dynamics processing technology. The method first constructs a polyanionic electrolyte shielding system, utilizing electrostatic potential energy to induce conformational instability of globin and pre-exposure of hydrophobic cavities, reducing binding energy at the molecular level and solving the problem of heme's encapsulation resistance in high-concentration systems. Then, high-shear coupled enzymatic hydrolysis is performed within a micron-sized liquid film generated by a hypergravity field, utilizing the instantaneous synergy of mechanical shear stress and enzymatic degradation to achieve directional exfoliation of heme, eliminating secondary re-adhesion effects. Finally, directional phase transition regulation is performed based on density gradient drive, and a high-frequency pulsed pressure field is introduced for in-situ exchange washing, distinguishing and retaining impurities and crystal cores, outputting a high-purity, flavor-neutral heme iron product. This invention effectively improves the controllability of the extraction process and the stability of industrial continuous production.

The invention relates to a fabric anti-wrinkle finishing agent and a preparation method thereof. The fabric anti-wrinkle finishing agent is prepared from the following raw materials in percentage by mass: 30-40% of modified chitosan, 25-32% of citric acid, 2-4% of tea tree essential oil, 3-5% of dextrin, 1-3% of sorbic acid, 5-10% of glycerol and 13-27% of deionized water. Wherein the modified chitosan is a chitosan degradation product obtained by carrying out enzymatic degradation reaction and drying treatment on chitosan and a papaya extract. The invention provides a fabric anti-wrinkle finishing agent containing modified chitosan, and the modified chitosan is a chitosan degradation product obtained by carrying out enzymatic degradation reaction and drying treatment on chitosan and a papaya extract, experiments prove that when the fabric anti-wrinkle finishing agent is used for treating the fabric, the anti-wrinkle performance and the color fastness of the fabric can be remarkably improved, and particularly the color fastness to perspiration and acid stain can be remarkably improved.

Compositions generally related to recycled polymers, copolyester products and related methods, are generally described. The compositions may include copolyester products comprising crystallizable polymers and / or copolymers, which may be present in a post-consumable / post-industrial polymeric material. The copolyester products may have characteristics amenable to enzymatic degradation. In some instances, a polymeric feedstock material can include the copolyester product.

The invention discloses hydrogel loaded with repair-promoting and antioxidant peptides as well as a preparation method and application of the hydrogel, and belongs to the technical field of preparation of medical materials. According to the preparation method, firstly, polypeptide with the amino acid sequence of CEYWVKWCGEKGPAGERG is synthesized, and the polypeptide has antioxidant activity and cell proliferation promoting activity and has good structural stability and enzymatic degradation resistance; and loading the polypeptide into the hyaluronic acid hydrogel to obtain the hyaluronic acid hydrogel. The prepared hydrogel loaded with the repair-promoting and antioxidant peptide has excellent injectable performance and cornea spreadability, can enhance the retention of the polypeptide on the ocular surface, effectively solves the technical problem of high administration frequency of the existing preparation, has good cell and tissue compatibility, and has no adverse reaction in vivo.

The invention belongs to the field of gene engineering, and particularly relates to a PU hydrolase UMG-SP1 mutant with high catalytic activity and application thereof. The amino acids at the 126, 173, 202, 364, 302, 172, 178, 89, 129, 298, 79 and 201 sites in the amino acid sequence of the PU depolymerases as shown in SEQ ID NO: 1 are mutated by using structural analysis and site-specific mutagenesis technologies to obtain a plurality of mutants, and the mutants with improved catalytic activity are obtained. Experiments prove that the PU depolymerization mutants improve the degradation capacity of PU plastic by promoting the hydrolysis efficiency of carbamate bonds in PU, and an important degrading enzyme element is provided for developing an efficient and environment-friendly PU plastic enzymatic degradation technology.

To provide formulations for orally administered poorly permeable molecules, which is highly beneficial for patients who require multiple daily doses, by overcoming at least this low permeability to the intestinal membrane and overcoming, for some of these molecules, particularly peptides and proteins, the chemical and physical instability in the gastrointestinal tract, specifically the loss of activity due to the acidic conditions in the stomach, and the enzymatic degradation throughout the intestine.SOLUTION: Applicants have developed a pharmaceutical formulation intended for oral delivery of a synthetic or natural poorly permeable molecule or a salt / solvate thereof having a therapeutic activity. The pharmaceutical formulation can include a synthetic or natural poorly permeable molecule or a salt or solvate thereof in an amount of 0.01 to 10 wt.% of the total weight of the formulation; a lipophilic phase comprising a triglyceride of a fatty acid in an amount of 50 to 80 wt.% of the total weight of the formulation; and at least one lipophilic surfactant comprising a partial ester of a polyol and a fatty acid in an amount of about 10 to 50 wt.% of the total weight of the formulation.SELECTED DRAWING: None

Described herein are novel oligosaccharides of cannabidiol and other cannabinoids and methods of production and uses thereof. The production method includes contacting cannabidiol with UDP-carbohydrate dependent glycosyltransferases (UGT73A class) to produce a cannabinoid-β-D-glycoside, then contacting the product with a cyclomaltodextrin glucanotransferase and a cyclodextrin to elongate the sugar moiety with (1-4)-α-D-glucopyranoside, thereby producing a mixed 0-a-structure in a recombinant host that has an amphiphilic structure as a surfactant solubilizer and different enzymatic degradability of α- and β-D-glucopyranose residues by amylases and β-glycosidases, thereby allowing targeted drug delivery.

The invention provides a controllable photo-thermal-enzymatic degradation porous material as well as a preparation method and application thereof. The controllable photo-thermal-enzymatic degradation porous material is prepared from the following raw materials: an oil phase mixture and a water phase mixture, the oil phase mixture comprises an enzyme sensitive polymer base material, a random heteropolymer (RHP)-enzyme compound, Janus photo-thermal nanoparticles, an internal phase dispersing agent and a solvent; the aqueous phase mixture comprises water, electrolyte and alginate. According to the porous material provided by the invention, the Janus photo-thermal nanoparticles and the RHP-enzyme compound are coupled, under near-infrared light, RHP conformational change can be triggered to release BC lipase, degradation of the porous material can be triggered under the mild condition of 37 DEG C, and the degradation rate within 48 hours can reach 98%.

The invention discloses a novel application of ginkgo biflavone in lung cancer treatment, belongs to the technical field of medicines, and researches show that ginkgo biflavone can target CDC7 and is a novel targeted medicine. It is confirmed for the first time that ginkgo biflavone regulates and controls the thermal stability and enzymatic degradation of ginkgo biflavone through targeting CDC7 kinase (binding free energies-8.3 kcal / mol), directly intervenes in the DNA replication process, and provides a natural drug lead compound for development of a novel CDC7 small-molecule inhibitor. The compound has great significance in preventing and treating malignant tumors, and has a wide prospect in the aspect of preparing medicines for treating lung tumors.

The invention relates to novel urethanases for the enzymatic degradation of polyurethanes and to an enzymatic process for the complete degradation of polyurethanes in defined monomers.

An enzyme includes an amino acid sequence with, at least, a 80% sequence identity to sequence SEQ ID NO: 1, for fumonisin degradation in a sample. A method for fumonisin degradation is based on the incubation of the contaminated sample with an enzyme comprising an amino acid sequence with, at least, a 80% sequence identity to sequence SEQ ID NO: 1.

The invention discloses a method for delaying water softening in the storage period of fresh-cut tomatoes. The method comprises the following steps: pre-cooling tomatoes, and sequentially carrying out cleaning, sterilization and cutting treatment; and then, sequentially putting into a salicylic acid solution and a low-methoxyl pectin solution for soaking treatment. According to the method, non-enzymatic and enzymatic degradation of pectic polysaccharides of cell walls of the fresh-cut tomatoes is inhibited and the respiration of the tomatoes is reduced through step-by-step soaking treatment of salicylic acid and low-ester pectin, and low-temperature storage is performed. Through the combined technology, the problem of texture quality deterioration of softened water and the like of the fresh-cut tomatoes is effectively inhibited, and the shelf life is prolonged. The method is simple to operate, energy-saving and environment-friendly, solves the problems of long treatment time, high cost, low efficiency and poor effect of a traditional texture maintaining technology, is beneficial to improving the market competitiveness of products, and has good economic benefits and application prospects.

The application belongs to the technical field of biological medicine, and specifically discloses preparation and application of self-assembled glycopeptide nanoparticles for mRNA vaccine delivery, wherein the nanoparticles are formed by self-assembly of a glycopeptide carrier, mRNA and manganese ions. In the nanoparticle preparation process, the manganese ions are adsorbed onto tryptophan and histidine through metal chelation, and play an adjuvant role in activating the cGAS-STING pathway after administration. The nanoparticles can effectively adsorb mRNA, can stably exist for 4 weeks at 4 DEG C, protect mRNA from enzymatic degradation, promote intracellular mRNA internalization and lysosome escape, effectively transfect eGFP-mRNA and Fluc-mRNA in DC2.4 and BMDC cells, and effectively target mRNA delivery to lymph nodes, activate the cGAS-STING pathway, effectively activate the specific immune system in a B16F10-OVA prevention model, delay tumor growth, and prolong the survival cycle of mice.