97 results about "Enzymatic degradation" patented technology

Methods, compositions, and systems for steeping, propagation and fermentation, particularly large-scale operations for production of starch and ethanol and fermentation product streams are provided. Addition of mycotoxin mitigating enzymes or microorganisms expressing mycotoxin mitigating enzymes to steeping, propagation, and / or fermentation tanks, and / or to post-fermentation product streams, mitigates mycotoxin levels in fermentation co-products obtained from mycotoxin contaminated feedstocks.

The present disclosure provides variant enzymes for use in the enzymatic degradation of polyethylene terephthalate (PET).

The application discloses a composite degradation preparation for degrading microplastics in wastewater and an application method thereof, and relates to the technical field of wastewater purification. The preparation is prepared by three steps of preparing a rare earth doped titanium-based porous framework, preparing an enzyme-containing bacteria coated precursor solution, and in-situ cross-linking and co-fixing. The framework realizes visible light response and magnetic separation characteristics. The co-fixing of enzyme and bacteria is completed by microcapsule embedding and carrier adsorption cross-linking. The preparation integrates the functions of photocatalysis, enzymatic degradation and microbial degradation. In application, the wastewater is pretreated to SS≤60mg / L, and then the preparation is added to a degradation tank. Under the conditions of 395-450nm light, 25-40 DEG C, and pH 6.0-8.0, the wastewater is degraded by stage light irradiation. Finally, the preparation is recycled by magnetic separation. The preparation solves the problem of free radical damage in the light-biological synergy, and has strong impact resistance, high microplastic removal rate, and recyclable preparation. The preparation is suitable for continuous treatment of wastewater, greatly reduces the operation cost, and is suitable for purification treatment of various wastewater containing microplastics.

The application belongs to the technical field of bioengineering, and particularly relates to a xanthan gum cleavage ancestral enzyme AncXLY196 based on ancestral sequence reconstruction, an amino acid sequence of which is shown as SEQ ID NO. 2. Based on the xanthan gum cleavage ancestral enzyme, a mutant AncXLY196-X7 is further obtained, an amino acid sequence of which is shown as SEQ ID NO. 4, xanthan gum degradation activity of which reaches 2.5 times of that of the xanthan gum cleavage ancestral enzyme, and the optimal catalytic temperature of the xanthan gum cleavage ancestral enzyme and the mutant thereof is increased to 45 DEG C, and the enzyme activity of the mutant of the xanthan gum cleavage ancestral enzyme is reserved by 50-80% after being treated at 45-55 DEG C for 12-24 h. The application provides a new enzyme resource for efficient enzymatic degradation of xanthan gum and preparation of functional oligosaccharides.

The present invention relates to a process for enzymatic depolymerization of polyethylene terephthalate (PET), in particular contained in a plastic material. The process according to the invention can in particular be implemented on an industrial or semi-industrial scale.

The present invention relates to a process for purifying phycocyanins from a solution comprising phycocyanins and glycogen, said phycocyanins being produced by microorganisms belonging to the order Cyanidiales, characterized in that it comprises (i) an enzymatic degradation step of glycogen with an enzyme suitable for degrading glycogen at a pH below 6 and a reaction temperature below 40°C, said enzyme being selected from a pectinase, a glucoamylase, or a mixture of a pectinase and a pullulanase, and (ii) a step of separating phycocyanins from the glycogen degradation products.

The invention discloses a lotus seed wine brewing method, and belongs to the technical field of wine brewing, selenium-rich lotus seeds without lotus cores are used as a main material, the lotus cores are collected, bitter root sources are eliminated through warm water debitterizing and enzymolysis degradation, the lotus cores and rice husks are compounded and incorporated into an auxiliary material system, the rice husks adsorb residual bitter taste, selenium and flavone of the lotus cores are synchronously reserved, and the lotus seed wine is obtained. Waste utilization of the lotus seeds is facilitated, and beneficial components of the lotus seed wine are improved; in the cleaning link, normal-temperature sterile water containing food-grade oligosaccharide is adopted for low-pressure spraying, the oligosaccharide forms a protective film on the surfaces of the lotus seeds, and selenium dissolution is reduced; a low-pressure low-temperature short-time process is adopted in the cooking link, so that organic selenium and flavone are prevented from being damaged by high temperature; low-temperature sectional interception is adopted for distillation, selenium is prevented from being volatilized along with high-temperature water vapor, and active ingredients are intensively intercepted in a core wine section. According to the invention, the value of the raw materials is maximized, active ingredients are efficiently reserved, the production efficiency and the quality stability are improved, and finally, the wine has drinking experience and health-care properties of lotus seeds.

This invention provides a collagen biphase crosslinked material, its preparation method, and an implantable scaffold. It relates to compositions of polymeric compounds and also to the field of medical materials technology. This application provides a method for preparing a collagen biphase crosslinked material. During the nucleation phase, a low concentration of hydrogen peroxide is introduced to synergistically promote collagen self-assembly and hydrogen peroxide crosslinking. During the growth phase, a higher concentration of hydrogen peroxide solution is added to allow the covalent crosslinked network to form synchronously with fiber extension. The growth phase self-assembly control process can significantly extend the collagen self-assembly time, resulting in more thorough crosslinking and achieving structure-function synergy at the molecular scale. Finally, hydrogen peroxide decomposes into water and oxygen, leaving no crosslinking agent residue. The prepared collagen biphase crosslinked material exhibits good mechanical properties and resistance to enzymatic degradation, and the complete in vivo degradation time can be controlled between 12 and 24 months.

This invention provides a high-recovery heme iron extraction method and apparatus using enzymatic hydrolysis-multi-stage centrifugal coupling, relating to the fields of biopharmaceuticals and fluid dynamics processing technology. The method first constructs a polyanionic electrolyte shielding system, utilizing electrostatic potential energy to induce conformational instability of globin and pre-exposure of hydrophobic cavities, reducing binding energy at the molecular level and solving the problem of heme's encapsulation resistance in high-concentration systems. Then, high-shear coupled enzymatic hydrolysis is performed within a micron-sized liquid film generated by a hypergravity field, utilizing the instantaneous synergy of mechanical shear stress and enzymatic degradation to achieve directional exfoliation of heme, eliminating secondary re-adhesion effects. Finally, directional phase transition regulation is performed based on density gradient drive, and a high-frequency pulsed pressure field is introduced for in-situ exchange washing, distinguishing and retaining impurities and crystal cores, outputting a high-purity, flavor-neutral heme iron product. This invention effectively improves the controllability of the extraction process and the stability of industrial continuous production.

The invention discloses hydrogel loaded with repair-promoting and antioxidant peptides as well as a preparation method and application of the hydrogel, and belongs to the technical field of preparation of medical materials. According to the preparation method, firstly, polypeptide with the amino acid sequence of CEYWVKWCGEKGPAGERG is synthesized, and the polypeptide has antioxidant activity and cell proliferation promoting activity and has good structural stability and enzymatic degradation resistance; and loading the polypeptide into the hyaluronic acid hydrogel to obtain the hyaluronic acid hydrogel. The prepared hydrogel loaded with the repair-promoting and antioxidant peptide has excellent injectable performance and cornea spreadability, can enhance the retention of the polypeptide on the ocular surface, effectively solves the technical problem of high administration frequency of the existing preparation, has good cell and tissue compatibility, and has no adverse reaction in vivo.

The invention belongs to the field of gene engineering, and particularly relates to a PU hydrolase UMG-SP1 mutant with high catalytic activity and application thereof. The amino acids at the 126, 173, 202, 364, 302, 172, 178, 89, 129, 298, 79 and 201 sites in the amino acid sequence of the PU depolymerases as shown in SEQ ID NO: 1 are mutated by using structural analysis and site-specific mutagenesis technologies to obtain a plurality of mutants, and the mutants with improved catalytic activity are obtained. Experiments prove that the PU depolymerization mutants improve the degradation capacity of PU plastic by promoting the hydrolysis efficiency of carbamate bonds in PU, and an important degrading enzyme element is provided for developing an efficient and environment-friendly PU plastic enzymatic degradation technology.

Described herein are novel oligosaccharides of cannabidiol and other cannabinoids and methods of production and uses thereof. The production method includes contacting cannabidiol with UDP-carbohydrate dependent glycosyltransferases (UGT73A class) to produce a cannabinoid-β-D-glycoside, then contacting the product with a cyclomaltodextrin glucanotransferase and a cyclodextrin to elongate the sugar moiety with (1-4)-α-D-glucopyranoside, thereby producing a mixed 0-a-structure in a recombinant host that has an amphiphilic structure as a surfactant solubilizer and different enzymatic degradability of α- and β-D-glucopyranose residues by amylases and β-glycosidases, thereby allowing targeted drug delivery.

The invention provides a controllable photo-thermal-enzymatic degradation porous material as well as a preparation method and application thereof. The controllable photo-thermal-enzymatic degradation porous material is prepared from the following raw materials: an oil phase mixture and a water phase mixture, the oil phase mixture comprises an enzyme sensitive polymer base material, a random heteropolymer (RHP)-enzyme compound, Janus photo-thermal nanoparticles, an internal phase dispersing agent and a solvent; the aqueous phase mixture comprises water, electrolyte and alginate. According to the porous material provided by the invention, the Janus photo-thermal nanoparticles and the RHP-enzyme compound are coupled, under near-infrared light, RHP conformational change can be triggered to release BC lipase, degradation of the porous material can be triggered under the mild condition of 37 DEG C, and the degradation rate within 48 hours can reach 98%.

The invention relates to novel urethanases for the enzymatic degradation of polyurethanes and to an enzymatic process for the complete degradation of polyurethanes in defined monomers.

An enzyme includes an amino acid sequence with, at least, a 80% sequence identity to sequence SEQ ID NO: 1, for fumonisin degradation in a sample. A method for fumonisin degradation is based on the incubation of the contaminated sample with an enzyme comprising an amino acid sequence with, at least, a 80% sequence identity to sequence SEQ ID NO: 1.

The invention relates to the field of soil pollution remediation, and discloses a micro-ecological reconstruction and remediation method for micro-plastic polluted soil, which comprises the following steps: firstly, screening and enriching micro-plastic degradation functional florae which have the advantages of pseudomonas and bacillus, and the bacillus can secrete lipase and protease; the preparation method comprises the following steps: mixing biomass charcoal prepared by pyrolysis of corn straws with a functional bacterium suspension for adsorption, and carrying out sodium alginate-calcium chloride crosslinking to prepare an immobilized composite repairing agent; applying a repairing agent accounting for 1-5% of the mass of the soil, uniformly mixing the repairing agent with the soil, regulating the soil humidity to 20-30% and the carbon-nitrogen ratio to (15-25): 1, and maintaining the environment temperature of 15-35 DEG C; microplastics are removed through the synergistic effect of biomass charcoal adsorption and functional flora enzymatic degradation, beneficial microorganisms are enriched synchronously, and functional gene expression is activated; the problems that in the prior art, functional flora planting is difficult, the degradation efficiency is low, micro-ecological restoration is deficient, and the environmental adaptability is poor are solved.

The present invention provides a GHRH agonist modified with long-chain fatty acylation at the C-terminus. Compared with natural GHRH and existing GHRH agonists, the GHRH agonist modified with long-chain fatty acylation at the C-terminus exhibits significantly improved biological activity and is less susceptible to enzymatic degradation in vivo, thereby showing better stability. The serum half-life exceeds 24 hours, enabling long-acting agonism of GHRH receptors and exerting various therapeutic effects.

The invention discloses a POSTN targeting peptide and a mutant thereof. The POSTN targeting peptide comprises a sequence as shown in SEQ ID NO: 1 and the mutant thereof. The POSTN targeting peptide disclosed by the invention not only has a relatively good blocking effect on POSTN protein, but also has relatively good enzymatic degradation resistance and an effect of inhibiting tumor growth. Moreover, compared with an antibody drug, the POSTN targeting peptide not only has excellent biological activity and biological safety, but also has the advantages of simple structure, easiness in modification and transformation due to carrying of a plurality of functional groups, low synthesis cost and the like.

The invention relates to the technical field of biological catalysis engineering, and discloses a method for preparing enteromorpha oligosaccharide through composite enzymatic hydrolysis gradient alcohol precipitation, which comprises the following steps: in the enzymatic hydrolysis reaction process, establishing negative feedback coupling between stirring power and ethanol concentration increase operation, characterizing the consumption degree of a non-liquid-phase substrate in real time by using the stirring power, and calculating the concentration of the enteromorpha oligosaccharide. According to the present invention, the strong coupling relationship between the chemical reaction and the physical phase change is established, such that the enzymolysis is converted from the random collision process to the physical phase equilibrium-dominated deterministic ordered evolution process so as to achieve the selective precipitation and degradation of the product; and the capability of the system for automatically adapting to the raw material difference is improved, and the technical problems of disordered reaction and poor product selectivity in the existing enzymatic degradation are solved.

The invention discloses a green extraction method of quercetin from kitchen waste pericarp and application thereof, and relates to the technical field of green extraction of natural products, the green extraction method comprises the following steps: S1, a pericarp pretreatment step; s2, preparing an extracting solution; s3, an extraction step; s4, a solid-liquid separation step; s5, an enrichment and purification step; according to the method disclosed by the invention, the dissolution efficiency and selectivity of the quercetin from the pericarp matrix are remarkably improved through the synergistic effect of enzymatic degradation and the deep eutectic solvent (DES) and assisted by physical energy, so that the recovery rate and the enrichment purity of the target component are improved, the subsequent purification burden is relieved, and the practical value of the extract is enhanced. The quercetin enrichment preparation can obviously inhibit the activation of an NF-kappa B signal channel and reduce the expression of downstream proinflammatory cytokines under a non-lethal administration concentration, so that the quercetin enrichment preparation shows the effects of reducing the activation of microglial cells and inhibiting neuroinflammatory response in an in-vitro cell model and an animal neuroinflammatory model; the pathological process related to neuroinflammation can be relieved or prevented.

The invention discloses an immobilized urease catalytic ultrafiltration membrane for degrading urea in yellow wine and a preparation method of the immobilized urease catalytic ultrafiltration membrane, and belongs to the technical field of crossing of polymer separation membranes and enzyme engineering. According to the invention, urease is stably fixed on the inner wall of the membrane hole and / or the membrane surface of the ultrafiltration membrane in a covalent bonding manner, so that the integrated catalytic separation membrane is constructed. The preparation method of the membrane comprises the following steps: performing surface amination modification on a polysulfone (PSF) matrix membrane, and further realizing covalent immobilization of urease through amidation reaction. When the prepared catalytic ultrafiltration membrane is used for treating yellow rice wine, interception of macromolecular impurities and enzymatic degradation of micromolecular urea can be synchronously completed, the urea removal rate can reach 80% or above, enzyme immobilization is firm, no dissolution risk exists, the process is simple and efficient, and a brand new solution is provided for controlling generation of ethyl carbamate (EC) in the yellow rice wine from the source.

The present application relates to the technical field of biological catalysis engineering, and discloses a method for preparing Enteromorpha oligosaccharides by composite enzymolysis gradient alcohol precipitation, which comprises the following steps: in the process of enzymolysis reaction, a negative feedback coupling between stirring power and the increase operation of ethanol concentration is established, the stirring power is used to represent the consumption degree of non-liquid substrate in real time, and the increase of ethanol is triggered on the basis, so that the phase equilibrium is dynamically regulated, and the selective precipitation and degradation of products are realized. By establishing a strong coupling relationship between chemical reaction and physical phase change, the present application changes the random collision process of enzymolysis into a deterministic and orderly evolution process dominated by physical phase equilibrium, and improves the ability of the system to adapt to the differences in raw materials, thereby solving the technical problems of reaction disorder and poor product selectivity in the existing enzymatic degradation.

The present disclosure provides variant enzymes for use in the enzymatic degradation of polyethylene terephthalate (PET).

This invention discloses a BhrPETase keratinase mutant and its application in the degradation of polyethylene terephthalate (PET), belonging to the fields of environmental science and enzyme engineering. The site numbers are based on the amino acid sequence shown in SEQ ID NO:1. The mutant contains substitutions of S14G, H78Y, and S184G relative to SEQ ID NO:1, with the preferred amino acid sequence shown in SEQ ID NO:9. Under conditions of 1 mg enzyme protein / g substrate, 65°C, and 96 h, the preferred mutant exhibits a degradation rate of 90.4% for PET membranes and a half-life of 150 h at 60°C, making it suitable for enzymatic degradation and depolymerization of PET.

A C-terminal truncated alginate lyase and its application. This invention belongs to the fields of bioengineering, genetic engineering, and enzyme engineering, and relates to a C-terminal truncated alginate lyase TY2 designed with molecular docking assistance, its encoding gene, recombinant vector, strain, and its application in the preparation of low-polymerization degree alginate oligosaccharides. Using wild-type MhAly6 as the parent, the truncation boundary was determined through structural prediction and molecular docking. A portion of the linker peptide was retained, and the C-terminal GH28 family domain was deleted to obtain the truncated form TY2. TY2 has approximately twice the specific activity of the wild-type enzyme, and even higher residual activity after incubation at 45°C for 1 hour, achieving a synergistic improvement in catalytic activity and thermal stability. + The relative enzyme activity reached 4.3 times that of the control group, which can degrade pretreated Sargassum powder. The product is mainly ΔDP2–ΔDP4 low-polymerization brown algae oligosaccharides, which are suitable for related enzymatic degradation and oligosaccharide preparation.

The present disclosure relates to a medical tissue adhesive for pancreatic plugging and the preparation method and use thereof. The medical tissue adhesive composition forming the medical tissue adhesive of the present disclosure comprises a medical tissue adhesive component and a chelating agent component; wherein the medical tissue adhesive component comprises a first component and a second component, the first component comprises four-arm polyethylene glycol amine, and the second component comprises four-arm-polyethylene glycol-succinimidyl glutarate; wherein m and n are natural numbers; and the chelating agent component comprises polyphenol. The medical tissue adhesive prepared from the medical tissue adhesive composition of the present disclosure is capable of firmly binding to the surface of the pancreatic tissue, and is capable of withstanding the dynamic pressure as a result of pancreatic juice leakage and the enzymatic degradation activity of pancreatic juice. More importantly, the medical tissue adhesive of the present disclosure has an excellent antibacterial ability and is capable of preventing the infection caused by the long-term accumulation of the leaked pancreatic juice.

The embodiment of the invention provides a connecting unit for chemical modification of a nucleic acid terminal group, a solid-phase carrier and a preparation method of the solid-phase carrier, the chemical modification comprises N-acetylgalactosamine, and the method comprises the following steps: mixing L-deoxyuridine with iodine, and carrying out iodine substitution reaction to obtain 8-iodine-L-deoxyuridine; carrying out triphenyl methylation on the 8-iodine-L-deoxyuridine to protect 5 '-terminal hydroxyl, carrying out palladium catalytic coupling reaction to obtain 8-alkenyl-L-deoxyuridine ester compounds, and hydrolyzing to obtain unsaturated carboxyl modified L-deoxyuridine derivatives; the unsaturated carboxyl modified L-deoxyuridine derivative and N-acetylgalactosamine are subjected to condensation, and the connecting unit for nucleic acid end group chemical modification is obtained. The connection unit obtained by the method has better enzymolysis resistance, and the synthesis method is simple and efficient.

The invention relates to the technical field of detergents, and provides a compound enzyme mildew-removing cleaning tablet as well as a preparation method and application thereof. The cleaning tablet comprises a quick release layer and a slow release layer which are sequentially arranged in a stacked mode. The quick release layer comprises an oxidation component and an effervescent component; the slow-release layer comprises enzyme microcapsules and a surface modification component; wherein the surface modification component is used for depositing on the cleaned hard surface to form the protective film. The enzyme micro-capsule is used for delaying release of an enzymatic degradation mould biological film, the surface modification component is deposited on a hard surface after cleaning to form a protective film, and dirt is prevented from being attached again; through the synergistic effect of the quick release layer and the slow release layer, in combination with chemical oxidation quick sterilization, enzyme microcapsule targeted degradation of a biological membrane and effervescent components, dispersion is promoted, mold can be removed, and a formed protective film is used for preventing dirt from being attached again, so that lasting mold removal is realized, and the recurrence rate is reduced.

This invention belongs to the field of microbial technology, and discloses a highly efficient anaerobic fungal strain for degrading lignocellulose in the rumen of Dulong cattle, its cultivation method, and its applications. The strain is *Neisseria gonorrhoeae*. Joblinomyces apicalis DLM2, deposited at the China Center for Type Culture Collection (CCTCC) under accession number CCTCC NO: M 20252927, was isolated from the rumen of Dulong cattle and can be stably cultured under strictly anaerobic conditions. This strain possesses a single-centered cell line and filamentous rhizoids, enabling it to efficiently degrade lignocellulose through a synergistic effect of physical colonization and enzymatic degradation. It exhibits broad adaptability to natural straws such as rice straw and bamboo branches, as well as purified substrates such as microcrystalline cellulose and cellobiose. Its enzyme production exhibits substrate-inducible properties, and fermentation produces various metabolites including formic acid, acetic acid, and ethanol, demonstrating high lignocellulose degradation efficiency. This invention relates to an inoculum containing this strain and its application in lignocellulose degradation, roughage fermentation, agricultural straw resource utilization, and biomass conversion, providing new strain resources and technical support for the efficient degradation and resource utilization of lignocellulose.