31results about How to "Achieve low toxicity" patented technology

The invention discloses a method for efficiently carrying CRISPR/Cas9 through functionalized graphene oxide for gene editing. An NGO-PEG-PEI/Cas9/sgRNA complex is obtained by the following steps: covalently modifying aminated PEG and aminated PEI onto graphene oxide to obtain NGO-PEG-PEI; then assembling Cas9 protein and sgRNA into a Cas9/sgRNA complex under a room-temperature condition; and mixing NGO-PEG-PEI with the Cas9/sgRNA complex under a room-temperature condition. The editing of target gene can be realized by mixing the complex with the target gene. The functionalized GO has the characteristics of good biocompatibility and high carrying efficiency, can efficiently carry the Cas9/sgRNA complex into cells for performing corresponding functions, and has the effect of keeping the Cas9/sgRNA complex free of enzymatic degradation and high in stability.

The invention discloses a chlorpyrifos-containing insect disinfestation composition, which is mainly characterized by comprising an effective active component chlorpyrifos and an effective component B, wherein the effective component B is selected from thiamethoxam, clothianidin and thiacloprid, a weight ratio of the chlorpyrifos to the effective component B is 20:1-1:5, and the composition can be prepared into a microcapsule suspension and a microcapsule suspension-suspending agent. The composition can be provided for prevention and control of a plurality of insect pests, has characteristics of significant synergy effect and prevention and control spectrum expanding, provides high activities for pests on grassy crops, tea trees, vegetables, cotton and fruit trees, and further has advantages of slow release, pesticide amount reduction, safety on human and animals, no pesticide injury, good environment compatibility, strong preparation adhesion, rain erosion resistance, long lasting period and the like.

The invention relates to preparation and application of a gene and chemotherapeutic drug co-delivery anti-tumor nanoparticle of a target SR-BI (Scavenger Receptor-BI). The nanoparticle is a recombinant high density lipoprotein drug-loaded nanoparticle, comprises phospholipid, cholesterol, cholesteryl ester, apolipoprotein, cation lipid, a chemotherapeutic drug, a gene drug and the like, and is prepared by a film dispersion method. The invention aims at preparing the bionic tumor target drug-loaded nanoparticle, the preparation condition is mild, and the cost is low. The invention further provides the application of the gene and chemotherapeutic drug co-delivery anti-tumor nanoparticle. The gene and chemotherapeutic drug co-delivery anti-tumor nanoparticle has the advantages of spherical structure, good encapsulation efficiency, low cytotoxicity, good targeting, high safety, significant in-vitro anti-tumor effect and the like.

The invention discloses a high-efficiency environment-friendly pesticide complex composition with a preparation form of microcapsule suspension preparation or microcapsule suspension-suspension preparation. The composition comprises, by weight, 0.5-6% of avermectin, 3-40% of spirodiclofen, and balance of auxiliary agents and a filling material. With the pesticide provided by the invention, various mite infestations can be controlled. The pesticide has the advantages of environment friendliness, sustained release, long effect duration, enhanced adhesion force, enhanced penetration, and no phytotoxicity.

The invention discloses a pesticidal suspended seed coating capable of preventing diseases. The seed coating is mainly characterized in that the effective active components are difenoconazole and an effective component B, wherein the weight ratio of the difenoconazole to the effective component B is 1:80-80:1, and the composition is prepared into a suspended seed coating. The invention has the advantages of favorable film formation property, uniform coating, high suspension percentage, high safety, low toxicity and the like, and can be widely used for seed treatment of wheat, corns, paddy rice, peanuts, cotton and other crops. The invention has a slow-release action, reduces the pesticide consumption, is safe for human and livestock, does not have chemical injury, and has favorable environmental compatibility.

The invention discloses a pesticide composition containing sedaxane. Effective active ingredients of the composition consist of sedaxane, tebuconazole and an effective ingredient C, wherein the effective ingredient C is thiamethoxam or imidacloprid, the weight ratio of sedaxane to tebuconazole to the effective ingredient C is (1-80): (1-80): (1-80), and the composition is made into a suspended seed coating. The composition disclosed by the invention can be used for preventing and treating a variety of underground pests and harmful bacteria and has an obvious synergistic effect, the control spectrum is enlarged, and the composition has relatively high activity to pests and harmful bacteria on wheat, vegetables, cotton, peanuts, watermelon and rice; the pests consist of aphids, midge, root-knot nematodes, wireworms, grubs, cutworms, thrips, mole crickets, athetis lepigone and borers, and diseases consist of sheath blight, bakanae disease, gummy stem blight, rust disease, stripe rust, root rot, head blight, powdery mildew, anthracnose, head smut and full rot. The composition has a sustained-release effect, the dosage of pesticides is reduced, and the composition is safe to human and livestock, is free from damage caused by chemicals and is good in environmental compatibility.

The invention discloses a prochloraz-containing pesticide composition. The composition is mainly characterized in that the effective active components are prochloraz and an effective component B, wherein the effective ingredient B is selected from thiamethoxam or clothianidin or thiacloprid; the weight ratio of the prochloraz to the effective component B is 1:100-50:1; and the composition is prepared into a suspended seed coating. The composition disclosed by the invention can prevent and treat many soil pests and germs, has an obvious synergistic action, widens the prevention and treatment spectrum, and has higher activity for pests and germs on wheat, vegetables, cotton, peanuts, cucumbers and paddy rice; and the diseases of paddy rice and vegetables comprise bakanae disease of rice, rice blast, sclerotinia rot of rape, anthracnose of rape, gray mold of cucumber and anthracnose of cucumber. The composition has a slow-release action, reduces the pesticide consumption, is safe for human and livestock, does not have chemical injury, and has favorable environmental compatibility.

The invention discloses a pesticide composition containing carboxin. The composition is mainly characterized in that effective active constituents of the composition comprise carboxin and effective component B, the effective component B is selected from the group consisting of thiamethoxam, thiacloprid, imidacloprid and clothianidin, a weight ratio of carboxin to the effective component B is 1: 80 to 40: 1, and a suspended seed coating agent is prepared from the composition. The composition provided by the invention can be used for preventing and treating a variety of soil pests and pathogens, produces an obvious synergistic effect, has an expanded prevention and treatment spectrum and shows high activity to pests and pathogens on wheat, corn, sorghum and cotton, wherein wheat, corn, sorghum and cotton diseases include corn head smut, wheat smut, wheat rust, millet smut, loose kernel smut of sorghum, head smut, damping-off of cotton and cotton verticillium wilt; furthermore, the composition has a sustained release effect, reduces the dosage of a pesticide, is safe to people and livestock, produces no phytotoxicity and has good environmental compatibility.

The invention discloses a preparation method of a flame-retardant high-performance flexible explosion-proof coating, which comprises a step 1, preparing a component A: modifying isocyanate, polyether resin and phthalic anhydride polymer polyol in an inert environment, namely dehydrating the isocyanate, the polyether resin and the phthalic anhydride polymer polyol at 100-110 DEG C under the vacuum of -0.1 MPa for 2-3 hours, relieving the vacuum, cooling to below 60 DEG C, adding isocyanate, reacting at 85-95 DEG C for 1.5-2 hours, measuring the -NCO value after the reaction is finished, discharging, and filtering to obtain the flame-retardant modified component A. An epoxy group and an isocyanate group are introduced into the coating prepared by the preparation method disclosed by the invention. The heat resistance of the coating is further improved on the basis that the epoxy has the advantages of good processing property, excellent mechanical property, interface property and the like. Polyether amine reacts with epoxy and isocyanate, polyether and the reactive flame retardant react with isocyanate to form a multi-dimensional network structure in a system, so that the excellent flame-retardant and explosion-proof performance of the coating is ensured.

The invention discloses an insecticide combination containing pymetrozine and high-efficacy lambda-cyhalothrin, which is mainly characterized in that the insecticide combination contains active ingredients, i.e. the pymetrozine and the high-efficacy lambda-cyhalothrin, the remaining ingredients are assistants, the weight ratio of the pymetrozine to the high-efficacy lambda-cyhalothrin is 9:1 to 1:9, and the combination is made into microcapsule suspension, microcapsule suspension - suspension or oil suspension. The insecticide combination has the advantages that the insecticide combination can prevent and control various injurious insects, the synergistic effect is obvious, the insecticidal spectrum is expanded and the activities to the injurious insects on rice, vegetables, cotton and fruit trees are high; the insecticide combination has a slow release effect, the pesticide dosage is reduced, the insecticide combination is safe to human beings and livestock, the pesticide damage does not exist and the environmental compatibility is good; and the preparation adhesive force is reinforced, the insecticide combination is resistant to rainwater washing, the efficacy duration is long, and the like.

The invention discloses an electrical cooling, arc-extinguishing and fire-extinguishing reagent and a preparation method thereof, and the electrical cooling, arc-extinguishing and fire-extinguishing reagent comprises the following substances in parts by mass: 50-70 parts of perfluorohexanone, 30-50 parts of an organic silicon flame retardant, 20-40 parts of 5, 5-dimethyl-2-chloro-1, 3, 2-dioxo-phosphorus hetero-caprolactone phosphate, 5-12 parts of metal hydroxide, 20-30 parts of graphite powder and 3-5 parts of a dispersing agent. According to the fire extinguishing agent disclosed by the invention, the perfluorohexanone is adopted as a fire extinguishing agent, and the organic silicon flame retardant, the 5, 5-dimethyl-2-chloro-1, 3, 2-dioxo-phosphorus hetero-caprolactone phosphate, the inorganic flame retardant metal hydroxide and the graphite powder with a porous structure are supplemented, so that the problem that after-combustion is easy to occur when the perfluorohexanone fire extinguishing agent is independently applied is solved; and the porous structure of the graphite powder can adsorb HF toxic gas generated in the fire extinguishing process of the perfluorohexanone, so that air pollution is reduced.

The invention discloses a preparation method of a nitrophenylacetonitrile compound. The nitrophenylacetonitrile compound is shown as a structural formula (1), wherein R1 refers to hydrogen, alkyl with 1-6 carbon atoms or halogenated alkyl with 1-3 carbon atoms; and R2 refers to hydrogen or alkoxyl with 1-6 carbon atoms. The method comprises the following step of: reacting the compound shown as a structural formula (2) with ferrous iron cyanide salt in a solvent under the action of cuprous chloride and/or cuprous bromide serving as a catalyst under a heating condition, wherein the definitions of R1 and R2 in the formula (2) are the same as those of R1 and R2 in the formula (2). Due to the adoption of the method for preparing nitrophenylacetonitrile by taking an o-nitrochlorobenzene compound as an initiator, the toxicity of a cyanidation reaction and the cost of a reactant can be lowered; meanwhile, cuprous chloride and cuprous bromide are used in a matching way, so that an unexpected effect is achieved, the product yield can be increased, and industrial mass production is facilitated; and moreover, three wastes do not contain virulent substances, and post-treatment is easy.