17 results about "Avermectin" patented technology

The present invention provides an anti-parasitic composition for use in the treatment or prevention of a parasitic infection in a mammal, the anti-parasitic composition comprising a macrolide, in particular a combination of ivermectin, abamectin and doramectin. The invention also provides a method of treating a parasitic infection in a mammal by administering to the mammal an effective amount of an anti-parasitic composition comprising ivermectin, abamectin, and doramectin, and a method of preparing the anti-parasitic composition.

The plant source insecticide contains effective components of parathion and avermectin, the weight ratio of parathion and avermectin is 10-50:1, and is supplemented with an acceptable agricultural adjuvant to form one of emulsifiable concentrate, wettable powder, water dispersible granule, water emulsion or suspension agent suitable for agricultural use, and belongs to the field of pesticides. The plant source insecticide shows activity on peridroma saucia, aphid and cotton red spider, especially high acaricidal activity on red spider. The prevention and treatment effect on citrus red spider is obviously improved after parathion and avermectin are compounded, which shows that the combination of parathion and avermectin has significant synergistic effect on citrus red spider. Parathion is derived from Chinese herbal medicine plant, and there is no problem of pesticide residue, and the risk of drug damage is greatly reduced. At the same time, the two single agents of the composition have great difference in chemical structure and completely different action mechanism, and there is no cross resistance, which can delay the resistance problem caused by the use of the two single agents alone.

The present invention relates to combinations of a compound of formula (A-1) with a compound selected from the group consisting of dimpropyridaz, emamectin and isocycloseram, bentioflumin, pioxaniliprole, isoflualanam, cybenzoxasulfyl, vinylfluthrin, cyclobunofen, flupymezotiaz, fentiazoluron, 1-(1,2-dimethylpropyl)-5-methyl-N-prop-2-ynyl-N-(3-pyridyl)pyrazole-4-carboxamide, 1 -[4- [5-ethylsulfonyl-1-methyl-4-[3-methyl-6-(trifluoromethyl)imidazo[4,5-b]pyridin-2-yl]imidazol-2- yl]phenyl]cyclopropanecarbonitrile, [(Z)-[[3-ethylsulfonyl-5-[4-(trifluoromethoxy)phenyl]-2-pyridyl]-[[5- (trifluoromethyl)-2-pyridyl]amino]methylene]amino] acetate, 3-[3-ethylsulfonyl-5-[4- (trifluoromethoxy)phenyl]-2-pyridyl]-4-[5-(trifluoromethyl)-2-pyridyl]-1,2,4-oxadiazol-5-one, 1-(4- chlorophenyl)-2-fluoro-4-methyl-5-(2,2,2-trifluoroethylsulfanyl)benzene, and emamectin benzoate; and to methods of controlling or preventing damage to plants using such combinations; and to formulations, concentrates and seed treatments comprising such combinations.

The present invention belongs to the technical field of pesticides, and specifically relates to an insecticidal composition containing 4-(trifluoromethyl)nicotinamide, and an application thereof. The insecticidal composition includes an active ingredient A and an active ingredient B, wherein the active ingredient A is 4-(trifluoromethyl)nicotinamide, and the active ingredient B is selected from one or more of the following compounds: afidopyropen, formula (I), dimpropyridaz, formula (II), isoflualanam, fipronil, imidacloprid, thiamethoxam, avermectin, acephate and beta-cypermethrin. The composition has features such as reasonable components, good insecticidal effect, low administration cost, reduced application amount, crop safety, and reduced residue.

The invention discloses a preparation method of an eprinomectin intermediate oxide. The eprinomectin intermediate oxide is obtained by taking an abamectin hydroxyl protection substance as a starting raw material through oxidation reaction and post-treatment. According to the invention, active manganese dioxide is adopted as an oxidizing reagent, the operation is simple, the generation of peculiar smell of a by-product dimethyl sulfide during dimethyl sulfoxide oxidation in the prior art is avoided, the yield reaches 98.0% or more, the purity of the oxide can reach 95.0% or more, and the content of double-oxide impurities can be controlled to be 0.2% or less.

The invention belongs to the technical field of genetic engineering and metabolic engineering, and particularly relates to a streptomyces avermitilis double promoter and application thereof. The double promoter ermEp *-P7983 provided by the invention is formed by connecting a promoter ermEp * and a promoter P7983 in series, and the nucleotide sequence of the double promoter ermEp *-P7983 is as shown in SEQ ID NO. 2. The double promoter has higher activity, and can significantly improve the expression level of a target gene in abamectin and increase the yield of a target metabolite, so that the double promoter can be used for constructing a recombinant plasmid for highly expressing the target gene and a streptomyces avermitilis recombinant bacterium, and has broad application prospects. And the constructed streptomyces avermitilis recombinant strain shows the activity of efficiently synthesizing a target product in different culture media, and has stronger adaptability.

The present application relates to a synergistic composition containing a biologically effective amount of compound A and component B, wherein the component B is one of thiazide ketone, avermectin, spirotetramat, pyridalyl, emamectin benzoate, azamethiphos, azocyclotin, pyridaben, quimacryd thiocyl, pyrimidifen, tebufenpyrad, flufenoxuron, chlorfenapyr, cyromazine, and halofenozide, and a method thereof for controlling pests in agronomic and non-agronomic environments. The compound A has a synergistic effect with the component B, which can improve the pest control effect, reduce the application amount, reduce the use frequency, and reduce the use cost.

The present application belongs to the technical field of pesticides, and relates to a pesticide composition for preventing and treating nematodes. Effective components are avermectin and ethylicin, and the mass ratio of the avermectin to the ethylicin is 1:5-1:29. The present application has obvious synergistic effect on preventing and treating root-knot nematodes from invading plant root systems by compounding avermectin and ethylicin as effective components, and this effect has not been reported. The preparation product containing the composition can improve the control effect on root-knot nematodes and has development potential. The action mechanisms of the avermectin and the ethylicin are different, the persistence period is long, the use frequency can be reduced, and the generation of drug resistance can be delayed. The present application is mainly used for preventing and treating nematodes of solanaceae vegetables, cucurbitaceae vegetables, lettuce and other crops.

The invention provides a method for improving the proportion of an effective component A of avilamycin by enhancing component conversion. Specifically, the invention provides a method for improving the proportion and / or yield of an effective component A of avilamycin, and the method comprises the following steps: providing an avilamycin production strain, enhancing the expression of AviO2, AviB1 and / or AviB2 proteins in the avilamycin production strain or increasing the copy number of aviO2, aviB1 and / or aviB2 genes in the avilamycin production strain, therefore, the proportion and / or the yield of the component A produced by fermentation of the avilamycin production strain are / is improved. According to the recombinant strain containing the multi-copy aviO2, aviB1 and / or aviB2 genes obtained by the method, the proportion of the effective component A of avilamycin can be increased from the source, so that the extraction process is simplified, the production cost is reduced, the genetic stability is good, and obvious social and economic benefits are achieved.

The invention belongs to the field of synthetic biology and microbial pharmacy, and particularly relates to recombinant streptomyces, a construction method of the recombinant streptomyces and application of the recombinant streptomyces in preparation of savermectin B. According to the present invention, after the aveA1 gene segment in the abamectin biosynthesis gene cluster in the streptomyces avermectin is replaced with the nemA1 gene segment in the nemadectin biosynthesis gene cluster in the streptomyces blue griseolus, the recombinant streptomyces capable of producing the 16-membered macrolide compound-Sesvermectin B (Senvermectin B), such as the recombinant streptomyces avermectin HU520-M provided by the invention, is obtained; experiments show that the savermectin B has efficient insecticidal, acaricidal and anti-parasitic activity, and shows good industrial development potential.

The invention relates to a method for preparing abamectin fermentation liquor by biologically fermenting a contaminated material. The method comprises the following steps: three-stage fermentation culture which is divided into first-stage seed culture, second-stage seed culture and fermentation tank culture, preparing abamectin fermentation liquor by utilizing a fermented bacterial material, mixing rape seed cake powder and wheat germ powder according to a certain proportion, and fermenting by combining a precursor fed-batch feeding method, so that the effect of recycling energy is achieved; the environmental protection pressure is relieved, the fermentation titer is promoted to be improved, the fermentation period is shortened, and the drinking water consumption is reduced.

This invention relates to a method for increasing the yield of polyketide compounds by splitting a modular polyketide synthase gene. (1) Constructing a RedEx gene cassette for splitting the modular polyketide synthase gene; (2) Constructing a target plasmid carrying an amp-ccdB gene selection expression cassette; (3) Constructing a target plasmid carrying an amp-ccdB gene selection expression cassette. C DD-TGA-RBS-ATG- N (3) Target plasmid encoding the DD coding sequence; (4) Construction of recombinant strains; (5) Fermentation culture to obtain polyketide compounds. This invention is the first to discover that the biosynthetic efficiency of host strains carrying the split PKS gene is much higher than that of host strains carrying the natural PKS gene, which can achieve the purpose of increasing the yield of polyketide compounds. Using this method of splitting modular polyketide synthase genes, high-yield butenyl spinosad genetically engineered bacteria and high-yield avermectin genetically engineered bacteria were successfully constructed, which effectively increased the yield of butenyl spinosad and avermectin. The yield of butenyl spinosad increased by a maximum of 30 times, and the yield of avermectin increased by a maximum of 5.8 times.

The invention discloses a preparation method of avilamycin fermentation liquor, and belongs to the technical field of microbial fermentation. According to the method, streptomyces viridochromogenes is used as a strain, a fermentation production culture medium with a specific formula is adopted for fermentation, the fermentation process is precisely controlled through feeding by a flow addition method, and finally the avilamycin fermentation liquor is prepared. By optimizing a culture medium formula and a fermentation process, the fermentation titer of avilamycin reaches more than 6500u / ml, the fermentation time is controlled within 200h, the problems of low fermentation yield and high energy consumption in the prior art are effectively solved, and the method is suitable for industrial production.

The application discloses a streptomyces avermitilis-purpureococcus texanus compound microbial agent and a preparation device and method thereof, which comprises a strain preparation module, a culture medium configuration module, a compound fermentation module and a centrifugal purification module, wherein the compound fermentation module comprises a fermentation tank, a detection mechanism for detecting the compound microbial agent, a control mechanism for controlling the detection time interval and a cleaning mechanism; the detection mechanism comprises a detection rack arranged in the fermentation tank in a lifting mode, a plurality of detection barrels fixed on the detection rack, a protective barrel rotatably arranged outside the detection barrel, a pH sensor arranged in the detection barrel and a driving assembly for controlling the rotation of the protective barrel, the detection barrel is provided with a detection hole, the protective barrel is provided with a protective hole, and the inner wall of the protective barrel blocks the detection hole when the protective barrel is in an initial position; and the cleaning mechanism is used for cleaning the pH sensor. The compound microorganism in the application combines the respective advantages of the three kinds of microorganisms and can more widely prevent and control various pests, nematodes and pathogenic bacteria.

The application discloses a doramectin monoclonal antibody, a detection test strip and application thereof, and relates to the field of animal health. 50 The heavy chain and light chain variable regions of the monoclonal antibody have specific complementarity determining region sequences, and the half inhibition concentration IC of the monoclonal antibody to doramectin is as low as 3-5 ng / mL, which shows excellent sensitivity; the cross reaction rates of the monoclonal antibody to abamectin, ivermectin and acetyl abamectin are 75%, 75% and 60% respectively, and the monoclonal antibody has no cross reaction with common antibiotics such as tetracycline and gentamicin. Based on the antibody, the application also develops a colloidal gold immunochromatography test strip, which is simple in operation, rapid in detection, visual in result, and has a detection limit of 5 ng / mL, and is suitable for on-site rapid screening of doramectin and abamectin drug residues in animal-derived food, and has a good application prospect.

The present invention relates to stable synergistic insecticidal composition for synergistic and effective control of various insect pests including entire sucking pest complex.

A mixture for killing pests, comprising, as the active compound, A) an ethyl sulfone compound having formula (I) and B) At least one additional compound B, selected from fipronil, λ-cyhalothrin, bifenthrin, heptafluthrin, α-cyhalothrin, thiamethoxam, thiamethoxam, acetamiprid, imidacloprid, dinotefuran, trifluralin, dithiamethoxam, spinosad, ethyl spinosad, abamectin, bispyribac-methyl, indoxacarb; cyfluthrin, methoxypiperazine ethyl ester, dispirenil, chlorantraniliprole, brofenoxuronamide, tetrazolium acetonitrile, cyclobromhizobium acetonitrile, brofenoxuron dimethoate; pyrimethanil, M.UN.1: 1-[6-(2,2-difluoro-7-methyl-[1,3]m-dioxacyclopenten[4,5-f]benzimidazol-6-yl)-5-ethylsulfonyl-3-pyridyl]cyclopropaneformonium, M.UN.2: 6-(5-cyclopropyl-3-ethylsulfonyl- 2-pyridyl)-2,2-difluoro-7-methyl-[1,3]m-dioxacyclopenten[4,5-f]benzimidazole; and difenoconazole, prothioconazole, fenbendazole, tebuconazole, chlorfluazuron, cymoxanil, fluopyram, tetrazoxystrobin, azoxystrobin, oxadiazon, azoxystrobin, fluopyram, fluopyram, fluopyram, fluopyram, fluopyram, isopropoxystrobin, trifluoropyridamole, fluthiazolinone, flusaprolin, metalaxyl, tetrazoxystrobin, fludioxonil, thiophanate-methyl; these mixtures are used to combat invertebrate pests such as insects, arachnoides or nematodes inside and on the surface of plants, and for the protection of such plants, especially for the protection of plant propagation material such as seeds from pest infestation.