33 results about "Stevia <genus>" patented technology

A stevia cultivar with super high Rebaudioside A content, designated ‘320032’, is disclosed. Another embodiment relates to the plant parts of stevia cultivar ‘320032’, to the plants of stevia ‘320032’ and to methods for producing a stevia plant produced by crossing the cultivar ‘320032’ with itself or another stevia variety, including methods using marker assisted breeding. Another embodiment further relates to hybrid stevia seeds and plants produced by crossing the cultivar ‘320032’ with another stevia cultivar. Twelve highly polymorphic SNPs loci and the corresponding genomic sequences used to identify plant variety ‘320032’-derived plant materials are also disclosed.

Provided is a stevia plant having at least one of genetic characteristics (1) to (5).

The present invention provides a Stevia plant having a low ability to form pollens as compared with the wild type Stevia species. The present invention also provides a method of producing such a Stevia plant having a low ability to form pollens, and an extract or a steviol glycoside purified product obtainable from such a plant.

A Stevia rebaudiana residue fermented feed additive for improving livestock and poultry productivity, a preparation method therefor, and a use thereof. Raw materials of the Stevia rebaudiana residue fermented feed additive comprise: Stevia rebaudiana residue, fermented brown sugar, a microbial agent, and water. The microbial agent is composed of EM bacteria, white rot fungus and Phanerochaete chrysosporium, in a ratio of 1:1:1.

The invention relates to the technical field of biological control, and discloses an application of steviosin or a substance containing the steviosin in preparation of a nematode control product. According to a caenorhabditis elegans toxicity tracking method, stevia rebaudiana mother liquor sugar is separated according to the polarity of the substance; two compounds are separated and purified from mother liquor sugar by using normal phase silica gel, reverse phase silica gel, LH20 gel and liquid chromatography in sequence, the compounds are identified by using mass spectrum and nuclear magnetism, the compounds are determined to be Steretin F and Steretin E, the Steretin F and the Steretin E are proved to have nematicidal toxicity, the Steretin F has the strongest toxicity, LC50 is 1.57, the toxicity effect is stronger than that of thiazoline and weaker than that of abamectin, and the Steretin F and the Steretin E have the nematicidal toxicity. A new direction is provided for application of stevia rebaudiana, and an important theoretical and practical basis is provided for development and large-scale application of natural insecticidal compounds.

The utility model relates to the technical field of plant cultivation frames, and discloses a stevia rebaudiana hybridization net rack, which comprises a frame and a plurality of support columns, the inside of the frame is fixedly connected with a segmentation net rack, the inside of the frame is fixedly connected with a plurality of mounting plates, the inside of each mounting plate is slidably connected with two arc clamps, and the arc clamps are fixedly connected with the frame. A convex button is fixedly connected to the rear side of the arc clamp, a plurality of motors are fixedly connected to the interior of the frame, the driving end of each motor is fixedly connected with a rotating disc, an arc groove is formed in the outer portion of each rotating disc, and a plurality of recording assemblies used for containing recording cards are arranged on the outer portion of the frame; a telescopic assembly used for adjusting the height of the net rack is arranged in the supporting column. According to the utility model, the motor drives the rotating disc to rotate, so that the two arc clamps are driven to slide towards the close sides, male flowers and female flowers of stevia rebaudiana to be hybridized lean against each other, the workload of artificial pollination is reduced, and the working efficiency is improved.

The invention belongs to the technical field of feed additive preparation, and particularly discloses a fermented stevia rebaudiana composition and application thereof in preparation of a feed additive. The fermented stevia rebaudiana composition is prepared from the following components in parts by weight: 25-35 parts of stevia rebaudiana, 12-14 parts of corn straw, 10-30 parts of soybean meal, 2-3 parts of honeysuckle, 6-8 parts of Chinese yam, 1-2 parts of hawthorn, 4-7 parts of bran and 1-2 parts of dandelion. The invention discloses a fermented stevia rebaudiana composition and application thereof in preparation of a feed additive, the fermented stevia rebaudiana composition has high content of total flavonoids and total phenols, when applied to laying hen breeding, the fermented stevia rebaudiana composition can significantly enhance the oxidation resistance of organisms and effectively inhibit inflammatory response, and is beneficial to the health of reproductive systems; therefore, the production performance of laying hens is obviously improved and the egg quality is improved.

A stevia cultivar containing a high Rebaudioside M content, designated ‘18136109’, is disclosed. The invention relates to the plant parts of stevia cultivar ‘18136109’, to the plants of stevia ‘18136109’ and to methods for producing a stevia plant produced by crossing the cultivar ‘18136109’ with itself or another stevia variety, including methods using marker assisted breeding. The invention further relates to hybrid stevia seeds and plants produced by crossing the cultivar ‘18136109’ with another stevia cultivar. Twelve highly polymorphic SNPs loci and the corresponding genomic sequences used to identify plant variety ‘18136109’-derived plant materials are also disclosed.

The application discloses a method for improving the germination rate of stevia seed, and specifically comprises the following steps: selecting full and healthy stevia seed with consistent size for standby; disinfecting the stevia seed with 10% sodium hypochlorite solution for 5-15 minutes, then rinsing with distilled water for multiple times and filtering dry; preparing different concentrations of salicylic acid to pretreat the stevia seed; taking equal amounts of salicylic acid with different concentrations into at least three portions, and adding equal amounts of the disinfected stevia seed into each portion for seed soaking; after the seed soaking, rinsing with distilled water for 3-5 times, and performing seed germination test by adopting the culture dish filter paper method; starting observation and recording from the day when the stevia seed is placed on the bed; and comparing the germination of the stevia seed. The method for improving the germination rate of stevia seed has the advantages of convenience, high efficiency, non-toxicity, non-residue, low cost, high economic benefit, and wide application prospect in modern efficient agricultural production.

The invention belongs to the technical field of processing and feeding devices, and particularly relates to a stevia rebaudiana processing and feeding device which comprises a waste liquid bin, and a conveying mechanism used for conveying waste residue liquid generated in the stevia rebaudiana processing process is arranged in the waste liquid bin. An auxiliary mechanism used for crushing impurities in stevia rebaudiana waste residue liquid is arranged above the conveying mechanism, the conveying mechanism comprises a rotatable auger, two flow guide plates capable of swinging in a reciprocating mode are arranged above the auger, one end of the auger is fixedly connected with a driving gear, and the other end of the auger is fixedly connected with a driven gear. A plurality of crushing blades are driven by a crushing rod to rotate, so that fiber solid impurities uniformly falling from a guide plate can be crushed and separated in advance, waste residue liquid can be uniformly conveyed by an auger, and the influence of the solid impurities and the like on the conveying effect of the auger can be avoided; the effect of conveying the stevia rebaudiana waste residue liquid to the next procedure can be effectively improved, and subsequent treatment on the waste residue liquid is facilitated.

A method of screening for a stevia plant with high steviol glycoside content, that includes detecting from the genome of a test stevia plant the presence and / or the absence of the following genetic feature: (1) homozygous for the allele wherein the base at the position corresponding to position 290 of SEQ ID NO: 1 is T, and the presence and / or the absence of the following genetic feature: (2) homozygous or heterozygous for the allele wherein the base at the position corresponding to position 40 of SEQ ID NO: 2 is A.

The present invention belongs to the field of plant genetic engineering, and relates to a steviol glycoside glycosyltransferase gene related to steviol glycoside biosynthesis, its encoded product and applications. The gene of the present invention was first cloned from Stevia rebaudiana, and its nucleotide sequence is as shown in SEQ ID NO.2, and the amino acid sequence encoded by it is as shown in SEQ ID NO.1. The steviol glycoside glycosyltransferase of the present invention has the ability to specifically glycosylate the C13 position, but cannot glycosylate the C19 position, and has a higher activity towards substrates with disaccharide glycosylation at the C13 position, and a weaker activity towards glycosides with only monosaccharide at the C13 position. Overexpression of the glycosyltransferase of the present invention in Stevia rebaudiana can provide a new direction for the improvement of Stevia rebaudiana varieties.

The utility model relates to the technical field of stevia rebaudiana production and processing, and discloses a stevia rebaudiana production and processing distributing device which comprises a tank body, a water inlet pipe is fixedly connected to the upper portion of the tank body, a first valve is arranged in the middle of the water inlet pipe, the water inlet pipe penetrates through the top of the tank body, and a top shower is fixedly connected to the bottom of the water inlet pipe. A feeding cover is arranged on the upper portion of the left side of the outer wall of the tank body, a discharging cover is arranged on the lower portion of the right side of the outer wall, a filter screen is arranged on the inner wall of the tank body close to the lower portion, a water outlet pipe is fixedly connected to the bottom of the tank body, a second valve is arranged in the middle of the water outlet pipe, and a side plate is arranged on the right side of the discharging cover. According to the device, purified water is injected into the top shower through the water inlet pipe by opening the first valve, and then the purified water is uniformly sprayed to the surface of stevia rebaudiana on the surface of the filter screen through the top shower, so that dust and soil on the surface of stevia rebaudiana can be efficiently dissolved, and the purpose of reducing waste of water resources and artificial resources is achieved.

The present invention provides a high rebaudioside M content non-genetically modified stevia plant comprising rebaudioside M at higher content as compared with the wild type stevia species. The present invention also provides a method of producing such a high rebaudioside M content non-genetically modified stevia plant, and a dry leaf obtainable from such a plant.

The invention belongs to the field of plant genetic engineering, and relates to a stevioside glycosyl transferase gene related to stevioside biosynthesis as well as a coding product and application of the stevioside glycosyl transferase gene. The gene is cloned from stevia rebaudiana for the first time, the nucleotide sequence of the gene is as shown in SEQ ID NO.2, and the coded amino acid sequence of the gene is as shown in SEQ ID NO.1. The stevioside glycosyl transferase disclosed by the invention has the capability of carrying out specific xylosylation on a C19 site, the capability of carrying out glycosylation on a C13 site is relatively weak, the activity on a substrate with monosaccharide glycosylation on the C13 site is relatively high, and the activity on glucoside containing a plurality of glycosyls on the C13 site is relatively weak. Overexpression of the glycosyl transferase in stevia rebaudiana can provide a new direction for stevia rebaudiana variety improvement.

A Stevia extract made from leaves of the Stevia rebaudiana plant is described. The extract has desired levels of steviol glycosides and is useful in food, beverage, and other consumable products.

The invention discloses a preparation method of an extract for improving the flavor of stevia rebaudiana. Aiming at the flavor defects of leaf flavor, peppery flavor and the like of a stevia rebaudiana extract, the technical scheme comprises the following steps: carrying out colloid embedding on the stevia rebaudiana extract by adopting carrageenan, and combining with drying treatment, so that peculiar smell is effectively removed, characteristic aroma is enhanced, and the flavor level of the stevia rebaudiana extract is optimized; the method is suitable for the fields of food, beverage and health-care products.

The invention relates to the technical field of pet hair repair, in particular to a hair repair composition for pet cats as well as a preparation method and application thereof, and the hair repair composition comprises stevia rebaudiana extract and inositol. The stevia rebaudiana extract and the inositol are mixed according to the mass ratio of 1: (5-15). The preparation method comprises the following steps: (1) respectively weighing the stevia rebaudiana extract and the inositol according to the mass ratio, adding purified water, and stirring and dissolving at 40-60 DEG C to obtain a mixed solution for later use; and (2) drying the mixed solution in the step (1) to obtain powder, namely the hair repairing composition. The hair repairing composition provided by the invention can be used for remarkably improving the hair glossiness, smoothness and elasticity of pet cats.

A method of screening for a stevia plant with high rebaudioside M content ratio, that includes detecting from a test stevia plant the presence and / or absence of at least one of (1) and (2), and at least one of (3) to (7): (1) homozygous for the allele wherein position 290 of SEQ ID NO: 1 is T; (2) homozygous for the allele wherein position 33 of SEQ ID NO: 2 is A; (3) homozygous for the allele wherein position 44 of SEQ ID NO: 3 is T; (4) homozygous for the allele wherein position 40 of SEQ ID NO: 4 is T; (5) homozygous for the allele wherein position 48 of SEQ ID NO: 5 is C; (6) homozygous for the allele wherein positions 55-72 of SEQ ID NO: 6 are deleted; (7) homozygous for the allele wherein position 50 of SEQ ID NO: 7 is A.

The application discloses a preparation method of a stevia extract, and belongs to the technical field of plant extract preparation; the preparation method comprises the following steps: S1, crushing stevia raw materials, then soaking the stevia raw materials in water, adding a composite enzyme to perform enzymolysis, and preparing an enzymolysis solution; S2, performing flocculation treatment on the enzymolysis solution; the flocculating agent is a composite flocculating agent, and a crude extract solution is obtained; S3, performing gradient elution on the crude extract solution after adsorption by using a macroporous resin, and collecting eluate of a specific section; and S4, performing elution after adsorption of the eluate by using an aluminum oxide column, concentrating the eluate, and crystallizing; the preparation method only uses water and ethanol as solvents in the whole process, does not use toxic organic reagents, is simple to operate, and is very suitable for industrial mass production.

The invention relates to the technical field of feed additives, in particular to a chicken feed additive and application thereof, and the chicken feed additive comprises the following components in parts by weight: 20-50 parts of stevia rebaudiana extract, 30-60 parts of scutellaria baicalensis extract, 10-20 parts of montmorillonite and 20-40 parts of stevia rebaudiana waste residue. The preparation method comprises the following steps: sequentially weighing the stevia rebaudiana extract, the scutellaria baicalensis extract, the montmorillonite and the stevia rebaudiana waste residues in parts by weight, and uniformly stirring and mixing. The feed additive is added into a basal feed in parts by weight, can effectively prevent chicken necrotic enteritis, greatly improves the immunity of broiler chickens, and is more beneficial to the growth of the broiler chickens.

The utility model discloses cleaning and impurity filtering equipment for stevia rebaudiana extraction, which belongs to the technical field of cleaning equipment, and comprises a base, one end of the base is fixedly connected with a cleaning box, the bottom end of the cleaning box is provided with a water outlet, the other end of the base is fixedly connected with a support frame, and the top end of the support frame is welded with a movable frame. An electric push rod is bolted to the outer side wall of the moving frame, a push rod part of the electric push rod penetrates through the moving frame and is provided with a moving seat, a stirring assembly is arranged at the top end of the moving seat, a water storage tank is fixedly connected to the upper surface of the base, and a flushing mechanism is arranged at one end of the water storage tank; a filter plate for filtering impurities in water is arranged in an inner cavity of the water storage tank, so that the cleaning speed of the stevia rebaudiana is increased, the influence on the extraction efficiency of the stevia rebaudiana is reduced, the cleaning water of the stevia rebaudiana can be recycled, and the waste of resources is reduced.

The invention belongs to the technical field of agricultural biology, and particularly relates to application of a stevia rebaudiana low-phosphorus response gene PHR family in improving the content of glycoside in stevia rebaudiana. The invention provides application of a stevia rebaudiana low-phosphorus response gene PHR family in improving the content of glycoside in stevia rebaudiana, the stevia rebaudiana low-phosphorus response gene PHR family comprises a SrPHR1 gene and a SrPHR13 gene, and the relative content of rebaudioside C, steviobioside, rebaudioside D and total glycoside is improved by overexpressing the SrPHR1 gene; the relative content of stevioside and rebaudioside A is reduced through overexpression of the SrPHR1 gene; the relative contents of rebaudioside A, rebaudioside C and total glycosides are increased by overexpressing the SrPHR13 gene; the relative content of stevioside and rebaudioside D is reduced through overexpression of the SrPHR13 gene. The invention clarifies the influence of low phosphorus on the yield and quality of stevia rebaudiana, and preliminarily explores the application value of the phosphorus regulatory gene in improving the quality of stevia rebaudiana.

To provide a novel stevia sweetener component.SOLUTION: The component analysis of a novel steviol glycoside component and stevia extract and crystalline products containing them can identify the raw material of a sweetener containing RA as a main component, while making the quality control of sweeteners easy, thereby making the judgment of the appropriateness of the origin labelling of stevia variety and infringement easy. The invention relates to a novel steviol glycoside; a production of a novel steviol glycoside included in a Stevia Rebaudiana Bertoni variety which contains rebaudioside A in large quantities, and a production of a sweetener, food, pharmaceuticals, quasi drugs, and cosmetics containing them; an identification method of stevia variety; and an analytical method of the novel steviol glycoside.SELECTED DRAWING: None

The invention relates to table vinegar containing a natural sweetening agent, which is characterized in that the table vinegar is formed by mixing one liter of wine vinegar and 20-30g of stevia rebaudiana leaves, so that the product is endowed with unique new flavor characteristics. The preparation method comprises the following steps: step 1, mixing the wine vinegar and the stevia rebaudiana leaves according to a mixing proportion that 20-30g of stevia rebaudiana leaves are added into each liter of table vinegar, and filling the mixture into a wooden barrel until the volume of the wooden barrel is three quarters of that of the wooden barrel; 2, forming a film called as vinegar mother on the surface of the liquid; and step 3, gradually taking out the obtained vinegar liquid and supplementing the vinegar liquid with grape wine or grape wine vinegar, wherein the whole process is carried out at a constant temperature of 20-40 DEG C.

The invention belongs to the technical field of plant extracts, and provides a stevia rebaudiana extract and a preparation method and application thereof.The preparation method comprises the following steps that 1, stevia rebaudiana leaves are taken, dried, smashed and sieved, and stevia rebaudiana powder is obtained; step 2, adding stevia rebaudiana powder into pure water, heating to 60-70 DEG C, stirring and extracting, and filtering to obtain filtrate I; step 3, adding a precipitant into the filtrate I, heating to 80-85 DEG C, stirring for 0.5-1 hour, adjusting the pH value, continuously stirring for 0.5-1 hour, keeping the temperature, standing for 4.0-4.5 hours, cooling to room temperature, and carrying out suction filtration to obtain filtrate II; step 4, adsorbing and eluting the filtrate II through macroporous adsorption resin to obtain an eluent; the preparation method of the stevia rebaudiana extract has the advantages that the operation is simple, the purity of the obtained stevia rebaudiana is high, and the stevia rebaudiana extract is suitable for large-scale production.

The application belongs to the field of agricultural biotechnology, and particularly relates to application of a low-phosphorus response gene SPX family of Stevia rebaudiana in improving glycoside content of Stevia rebaudiana. The application provides application of a low-phosphorus response gene SPX family of Stevia rebaudiana in improving glycoside content of Stevia rebaudiana, wherein the low-phosphorus response gene SPX family of Stevia rebaudiana comprises SrSPX2 a gene and SrSPX-RING4 a gene, a nucleotide sequence of the SrSPX2 gene is shown as SEQ ID NO: 1, and a nucleotide sequence of the SrSPX-RING4 gene is shown as SEQ ID NO: 2. The application determines the influence of low phosphorus on yield and quality of Stevia rebaudiana, and explores potential application value of a phosphorus regulation gene in improving quality of Stevia rebaudiana.

A Stevia extract made from leaves of the Stevia rebaudiana plant is described. The extract has desired levels of steviol glycosides and is useful in food, beverage, and other consumable products.

This invention provides a method for the simultaneous preparation of modified stevia leaves and crude glucosyl steviol glycosides, belonging to the field of food additives. The invention first involves disrupting the cell walls of stevia leaves using a plant cell wall-degrading enzyme, then directly adding a glycosyl donor using cyclodextrin glucosyltransferase as a catalyst in a one-pot glycosylation reaction. This allows for the simultaneous release and modification of steviol glycosides, achieving a final conversion rate of 68.57%. After the reaction, the mixture is filtered and freeze-dried, simultaneously obtaining modified stevia leaves with intact morphology and surface and pore interior loaded with modified glucosyl steviol glycosides, as well as crude glucosyl steviol glycoside powder. Sensory evaluation of the modified products showed that the modified stevia leaves exhibited significantly better sweetness, bitterness, and astringency than the unmodified leaves, and the crude glucosyl steviol glycosides had a significantly better taste than the extract from the unmodified stevia leaves. This invention features a short process flow, low cost, and achieves full utilization of stevia raw materials, demonstrating promising prospects for industrial application.

Stevia varieties with a high content of RebM, are disclosed Further provided are methods for producing Stevia plants having a high RebM content by negatively regulating certain genes selecting the resulting plants, and breeding with such plants to confer such desirable Reb M phenotypes to plant progeny.