66 results about "Stevia <genus>" patented technology

The invention discloses a genetically engineered bacteria used for producing stevia glycosyltransferase UGT76G1; a UGT76G1 coding gene is inserted between the restriction enzyme cutting sites EcoRI and XhoI of a PYes2 carrier, so as to construct a recombinant plasmid, and then the recombinant plasmid is introduced to expression host Saccharomyces cerevisiae YPH499 to obtain the engineered bacteria; and the coding gene of the UGT76G1 is GenBank, No. GenBank: AY345974.1, and the gene sequence of the coding gene is named as UGT (Udp Glucuronyl Transferase). The invention also discloses a construction method of the genetically engineered bacteria and the application of the bacteria to the production of rebaudioside A. According to the invention, under the condition that expensive UDPG (Uridine Diphosphate Glucose) is not added, cheap carbon source glucose is used as a substrate, the metabolic pathway of UDPG in the yeast is regulated, and then the rebaudioside A is produced from St glycosides through whole cell catalysis.

The invention relates to the tissue culture quick propagation technology of stevia rebaudiana seedlings, which comprises the following steps of: (1) selecting facilities; (2) selecting a female parent garden; (3) selecting a seed source; (4) preparing a culture medium; (5) obtaining explants; (6) performing subculture; and (7) performing rooting culture. By the tissue culture quick propagation technology, a large number of high-quality stevia rebaudiana seedlings can be cultured, the problem of short supply of stevia rebaudiana on the market is solved, the large-area planting of the stevia rebaudiana can be popularized, and national incomes are increased.

Highly purified Stevioside and Rebaudioside A were prepared from sweet glycoside extracts obtained from Stevia rebaudiana Bertoni leaves. The resulting sweeteners are suitable as non-calorie, non-cariogenic, non-bitter, non-lingering sweeteners, which may be advantageously applied in foods, beverages, and milk products.

The invention relates to the planting technology of stevia rebaudiana, which comprises the following steps of: selecting and retaining seeds, selecting a seedbed, preparing soil and furrowing, processing the seeds, sowing and managing in a seedling stage. By the technology, high-quality stevia rebaudiana seedlings can be cultivated, the yield of the stevia rebaudiana can be improved, and the problem of short supply of the stevia rebaudiana on the market can be solved, so the technology is favorable for popularizing the planting of the stevia rebaudiana in large area, and increasing national revenue.

The invention relates to a method for obtaining regenerated plants of stevia rebaudiana Bertoni, and belongs to the field of biotechnology. The method adopts anthers of stevia rebaudiana Bertoni as a material, and comprises the main steps of bud collection, low-temperature pretreatment and disinfection, callus induction culture, callus differentiation culture, adventitious bud rooting culture, and regenerated plant seedling and transplant. Through adoption of the method, a technical system of anther culture for stevia rebaudiana Bertoni is established, so that a stevia rebaudiana Bertoni plant tissue culture and plant regeneration system can be optimized, a certain substance research foundation can be laid for selective breeding of new varieties of stevia rebaudiana Bertoni, the breeding cycle of stevia rebaudiana Bertoni can be shortened, and new germplasm resources can be created for breeding of stevia rebaudiana Bertoni.

A simple extraction method for preparing an organic certifiable stevia extract with high sweetness and optionally with antioxidant properties is provided. The extraction method involves extraction of stevia leaves that have been dried and grinded with a hot solvent generally regarded as safe (GRAS), allowing the dissolution of the sweetening compounds naturally present therein. These sweetening compounds include steviosides and rebaudioside A, which are purified from the extracts by two or more successive chromatographic purifications steps. The sweetening compounds are then eluted with an appropriate elution solvent. The simplicity and organic certification of the method provide advantages over the methods currently employed in the stevia industry.

A Stevia extract made from leaves of the Stevia rebaudiana plant is described. The extract has desired levels of steviol glycosides and is useful in food, beverage, and other consumable products.

The invention discloses a method for cultivating autotetraploid plants of stevia rebaudiana. The method disclosed by the invention comprises the following steps of: taking adventitious buds of the stevia rebaudiana as a mutagenic material in an excised condition, and carrying out induced culture of tetraploids by adopting a method for combining a Colchicine solution with a tissue culture technology. By means of the method disclosed by the invention, higher inductivity can be obtained; a lot of tetraploid tissue culture seedlings can be obtained in a short time; compared with diploids, the obtained autotetraploid tissue culture seedling internodes of the stevia rebaudiana are short, dark in leaf colour and large and thick in leaf blade; and the advantages of polyploidy on morphology are initially embodied.

The invention provides a stevia rebaudiana variant plant which is 814011 Puxing No.3, a callus tissue is preserved by China General Microbiological Culture Collection Center (CGMCC) and has the preservation number of CGMCC No.9701. The invention also provides a high-RM-content stevioside product, wherein the RM / TSG ratio is greater than 0.08, and the RD / TSG ratio is greater than 0.08.

The invention discloses a dewatering and drying method for leaves of stevia rebaudiana Jiangtian No. 1. According to the method, stevia rebaudiana is harvested after drying of dew; an optimal harvesting period for the leaves of stevia rebaudiana is that 10% of stevia rebaudiana plants in a field are in a squaring stage (an early budding stage); harvested stem leaves should be protected from rain and piling; black leaves, yellow leaves and withered leaves should be removed at first before disleaving, then fresh and green leaves are sliped off by hand, and the rate of impurities like branches, buds, dust and silt of the plants in the leaves of stevia rebaudiana should be controlled within 10%; it should be guaranteed that disleaving is carried out as soon as the fresh leaves are harvested, the leaves are dried in the sun once after disleaving is carried out and the leaves are dried within one day so as to ensure dry leaf quality. Drying standards for the leaves of stevia rebaudiana are that water content in the leaves is less than 10% and are frangible when touched with hand. The dried leaves of stevia rebaudiana have to be put into a moistureproof food packaging bag or polyethylene plastic bag in time, and the mouth of the bag has to be fastened and closed so as to prevent the leaves of stevia rebaudiana from mildew because of moisture absorption under natural conditions. The method provided by the invention is more suitable for dewatering and drying of the leaves of stevia rebaudiana Jiangtian No. 1.

The invention provides a patrima villosa beverage and a preparation method thereof. The beverage comprises patrima villosa, stevia rebaudiana and water serving as raw materials. The method comprises the following steps of: mixing the cleaned patrima villosa and the cleaned stevia rebaudiana by adding water, heating, boiling, keeping the boiling state for 8 to 15 minutes, filtering the liquid, sterilizing, cooling, filling, and thus obtaining a finished product. The beverage is convenient to eat, has sweet mouthfeel, and has good health-care effect on the human body; and the preparation method is simple, convenient, easy in operation and suitable for large-scale popularization.

The invention provides a high-quality tomato cultivation method, which comprises the following steps: planting stevia rebaudiana before tomato cultivation, turning over and returning to the field in situ, and planting tomatoes after stevia rebaudiana straws are moderately decomposed. According to the cultivation method of the high-quality tomatoes, the stevia rebaudiana is sown before the tomatoes are planted, and the stevia rebaudiana is turned, pressed and returned to the field in situ in the squaring stage, so that the normal yield of the tomatoes can be ensured on the premise of not controlling water in the whole growth period of the tomatoes, the problem that the tomatoes are low in nutritional quality and flavor quality can be solved, and the yield of the tomatoes is increased under the normal yield level. And the soluble solid content of the tomatoes is increased from 4.3%-4.7% to 6.8%-8.1%.

The invention discloses a hybrid seed production method for stevia rebaudiana. The method comprises the steps that firstly, cutting propagation is conducted on parent plants of the stevia rebaudiana, colonies which are consistent in genotype and reproduction characteristic are formed, the colonies serve as parent strains of hybrid seed production, two of the parent strains are planted in the same field in a lined and spaced mode, natural hybridization between the parent strains is conducted, and stevia rebaudiana hybrid seeds can be obtained. By means of the method, defects that judicious hybrid cannot be conducted on seedling colonies of stevia rebaudiana seeds, the colony segregation is large, and characters are not stable are overcome, and the excellent stevia rebaudiana hybrid seed combination which is comparatively stable in character can be provided.

Stevia compositions are prepared from steviol glycosides of Stevia Rebaudiana Bertoni. The compositions are able to provide a superior taste profile and can be used as sweetness enhancers, flavor enhancers and sweeteners in foods, beverages, cosmetics and pharmaceuticals.

A Stevia extract comprises one or more Stevia-derived non-steviol glycoside substances and / or one or more non-volatile non-steviol glycoside substances. The Stevia extract may be used as a sweetener or a flavorant in an orally consumable product.

Stevia extracts with selected major steviol glycosides (Reb A, stevioside, Reb D, Reb C) and minor steviol glycosides and glycosylated diterpene derivative plant molecules, derived from Stevia rebaudiana plant are found to improve the perception of flavor and taste perception, which includes the sweet, savory and salty perception in a wide range of food and beverage applications.

Stevia compositions are prepared from steviol glycosides of Stevia rebaudiana Bertoni. The compositions are able to provide a superior taste profile and can be used as sweetness enhancers, flavor enhancers and sweeteners in foods, beverages, cosmetics and pharmaceuticals.

The invention discloses a matched cultivation method of stevia rebaudiana triploid hybrids. The method comprises steps as follows: diploid materials of stevia rebaudiana are subjected to chromosome doubling, individual stevia rebaudiana plant materials of tetraploids and diploids to be subjected to matching hybridization are subjected to cutting propagation respectively, a large quantity of seed production parent strains of tetraploid and diploid stevia rebaudiana with consistent genetypes and reproductive traits are obtained respectively, the two seed production parent strains are planted in rows in the same field alternately for hybrid seed production, and due to the self-incompatible characteristic of the stevia rebaudiana, the stevia rebaudiana triploid hybrids are obtained after natural hybridization. According to the huge plant triploid organs, the leaf yield of the stevia rebaudiana can be remarkably increased with the adoption of the measure.

The invention discloses application of stevioside in promotion of growth of chlorella. With respect to the prior art, the invention provides application of stevioside or stevia rebaudiana leaching liquor in promotion of the growth of the chlorella and improvement of culture efficiency. Under certain conditions, a right amount of stevioside or stevia rebaudiana leaching liquor is added, the growth rate of the chlorella can be obviously increased, and the quantity of the chlorella in a culture solution is increased, so that the culture efficiency and yield of the chlorella are improved, and the stevioside or stevia rebaudiana leaching liquor is applicable to further popularization and application.

A method for making a granulated Stevia sweetener is described. The resulting sweetener has a desirably high solubility level.

The invention discloses a mating method for stevia rebaudiana hybrid seed production. The mating method includes the steps that the parent single plant of stevia rebaudiana is subjected to tissue culture and propagation, parent plant systems which are consistent in genotype and reproduction feature are formed, the two different parent plant systems are planted alternately in rows in the same field to conduct mutual natural hybridization, and stevia rebaudiana hybrid seeds are obtained. The mating method overcomes the defects that stevia rebaudiana seed seedling groups cannot conduct directional hybridization, group separation is large and characters are instable, and good stevia rebaudiana hybrid seed combinations which are relatively stable in character can be provided.

The invention provides a stevia rebaudiana variant plant which is 817096 Puxing No.5, a callus tissue is preserved by China General Microbiological Culture Collection Center (CGMCC) and has the preservation number of CGMCC No.9703. The invention also provides a high-RD-content stevioside product, wherein the RD / TSG ratio is greater than 0.28.

The invention discloses a secondary utilization method of a rooting medium for stevia rebaudiana. After tissue cultured rooted seedlings of stevia rebaudiana are cultured, the rooted seedlings are pulled out of a culture bottle, and single-node stems of stevia rebaudiana are reconnected to an original medium to be subjected to secondary rooting culture, so that residual nutrient substances in the medium can be fully used, and a crop of rooted seedlings can be re-obtained. The measure can save the tissue culture cost of stevia rebaudiana, thereby improving the economic benefits.

The present disclosure relates generally to terpene glycosides, such as certain such compounds extracted from Stevia rebaudiana Bertoni, Rubus suavissimus, or Siraitia grosvenorii. The disclosure alsoprovides for the use of such compounds as food ingredients, flavors, and sweeteners, and related methods. The disclosure also provides ingestible compositions comprising such compounds, as well as processes for extracting such compounds selectively from certain plant sources.

The invention belongs to the field of genetic engineering, and specifically relates to a method for producing chlorogenic acid and dicaffeoylquinic acid by using Agrobacterium rhizogenes to genetically transform stevia rebaudiana to establish a hairy root culture system. The content includes the preparation of stevia rebaudiana explants, the activation culture of Agrobacterium rhizogenes, the suspension culture and the production of chlorogenic acid and dicaffeoylquinic acid. The detection by PCR proved that the hairy root of Stevia rebaudiana was produced by transformation, and the detection by HPLC showed that the hairy root of Stevia rebaudiana could produce chlorogenic acid and dicaffeoylquinic acid. Since the hairy roots used have the characteristics of rapid growth on the hormone-free medium, the method has the advantages of simple operation, low cost, and not being restricted by natural conditions such as climate and land. It provides a stable and sustainable new drug source and food functional factor for the production of chlorogenic acid and dicaffeoylquinic acid by using hairy root culture. The large-scale production of acid provides a reliable source and material basis.

The invention discloses a natural stevia rebaudiana functional healthcare drink which is made of the following raw materials in parts by weight: 20-30 parts of stevia rebaudiana, 10-15 parts of carrot, 10-15 parts of dates, 10-15 parts of lycium barbarum, 3-5 parts of radix astragali and 1-3 parts of mint. The invention further discloses a preparation method of the natural stevia rebaudiana functional healthcare beverage. The stevia rebaudiana and the lycium barbarum of the drink both have hypoglycemic effects, and the radix astragali, the carrot, the dates and the like in the drink have effects of improving human immunity, so that the drink has a good healthcare effect and has an anti-cancer effect when taken for a long time.

A Stevia rebaudiana plant is characterized by Rebaudioside A (RA) content of 6-20% dry weight and total steviol glycosides content of 15-28% dry weight in the leaf. The plant is developed using selective breeding technologies and identified by RAPD gene analysis.

The invention relates to the technical field of stevia rebaudiana planting, in particular to a planting device for soilless culture of stevia rebaudiana. According to the device, through joint cooperation of a mounted feeding hopper, a switching mechanism, a box body and a stirring assembly, a rotating handle is rotated to enable the stirring assembly to uniformly stir an added nutrient solution, meanwhile, a first belt wheel and a second belt wheel are driven to rotate, a liquid pump is started to pump the nutrient solution and convey the nutrient solution into a cultivation groove, an adjustable illumination structure is adjusted up and down, and an illumination lamp is driven to move up and down to illuminate stevia rebaudiana plants, so that photosynthesis of stevia rebaudiana is facilitated. A control motor drives an adjustable cultivation assembly to adjust the height, so that the roots of the stevia rebaudiana plants are in contact with the nutrient solution conveniently, and the upper parts of the plants are prevented from being soaked in the nutrient solution. When the nutrient solution needs to be replaced, a corresponding valve is switched on. The nutrient solution is discharged into a liquid discharging cavity through the switching mechanism, a liquid discharging valve is switched on to discharge the nutrient solution, cultivation personnel can replace the nutrient solution rapidly and conveniently, and growth of stevia rebaudiana plants is facilitated.