9 results about "Stevia <genus>" patented technology

A stevia cultivar containing a high Rebaudioside M content, designated ‘18136109’, is disclosed. The invention relates to the plant parts of stevia cultivar ‘18136109’, to the plants of stevia ‘18136109’ and to methods for producing a stevia plant produced by crossing the cultivar ‘18136109’ with itself or another stevia variety, including methods using marker assisted breeding. The invention further relates to hybrid stevia seeds and plants produced by crossing the cultivar ‘18136109’ with another stevia cultivar. Twelve highly polymorphic SNPs loci and the corresponding genomic sequences used to identify plant variety ‘18136109’-derived plant materials are also disclosed.

The application discloses a method for improving the germination rate of stevia seed, and specifically comprises the following steps: selecting full and healthy stevia seed with consistent size for standby; disinfecting the stevia seed with 10% sodium hypochlorite solution for 5-15 minutes, then rinsing with distilled water for multiple times and filtering dry; preparing different concentrations of salicylic acid to pretreat the stevia seed; taking equal amounts of salicylic acid with different concentrations into at least three portions, and adding equal amounts of the disinfected stevia seed into each portion for seed soaking; after the seed soaking, rinsing with distilled water for 3-5 times, and performing seed germination test by adopting the culture dish filter paper method; starting observation and recording from the day when the stevia seed is placed on the bed; and comparing the germination of the stevia seed. The method for improving the germination rate of stevia seed has the advantages of convenience, high efficiency, non-toxicity, non-residue, low cost, high economic benefit, and wide application prospect in modern efficient agricultural production.

The invention relates to the technical field of pet hair repair, in particular to a hair repair composition for pet cats as well as a preparation method and application thereof, and the hair repair composition comprises stevia rebaudiana extract and inositol. The stevia rebaudiana extract and the inositol are mixed according to the mass ratio of 1: (5-15). The preparation method comprises the following steps: (1) respectively weighing the stevia rebaudiana extract and the inositol according to the mass ratio, adding purified water, and stirring and dissolving at 40-60 DEG C to obtain a mixed solution for later use; and (2) drying the mixed solution in the step (1) to obtain powder, namely the hair repairing composition. The hair repairing composition provided by the invention can be used for remarkably improving the hair glossiness, smoothness and elasticity of pet cats.

The application discloses a preparation method of a stevia extract, and belongs to the technical field of plant extract preparation; the preparation method comprises the following steps: S1, crushing stevia raw materials, then soaking the stevia raw materials in water, adding a composite enzyme to perform enzymolysis, and preparing an enzymolysis solution; S2, performing flocculation treatment on the enzymolysis solution; the flocculating agent is a composite flocculating agent, and a crude extract solution is obtained; S3, performing gradient elution on the crude extract solution after adsorption by using a macroporous resin, and collecting eluate of a specific section; and S4, performing elution after adsorption of the eluate by using an aluminum oxide column, concentrating the eluate, and crystallizing; the preparation method only uses water and ethanol as solvents in the whole process, does not use toxic organic reagents, is simple to operate, and is very suitable for industrial mass production.

The utility model discloses cleaning and impurity filtering equipment for stevia rebaudiana extraction, which belongs to the technical field of cleaning equipment, and comprises a base, one end of the base is fixedly connected with a cleaning box, the bottom end of the cleaning box is provided with a water outlet, the other end of the base is fixedly connected with a support frame, and the top end of the support frame is welded with a movable frame. An electric push rod is bolted to the outer side wall of the moving frame, a push rod part of the electric push rod penetrates through the moving frame and is provided with a moving seat, a stirring assembly is arranged at the top end of the moving seat, a water storage tank is fixedly connected to the upper surface of the base, and a flushing mechanism is arranged at one end of the water storage tank; a filter plate for filtering impurities in water is arranged in an inner cavity of the water storage tank, so that the cleaning speed of the stevia rebaudiana is increased, the influence on the extraction efficiency of the stevia rebaudiana is reduced, the cleaning water of the stevia rebaudiana can be recycled, and the waste of resources is reduced.

The invention belongs to the technical field of agricultural biology, and particularly relates to application of a stevia rebaudiana low-phosphorus response gene PHR family in improving the content of glycoside in stevia rebaudiana. The invention provides application of a stevia rebaudiana low-phosphorus response gene PHR family in improving the content of glycoside in stevia rebaudiana, the stevia rebaudiana low-phosphorus response gene PHR family comprises a SrPHR1 gene and a SrPHR13 gene, and the relative content of rebaudioside C, steviobioside, rebaudioside D and total glycoside is improved by overexpressing the SrPHR1 gene; the relative content of stevioside and rebaudioside A is reduced through overexpression of the SrPHR1 gene; the relative contents of rebaudioside A, rebaudioside C and total glycosides are increased by overexpressing the SrPHR13 gene; the relative content of stevioside and rebaudioside D is reduced through overexpression of the SrPHR13 gene. The invention clarifies the influence of low phosphorus on the yield and quality of stevia rebaudiana, and preliminarily explores the application value of the phosphorus regulatory gene in improving the quality of stevia rebaudiana.

The application belongs to the field of agricultural biotechnology, and particularly relates to application of a low-phosphorus response gene SPX family of Stevia rebaudiana in improving glycoside content of Stevia rebaudiana. The application provides application of a low-phosphorus response gene SPX family of Stevia rebaudiana in improving glycoside content of Stevia rebaudiana, wherein the low-phosphorus response gene SPX family of Stevia rebaudiana comprises SrSPX2 a gene and SrSPX-RING4 a gene, a nucleotide sequence of the SrSPX2 gene is shown as SEQ ID NO: 1, and a nucleotide sequence of the SrSPX-RING4 gene is shown as SEQ ID NO: 2. The application determines the influence of low phosphorus on yield and quality of Stevia rebaudiana, and explores potential application value of a phosphorus regulation gene in improving quality of Stevia rebaudiana.

This invention provides a method for the simultaneous preparation of modified stevia leaves and crude glucosyl steviol glycosides, belonging to the field of food additives. The invention first involves disrupting the cell walls of stevia leaves using a plant cell wall-degrading enzyme, then directly adding a glycosyl donor using cyclodextrin glucosyltransferase as a catalyst in a one-pot glycosylation reaction. This allows for the simultaneous release and modification of steviol glycosides, achieving a final conversion rate of 68.57%. After the reaction, the mixture is filtered and freeze-dried, simultaneously obtaining modified stevia leaves with intact morphology and surface and pore interior loaded with modified glucosyl steviol glycosides, as well as crude glucosyl steviol glycoside powder. Sensory evaluation of the modified products showed that the modified stevia leaves exhibited significantly better sweetness, bitterness, and astringency than the unmodified leaves, and the crude glucosyl steviol glycosides had a significantly better taste than the extract from the unmodified stevia leaves. This invention features a short process flow, low cost, and achieves full utilization of stevia raw materials, demonstrating promising prospects for industrial application.

Stevia varieties with a high content of RebM, are disclosed Further provided are methods for producing Stevia plants having a high RebM content by negatively regulating certain genes selecting the resulting plants, and breeding with such plants to confer such desirable Reb M phenotypes to plant progeny.