64 results about "Stevia <genus>" patented technology

The invention discloses a genetically engineered bacteria used for producing stevia glycosyltransferase UGT76G1; a UGT76G1 coding gene is inserted between the restriction enzyme cutting sites EcoRI and XhoI of a PYes2 carrier, so as to construct a recombinant plasmid, and then the recombinant plasmid is introduced to expression host Saccharomyces cerevisiae YPH499 to obtain the engineered bacteria; and the coding gene of the UGT76G1 is GenBank, No. GenBank: AY345974.1, and the gene sequence of the coding gene is named as UGT (Udp Glucuronyl Transferase). The invention also discloses a construction method of the genetically engineered bacteria and the application of the bacteria to the production of rebaudioside A. According to the invention, under the condition that expensive UDPG (Uridine Diphosphate Glucose) is not added, cheap carbon source glucose is used as a substrate, the metabolic pathway of UDPG in the yeast is regulated, and then the rebaudioside A is produced from St glycosides through whole cell catalysis.

The invention relates to the tissue culture quick propagation technology of stevia rebaudiana seedlings, which comprises the following steps of: (1) selecting facilities; (2) selecting a female parent garden; (3) selecting a seed source; (4) preparing a culture medium; (5) obtaining explants; (6) performing subculture; and (7) performing rooting culture. By the tissue culture quick propagation technology, a large number of high-quality stevia rebaudiana seedlings can be cultured, the problem of short supply of stevia rebaudiana on the market is solved, the large-area planting of the stevia rebaudiana can be popularized, and national incomes are increased.

The invention relates to the planting technology of stevia rebaudiana, which comprises the following steps of: selecting and retaining seeds, selecting a seedbed, preparing soil and furrowing, processing the seeds, sowing and managing in a seedling stage. By the technology, high-quality stevia rebaudiana seedlings can be cultivated, the yield of the stevia rebaudiana can be improved, and the problem of short supply of the stevia rebaudiana on the market can be solved, so the technology is favorable for popularizing the planting of the stevia rebaudiana in large area, and increasing national revenue.

The invention relates to a method for obtaining regenerated plants of stevia rebaudiana Bertoni, and belongs to the field of biotechnology. The method adopts anthers of stevia rebaudiana Bertoni as a material, and comprises the main steps of bud collection, low-temperature pretreatment and disinfection, callus induction culture, callus differentiation culture, adventitious bud rooting culture, and regenerated plant seedling and transplant. Through adoption of the method, a technical system of anther culture for stevia rebaudiana Bertoni is established, so that a stevia rebaudiana Bertoni plant tissue culture and plant regeneration system can be optimized, a certain substance research foundation can be laid for selective breeding of new varieties of stevia rebaudiana Bertoni, the breeding cycle of stevia rebaudiana Bertoni can be shortened, and new germplasm resources can be created for breeding of stevia rebaudiana Bertoni.

A simple extraction method for preparing an organic certifiable stevia extract with high sweetness and optionally with antioxidant properties is provided. The extraction method involves extraction of stevia leaves that have been dried and grinded with a hot solvent generally regarded as safe (GRAS), allowing the dissolution of the sweetening compounds naturally present therein. These sweetening compounds include steviosides and rebaudioside A, which are purified from the extracts by two or more successive chromatographic purifications steps. The sweetening compounds are then eluted with an appropriate elution solvent. The simplicity and organic certification of the method provide advantages over the methods currently employed in the stevia industry.

The invention discloses a method for cultivating autotetraploid plants of stevia rebaudiana. The method disclosed by the invention comprises the following steps of: taking adventitious buds of the stevia rebaudiana as a mutagenic material in an excised condition, and carrying out induced culture of tetraploids by adopting a method for combining a Colchicine solution with a tissue culture technology. By means of the method disclosed by the invention, higher inductivity can be obtained; a lot of tetraploid tissue culture seedlings can be obtained in a short time; compared with diploids, the obtained autotetraploid tissue culture seedling internodes of the stevia rebaudiana are short, dark in leaf colour and large and thick in leaf blade; and the advantages of polyploidy on morphology are initially embodied.

The invention provides a stevia rebaudiana variant plant which is 814011 Puxing No.3, a callus tissue is preserved by China General Microbiological Culture Collection Center (CGMCC) and has the preservation number of CGMCC No.9701. The invention also provides a high-RM-content stevioside product, wherein the RM/TSG ratio is greater than 0.08, and the RD/TSG ratio is greater than 0.08.

The invention provides a high-quality tomato cultivation method, which comprises the following steps: planting stevia rebaudiana before tomato cultivation, turning over and returning to the field in situ, and planting tomatoes after stevia rebaudiana straws are moderately decomposed. According to the cultivation method of the high-quality tomatoes, the stevia rebaudiana is sown before the tomatoes are planted, and the stevia rebaudiana is turned, pressed and returned to the field in situ in the squaring stage, so that the normal yield of the tomatoes can be ensured on the premise of not controlling water in the whole growth period of the tomatoes, the problem that the tomatoes are low in nutritional quality and flavor quality can be solved, and the yield of the tomatoes is increased under the normal yield level. And the soluble solid content of the tomatoes is increased from 4.3%-4.7% to 6.8%-8.1%.

The invention discloses a hybrid seed production method for stevia rebaudiana. The method comprises the steps that firstly, cutting propagation is conducted on parent plants of the stevia rebaudiana, colonies which are consistent in genotype and reproduction characteristic are formed, the colonies serve as parent strains of hybrid seed production, two of the parent strains are planted in the same field in a lined and spaced mode, natural hybridization between the parent strains is conducted, and stevia rebaudiana hybrid seeds can be obtained. By means of the method, defects that judicious hybrid cannot be conducted on seedling colonies of stevia rebaudiana seeds, the colony segregation is large, and characters are not stable are overcome, and the excellent stevia rebaudiana hybrid seed combination which is comparatively stable in character can be provided.

A Stevia extract comprises one or more Stevia-derived non-steviol glycoside substances and/or one or more non-volatile non-steviol glycoside substances. The Stevia extract may be used as a sweetener or a flavorant in an orally consumable product.

The invention discloses a matched cultivation method of stevia rebaudiana triploid hybrids. The method comprises steps as follows: diploid materials of stevia rebaudiana are subjected to chromosome doubling, individual stevia rebaudiana plant materials of tetraploids and diploids to be subjected to matching hybridization are subjected to cutting propagation respectively, a large quantity of seed production parent strains of tetraploid and diploid stevia rebaudiana with consistent genetypes and reproductive traits are obtained respectively, the two seed production parent strains are planted in rows in the same field alternately for hybrid seed production, and due to the self-incompatible characteristic of the stevia rebaudiana, the stevia rebaudiana triploid hybrids are obtained after natural hybridization. According to the huge plant triploid organs, the leaf yield of the stevia rebaudiana can be remarkably increased with the adoption of the measure.

The invention discloses a mating method for stevia rebaudiana hybrid seed production. The mating method includes the steps that the parent single plant of stevia rebaudiana is subjected to tissue culture and propagation, parent plant systems which are consistent in genotype and reproduction feature are formed, the two different parent plant systems are planted alternately in rows in the same field to conduct mutual natural hybridization, and stevia rebaudiana hybrid seeds are obtained. The mating method overcomes the defects that stevia rebaudiana seed seedling groups cannot conduct directional hybridization, group separation is large and characters are instable, and good stevia rebaudiana hybrid seed combinations which are relatively stable in character can be provided.

The invention discloses a secondary utilization method of a rooting medium for stevia rebaudiana. After tissue cultured rooted seedlings of stevia rebaudiana are cultured, the rooted seedlings are pulled out of a culture bottle, and single-node stems of stevia rebaudiana are reconnected to an original medium to be subjected to secondary rooting culture, so that residual nutrient substances in the medium can be fully used, and a crop of rooted seedlings can be re-obtained. The measure can save the tissue culture cost of stevia rebaudiana, thereby improving the economic benefits.

The invention discloses a natural stevia rebaudiana functional healthcare drink which is made of the following raw materials in parts by weight: 20-30 parts of stevia rebaudiana, 10-15 parts of carrot, 10-15 parts of dates, 10-15 parts of lycium barbarum, 3-5 parts of radix astragali and 1-3 parts of mint. The invention further discloses a preparation method of the natural stevia rebaudiana functional healthcare beverage. The stevia rebaudiana and the lycium barbarum of the drink both have hypoglycemic effects, and the radix astragali, the carrot, the dates and the like in the drink have effects of improving human immunity, so that the drink has a good healthcare effect and has an anti-cancer effect when taken for a long time.

The invention provides a rapid grinding device for sugar making of stevia rebaudiana, and relates to the technical field of stevia rebaudiana processing.The rapid grinding device comprises a main box,a shearing mechanism and a grinding mechanism, wherein a treatment box is fixedly connected to the top of the main box, a mounting plate is fixedly connected to the output end of an air cylinder, a cutter is fixedly connected to the left side of the mounting plate, an air conveying pipe is connected to the output end of a fan, a filter screen is installed at the bottom of the treatment box, one end of a movable plate is hinged to a connecting base, a telescopic rod is obliquely arranged on the side wall of an inner cavity of the main box, and the output end of the telescopic rod abuts againstthe bottom of the movable plate. The air cylinder drives the cutter to move back and forth to cut up dry stevia rebaudiana leaves driven by wind energy, so that the dry stevia rebaudiana leaves can be refined into the size capable of passing through the filter screen, then the movable plate is driven by the telescopic rod to impact the filter screen, the dry stevia rebaudiana leaves can fall downand enter the grinding box along the movable plate, the subsequent grinding difficulty is reduced, and the grinding effect is improved.

The present invention provides a stevia plant having a low ability to form flower buds as compared with the wild type stevia species. The present invention also provides a method of producing such a stevia plant having a low ability to form flower buds, and an extract or a steviol glycoside purified product obtainable from such a plant.