206 results about "Steviolmonoside" patented technology

The invention belongs to the technical field of biosynthesis, and particularly relates to an engineering bacterium for preparing stevioside through de novo fermentation as well as a preparation method and application of the engineering bacterium. According to the invention, mevalonate kinase in the MVA pathway is mutated and optimized. The method comprises the following steps: carrying out point mutation on mevalonate kinase MvK of a wild type source, constructing a stevioside synthesis route in an escherichia coli host, and comparing the influence of MvK mutation on the yield of stevioside, so as to determine a Q160L mutant, and applying the Q160L mutant to the stevioside production route to realize efficient production of stevioside.

The invention discloses a method for preparing low-grafted glucosyl stevioside at high temperature and high pressure, which comprises the following steps: taking stevioside as a receptor, beta-cyclodextrin as a donor and CGT enzyme as a catalyst for reaction to obtain reaction liquid containing glucosyl stevioside; putting the reaction liquid containing the glucosyl stevioside into a high-pressure reaction kettle, and adding a catalyst to carry out high-temperature and high-pressure cracking reaction; after the high-temperature and high-pressure cracking reaction is finished, cooling a cracking solution to room temperature, filtering, concentrating for multiple times by adopting a polymeric membrane, and collecting a trapped fluid; and carrying out spray drying on the trapped fluid to obtain the low-grafting glucosyl stevioside. According to the method, firstly, glucosyl stevioside is prepared by adopting an enzyme modification method, then splitting decomposition is performed under certain conditions, and then a specific purification process is performed, so that the prepared product is high in stability, the proportion of low-grafted glucosyl stevioside in the product is high, and the product tastes good.

The invention belongs to the technical field of biosynthesis, and particularly relates to a method for regulating and controlling stevioside production through a UDPG synthesis route. According to the invention, a high-efficiency stevioside biosynthesis system is constructed through systematic engineering modification: in the aspect of chassis cell optimization, transcription factors (AtWRKY18, AtWRKY40 and AtMYC2) for regulating and controlling MEP pathway and key genes (dxs and / or dxr) of the MEP pathway are over-expressed, so that supply of terpene precursors is enhanced; and a heterologous MVA pathway (over-expression T7-atoB-mvaS-mvaE-mvK / mvKmut-pmK-mvaD-idi) with a modular design is introduced, so that sufficient supply of the terpenoid precursor is realized. Meanwhile, the intracellular concentration of the UDPG is increased by systematically enhancing a UDPG synthesis pathway (knocking out ugd, glgc, ushA, agp, deoA, tdk, udp, or thrA, and overexpressing galU, pgm, pyrE, pyrH, ugpA, or prs), so that the production capacity of the stevioside is remarkably improved.

The invention belongs to the technical field of biosynthesis, and particularly relates to a method for regulating and controlling stevioside production by improving the utilization rate of a carbon source. According to the invention, a high-efficiency stevioside biosynthesis system is constructed through systematic engineering modification: in the aspect of chassis cell optimization, transcription factors (AtWRKY18, AtWRKY40 and AtMYC2) for regulating and controlling MEP pathway and key genes (dxs and / or dxr) of the MEP pathway are over-expressed, so that supply of terpene precursors is enhanced; and a heterologous MVA pathway (over-expression T7-atoB-mvaS-mvaE-mvK / mvKmut-pmK-mvaD-idi) with a modular design is introduced, so that sufficient supply of the terpenoid precursor is realized. Meanwhile, by knocking out related genes and bypass metabolism genes (mgsA, iclR, menA or adhE) synthesized by organic acid, a glucose transport and utilization system (fnr, fadR, ppc, zwf or pgi is knocked out, and ACS, fbaA, tpiA, glf, galP or ptsG is overexpressed, so that the utilization rate of a carbon source is further improved, and the production capacity of stevioside is remarkably improved.

The invention relates to a stevioside composition, which comprises at least one stevioside and at least one of siamenoside I, mogroside V, mogroside III-E and mogroside II-E, and is characterized in that the stevioside composition comprises at least one stevioside and at least one of siamenoside I, mogroside V, mogroside III-E and mogroside II-E; wherein the stevioside has a higher solubility in the composition than in an equivalent composition lacking siamenoside I, mogroside V, mogroside III-E, and mogroside II-E. The invention also relates to a method for preparing the stevioside composition. The composition may be an aqueous solution.

The invention relates to the technical field of beverages, and particularly discloses a high-protein beverage containing psicose and a preparation method of the high-protein beverage. The high-protein beverage containing psicose is prepared from the following raw materials in percentage by mass: 13 to 17 percent of supplementary protein, 4 to 5 percent of an emulsifier, 3.10 to 3.14 percent of a flavoring agent, 0.02 to 0.06 percent of a thickening agent, 0.005 to 0.015 percent of a stabilizer, 0.008 to 0.01 percent of a coloring agent, 0.04 to 0.08 percent of an enzyme preparation and the balance of water, the flavoring agent is prepared from D-psicose, erythritol, sucralose and stevioside. The D-psicose in the flavoring agent is extremely low in calorie, does not cause increase of blood sugar and insulin, and has a unique taste, and the erythritol, the sucralose and the stevioside are matched, so that the taste of sucrose is reduced on the premise of low calorie, and the problem of bitter taste of a sugar substitute beverage is solved, so that the flavor of the protein beverage is improved.

The invention relates to the technical field of functional beverages, in particular to a composite polypeptide solid beverage with a uric acid reducing function and a preparation method of the composite polypeptide solid beverage. The composite polypeptide solid beverage with the uric acid reducing function is prepared from the following raw materials in percentage by weight: 15-25% of plukenetia volubilis extract, 30-40% of lysimachia christinae hance-plantain herb composite extract, 8-12% of oyster peptide powder, 5-8% of earthworm protein peptide freeze-dried powder and 3-5% of a flavoring agent. Wherein the ratio of a lysimachia christinae hance extract to a plantain herb extract in the lysimachia christinae hance-plantain herb composite extract is 3: The flavoring agent is erythritol and / or stevioside, xanthine oxidase is inhibited by using flavonoid substances, GLUT9 expression is synergistically down-regulated by using bispyribaloside, PRPP synthetase activity is regulated by using zinc-rich peptide, uric acid micro-crystals are dissolved by using earthworm protein peptide, a multi-target action network of flavonoid-glycoside-bioactive peptide is achieved, and the effect of improving the bioavailability of the flavonoid-glycoside-bioactive peptide is achieved. And triple mechanisms of excretion promotion, generation inhibition and kidney protection are realized.

The invention provides a protein component composition with special medical application as well as a preparation method and application thereof, and relates to the field of formula food with special medical application. Wherein the protein component composition comprises protein and a sweetening agent; the protein is one or more of hydrolyzed whey protein powder, hydrolyzed casein and albumin peptide. The sweetening agent is one or more of white granulated sugar, glucose syrup, malt syrup, high fructose corn syrup, erythritol, xylitol, maltitol and stevioside. A plurality of components such as a stabilizer, mineral substances and multivitamins can also be added into the protein component composition. The finally obtained composition can be applied to preparation of formula food for special medical purposes, and the content of total protein, hemoglobin, albumin or transferrin in blood of patients suffering from malnutrition or insufficient protein intake is increased.

Disclosed herein are formulations (including sweet proteins and rare sugars) for improving the sweetness of low glycemic index rare sugars without altering the texture and flavor of the rare sugars and methods for improving the sweetness of rare sugars. More specifically, the present invention discloses methods for improving the sweetness of rare sugars without altering the sweetness profile, texture and flavor of the primary sugars. The invention also discloses the addition of Brazitame protein with liquid rare sugars at different temperatures during the synthesis of the rare sugars, before crystallization or before or after caramelization of the rare sugars, to increase sweetness. The Brazilian sweet protein increases the sweetness of rare sugars, but is not limited to D-psicose, D-tagatose, D-allose, isomaltulose, trehalose, sorbose, pinose, leucobiose and the like. The invention also provides for the evaluation of non-nutritional sweeteners (such as aspartame, acesulfame, sodium cyclamate, neotame, saccharin, sucralose, stevioside, etc.), as each has a limitation of taste quality if used with rare sugars. However, the use of Brazilian sweet protein does not alter the flavor and texture of rare sugars, and the taste profile matches sucrose.

The invention relates to the technical field of biological catalysis engineering, in particular to a method for improving the solubility of stevioside Reb M and Reb D through enzyme catalysis modification, which comprises the following steps: dissolving Reb M and Reb D with the purity of more than or equal to 90% in a buffer solution with the pH value of 4.0-8.0, adding a non-starch glycosyl donor and composite glycosyl transferase according to the enzyme-substrate mass ratio of 0.05-0.5, and optimizing the enzyme addition sequence to realize specific site modification; reacting for 6-24 hours under the conditions that the pH is 5.5-6.0 and the temperature is 60-85 DEG C, and controlling the proportion of DP2-3 to be 60-80%; hPLC sampling is carried out every 4-6 hours, and the unreacted substrate is less than or equal to 20%; the solubility of the product is greater than or equal to 25g / L, and the sweet taste of the product is pure. According to the method, the solubility of Reb M / Reb D is improved, the structure of a directional glycosylation controlled product is realized, and pure sweetness is reserved; the reaction is mild and controllable, enzyme inactivation is simple and free of residues, industrialization is easy, and stevioside application upgrading is promoted.

The invention relates to chicken skin fruity qi and blood tonifying soft sweets and a making method thereof, and belongs to the technical field of health food. The invention provides chicken skin fruity qi and blood tonifying soft sweets. The chicken skin fruity qi and blood tonifying soft sweets are prepared from the following components in parts by mass: 37 to 99 parts of chicken skin juice, 15 to 30 parts of red dates, 10 to 25 parts of radix astragali seu hedysari, 5 to 15 parts of radix angelicae sinensis, 1 to 8 parts of radix ophiopogonis, 65 to 83 parts of gelatin, 15 to 25 parts of agar, 130 to 160 parts of xylitol, 0.1 to 0.6 part of stevioside, 0.1 to 0.6 part of mogroside, 1 to 8 parts of citric acid and 150 to 240 parts of water. The chicken skin fruity qi and blood tonifying soft sweets have the effects of tonifying qi and blood, resisting oxidation, reducing blood sugar, preventing decayed teeth, enhancing the immune regulation ability and the like, the variety of soft sweets is enriched, the market diversification requirements are met, high-quality development of special agricultural products can be promoted, the supply chain of the agricultural industry chain is prolonged, and the market prospect is wide. The method has profound significance on development of special agricultural products and economy.

The utility model discloses cleaning equipment for stevioside production, and relates to the technical field of stevioside preparation. The conveying mechanism is arranged in the cleaning frame and used for conveying stevioside raw materials, the conveying mechanism comprises a driving part and a conveying belt, the driving part is arranged in the cleaning frame, the conveying belt is arranged on the driving part, and a plurality of conveying plates are fixedly connected to the conveying belt at equal intervals; and the cleaning mechanism is arranged in the cleaning frame and is used for cleaning the stevioside raw material. According to the cleaning equipment for stevioside production, the conveying mechanism is matched with the cleaning mechanism for use, raw materials of stevioside are dispersed, the effect of the cleaning process is guaranteed, the situation that the raw materials are stacked, and the overall cleaning effect is affected is avoided, the cleaning speed is high, and the cleaning efficiency is high; the water treatment device can treat water generated after raw materials are cleaned, guarantees recycling of water sources, and is simple in structure and easy and convenient to use.

The invention belongs to the technical field of nutritious food, discloses a soluble fiber solid beverage and a preparation method thereof, and solves the problems of single component, lack of functional synergy and insufficient universality of the existing soluble fiber solid beverage. The soluble fiber solid beverage is prepared from the following components in parts by weight: 1 to 2 parts of alginate oligosaccharide, 25 to 40 parts of resistant dextrin, 20 to 30 parts of inulin, 15 to 25 parts of polydextrose, 10 to 20 parts of fructo-oligosaccharide, 3 to 5 parts of stachyose and 0.1 to 0.3 part of stevioside. The total soluble fiber content can reach 88.5%, the dissolution time is only 2.3 s, the particle size of undissolved particles is smaller than or equal to 5 microns, the abdominal distension occurrence rate is 6.0%, the gas production rate is 10.0%, and the low-sugar-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low

A composition and a use thereof. The composition comprises brazzein, stevioside, and erythritol, wherein the mass ratio of brazzein to stevioside to erythritol is (2-48):(5-160):(5000-80000). The composition can be widely used in food and beverage products and daily chemical care products as an alternative of sucrose / other sweeteners, thereby reducing potential health risks caused by excessive intake of nutritive sweeteners.

The present invention discloses a method for producing a target stevioside. According to the method for producing the target stevioside, the distribution change amount of the target stevioside reaches 90% or above within 2 hours, so that a precipitate containing the target stevioside is formed. The target stevioside is rebaudioside D and / or rebaudioside M. The target stevioside is rebaudioside M.

The invention discloses a loratadine oral liquid and a production process thereof. Comprising the following raw materials in parts by weight: 0.5 to 0.6 part of loratadine, 5 to 6 parts of beta-cyclodextrin, 10 to 12 parts of polyethylene glycol 400, 8 to 10 parts of glycerol, 6 to 8 parts of propylene glycol, 0.3 to 0.4 part of sucralose, 0.2 to 0.3 part of stevioside, 15 to 18 parts of maltitol, 1 to 1.2 parts of citric acid, 0.5 to 0.6 part of malic acid, 0.1 to 0.15 part of disodium EDTA (Ethylene Diamine Tetraacetic Acid), 0.8 to 1 part of vitamin C, 0.3 to 0.4 part of xanthan gum, 0.5 to 0.6 part of sodium carboxymethyl cellulose and 0.4 to 0.5 part of lemon essence. The composition comprises the following components in parts by weight: 0.1-0.15 part of menthol, 2-3 parts of hawthorn extract, 1.2-1.5 parts of soya bean lecithin and the balance of purified water, totaling 100 parts. The loratadine is clathrated by the beta-cyclodextrin, the absorption promotion effect of the soya bean lecithin is combined, and the compound sweetening agent, the compound stabilizer and the natural flavor substance are matched, so that the problems of poor solubility and obvious bitter taste of the loratadine are solved, and the stability and bioavailability of the product are also improved.

The invention discloses high-solubility and high-stability protein powder containing an acidic functional component. The protein powder comprises the following components in percentage by mass: 70-92% of soybean protein isolate, 1-3% of the acidic functional component and 4.52-16.5% of a composite dissolution-promoting dispersion-stabilizing component, the composite dissolution-promoting and dispersion-stabilizing component is prepared from the following components in percentage by mass: 3 to 10 percent of isomaltooligosacharide, 1.5 to 6 percent of fructo-oligosaccharide and 0.02 to 0.5 percent of stevioside; the invention further discloses a preparation method of the high-solubility and high-stability protein powder containing the acidic functional components. The invention further discloses an acidic functional protein beverage and a preparation method thereof. The brewing dissolution speed and the solution stability are remarkably improved through an oligosaccharide-sweetening agent compounding system, the dry mixing process is simple, complex modification treatment is not needed, the cost is lower than that of an enzymolysis or microcapsule technology, and the good industrialization advantage is achieved.

The invention belongs to the technical field of food coloring, and particularly relates to a compound sweetening agent capable of coloring at low temperature for food as well as a preparation method and application of the compound sweetening agent. The D-psicose and tagatose chewable tablets are prepared from the following raw materials in parts by mass: 15 to 20 parts of D-psicose, 1 to 5 parts of tagatose, 2 to 8 parts of D-xylose, 20 to 25 parts of xylitol, 30 to 35 parts of solid maltitol, 30 to 35 parts of liquid maltitol, 10 to 14 parts of polydextrose and 0.5 to 1.5 parts of stevioside. The sweetening agent with a coloring function is perfectly combined with other sugar-free sweetening agents, so that function synergy is realized, food can quickly generate good color and luster in a low-temperature state, and the nutrition of the food is prevented from being lost.

The application belongs to the technical field of steviol glycoside production, and discloses a method for preparing steviol glycoside single components by using low total glycoside mother liquor sugar, which comprises the following steps: dissolving the low total glycoside mother liquor sugar in 80-95% methanol solution, crystallizing, filtering to obtain mother liquor and filter cake, treating the filter cake with 40-50% methanol solution, stirring and crystallizing, filtering to obtain mother liquor and filter cake, drying the filter cake to obtain pure rebaudioside A; using macroporous adsorption resin to separate the two mother liquors obtained in the previous step, using 30% ethanol solution to separate the glycosides adsorbed in the macroporous adsorption resin, playing a separating role, using 50% ethanol solution added with 1‰ oil tea saponin to resolve the macroporous adsorption resin, crystallizing and recrystallizing the resolution liquid, drying the filter cake to obtain rebaudioside C, and combining the two mother liquors obtained, removing impurities, and using high-pressure chromatography to collect resolution liquid containing rebaudioside F, and drying the resolution liquid to obtain rebaudioside F. The method is simple and effective, and greatly improves the reutilization rate of low total glycoside mother liquor sugar.

The invention belongs to the technical field of genetic engineering and functional enzyme preparations, particularly relates to sucrose synthase and a coding gene and a mutant thereof, and further discloses application of the sucrose synthase. According to the invention, sucrose synthase is obtained from candidate methylobacterium and is further mutated, so that a sucrose synthase mutant of which the enzyme activity, the conversion rate and the stability are remarkably improved is obtained, and the activity and the efficiency of a stevioside synthesis process are effectively improved.

The invention discloses siraitia grosvenorii and stevia rebaudiana flavored syrup and a preparation method thereof, and belongs to the field of food processing. The syrup is prepared from 72%-99% of water, 0%-26% of erythritol, 0%-1% of mogroside, 0%-0.2% of stevioside and citric acid lt. 0.3% of potassium sorbate; the mass ratio of the mogroside to the stevioside is (1-5): 1, and the pH value of the mogroside and the stevioside is 3.5-5.5. The preparation method comprises the steps of raw material preparation, mixing and stirring, pH value adjustment, sterilization treatment, preservative addition and packaging. Through synergistic compounding of the sweetening agent and process optimization, the problems that an existing product is not coordinated in flavor and poor in stability are solved, and the product is fresh, sweet, free of peculiar smell, long in storage life, suitable for the fields of beverages, baking and the like and good in application value.

The invention relates to a sweetening composition comprising a naturally sweet carbohydrate, a stevia compound, rubusoside, and tannin, wherein component d) is present in an amount on the scale of 1 mg / l to 400 mg / l; the invention also relates to a sweetening composition comprising a naturally sweet carbohydrate, a stevia compound, rubusoside, tannin, and oak extract or pomegranate extract; the invention further relates to a sweetening composition comprising acesulfame or aspartame, sucralose, a stevia compound, saccharin or cyclamate, neohesperidin, and tannin in an amount on the scale of 1 mg / l to 400 mg / l; furthermore, the invention relates to a sweetening composition comprising a) 0.1 to 50 g / l of a naturally sweet-tasting carbohydrate, b) 40 to 120 g / l of a naturally sweet-tasting carbohydrate other than a), and c) 30 to 300 mg / l of rubusoside; and, the invention relates to the use of said sweetening compositions for sweetening and preparing beverages.

Compositions and consumables comprising certain sweeteners and at least one flavonoid of Formula I are provided herein, wherein the at least one sweetener is selected from certain steviol glycoside and / or mogroside sweeteners. The at least one flavonoid enhances the sweetness of the consumable and / or modulates one or more taste attributes to make the consumable taste more like a sucrose-sweetened consumable. Methods of enhancing the sweetness of a consumable, methods of making a consumable taste more like a sucrose-sweetened consumable and methods of preparing consumables are also detailed herein.

The present invention relates to a preparation method for a stevia tomato and a stevia tomato prepared thereby. The preparation method of the present invention is to enhance sweetness of a tomato by using stevioside having strong sweetness with few calories, and thus may provide an alternative to consumers who want to reduce sugar intake, may enable uniform distribution of stevioside inside a tomato to maintain consistent taste throughout the tomato, may prevent quality degradation caused by oxidation by coating with vitamin C, which is an antioxidant, and may maintain freshness of a tomato for a long period of time. In addition, the stevia tomato prepared by the preparation method of the present invention has improved oxidation resistance as compared to conventional tomatoes and reduces problems such as discoloration and spoilage, thereby increasing the period for which freshness is maintained, which may contribute to extending the shelf life and increasing commercial value.

A novel steviol glycoside has been demanded. According to one aspect of the present invention, there is provided a compound represented by formula (1) (wherein (i) R1 represents Xyl(1-2)Glc1- and R2 represents Glc(1-2)Glc1-; or (ii) R1 represents Glc(1-2)Glc1- and R2 represents Glc(1-2)[Xyl(1-3)]Glc1- wherein Glc represents glucose and Xyl represents xylose), or a salt or hydrate thereof.

This invention discloses a method for recovering a mixture of steviol glycosides and rebaudioside B from stevia mother liquor, comprising the following steps: dissolving stevia mother liquor in purified water to obtain a stevia mother liquor solution; adding a pH adjuster to the stevia mother liquor solution to adjust the pH to 6.5-7.0, obtaining a solution to be treated; filtering the solution to be treated once using a hollow fiber membrane, and washing the retentate with purified water to obtain a retentate and a permeate; subjecting the permeate to vacuum concentration and spray drying sequentially to obtain a mixture of steviol glycosides; heating the retentate and adjusting the pH to 4.5-5.0, performing sedimentation treatment, filtering while hot, and drying the filter cake to obtain the rebaudioside B product. This invention uses stevia mother liquor as raw material to produce a mixture of steviol glycosides with high total glycoside content, rebaudioside B, and feed additives, improving the economic value of stevia mother liquor. The process is simple, easy to operate, and suitable for large-scale industrial production, with significant economic and social benefits.

This invention relates to the field of biosynthesis technology, and particularly to engineered yeast strains and their application in the total fermentation production of steviol glycosides. This invention utilizes site-directed mutagenesis to mutate wild-type kaurenoic acid 13-hydroxylase, obtaining the G481L / Q159L mutant. This mutant is then applied to the production of steviol or steviol glycosides, constructing a steviol or steviol glycoside synthesis pathway in the *Saccharomyces cerevisiae* host to achieve efficient production of steviol or steviol glycosides. Simultaneously, by enhancing the level of diterpene precursor synthesis genes in chassis cells (MVA pathway, acetyl-CoA, GGPP, transport proteins, UDPG pathway), the yield of steviol glycosides is further increased, reducing costs for industrial production.

The invention belongs to the technical field of medicinal preparation processing, and particularly relates to oral liquid for supplementing calcium, vitamin D and vitamin K and a preparation method of the oral liquid. The fruit and vegetable powder is prepared from the following raw materials in parts by weight: 100 to 500 parts of calcium citrate, 0.2 to 2.5 parts of vitamin D3 emulsion, 0.4 to 5.5 parts of vitamin K2, 0 to 100 parts of casein phosphopeptides, 0.1 to 200 parts of fruit and vegetable powder, 0 to 5 parts of citric acid, 0 to 5 parts of malic acid, 0.1 to 400 parts of a sweetening agent, 0.01 to 10 parts of a thickening agent, 0.1 to 5 parts of an emulsifying agent, 0 to 2 parts of stevioside and 665 to 1395 parts of water. According to the invention, the vitamin D3 emulsion process is improved, the proper antioxidant is added, and the proper consistency is adjusted, so that the stability of the vitamin D3 in the oral liquid product is ensured, and the dosage form application range of the calcium supplement is enlarged.

The present invention relates to a UDP-glucosyltransferase variant, a fusion enzyme of UDP-glucosyltransferase variants, and a use thereof for producing steviol glycoside. The UDP-glucosyltransferase variant and the fusion enzyme of UDP-glucosyltransferase variants according to the present invention have improved enzymatic activity. In addition, the fusion enzyme of UDP-glucosyltransferase variants has further improved enzymatic activity compared to when each variant is used individually. Accordingly, the UDP-glucosyltransferase variant and the UDP-glucosyltransferase variant fusion enzyme according to the present invention can be widely applied in the field of steviol glycoside synthesis, and have excellent commercial value and significance in that production efficiency can be increased and production costs can be reduced.