308 results about "Steviolmonoside" patented technology

The invention relates to the field of nutrient solutions, and particularly discloses a high-calcium-zinc dietary nutrient solution for promoting skeletal development and a preparation method thereof, the high-calcium-zinc dietary nutrient solution for promoting skeletal development comprises the following raw materials: calcium citrate, zinc citrate, a zinc-containing yeast extract, vitamin D3, a nutrition enhancer, functional oligosaccharide, xanthan gum, anhydrous citric acid, essence, a sweetening agent and water; the preparation method comprises the following steps: mixing the zinc citrate, the zinc-containing yeast extract, the seaweed meal, the natto powder, the stachyose and the trehalose to prepare a mixture A; uniformly premixing stevioside, xanthan gum and trehalose to obtain a mixture B; s4, vitamin D3 powder and trehalose are premixed and dissolved, and a mixture C is prepared; heating the residual water, adding the mixture A, the fructo-oligosaccharide, the anhydrous citric acid, the calcium citrate, the mixture B, the trehalose and the mixture C, adding the essence, and stirring to obtain the beverage. The preparation method has the characteristic of preparing the liquid calcium-zinc dietary nutrient solution with good absorption and utilization rate and palatability.

The invention discloses a preparation method of glucosyl stevioside, and relates to the technical field of glucosyl stevioside production, rebaudioside A or stevioside is taken as a raw material, cyclodextrin is taken as an auxiliary material, cyclodextrin glucosyltransferase is added for glycosylation reaction, a glucosyl stevioside solution I is cooled, and a glucosyl stevioside solution II is obtained; the method comprises the following steps: adding sucrose, UGT glycosyl transferase, sucrose synthase and uridine diphosphate into rebaudioside A or stevioside, continuously converting unreacted rebaudioside A or stevioside into rebaudioside D or rebaudioside E, inactivating after the reaction is finished, filtering by a plate frame, removing impurities through resin, analyzing the resin by using ethanol to obtain an analysis solution, and treating the analysis solution at 78-82 DEG C for 2-4 hours to obtain the rebaudioside D or rebaudioside E; and dealcoholizing at the vacuum degree of-0.1 MPa, concentrating and drying to obtain the glucosyl stevioside. The quality of the product is improved, the cost is reduced, and the taste of the product is integrally improved.

The invention relates to the technical field of image data processing, in particular to a stevioside impurity detection method based on industrial vision, which comprises the following steps: placing a supersaturated solution in a crystallization container for cooling crystallization to obtain surface images of each detection area under different illumination angles so as to determine a gray level fluctuation coefficient of each detection area; screening a feature detection area, acquiring a surface image of the feature detection area at a feature acquisition illumination angle to identify a feature contour, acquiring feature contours at different crystallization temperatures, determining a contour growth coefficient, determining a risk tendency category of the feature contour, and determining a mode of performing impurity marking; according to the method, a high-risk region where impurities exist is rapidly identified, the impurity marking mode is adaptively adjusted according to different crystal contour features in the surface image of the stevioside crystal, and the stevioside impurity detection reliability and detection efficiency are improved.

The invention belongs to the technical field of biosynthesis, and particularly relates to an engineering bacterium for preparing stevioside through de novo fermentation as well as a preparation method and application of the engineering bacterium. According to the invention, mevalonate kinase in the MVA pathway is mutated and optimized. The method comprises the following steps: carrying out point mutation on mevalonate kinase MvK of a wild type source, constructing a stevioside synthesis route in an escherichia coli host, and comparing the influence of MvK mutation on the yield of stevioside, so as to determine a Q160L mutant, and applying the Q160L mutant to the stevioside production route to realize efficient production of stevioside.

The invention discloses a method for preparing low-grafted glucosyl stevioside at high temperature and high pressure, which comprises the following steps: taking stevioside as a receptor, beta-cyclodextrin as a donor and CGT enzyme as a catalyst for reaction to obtain reaction liquid containing glucosyl stevioside; putting the reaction liquid containing the glucosyl stevioside into a high-pressure reaction kettle, and adding a catalyst to carry out high-temperature and high-pressure cracking reaction; after the high-temperature and high-pressure cracking reaction is finished, cooling a cracking solution to room temperature, filtering, concentrating for multiple times by adopting a polymeric membrane, and collecting a trapped fluid; and carrying out spray drying on the trapped fluid to obtain the low-grafting glucosyl stevioside. According to the method, firstly, glucosyl stevioside is prepared by adopting an enzyme modification method, then splitting decomposition is performed under certain conditions, and then a specific purification process is performed, so that the prepared product is high in stability, the proportion of low-grafted glucosyl stevioside in the product is high, and the product tastes good.

The invention belongs to the technical field of biosynthesis, and particularly relates to a method for regulating and controlling stevioside production through a UDPG synthesis route. According to the invention, a high-efficiency stevioside biosynthesis system is constructed through systematic engineering modification: in the aspect of chassis cell optimization, transcription factors (AtWRKY18, AtWRKY40 and AtMYC2) for regulating and controlling MEP pathway and key genes (dxs and / or dxr) of the MEP pathway are over-expressed, so that supply of terpene precursors is enhanced; and a heterologous MVA pathway (over-expression T7-atoB-mvaS-mvaE-mvK / mvKmut-pmK-mvaD-idi) with a modular design is introduced, so that sufficient supply of the terpenoid precursor is realized. Meanwhile, the intracellular concentration of the UDPG is increased by systematically enhancing a UDPG synthesis pathway (knocking out ugd, glgc, ushA, agp, deoA, tdk, udp, or thrA, and overexpressing galU, pgm, pyrE, pyrH, ugpA, or prs), so that the production capacity of the stevioside is remarkably improved.

The invention belongs to the technical field of biosynthesis, and particularly relates to a method for regulating and controlling stevioside production by improving the utilization rate of a carbon source. According to the invention, a high-efficiency stevioside biosynthesis system is constructed through systematic engineering modification: in the aspect of chassis cell optimization, transcription factors (AtWRKY18, AtWRKY40 and AtMYC2) for regulating and controlling MEP pathway and key genes (dxs and / or dxr) of the MEP pathway are over-expressed, so that supply of terpene precursors is enhanced; and a heterologous MVA pathway (over-expression T7-atoB-mvaS-mvaE-mvK / mvKmut-pmK-mvaD-idi) with a modular design is introduced, so that sufficient supply of the terpenoid precursor is realized. Meanwhile, by knocking out related genes and bypass metabolism genes (mgsA, iclR, menA or adhE) synthesized by organic acid, a glucose transport and utilization system (fnr, fadR, ppc, zwf or pgi is knocked out, and ACS, fbaA, tpiA, glf, galP or ptsG is overexpressed, so that the utilization rate of a carbon source is further improved, and the production capacity of stevioside is remarkably improved.

The invention relates to a stevioside composition, which comprises at least one stevioside and at least one of siamenoside I, mogroside V, mogroside III-E and mogroside II-E, and is characterized in that the stevioside composition comprises at least one stevioside and at least one of siamenoside I, mogroside V, mogroside III-E and mogroside II-E; wherein the stevioside has a higher solubility in the composition than in an equivalent composition lacking siamenoside I, mogroside V, mogroside III-E, and mogroside II-E. The invention also relates to a method for preparing the stevioside composition. The composition may be an aqueous solution.

The invention discloses a sports nutrition solid food for supplementing protein and a preparation method, and belongs to the technical field of foods.The sports nutrition solid food is prepared from, by weight, 31-60 parts of compound amino acid powder, 10-15 parts of isomaltitol, 1-6 parts of taurine, 1-5 parts of Chinese yam peptide, 5 parts of glycine, 5 parts of gamma-aminobutyric acid, 5 parts of L-lysine hydrochloride and 1 part of yeast protein powder. The formula comprises the following components in parts by weight: 1-3 parts of whey protein powder, 1-2 parts of hydroxymethyl propyl cellulose, 0.1-1 part of yeast extract, 0.1-1 part of yeast beta-glucan, 2-6 parts of bovine colostrum powder, 0.5 part of stevioside, 0.1 part of zinc glycinate, 0.1 part of ferrous lactate, 0.5 part of essence and 2-3 parts of magnesium stearate. The compound amino acid powder, the Chinese yam peptide, the glycine, the L-lysine hydrochloride, the yeast protein powder, the whey protein powder, the yeast extract and the bovine colostrum powder are compounded, so that protein consumed by a human body after exercise can be comprehensively supplemented.

The invention relates to the technical field of beverages, and particularly discloses a high-protein beverage containing psicose and a preparation method of the high-protein beverage. The high-protein beverage containing psicose is prepared from the following raw materials in percentage by mass: 13 to 17 percent of supplementary protein, 4 to 5 percent of an emulsifier, 3.10 to 3.14 percent of a flavoring agent, 0.02 to 0.06 percent of a thickening agent, 0.005 to 0.015 percent of a stabilizer, 0.008 to 0.01 percent of a coloring agent, 0.04 to 0.08 percent of an enzyme preparation and the balance of water, the flavoring agent is prepared from D-psicose, erythritol, sucralose and stevioside. The D-psicose in the flavoring agent is extremely low in calorie, does not cause increase of blood sugar and insulin, and has a unique taste, and the erythritol, the sucralose and the stevioside are matched, so that the taste of sucrose is reduced on the premise of low calorie, and the problem of bitter taste of a sugar substitute beverage is solved, so that the flavor of the protein beverage is improved.

The utility model discloses a recycling device of stevioside mother liquor, and relates to the field of stevioside production, an outlet of a crude product powder tank is communicated with a dissolving tank, an outlet of the dissolving tank is communicated with a first crystallizing tank, an outlet of the first crystallizing tank is communicated with a first separator, and the first separator is communicated with a first crystal tank and a first crystallizing mother liquor tank; an outlet of the first crystallization mother liquor tank is communicated with a second crystallization tank through a pipeline, an inlet of the second crystallization tank is communicated to the crude product powder tank, an outlet of the second crystallization tank is communicated with a second separator through a pipeline, a solid phase outlet of the second separator is communicated with a second dryer, and an outlet of the second dryer is communicated with a second crystal tank; and a liquid phase outlet of the second separator is communicated with a second crystallization mother liquor tank. The primary crystallization mother liquor is used as a solvent to dissolve a new material and then is subjected to secondary crystallization, so that the solvent and solvent recovery energy consumption are saved, meanwhile, the product yield is improved, the additional value of mother liquor sugar is increased, the cost is low, and the method is suitable for industrial production.

The invention relates to the technical field of functional beverages, in particular to a composite polypeptide solid beverage with a uric acid reducing function and a preparation method of the composite polypeptide solid beverage. The composite polypeptide solid beverage with the uric acid reducing function is prepared from the following raw materials in percentage by weight: 15-25% of plukenetia volubilis extract, 30-40% of lysimachia christinae hance-plantain herb composite extract, 8-12% of oyster peptide powder, 5-8% of earthworm protein peptide freeze-dried powder and 3-5% of a flavoring agent. Wherein the ratio of a lysimachia christinae hance extract to a plantain herb extract in the lysimachia christinae hance-plantain herb composite extract is 3: The flavoring agent is erythritol and / or stevioside, xanthine oxidase is inhibited by using flavonoid substances, GLUT9 expression is synergistically down-regulated by using bispyribaloside, PRPP synthetase activity is regulated by using zinc-rich peptide, uric acid micro-crystals are dissolved by using earthworm protein peptide, a multi-target action network of flavonoid-glycoside-bioactive peptide is achieved, and the effect of improving the bioavailability of the flavonoid-glycoside-bioactive peptide is achieved. And triple mechanisms of excretion promotion, generation inhibition and kidney protection are realized.

The invention provides a protein component composition with special medical application as well as a preparation method and application thereof, and relates to the field of formula food with special medical application. Wherein the protein component composition comprises protein and a sweetening agent; the protein is one or more of hydrolyzed whey protein powder, hydrolyzed casein and albumin peptide. The sweetening agent is one or more of white granulated sugar, glucose syrup, malt syrup, high fructose corn syrup, erythritol, xylitol, maltitol and stevioside. A plurality of components such as a stabilizer, mineral substances and multivitamins can also be added into the protein component composition. The finally obtained composition can be applied to preparation of formula food for special medical purposes, and the content of total protein, hemoglobin, albumin or transferrin in blood of patients suffering from malnutrition or insufficient protein intake is increased.

Disclosed herein are formulations (including sweet proteins and rare sugars) for improving the sweetness of low glycemic index rare sugars without altering the texture and flavor of the rare sugars and methods for improving the sweetness of rare sugars. More specifically, the present invention discloses methods for improving the sweetness of rare sugars without altering the sweetness profile, texture and flavor of the primary sugars. The invention also discloses the addition of Brazitame protein with liquid rare sugars at different temperatures during the synthesis of the rare sugars, before crystallization or before or after caramelization of the rare sugars, to increase sweetness. The Brazilian sweet protein increases the sweetness of rare sugars, but is not limited to D-psicose, D-tagatose, D-allose, isomaltulose, trehalose, sorbose, pinose, leucobiose and the like. The invention also provides for the evaluation of non-nutritional sweeteners (such as aspartame, acesulfame, sodium cyclamate, neotame, saccharin, sucralose, stevioside, etc.), as each has a limitation of taste quality if used with rare sugars. However, the use of Brazilian sweet protein does not alter the flavor and texture of rare sugars, and the taste profile matches sucrose.

The present invention relates to the technical field of food and its preparation, and proposes a functional lutein chewable tablet comprising the following components by weight: 10-20 parts lutein, 3-5 parts emulsifier, 30-50 parts vegetable oil, 50-60 parts filler, 2-4 parts steviol glycoside, 3-5 parts sodium carboxymethyl starch, and 2-4 parts magnesium stearate; the emulsifier is composed of starch octenylsuccinate, sodium stearoyl lactylate, and citric acid fatty acid glyceride. This technical solution solves the problem of poor stability of functional lutein chewable tablets in the prior art.

The invention relates to the technical field of biological catalysis engineering, in particular to a method for improving the solubility of stevioside Reb M and Reb D through enzyme catalysis modification, which comprises the following steps: dissolving Reb M and Reb D with the purity of more than or equal to 90% in a buffer solution with the pH value of 4.0-8.0, adding a non-starch glycosyl donor and composite glycosyl transferase according to the enzyme-substrate mass ratio of 0.05-0.5, and optimizing the enzyme addition sequence to realize specific site modification; reacting for 6-24 hours under the conditions that the pH is 5.5-6.0 and the temperature is 60-85 DEG C, and controlling the proportion of DP2-3 to be 60-80%; hPLC sampling is carried out every 4-6 hours, and the unreacted substrate is less than or equal to 20%; the solubility of the product is greater than or equal to 25g / L, and the sweet taste of the product is pure. According to the method, the solubility of Reb M / Reb D is improved, the structure of a directional glycosylation controlled product is realized, and pure sweetness is reserved; the reaction is mild and controllable, enzyme inactivation is simple and free of residues, industrialization is easy, and stevioside application upgrading is promoted.

The invention relates to chicken skin fruity qi and blood tonifying soft sweets and a making method thereof, and belongs to the technical field of health food. The invention provides chicken skin fruity qi and blood tonifying soft sweets. The chicken skin fruity qi and blood tonifying soft sweets are prepared from the following components in parts by mass: 37 to 99 parts of chicken skin juice, 15 to 30 parts of red dates, 10 to 25 parts of radix astragali seu hedysari, 5 to 15 parts of radix angelicae sinensis, 1 to 8 parts of radix ophiopogonis, 65 to 83 parts of gelatin, 15 to 25 parts of agar, 130 to 160 parts of xylitol, 0.1 to 0.6 part of stevioside, 0.1 to 0.6 part of mogroside, 1 to 8 parts of citric acid and 150 to 240 parts of water. The chicken skin fruity qi and blood tonifying soft sweets have the effects of tonifying qi and blood, resisting oxidation, reducing blood sugar, preventing decayed teeth, enhancing the immune regulation ability and the like, the variety of soft sweets is enriched, the market diversification requirements are met, high-quality development of special agricultural products can be promoted, the supply chain of the agricultural industry chain is prolonged, and the market prospect is wide. The method has profound significance on development of special agricultural products and economy.

The utility model discloses cleaning equipment for stevioside production, and relates to the technical field of stevioside preparation. The conveying mechanism is arranged in the cleaning frame and used for conveying stevioside raw materials, the conveying mechanism comprises a driving part and a conveying belt, the driving part is arranged in the cleaning frame, the conveying belt is arranged on the driving part, and a plurality of conveying plates are fixedly connected to the conveying belt at equal intervals; and the cleaning mechanism is arranged in the cleaning frame and is used for cleaning the stevioside raw material. According to the cleaning equipment for stevioside production, the conveying mechanism is matched with the cleaning mechanism for use, raw materials of stevioside are dispersed, the effect of the cleaning process is guaranteed, the situation that the raw materials are stacked, and the overall cleaning effect is affected is avoided, the cleaning speed is high, and the cleaning efficiency is high; the water treatment device can treat water generated after raw materials are cleaned, guarantees recycling of water sources, and is simple in structure and easy and convenient to use.

The present invention provides a Stevia plant having a low ability to form pollens as compared with the wild type Stevia species. The present invention also provides a method of producing such a Stevia plant having a low ability to form pollens, and an extract or a steviol glycoside purified product obtainable from such a plant.

Consumables comprising cyclamate and a steviol glycoside sweetener or mogroside sweetener are provided herein, wherein cyclamate, when present in an amount at or below its sweetness recognition threshold concentration, enhances the sweetness of and, optionally, improves the sweetness profile the sweetener, thereby providing a consumable that tastes more like a sucrose-sweetened consumable. Methods of enhancing the sweetness of a consumable, methods of making a consumable taste more like a sucrose-sweetened consumable and methods of preparing consumables are also detailed herein.

The invention belongs to the technical field of nutritious food, discloses a soluble fiber solid beverage and a preparation method thereof, and solves the problems of single component, lack of functional synergy and insufficient universality of the existing soluble fiber solid beverage. The soluble fiber solid beverage is prepared from the following components in parts by weight: 1 to 2 parts of alginate oligosaccharide, 25 to 40 parts of resistant dextrin, 20 to 30 parts of inulin, 15 to 25 parts of polydextrose, 10 to 20 parts of fructo-oligosaccharide, 3 to 5 parts of stachyose and 0.1 to 0.3 part of stevioside. The total soluble fiber content can reach 88.5%, the dissolution time is only 2.3 s, the particle size of undissolved particles is smaller than or equal to 5 microns, the abdominal distension occurrence rate is 6.0%, the gas production rate is 10.0%, and the low-sugar-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low-fat-content low

The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with β-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided.

A composition and a use thereof. The composition comprises brazzein, stevioside, and erythritol, wherein the mass ratio of brazzein to stevioside to erythritol is (2-48):(5-160):(5000-80000). The composition can be widely used in food and beverage products and daily chemical care products as an alternative of sucrose / other sweeteners, thereby reducing potential health risks caused by excessive intake of nutritive sweeteners.

The present invention discloses a method for producing a target stevioside. According to the method for producing the target stevioside, the distribution change amount of the target stevioside reaches 90% or above within 2 hours, so that a precipitate containing the target stevioside is formed. The target stevioside is rebaudioside D and / or rebaudioside M. The target stevioside is rebaudioside M.

Methods of preparing steviol glycosides are described. The methods include utilizing enzyme preparations and recombinant microorganisms for converting various starting compositions to target steviol glycosides. The steviol glycosides are useful as non- caloric sweetener, flavor enhancer, sweetness enhancer, flavor stabilizer, flavoring with modifying properties (FMP), foaming suppressor and solubility enhancing agent in consumable products such as any food, beverages, pharmaceutical compositions, tobacco products, nutraceutical compositions, and oral hygiene compositions.

The invention discloses a loratadine oral liquid and a production process thereof. Comprising the following raw materials in parts by weight: 0.5 to 0.6 part of loratadine, 5 to 6 parts of beta-cyclodextrin, 10 to 12 parts of polyethylene glycol 400, 8 to 10 parts of glycerol, 6 to 8 parts of propylene glycol, 0.3 to 0.4 part of sucralose, 0.2 to 0.3 part of stevioside, 15 to 18 parts of maltitol, 1 to 1.2 parts of citric acid, 0.5 to 0.6 part of malic acid, 0.1 to 0.15 part of disodium EDTA (Ethylene Diamine Tetraacetic Acid), 0.8 to 1 part of vitamin C, 0.3 to 0.4 part of xanthan gum, 0.5 to 0.6 part of sodium carboxymethyl cellulose and 0.4 to 0.5 part of lemon essence. The composition comprises the following components in parts by weight: 0.1-0.15 part of menthol, 2-3 parts of hawthorn extract, 1.2-1.5 parts of soya bean lecithin and the balance of purified water, totaling 100 parts. The loratadine is clathrated by the beta-cyclodextrin, the absorption promotion effect of the soya bean lecithin is combined, and the compound sweetening agent, the compound stabilizer and the natural flavor substance are matched, so that the problems of poor solubility and obvious bitter taste of the loratadine are solved, and the stability and bioavailability of the product are also improved.

Methods of preparing highly purified steviol glycosides. The methods include utilizing enzyme preparations and recombinant microorganisms for converting various starting compositions to target steviol glycosides. The highly purified steviol glycosides are useful as non-caloric sweetener, flavor enhancer, sweetness enhancer, and foaming suppressor in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.

The invention discloses high-solubility and high-stability protein powder containing an acidic functional component. The protein powder comprises the following components in percentage by mass: 70-92% of soybean protein isolate, 1-3% of the acidic functional component and 4.52-16.5% of a composite dissolution-promoting dispersion-stabilizing component, the composite dissolution-promoting and dispersion-stabilizing component is prepared from the following components in percentage by mass: 3 to 10 percent of isomaltooligosacharide, 1.5 to 6 percent of fructo-oligosaccharide and 0.02 to 0.5 percent of stevioside; the invention further discloses a preparation method of the high-solubility and high-stability protein powder containing the acidic functional components. The invention further discloses an acidic functional protein beverage and a preparation method thereof. The brewing dissolution speed and the solution stability are remarkably improved through an oligosaccharide-sweetening agent compounding system, the dry mixing process is simple, complex modification treatment is not needed, the cost is lower than that of an enzymolysis or microcapsule technology, and the good industrialization advantage is achieved.

The invention discloses low-sugar chocolate, low-sugar chocolate crispy ice cream and a preparation method thereof, and relates to the technical field of food. Total cocoa solids, sugar alcohol raw materials, trehalose, an emulsifier and stevioside are used as main raw materials, white granulated sugar is replaced by the sugar alcohol raw materials, and trehalose and stevioside are matched, so that the chocolate meets the low-sugar requirement by adjusting the dosage of each component, the aftertaste of cocoa can be well balanced, and the taste of the chocolate is improved. Meanwhile, the two technical problems of low sugar and taste are solved.