41 results about "Steviolmonoside" patented technology

A novel steviol glycoside has been demanded. According to one aspect of the present invention, there is provided a compound represented by formula (1) (wherein (i) R1 represents Xyl(1-2)Glc1- and R2 represents Glc(1-2)Glc1-; or (ii) R1 represents Glc(1-2)Glc1- and R2 represents Glc(1-2)[Xyl(1-3)]Glc1- wherein Glc represents glucose and Xyl represents xylose), or a salt or hydrate thereof.

This invention relates to the field of biosynthesis technology, and particularly to engineered yeast strains and their application in the total fermentation production of steviol glycosides. This invention utilizes site-directed mutagenesis to mutate wild-type kaurenoic acid 13-hydroxylase, obtaining the G481L / Q159L mutant. This mutant is then applied to the production of steviol or steviol glycosides, constructing a steviol or steviol glycoside synthesis pathway in the *Saccharomyces cerevisiae* host to achieve efficient production of steviol or steviol glycosides. Simultaneously, by enhancing the level of diterpene precursor synthesis genes in chassis cells (MVA pathway, acetyl-CoA, GGPP, transport proteins, UDPG pathway), the yield of steviol glycosides is further increased, reducing costs for industrial production.

The utility model discloses a kind of multi-enzyme step-by-step preparation glucosyl steviol glycoside's device, it is related to glucose steviol glycoside production technical field, the inlet of first reaction tank is respectively communicated with raw material tank, dextrin tank, cyclodextrin glucoside transferase tank and first sodium hydroxide solution tank, the outlet of first reaction tank is communicated with hydrolysis tank, the outlet of hydrolysis tank is communicated with second reaction tank, the inlet of second reaction tank is respectively communicated with sucrose tank, sucrose synthase tank, uridine diphosphate tank, UGT glycosyltransferase tank and lye tank, the outlet of second reaction tank is communicated with concentration tank by pipeline, the lower outlet of concentration tank is communicated with drier, the outlet of drier is communicated with glucosyl steviol glycoside tank. Make residual substrate conversion is relatively more optimal steviol glycoside in taste, improve the taste, quality and yield of product.

The present invention relates to novel steviol glycosides R6-1, R6-2A, R6-2B, R6-4A, R6-4B and R7-2 and the production of these novel steviol glycosides, such as through enzymatic bioconversion. The use of these novel steviol glycosides as sweeteners and in orally consumable products are also provided.

This invention relates to a novel Lactobacillus mari strain exhibiting excellent glucose transfer activity and a method for producing glucose-transferable steviol glycosides using the same. The novel Lactobacillus mari CJST01 strain exhibits superior steviol glycoside transfer activity compared to Lactobacillus mari DSM20444 strain, which is known to produce glucose-transferable steviol glycosides. This results in higher productivity and a shorter enzymatic reaction time in the manufacturing process. The steviol glycosides produced using this strain have the advantage of superior taste quality and aroma compared to alpha-1,4 linked steviol glycosides and rebaudioside M. Furthermore, glucose-transferable rebaudioside A produced using Lactobacillus mari CJST01 strain exhibits reduced off-flavors and odors and superior solubility.

To provide a taste-modifying composition that reduces the saltiness of sodium salts, such as sodium bicarbonate; the use of such a taste-modifying composition to reduce the saltiness of sodium salts; and a composition (such as an edible composition or an oral care composition) comprising a sodium salt and a taste-modifying composition. [Solution] below: (a) Glucosylated natural steviol glycoside; (b) 4-amino-5,6-dimethylthieno[2,3-d]pyrimidine-2(1H)-one or a salt thereof; (c) sucralose; and (d) Oxacyclohexadecane-2-one A taste-modifying composition comprising two or more combinations of the following.

This invention discloses a method for preparing high-purity steviol glycosides using enzyme-catalyzed membrane separation, specifically relating to the field of process control technology for enzyme-membrane co-separation of functional sweeteners. It addresses the problems of existing steviol glycoside enzyme-catalyzed membrane separation processes being highly dependent on manual experience, exhibiting large fluctuations in product composition due to variations in raw materials and membrane conditions, and difficulty in consistently obtaining high-purity, high-order steviol glycoside concentrates. By constructing an enzyme-membrane co-separation operating condition fingerprint for each batch, encompassing raw materials, enzymes, membranes, and the target product grade, and generating target glycoside composition trajectories, membrane operation constraints, and phased operation strategies based on this fingerprint, and combining online status acquisition and real-time deviation judgment for closed-loop linkage control, this method achieves stable convergence to the predetermined high-purity, high-order steviol glycoside product composition even with fluctuations in raw material quality and membrane conditions. This solves the technical problems of large product composition fluctuations and reliance on repeated trial and error in existing enzyme-catalyzed membrane separation processes.

The present invention relates to a method for producing steviol glycosides of Stevia rebaudiana, which method comprises the following steps: preparing the Stevia rebaudiana nodal explants having two internodes; propagating the explants in temporary immersion systems (SETIS) with controlled immersion cycles; adding an elicitor after 21 days of culture; and transferring to a liquid culture medium enriched with growth regulators and nutrients that are essential to plant growth and development.

This invention relates to the field of traditional Chinese medicine preparations, specifically a chewable tablet for adjuvant treatment of myopia and its preparation method, comprising the following raw materials and excipients in parts by weight: Codonopsis pilosula 150-220 parts, Angelica sinensis 150-220 parts, Lycium barbarum 300-500 parts, Poria cocos 40-70 parts, Ophiopogon japonicus 50-80 parts, Alisma plantago-aquatica 35-50 parts, Schisandra chinensis 30-40 parts, Acorus tatarinowii 30-40 parts, Paeonia suffruticosa 35-50 parts, and Polygala tenuifolia (processed with licorice water) 30-40 parts; excipients: mannitol 120-160 parts, sucrose 220- 300 parts, 1-3 parts steviol glycosides, 3-5 parts magnesium stearate, 30-35 parts gastric-soluble film-coating premix, and an appropriate amount of 40-60% ethanol; This invention achieves the effects of tonifying qi and blood, nourishing the liver and kidneys through traditional Chinese medicines such as Codonopsis pilosula, Angelica sinensis, and Lycium barbarum, combined with the effects of Acorus tatarinowii and Polygala tenuifolia in opening the orifices and calming the mind. It has a significant effect on improving vision and relieving eye muscle spasms, especially for myopia, particularly pseudomyopia and eye fatigue in adolescents. The formulation of this invention strictly follows the traditional treatment principles of tonifying qi and blood, nourishing the liver and kidneys, and improving vision and calming the mind. The effective ingredient content is clear and the effect is stable.

The subject of the application is a dry mix for the production of a sugar-free confectionery product with an increased content of bioactive ingredients, especially muffins, in a mixture with liquid ingredients which are water and a fat component, wherein the dry mix contains egg powder capable of rehydration, flour, a raising and sweetening agent, citric acid in powder form, edible salt and mammalian milk powder, which is characterized in that it constitutes from 50% to 58% by weight. a confectionery product before baking, wherein the flour constitutes 36% - 38% by weight, wherein the flour is at least wheat flour and / or at least chickpea flour, egg powder constitutes from 1.5% to 3% by weight, and citric acid in powder form constitutes 0.1% - 0.5% by weight, the raising agent constitutes 0.4% - 2.5% by weight, the sweetener constitutes 6% - 15% by weight, the mammalian milk powder constitutes 1.0% - 3.0% by weight., edible salt constitutes 0.1% - 0.5% by weight, wherein the sweetener is maltitol and / or xylitol and / or a mixture of stevia with erythritol, wherein steviol glycosides constitute from 0.5% to 1% by weight. In turn, the sum of the dry mix and liquid ingredients of the confectionery product before baking is 100%, wherein the liquid ingredients constitute 42% - 50% by weight of the confectionery product before baking, wherein the fat component constitutes 12% - 15% by weight, and water constitutes 29% - 36% by weight. The fat component is in the form of sunflower oil and / or oil from berry seeds obtained by extraction using supercritical CO2 or a mixture thereof. The application also covers a method for obtaining a sugar-free confectionery product with an increased content of bioactive ingredients, especially muffins as defined above.

Steviol glycoside solubility enhancer, aqueous steviol glycoside solution, and non-alcoholic beverage. The present invention relates to a steviol glycoside solubility enhancer that may include a monocaffeoylquinic acid (MCQ) component comprising at least one compound selected from the group consisting of chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid and salts thereof; and a dicaffeoylquinic acid (DCQ) component comprising at least one compound selected from the group consisting of 1,3-dicaffeoylquinic acid, 1,4-dicaffeoylquinic acid, 1,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid and salts thereof.In a suitable implementation, the mcq component and the dcq component together comprise more than 50% (by weight), preferably more than 60% (by weight), more than 70% (by weight), more than 80% (by weight), more than 90% (by weight), or more than 95% (by weight) of the steviol glycoside solubility enhancer.

The invention disclosed herein relates generally to the field of recombinant production of a steviol glycoside, to the field of bioconversion of steviol into a steviol glycoside and to the field of bioconversion of a steviol glycoside into a further steviol glycoside. Particularly, the invention provides a process for recombinant production of a steviol glycoside, a process of bioconversion of steviol into a steviol glycoside, a process for bioconversion of a steviol glycoside into a further steviol glycoside and a composition comprising a steviol glycoside. More particularly, the invention relates to a microorganism that has a deficiency of a serine / threonine protein kinase and comprises a polynucleotide encoding a polypeptide having uridine diphosphate-dependent glucosyltransferase (UGT) activity.

The application discloses a compound flocculating agent and application thereof, and belongs to the technical field of flocculating agents. The compound flocculating agent comprises flocculating agent A and flocculating agent B. The flocculating agent A comprises attapulgite and bamboo charcoal, and the flocculating agent B comprises chitosan quaternary ammonium salt and polyacrylamide. The compound flocculating agent is applied to the flocculation of stevia extract liquid, and has the advantages of fast flocculation settling speed, low cost, simple operation, low loss rate of steviol glycosides, high clarification rate and good flocculation and impurity removal effects.

The application discloses an easily-absorbed food health-care medicament for the middle-aged and old people of a tonifying type and a preparation method thereof, which comprises the following raw materials in parts by weight: 15-30 parts of Astragalus membranaceus, 20-40 parts of Dioscorea opposita, 10-25 parts of Poria cocos, 5-15 parts of Angelica sinensis, 10-25 parts of Ziziphus jujuba, 10-20 parts of Lycium barbarum, 5-15 parts of Canarium album, 5-15 parts of Eucommia ulmoides, 10-20 parts of Galla rhois, 5-15 parts of fructooligosaccharide, 10-25 parts of malt dextrin, 2-8 parts of hydroxypropyl methyl cellulose, 1-5 parts of magnesium stearate, 0.5-3 parts of stevioside, 3-10 parts of citrus peel powder and 2-8 parts of licorice powder. The application constructs a multi-component synergistic tonifying formula system according to the physiological characteristics of the middle-aged and old people. The tonifying main materials cover multiple functions such as qi and blood tonifying, spleen invigorating and kidney benefiting, the components are mild and have synergistic effects, the application is suitable for the body conditioning needs of the middle-aged and old people, and the efficacy limitation of single component is avoided.

The application provides a composition with improved gastrointestinal function and a preparation method thereof, and belongs to the technical field of compositions.The composition comprises the following components in percentage by weight: fructo-oligosaccharides 25-45%, anhydrous magnesium citrate 20-30%, mannitol 10-20%, fruit powder 10-15%, guar gum 5-10%, konjac gum 1-5%, colloidal silicon dioxide 0.5-1% and steviol glycoside 0.1-0.5%; the application adopts a fermentation process on fructo-oligosaccharides, guar gum and konjac gum to obtain fermentation metabolites, the fermentation metabolites have a chemical stimulation peristalsis and combine the osmotic laxative of magnesium salt and the microecological regulation effect of prebiotics, and the pain points of traditional fiber preparations, such as sticky taste and slow effect, are solved.

The present disclosure relates generally to malonyl steviol glycosides and the use of such compounds for various comestible uses, such as sweetening a flavored product or enhancing the sweetness of another sweetener. In some aspects, the disclosure provides certain compositions that include such malonyl steviol glycosides, such as compositions that include such malonyl steviol glycosides and one or more other sweeteners. In some other aspects, the disclosure provides methods of reducing the caloric content of a flavored article, such as a flavored food or beverage product, or an oral care product.

Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express recombinant genes encoding UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol glycosides, e.g., Rebaudioside A and / or Rebaudioside D, which can be used as natural sweeteners in food products and dietary supplements.

An aqueous steviol glycoside solution comprises 5% by weight or more of one or more steviol glycosides and an amount of choline chloride such that the weight ratio of choline chloride to water is between 4 and 7. One or more steviol glycosides and choline chloride may be present in a weight ratio of 0.65 or less. One or more steviol glycosides and water may be present in a weight ratio of 4 or less. One or more steviol glycosides may comprise leboside D and leboside M. A method for increasing the solubility of a steviol glycoside composition comprises adding one or more steviol glycosides and choline chloride to an aqueous solution to form a steviol glycoside composition wherein the weight ratio of choline chloride to water is in the range of 4 to 7.

Provided is a novel beverage containing a steviol glycoside and an alcohol. One embodiment of the present invention provides a beverage that contains one or more steviol glycosides selected from the group consisting of rebaudioside A, rebaudioside D and rebaudioside M and has an alcohol content of 0.05 v / v% or more and less than 0.5 v / v%.

This invention relates to a process for improving the undesirable flavors of stevia based on liquid fermentation of shiitake mushrooms. This process can reduce the bitterness and grassy taste of stevia while retaining its sweetness. A composite culture medium is constructed using a specific ratio of potato flour, carrot flour, and stevia powder, and shiitake mushrooms are inoculated for liquid fermentation. The preparation steps include: dissolving, filtering, and sterilizing the composite culture medium in a water bath, then inoculating shiitake mushroom seed liquid in the logarithmic growth phase, and cultivating it in a shaker at a specific temperature to obtain a fermentation broth; this fermentation broth, after edible treatment, yields the flavor-improved product. This invention achieves the directional conversion of steviol glycosides through fermentation: while efficiently retaining the core sweet component ribobadiol A, it significantly reduces the content of steviol glycosides and ribobadiol C, and significantly enriches ribobadiol F, which has a sweetening effect. The final flavor-improved product shows a 33.29% reduction in bitterness and a 35.98% reduction in grassy taste, with a sweetness retention rate exceeding 88%.

Steviol glycoside composition, beverage, methods for reducing an undesirable sensory attribute of an aqueous solution of steviol glycoside, for reducing the sweetness persistence of a steviol glycoside component in an edible composition and for reducing the bitterness of a steviol glycoside component in an edible composition, and aqueous solutions of steviol glycoside with reduced sweetness persistence and aqueous solutions of steviol glycoside with reduced bitterness. The present invention relates to a sensory modifier that can provide a steviol glycoside composition with modified sensory attributes, for example, reduced sweetness persistence or reduced bitterness. This type of sensory modifier includes a salt fraction and an acid fraction. The salt fraction may include a salt of a monocaffeoylquinic acid and / or a salt of a dicaffeoylquinic acid.The acid fraction may include monocaffeoylquinic acid and / or dicaffeoylquinic acid. The salt fraction is preferably at least 50% by weight of sensory modifier.

This invention discloses a compound feed additive and its preparation method, belonging to the field of feed technology. The preparation method includes the following steps: mixing a bacterial liposome suspension, a polysaccharide extract, modified steviol glycosides, and an emulsifier in a weight ratio of 1:0.03–0.4:0.1–0.2:0.01, and stirring at 30°C for 20–24 hours to obtain a microcapsule emulsion; the microcapsule emulsion is then spray-dried to obtain the compound feed additive. This invention uses Bacillus subtilis and Bifidobacterium animalis as the core strains in the compound feed additive, preparing them into a bacterial liposome suspension, then mixing and reacting it with the polysaccharide extract, modified steviol glycosides, and emulsifier, and finally spray-drying it at high temperature to obtain a compound feed additive that improves feed intake and weight gain.

This invention discloses a production apparatus for preparing low-grafted glucosyl steviol glycosides, comprising a reaction unit, a pyrolysis unit, a cooling unit, a membrane purification unit, and a drying unit. The reaction unit includes an enzyme reaction vessel and enzyme solution storage tanks, steviol glycoside solution storage tanks, and β-cyclodextrin solution storage tanks connected to the enzyme reaction vessel. The pyrolysis unit includes a pyrolysis vessel connected to the enzyme reaction vessel. The cooling unit includes a cooling tank connected to the pyrolysis vessel. The membrane purification unit includes a membrane concentration tank connected to the pyrolysis vessel, which is divided into an upper chamber and a lower chamber by a polyamide membrane; the upper chamber is connected to a purified water storage tank. The drying unit includes a spray drying tower connected to the lower chamber and a product storage tank connected to the spray drying tower. This apparatus improves the conversion efficiency of raw materials to low-grafted glucosyl steviol glycosides through enzyme catalysis and pyrolysis, and increases the content of low-grafted glucosyl steviol glycosides in the product through subsequent purification processes.

The application discloses a multi-component effervescent tablet with natural eutectic solvent as components and separation effect on protein. The hydrogen bond donor of the natural eutectic solvent is glycerol, and the hydrogen bond acceptor includes mogroside or stevioside; the carbon dioxide source of the effervescent tablet is sodium carbonate or sodium bicarbonate, and the acid source is citric acid, tartaric acid or fumaric acid. The above natural components are mixed and heated to form the natural eutectic solvent with effervescence, which is then added to the raw powder of the effervescent tablet composed of the carbon dioxide source and the acid source, and the multi-component effervescent tablet loaded with the natural eutectic solvent is obtained by using the pressurization method. The process is simple, fast and low in cost, and is convenient for realizing large-scale production; the prepared effervescent tablet has ideal properties and hardness, small tablet weight difference, and obvious effect in enriching trace protein in the extract of snake slough. The application not only expands the existing use of the eutectic solvent, but also provides a new choice for the separation and enrichment of biological macromolecules.

This invention relates to a selenium-containing compound vitamin chewable tablet and its preparation method, comprising the following raw materials in parts by weight: 10-16 parts selenium-enriched yeast, 25-35 parts L-ascorbic acid, 0.5-1 part vitamin K, 10-20 parts sorbitol, 1-3 parts DL-α-tocopherol acetate, 3-8 parts sodium octenyl succinate starch, 1-5 parts acetylated distarch adipate, 0.7-1.2 parts magnesium stearate, 0.3-0.5 parts potassium citrate, 0.04-0.07 parts steviol glycosides, and 5-10 parts modified silica. This invention improves the crispness and palatability of the chewable tablet by adding acetylated distarch adipate and sodium octenyl succinate starch; the addition of modified silica effectively reduces vitamin attenuation and improves the stability of the components.

This invention discloses a compound taste-masking composition for leech decoction, the taste-masking leech decoction, its preparation method, and its application, belonging to the field of traditional Chinese medicine pharmaceutical technology. This invention provides a compound taste-masking composition composed of adenosine monophosphate (AMP), hydroxypropyl-β-cyclodextrin (HP-β-CD), and steviol glycosides, and provides its application method: first, HP-β-CD is added for inclusion and deodorization, then AMP and steviol glycosides are added to synergistically suppress bitterness. This combination utilizes a complementary strategy of "physical inclusion-receptor antagonism-taste neutralization" to simultaneously and efficiently mask the bitterness and fishy taste of leech decoction. Through orthogonal experimental optimization and verification using electronic tongue and sensory evaluation, the optimal formula (AMP 0.10%, HP-β-CD 0.50%, steviol glycosides 0.03%) reduces the bitterness score from 9.3 to 0.6 and the fishy taste score from 9.6 to 1.0. The method of this invention is performed at room temperature and pressure, is simple in process, does not affect the main active ingredients, is suitable for industrial production, and can significantly improve patients' compliance with leech decoction.

The application discloses a high-solubility steviol glycoside solution and a preparation method and application thereof. The high-solubility steviol glycoside solution comprises steviol glycoside and a water-soluble solubilizing substance solution; the mass molar concentration of the solubilizing substance in the water-soluble solubilizing substance solution is greater than 0 to solubility; the mass percentage concentration of the steviol glycoside in the high-solubility steviol glycoside solution is solubility in pure water to solubility in the water-soluble solubilizing substance solution; and the solubilizing substance comprises at least one of lactic acid, malic acid, citric acid and tartaric acid. The steviol glycoside is added into the water-soluble solubilizing substance solution, and after simple mixing and dissolving, the corresponding high-solubility steviol glycoside solution can be obtained. The steviol glycoside complex has the advantages of high solubility, high stability, low cost, simple preparation process and the like, is easy to produce on a large scale, and is suitable for liquid concentrate systems such as table sweeteners or beverage syrups.

A steviol glycoside solubility enhancer may include a monocaffeoylquinic (MCQ) component that includes at least one compound selected from the group consisting of chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, and salts thereof; and a dicaffeoylquinic (DCQ) component that includes at least one compound selected from the group consisting of 1,3-dicaffeoylquinic acid, 1,4-dicaffeoylquinic acid, 1,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, and salts thereof. In one suitable implementation, the MCQ component and the DCQ component together comprise more than 50% (wt), preferably more than 60% (wt), more than 70% (wt), more than 80% (wt), more than 90% (wt), or more than 95% (wt) of the steviol glycoside solubility enhancer.

The utility model discloses a production system of improving low grafting glucose base steviol glycoside content, including intercommunication enzymolysis reaction unit, high temperature cleavage unit, first separation unit, resin purification unit, first drying unit, crystallization unit, second separation unit, second drying unit, storage unit, enzymolysis reaction unit includes enzyme reaction jar, with the glycoside acceptor storage tank of enzyme reaction jar intercommunication, glycoside donor storage tank and enzyme preparation storage tank, high temperature cleavage unit includes, cleavage jar, resin purification unit includes first resin column, second resin column that intercommunication in proper order, and second resin column is connected with the analysis agent storage tank, crystallization unit includes the dissolving jar that communicates with first drying unit and the crystallization jar that communicates with the dissolving jar, and the dissolving jar is connected with the crystallization solvent storage tank. This production system passes through enzymolysis, high temperature cleavage conversion, two -stage resin selective enrichment and recrystallization refining, and the purity and yield of low grafting glucose base steviol glycoside in product have been improved significantly.