5 results about "Rebaudioside C" patented technology

The application belongs to the technical field of steviol glycoside production, and discloses a method for preparing steviol glycoside single components by using low total glycoside mother liquor sugar, which comprises the following steps: dissolving the low total glycoside mother liquor sugar in 80-95% methanol solution, crystallizing, filtering to obtain mother liquor and filter cake, treating the filter cake with 40-50% methanol solution, stirring and crystallizing, filtering to obtain mother liquor and filter cake, drying the filter cake to obtain pure rebaudioside A; using macroporous adsorption resin to separate the two mother liquors obtained in the previous step, using 30% ethanol solution to separate the glycosides adsorbed in the macroporous adsorption resin, playing a separating role, using 50% ethanol solution added with 1‰ oil tea saponin to resolve the macroporous adsorption resin, crystallizing and recrystallizing the resolution liquid, drying the filter cake to obtain rebaudioside C, and combining the two mother liquors obtained, removing impurities, and using high-pressure chromatography to collect resolution liquid containing rebaudioside F, and drying the resolution liquid to obtain rebaudioside F. The method is simple and effective, and greatly improves the reutilization rate of low total glycoside mother liquor sugar.

The application belongs to the technical field of chromatographic detection, and specifically discloses a method for simultaneously determining caffeine and various sweeteners in new tea drinks by high performance liquid chromatography. The application first prepares a sample of the new tea drink; then extracts the sample by ultrasonic wave to obtain a sample to be detected; and then detects the components to be detected in the sample to be detected by using specific high performance liquid chromatography conditions, and obtains the content of the components to be detected through post-processing; the components to be detected include caffeine, acesulfame potassium, sodium saccharin, aspartame, alitame, advantame, neotame, stevioside, rebaudioside A and rebaudioside C. The testing method disclosed by the application can simultaneously determine caffeine and various sweeteners in new tea drinks, improves the detection efficiency, reduces the detection cost, prevents and controls the food safety risk, and promotes the standardization system construction of new tea drinks.

The application discloses a method for separating and purifying rebaudioside F and rebaudioside C from stevia mother liquor sugar, and relates to the technical field of glycoside separation, aiming at solving the technical problem of high difficulty in separating and purifying rebaudioside F and rebaudioside C from stevia mother liquor sugar. The method for separating and purifying rebaudioside C from stevia mother liquor sugar comprises the following steps: diluting stevia mother liquor sugar, adsorbing the dilute solution on a macroporous adsorption resin, and obtaining a first eluate by elution; concentrating and drying the first eluate, dissolving the dried mixture in high-concentration methanol, and filtering to obtain a first filtrate; concentrating the first filtrate, diluting the concentrated solution with methanol, passing the dilute solution through a normal-phase low-pressure liquid chromatography column, and obtaining a second eluate by elution; and concentrating and drying the second eluate, dissolving the dried mixture in methanol, and filtering to obtain a second filter cake containing rebaudioside C. The technical scheme provided by the application is used for separating and purifying rebaudioside F and rebaudioside C from stevia mother liquor sugar.

The application discloses a method for continuously separating rebaudioside C and rebaudioside F from stevioside mother liquor sugar by using a double analog moving bed, relates to the technical field of stevioside production, and discloses the following steps: stevioside mother liquor sugar solution enters a first-stage SMB (simulated moving bed) analog moving bed system, is eluted by gradient mobile phases formed by deionized water and 30-50 vol % of an ethanol aqueous solution, and mixed enrichment liquid rich in rebaudioside C and rebaudioside F is collected; after dealcoholization of the mixed enrichment liquid, crystallization and solid-liquid separation are carried out, and rebaudioside C crystals and a crystallization mother liquor are obtained; the crystallization mother liquor enters a second-stage SMB analog moving bed system, is double-eluted by deionized water and 70-90 vol % of an ethanol aqueous solution, and rebaudioside C product liquid and rebaudioside F product liquid are collected from different outlets, respectively; and the rebaudioside F product liquid is concentrated and dried to obtain rebaudioside F powder. The method realizes the simultaneous separation of rebaudioside C and rebaudioside F.

The invention belongs to the technical field of agricultural biology, and particularly relates to application of a stevia rebaudiana low-phosphorus response gene PHR family in improving the content of glycoside in stevia rebaudiana. The invention provides application of a stevia rebaudiana low-phosphorus response gene PHR family in improving the content of glycoside in stevia rebaudiana, the stevia rebaudiana low-phosphorus response gene PHR family comprises a SrPHR1 gene and a SrPHR13 gene, and the relative content of rebaudioside C, steviobioside, rebaudioside D and total glycoside is improved by overexpressing the SrPHR1 gene; the relative content of stevioside and rebaudioside A is reduced through overexpression of the SrPHR1 gene; the relative contents of rebaudioside A, rebaudioside C and total glycosides are increased by overexpressing the SrPHR13 gene; the relative content of stevioside and rebaudioside D is reduced through overexpression of the SrPHR13 gene. The invention clarifies the influence of low phosphorus on the yield and quality of stevia rebaudiana, and preliminarily explores the application value of the phosphorus regulatory gene in improving the quality of stevia rebaudiana.