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Separation and purification method for rebaudioside C

A technology for separation and purification and stevioside, which is applied in the field of separation and purification of rebaudioside C, can solve the problems of little attention to rebaudioside C, and achieve the effects of low cost, high yield and high purity

Inactive Publication Date: 2018-03-09
苏州迪科传动机械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there are many separation and purification methods for rebaudioside A and stevioside, but little attention has been paid to rebaudioside C

Method used

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  • Separation and purification method for rebaudioside C
  • Separation and purification method for rebaudioside C

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Add 50kg of stevioside, a raw material containing 15.2% rebaudioside C, into a 500L reactor, then add 150L of deionized water and stir to raise the temperature to 70°C. After the solids are completely dissolved, remove the solution from the reactor and pumped into a single-effect concentrator to concentrate to about 50% of the original volume to obtain a sample.

[0023] (2) The above-mentioned sample is directly pumped into the top of the industrially prepared chromatographic column filled with a resinous polymer, and the weight of the resinous polymer is 10 times the weight of stevioside in step (1). Gradient elution with a mixed solution of water and acetone, the concentration gradient of the mixed solution of water and acetone is 10%-35% (volume ratio)

[0024] (3) Collect the elution solution eluted from the resin polymer chromatography column in sections, and then use high-performance liquid chromatography to analyze and detect it respectively. After the rebau...

Embodiment 2

[0030] Target raw material: 60kg of stevioside, purchased, including 24% stevioside, 27% rebaudioside A, 15.5% rebaudioside C, and the balance is impurities.

[0031] Chromatographic column: high-pressure industrial preparation column, column size 800*3000mm, filled with 1000L RohmHaasAmberlite1600.

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Abstract

The invention discloses a separation and purification method for rebaudioside C. A proper industrial chromatographic column and solution are adopted, a process step is optimized, and high performanceliquid chromatography detection is adopted and combines with column chromatography separation and recrystallization to obtain rebaudioside C from a stevioside raw material. The method is low in cost and high in purity and yield, is economic, and has the purity higher than 95% and yield higher than 60%.

Description

technical field [0001] The invention relates to a method for separating and purifying rebaudioside C. Background technique [0002] Stevioside has been used as a sweetener in Asia for more than 20 years. In 2008, it was approved as a food additive in North America. Therefore, steviol glycosides and their efficient purification methods are in high commercial demand. The main steviol glycosides in stevia include stevioside, rebaudioside C, rebaudioside A and rebaudioside D. Rebaudioside D and Rebaudioside C only account for a small part of it, but they have more pure sweetness and more unique applications, so they have higher commercial value. In the prior art, there are many separation and purification methods for rebaudioside A and stevioside, but little attention has been paid to rebaudioside C. [0003] Some studies have pointed out that although rebaudioside C has a strong bitter taste, it has many effects such as anti-tumor, lowering blood pressure, lowering blood fa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/256C07H1/06
CPCC07H1/06C07H15/256
Inventor 刘建
Owner 苏州迪科传动机械有限公司
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