56 results about "Steviolbioside" patented technology

The invention discloses a method for extracting stevioside from fresh stevia rebaudiana leaves, which comprises: crushing fresh stevia rebaudiana leaves with homogenizing crushing equipment under a condition of water, performing pressure filtration in continuous automatic discharge filtering equipment, subjecting the filtrate to flocculation by mixed flocculating agent; and absorbing by resin, analyzing, desalting and decolorizing by resin, concentrating and spray drying to obtain the stevioside product. In the invention, time and working procedures are saved, a long-distance transport process is avoided, raw material cost is lowered, discharge of waste water is reduced greatly, and clean production can be realized basically.

The invention discloses a natural compound sweetening agent and a preparation method thereof. The preparation method comprises the following steps of dissolving erythritol, a momordica grosvenori extract and stevioside into water; concentrating at temperature of 55 to 95 DEG C; crystallizing at pressure of -0.06 to -0.1MPa under the temperature of 50 to 70 DEG C; drying; and granulating to obtain the natural compound sweetening agent, wherein 97.8 to 99.9% of erythritol is added, and the sum of added momordica grosvenori extract and stevioside reaches 0.1 to 2.2% in mass percentage of raw materials. The sweetening agent prepared by the preparation method is high in purity, uniform in particle size and density, uniform in color and stabile in performance.

A process for producing the natural sweetener composition which comprises at least one of steviolbioside (STB) extract, Rebaudioside B extract and Rebaudioside D extract (“collectively, the “extracts”) comprises the steps of preparing a mother liquor comprising a mass content of at least 20% of at least one of the extracts; preparing feed liquid comprising at least 20 mg / mL of mother liquor; flowing feed liquid through a porous adsorption column, having a pore size of at least 40 Angstroms, a pore volume of a least 0.8 mL / g and at a flow rate of at least 1L / min and at a pH of between 4 to 5; eluting at least one steviolbioside (STB) extract, Rebaudioside B extract and Rebaudioside D extract with alcohol having a mass concentration of at least 65%; fractionally collecting one or more eluates based on chromatographic critical points for each of the steviolbioside STB extract, the Rebaudioside B extract and the Rebaudioside D extract; concentrating the extracts at a temperature of between 60-80° C.; and drying the extracts so formed.

The invention provides a method for purifying stevioside by using secondary ion exchange. Working procedures such as pretreatment, desalination, absorption, analyzing, decolorization, ion treatment, evaporation concentration, secondary ion exchange, steam aseptic filtration, spray drying and the like are carried out on stevia leaves so as to obtain stevioside products. Before spray drying, the ion exchange deep removal of impurity is carried out on the concentrated solution treated by the evaporation concentration, thus avoiding the possibility of the exchange removal of purity capability reduction of the ion exchange resin caused by the interference of organic solvent, causing the ion exchange resin to give full play to the function and advantage of the removal of impurity thereof, ensuring indicators of the product to reach the standard of the international top-grade products and the content of the product containing glucoside to reach more than 90% and the absorbance being less or equal to 0.04.

A method for purifying stevioside by recrystallization, the specific method is first heating and dissolving stevioside in 4-10 times the solvent under stirring, the heating temperature is ≤65°C, after it is completely dissolved, add cooling water to lower the temperature , lowered to 0°C-10°C for crystallization, the crystallization time is 12h-48h, the crystallization and mother liquor are separated by filtration, and then the crystals are crystallized again according to the crystallization conditions, and the purity of stevioside is higher than 95%. The invention is specially aimed at steviosides whose total glycosides are 65%-85%, and the method is simple and reasonable, easy to operate, and easy to form industrialized production.

The invention relates to the technical filed of seasonings, and in particular relates to a sausage seasoning with zanthoxylum schinifolium flavor and a making method of the seasoning. The seasoning comprises the following raw materials in parts by weight: 20-25 parts of hot pepper, 10-14 parts of zanthoxylum schinifolium, 1-8 parts of zanthoxylum bumgeanum, 3-5 parts of pepper, 3-5 parts of ginger, 6-8 parts of spices, 6-8 parts of fermented bean curd, 3-5 parts of fermented glutinous rice, 0.6-0.8 part of red kojic rice, 45-50 parts of edible salt, 20-25 parts of white wine, 5-7 parts of white granulated sugar, 5-8 parts of monosodium glutamate, 2-3 parts of edible glucose, 2-4 parts of starch, 0.08-0.1 part of monascus red, 0.08-0.1 part of stevioside and 0.5-0.8 part of D-sodium erythorbate. The cured product made by the seasoning provided by the invention has fragrant and tangy zanthoxylum schinifolium flavor, particular fragrance, lingering spicy aroma and unique fermentation flavor.

The invention discloses a purification method of steviolbioside (STB). The purification method comprises the following steps: preparing mother liquor saccharide into 25-40mg / ml feed liquid; leading the feed liquid to flow over a macropore absorbent resin column at the speed of 1.0-2.0L / min, wherein the resin column is styrene type polar co-polymer, the average pore size of the resin column is 40-50 A, the pore volume of the resin column is 0.9-1.0ml / g, and a pH value during the absorption is 4.0-5.0; and after the absorption is completed, eluting out STB absorbed on the resin column with 67-72% ethanol, collecting eluent section by section, defining a critical point, collecting the eluent at the critical point, concentrating the eluent at the temperature of 60-80 DEG C, and respectively drying the obtained solid and liquid so as to obtain crude STB. The purity of the STB prepared by the purification method is high, and the content of the STB can reach above 40%; and the content of STB in the stevioside reaches above 95% by rectification.

The invention discloses a mechanical continuous extraction process for stevioside and a continuous extraction unit. The continuous extraction process comprises the following steps of: 1, saturated imbibition immersion; 2, carrying out mechanical continuous extraction by adopting the continuous extraction unit; 3, post-recycling treatment; and 4, follow-up treatment. The continuous extraction unitcomprises a shell, a high-voltage kneading and tearing device and a low-voltage expanding imbibition device which are arranged inside the shell; both the high-voltage kneading and tearing device and the low-voltage expanding imbibition device comprise horizontal thrusters; the horizontal thrusters are of twin-screw extruders and comprise propelling main shafts and propelling countershafts which are synchronous in the rotation and opposite in the rotation direction; and helical blades, which are opposite in the helical direction, are arranged on the propelling main shafts and the propelling countershafts. The process and the continuous extraction unit have the advantages of reasonable design, convenience for implementation, energy saving, environment friendliness, little water consumption,high production efficiency, high extraction efficiency and capability of effectively solving the practical problems, such as larger water consumption, great waste water handling capacity, high investment cost, low production efficiency, low extraction rate and the like when the traditional pot-type steeping tank is used for extraction.

The invention provides a method for purifying stevioside RC crude extracts. Mother liquor sugar is prepared into 15 to 20mg / ml feed liquor, the feed liquor flows through large-pore resin absorption columns at the flow speed being 1.5 to 3.0L / min, the large-pore resin absorption columns are benzethylene type nonpolar copolymers and have the average pore size being 5 to 6nm and the pore volume being 1.2 to 1.4 ml / g, and the pH value during the adsorption is 4.0 to 5.0; after the adsorption is completed, ethanol with mass concentration being 75 percent to 80 percent is used for eluting the stevioside absorbed on the resin columns, the elution liquor is collected in a sectional way, a critical point is determined, the elution liquor at the critical point is concentrated at the temperature of 60 to 80 DEG C, obtained solid and liquid are respectively dried, and the crude stevioside is obtained. The stevioside prepared by the method has higher purity, and in addition, the RC content can reach more than 30 percent. The method can adapt to the diversified market requirements of consumers.

The invention provides a solid alcohol solid dispersion combination of EGCG (epigallocatechin gallate) with thermal stability and sugar alcohols. The sugar alcohols include one or more ingredients comprising fructose, newtol, fructo-oligose, xylooligosaccharide, polydextrose, mannitol, cane sugar, glucose, resistant dextrin, sorbitol, maltose, isomalt, mannose oligomer, carbo wax, dextrin, cyclodextrin, hydroxyl-lpropyl-beta-cyclodextrin, microcrystalline cellulose, carboxymethylcellulose, pregelatinized starch, starch, starch sugar, lactose, sodium carboxymethyl starch, proteoglycan, sucralose, aspartame, acesulfame, stachyose, neotame and stevioside, and the sugar alcohols and the EGCG are in (12-80):1 in proportion by weigth. The invention further provides a preparation method of the solid dispersion combination and application in preparation of the solid dispersion combination. By the aid of the solid dispersion combination, the EGCG is effectively improved in stability.

The present invention relates to a chewing gum with the functions of reducing fat and weight and its production method. Its composition includes the Chinese medicine preparation made up by using (by weight portion) 10-25 portions of crataegus fruit, 10-18 portions of fetid cassia, 5-10 portions of astragalus root and 8-12 portions of salvia root, stevioside, gum base, perfume, hydrogenated oil and starch, and its production method includes the following steps: preparing the Chinese medicine powder preparation and auxiliary material, mixing the Chinese medicine powder and starch, puffing and cooling to room temp., adding prepared gum base, adding other axuliary material, uniformly stirring them to obtain chewing gum blank, pressing to form so as to obtain the invented product. Besides, it also possesses the functions of fortifying spleen and supplementing kidney. Its production process is simple and cost is low.

The invention relates to the technical field of bamboo wood mould proofing and corrosion proofing, in particular to an outdoor bamboo wood natural mould-proof corrosion-proof agent and preparation andtreatment methods thereof. The bamboo wood natural mould-proof corrosion-proof agent is prepared from the following components in percentage by weight: 30-40% of industrial wastewater after stevia rebaudiana is processed to prepare stevioside, 5-10% of soapberry fruit, 20-30% of melia azedarach leaf, 10-15% of folium coptis chinensis and 10-20% of folium isatidis. According to the preparation method of the bamboo wood natural mould-proof corrosion-proof agent, conventional decoction or alcohol extraction is adopted; the method for treating bamboo wood by means of the bamboo wood natural mould-proof corrosion-proof agent comprises the steps that after the bamboo wood is placed in hot water for soaking treatment or placed in an ethyl alcohol solution for soaking treatment, the bamboo wood natural mould-proof corrosion-proof agent is used for treatment. The outdoor bamboo wood natural mould-proof corrosion-proof agent and the preparation and treatment methods thereof have the beneficialeffects that firstly, the bamboo wood is placed in the alcohol solution for soaking to make large amounts of starch, sugar, protein and other salt substances in bamboo wood tissues are extracted through alcohol; therefore, the condition that the natural organic mould-proof corrosion-proof agent penetrates through parenchyma cells and vascular bundles of the internal tissues of the bamboo wood is more easy; the mould-proof corrosion-proof agent is prepared from the natural organic extracts and do not have any toxicity or cause pollution problems to the environment.

The invention provides dendrobium officinale granules, relates to nutritional products, and particularly relates to dendrobium officinale granules which use dendrobium officinale as a main material and is good in administration taste. The dendrobium officinale granules are characterized by comprising dendrobium officinale, American ginseng, astragalus membranaceus, lactose, dextrin and stevioside. The granules are prepared in the following steps: preparing extract; preparing fine powder; mixing; preparing a soft material; granulating; and drying, packaging and the like. According to the dendrobium officinale granules, the traditional Chinese medicinal materials are subjected to extraction, baking, crushing, screening and other detailed indicator quantity control in the processing process, so that the problem that a product user refuses to eat dendrobium officinale due to resistance to drugs can be solved, the dendrobium officinale granules have the characteristics of long storage time, higher drug effect, high bioavailability and the like, can be well absorbed by a human body, and are excellent in administration effect.

The invention discloses a method for conversing steviolbioside to rebaudiodside B, which belongs to the food chemical field, steviolbioside is taken as a raw material, cane sugar is taken as a carbohydrate source, so that the rebaudiodside B can be conversed under the catalysis of aspergillns niger III. According to the invention, the conversion rate of the rebaudiodside B which is obtained by taking steviolbioside as the raw material is greater than or equal to 70%, the rebaudiodside B content in a solid content can reach at least 60%, and can reach 80% after further separation and purification, and even can reach greater than 90%, the rebaudiodside B tastes odorless, and can be individually used as a sweetener, the rebaudiodside B produced by the method has the advantages of low cost and easy amplification, and is suitable for industrial production.

The present invention provides a Eucommia ulmoides olive fungal substance sugar-free food. The food comprises the following raw materials in percentage by weight: Eucommia ulmoides olive fungal substance 10-20%, pure pumpkin powder 15-50%, wheat standard flour 30-50%, edible vegetable oil 4-8%, shortening 4-8%, baking powder 0.1-0.5%, Steviosides 0.02-0.06%, and salt 0.1-0.2%. The preparation method comprises the following steps: crushing raw material Eucommia ulmoides olive, weighting, blending, bottling and sterilizing to obtain Eucommia ulmoides olive culture medium according to a certain proportion; inoculating the culture medium with test-tube mother culture of edible fungi or medicinal fungi, and fermenting to obtain Eucommia ulmoides olive fungal substance, adding the pure pumpkin powder and the wheat standard flour into the Eucommia ulmoides olive fungal substance and blending, subjecting the mixture to baking or freezing process to produce the fungal substance sugar-free food. The food does not contain sugar type components with high calorific value, has the special flavor of fungal substance and is suitable for regular consumption of patients with diabetes and cardio-cerebral diseases such as hypertension, hyperlipidemia and high thrombosis.

The invention discloses an lsine hydrochloride and zinc gluconate composite particle and a preparation method thereof. The lsine hydrochloride and zinc gluconate composite particle comprises the following components in parts by weight: 10-15 parts of lysine hydrochloride, 3-4 parts of zinc gluconate, 0.5-2 parts of sweetening agent, 1.0-2.5 parts of citric acid, 60-90 parts of filling agent, 3-5 parts of povidone K30 and 0.5-1.0 part of essence. The invention also discloses a sugar-free type lysine and zinc gluconate particle, the sugar-free type lysine and zinc gluconate particle / 10000 g comprises the following components by weight: 125 g of lysine hydrochloride, 35 g of zinc gluconate, 12.5 g of citric acid, 5 g of stevioside and 822.5 g of dextrin. The sugar-free type lysine and zinc gluconate particle disclosed by the invention is particularly suitable for diabetics.

Methods of preparing highly purified steviol glycosides, particularly steviolmonoside, steviolmonoside A, steviolbioside, steviolbioside D, rubusoside, steviolbioside A, steviolbioside B, rebaudiosideB, stevioside, rebaudioside G, stevioside A, stevioside B, stevioside C, rebaudioside A, rebaudioside E, rebaudioside E2, rebaudioside E4, rebaudioside E6, rebaudioside E3, rebaudioside D, rebaudioside I, rebaudioside AM, rebaudioside D7, rebaudioside M, rebaudioside M4, rebaudioside 1a, rebaudioside 1b, rebaudioside 1c, rebaudioside 1d, rebaudioside 1e, rebaudioside 1f, rebaudioside 1g, rebaudioside 1h, rebaudioside 1i, rebaudioside 1j, rebaudioside 1k, rebaudioside 1l, rebaudioside 1m, rebaudioside 1n, rebaudioside 1o, rebaudioside 1p, rebaudioside 1q, rebaudioside 1r, rebaudioside 1s, rebaudioside 1t, rebaudioside 2a, rebaudioside 2b, rebaudioside 2c, rebaudioside 2d, rebaudioside 2e, rebaudioside 2f rebaudioside 2g, rebaudioside 2h, rebaudioside 2i, rebaudioside 2j, rebaudioside 2k, rebaudioside 21, rebaudioside 2m, rebaudioside 2n, rebaudioside 2o, rebaudioside 2p, rebaudioside 2q, rebaudioside 2r, rebaudioside 2s and / or SvG7 are described. The methods include utilizing enzyme preparations and recombinant microorganisms for converting various staring compositions to target steviol glycosides. Highly purified steviol glycosides are useful as non-caloric sweeteners, flavor enhancers, sweetness enhancers and foam inhibitors in edible and chewable compositions such as any beverage, confectionery, bakery, biscuits and chewing gums.

The invention provides a method for extracting stevioside from whole-plant stevia. The method comprises the following steps of: under the water adding condition, crushing the whole-plant stevia with a grinder; filtering with continuous automatic discharging filtration equipment; flocculating a filtrate with a flocculant; and carrying out resin adsorption, desorption, resin desalting and decoloring, concentration and spray drying processes so as to prepare the stevioside product. By using the method, time and processes are saved, long-distance transport links are reduced, raw material cost is reduced, the external emission of wastewater is greatly reduced, and clean production is basically achieved.

The invention discloses a fruit cake capable of strengthening the spleen and nourishing the stomach, which belongs to the technical field of food processing and is prepared from the following raw materials in parts by weight: 50-60 parts of fruits, 2-7 parts of pectin, 3-6 parts of edible gelatin, 4-8 parts of stevioside, 2-5 parts of malt syrup, 1-2 parts of organic acid, 0.2-0.4 part of papain, 0.05-0.2 part of tomatin, 0.1-0.4 part of lactobacillus casei and 90-130 parts of water. The fruit cake obtained through combined action of all the components is bright in color, fine and smooth in taste, rich in elasticity, rich in nutrient components, simple in preparation method, convenient in technological operation and suitable for large-scale production and application.

The invention discloses an extraction separation method of salvianolic acid B. The method comprises the following steps of (1) radix salviae miltiorrhizae medical material selection: selecting radix salviae miltiorrhizae medical material decoration pieces conforming to the specification of pharmacopeia; (2) extraction: weighing 1 part of radix salviae miltiorrhizae medical material decoration pieces in parts by weight; adding 12 parts of water with the pH being 5.0; adding xylanase, rebaudioside and / or stevioside accounting for 5 to15 U in per g of medical materials; performing reaction for 2to 3 hours at 38 to 45 DEG C; performing cooling and filtering to obtain filter liquid; (3) purification: performing vacuum concentration on the extraction liquid to 1 / 5 original volume; performing purification by macroreticular adsorption resin. The xylanase, rebaudioside and stevioside are comprehensive used, so that the enzymic method processing time can be effectively reduced; in the purification process, the activity of the salvianolic acid B can also be protected, so that the stability of the salvianolic acid B in the purification process can be greatly improved. When the two ingredientsare singly used, the effect can be respectively achieved; after the combination, the cooperated effects are achieved.

The invention provides a solid dispersion composition of levocarnitine and a preparation method of the solid dispersion composition. Sugar alcohol substances include one or more of fructose, xylitol, polydextrose, fructo-oligose, mannitol, cane sugar, glucose, resistant dextrin, sorbitol, maltose, isomaltulose alcohol, manoligosaccharides, dextrin, cyclodextrin, hydroxypropyl-beta-cyclodextrin, microcrystalline cellulose, carboxymethylcellulose, hydroxypropyl methylcellulose, pre-gelled starch, starch, starch sugar, lactose, sodium carboxymethyl starch, protein sugar, sucralose, aspartame, acesulfame potassium, stachyose, Neotame and stevioside; the sugar alcohol substances are combined with the levocarnitine in a weight ratio of (13-50):1; the levocarnitine further comprises levocarnitine tartrate or levocarnitine hydrochloride. The solid dispersion composition has excellent moisture absorption resisting quality stability and is significant for the improvement of the product quality.

The invention relates to the technical field of healthy food, and particularly discloses a compound anti-aging composition, a compound anti-aging tablet and a preparation method and application of the compound anti-aging composition and the compound anti-aging tablet. The compound anti-aging compositio is prepared from 100 to 200 parts of NMN, 2 to 3 parts of PQQ, 5 to 15 parts of quercetin, 200 to 400 parts of betaine and 300 to 400 parts of auxiliary materials, wherein the auxiliary materials comprise one or more of stevioside, calcium phosphate, magnesium stearate and silicon dioxide. According to the compound anti-aging compositio disclosed by the invention, the betaine is matched with the PQQ and the NMN, so that the cell osmotic pressure can be effectively regulated, the intestinal tract is protected, the stress reaction of the compound anti-aging compositio is reduced, and fat metabolism and protein synthesis of a human body are promoted; the effects of promoting energy metabolism of human cells and accelerating metabolism are also achieved while the immunity of the organism is enhanced; and the betaine can effectively reduce the probability that NMN, PQQ and quercetin are oxidized, so that the anti-oxidation, anti-inflammation and anti-aging effects of quercetin, PQQ and NMN on human cells after the quercetin, PQQ and NMN are compounded are further stimulated.

The present invention provides a low sugar five kernel and roast pork moon cake and a preparation method thereof. The moon cake comprises the following raw materials in parts by weight: 2-6 parts of stevioside, 3-6 parts of synsepalum dulcificum fruits, 0.5-1 part of alkaline water, 4-8 parts of flour, 2-6 parts of peanut oil, 2-5 parts of peanut kernels, 2-5 parts of walnut kernels, 2-5 parts of white sesame seeds, 2-5 parts of shelled melon seeds, 2-5 parts of apricot kernels, 2-5 parts of glutinous rice flour, 4-8 parts of roast pork and 2-5 parts of fructus swietenia macrophylla. The raw materials are weighed according to the parts by weight, stuffing materials and skin materials are prepared, and shaping and baking are conducted to obtain the low sugar five kernel and roast pork moon cake. The prepared moon cake is low in sugar content and suitable for patients with diabetes to feel relieved to eat. Moon cake skins are loose in mouthfeel and do not deform. The fructus swietenia macrophylla, peanut kernels, walnut kernels, white sesame seeds, shelled melon seeds, apricot kernels and roast pork are added into the stuffing materials. The moon cake is rich in nutrition elements, can enhance human immunity, and effectively promotes blood circulation.

The invention provides compound sustained release particle tea and a preparation process thereof. The tea is prepared from, by mass, 30-60 parts of lucid ganoderma superfine powder, 30-60 parts of tea leaf superfine powder, 5-20 parts of soluble coffee, 2-5 parts of chrysanthemum superfine powder, 20-50 parts of microcrystalline cellulose, 10 parts of soluble starch, 0.1-1 part of sodium carboxymethylcellulose, 0.5-1.2 parts of beta-cyclodextrin and 0.04-0.08 part of stevioside. The preparation process mainly utilizes the superfine smashing technology and the granulation technology. When processed through the superfine smashing technology and the granulation technology, the product has excellent taste, color and luster and functions of improving body immunity, tranquilizing and allaying excitement and the like, and is simple in preparation process and convenient to operate.

The invention relates to the technical field of dosage forms of health care products, in particular to an oral micro-effervescent tablet and a preparation method thereof. The oral micro-effervescent tablet includes nutrients, sugar alcohol, acid source, alkali source, magnesium stearate, essence and sweetener; the sugar alcohol is lactose, maltitol, sorbitol, isomalt, mannitol , erythritol, or a combination of several; the sweetener is one or a combination of sucralose, aspartame, stevioside, fructose, white sugar, and mogroside. In the present invention, by adding appropriate amount of acid-base, sweetener and essence into the nutritional substances, under the action of saliva in the oral cavity, the nutrient substance will effervesce slightly, with good taste and strong sense of experience. At the same time, the product of the invention solves the problem of serious moisture absorption of the effervescent tablet in the production process or accelerated test.

The invention relates to a method for producing cellulose by virtue of fermentation and specifically relates to a method for increasing yield of cellulose by adding stevia rebaudiana sugar glucoside. The stevia rebaudiana sugar glucoside is taken as a cellulose fermentation inducer for inducing trichoderma to produce the cellulose. The method for inducing the trichoderma to produce the cellulose by taking the major component stevioside of the stevia rebaudiana sugar glucoside as the cellulose fermentation inducer is wide in raw material source, low in cost and convenient for large-scale production and application.

Methods of preparing highly purified steviol glycosides, particularly steviolmonoside, steviolmonoside A, steviolbioside A, steviolbioside B, steviolbioside C, steviolbioside D, steviolbioside E, rubusoside, dulcoside A, dulcoside C, dulcoside D, stevioside A, stevioside B, stevioside C, stevioside G, stevioside H rebaudioside B2, rebaudioside A4, rebaudioside C, rebaudioside C3, rebaudioside C4,rebaudioside C5, rebaudioside C6, rebaudioside E3, rebaudioside E4, rebaudioside E5, rebaudioside E6, rebaudioside E7, rebaudioside D5, rebaudioside D6, rebaudioside D7, rebaudioside D8, rebaudiosideH2, rebaudioside H3, rebaudioside H4, rebaudioside H5, rebaudioside H6, rebaudioside K, rebaudioside N2, rebaudioside N3, rebaudioside N4, rebaudioside N5, rebaudioside M3 and / or rebaudioside 04 are described. The methods include utilizing enzyme preparations and recombinant microorganisms for converting various staring compositions to target steviol glycosides. The highly purified steviol glycosides are useful as non-caloric sweetener, flavor enhancer, sweetness enhancer, and foaming suppressor in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.

The invention discloses a method for refining and purifying steviolbioside, and belongs to the technical field of natural extract refining. The method comprises the following steps: adding water to dissolve stevia rebaudiana to obtain a steviolbioside containing solution, supplementing H<+> into the solution for crystallization, and performing solid-liquid separation to obtain a crystallization product, wherein the crystallization product is a refined steviolbioside product. The method is simple in process and controllable in condition, and a stevioside product with steviolbioside content of 85 percent or higher can be obtained.

Methods of preparing highly purified steviol glycosides, particularly steviolmonoside, steviolmonoside A, steviolbioside, steviolbioside D, rubusoside, steviolbioside A, steviolbioside B, rebaudioside B, stevioside, rebaudioside G, stevioside A, stevioside B, stevioside C, rebaudioside A, rebaudioside E, rebaudioside E2, rebaudioside E4, rebaudioside E6, rebaudioside E3, rebaudioside D, rebaudioside 1, rebaudioside AM, rebaudioside D7, rebaudioside M, rebaudioside M4, rebaudioside 1a, rebaudioside 1b, rebaudioside 1c, rebaudioside 1d, rebaudioside 1e, rebaudioside 1f rebaudioside 1g, rebaudioside 1h, rebaudioside 1i, rebaudioside 1j, rebaudioside 1k, rebaudioside 1l, rebaudioside 1m, rebaudioside 1n, rebaudioside 2a and / or SvG7 are described. The methods include utilizing enzyme preparations and recombinant microorganisms for converting various staring compositions to target steviol glycosides. The highly purified steviol glycosides are useful as non-caloric sweetener, flavor enhancer, sweetness enhancer, and foaming suppressor in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.