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Purification method of steviolbioside (STB)

A technology of stevioside and purification method, which is applied in the field of purification of stevioside STB, and can solve problems such as no STB purification method

Active Publication Date: 2011-06-15
安徽润海生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Currently, the steviol glycoside products on the market are mainly RA and STV, and there is no STB-based product. Therefore, the extraction method of stevioside mainly focuses on the purification and refining of RA and STV, and there is no better purification of STB. method

Method used

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  • Purification method of steviolbioside (STB)
  • Purification method of steviolbioside (STB)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Take mother liquid sugar, analyze the content of STB by liquid chromatography to be 23.74%, make the mother liquid sugar into a feed liquid with a concentration of 30mg / ml, take 1400L of the feed liquid, and use a slow flow rate of 1.5L / min Flow through the 500L DA-201-L resin column produced by Jiangsu Suqing Water Treatment Company. During the process of the material liquid flowing through the resin column, the resin column selectively adsorbs the material liquid according to the polarity of different components of steviol glycosides. The pH of the adsorption environment is 5.5. After 15 hours, the adsorption of the material solution is completed, and the steviol glycosides adsorbed on the resin are analyzed with 1400L of 70% ethanol. The eluent was cut in sections with 100L as the unit, and the content of STB was detected by liquid chromatography analysis. It was found that steviol glycosides RB began to be eluted in large quantities at 800L of the eluent. ...

Embodiment 2

[0036] Example 2: Take 20 kg of crude steviol glycosides with 48.86% of STB prepared in Example 1 and 30 kg of ethanol with a concentration of 88% and 37% methanol in a 1:3 ratio. After the dissolution is completed at 65℃, the temperature will drop to room temperature rapidly within 5 minutes. The mixture is stirred for 3 minutes every 4 hours. After leaving for 48 hours, the dissolved mixture is separated into solid and liquid, and the filtered solid is added with no brine to adjust the concentration. Concentrate to 27% and concentrate to 43%. The solution is dried to obtain 6.2 kg of refined steviol glycosides. The content of steviol glycosides in the refined stevioside is 95.73%; the liquid obtained by solid-liquid separation is evaporated to remove ethanol and excess water, The concentration of the aqueous solution of steviol glycosides was adjusted to 47%, and 11 kg of refined steviol glycosides were obtained after drying. The total recovery rate of steviol glycosides was 8...

Embodiment 3

[0037] Example 3: A mixed solvent of 20 kg of crude steviol glycosides with 48.86% of STB content prepared in Example 1 mixed with 32 kg of 90% ethanol and 35% methanol in a ratio of 1:3 After the dissolution is completed at 67℃, the temperature will drop to room temperature rapidly within 7 minutes, and the mixture will be stirred for 5 minutes every 5 hours. After standing for 60 hours, the dissolved mixture will undergo solid-liquid separation through a plate and frame filter press, and the filtered solid Add no salt water to adjust the concentration to 25%, concentrate to 45%, and dry the solution to obtain 6.4 kg of refined stevioside. The content of stevioside STB in the refined stevioside is 95.34%; the liquid obtained by solid-liquid separation is evaporated. The concentration of the aqueous solution of steviol glycosides was adjusted to 45% with ethanol and excess water, and 11.5 kg of refined steviol glycosides were obtained after drying. The total recovery rate of ste...

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Abstract

The invention discloses a purification method of steviolbioside (STB). The purification method comprises the following steps: preparing mother liquor saccharide into 25-40mg / ml feed liquid; leading the feed liquid to flow over a macropore absorbent resin column at the speed of 1.0-2.0L / min, wherein the resin column is styrene type polar co-polymer, the average pore size of the resin column is 40-50 A, the pore volume of the resin column is 0.9-1.0ml / g, and a pH value during the absorption is 4.0-5.0; and after the absorption is completed, eluting out STB absorbed on the resin column with 67-72% ethanol, collecting eluent section by section, defining a critical point, collecting the eluent at the critical point, concentrating the eluent at the temperature of 60-80 DEG C, and respectively drying the obtained solid and liquid so as to obtain crude STB. The purity of the STB prepared by the purification method is high, and the content of the STB can reach above 40%; and the content of STB in the stevioside reaches above 95% by rectification.

Description

Technical field [0001] The invention relates to a method for extracting steviol glycosides, in particular to a method for purifying steviol glycosides STB. Background technique [0002] After being refined, stevia is widely used in food, beverage, wine making, medicine, cosmetics and other fields. In recent years, stevioside, an extract of stevia, has been used as a sweetener. It has very low caloric energy and will not cause adverse reactions to patients with dental diseases and diabetes. It is an excellent sweetener and has a broad market prospect. Steviol glycosides are the general term for the extracts of Stevia rebaudiana. At present, in the official method of the US FDA, the steviol glycosides mainly involve the following 9 components: Stevioside (Stevioside), Rebaudioside A (Labaudioside A), Rubusoside, Dulcoside A, Rebaudioside C, Rebaudioside F, Rebaudioside D, Steviolbioside Glycoside), Rebaudioside B (Rebaudioside B), Steviol glycoside STB is one of the components S...

Claims

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Application Information

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IPC IPC(8): C07H15/24
Inventor 张永李存彪陈彩霞
Owner 安徽润海生物科技股份有限公司
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