380 results about "Steviol glycoside" patented technology

The present disclosure provides enzymes and methods of using those enzymes to transfer sugar moieties to the substrate steviol glycosides. The designed beta-1, 3-glycosyltransferase can be used for adding a glucose monomer connected with beta-1, 3 to steviol glycosides. In particular, the designed beta-1, 3-glycosyltransferase is used in a one-pot reaction together with a beta-1, 2-glycosyltransferase and a sucrose synthase to convert stevioside and / or Reb A to Reb M.

Compared with the prior art, the traditional Chinese medicine compound oral liquid is mainly used for invigorating qi and blood, regulating yin and yang, nourishing liver and kidney, strengthening spleen and stomach, strengthening muscles and bones and the like, a plurality of traditional Chinese medicine components are compounded and decocted to obtain liquid medicine, and finally specific modified polysaccharide, steviosin, sodium benzoate and ethylparaben are added, so that the traditional Chinese medicine compound oral liquid is prepared. The modified polysaccharide is screened and optimized and is added into the compound oral liquid, and the problems of serious wall hanging and high liquid medicine waste rate in the prior art are solved by reducing the low-temperature storage wall hanging rate and the hydrolysis rate in storage stability on the premise of not influencing the medicine effect and safety.

The invention relates to the technical field of image data processing, in particular to a stevioside impurity detection method based on industrial vision, which comprises the following steps: placing a supersaturated solution in a crystallization container for cooling crystallization to obtain surface images of each detection area under different illumination angles so as to determine a gray level fluctuation coefficient of each detection area; screening a feature detection area, acquiring a surface image of the feature detection area at a feature acquisition illumination angle to identify a feature contour, acquiring feature contours at different crystallization temperatures, determining a contour growth coefficient, determining a risk tendency category of the feature contour, and determining a mode of performing impurity marking; according to the method, a high-risk region where impurities exist is rapidly identified, the impurity marking mode is adaptively adjusted according to different crystal contour features in the surface image of the stevioside crystal, and the stevioside impurity detection reliability and detection efficiency are improved.

The present disclosure relates to a composition for producing steviol glycosides containing Rebaudioside M at high purity, using a biocatalyst containing UDP-glycosyltransferase and UGT76G1, and a method for producing steviol glycosides using the same.

The invention discloses a method for improving phosphorus utilization of lateolabrax japonicus and application of stevioside of the lateolabrax japonicus. The phosphorus utilization of lateolabrax japonicus is improved by adjusting a PI3K / AKT pathway. The method is simple to operate, and raw materials are easy to obtain, safe and harmless. Meanwhile, a PI3K / AKT pathway can be effectively activated, insulin resistance and lipid metabolism of lateolabrax japonicus are improved, so that the phosphorus utilization rate of lateolabrax japonicus is increased, and the growth performance of lateolabrax japonicus is improved.

The invention belongs to the technical field of biosynthesis, and particularly relates to a method for regulating and controlling stevioside production through a UDPG synthesis route. According to the invention, a high-efficiency stevioside biosynthesis system is constructed through systematic engineering modification: in the aspect of chassis cell optimization, transcription factors (AtWRKY18, AtWRKY40 and AtMYC2) for regulating and controlling MEP pathway and key genes (dxs and / or dxr) of the MEP pathway are over-expressed, so that supply of terpene precursors is enhanced; and a heterologous MVA pathway (over-expression T7-atoB-mvaS-mvaE-mvK / mvKmut-pmK-mvaD-idi) with a modular design is introduced, so that sufficient supply of the terpenoid precursor is realized. Meanwhile, the intracellular concentration of the UDPG is increased by systematically enhancing a UDPG synthesis pathway (knocking out ugd, glgc, ushA, agp, deoA, tdk, udp, or thrA, and overexpressing galU, pgm, pyrE, pyrH, ugpA, or prs), so that the production capacity of the stevioside is remarkably improved.

The invention discloses a high-power compound plant sweetener and a preparation method thereof, and belongs to the technical field of food chemistry. Every 100 g of the high-power compound plant sweetening agent is prepared from the following components: 0.001 to 5 g of mogroside, 0.001 to 2 g of stevioside, 0.001 to 5 g of liquorice root extract, 0.0001 to 2 g of neohesperidin dihydrochalcone, 0.01 to 0.015 g of stachyose, 0.005 to 0.01 g of D-erythorbic acid, 0.005 to 0.01 g of ascorbic acid, and the balance of water-soluble dietary fiber. The pure plant high-power sweetener which is safe and healthy, has low energy and low carbohydrate and is rich in dietary fiber and protein is prepared, has obvious functions of healthy food, and is low in energy and less in carbohydrate and contains a certain amount of protein compared with cane sugar under the same condition.

The invention belongs to the technical field of biosynthesis, and particularly relates to a method for regulating and controlling stevioside production by improving the utilization rate of a carbon source. According to the invention, a high-efficiency stevioside biosynthesis system is constructed through systematic engineering modification: in the aspect of chassis cell optimization, transcription factors (AtWRKY18, AtWRKY40 and AtMYC2) for regulating and controlling MEP pathway and key genes (dxs and / or dxr) of the MEP pathway are over-expressed, so that supply of terpene precursors is enhanced; and a heterologous MVA pathway (over-expression T7-atoB-mvaS-mvaE-mvK / mvKmut-pmK-mvaD-idi) with a modular design is introduced, so that sufficient supply of the terpenoid precursor is realized. Meanwhile, by knocking out related genes and bypass metabolism genes (mgsA, iclR, menA or adhE) synthesized by organic acid, a glucose transport and utilization system (fnr, fadR, ppc, zwf or pgi is knocked out, and ACS, fbaA, tpiA, glf, galP or ptsG is overexpressed, so that the utilization rate of a carbon source is further improved, and the production capacity of stevioside is remarkably improved.

The invention belongs to the technical field of stevioside production, and discloses a preparation method of glucosyl stevioside, which comprises the following steps: adding enzyme into stevioside and a glycosyl donor in a reaction system to prepare the glucosyl stevioside; inactivating after the reaction is completed, and cooling to room temperature to obtain a conversion solution; the method comprises the following steps: adding 0.01% by volume of hydrolase for hydrolysis, separating through an ultrafiltration membrane to obtain membrane trapped fluid, concentrating and drying the membrane trapped fluid, adding ethanol for redissolving, adding diatomite for adsorbing impurities, filtering, concentrating and dealcoholizing the obtained clear liquid, and drying to obtain the glucosyl stevioside product. According to the product disclosed by the invention, the production of glucosyl stevioside total glycosides is improved, the proportion of the whole glycosylation chain length is regulated and controlled, the sweetness and sweetness of the mouth feel are improved, the solubility of the glucosyl stevioside total glycosides in application is also increased, the problem of clarification of the glucosyl stevioside total glycosides in a high-alcohol state is solved, and the market application range is expanded.

The invention relates to a stevioside composition, which comprises at least one stevioside and at least one of siamenoside I, mogroside V, mogroside III-E and mogroside II-E, and is characterized in that the stevioside composition comprises at least one stevioside and at least one of siamenoside I, mogroside V, mogroside III-E and mogroside II-E; wherein the stevioside has a higher solubility in the composition than in an equivalent composition lacking siamenoside I, mogroside V, mogroside III-E, and mogroside II-E. The invention also relates to a method for preparing the stevioside composition. The composition may be an aqueous solution.

The invention relates to an engineered glycosyltransferase and a steviol glycoside glucosylation method. The present invention provides engineered glycosyltransferases (GTs), polypeptides having GT activity and polynucleotides encoding the enzymes, as well as vectors and host cells comprising the polynucleotides and polypeptides. The present invention provides engineered sucrose synthases (SuS), polypeptides having SuS activity and polynucleotides encoding the enzymes, as well as vectors and host cells comprising the polynucleotides and polypeptides. The invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to prepare products having beta-glucose ligation. The present invention also provides compositions and methods for producing rebaudioside (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The invention also provides compositions comprising the SuS enzymes and methods of using the same. Methods for producing a GT enzyme and a SuS enzyme are also provided.

The present disclosure provides a combination of steviol glycosides having various flavor characteristics. In one aspect, the present disclosure provides a rebaudioside composition comprising at least one of rebaudioside A, rebaudioside D, and rebaudioside M. The present disclosure provides a rebaudioside composition comprising at least one of rebaudioside A, rebaudioside D, and rebaudioside M. In one embodiment, the present disclosure provides a rebaudioside composition comprising rebaudioside A, rebaudioside D, and rebaudioside M. In one embodiment, the present disclosure provides a rebaudioside composition comprising rebaudioside A, rebaudioside D, and rebaudioside M. The content of rebaudioside A, rebaudioside D, and / or rebaudioside M in the rebaudioside composition of the present disclosure may be about 5%, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 95%, etc.

The present disclosure provides enzymes and a method to use those enzymes to transfer a sugar moiety to a substrate steviol glycoside. Specifically, designed beta-1,2-glycosyltransferases and sucrose synthases are used in a one-pot reaction to convert stevioside and Reb A into Reb E and Reb D. Additionally, the designed beta-1,2-glycosyltransferases can be used in a one-pot reaction with a sucrose synthase and a beta-1,3-glycosyltransferase to produce Reb M from stevioside and / or Reb A.

The invention belongs to the technical field of functional food, and particularly relates to a compound fermentation composition for regulating immunity and intestinal microecology as well as a preparation method and application of the compound fermentation composition. The invention provides a compound fermentation composition for regulating immunity and intestinal microecology. Comprising the following components: blueberry freeze-dried powder, an astragalus extract, a lycium ruthenicum extract, a day lily flower extract, grifola frondosa polysaccharide, schizochytrium limacinum powder, a red yeast rice extract, polygonatum polysaccharide, cordyceps militaris, xylooligosaccharide, yeast beta-glucan, lactoferrin, stevioside, a complex microbial inoculant and a complex enzyme. According to the compound fermentation composition disclosed by the invention, high-efficiency immunoregulation, long-acting intestinal balance and maximum component utilization are realized, and an efficient, stable and applicable solution is provided for regulating immunity and intestinal microecology.

The present invention relates to a glycosyltransferase mutant, and a composition and a method for producing steviol glycosides using the same, and more specifically, to a glycosyltransferase of rebaudioside that transfers glucose to steviol glycosides and its mutant, a recombinant strain expressing the enzymes, and a method for producing rebaudioside using the same.

The disclosure in one aspect provides a pharmaceutical composition of an antiviral agent, the pharmaceutical composition comprising: the antiviral agent, which can be tenofovir; and a PEG-saccharide-lipid conjugate having the structural formula wherein m has a number-average value in the range of 2-10; S is a mono-, di- or trisaccharide group; L is -C(O)-R1 in which R1 is an alkanyl or alkenyl group having a number- average number of carbons in the range of 6-22, and / or is a steroid acyl group; P is -(CH2-CH2-O)nR2 in which n has a number-average value in the range of 5-50 and R2 is hydrogen and / or alkanyl and has a number average number of carbons in the range 0-4. Such compositions can be provided in a variety of forms. In various such embodiments, the pharmaceutical composition further includes one or more steviol glycosides mainly comprising one or more steviosides and / or rebaudiosides.

The present invention is expected to develop a novel alcoholic beverage having an ideal aroma effect. The present invention relates to an alcoholic beverage, which is characterized in that the alcoholic beverage contains steviol glycoside, and the protein content is 20-7000 mg / L.

To provide: reduced-calory or non-caloric sweeteners and sweetened compositions that have temporal and flavor profiles similar to those of sucrose and are suitable for use in consumer products; beverages or beverage products containing the compositions; and methods of improving at least one organoleptic property of beverages or beverage products.SOLUTION: A sweetener composition or a flavor modifying composition comprises (i) brazzein and (ii) at least one additional sweetener selected from a steviol glycoside sweetener and a mogroside sweetener. The steviol glycoside sweetener is selected from Rebaudioside M ("Reb M"), RebM80, Rebaudioside D ("Reb D"), A95 and Rebaudioside A ("Reb A"). The mogroside sweetener is siamenoside I.SELECTED DRAWING: None

The invention relates to the technical field of functional beverages, in particular to a composite polypeptide solid beverage with a uric acid reducing function and a preparation method of the composite polypeptide solid beverage. The composite polypeptide solid beverage with the uric acid reducing function is prepared from the following raw materials in percentage by weight: 15-25% of plukenetia volubilis extract, 30-40% of lysimachia christinae hance-plantain herb composite extract, 8-12% of oyster peptide powder, 5-8% of earthworm protein peptide freeze-dried powder and 3-5% of a flavoring agent. Wherein the ratio of a lysimachia christinae hance extract to a plantain herb extract in the lysimachia christinae hance-plantain herb composite extract is 3: The flavoring agent is erythritol and / or stevioside, xanthine oxidase is inhibited by using flavonoid substances, GLUT9 expression is synergistically down-regulated by using bispyribaloside, PRPP synthetase activity is regulated by using zinc-rich peptide, uric acid micro-crystals are dissolved by using earthworm protein peptide, a multi-target action network of flavonoid-glycoside-bioactive peptide is achieved, and the effect of improving the bioavailability of the flavonoid-glycoside-bioactive peptide is achieved. And triple mechanisms of excretion promotion, generation inhibition and kidney protection are realized.

The invention relates to the technical field of biological catalysis engineering, in particular to a method for improving the solubility of stevioside Reb M and Reb D through enzyme catalysis modification, which comprises the following steps: dissolving Reb M and Reb D with the purity of more than or equal to 90% in a buffer solution with the pH value of 4.0-8.0, adding a non-starch glycosyl donor and composite glycosyl transferase according to the enzyme-substrate mass ratio of 0.05-0.5, and optimizing the enzyme addition sequence to realize specific site modification; reacting for 6-24 hours under the conditions that the pH is 5.5-6.0 and the temperature is 60-85 DEG C, and controlling the proportion of DP2-3 to be 60-80%; hPLC sampling is carried out every 4-6 hours, and the unreacted substrate is less than or equal to 20%; the solubility of the product is greater than or equal to 25g / L, and the sweet taste of the product is pure. According to the method, the solubility of Reb M / Reb D is improved, the structure of a directional glycosylation controlled product is realized, and pure sweetness is reserved; the reaction is mild and controllable, enzyme inactivation is simple and free of residues, industrialization is easy, and stevioside application upgrading is promoted.

A method for making a highly soluble steviol glycoside composition is described. The resulting composition readily provides solutions with at least 0.5% concentration.

The utility model discloses cleaning equipment for stevioside production, and relates to the technical field of stevioside preparation. The conveying mechanism is arranged in the cleaning frame and used for conveying stevioside raw materials, the conveying mechanism comprises a driving part and a conveying belt, the driving part is arranged in the cleaning frame, the conveying belt is arranged on the driving part, and a plurality of conveying plates are fixedly connected to the conveying belt at equal intervals; and the cleaning mechanism is arranged in the cleaning frame and is used for cleaning the stevioside raw material. According to the cleaning equipment for stevioside production, the conveying mechanism is matched with the cleaning mechanism for use, raw materials of stevioside are dispersed, the effect of the cleaning process is guaranteed, the situation that the raw materials are stacked, and the overall cleaning effect is affected is avoided, the cleaning speed is high, and the cleaning efficiency is high; the water treatment device can treat water generated after raw materials are cleaned, guarantees recycling of water sources, and is simple in structure and easy and convenient to use.

The present invention provides a Stevia plant having a low ability to form pollens as compared with the wild type Stevia species. The present invention also provides a method of producing such a Stevia plant having a low ability to form pollens, and an extract or a steviol glycoside purified product obtainable from such a plant.

A taste and flavor profile modifying composition is described. The composition includes a blend of glucosylated steviol glycosides and residual steviol glycosides which can modify the intensity of a taste and / or flavor in a food or beverage product.

Consumables comprising cyclamate and a steviol glycoside sweetener or mogroside sweetener are provided herein, wherein cyclamate, when present in an amount at or below its sweetness recognition threshold concentration, enhances the sweetness of and, optionally, improves the sweetness profile the sweetener, thereby providing a consumable that tastes more like a sucrose-sweetened consumable. Methods of enhancing the sweetness of a consumable, methods of making a consumable taste more like a sucrose-sweetened consumable and methods of preparing consumables are also detailed herein.

The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with β-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided.

The present invention discloses a method for producing a target stevioside. According to the method for producing the target stevioside, the distribution change amount of the target stevioside reaches 90% or above within 2 hours, so that a precipitate containing the target stevioside is formed. The target stevioside is rebaudioside D and / or rebaudioside M. The target stevioside is rebaudioside M.

Methods of preparing steviol glycosides are described. The methods include utilizing enzyme preparations and recombinant microorganisms for converting various starting compositions to target steviol glycosides. The steviol glycosides are useful as non- caloric sweetener, flavor enhancer, sweetness enhancer, flavor stabilizer, flavoring with modifying properties (FMP), foaming suppressor and solubility enhancing agent in consumable products such as any food, beverages, pharmaceutical compositions, tobacco products, nutraceutical compositions, and oral hygiene compositions.

Methods of preparing highly purified steviol glycosides. The methods include utilizing enzyme preparations and recombinant microorganisms for converting various starting compositions to target steviol glycosides. The highly purified steviol glycosides are useful as non-caloric sweetener, flavor enhancer, sweetness enhancer, and foaming suppressor in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.

The invention provides an American ginseng and saffron crocus compound nutrient solid beverage, belongs to the technical field of nutritious foods, and aims to solve the problems of single formula and insufficient efficacy of the existing solid beverage. The solid beverage is prepared from the following components in parts by weight: 55 to 65 parts of maltodextrin, 20 to 30 parts of inulin, 10 to 20 parts of medicinal and edible traditional Chinese medicine extract, 0.1 to 0.3 part of stevioside, 0.1 to 0.3 part of vitamin C, 0.0011 to 0.0022 part of vitamin B2 and 0.02 to 0.033 part of nicotinic acid, the medicinal and edible traditional Chinese medicine extract is obtained by mixing and extracting eleven medicinal and edible traditional Chinese medicinal raw materials such as American ginseng, Chinese yam, Chinese dates, astragalus membranaceus, angelica sinensis, poria cocos, radix ophiopogonis, saffron crocus, peach kernels, rhizoma zingiberis and cinnamon. The preparation method comprises the steps of water extraction of the traditional Chinese medicinal materials, filtration, concentration, alcohol precipitation, concentration, proportioning, mixing, spray granulation, drying, packaging and the like. The beverage is scientific in formula, rich in nutrition, good in taste and suitable for daily drinking of sub-health people, and has a good market application prospect.