308 results about "Steviol glycoside" patented technology

The present disclosure provides enzymes and methods of using those enzymes to transfer sugar moieties to the substrate steviol glycosides. The designed beta-1, 3-glycosyltransferase can be used for adding a glucose monomer connected with beta-1, 3 to steviol glycosides. In particular, the designed beta-1, 3-glycosyltransferase is used in a one-pot reaction together with a beta-1, 2-glycosyltransferase and a sucrose synthase to convert stevioside and / or Reb A to Reb M.

Compared with the prior art, the traditional Chinese medicine compound oral liquid is mainly used for invigorating qi and blood, regulating yin and yang, nourishing liver and kidney, strengthening spleen and stomach, strengthening muscles and bones and the like, a plurality of traditional Chinese medicine components are compounded and decocted to obtain liquid medicine, and finally specific modified polysaccharide, steviosin, sodium benzoate and ethylparaben are added, so that the traditional Chinese medicine compound oral liquid is prepared. The modified polysaccharide is screened and optimized and is added into the compound oral liquid, and the problems of serious wall hanging and high liquid medicine waste rate in the prior art are solved by reducing the low-temperature storage wall hanging rate and the hydrolysis rate in storage stability on the premise of not influencing the medicine effect and safety.

The invention relates to the technical field of image data processing, in particular to a stevioside impurity detection method based on industrial vision, which comprises the following steps: placing a supersaturated solution in a crystallization container for cooling crystallization to obtain surface images of each detection area under different illumination angles so as to determine a gray level fluctuation coefficient of each detection area; screening a feature detection area, acquiring a surface image of the feature detection area at a feature acquisition illumination angle to identify a feature contour, acquiring feature contours at different crystallization temperatures, determining a contour growth coefficient, determining a risk tendency category of the feature contour, and determining a mode of performing impurity marking; according to the method, a high-risk region where impurities exist is rapidly identified, the impurity marking mode is adaptively adjusted according to different crystal contour features in the surface image of the stevioside crystal, and the stevioside impurity detection reliability and detection efficiency are improved.

The invention discloses a method for improving phosphorus utilization of lateolabrax japonicus and application of stevioside of the lateolabrax japonicus. The phosphorus utilization of lateolabrax japonicus is improved by adjusting a PI3K / AKT pathway. The method is simple to operate, and raw materials are easy to obtain, safe and harmless. Meanwhile, a PI3K / AKT pathway can be effectively activated, insulin resistance and lipid metabolism of lateolabrax japonicus are improved, so that the phosphorus utilization rate of lateolabrax japonicus is increased, and the growth performance of lateolabrax japonicus is improved.

The invention belongs to the technical field of biosynthesis, and particularly relates to a method for regulating and controlling stevioside production through a UDPG synthesis route. According to the invention, a high-efficiency stevioside biosynthesis system is constructed through systematic engineering modification: in the aspect of chassis cell optimization, transcription factors (AtWRKY18, AtWRKY40 and AtMYC2) for regulating and controlling MEP pathway and key genes (dxs and / or dxr) of the MEP pathway are over-expressed, so that supply of terpene precursors is enhanced; and a heterologous MVA pathway (over-expression T7-atoB-mvaS-mvaE-mvK / mvKmut-pmK-mvaD-idi) with a modular design is introduced, so that sufficient supply of the terpenoid precursor is realized. Meanwhile, the intracellular concentration of the UDPG is increased by systematically enhancing a UDPG synthesis pathway (knocking out ugd, glgc, ushA, agp, deoA, tdk, udp, or thrA, and overexpressing galU, pgm, pyrE, pyrH, ugpA, or prs), so that the production capacity of the stevioside is remarkably improved.

The invention belongs to the technical field of biosynthesis, and particularly relates to a method for regulating and controlling stevioside production by improving the utilization rate of a carbon source. According to the invention, a high-efficiency stevioside biosynthesis system is constructed through systematic engineering modification: in the aspect of chassis cell optimization, transcription factors (AtWRKY18, AtWRKY40 and AtMYC2) for regulating and controlling MEP pathway and key genes (dxs and / or dxr) of the MEP pathway are over-expressed, so that supply of terpene precursors is enhanced; and a heterologous MVA pathway (over-expression T7-atoB-mvaS-mvaE-mvK / mvKmut-pmK-mvaD-idi) with a modular design is introduced, so that sufficient supply of the terpenoid precursor is realized. Meanwhile, by knocking out related genes and bypass metabolism genes (mgsA, iclR, menA or adhE) synthesized by organic acid, a glucose transport and utilization system (fnr, fadR, ppc, zwf or pgi is knocked out, and ACS, fbaA, tpiA, glf, galP or ptsG is overexpressed, so that the utilization rate of a carbon source is further improved, and the production capacity of stevioside is remarkably improved.

The invention relates to a stevioside composition, which comprises at least one stevioside and at least one of siamenoside I, mogroside V, mogroside III-E and mogroside II-E, and is characterized in that the stevioside composition comprises at least one stevioside and at least one of siamenoside I, mogroside V, mogroside III-E and mogroside II-E; wherein the stevioside has a higher solubility in the composition than in an equivalent composition lacking siamenoside I, mogroside V, mogroside III-E, and mogroside II-E. The invention also relates to a method for preparing the stevioside composition. The composition may be an aqueous solution.

The invention relates to an engineered glycosyltransferase and a steviol glycoside glucosylation method. The present invention provides engineered glycosyltransferases (GTs), polypeptides having GT activity and polynucleotides encoding the enzymes, as well as vectors and host cells comprising the polynucleotides and polypeptides. The present invention provides engineered sucrose synthases (SuS), polypeptides having SuS activity and polynucleotides encoding the enzymes, as well as vectors and host cells comprising the polynucleotides and polypeptides. The invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to prepare products having beta-glucose ligation. The present invention also provides compositions and methods for producing rebaudioside (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The invention also provides compositions comprising the SuS enzymes and methods of using the same. Methods for producing a GT enzyme and a SuS enzyme are also provided.

The present disclosure provides a combination of steviol glycosides having various flavor characteristics. In one aspect, the present disclosure provides a rebaudioside composition comprising at least one of rebaudioside A, rebaudioside D, and rebaudioside M. The present disclosure provides a rebaudioside composition comprising at least one of rebaudioside A, rebaudioside D, and rebaudioside M. In one embodiment, the present disclosure provides a rebaudioside composition comprising rebaudioside A, rebaudioside D, and rebaudioside M. In one embodiment, the present disclosure provides a rebaudioside composition comprising rebaudioside A, rebaudioside D, and rebaudioside M. The content of rebaudioside A, rebaudioside D, and / or rebaudioside M in the rebaudioside composition of the present disclosure may be about 5%, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 95%, etc.

The invention belongs to the technical field of functional food, and particularly relates to a compound fermentation composition for regulating immunity and intestinal microecology as well as a preparation method and application of the compound fermentation composition. The invention provides a compound fermentation composition for regulating immunity and intestinal microecology. Comprising the following components: blueberry freeze-dried powder, an astragalus extract, a lycium ruthenicum extract, a day lily flower extract, grifola frondosa polysaccharide, schizochytrium limacinum powder, a red yeast rice extract, polygonatum polysaccharide, cordyceps militaris, xylooligosaccharide, yeast beta-glucan, lactoferrin, stevioside, a complex microbial inoculant and a complex enzyme. According to the compound fermentation composition disclosed by the invention, high-efficiency immunoregulation, long-acting intestinal balance and maximum component utilization are realized, and an efficient, stable and applicable solution is provided for regulating immunity and intestinal microecology.

The present invention relates to a glycosyltransferase mutant, and a composition and a method for producing steviol glycosides using the same, and more specifically, to a glycosyltransferase of rebaudioside that transfers glucose to steviol glycosides and its mutant, a recombinant strain expressing the enzymes, and a method for producing rebaudioside using the same.

The present invention is expected to develop a novel alcoholic beverage having an ideal aroma effect. The present invention relates to an alcoholic beverage, which is characterized in that the alcoholic beverage contains steviol glycoside, and the protein content is 20-7000 mg / L.

To provide: reduced-calory or non-caloric sweeteners and sweetened compositions that have temporal and flavor profiles similar to those of sucrose and are suitable for use in consumer products; beverages or beverage products containing the compositions; and methods of improving at least one organoleptic property of beverages or beverage products.SOLUTION: A sweetener composition or a flavor modifying composition comprises (i) brazzein and (ii) at least one additional sweetener selected from a steviol glycoside sweetener and a mogroside sweetener. The steviol glycoside sweetener is selected from Rebaudioside M ("Reb M"), RebM80, Rebaudioside D ("Reb D"), A95 and Rebaudioside A ("Reb A"). The mogroside sweetener is siamenoside I.SELECTED DRAWING: None

The invention relates to the technical field of functional beverages, in particular to a composite polypeptide solid beverage with a uric acid reducing function and a preparation method of the composite polypeptide solid beverage. The composite polypeptide solid beverage with the uric acid reducing function is prepared from the following raw materials in percentage by weight: 15-25% of plukenetia volubilis extract, 30-40% of lysimachia christinae hance-plantain herb composite extract, 8-12% of oyster peptide powder, 5-8% of earthworm protein peptide freeze-dried powder and 3-5% of a flavoring agent. Wherein the ratio of a lysimachia christinae hance extract to a plantain herb extract in the lysimachia christinae hance-plantain herb composite extract is 3: The flavoring agent is erythritol and / or stevioside, xanthine oxidase is inhibited by using flavonoid substances, GLUT9 expression is synergistically down-regulated by using bispyribaloside, PRPP synthetase activity is regulated by using zinc-rich peptide, uric acid micro-crystals are dissolved by using earthworm protein peptide, a multi-target action network of flavonoid-glycoside-bioactive peptide is achieved, and the effect of improving the bioavailability of the flavonoid-glycoside-bioactive peptide is achieved. And triple mechanisms of excretion promotion, generation inhibition and kidney protection are realized.

The invention relates to the technical field of biological catalysis engineering, in particular to a method for improving the solubility of stevioside Reb M and Reb D through enzyme catalysis modification, which comprises the following steps: dissolving Reb M and Reb D with the purity of more than or equal to 90% in a buffer solution with the pH value of 4.0-8.0, adding a non-starch glycosyl donor and composite glycosyl transferase according to the enzyme-substrate mass ratio of 0.05-0.5, and optimizing the enzyme addition sequence to realize specific site modification; reacting for 6-24 hours under the conditions that the pH is 5.5-6.0 and the temperature is 60-85 DEG C, and controlling the proportion of DP2-3 to be 60-80%; hPLC sampling is carried out every 4-6 hours, and the unreacted substrate is less than or equal to 20%; the solubility of the product is greater than or equal to 25g / L, and the sweet taste of the product is pure. According to the method, the solubility of Reb M / Reb D is improved, the structure of a directional glycosylation controlled product is realized, and pure sweetness is reserved; the reaction is mild and controllable, enzyme inactivation is simple and free of residues, industrialization is easy, and stevioside application upgrading is promoted.

The utility model discloses cleaning equipment for stevioside production, and relates to the technical field of stevioside preparation. The conveying mechanism is arranged in the cleaning frame and used for conveying stevioside raw materials, the conveying mechanism comprises a driving part and a conveying belt, the driving part is arranged in the cleaning frame, the conveying belt is arranged on the driving part, and a plurality of conveying plates are fixedly connected to the conveying belt at equal intervals; and the cleaning mechanism is arranged in the cleaning frame and is used for cleaning the stevioside raw material. According to the cleaning equipment for stevioside production, the conveying mechanism is matched with the cleaning mechanism for use, raw materials of stevioside are dispersed, the effect of the cleaning process is guaranteed, the situation that the raw materials are stacked, and the overall cleaning effect is affected is avoided, the cleaning speed is high, and the cleaning efficiency is high; the water treatment device can treat water generated after raw materials are cleaned, guarantees recycling of water sources, and is simple in structure and easy and convenient to use.

The present invention provides a Stevia plant having a low ability to form pollens as compared with the wild type Stevia species. The present invention also provides a method of producing such a Stevia plant having a low ability to form pollens, and an extract or a steviol glycoside purified product obtainable from such a plant.

A taste and flavor profile modifying composition is described. The composition includes a blend of glucosylated steviol glycosides and residual steviol glycosides which can modify the intensity of a taste and / or flavor in a food or beverage product.

Consumables comprising cyclamate and a steviol glycoside sweetener or mogroside sweetener are provided herein, wherein cyclamate, when present in an amount at or below its sweetness recognition threshold concentration, enhances the sweetness of and, optionally, improves the sweetness profile the sweetener, thereby providing a consumable that tastes more like a sucrose-sweetened consumable. Methods of enhancing the sweetness of a consumable, methods of making a consumable taste more like a sucrose-sweetened consumable and methods of preparing consumables are also detailed herein.

The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with β-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided.

The present invention discloses a method for producing a target stevioside. According to the method for producing the target stevioside, the distribution change amount of the target stevioside reaches 90% or above within 2 hours, so that a precipitate containing the target stevioside is formed. The target stevioside is rebaudioside D and / or rebaudioside M. The target stevioside is rebaudioside M.

Methods of preparing steviol glycosides are described. The methods include utilizing enzyme preparations and recombinant microorganisms for converting various starting compositions to target steviol glycosides. The steviol glycosides are useful as non- caloric sweetener, flavor enhancer, sweetness enhancer, flavor stabilizer, flavoring with modifying properties (FMP), foaming suppressor and solubility enhancing agent in consumable products such as any food, beverages, pharmaceutical compositions, tobacco products, nutraceutical compositions, and oral hygiene compositions.

Methods of preparing highly purified steviol glycosides. The methods include utilizing enzyme preparations and recombinant microorganisms for converting various starting compositions to target steviol glycosides. The highly purified steviol glycosides are useful as non-caloric sweetener, flavor enhancer, sweetness enhancer, and foaming suppressor in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.

The invention provides an American ginseng and saffron crocus compound nutrient solid beverage, belongs to the technical field of nutritious foods, and aims to solve the problems of single formula and insufficient efficacy of the existing solid beverage. The solid beverage is prepared from the following components in parts by weight: 55 to 65 parts of maltodextrin, 20 to 30 parts of inulin, 10 to 20 parts of medicinal and edible traditional Chinese medicine extract, 0.1 to 0.3 part of stevioside, 0.1 to 0.3 part of vitamin C, 0.0011 to 0.0022 part of vitamin B2 and 0.02 to 0.033 part of nicotinic acid, the medicinal and edible traditional Chinese medicine extract is obtained by mixing and extracting eleven medicinal and edible traditional Chinese medicinal raw materials such as American ginseng, Chinese yam, Chinese dates, astragalus membranaceus, angelica sinensis, poria cocos, radix ophiopogonis, saffron crocus, peach kernels, rhizoma zingiberis and cinnamon. The preparation method comprises the steps of water extraction of the traditional Chinese medicinal materials, filtration, concentration, alcohol precipitation, concentration, proportioning, mixing, spray granulation, drying, packaging and the like. The beverage is scientific in formula, rich in nutrition, good in taste and suitable for daily drinking of sub-health people, and has a good market application prospect.

To provide a hair treatment agent capable of flexibly changing the quality of hair and excellent in spreadability, resistance to dripping, and preparation stability, and to provide a multi-agent type hair treatment agent and a hair treatment method.SOLUTION: The hair treatment agent comprises: an ingredient (A) which is at least one selected from the group consisting of a1: heterocyclic compounds having sulfonyl group-constituting S atoms in the ring skeleton or salts thereof, a2: steviol glycosides or derivatives thereof, a3: fructans or derivatives thereof, and a4: aromatic sulfonic acids or salts thereof; and an ingredient (B) which is an anionic surfactant. The multi-agent type hair treatment agent comprises two or more hair treatment agents and includes the hair treatment agent. The method for using the multi-agent type hair treatment agent includes a first agent applying step, a first agent removing step, and a second agent applying step.SELECTED DRAWING: None

The application belongs to the technical field of steviol glycoside production, and discloses a method for preparing steviol glycoside single components by using low total glycoside mother liquor sugar, which comprises the following steps: dissolving the low total glycoside mother liquor sugar in 80-95% methanol solution, crystallizing, filtering to obtain mother liquor and filter cake, treating the filter cake with 40-50% methanol solution, stirring and crystallizing, filtering to obtain mother liquor and filter cake, drying the filter cake to obtain pure rebaudioside A; using macroporous adsorption resin to separate the two mother liquors obtained in the previous step, using 30% ethanol solution to separate the glycosides adsorbed in the macroporous adsorption resin, playing a separating role, using 50% ethanol solution added with 1‰ oil tea saponin to resolve the macroporous adsorption resin, crystallizing and recrystallizing the resolution liquid, drying the filter cake to obtain rebaudioside C, and combining the two mother liquors obtained, removing impurities, and using high-pressure chromatography to collect resolution liquid containing rebaudioside F, and drying the resolution liquid to obtain rebaudioside F. The method is simple and effective, and greatly improves the reutilization rate of low total glycoside mother liquor sugar.

Provided herein are variant uridine-5'-diphosphate glycosyltransferase polypeptides capable of producing steviol glycosides, yeast cells capable of producing steviol glycosides, and methods of making such cells. Also provided are fermentation compositions including the disclosed host cells, and related methods of producing and recovering steviol glycosides generated by the yeast cells.

The invention provides a low-calorie sweetening agent containing rubusoside and stevioside as well as a preparation method and application of the low-calorie sweetening agent, and belongs to the technical field of food additives. The low-calorie sweetening agent is prepared from the following components in parts by mass: 20 to 30 parts of rubusoside, 5 to 10 parts of stevioside, 30 to 50 parts of sugar alcohol, 0.5 to 1.5 parts of zinc citrate, 2 to 5 parts of Arabic gum and 0.2 to 0.5 part of surface modifier. The mass ratio of the rubusoside to the stevioside is (1.5-3): 1, and the purity of the rubusoside is greater than or equal to 95%; the surface modifier is used for carrying out surface hydrophobic modification coating on the low-calorie sweetening agent, and the thickness of a coating layer is less than or equal to 100nm. According to the low-calorie sweetening agent containing rubusoside and stevioside disclosed by the invention, through reasonable component matching and a fine preparation process, the low-calorie sweetening agent is excellent in the aspect of reducing bitter taste and calorie, and has good market application prospect and popularization value.

The present invention relates to a low-acid reflux, heat-stable rock sugar syrup and a preparation method thereof, belonging to the technical field of sweeteners. The rock sugar syrup is prepared from the following raw materials in parts by weight: 80 to 90 parts of rock sugar, 0.2 to 0.4 parts of steviol glycosides, 0.5 to 2 parts of maltitol, 0.5 to 2 parts of glucose syrup, 5 to 8 parts of monk fruit extract, 80 to 120 parts of purified water, and sodium citrate. The sodium citrate is used to adjust the pH value of the rock sugar syrup to 5.5 to 6.0. The monk fruit extract is a product obtained by co-fermenting the pulp and peel of fresh monk fruit with Bifidobacterium breve and Pediococcus pentosaceus, and then enzymolyzing it with ficin. The rock sugar syrup of the present invention is added with steviol glycosides and monk fruit extract, which can well maintain the stability of the rock sugar syrup, has good anti-acid reflux properties, and saves the amount of maltitol and glucose syrup.

Compositions and consumables comprising certain sweeteners and at least one flavonoid of Formula I are provided herein, wherein the at least one sweetener is selected from certain steviol glycoside and / or mogroside sweeteners. The at least one flavonoid enhances the sweetness of the consumable and / or modulates one or more taste attributes to make the consumable taste more like a sucrose-sweetened consumable. Methods of enhancing the sweetness of a consumable, methods of making a consumable taste more like a sucrose-sweetened consumable and methods of preparing consumables are also detailed herein.