14 results about "Mogroside V" patented technology

The invention relates to a stevioside composition, which comprises at least one stevioside and at least one of siamenoside I, mogroside V, mogroside III-E and mogroside II-E, and is characterized in that the stevioside composition comprises at least one stevioside and at least one of siamenoside I, mogroside V, mogroside III-E and mogroside II-E; wherein the stevioside has a higher solubility in the composition than in an equivalent composition lacking siamenoside I, mogroside V, mogroside III-E, and mogroside II-E. The invention also relates to a method for preparing the stevioside composition. The composition may be an aqueous solution.

The invention relates to the technical field of cosmetics, in particular to application of mogroside V in preparation of skin barrier repair cosmetics, the mogroside V serves as an active ingredient, the purity of the mogroside V is not lower than 30%, and the mogroside V can be prepared into various cosmetic dosage forms such as toning lotion, facial masks and essence cream. The mogroside V has no significant toxicity to keratinocytes, can accelerate skin barrier repair by promoting cell migration, has significant antioxidant activity, and can scavenge free radicals, reduce oxidative stress injury and realize synergistic effects of barrier repair and oxidation resistance and aging resistance. Besides, mogroside V is a natural water-soluble component, can be stably dispersed in a water-based formula without a solubilizer, is free of thick and sticky feeling during use, and solves the problem of skin feeling pain points of traditional repairing components. The barrier damage problems of dry skin, redness, desquamation and the like can be improved in a targeted manner, and the application prospect is wide.

The invention relates to the technical field of cosmetics, in particular to a skin care composition with a skin aging improving function, which comprises mogroside V and a golden camellia extract. The composition can significantly reduce the expression level of aging-related SA-beta-galactosidase in human skin fibroblasts, thereby effectively inhibiting skin cell aging. Mogroside V and the camellia nitidissima extract are compounded to achieve a synergistic effect, and the anti-aging effect of the composition is remarkably superior to that of a single component. The composition is safe and non-irritant, the raw materials are natural, and the composition is suitable for various cosmetic dosage forms such as essence, face cream and facial masks.

The invention relates to the technical field of natural product extraction, and particularly discloses a method for extracting active substances in momordica grosvenori by using a deep eutectic solvent. The system comprises a composite solvent constructed by three groups of specific eutectic solvents, namely levulinic acid and glycerol, choline chloride and levulinic acid, and choline chloride and citric acid; the preparation method comprises the following steps: mixing three preferable eutectic solvents according to an equal volume, adjusting the water content, performing ultrasonic-assisted extraction on the active substances in momordica grosvenori, preliminarily optimizing key parameters such as the water content, the material-liquid ratio, the extraction times, the extraction temperature and the extraction time of the extraction process through a single-factor experiment, and further optimizing through a response surface method or a machine learning algorithm. The composite solvent system provided by the invention can be used for synchronously extracting mogroside V, flavone and polysaccharide active ingredients in momordica grosvenori; in addition, the preparation method optimizes key process parameters through machine learning, and has the advantages of high extraction efficiency, good process stability and environmental friendliness.

The present application relates to the field of biological transformation of mogroside, and discloses a method for efficiently biotransforming mogroside V to prepare mogroside III E. A kind of plant endophytic fungus is used to biotransform mogroside V to obtain mogroside III E. The method is simple in operation, and the product has high conversion yield, so it can be used for large-scale preparation of mogroside III E. The method is simple, efficient, safe, green and low in cost, and provides a new method for industrial production, food production and new drug preparation.

The application discloses a beta-1, 6-glycosidic bond hydrolytic enzyme and application thereof in traditional Chinese medicine resources. The method screens a key gene sequence with a function of hydrolyzing beta-1, 6-glycosidic bond from a Candida parapsilosis L7 genome, and names the key gene sequence as CoExg1. A gene engineering bacterium capable of efficiently heterologously expressing CoExg1 is constructed by using a molecular cloning technology. After fermentation and enzyme production, an enzyme catalytic reaction system is established, and a traditional Chinese medicine active molecule in mogroside V is directionally converted, beta-1, 6-glycosidic bonds in different regions of a substrate structure are selectively hydrolyzed, and therefore, efficient and phased synthesis of different products such as sianoside I and mogroside IIIE is realized. In addition, CoExg1 can also hydrolyze beta-glycosidic bonds in the structures of icariin and lupeol, and directionally synthesize icariin II and lupeol, thereby providing important technical support for high-value utilization of traditional Chinese medicine resources and quality improvement and efficiency enhancement of traditional Chinese medicine glycosides.

This invention discloses a method for extracting mogroside V, relating to the field of biotechnology. The method involves culturing, fermenting, centrifuging, and homogenizing *E. coli* containing glycosyltransferases UGT-MS1 and UGT-MS2 to obtain an enzyme supernatant. Then, mogroside IIE, uridine diphosphate glucose, the enzyme supernatant, and salt are added to a phosphate buffer solution for enzymatic catalysis to obtain a conversion solution. Finally, the solution is ultrafiltered, loaded onto a column, eluted, and then passed through an alkaline anion exchange resin. The effluent is concentrated and dried to obtain mogroside V. The product has a high yield and is environmentally friendly.

Methods of producing sweet mogroside compounds via a biotechnological process are provided.SOLUTION: The present invention provides host cells and methods for producing mogrol glycosides, such as mogroside V (Mog. V), mogroside VI (Mog. VI), iso-mogroside V (Isomog. V), siamenoside, and glycosylation products that are minor products of Siraitiagrosvenorii. The present invention provides genetically engineered enzymes and genetically engineered host cells that produce mogrol glycosylation products, such as Mog. V, Mog. VI, and Isomog. V, in high purity and / or high yield. Further, the present technology provides methods of making products, such as foods, beverages, oral care products, sweeteners, and flavors, comprising mogrol glycosides, such as Mog. V, Mog. VI, and Isomog. V.SELECTED DRAWING: None

The application discloses a high-purity mogroside effective component gradient coupling extraction process, and creatively deeply couples three technologies of gradient low-temperature plasma wall breaking, double-medium synergistic phase change extraction and membrane coupling dynamic purification in view of the problems of low extraction rate, poor purity, easy damage of heat-sensitive components and bitter taste residue in the prior art. Through the gradient plasma treatment of 'first weak and then strong', the mild and efficient wall breaking of the cell wall is realized at <=25 DEG C. The synergistic effect of the composite enzyme solution and the dimethyl carbonate / ethanol compound extraction agent is utilized to realize the efficient enrichment of the mogroside V and the synchronous separation of the bitter impurities. Then, the double-stage membrane coupling technology of ultrafiltration-nanofiltration is used for dynamic purification at low temperature, and the traditional resin process is replaced. The process produces the technical effect of '1+1+1>3' through the cooperation of parameters and steps, the extraction rate of the mogroside V of the momordica grosvenori is >=90%, the purity of the product is >=98%, the polysaccharide retention rate is >=90%, and the bitter impurities are completely removed.

This invention discloses a compound microbial inoculant and a microbial fertilizer. The compound microbial inoculant is composed of Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, Aspergillus niger, Saccharomyces cerevisiae, and Bacillus sicca in specific weight proportions. The microbial fertilizer contains the compound inoculant, mogroside V, sodium iron ethylenediamine di-o-hydroxyphenylacetate, potassium dihydrogen phosphate, alkaline hydrolyzed modified tobacco dust, expanded perlite, and potassium humate. The preparation method includes: mixing alkaline hydrolyzed and enzymatically hydrolyzed tobacco dust with a carrier, inoculating with the compound inoculant for staged temperature-controlled fermentation; mixing the fermentation product with functional factors, followed by sodium alginate-calcium chloride gelation and chitosan coating to form microcapsule particles. This invention achieves multifunctional integration of growth promotion, disease resistance, and stress resistance induction through synergistic functional microbial community development, waste resource utilization, and slow-release encapsulation technology, resulting in a product with good stability and long-lasting fertilizer effect.

The utility model discloses an extraction device of mogroside V, and relates to the technical field of biology. An inlet of a reaction tank is respectively communicated with a fermentation liquid tank, a mogroside IIE tank, a uridine diphosphate glucose tank and a salt tank through pipelines, and an outlet of the reaction tank is communicated with a conversion liquid tank through a pipeline; an outlet of the conversion liquid tank is communicated with a macroporous resin column through a pipeline, an inlet of the macroporous resin column is communicated with an eluent tank through a pipeline, an outlet of the macroporous resin column is communicated with an eluent tank, and an outlet of the eluent tank is communicated with an anion exchange resin column through a pipeline. An outlet of the anion exchange resin column is communicated with a concentration tank, an outlet of the concentration tank is communicated with a dryer, and an outlet of the dryer is communicated with a mogroside V tank. The yield and the purity of the product are improved, and the production steps and the production period are shortened.

This invention discloses a zero-calorie sugar rich in dietary fiber and its preparation method, belonging to the field of zero-calorie sugar technology. The method includes the following steps: enzymatic hydrolysis of a mixture of inulin and resistant dextrin using inulinase and transglucosidase to obtain material A; adding dried dragon fruit peel powder to deionized water, heating and stirring evenly in a water bath, adjusting the pH of the system to 4.0-5.0, and enzymatic hydrolysis using pectin methyl esterase, xylanase, and polygalacturonase to obtain material B; mixing erythritol, mogroside V, steviol glycosides, sucralose, material A, and material B evenly to obtain a zero-calorie sugar base powder; dissolving γ-cyclodextrin in deionized water, adding anhydrous ethanol, and stirring evenly to obtain a binder; feeding the zero-calorie sugar base powder into a fluidized bed granulator, atomizing and spraying the binder, fluidizing and drying, and sieving to obtain a scientifically formulated, technologically stable, flavorful, and nutritionally healthy zero-calorie sugar rich in dietary fiber.

This application relates to the field of monk fruit extraction technology, and in particular to a production method for extracting high-purity mogroside V from monk fruit, comprising the following steps: Step 1: pretreatment and enzyme inactivation of fresh fruit; Step 2: multiple pressing, collecting the juice after each pressing and combining it to obtain fruit juice; Step 3: countercurrent extraction of the fruit pomace with a compound enzyme-assisted extraction solvent of a 0.1-0.3% concentration of compound enzyme aqueous solution; Step 4: treatment with a compound clarifying agent, collecting the centrifuged liquid; Step 5: purification using a simulated moving bed, concentrating to obtain monk fruit concentrated juice product; Step 6: concentration and desalination using a nanofiltration membrane, concentrating until the solid content reaches 30-40%; Step 7: spray drying and finished product testing, packaging and warehousing after all indicators meet the food additive standards. This application solves the problem of low efficiency caused by frequent manual shutdowns and production interruptions at a single workstation in the prior art.

The present disclosure generally relates to mogroside compositions useful for improving the flavor of certain fruit juice compositions that have certain undesirable flavors, such as excessive astringency, bitterness, or sourness, or insufficient sweetness or mouthfeel. In some embodiments, the mogroside composition is monk fruit juice or monk fruit juice concentrate. In some embodiments, the mogroside composition comprises mogroside V, and, optionally, one or more other naturally occurring mogrosides.