40 results about "Mogroside V" patented technology

The invention relates to a stevioside composition, which comprises at least one stevioside and at least one of siamenoside I, mogroside V, mogroside III-E and mogroside II-E, and is characterized in that the stevioside composition comprises at least one stevioside and at least one of siamenoside I, mogroside V, mogroside III-E and mogroside II-E; wherein the stevioside has a higher solubility in the composition than in an equivalent composition lacking siamenoside I, mogroside V, mogroside III-E, and mogroside II-E. The invention also relates to a method for preparing the stevioside composition. The composition may be an aqueous solution.

The invention discloses saccharomyces cerevisiae engineering bacteria for efficiently synthesizing mogroside V on the basis of cytoplasm and lipid droplet double-cell area compartment and a construction method of the saccharomyces cerevisiae engineering bacteria. The construction method comprises the following steps: integrating truncated 3-hydroxy-3-methylglutaryl coenzyme A reductase tHMG1, pentenyl pyrophosphate isomerase IDI1, epoxy squalene cyclization enzyme ERG1, squalene synthetase ERG9, choline kinase ScCK, isopentenyl phosphokinase AtIPK, cucurbitadienol synthetase SgCDS, cyclic epoxy hydrolase SgEPH3, cytochrome P450 enzyme CYP87D18 and cytochrome P450 enzyme reductase AtCPR1 at the site of a saccharomyces cerevisiae genome GAL80; an ABC efflux protein PDR11, a glycosyl transferase UGTMG1, a sucrose synthase Susy and a glycosyl transferase SgUGT94-289-3 are integrated at a site Exg1 of a saccharomyces cerevisiae genome. The yield of mogroside V produced by shake flask fermentation of the saccharomyces cerevisiae engineering strain provided by the invention reaches 96.3 mg / L, so that mogroside V is efficiently synthesized from the beginning in the saccharomyces cerevisiae, and the saccharomyces cerevisiae engineering strain has important practical significance for promoting sustainable development of the mogroside industry.

The invention provides a pulmonary nodule targeting food and medicine homologous composition based on metabonomics screening and application thereof. By analyzing a pulmonary nodule specific metabolic profile, active components, namely platycodin D, mogroside V, oyster active peptide and fermented cordycepin, which can cooperatively regulate and control an arachidonic acid inflammation pathway, a glutathione antioxidant pathway and a tricarboxylic acid cycle energy pathway are reversely screened out. Animal experiments prove that the composition can reduce the volume of pulmonary nodules by 58.3%, human body application shows that the nodules of 76.7% of subjects are reduced, and liver and kidney toxicity is avoided. The composition can be prepared into tablets, capsules and other dosage forms, can be used as functional food or adjuvant therapy drugs for high risk groups of pulmonary nodules, and realizes accurate nutrition intervention.

The invention discloses a saccharomyces cerevisiae genetically engineered bacterium for efficiently synthesizing mogroside V based on multi-compartment multi-metabolic pathways and a construction method of the saccharomyces cerevisiae genetically engineered bacterium. The preparation method comprises the following steps: anchoring an MVA pathway and a squalene synthesis pathway on the surface of a peroxisome membrane, and enabling mogrol synthetase and epoxy squalene cyclization enzyme ERG1 to form a linear polywheat complex through polypeptide interaction; then, through overexpression of MVA pathway key enzyme and introduction of an isopentenol utilization pathway, supply of precursor isopentenyl pyrophosphate and dimethyl allyl pyrophosphate in cytoplasm is increased, and squalene epoxidase ERG1 and mogrol synthetase are anchored in transmembrane ABC efflux protein PDR11 through polypeptide interaction; then anchoring the mogrol synthetase in the lipid droplet subcells; finally, the glucosyltransferase is displayed on the surface of the yeast cell wall through an alpha-lectin system, so that extracellular mogrol is converted into MG-V. The engineering bacterium improves the yield of mogroside V synthesized from the beginning in microorganisms, and has great industrial application value.

To provide alternative and / or improved sweetness modifying composition.SOLUTION: There is provided a sweetness modifying composition comprising: one or more high-intensity sweetener(s) selected from steviol glycosides and / or mogrosides; and one or more low-intensity sweetener(s) selected from cellobiose, psicose, cyclamate and / or 11-O-mogroside V, wherein the sweetness modifying composition increases the sweetness of a sweetened composition by more than the sweetness of the sweetness modifying composition alone; and / or wherein the ratio of the one or more high-intensity sweetener(s) to the one or more low-potency sweetener(s) ranges from about 2:1 to about 12:1.SELECTED DRAWING: None

The present disclosure provides compositions and methods for Agrobacterium infiltration of plant tissues, for example fruit, vegetable or leaf tissues, and the use of such plant tissues for production of high-value compounds, for example mogroside compounds, in particular mogroside V, betalain, lactoferrin, or fluorescent proteins, such as green fluorescent protein (GFP), and the high-value compounds so produced.

The present disclosure provides compositions and methods for producing transgenic plants and other organisms that exhibit increased production of mogroside compounds, in particular mogroside V, and the mogroside compounds, plants and plant parts so produced.

The present invention provides host cells and methods for making mogrol glycosides, including Mogroside V (Mog.V), Mogroside VI (Mog.VI), Iso-Mogroside V (Isomog.V), siamenoside, and glycosylation products that are minor products in Siraitia grosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosylation products, such as Mog.V, Mog.VI, and Isomog.V, at high purity and / or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog.V, Mog.VI, and Isomog.V, including food products, beverages, oral care products, sweeteners, and flavoring products.

The invention discloses saccharomyces cerevisiae engineering bacteria for efficiently synthesizing mogroside V based on lipid droplet subcellular organelles and a construction method of the saccharomyces cerevisiae engineering bacteria. The construction method comprises the following steps: integrating squalene epoxidase ERG1, cucurbitadienol synthetase SgCDS, cyclic epoxy hydrolase SgEPH3, cytochrome P450 enzyme CYP87D18 and cytochrome P450 enzyme reductase AtCPR1 at a site GAL80 of a saccharomyces cerevisiae genome; the saccharomyces cerevisiae strain is characterized in that phosphoglucose mutase PGM1, alpha-phosphoglucose mutase PGM2, uridine diphosphate glucose pyrophosphorylase UGP1, glycosyl transferase UGTMG1 and glycosyl transferase SgUGT94-289-3 are integrated at a site Exg1 of a saccharomyces cerevisiae genome. The Saccharomyces cerevisiae engineering bacterium constructed by the invention realizes that the yield of MG-V synthesized by shake flask fermentation is 31.4 mg / L. The Saccharomyces cerevisiae engineering bacterium has the characteristics of strong metabolic flux directionality, high yield and wide industrial application prospect.

The invention relates to the technical field of cosmetics, in particular to application of mogroside V in preparation of skin barrier repair cosmetics, the mogroside V serves as an active ingredient, the purity of the mogroside V is not lower than 30%, and the mogroside V can be prepared into various cosmetic dosage forms such as toning lotion, facial masks and essence cream. The mogroside V has no significant toxicity to keratinocytes, can accelerate skin barrier repair by promoting cell migration, has significant antioxidant activity, and can scavenge free radicals, reduce oxidative stress injury and realize synergistic effects of barrier repair and oxidation resistance and aging resistance. Besides, mogroside V is a natural water-soluble component, can be stably dispersed in a water-based formula without a solubilizer, is free of thick and sticky feeling during use, and solves the problem of skin feeling pain points of traditional repairing components. The barrier damage problems of dry skin, redness, desquamation and the like can be improved in a targeted manner, and the application prospect is wide.

The invention relates to the technical field of cosmetics, in particular to a skin care composition with a skin aging improving function, which comprises mogroside V and a golden camellia extract. The composition can significantly reduce the expression level of aging-related SA-beta-galactosidase in human skin fibroblasts, thereby effectively inhibiting skin cell aging. Mogroside V and the camellia nitidissima extract are compounded to achieve a synergistic effect, and the anti-aging effect of the composition is remarkably superior to that of a single component. The composition is safe and non-irritant, the raw materials are natural, and the composition is suitable for various cosmetic dosage forms such as essence, face cream and facial masks.

The present application relates to a sweet composition, more particularly to a sweet composition and its preparation method and use. The sweet composition comprises at least 0-99% mogroside V and 0-99% fructooligosaccharide. The preparation method comprises taking a mogroside extract, and then performing enzymatic conversion treatment on the mogroside extract by using a biological enzyme coupling membrane separation technology. Under the action of fructose transmembrane enzyme, sucrose enzyme and glucose isomerase, sucrose in the mogroside is converted into fructooligosaccharide, and the generally existing low-sweetness glucose in the mogroside is converted into high-sweetness fructose. Then, after recombination, concentration and sterilization, the sweet composition is obtained. The mogroside sweet composition product prepared by the present application not only has low sugar and low heat, but also has purer taste, fuller flavor, faster dissolution, and is closer to edible sugar. The defects of insufficient sweetness and slow sweetness of mogroside V after sweetness are improved, and the mogroside sweet composition can be applied to the preparation of food, beverage, dietary supplement, medicine and pet food.

The invention discloses a saccharomyces cerevisiae engineering bacterium for efficiently synthesizing mogroside V based on peroxisome and lipid droplet double-cell area compartment and a construction method of the saccharomyces cerevisiae engineering bacterium. According to the construction method disclosed by the invention, squalene epoxidase ERG1 is anchored on the surface of a peroxisome membrane and in lipid droplets respectively; the ERG1 and downstream metabolic enzymes such as cucurbitadienol synthetase, cyclic epoxy hydrolase, cytochrome P450 enzyme, cytochrome P450 enzyme reductase, phosphoglucomutase, alpha-phosphoglucomutase, uridine diphosphate glucose pyrophosphorylase and glycosyltransferase are assembled into a multi-enzyme complex by adopting a multi-enzyme complex directional assembly strategy; the method not only enhances the directionality of metabolic flux, but also improves the catalytic efficiency of the enzyme and reduces the generation of byproducts, thereby realizing efficient synthesis of MG-V.

The invention discloses application of mogroside V in preparation of an anti-renal fibrosis medicine, and belongs to the technical field of biological medicines. Experiments prove that the mogroside V can obviously improve the renal function of a mouse with renal fibrosis, and the serum creatinine and blood urea nitrogen levels are obviously reduced. Meanwhile, the compound can be used for effectively inhibiting the expression of fibrosis markers alpha-SMA and Fibronectin in renal tissues, and delaying the fibrosis process by up-regulating the expression level of an epithelial-mesenchymal transition marker E-cadherin. In addition, the mogroside V can also significantly reduce collagen deposition in kidney tissues, and plays a role in treating renal fibrosis. The traditional Chinese medicine composition has the advantages of safety, effectiveness, no side effect, good taste and the like when being used for preparing the anti-renal fibrosis medicine.

The invention discloses a saccharomyces cerevisiae engineering bacterium for efficiently synthesizing mogroside V based on cytoplasm and peroxisome double-cell-region indoor and a construction method of the saccharomyces cerevisiae engineering bacterium. Wherein the construction method comprises the following steps: integrating acetoacetyl coenzyme A thiolase ERG10, 3-hydroxy-3-methylglutaryl coenzyme A synthetase ERG13, truncated 3-hydroxy-3-methylglutaryl coenzyme A reductase tHMG1, phosphomevalonate kinase ERG8, mevalonate pyrophosphate decarboxylase ERG19, pentenyl pyrophosphate isomerase IDI1 and geranyl pyrophosphate synthase ERG20 at a site of a saccharomyces cerevisiae genome GAL80, and carrying out enzymolysis, so as to obtain a saccharomyces cerevisiae strain; a squalene synthase ERG9, an epoxy squalene cyclization enzyme ERG1 and the like; aBC efflux protein PDR11, isopentene phosphokinase AtIPK, geranyl pyrophosphate synthase ERG20, endogenous squalene synthetase ERG9, epoxy squalene cyclization enzyme ERG1 and the like are integrated at a site Exg1 of a saccharomyces cerevisiae genome. The yield of MG-V synthesized by shake flask fermentation of the saccharomyces cerevisiae engineering bacteria constructed by the invention reaches 117.6 mg / L, and the saccharomyces cerevisiae engineering bacteria have the advantages of high yield, strong metabolic flux directionality, wide industrial application prospect and the like.

The present invention relates to a sweetener concentrate composition comprising erythritol, one or more steviosides, sucralose, a siraitia grosvenorii extract containing at least 45% by mass of mogroside V, acid, siraitia grosvenorii juice, potassium sorbate and water. The invention also relates to a beverage product comprising the sweetener concentrate composition and a method of sweetening a beverage composition.

The invention discloses a saccharomyces cerevisiae genetically engineered bacterium for efficiently synthesizing mogroside V through multi-dimensional cytoplasm adaptability and a construction method of the saccharomyces cerevisiae genetically engineered bacterium. The construction method of the saccharomyces cerevisiae genetically engineered bacterium comprises the following steps: integrating truncated 3-hydroxy-3-methylglutaryl coenzyme A reductase tHMG1, pentenyl pyrophosphate isomerase IDI1, squalene synthetase ERG9, epoxy squalene cyclization enzyme ERG1, choline kinase ScCK, isopentenyl phosphokinase AtIPK, phosphoketolase BbPK, phosphotransacetylase PTA and dihydroxyacetone hydrolase EcHAD at a site of a saccharomyces cerevisiae genome GAL80, and carrying out enzymolysis, so as to obtain the saccharomyces cerevisiae genetically engineered bacterium. The invention relates to a preparation method of a cytochrome P450 enzyme, which comprises the following steps of: preparing a cycloepoxide hydrolase SgEPH3, a cytochrome P450 enzyme CYP87D18 and a cytochrome P450 enzyme reductase AtCPR1; an ABC efflux protein PDR11, a glycosyl transferase UGTMG1, a sucrose synthase Susy and a glycosyl transferase SgUGT94-289-3 are integrated at a site Exg1 of a saccharomyces cerevisiae genome. The saccharomyces cerevisiae genetically engineered bacterium constructed by the invention realizes a higher level of mogroside V yield synthesized from the beginning in microorganisms, and has important industrial application potential.

The invention relates to the technical field of natural product extraction, and particularly discloses a method for extracting active substances in momordica grosvenori by using a deep eutectic solvent. The system comprises a composite solvent constructed by three groups of specific eutectic solvents, namely levulinic acid and glycerol, choline chloride and levulinic acid, and choline chloride and citric acid; the preparation method comprises the following steps: mixing three preferable eutectic solvents according to an equal volume, adjusting the water content, performing ultrasonic-assisted extraction on the active substances in momordica grosvenori, preliminarily optimizing key parameters such as the water content, the material-liquid ratio, the extraction times, the extraction temperature and the extraction time of the extraction process through a single-factor experiment, and further optimizing through a response surface method or a machine learning algorithm. The composite solvent system provided by the invention can be used for synchronously extracting mogroside V, flavone and polysaccharide active ingredients in momordica grosvenori; in addition, the preparation method optimizes key process parameters through machine learning, and has the advantages of high extraction efficiency, good process stability and environmental friendliness.

The present application relates to the field of biological transformation of mogroside, and discloses a method for efficiently biotransforming mogroside V to prepare mogroside III E. A kind of plant endophytic fungus is used to biotransform mogroside V to obtain mogroside III E. The method is simple in operation, and the product has high conversion yield, so it can be used for large-scale preparation of mogroside III E. The method is simple, efficient, safe, green and low in cost, and provides a new method for industrial production, food production and new drug preparation.

The application discloses a beta-1, 6-glycosidic bond hydrolytic enzyme and application thereof in traditional Chinese medicine resources. The method screens a key gene sequence with a function of hydrolyzing beta-1, 6-glycosidic bond from a Candida parapsilosis L7 genome, and names the key gene sequence as CoExg1. A gene engineering bacterium capable of efficiently heterologously expressing CoExg1 is constructed by using a molecular cloning technology. After fermentation and enzyme production, an enzyme catalytic reaction system is established, and a traditional Chinese medicine active molecule in mogroside V is directionally converted, beta-1, 6-glycosidic bonds in different regions of a substrate structure are selectively hydrolyzed, and therefore, efficient and phased synthesis of different products such as sianoside I and mogroside IIIE is realized. In addition, CoExg1 can also hydrolyze beta-glycosidic bonds in the structures of icariin and lupeol, and directionally synthesize icariin II and lupeol, thereby providing important technical support for high-value utilization of traditional Chinese medicine resources and quality improvement and efficiency enhancement of traditional Chinese medicine glycosides.

Monk fruit extracts containing mogrol glycosides such as mogroside V may be treated with enzymes under specific reaction conditions to shift distribution of the Siamenoside I reaction product. Modified enzymes are also employed to shift Siamenoside I distribution to increase yield of Siamenoside I and reduce reaction contaminants. Methods of purifying bioconversion reaction product are also described. Siamenoside I obtained using these methods is a useful sweetener and flavor enhancer for food and beverage compositions and the like.

The present invention relates to an engineered yeast capable of producing mogrol, siamenoside I, mogroside IIIX, mogroside IVA, and / or mogroside V and the use thereof. The present invention provides an engineered yeast capable of producing mogrol, siamenoside I, mogroside IIIX, mogroside IVA, and / or mogroside V. By optimizing and modifying chassis cells, a metabolic pathway of microorganisms is changed, or an expression level of related genes is regulated, thereby enhancing the synthesis efficiency and yield of target products, while improving the production efficiency.

This invention discloses a method for extracting mogroside V, relating to the field of biotechnology. The method involves culturing, fermenting, centrifuging, and homogenizing *E. coli* containing glycosyltransferases UGT-MS1 and UGT-MS2 to obtain an enzyme supernatant. Then, mogroside IIE, uridine diphosphate glucose, the enzyme supernatant, and salt are added to a phosphate buffer solution for enzymatic catalysis to obtain a conversion solution. Finally, the solution is ultrafiltered, loaded onto a column, eluted, and then passed through an alkaline anion exchange resin. The effluent is concentrated and dried to obtain mogroside V. The product has a high yield and is environmentally friendly.

Methods of producing sweet mogroside compounds via a biotechnological process are provided.SOLUTION: The present invention provides host cells and methods for producing mogrol glycosides, such as mogroside V (Mog. V), mogroside VI (Mog. VI), iso-mogroside V (Isomog. V), siamenoside, and glycosylation products that are minor products of Siraitiagrosvenorii. The present invention provides genetically engineered enzymes and genetically engineered host cells that produce mogrol glycosylation products, such as Mog. V, Mog. VI, and Isomog. V, in high purity and / or high yield. Further, the present technology provides methods of making products, such as foods, beverages, oral care products, sweeteners, and flavors, comprising mogrol glycosides, such as Mog. V, Mog. VI, and Isomog. V.SELECTED DRAWING: None

The invention discloses beta-1, 6-glucosidic bond hydrolase and application thereof in traditional Chinese medicine resources. According to the method, a key gene sequence with a beta-1, 6-glucosidic bond hydrolysis function is screened from a Candida pseudoglabrata L7 genome and is named as CoExg1. A gene engineering bacterium capable of efficiently and heterologously expressing CoExg1 is constructed by utilizing a molecular cloning technology. According to the method, siamenoside I and mogroside IIIE are used as raw materials, an enzyme method catalytic reaction system is established after enzyme production through fermentation, traditional Chinese medicine active molecules of mogroside V are directionally converted, beta-1, 6-glucosidic bonds in different areas in a substrate structure are selectively hydrolyzed, and therefore efficient staged synthesis of different products such as siamenoside I and mogroside IIIE is achieved. Besides, the CoExg1 can hydrolyze beta-glucosidic bonds in isopentenyl flavone icariin and formononetin structures, icariside II and formononetin are respectively and directionally synthesized, and an important technical support is provided for high-value utilization of traditional Chinese medicine resources and quality and efficiency improvement of traditional Chinese medicine glycoside.

The application discloses a high-purity mogroside effective component gradient coupling extraction process, and creatively deeply couples three technologies of gradient low-temperature plasma wall breaking, double-medium synergistic phase change extraction and membrane coupling dynamic purification in view of the problems of low extraction rate, poor purity, easy damage of heat-sensitive components and bitter taste residue in the prior art. Through the gradient plasma treatment of 'first weak and then strong', the mild and efficient wall breaking of the cell wall is realized at <=25 DEG C. The synergistic effect of the composite enzyme solution and the dimethyl carbonate / ethanol compound extraction agent is utilized to realize the efficient enrichment of the mogroside V and the synchronous separation of the bitter impurities. Then, the double-stage membrane coupling technology of ultrafiltration-nanofiltration is used for dynamic purification at low temperature, and the traditional resin process is replaced. The process produces the technical effect of '1+1+1>3' through the cooperation of parameters and steps, the extraction rate of the mogroside V of the momordica grosvenori is >=90%, the purity of the product is >=98%, the polysaccharide retention rate is >=90%, and the bitter impurities are completely removed.

The invention discloses a loose nanofiltration membrane for purifying and separating mogroside V and a preparation method of the loose nanofiltration membrane. According to the method, a polyethersulfone ultrafiltration membrane is used as a supporting base material, and a loose polyamide nanofiltration membrane is generated after a liquid-liquid interface polymerization reaction is carried out between a piperazine aqueous solution and a trimesoyl chloride solution. Under the optimized conditions of 6 bar, 80 DEG C and 3-degree Bx, the permeation flux of the synthetic membrane to mogroside V reaches 80.95 L m <-2 > h <-1 > bar <-1 >, and the retention rate is maintained at 98.0% or above. The method developed by the invention is simple, rapid and stable in performance, the prepared loose nanofiltration membrane has high permeation flux and high rejection rate when being used for purifying and separating mogroside V, and the purification and separation technology provides reference for the practical application of the momordica grosvenori extraction industry.

A low calorie beverage sweetened with a mogroside blend sweetener comprising siamenoside I, with improved sensory properties is described. Isomogroside V, 11-oxo mogroside V, mogroside III, mogroside IIE, mogroside V, and mogroside IIIE, when blended with siamenoside I in a certain ratio, can be used to reduce bitterness, sweet retention, bitterness retention, metallic taste, and / or astringency when used in sweetened beverages compared to beverages sweetened only with siamenoside I.

The invention discloses saccharomyces cerevisiae engineering bacteria for efficiently synthesizing mogroside V based on peroxisome subcellular organelles and a construction method of the saccharomyces cerevisiae engineering bacteria. A construction method of the engineering bacterium comprises the following steps: (1) integrating mogrol metabolic enzyme and leucine selection marker Leu2 at a site of a transcription inhibition factor GAL80 of saccharomyces cerevisiae; and (2) integrating peroxisome membrane protein Pex11, carnitine acetyltransferase Cat2, phosphoglucose mutase PGM1, alpha-phosphoglucose mutase PGM2, uridine diphosphate glucose pyrophosphorylase UGP1, glycosyl transferase UGTMG1, glycosyl transferase SgUGT94-289-3 and a histidine selection marker His3 at the site of the saccharomyces cerevisiae glucoside hydrolase Exg1. The engineering strain has the advantages of high yield, strong metabolic flux directionality, wide industrial application prospect and the like, and a new technical route is provided for microbial manufacturing of natural sweeteners.

The invention discloses application, a preparation method and a composition of mogroside, and relates to the technical field of biological medicines. Specifically, the invention relates to the application of mogroside and / or a derivative of mogroside in improving the stability of NMN and increasing the conversion rate of NMN into NAD + in / out of cells. The invention finds that mogroside can improve the stability of NMN for the first time, and also finds that mogroside, especially mogroside V in mogroside, can improve the content of NAD + in / out of cells and improve the conversion rate of NMN into NAD + in / out of cells, thereby effectively preventing body aging and viscera function degeneration, and improving the activity of NMN in the cells. And a theoretical reference value is provided for clinical medicine.