Method for extracting high-purity mogroside V from siraitia grosvenorii

A high-purity technology of mogroside, applied in food extraction, chemical instruments and methods, glycoside steroids, etc., can solve the problems of cumbersome separation and purification of macroporous resin, large amount of organic solvent used, low yield of mogroside V, etc. To achieve product safety and environmental protection, the effect is obvious, and the extraction time is shortened

Active Publication Date: 2016-10-12
JIANGXI HAIFU BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, column chromatography is the most widely used method, and the separation and purification of macroporous adsorption resins are the most widely used. Resin types such as D101, AB-8, etc., but the separation and purification of macroporous resins is cumbersome, and the yield of mogroside V is low. And elution requires the use of organic solvents, resulting in solvent residues and environmental pollution, etc.
Preparative chromatography and high-speed countercurrent chromatography have high product content, but the one-time investment cost is large, and the amount of organic solvent used is large, and there are still problems such as solvent residue and environmental pollution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1) Pretreatment of raw materials: 1000Kg of fresh fruit raw materials with impurities are crushed with a crusher, and added to the continuous countercurrent extraction unit while crushing.

[0033] 2) Extraction: At the same time, add 2000kg of 96°C hot pure water in reverse direction for continuous countercurrent extraction, and set the extraction time to 1h.

[0034] 3) Centrifugation: centrifuge the extract obtained in step (2) with a decanter centrifuge, cool it to 30°C, and then pass it through a disc centrifuge to obtain 2158kg of centrifuge, and the temperature of the feed liquid is 32°C.

[0035] 4) Enzymolysis: Put 2158kg of centrifuge liquid obtained in step (3) into an enzymolysis tank, add cellulase, pectinase, and protease compound enzyme for enzymolysis (the compound enzyme is an imported Novozymes liquid enzyme), compound The amount of enzyme added is 0.01% of the volume of the extract, the enzymolysis temperature is controlled at 32°C, the enzymolysis ti...

Embodiment 2

[0042] 1) Pretreatment of raw materials: 1000Kg of fresh fruit raw materials with impurities are crushed with a crusher, and added to the continuous countercurrent extraction unit while crushing.

[0043]2) Extraction: At the same time, 3000kg of 98°C hot pure water was added in reverse direction for continuous countercurrent extraction, and the extraction time was set to 1.5h.

[0044] 3) Centrifugation: centrifuge the extract obtained in step (2) with a decanter centrifuge, cool it to 35°C, and then pass it through a disc centrifuge to obtain 3080kg of centrifuge, and the temperature of the feed liquid is 38°C.

[0045] 4) Enzymatic hydrolysis: Put 3080kg of centrifugation liquid obtained in step (3) into an enzymatic hydrolysis tank, add cellulase, pectinase, and protease compound enzyme for enzymolysis (the compound enzyme is an imported Novozymes liquid enzyme), compound The amount of enzyme added is 0.02% of the volume of the extract, the enzymolysis temperature is contr...

Embodiment 3

[0052] 1) Pretreatment of raw materials: crush 1000Kg of fresh fruit raw materials with impurities with a crusher, and add them to the continuous countercurrent extraction unit while crushing.

[0053] 2) Extraction: At the same time, add 4000kg of hot pure water at 98°C in reverse direction for continuous countercurrent extraction, and set the extraction time to 1.5h.

[0054] 3) Centrifugation: The extract obtained in step (2) was centrifuged with a decanter centrifuge, cooled to 42°C, and then passed through a disc centrifuge to obtain 4176kg of centrifuge, and the temperature of the feed liquid was 44°C.

[0055] 4) Enzymolysis: Put 4176kg of centrifuge liquid obtained in step (3) into an enzymolysis tank, add cellulase, pectinase, and protease compound enzyme for enzymolysis (the compound enzyme is an imported Novozymes liquid enzyme), compound The amount of enzyme added is 0.03% of the volume of the extract, the temperature of enzymolysis is controlled at 44°C, the time ...

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Abstract

The invention discloses a method for extracting high-purity mogroside V from siraitia grosvenorii, and relates to the extraction method of the mogroside V. The method includes the specific steps: raw material pretreatment, extraction, centrifugation, enzymolysis, ultrafiltration, nanofiltration, decolorization, concentration, microwave drying, and crushing. The membrane technique is adopted for separation and purification, only pure water is used as a solvent, an organic solvent is discarded, and the method has the advantages of simple operation, safe and environmental-protection products, simple process, high quality and low price, and can realize the continuous large-scale industrial production.

Description

technical field [0001] The invention relates to a method for extracting mogroside V, in particular to a method for extracting high-purity mogroside V from Luo Han Guo. Background technique [0002] Luo Han Guo (scientific name: Siraitia grosvenorii) is a perennial vine of Cucurbitaceae. Its leaves are heart-shaped, dioecious, flowering in summer and fruiting in autumn. Traditional Chinese medicine uses its fruit as medicine, which contains mogroside, various amino acids and vitamins and other medicinal ingredients. Luo Han Guo, also known as sweetener, is widely used as a sweetener because of its high sweetness and low calories. It is often used as a sugar substitute for obese and diabetic patients. [0003] Patent No. CN200910044558.9 provides a production method of Luo Han Guo extract with mogroside V content greater than 60%: crushing→saccharification→water extraction→concentration→sedimentation centrifugation→ion exchange resin refining→macroporous resin refining→conce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00
CPCC07J17/005B01D61/027B01D61/145B01D2311/04A23L27/36B01D2311/06B01D2311/08B01D61/58A23V2002/00A23V2300/10A23V2300/14A23V2300/24A23V2300/34A23V2300/38
Inventor 龙伟岸黄华学
Owner JIANGXI HAIFU BIOENG
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