The invention discloses a method for removing
ginkgolic acid in a water-soluble
ginkgo leaf extract, which comprises the following steps: mixing the
ginkgo leaf extract with a compound
enzyme solution, fully stirring for enzymolysis, adding a modified
fullerene-
graphene oxide compound for adsorption, then carrying out
electrodialysis separation, then carrying out
chromatographic column adsorption, refrigerating, crystallizing and removing impurities, thus obtaining the
ginkgolic acid in the water-soluble
ginkgo leaf extract. And performing membrane concentration and
drying to obtain the
ginkgo biloba extract without
ginkgolic acid. According to the method, a revolutionary breakthrough is realized in the field of purification of the water-soluble
ginkgo leaf extract by constructing a synergistic
system of biological enzymolysis, nano material adsorption, electrochemical separation and a
molecular recognition technology. Compared with a traditional process, the scheme creatively adopts a composite enzymolysis pretreatment technology, and creates favorable conditions for subsequent purification; the innovatively designed modified
fullerene-
graphene oxide nano
composite material can be used for efficiently capturing free ginkgolic acid under a mild condition; gradient removal of ginkgolic acid is achieved through a unique
electrodialysis-
molecular imprinting coupling technology, the removal efficiency is guaranteed, and
flavonoid active ingredients are protected.