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Production of oil seed protein isolate

A technology for protein separation and oilseed meal, applied in protein food ingredients, protein composition of vegetable seeds, plant protein processing, etc., can solve the problem of not providing data on product purity

Inactive Publication Date: 2004-08-25
BURCON NUTRASCI MB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Murray IA patent describes an experiment involving rapeseed, but provides no data on the purity of the product

Method used

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  • Production of oil seed protein isolate
  • Production of oil seed protein isolate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] This example illustrates the method of the invention.

[0065] At room temperature, "a" Kg commercial canola coarse powder was added to "b"L 0.15mol / L NaCl solution, and stirred for 30 minutes to form a protein aqueous solution with a protein content of "c" g / L. Canola meal residue was removed and washed on a vacuum filter belt. The resulting protein solution was clarified by centrifugation, yielding "d" L of clarified protein solution having a protein content of "e" g / L.

[0066] The volume of the protein extract or "f" L of the protein extract is reduced to "g" L by an ultrafiltration system using a membrane with a molecular weight cut-off of "h" Daltons. The resulting concentrated protein solution contains "i" g / L of protein.

[0067] The concentrated solution at temperature "j"°C is diluted "k" times with water at 4°C. A white cloud of protein micelles immediately forms and settles. The upper layer was removed for dilution with water, and a precipitated, viscous...

Embodiment 2

[0074] The process of Example 1 was repeated by changing the conditions in the above method. Several parameters were investigated.

[0075] (a) Extract parameters

[0076] The extraction parameters were varied to determine their effect on the obtained concentrated protein solution. The results are shown in Table II.

[0077] Table II

[0078] Extraction concentration

extraction temperature

extraction time

NaCl solution

concentration

extract

pH

protein concentration

5%w / v

13℃

30min

0.15M

6.4

5.3g / L

15%w / v

13℃

30min

0.15M

6.2

12.7g / L

15%w / v

8℃

30min

0.15M

-

6.6g / L

15%w / v

34℃

30min

0.15M

-

14.6g / L

15%w / v

22℃

10min

0.15M

5.9

10.5g / L

15%w / v

13℃

60min

0.15M

5.9

10.6g / L

[0079] ...

Embodiment 3

[0085] This example illustrates the effect of the temperature of the dilution water on the yield of protein isolate produced.

[0086] At room temperature, 1200Kg of commercial canola coarse powder was added into 8000L of 0.15mol / L NaCl solution, and stirred for 30 minutes to form a protein aqueous solution with a protein content of 17.4g / L. Canola meal residue was removed and washed on a vacuum filter belt. The resulting protein solution was clarified by centrifugation yielding 7464 L of clear protein solution with a protein content of 14.8 g / L.

[0087] Using a membrane with a molecular weight cut-off of 3000 Daltons, the volume of the concentrated protein extract is reduced through an ultrafiltration system. The resulting concentrated protein solution had a protein content of 230 g / L.

[0088] 50 ml aliquots of the concentrated solution were warmed to 30°C and then diluted 1:10 into tap water at 15°C. A small amount of white cloud-like material composed of very small mic...

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Abstract

Oil seed protein isolates, particularly canola protein isolate, are produced at a high purity level of at least about 100 wt % (Nx6.25) by a process wherein oil seed protein is extracted from oil seed meal, the resulting aqueous protein solution is concentrated to a protein content of at least about 200 g / L and the concentrated protein solution is added to chilled water having a temperature below about 15 DEG C. to form protein micelles, which are settled to provide a protein micellar mass (PMM). The protein micelar mass is separated from supernatant and may be dried. The supernatant may be processed to recover additional oil seed protein isolate by concentrating the supernatant and then drying the concentrated supernatant, to produce a protein isolate having a protein content of at least about 90 wt %. The concentrated supernatant may be mixed in varying proportions with at least part of the PMM and the mixture dried to produce a protein isolate having a protein content of at least about 90 wt %.

Description

[0001] related application [0002] Pursuant to 35 USC 119(e), this application claims priority to the following U.S. patent applications: 60 / 288,415 filed May 4, 2001, 60 / 326,987 filed October 5, 2001, and November 7, 2001 60 / 331,066 filed on 26 November 2001, 60 / 333,494 filed on 26 November 2001, and ______ filed on _____. field of invention [0003] The present invention relates to improved methods of producing oilseed protein isolates, particularly canola protein isolates. Background technique [0004] U.S. Patent Nos. 5,844,086 and 6,005,076 ("Murray II") (already assigned to the assignee of the present invention, the disclosures of which are incorporated herein by reference) describe the extraction of oilseeds (including those containing such Method for separating protein isolates from canola seeds) meal. The steps involved in this method include solubilizing protein material from the oilseed meal (alo...

Claims

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Application Information

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IPC IPC(8): A23L1/305A23J1/14A23J3/14
CPCA23J1/14A23V2002/00A23J3/14A23V2300/14A23V2300/34
Inventor L·D·巴克R·W·马滕斯E·D·默里
Owner BURCON NUTRASCI MB
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