213 results about "Protein concentration" patented technology

Provided are an apolipoprotein E detection reagent and the use thereof. The reagent comprises at least one of an ApoE2 protein detection reagent, an ApoE3 protein detection reagent and an ApoE4 protein detection reagent. One of a capture antibody and a detection antibody used for detecting ApoE2 protein or ApoE4 protein is a specific monoclonal antibody, and the capture antibody and the detection antibody used for detecting ApoE3 are both specific monoclonal antibodies. Genotyping of ApoE and quantitative detection of different genotypes of proteins are performed on the basis of an immunological detection method. The detection method has the advantages of being simple, easy to operate, short in time and cheap. A genotype detection result is highly consistent with a fluorescence PCR result. Genotyping of ApoE (6 types) can be performed, which is used to replace the nucleic acid detection and guide medication, and clarify the correlation of the ApoE4 genotype homozygosity / heterozygosity and the protein concentration of ApoE4 with AD.

The invention provides an anorectal surgery postoperative care rehabilitation evaluation method and system. Wherein a vibration sensor is constructed through a machine tool machining vibration monitoring technology, vibration frequency offset of the dressing after absorbing wound seepage is detected in real time, and the vibration frequency offset is associated with dressing weight variation; meanwhile, an optical scattering measurement technology is adopted for carrying out scattering spectrum analysis on wound exudate, and the protein concentration index is analyzed. And carrying out time sequence correlation on the weight change data corresponding to the vibration frequency offset and the protein concentration index to generate a seepage component dynamic change curve. And through a wireless transmission unit in the remote measurement system, performing remote synchronous comparison on the dynamic change curve and a preset healing stage reference curve, and when the vibration frequency offset exceeding the standard seepage amount continuously appears in the curve and the protein concentration index breaks through the threshold range, triggering a remote early warning signal of wound healing abnormity. According to the technical scheme provided by the invention, the nursing rehabilitation efficiency and precision are improved.

A method for the manufacture of an oat peptide isolate, the oat peptide isolate comprising at least 80% w / w peptides, and less than 0.1% w / w total soluble fibre. The method comprises combining an oat protein concentrate having a protein concentration of at least 40% w / w with an enzyme capable of facilitating carbohydrate hydrolysis, removing the sugar fraction, and combining with an enzyme capable of peptide formation.

The invention belongs to the technical field of biomedicine, and particularly relates to a paris polyphylla exosome, a preparation method thereof and application of the paris polyphylla exosome in treatment of psoriasis, in the method, fresh paris polyphylla rhizomes are cleaned and washed with a buffering agent, the washed paris polyphylla rhizomes and the buffering agent are mixed according to the mass ratio of 1: (1.5-2.5), after mixing, crushing and filtering are conducted, and the paris polyphylla exosome is obtained; the preparation method comprises the following steps: extracting paris polyphylla from paris polyphylla to obtain a paris polyphylla crude extract for later use, carrying out differential centrifugation on the paris polyphylla crude extract, taking supernate and concentrating to obtain an exosome crude extract, sequentially adding the exosome crude extract into cane sugar with the concentration of 15%, 30%, 45% and 60%, and carrying out cane sugar gradient purification to obtain the paris polyphylla exosome. The paris polyphylla exosome is in the shape of a cup-shaped circle and has a complete double-layer membrane structure, the protein concentration is 3.5-4.5 ug / ml, the particle size is 100-200 nm, and the paris polyphylla exosome is beneficial to improvement of psoriasis, is good in curative effect and fast in effect taking, and has high economical efficiency and safety.

The invention discloses an intelligent pulmonary tuberculosis detection method and system based on images and clinical data, and belongs to the field of medicines.The method comprises the steps that S1, high-resolution chest X-ray images and clinical data such as serum C-reactive protein concentration, erythrocyte sedimentation value, erythrocyte distribution width and body temperature are collected; s2, performing non-linear contrast enhancement on the image, and extracting lung gate texture, lung field transparency and cavity edge features; s3, fusing the image and the clinical features through a graph attention mechanism, and generating a high-dimensional fusion vector; s4, risk grade discrimination is carried out through a multi-scale residual mapping network, and five-grade labels are output; and S5, optimizing the model by utilizing the pathology definite diagnosis sample in combination with the cross entropy and the center loss. The method has the beneficial effects that accurate grading evaluation of the pulmonary tuberculosis risk is realized, and the accuracy of early screening and the clinical interpretability of the model are improved.

The invention relates to the technical field of medical treatment, and particularly discloses a compound prognosis model for cancer prognosis evaluation, a prognosis evaluation method and a calculator. The compound prognosis model is constructed based on inflammation burden related prognosis indexes ALNCR and TNM by stage combination; the creation method comprises the following steps: step 1, collecting albumin concentration, lymphocyte count, neutrophil count and C-reactive protein concentration data of a cancer patient; 2, calculating an ALNCR index according to a formula; 3, according to a clinical classic TNM staging system, the patients are divided into a TNM staging stage I, a TNM staging stage II, a TNM staging stage III or a TNM staging stage IV; 4, the ALNCR index obtained in the step 2 and the TNM staging result obtained in the step 3 are associated and integrated, and the individualized prognosis score of the ALNCR.TNM composite prognosis model is obtained through calculation. The method is suitable for clinical rapid application; high-risk patients can be accurately distinguished, a quantitative basis is provided for individualized treatment and follow-up visit strategy formulation, and excessive medical treatment or insufficient treatment is reduced.

The invention provides an anti-freezing denaturation agent for golden and tiger hybrid spotted fish meat protein and a preparation method of the anti-freezing denaturation agent. The invention relates to an anti-freezing denaturation agent for protein of Jinhu hybrid spotted fish. The anti-freezing denaturation agent is prepared from the following raw materials in parts by weight: 5 to 8 parts of trehalose-mannitol, 0.5 to 1 part of nano chitosan, 0.2 to 0.5 part of rosmarinic acid and 0.1 to 0.2 part of polysorbate-80. The trehalose-mannitol is prepared from trehalose and mannitol. Aiming at the characteristics of Jinhu hybrid spotted fish meat, trehalose-mannitol, nano chitosan, rosmarinic acid and polysorbate-80 are taken as raw materials and compounded to obtain a new formula of the protein anti-freezing denaturation agent for the Jinhu hybrid spotted fish meat, and the anti-freezing denaturation agent prepared by adopting the formula and the process disclosed by the invention can be used for inhibiting protein degradation and improving the anti-freezing denaturation effect of the Jinhu hybrid spotted fish meat. The decrease range of MP protein concentration, MP total sulfydryl content and MP Ca < 2 + >-ATPase activity of the antifreeze agent is obviously reduced, and the effect of the antifreeze agent is superior to that of a commercially available antifreeze agent. Moreover, the anti-freezing denaturation agent for the protein of the Jinhu hybrid spotted fish does not contain phosphorus, and has the advantages of safety and high efficiency.

The invention relates to a drug target determination method and device, computer equipment and a storage medium. The method comprises the following steps: obtaining a protein heat map corresponding to candidate proteins; based on a feature extraction model, extracting features of the protein heat map to obtain heat map features corresponding to the candidate proteins; based on the heat map features corresponding to the candidate proteins, clustering the candidate proteins to obtain a plurality of candidate protein sets; based on the temperature data and the protein concentration data of each candidate protein in a candidate protein set, determining the protein concentration difference significance corresponding to the candidate protein set, and based on the protein concentration difference significance and the protein concentration data of the candidate proteins, determining the protein concentration difference significance corresponding to the candidate protein set; and determining standard reference data of the candidate proteins, and determining a drug target based on the standard reference data of each candidate protein in the plurality of candidate protein sets. According to the embodiment of the invention, the drug target can be accurately determined.

The invention discloses a method suitable for extracting extracellular small vesicles in fresh water pearl shell tissue waste liquid, which comprises the following operation steps: (1) solid-liquid separation: slaughtering pearl shells, and filling slaughtering waste liquid into a waste liquid crude extraction device; (2) waste liquid crude extraction: performing multi-stage gradient filtration and gravity settling on the slaughtering waste liquid by using a waste liquid crude extraction device to obtain clarified waste liquid; (3) removing impurities: removing impurities for the first time and removing impurities for the second time; (4) concentration of the extracellular vesicles: concentrating the extracellular vesicles by using an ultra-speed centrifuge to obtain a high-concentration extracellular vesicle solution; and (5) preservation of extracellular vesicles: according to the final volume of the high-concentration extracellular vesicle solution obtained in the step (4), carrying out sample standardization through protein concentration, sub-packaging the sample, and carrying out long-term preservation in a low-temperature environment. The particle sizes of the extracted vesicles are concentrated, high-purity and complete extracellular small vesicles can be obtained, and the method is suitable for biomedical research and product development.

The invention relates to the technical field of sample analysis, and discloses a specific protein detection method based on double-angle scattering turbidimetry, which comprises the following steps: S1, obtaining a small-angle signal concentration relation curve and a medium-angle signal concentration relation curve, and obtaining a cross threshold signal; s2, the laser light source irradiates the reaction cup from one side of the reaction cup along the horizontal axis, the detection module is located on the other side of the reaction cup and opposite to the laser light source, and the detection module collects small-angle and medium-angle scattering signals at the same time and transmits collected scattering signal information to the control module; and S3, the control module compares the received medium-angle scattering signal information with the cross threshold signal information, dynamically selects and adopts a small-angle scattering signal, and obtains the concentration of the specific protein to be detected according to a small-angle signal concentration relation formula. Or obtaining the concentration of the specific protein to be detected by adopting the medium-angle scattering signal according to a medium-angle signal concentration relation formula. The method can improve the accuracy of protein concentration analysis.

The invention relates to the technical field of pathogen detection, in particular to a rapid pathogen detection system based on nanopore sequencing, which comprises an acquisition and pretreatment module, which is used for precipitating and centrifuging serum, carrying out layered monitoring, carrying out supernate separation and concentration, and measuring pH value and temperature. According to the invention, the serum is precipitated and centrifuged, and layered monitoring is combined, so that the layered state of the serum sample in the centrifugation process is visualized, and the stability of precipitated particles and the separation precision of supernate are improved. And in the supernatant concentration link, the dynamic monitoring and optimization of the sample purity are realized by measuring the pH value and the temperature and combining the recording of the protein concentration and the turbidity value. Absorbance and viscosity measurement are combined, so that the change of liquid properties can be captured in real time and compared with a pathogen directory range threshold value, and an abnormal sample can be recognized in an early stage.

The invention discloses a microneedle array electrochemical sensor for monitoring a sepsis marker C-reactive protein in skin interstitial fluid. According to the novel-arrangement cactus-like structure microneedle array electrode, the enrichment efficiency of detection liquid is remarkably improved through the design that the structure sequentially extends from the center to the periphery, and good attachment to skin tissue is achieved in combination with a flexible substrate material. Electrode interface construction of the electrochemical sensor is based on electrochemical deposition, a C-reactive protein aptamer is taken as a recognition object, a highly uniform nucleic acid C-reactive protein aptamer probe single layer is formed by combining a freezing-unfreezing method self-assembly technology, and the nucleic acid C-reactive protein aptamer probe single layer has a good linear relationship with a detection electric signal in a C-reactive protein concentration range of 0.5-100 ng / mL; the kit has the properties of high sensitivity, high specificity, rapid response, real-time monitoring, minimally invasive property and the like, is beneficial to rapid detection of C-reactive protein and dynamic monitoring of sepsis course change, and also provides technical support for development of biosensors for biomarker detection in complex media.

The invention provides a detection system for detecting influenza B virus by a one-tube method, and also provides a corresponding kit and a method for detecting influenza B virus. According to the invention, a one-step detection system is established by optimizing reaction temperature and Cas protein concentration and balancing RPA amplification and CRISPR / Cas cis-cleavage reaction. The detection system provided by the invention can specifically recognize the influenza B virus within 45 minutes, does not need uncovering operation, and avoids cross contamination. The detection limit (LoD) of the detection system is 58 copy / test, and no cross reaction exists. The one-tube method detection system and the one-step method detection system have high specificity (98.21% and 98.67%) on detection of the influenza B virus, and have high consistency (96.74% and 96.38%) in clinical samples.

The invention discloses a liquid milk UHT cleaning model construction method based on multiple monitoring parameters, and the method comprises the steps: setting cleaning parameters, carrying out the multi-parameter monitoring through a plurality of monitoring probes in a CIP cleaning process, and carrying out the sampling at preset time intervals, so as to measure the protein concentration; by adopting a response surface analysis method, analyzing and fitting monitoring data, sampling measurement values and cleaning parameters of each probe to obtain an equation model of the cleaning time to the cleaning temperature, the cleaning flow speed and the cleaning liquid concentration; a symbolic regression method is adopted, and an equation model of the cleaning speed to the cleaning time, the cleaning temperature, the cleaning flow speed and the cleaning liquid concentration is obtained by analyzing and fitting the monitoring data, the sampling measurement values and the cleaning parameters of all the probes. The cleaning time equation and the cleaning rate equation adopt multiple parameters to carry out modeling research on the cleaning process, and reference is provided for optimization of the pipeline cleaning process in the dairy industry.

An ex vivo test system 10 is constituted of a fluid circuit that circulates a prescribed circulation liquid by simulating the blood circulation state of a human body, and comprises a circulation liquid maintenance unit 17 that maintains, in the same manner as the blood environment in vivo, chemical factors in the circulation liquid, which affect the performance evaluation of a test object S. The circulation liquid maintenance unit 17 comprises: a replenishing liquid unit 23 that maintains the chloride ion concentration and protein concentration of the circulation liquid at desired values by injecting a replenishing liquid composed of an electrolyte-free isotonic solution into the fluid circuit; and a carbon dioxide supply unit 24 that maintains the pH of the circulation liquid at a desired value by supplying carbon dioxide into the fluid circuit, the state of the circulation liquid being maintained while compensating for the effect of moisture loss during circulation of the circulation liquid.

The present application mainly relates to a kind of preparation of sulfur-rich amino acid protein CMd-copper nanoclusters and its detection mercury ion method, comprising: a) by prokaryotic expression obtains CMd protein, and the protein concentration of 5 mg / mL-25 mg / mL CMd protein solution is obtained by purification.b) CMd is reacted with reducing agent TCEP after opening protein disulfide bond.c) the protein solution after reduction is reacted with anhydrous copper sulfate, and preparation high fluorescence CMd-copper nanocluster.d) CMd-copper nanocluster is applied to detect heavy metal mercury ion.The application method can be synthesized under mild aqueous conditions by "one-step method" structure controllable, stable CMd-copper nanocluster, widens the raw material of copper nanocluster synthesized with protein as template, avoids the use of organic solvent and strong reducing agent, and reduces the cost and environmental burden brought by noble metal raw material, and also has higher selectivity to mercury ion.

1. The name of the design product: integrated ultrafiltration tube. 2. The use of the design product: for protein concentration, virus, nanometer material filtration, solution separation. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The invention provides a portable stable urine protein qualitative detection kit, and relates to the technical field of urine protein qualitative detection. A homogenizing tank is fixedly mounted at the front end in the kit body, a conveying pipe is connected to the middle in the homogenizing tank in a penetrating manner, through holes in an annular array are formed in the surface of the position, located in the homogenizing tank, of the conveying pipe, and a limiting sleeve is fixedly connected to the top of the conveying pipe. A uniform tank is arranged in the kit, a bevel gear and an annular gear are driven by a motor, and a vertical plate at the bottom of the annular gear is driven to rotate in the uniform tank, so that a liquid sample can be fully and uniformly stirred, protein in the sample can be more uniformly distributed, and a detection result can more accurately reflect the actual content of protein in urine; and the detection reliability is improved. The problems that a kit is lack of an effective sample uniform treatment mechanism, if a urine sample is not fully uniform before detection, the protein concentration of a detection part is inconsistent, and the detection result has deviation are solved.

The present invention has been made to solve the above-described problems of conventional MP whey, and an object of the present invention is to provide a protein material containing MP whey, which can be prepared by a simple method, does not contain extra additives, and has a wide range of applications, and a method for producing the protein material.SOLUTION: The present invention provides a method for producing a protein material containing MP whey, the method including: (1) a step of preparing a whey solution having a protein concentration of 11 mass% or less and a solid content concentration of 19 mass% or less; and (2) a step of shearing the whey solution prepared in the step (1) at 70 °C or higher and 95 °C or lower. The present invention also provides a protein material containing MP whey, wherein the MP whey has a median diameter of 2 μm or more and 9 μm or less, and the ratio of the mass of the MP whey protein to the mass of all proteins is 40% or more and 85% or less.SELECTED DRAWING: None

The application discloses a preparation method of heat-resistant and anti-aggregation egg white protein microgel particles, which comprises the following steps: 1) adjusting the pH of separated egg white to neutral by acid water, stirring, centrifuging, and collecting supernatant; 2) diluting the collected supernatant to an egg white protein solution with a protein concentration of 1-5% by water, then adding an anionic polysaccharide solution with a concentration of 1-3%, and heating at 70-100 DEG C for 10-60 minutes to realize phase separation, and obtaining an egg white protein microgel suspension after cooling; and 3) centrifuging the microgel suspension and cleaning with water to obtain egg white protein microgel particles. The egg white protein particles are prepared by using the heat treatment-protein polysaccharide phase separation method, which is beneficial to preventing the re-aggregation of the egg white protein particles under high-temperature conditions, and has a better application prospect in the preparation of egg white protein beverages.

The invention discloses a blocking agent for cell immunofluorescence detection by a slide method and a use method of the blocking agent. The blocking agent is a homologous no-load cell lysis solution without transfection plasmids, and the protein concentration is greater than or equal to 30mg / mL. The blocking agent is completely the same as cell cytoplasm and cell membrane components of cells to be detected except for plasmids-expressed antigens, when in use, the blocking agent is pre-mixed with a blood cleaning sample in advance, interfering antibodies except target antibodies in serum can be purified, and meanwhile, due to the fact that no antigen protein expressed after plasmid transfection exists, the blocking agent can be used for detecting the cells to be detected. And the protein concentration is equivalent to the globulin concentration in serum, so that the target antibody cannot be lost in the serum, and the slide method CBA detection sensitivity can be greatly improved.

The invention discloses a full-value protein biological feed production method, and relates to the technical field of protein feed production, the production method comprises the following steps: double-strain co-fermentation: cooling fermentable syrup to 38-42 DEG C, adjusting the pH value to 6.5-7.0, inoculating a mixed strain of Thermoactinomyces vulgaris and Candida utilis, fermenting for 18-24 hours under the condition that the dissolved oxygen is maintained to be 30-60%, and carrying out double-strain co-fermentation; in the process, the pH, dissolved oxygen and temperature are monitored in real time by an on-line sensor controlled by a PLC (Programmable Logic Controller), and materials are automatically supplemented; and membrane separation and concentration: enabling the fermentation liquor to pass through a PVDF (Polyvinylidene Fluoride) ultrafiltration membrane with the molecular weight cutoff of 10kDa-30kDa, and intercepting thalli and protein aggregates by a membrane concentration multiple of 5-10 to obtain a membrane concentrated solution with the protein concentration of more than or equal to 50g / L. According to the method, agricultural waste is efficiently converted into complete protein through double-bacterium synergistic fermentation, the problems that traditional single-bacterium fermentation is low in yield and unbalanced in nutrition are solved, and the protein yield is increased.

Embodiments of the present disclosure describe a system and method for controlling the pH of a polypeptide-containing mixture. The pH may be controlled for any suitable purpose, for example, to inactivate a virus in the mixture. The method may include the step of eluting a mixture (e.g., an eluent) having a pH greater than, for example, 3.9 and, for example, less than, 8.5 from a chromatography column. The method may further include one or more of the steps of measuring the protein concentration of the mixture and measuring the pH of the mixture. Subsequently, the amount of acid required to reduce the pH of the mixture to a target pH may be calculated based on the protein concentration of the mixture, the pH of the mixture, or both. After calculating the amount of acid to be added, a portion of the acid may be added to the mixture, wherein said portion of the acid is sufficient to achieve the target pH.

The utility model relates to the field of culture solution concentration detection, in particular to an on-line protein concentration detection device for expanding a concentration range. The device comprises a first injection pump, a selector valve, a container assembly, a dilution and detection assembly and an air filter, the selector valve is provided with a public port and six selection ports uniformly distributed around the public port, and the public port is communicated with one of the six selection ports; a buffer ring is communicated between the first injection pump and the common port of the selection valve; the container assembly comprises a calibration standard liquid container, a first cleaning liquid container, a second cleaning liquid container and a culture container; the dilution and detection assembly comprises a three-way valve, a dilution assembly and a detection assembly respectively communicated with two ends of the three-way valve, and a sample reserving valve communicated with the output end of the detection assembly. According to the utility model, the detection concentration range is expanded to 6.5 orders of magnitude, the requirement of the protein measurement concentration range from extremely low concentration to extremely high concentration in the whole cell culture process is met, and the measurement accuracy and sensitivity are high.

To provide methods and apparatus for simultaneously detecting a quantity and / or concentration within a large range of protein.SOLUTION: Some embodiments described herein relate to a method that includes separating an analyte-containing sample via electrophoresis in a capillary. The capillary is loaded with a chemiluminescence agent, such as luminol, that is configured to react with the analyte (e.g., HRP-conjugated proteins) to produce a signal indicative of a concentration and / or quantity of analyte at each location along the length of the capillary. A first image of the capillary containing the analytes and the chemiluminescence agent is captured over a first period of time. A second image of the capillary containing the analytes and the chemiluminescence agent is captured over a second, longer, period of time. A concentration and / or quantity of a first population of analytes at a first location is determined using the first image, and a concentration and / or quantity of a second population of analytes at a second location is determined using the second image.SELECTED DRAWING: None

The present invention provides an siRNA that inhibits angiopoietin-like 4 (ANGPTL4) gene expression, wherein the siRNA comprises a sense strand and an antisense strand, wherein the antisense strand comprises at least 17 continuous nucleotides that differ by no more than 4 nucleotides from the nucleotide sequences as shown in SEQ ID NO: 143, and the antisense strand is 17 to 30 nucleotides in length; the sense strand is 17 to 30 nucleotides in length and is at least partially complementary to the antisense strand. The siRNA, siRNA conjugate, and pharmaceutical composition provided in the present invention exhibit good stability, ANGPLT4 gene inhibitory activity, and immunostimulation, and can significantly reduce ANGPTL4 protein concentrations at the animal level.

The application provides a Pickering emulsion with slow-release antibacterial function and a preparation method and application thereof, and relates to the technical field of Pickering emulsions. Raw materials of the Pickering emulsion include royal jelly extract liquid and yizhi essential oil in a volume ratio of 1.5-2.5:1, and the volume fraction of the yizhi essential oil in the Pickering emulsion is 60%-70%, the royal jelly extract liquid is obtained by dispersing royal jelly protein extract in water, and the obtained dispersion liquid has a protein concentration of 40-50 mg / mL. The Pickering emulsion takes the royal jelly protein extract as a natural particle stabilizer, and loads the yizhi essential oil, and through accurate control of the volume fraction of the yizhi essential oil and the protein concentration in the royal jelly extract liquid, excellent antibacterial, antioxidant activity and volatile component slow-release characteristics are exhibited. The Pickering emulsion can not only be used as a nutritionally fortified 3D printing food base material for patients with difficulty in swallowing, but also be used as an active additive for pharmaceutical preparations and cosmetics, and has wide application value.

The invention relates to a method for determining enzyme activity of ODC1 (ornithine decarboxylase 1), which comprises the following steps: digesting, centrifuging and resuspending cells, and taking a part of sample to detect protein concentration; the method comprises the following steps: cracking cells by using a specially-made enzyme activity cell lysis solution and a protein lysis solution, and ensuring full cracking through ultrasonication; carrying out quantitative analysis on the protein in the lysate by adopting a BCA kit; in the enzyme activity detection stage, a cell lysis solution and an enzyme activity detection working solution containing a specific fluorescent probe (CB6) are mixed and react at 37 DEG C for a certain time, and then the change of fluorescence intensity is determined by a multifunctional fluorescence microplate reader, so that the enzyme activity of the ODC1 is evaluated. According to the method, accurate determination of the ODC1 enzyme activity is realized by optimizing cell treatment, protein quantification and enzyme activity detection processes. The method has the advantages of simplicity and convenience in operation, high sensitivity, good specificity and the like, and is suitable for the fields of basic research, drug screening and the like related to the ODC1 enzyme activity.

The present invention provides a Pichia pastoris genetically engineered bacterium expressing human lysozyme and its application. The Pichia pastoris genetically engineered bacterium Δ0823-CH2 is classified as Komagataella phaffii and has a deposit number of CGMCC NO.28602. A high-yield mutant strain was obtained by co-expressing the PDI1 gene and selecting and breeding by atmospheric pressure and room temperature plasma mutagenesis. The PAS_chr3_0823 gene was knocked out by genetic engineering technology on the basis of the strain. The strain was fermented at high density in a 5L fermentor, and the human lysozyme activity in the fermentation supernatant was as high as 1,032,667±11,719U / mL, and the protein concentration reached 4.10±0.01g / L, which is the highest level currently. The Pichia pastoris engineered bacterium of the present invention can express human lysozyme with high enzyme activity and high protein concentration, and has a wide range of industrial application value in the fields of food, feed, etc.

The invention relates to a preparation method of a fish gelatin edible film, which comprises the following steps: S1, adding glycerol into a fish gelatin solution, uniformly mixing, and defoaming to obtain a film forming solution; s2, injecting the film-forming liquid into an organic silicon resin mold, and performing primary drying at the temperature of not higher than 30 DEG C to obtain a concentrated film-forming liquid with the protein concentration of not lower than 20%; and S3, carrying out secondary drying on the concentrated film-forming liquid at a temperature not lower than 90 DEG C, cooling to room temperature, uncovering the film, and balancing to obtain the fish gelatin edible film. According to the invention, the film-forming liquid is subjected to primary drying at the temperature of less than or equal to 30 DEG C until the high protein concentration is more than or equal to 20%, and the interaction force among gelatin molecules is enhanced by improving the protein concentration, so that the stability of a triple-helical structure is facilitated, and the structural integrity can still be kept in the high-temperature secondary drying process; the high-quality fish gelatin edible film with the performance comparable with that of a low-temperature drying process is efficiently prepared, and the technical problem that the performance of the gelatin film is degraded due to high-temperature drying is solved.