145 results about "Protein concentration" patented technology

A method for the manufacture of an oat peptide isolate, the oat peptide isolate comprising at least 80% w / w peptides, and less than 0.1% w / w total soluble fibre. The method comprises combining an oat protein concentrate having a protein concentration of at least 40% w / w with an enzyme capable of facilitating carbohydrate hydrolysis, removing the sugar fraction, and combining with an enzyme capable of peptide formation.

The invention relates to the technical field of medical treatment, and particularly discloses a compound prognosis model for cancer prognosis evaluation, a prognosis evaluation method and a calculator. The compound prognosis model is constructed based on inflammation burden related prognosis indexes ALNCR and TNM by stage combination; the creation method comprises the following steps: step 1, collecting albumin concentration, lymphocyte count, neutrophil count and C-reactive protein concentration data of a cancer patient; 2, calculating an ALNCR index according to a formula; 3, according to a clinical classic TNM staging system, the patients are divided into a TNM staging stage I, a TNM staging stage II, a TNM staging stage III or a TNM staging stage IV; 4, the ALNCR index obtained in the step 2 and the TNM staging result obtained in the step 3 are associated and integrated, and the individualized prognosis score of the ALNCR.TNM composite prognosis model is obtained through calculation. The method is suitable for clinical rapid application; high-risk patients can be accurately distinguished, a quantitative basis is provided for individualized treatment and follow-up visit strategy formulation, and excessive medical treatment or insufficient treatment is reduced.

The invention discloses a method suitable for extracting extracellular small vesicles in fresh water pearl shell tissue waste liquid, which comprises the following operation steps: (1) solid-liquid separation: slaughtering pearl shells, and filling slaughtering waste liquid into a waste liquid crude extraction device; (2) waste liquid crude extraction: performing multi-stage gradient filtration and gravity settling on the slaughtering waste liquid by using a waste liquid crude extraction device to obtain clarified waste liquid; (3) removing impurities: removing impurities for the first time and removing impurities for the second time; (4) concentration of the extracellular vesicles: concentrating the extracellular vesicles by using an ultra-speed centrifuge to obtain a high-concentration extracellular vesicle solution; and (5) preservation of extracellular vesicles: according to the final volume of the high-concentration extracellular vesicle solution obtained in the step (4), carrying out sample standardization through protein concentration, sub-packaging the sample, and carrying out long-term preservation in a low-temperature environment. The particle sizes of the extracted vesicles are concentrated, high-purity and complete extracellular small vesicles can be obtained, and the method is suitable for biomedical research and product development.

The invention relates to the technical field of sample analysis, and discloses a specific protein detection method based on double-angle scattering turbidimetry, which comprises the following steps: S1, obtaining a small-angle signal concentration relation curve and a medium-angle signal concentration relation curve, and obtaining a cross threshold signal; s2, the laser light source irradiates the reaction cup from one side of the reaction cup along the horizontal axis, the detection module is located on the other side of the reaction cup and opposite to the laser light source, and the detection module collects small-angle and medium-angle scattering signals at the same time and transmits collected scattering signal information to the control module; and S3, the control module compares the received medium-angle scattering signal information with the cross threshold signal information, dynamically selects and adopts a small-angle scattering signal, and obtains the concentration of the specific protein to be detected according to a small-angle signal concentration relation formula. Or obtaining the concentration of the specific protein to be detected by adopting the medium-angle scattering signal according to a medium-angle signal concentration relation formula. The method can improve the accuracy of protein concentration analysis.

The invention discloses a liquid milk UHT cleaning model construction method based on multiple monitoring parameters, and the method comprises the steps: setting cleaning parameters, carrying out the multi-parameter monitoring through a plurality of monitoring probes in a CIP cleaning process, and carrying out the sampling at preset time intervals, so as to measure the protein concentration; by adopting a response surface analysis method, analyzing and fitting monitoring data, sampling measurement values and cleaning parameters of each probe to obtain an equation model of the cleaning time to the cleaning temperature, the cleaning flow speed and the cleaning liquid concentration; a symbolic regression method is adopted, and an equation model of the cleaning speed to the cleaning time, the cleaning temperature, the cleaning flow speed and the cleaning liquid concentration is obtained by analyzing and fitting the monitoring data, the sampling measurement values and the cleaning parameters of all the probes. The cleaning time equation and the cleaning rate equation adopt multiple parameters to carry out modeling research on the cleaning process, and reference is provided for optimization of the pipeline cleaning process in the dairy industry.

An ex vivo test system 10 is constituted of a fluid circuit that circulates a prescribed circulation liquid by simulating the blood circulation state of a human body, and comprises a circulation liquid maintenance unit 17 that maintains, in the same manner as the blood environment in vivo, chemical factors in the circulation liquid, which affect the performance evaluation of a test object S. The circulation liquid maintenance unit 17 comprises: a replenishing liquid unit 23 that maintains the chloride ion concentration and protein concentration of the circulation liquid at desired values by injecting a replenishing liquid composed of an electrolyte-free isotonic solution into the fluid circuit; and a carbon dioxide supply unit 24 that maintains the pH of the circulation liquid at a desired value by supplying carbon dioxide into the fluid circuit, the state of the circulation liquid being maintained while compensating for the effect of moisture loss during circulation of the circulation liquid.

The present application mainly relates to a kind of preparation of sulfur-rich amino acid protein CMd-copper nanoclusters and its detection mercury ion method, comprising: a) by prokaryotic expression obtains CMd protein, and the protein concentration of 5 mg / mL-25 mg / mL CMd protein solution is obtained by purification.b) CMd is reacted with reducing agent TCEP after opening protein disulfide bond.c) the protein solution after reduction is reacted with anhydrous copper sulfate, and preparation high fluorescence CMd-copper nanocluster.d) CMd-copper nanocluster is applied to detect heavy metal mercury ion.The application method can be synthesized under mild aqueous conditions by "one-step method" structure controllable, stable CMd-copper nanocluster, widens the raw material of copper nanocluster synthesized with protein as template, avoids the use of organic solvent and strong reducing agent, and reduces the cost and environmental burden brought by noble metal raw material, and also has higher selectivity to mercury ion.

1. The name of the design product: integrated ultrafiltration tube. 2. The use of the design product: for protein concentration, virus, nanometer material filtration, solution separation. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The invention provides a portable stable urine protein qualitative detection kit, and relates to the technical field of urine protein qualitative detection. A homogenizing tank is fixedly mounted at the front end in the kit body, a conveying pipe is connected to the middle in the homogenizing tank in a penetrating manner, through holes in an annular array are formed in the surface of the position, located in the homogenizing tank, of the conveying pipe, and a limiting sleeve is fixedly connected to the top of the conveying pipe. A uniform tank is arranged in the kit, a bevel gear and an annular gear are driven by a motor, and a vertical plate at the bottom of the annular gear is driven to rotate in the uniform tank, so that a liquid sample can be fully and uniformly stirred, protein in the sample can be more uniformly distributed, and a detection result can more accurately reflect the actual content of protein in urine; and the detection reliability is improved. The problems that a kit is lack of an effective sample uniform treatment mechanism, if a urine sample is not fully uniform before detection, the protein concentration of a detection part is inconsistent, and the detection result has deviation are solved.

The present invention has been made to solve the above-described problems of conventional MP whey, and an object of the present invention is to provide a protein material containing MP whey, which can be prepared by a simple method, does not contain extra additives, and has a wide range of applications, and a method for producing the protein material.SOLUTION: The present invention provides a method for producing a protein material containing MP whey, the method including: (1) a step of preparing a whey solution having a protein concentration of 11 mass% or less and a solid content concentration of 19 mass% or less; and (2) a step of shearing the whey solution prepared in the step (1) at 70 °C or higher and 95 °C or lower. The present invention also provides a protein material containing MP whey, wherein the MP whey has a median diameter of 2 μm or more and 9 μm or less, and the ratio of the mass of the MP whey protein to the mass of all proteins is 40% or more and 85% or less.SELECTED DRAWING: None

The application discloses a preparation method of heat-resistant and anti-aggregation egg white protein microgel particles, which comprises the following steps: 1) adjusting the pH of separated egg white to neutral by acid water, stirring, centrifuging, and collecting supernatant; 2) diluting the collected supernatant to an egg white protein solution with a protein concentration of 1-5% by water, then adding an anionic polysaccharide solution with a concentration of 1-3%, and heating at 70-100 DEG C for 10-60 minutes to realize phase separation, and obtaining an egg white protein microgel suspension after cooling; and 3) centrifuging the microgel suspension and cleaning with water to obtain egg white protein microgel particles. The egg white protein particles are prepared by using the heat treatment-protein polysaccharide phase separation method, which is beneficial to preventing the re-aggregation of the egg white protein particles under high-temperature conditions, and has a better application prospect in the preparation of egg white protein beverages.

The invention discloses a blocking agent for cell immunofluorescence detection by a slide method and a use method of the blocking agent. The blocking agent is a homologous no-load cell lysis solution without transfection plasmids, and the protein concentration is greater than or equal to 30mg / mL. The blocking agent is completely the same as cell cytoplasm and cell membrane components of cells to be detected except for plasmids-expressed antigens, when in use, the blocking agent is pre-mixed with a blood cleaning sample in advance, interfering antibodies except target antibodies in serum can be purified, and meanwhile, due to the fact that no antigen protein expressed after plasmid transfection exists, the blocking agent can be used for detecting the cells to be detected. And the protein concentration is equivalent to the globulin concentration in serum, so that the target antibody cannot be lost in the serum, and the slide method CBA detection sensitivity can be greatly improved.

The invention discloses a full-value protein biological feed production method, and relates to the technical field of protein feed production, the production method comprises the following steps: double-strain co-fermentation: cooling fermentable syrup to 38-42 DEG C, adjusting the pH value to 6.5-7.0, inoculating a mixed strain of Thermoactinomyces vulgaris and Candida utilis, fermenting for 18-24 hours under the condition that the dissolved oxygen is maintained to be 30-60%, and carrying out double-strain co-fermentation; in the process, the pH, dissolved oxygen and temperature are monitored in real time by an on-line sensor controlled by a PLC (Programmable Logic Controller), and materials are automatically supplemented; and membrane separation and concentration: enabling the fermentation liquor to pass through a PVDF (Polyvinylidene Fluoride) ultrafiltration membrane with the molecular weight cutoff of 10kDa-30kDa, and intercepting thalli and protein aggregates by a membrane concentration multiple of 5-10 to obtain a membrane concentrated solution with the protein concentration of more than or equal to 50g / L. According to the method, agricultural waste is efficiently converted into complete protein through double-bacterium synergistic fermentation, the problems that traditional single-bacterium fermentation is low in yield and unbalanced in nutrition are solved, and the protein yield is increased.

Embodiments of the present disclosure describe a system and method for controlling the pH of a polypeptide-containing mixture. The pH may be controlled for any suitable purpose, for example, to inactivate a virus in the mixture. The method may include the step of eluting a mixture (e.g., an eluent) having a pH greater than, for example, 3.9 and, for example, less than, 8.5 from a chromatography column. The method may further include one or more of the steps of measuring the protein concentration of the mixture and measuring the pH of the mixture. Subsequently, the amount of acid required to reduce the pH of the mixture to a target pH may be calculated based on the protein concentration of the mixture, the pH of the mixture, or both. After calculating the amount of acid to be added, a portion of the acid may be added to the mixture, wherein said portion of the acid is sufficient to achieve the target pH.

The utility model relates to the field of culture solution concentration detection, in particular to an on-line protein concentration detection device for expanding a concentration range. The device comprises a first injection pump, a selector valve, a container assembly, a dilution and detection assembly and an air filter, the selector valve is provided with a public port and six selection ports uniformly distributed around the public port, and the public port is communicated with one of the six selection ports; a buffer ring is communicated between the first injection pump and the common port of the selection valve; the container assembly comprises a calibration standard liquid container, a first cleaning liquid container, a second cleaning liquid container and a culture container; the dilution and detection assembly comprises a three-way valve, a dilution assembly and a detection assembly respectively communicated with two ends of the three-way valve, and a sample reserving valve communicated with the output end of the detection assembly. According to the utility model, the detection concentration range is expanded to 6.5 orders of magnitude, the requirement of the protein measurement concentration range from extremely low concentration to extremely high concentration in the whole cell culture process is met, and the measurement accuracy and sensitivity are high.

The present invention provides an siRNA that inhibits angiopoietin-like 4 (ANGPTL4) gene expression, wherein the siRNA comprises a sense strand and an antisense strand, wherein the antisense strand comprises at least 17 continuous nucleotides that differ by no more than 4 nucleotides from the nucleotide sequences as shown in SEQ ID NO: 143, and the antisense strand is 17 to 30 nucleotides in length; the sense strand is 17 to 30 nucleotides in length and is at least partially complementary to the antisense strand. The siRNA, siRNA conjugate, and pharmaceutical composition provided in the present invention exhibit good stability, ANGPLT4 gene inhibitory activity, and immunostimulation, and can significantly reduce ANGPTL4 protein concentrations at the animal level.

The application provides a Pickering emulsion with slow-release antibacterial function and a preparation method and application thereof, and relates to the technical field of Pickering emulsions. Raw materials of the Pickering emulsion include royal jelly extract liquid and yizhi essential oil in a volume ratio of 1.5-2.5:1, and the volume fraction of the yizhi essential oil in the Pickering emulsion is 60%-70%, the royal jelly extract liquid is obtained by dispersing royal jelly protein extract in water, and the obtained dispersion liquid has a protein concentration of 40-50 mg / mL. The Pickering emulsion takes the royal jelly protein extract as a natural particle stabilizer, and loads the yizhi essential oil, and through accurate control of the volume fraction of the yizhi essential oil and the protein concentration in the royal jelly extract liquid, excellent antibacterial, antioxidant activity and volatile component slow-release characteristics are exhibited. The Pickering emulsion can not only be used as a nutritionally fortified 3D printing food base material for patients with difficulty in swallowing, but also be used as an active additive for pharmaceutical preparations and cosmetics, and has wide application value.

The invention relates to a method for determining enzyme activity of ODC1 (ornithine decarboxylase 1), which comprises the following steps: digesting, centrifuging and resuspending cells, and taking a part of sample to detect protein concentration; the method comprises the following steps: cracking cells by using a specially-made enzyme activity cell lysis solution and a protein lysis solution, and ensuring full cracking through ultrasonication; carrying out quantitative analysis on the protein in the lysate by adopting a BCA kit; in the enzyme activity detection stage, a cell lysis solution and an enzyme activity detection working solution containing a specific fluorescent probe (CB6) are mixed and react at 37 DEG C for a certain time, and then the change of fluorescence intensity is determined by a multifunctional fluorescence microplate reader, so that the enzyme activity of the ODC1 is evaluated. According to the method, accurate determination of the ODC1 enzyme activity is realized by optimizing cell treatment, protein quantification and enzyme activity detection processes. The method has the advantages of simplicity and convenience in operation, high sensitivity, good specificity and the like, and is suitable for the fields of basic research, drug screening and the like related to the ODC1 enzyme activity.

The invention relates to a preparation method of a fish gelatin edible film, which comprises the following steps: S1, adding glycerol into a fish gelatin solution, uniformly mixing, and defoaming to obtain a film forming solution; s2, injecting the film-forming liquid into an organic silicon resin mold, and performing primary drying at the temperature of not higher than 30 DEG C to obtain a concentrated film-forming liquid with the protein concentration of not lower than 20%; and S3, carrying out secondary drying on the concentrated film-forming liquid at a temperature not lower than 90 DEG C, cooling to room temperature, uncovering the film, and balancing to obtain the fish gelatin edible film. According to the invention, the film-forming liquid is subjected to primary drying at the temperature of less than or equal to 30 DEG C until the high protein concentration is more than or equal to 20%, and the interaction force among gelatin molecules is enhanced by improving the protein concentration, so that the stability of a triple-helical structure is facilitated, and the structural integrity can still be kept in the high-temperature secondary drying process; the high-quality fish gelatin edible film with the performance comparable with that of a low-temperature drying process is efficiently prepared, and the technical problem that the performance of the gelatin film is degraded due to high-temperature drying is solved.

Software and hardware that can be used to perform quality control at various stages of a biopharmaceutical production process (100), e.g., during upstream or downstream processing (USP, DSP). In some examples, the disclosed Raman-based solutions enable real-time or near real-time quantification of protein concentration in various units of the biopharmaceutical production equipment, including but not limited to bioreactors, product holding vessels (105), and fluid-transfer lines. In some other examples, the disclosed Raman-based solutions enable real-time or near real-time elucidation and monitoring of the secondary structure of the protein, as a quality marker. In at least some examples, the equipment includes an electronic controller configured to perform or initiate an equipment- or process-control action based on the concentration measurements and / or evaluation of the secondary structure.

[Problem] In fields such as tumor marker test and pregnancy test kit, it is known to concentrate a target component (protein) by utilizing a superabsorbent polymer (SAP) as a method for improving test sensitivity. However, due to the powder form, SAP exhibits difficulties in handleability and formability and also causes problems such as falling off. Furthermore, the concentration effect is hardly at a sufficient level. The present invention has been made in view of such problems and on the basis of the discovery that a fiber containing an ammonium salt-type carboxyl group and a crosslinked structure serves as a material for protein concentration having excellent handleability and formability, in addition to having a high protein concentration effect. [Solution] A fiber for protein concentration is a fiber that contains an ammonium salt-type carboxyl group and a crosslinked structure, and is characterized by having a water absorption ratio of 4-300 times.

The invention belongs to the field of food science and engineering, and discloses a preparation method of an active medicinal and edible composite nutrient AMCN with an anti-aging effect, and the preparation method comprises the following steps: putting a ganoderma lucidum monoclonal strain into a liquid culture medium for controllable culture to obtain supernate with beta-glucosidase activity of over 100mU / mL; and adding rhizoma polygonati powder, lucid ganoderma powder and mulberry leaf powder, carrying out enzymatic biotransformation, carrying out lactobacillus plantarum fermentation, adding a freeze-drying protective additive, carrying out freeze-drying, grinding to obtain the active medicinal and edible composite nutrient AMCN, and storing the finished product in a dry and dark place. The active medicinal and edible composite nutrient can be used for preparing anti-aging functional foods or nutritional health-care products. A D-galactose induced zebrafish aging model verifies that the active medicinal and edible composite nutrient prepared by the application plays an anti-aging role by relieving oxidative stress, inhibiting cell aging and maintaining telomere stability, can reduce beta-galactosidase activity and intracellular ROS (reactive oxygen species) level, improves telomerase protein concentration, and has a good anti-aging effect. And the telomere length can be effectively recovered by medium and high concentrations.

The invention discloses a method for detecting the content of manganese ions in cells, and relates to the technical field of biological and medical analysis. The method comprises the following steps: performing cell lysis, preparing a standard solution, establishing a standard curve and a regression equation of manganese ion concentration and absorbance difference, and calculating the manganese ion content by measuring the absorbance difference between a sample to be detected and a blank sample and combining with protein concentration. The method is simple and convenient to operate, high in sensitivity and suitable for quantitative detection of trace manganese ions in cells, and has a good application prospect.

This invention discloses a protein detection method combining surface-enhanced Raman spectroscopy (SERS) with CRISPR / Cas12a. The method includes the following steps: incubating samples containing a series of marker proteins with aptamers; incubating the resulting products with Cas12a protein, crRNA, and a Raman probe; exciting the incubated products with a laser; detecting their Raman signals; identifying and analyzing the Raman characteristic peak of 4 ATP; and obtaining a standard curve comparing the marker protein concentration with the Raman characteristic peak intensity of 4 ATP. The same analysis is performed on samples containing unknown concentrations of marker proteins, and the marker protein concentration in the samples is calculated based on the standard curve. Compared with existing ELISA methods, this invention has higher sensitivity and higher catalytic efficiency.

The invention discloses a preparation method of egg white protein nanogel. The preparation method comprises the following steps: firstly, adding an ethanol solution with the volume fraction of 0.1-11.5% into egg white liquid, and diluting until the protein concentration is 1-2%; the egg white protein ethanol solution is subjected to microwave and ultrasonic combined treatment, the treatment time is set to be 3-7 min, ultrasonic is in an intermittent mode, the ultrasonic power is 200-800 W, and the microwave heating temperature is 65-75 DEG C. And finally, removing ethanol in the solution subjected to microwave ultrasonic treatment to obtain the egg white protein nanogel particles. The method is different from an existing'bottom-to-top 'preparation method and comprises the following steps: firstly, carrying out mild denaturation on protein by adopting low-concentration ethanol, changing a protein structure and an intermolecular acting force to expose a hydrophobic structure and expand the structure, and then moderately gathering and crushing the protein into microgel by utilizing an ultrasonic cavitation effect and a microwave heat effect; the nanogel has smaller particle size, higher system stability and interface stability, and the preparation method is simple and low in cost.

This invention discloses a method for preparing cell-derived exosome subsets, as well as products and applications, belonging to the field of biomedical technology. The preparation method includes: filtering cell culture medium to obtain a cell culture supernatant; concentrating the supernatant 5-30 times using a tangential flow system with a 300-500 kDa hollow fiber filter, followed by 10-20 times washing to obtain a concentrated solution; and performing Sephacryl S-400 HR gel filtration column chromatography, collecting the eluent from two 280 nm UV absorption peaks to obtain exosome subsets S1 and S2; the protein concentration of the concentrated solution is 20-50 mg / mL. This invention achieves, for the first time, precise separation of functional exosome subsets. Subset S1 exhibits strong immunomodulatory activity, and subset S2 demonstrates excellent cell regeneration promotion effects. Furthermore, the process is highly versatile and suitable for large-scale production. These subsets can be used to prepare immunomodulatory or cell regeneration-promoting drugs, providing precise technical solutions for the treatment of related diseases.

The invention discloses an effect and mechanism research method of GOT1 in lung adenocarcinoma cells, and relates to the technical field of biomedical research. Comprising the following steps: S1, cell culture and protein extraction: culturing Bas-2b, A549, H1573 and Calu-3 cells, cleaning the cells with PBS, performing pyrolysis treatment, collecting supernate as a total protein solution, and determining the protein concentration per tube by BCA; s2, carrying out Western Blot experiment, taking 10 micrograms of protein as a loading amount, carrying out electrophoresis, transferring a film, sealing, incubating a primary antibody and a secondary antibody, exposing and developing, and storing a picture; the experiment is repeated for multiple times, and Image J is used for analyzing the strip exposure diagram. According to the method disclosed by the invention, effective knock-down of GOT1 in A549 and H1573 cells is successfully realized through the steps of screening proper cell screening concentration by puromycin, exploring an MOI value, transfecting lentivirus, screening puromycin and the like; the invention provides an important tool and method for follow-up research on the function of GOT1 in lung adenocarcinoma cells.

A method for decomposition of keratin material, e. g. mixture of feathers, is disclosed. The method comprises treating the keratin material with Bacillus cereus KB043 strain, subsequently with sodium hydroxide and potassium hydroxide, and finally with neutralizing acid. The end product is intended for use as an amino acid fertilizer.

To provide a non-fermented high-protein milk beverage which contains protein at a high concentration yet has low viscosity, favorable flavor, and good drinkability, and a method for producing the same.SOLUTION: A method for producing a non-fermented high-protein milk beverage having a protein content of 5 mass% or more, the method comprising a step of mixing raw milk with an endo-type neutral protease, and a step of treating the obtained mixture at 15-80°C for 30-90 minutes within a neutral pH range, the beverage containing an endo-type neutral protease degradation product of the raw milk.SELECTED DRAWING: None

The invention discloses a method for improving autism behaviors through Npas4 expression and overexpression in a cat chat syndrome model, and belongs to the technical field of animal model construction. Selecting SD wild rats and CdCS model rats, and normally feeding and breeding the rats; the method comprises the following steps: measuring the protein concentration by adopting a BCA method, then carrying out Western Blot, then carrying out brain stereotactic injection, and finally carrying out behavioral testing. According to the invention, a CdCS rat model is established by using a CRISPR-Cas9 technology, and the model shows cognitive and social behavior disorders and medial prefrontal cortex (mPFC) neuron dendritic abnormality; in addition, an abnormally expressed gene is found in the model mouse, the research focuses on Npas4, and a new insight is possibly provided for disease treatment.