24 results about "Protein concentration" patented technology

Embodiments of the present disclosure describe a system and method for controlling the pH of a polypeptide-containing mixture. The pH may be controlled for any suitable purpose, for example, to inactivate a virus in the mixture. The method may include the step of eluting a mixture (e.g., an eluent) having a pH greater than, for example, 3.9 and, for example, less than, 8.5 from a chromatography column. The method may further include one or more of the steps of measuring the protein concentration of the mixture and measuring the pH of the mixture. Subsequently, the amount of acid required to reduce the pH of the mixture to a target pH may be calculated based on the protein concentration of the mixture, the pH of the mixture, or both. After calculating the amount of acid to be added, a portion of the acid may be added to the mixture, wherein said portion of the acid is sufficient to achieve the target pH.

1. The name of the design product: multifunctional enzyme label instrument. 2. The use of the design product: for nucleic acid, protein concentration, enzyme activity analysis and other conventional molecular detection; signal transduction research, cell signal event active oxygen, modification detection; cell viability, apoptosis, killing and other overall level analysis. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective drawing.

This invention discloses the application of a peptide SRD in the preparation of drugs for treating acute lung injury, belonging to the field of biomedical technology. In vivo experiments verified the protective effect of peptide SRD on acute lung injury (ALI), particularly its effect on improving AT2 mitochondrial function. In animal models of ALI, tail vein injection of peptide SRD significantly reduced the protein concentration and total cell count in bronchoalveolar lavage fluid, significantly alleviated the severity of lung injury, lowered histological scores, significantly decreased SOD2 acetylation levels while significantly increasing total SOD2 protein levels, and significantly increased SPC levels (an indicator of AT2 survival). ELISA analysis showed a significant decrease in the levels of IL-6, IL-1β, and TNF-α, indicators of pneumonia in mice. These experimental results confirm that the antioxidant peptide SRD can improve oxidative stress damage in ALI, effectively reduce AT2 cell damage under ALI conditions, promote AT2 regeneration and repair, effectively improve lung function, and reduce the inflammatory response of damaged lungs, thus exhibiting a good therapeutic effect on ALI.

The application belongs to the technical field of chemical industry, and particularly relates to a cottonseed dephenolized concentrated protein extraction equipment and method, which comprises pretreatment unit, extraction unit, post-treatment unit, evaporation and concentration unit and solvent recovery unit connected in sequence. The pretreatment unit comprises double roller embryo press, bulking machine and turnover plate cooler. The extraction unit comprises extractor A for removing oil and fat by n-hexane extraction and extractor B for removing gossypol by methanol extraction. The post-treatment unit comprises horizontal screw conveyor dryer A and horizontal screw conveyor dryer B for drying and desolventizing the extracted cottonseed meal to obtain cottonseed dephenolized concentrated protein. The evaporation and concentration unit is used for evaporating and concentrating the mixed oil and mixed liquid after extraction. The solvent recovery unit comprises multiple condensers connected with the evaporation and concentration unit. The application can realize low-temperature and high-efficiency extraction. The protein content of the extracted product cottonseed dephenolized concentrated protein is as high as 72.7-76.2%, the gossypol content is as low as 230-290 ppm, the residual solvent in the protein is as low as 280-340 ppm, and the product quality is high.

The application discloses a kind of nanoparticles for removing lung mucus and its preparation method and application, it is related to the field of biological medicine.The nanoparticles of the application are composed of inner core and shell coated on the surface of the inner core, wherein the inner core contains citric acid and photoluminescent polymer-aliphatic polyester copolymer;The shell contains deoxyribonuclease and hydrophilic polymer.The nanoparticles provided by the application have excellent biocompatibility and multifunctional integration characteristics, which realizes effective penetration of lung mucus barrier, specific degradation of DNA network, down-regulation of pro-inflammatory factors and inhibition of mucus production in sequence through cascade response mechanism, breaking through the limitations of traditional single therapy.In addition, the in vivo experimental results show that it can significantly reduce the thickness of lung mucus and the protein concentration of lavage fluid, and effectively relieve airway obstruction, and can be used for removing lung mucus.

This invention discloses a rapid detection method for protein in feed based on wet chemistry. The method includes the following steps: (1) mixing the feed sample with the sample extraction buffer, shaking to extract, centrifuging, and taking the supernatant; (2) adding the working solution of the protein standard and the supernatant to the diquinoline formic acid working solution, incubating, and measuring the absorbance value at a wavelength of 562 nm using an ELISA reader to obtain the absorbance values ​​of the protein standard and the supernatant; plotting a standard curve with the concentration of the protein standard as the abscissa and the absorbance value as the ordinate, and obtaining a linear regression equation; substituting the absorbance value of the supernatant into the linear regression equation to calculate the protein concentration in the supernatant, and then calculating the protein content in the feed sample based on the extraction buffer volume in step (1). This invention achieves a high degree of consistency between the detection results and the Kjeldahl nitrogen determination method, while significantly shortening the detection time of a single sample to less than 1 hour.

This invention discloses an ultrafiltration system with real-time protein concentration control, belonging to the field of biopharmaceutical technology. The system includes a replenishment tank, a recirculation tank, an ultrafiltration unit, and a controller. The recirculation tank is connected to the replenishment tank via a replenishment pipe, which is equipped with a replenishment pump and a valve, with the valve located between the replenishment pump and the recirculation tank. A protein concentration detection component A is located at the bottom of the recirculation tank. The ultrafiltration unit is connected to the recirculation tank via a delivery pipe and a return pipe. A circulation pump is installed on the delivery pipe, and a back pressure component and a protein concentration detection component B are installed on the return pipe. This system can improve the consistency of protein concentration in the recirculation tank during constant-volume dialysis, reduce batch-to-batch differences in protein concentration, and thus improve the uniformity of product quality. It can also prevent concentration polarization caused by excessively high protein concentration from affecting protein stability.

The present invention relates generally to preparative and purification processes relating to whey protein and particularly to the preparation and purification of Whey Protein Concentrate (WPC), also known as Whey Protein Phospholipid Concentrate (WPPC), Milk Fat Globule Membrane (MFGM), procream, Whey Fat Concentrate (WFC) and whey retentate. We describe processes for increasing a protein concentration of a composition containing milk protein and milk fat, and processes for separating milk protein and milk fats in a composition containing milk protein and milk fat. The process includes extracting the composition with a solvent selected from hexane, 2-methyloxolane, ethanol and carbon dioxide, and combinations thereof. The present invention also relates to whey protein compositions, milk fat compositions and phospholipid-rich compositions obtainable or obtained by the processes of the invention.

A composition and method of preparing a corn protein hydrolysate includes obtaining a corn protein composition having a corn protein concentration of at least about 75 wt %, adding an enzyme to a corn protein suspension containing the corn protein composition at a ratio of from about 1:100 to about 1:20 by weight of enzyme to corn protein, controlling the pH and temperature of the corn protein suspension to hydrolyze the corn protein, and terminating the hydrolysis of the corn protein to provide a corn protein hydrolysate that has solubility of from about 7% to about 37% at a pH selected from the group consisting of pH 7.0, pH 3.4, pH 5, and all of pH 7.0, pH 5, and pH 3.4. The corn protein hydrolysate can be used in a variety of food, feed, beverage, and other applications.

Systems and methods are provided for performing a titration analysis to indicate a concentration of a protein present in an experimental sample. The titration analysis system includes a titration analysis instrument having a light source, a thermally controlled sample receiver, and a light detector configured to detect light from the light source after the light has passed through the thermally controlled sample receiver. A non-transitory memory stores a reusable titration curve, and processing circuitry is configured to receive a signal from the light detector, determine a fluorescence polarization value based on the signal, retrieve the reusable titration curve from the non-transitory memory as a reference curve, and fit the fluorescence polarization value to the reusable titration curve to indicate the concentration of the protein present in the experimental sample.

The application provides a mycoplasma gallisepticum triple subunit vaccine and application thereof, and the vaccine comprises EF-TU protein with an amino acid sequence as shown in SEQ ID NO. 1, PdHD protein with an amino acid sequence as shown in SEQ ID NO. 2, MS1360 protein with an amino acid sequence as shown in SEQ ID NO. 3 and a pharmaceutically acceptable adjuvant. The application realizes antigen epitope complementation and immune synergistic enhancement by screening the optimal vaccine formula through two-by-two mixed combination and three-protein concentration gradient mixed system screening of EF-TU, PdHD and MS1360 three high immunogenic membrane proteins, and solves the problems of low protection rate and unstable immune effect of the existing vaccine.

This invention discloses a protein solution concentration device, comprising: a concentration mechanism; a pressure mechanism; and fluid pipelines. The concentration mechanism includes: a first housing and a second housing, the first housing having an inlet for the protein solution to enter the concentration mechanism and a first outlet for the concentrated solution to exit the concentration mechanism; the second housing having a second outlet for waste liquid to exit the concentration mechanism; and an ultrafiltration membrane assembly disposed within the first and second housings. One side of the ultrafiltration membrane assembly has a first flow channel connecting the inlet to the outlet, and the other side of the ultrafiltration membrane assembly has a second flow channel connecting to the second outlet. With the above configuration, the concentration mechanism has a simple and practical structure, enabling rapid and efficient protein concentration while ensuring that the protein's activity and structure are not damaged. Simultaneously, the ultrafiltration membrane assembly of this invention exhibits low protein adhesion and high flux, avoiding waste due to protein adhesion during concentration.

Embodiments of the present disclosure are directed to systems and methods for modulating pH in a mixture containing a polypeptide. The pH may be modulated for any suitable purpose, e.g., inactivating virus in the mixture. Methods may include eluting, from a chromatography column, a mixture (e.g., an eluate) having a pH greater than, e.g., 3.9 and less than, e.g., 8.5. Methods may further include one or more of measuring a protein concentration of the mixture and measuring a pH of the mixture. An amount of acid necessary to reduce the pH of the mixture to a target pH may then be calculated based on the protein concentration of the mixture, the pH of the mixture, or both. After an acid addition amount is calculated, a portion of acid may be added to the mixture, wherein the portion of acid is sufficient to achieve the target pH.

The application discloses a method for preparing salt and heat double-resistant soybean protein powder by using salt-containing preheating combined with micro-jet circulation treatment, and the method comprises the following steps: dispersing soybean protein powder in a phosphate buffer solution, preheating treatment after adding sodium chloride, and then micro-jet treatment; performing modification through circulation "preheating-micro-jet" combined treatment; and finally obtaining salt and heat double-resistant soybean protein powder through freeze drying. In the preheating stage, the method introduces sodium chloride, regulates the electrostatic interaction and hydration between protein molecules, reshapes the protein unfolding path, and promotes the cross-linking and recombination of the protein into controllable intermediate aggregates. Then, the protein is further broken and rearranged through the shearing, impact and cavity effect of high-pressure micro-jet, and the controllable aggregation and dynamic reconstruction are realized through multiple circulation, so that uniform and dense protein particles are finally formed. The soybean protein obtained by the method can still maintain good fluidity after being heated under high-protein concentration and high-salt conditions, and is suitable for salt-containing high-protein beverages and other related fields.

The present application relates to the technical field of food detection, in particular to a method for detecting the protein content in soy milk powder, comprising: obtaining the original near-infrared spectrum data of the soy milk powder sample to be detected and constructing a protein characteristic vector; calling a preset protein optical constant database storing the light absorption coefficient and refractive index under different protein concentrations, matching and then backstepping the average light path and extinction coefficient of the protein molecules to obtain light path extinction data; based on the data, establishing an absorption kinetics model to simulate the propagation process of light in the suspension and obtain the light intensity decay state, and after time integration, obtaining the cumulative absorbance proportional to the number of protein molecules; combining the dry weight of the sample to calculate the protein content per unit mass, and after environmental temperature compensation to correct the spectral drift, outputting the standardized detection result. The method can improve the accuracy and stability of the detection of the protein content in soy milk powder.

This invention discloses an anti-human MBP monoclonal antibody and its applications. The complementarity-determining region (CDR) of the heavy chain variable region of the monoclonal antibody has the amino acid sequences shown in SEQ ID NO. 1-3, and the CDR of the light chain variable region has the amino acid sequences shown in SEQ ID NO. 4-6. This invention uses a chemically synthesized polypeptide as an immunogen to immunize mice. Through cell fusion, screening, and subcloning, a monoclonal cell line that efficiently secretes anti-human MBP monoclonal antibodies is obtained. The antibody obtained by this invention has high specificity and high binding capacity, effectively targeting and binding MBP protein from serum. It is suitable for detecting MBP protein in blood, thereby evaluating neurological injury diseases that may cause changes in MBP protein concentration, and providing more convenient conditions for the diagnosis of mild traumatic brain injury.

The method for preparing water-soluble fertilizer by extracting protein from feather is a protein extraction technology, which comprises the following steps: first, pre-treatment of feather; second, drying; third, protein extraction, 100 parts of dried feather, 0.5-5% of catalyst fatty acid and 10-30% of water are fully mixed in a high-pressure container, and then the container is sealed; the temperature is increased to 160-220 degrees, the steam pressure is 0.5-2 MPa, and the process is continued for 5-20 minutes; when the temperature is reduced to 30-50 degrees, the container is taken out, at this time, the liquid protein concentration in the container is 45%-80%, and the amino acid concentration is 30%-70%; fourth, preparation of plant water-soluble fertilizer, the liquid protein with a protein concentration of 45% to 80% and 30-70% of amino acid liquid are subjected to enzymolysis by using protease, the amount of protease used is 1%-5%, the enzymolysis temperature is 30-50 degrees, the time is 24-72 hours, and then the temperature is increased to 100-120 degrees to inactivate the enzyme.

The utility model relates to the technical field of purification discloses a protein concentration purification two -effect evaporator for chicken powder preparation, including the evaporating bucket, the top end sliding connection of evaporating bucket has the fixed plate, the top end middle part of fixed plate is equipped with the accommodation groove, the top end rotation of fixed plate is connected with the fender, the inwall left side of accommodation groove is fixedly connected with control chip, the inwall right side of accommodation groove is fixedly connected with power regulator, the bottom middle part of fixed plate is fixedly connected with a plurality of inner layer heating pipe, the bottom of fixed plate is fixedly connected with a plurality of outer layer heating pipe, the inwall of evaporating bucket is fixedly connected with a plurality of temperature sensor. In the utility model, through control chip control servo motor, drive the stirring blade to the protein solution in the barrel stirring, simultaneously inner layer heating pipe and outer layer heating pipe cooperate heating, and servo motor two can drive auxiliary stirrer, reduce the temperature difference in the barrel, accelerate the evaporation speed of solution.

A UV-based imaging method for determining protein concentrations in unknown protein samples based on automated multi-wavelength calibration is disclosed. In various embodiments, a processor receives each of a set of standard wavelength data, such as data recorded by a detector, and a set of unknown wavelength data. Each of the set of standard wavelength data and the set of unknown wavelength data defines a series of absorbance-wavelength value pairs across a first wavelength range selected from a series of single-wavelength beams from the UV spectrum. The processor generates a multi-wavelength calibration model based on each of the first series of first absorbance-wavelength value pairs from the set of standard wavelength data. The processor implements the multi-wavelength calibration model to determine multiple protein concentration values ​​for each unknown protein sample in a given unknown protein sample.

This invention utilizes an insect-baculovirus expression system to prepare the recombinant protein SADS-CoV-S of porcine acute diarrhea syndrome coronavirus, which is then used to prepare egg yolk antibodies. By optimizing the composition of the subunit vaccine composition for immunizing laying hens, IL-2 and ZnCl2 solution are added as immunostimulants to the conventional adjuvant, increasing the antibody concentration in the egg yolk antibody extract and improving the neutralizing antibody titer and protective efficacy. When IL-2 and zinc chloride aqueous solution are added to the immunization composition simultaneously, the resulting egg yolk antibody aqueous solution exhibits the highest protein concentration, and at the same protein concentration, the egg yolk antibody has the highest neutralizing titer. Compared to the immunization composition using Freund's complete adjuvant alone, the neutralizing titer is increased by more than 60 times.

This invention belongs to the field of biomedical materials technology, specifically a mesenchymal stem cell exosome anti-fibrotic composition and its preparation and application in lung injury treatment. It uses human umbilical cord mesenchymal stem cell exosomes as the core active ingredient, combined with diammonium glycyrrhizate derivatives and N-acetylcysteine, and supplemented with pharmaceutical excipients. The human umbilical cord mesenchymal stem cell exosomes are obtained from healthy human umbilical cord tissue, purified through P3-P5 passage serum-free and xenogeneic protein-free culture, with a particle size distribution of 30-150 nm, a protein concentration of 200-800 μg / mL, and an exosome purity ≥95%. This invention is the first to propose a triple synergistic formula of "mesenchymal stem cell exosomes + diammonium glycyrrhizate derivatives + N-acetylcysteine." The exosomes exert a core tissue repair effect, the diammonium glycyrrhizate derivatives target and inhibit pulmonary inflammatory responses, and N-acetylcysteine ​​scavenge oxygen free radicals and resist oxidative stress. The synergistic effect of these three ingredients improves the anti-fibrotic and tissue repair effects by more than 40% compared to single exosome compositions, significantly enhancing repair efficiency.