3 results about "NGAL Protein" patented technology

The application belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody combination for detecting cat NGAL protein and application thereof. The monoclonal antibody combination of the application comprises monoclonal antibodies 1E2 and 2D6. The two antibodies have unique heavy chain and light chain variable region complementarity determining regions, and the amino acid sequences thereof have been defined in detail (SEQ ID NO. 1-12). The antibody combination can specifically recognize and efficiently bind to cat NGAL protein, and is suitable for developing a diagnostic tool with high sensitivity and high specificity. By constructing a double-antibody sandwich ELISA detection system, the minimum detection limit can reach 1 ng / mL, and good specificity and stability are shown.

This invention relates to the field of bioengineering technology, specifically to a method for preparing neutrophil gelatinase-associated lipid transport protein and its antibody. Specifically, using pET23a(+) as the expression vector, the NGAL gene with six His tags fused to its N-terminus under promoter control is introduced into *E. coli* for induced expression. After purification using a Ni Focurose 6FF column and a molecular sieve column, tag-free NGAL protein with a purity ≥95% is obtained. Subsequently, the NGAL protein is used to repeatedly immunize Japanese rabbits, and serum is collected after each immunization. Qualified serum is screened using a two-dimensional agar diffusion assay, and then purified using the aforementioned antigen to prepare an antigen affinity purification column, yielding a highly active NGAL polyclonal antibody. The high-purity recombinant NGAL of this invention not only possesses activity but is also low-cost, not limited by raw materials, and can be mass-produced, possessing potential and wide-ranging application value in clinical and in vitro diagnostics.

The application discloses an anti-NGAL recombinant antibody and a preparation and application thereof, and belongs to the technical field of antibodies. The anti-NGAL recombinant antibody is prepared by taking a recombinant NGAL protein antigen (21th-198th amino acid of human NGAL protein (P80188)) as an immunogen, and is obtained through B cell screening, heavy and light chain vector construction, expression and multiple rounds of screening and detection. The anti-NGAL recombinant antibody can specifically recognize NGAL protein and can be used for diagnosing diseases such as kidney diseases.