407 results about "Cell injury" patented technology

The invention discloses an extraction and purification method and application of plant-derived exosome-like nanoparticles, and relates to the technical field of plant exosome preparation. The extraction and purification method comprises the following steps: washing fresh ginseng, airing for later use, adding a proper amount of buffer solution, mixing, breaking walls, filtering, centrifuging filtrate to obtain supernate, carrying out ultracentrifugation on the supernate to obtain ginseng exosome nano-particle precipitates, resuspending the precipitates, respectively adding pure water sucrose solutions with different concentrations, and carrying out ultracentrifugation to obtain ginseng exosome nano-particles; and re-suspending the target strip, and carrying out ultracentrifugation to remove redundant cane sugar so as to obtain the purified ginseng exosome-like nano-particles. The ginseng exosome-like nano-particles disclosed by the invention have a remarkable effect when being applied to cosmetics or medicines with skin photoaging resistance, skin oxidation resistance and wrinkle resistance. In an anti-UVB (Ultraviolet B) induced cell injury experiment, the ginseng exosome-like nano-particles have an excellent anti-UVB induced cell injury effect.

The invention relates to the technical field of temperature regulation, in particular to an intelligent root temperature regulation system for hydroponic crops and an implementation method thereof, and the system comprises a root system oxygen consumption monitoring module, an osmotic pressure state monitoring module, a root system metabolism state analysis module, a root temperature parameter reconstruction module and a nutrient solution circulation linkage module. According to the method, the metabolic rhythm state of the root system is judged through oxygen consumption rate gradient difference calculation and trend analysis, the cell injury risk is recognized through two-parameter logic judgment by combining the osmotic pressure change amplitude and the oxygen consumption trend, and the target root temperature is selected through gray correlation analysis according to the abnormal triggering temperature and the stable state temperature; a regulation and control instruction is generated, a PID algorithm is called in combination with real-time circulation flow velocity data to adjust the pump speed, closed-loop dynamic matching of a root temperature set value and a flow velocity behavior is achieved, the adaptability of root temperature response is enhanced, the real-time regulation and control capacity for metabolic imbalance is improved, and the stability and the moisture and nutrient absorption efficiency of a water culture environment root system are effectively improved.

The invention discloses an RcWRKY29 gene and application of the RcWRKY29 gene in enhancing the resistance of Chinese roses to gray mold. The nucleotide sequence of the RcWRKY29 gene is shown as SEQ ID NO: 1. Researches find that the gene is specifically and highly expressed in Chinese rose petals, and the expression quantity is remarkably increased within 24-48 hours after botrytis cinerea infection. After RcWRKY29 expression is instantaneously inhibited through a virus-induced gene silencing technology, the scab area of Chinese rose petals is increased, and the disease resistance is remarkably reduced; otherwise, overexpression of the gene can significantly enhance disease resistance and reduce scab area and cell damage. The invention provides a recombinant vector containing RcWRKY29, an engineering bacterium and a detection kit, and develops a method for enhancing the disease resistance of Chinese rose based on an agrobacterium-mediated transformation technology. The invention provides a new target for Chinese rose gray mold resistance molecular breeding, can effectively reduce postharvest disease loss, and has important economic value and popularization and application prospects.

The present invention provides anticancer agents comprising as an active ingredient a bispecific antibody that comprises a domain comprising a glypican 3-binding antibody variable region, a domain comprising a T cell receptor complex-binding antibody variable region, and common L chains that can enhance the affinity for the two antigens, as well as pharmaceutical compositions comprising the bispecific antibody as an active ingredient, the compositions being for use in combination with other anticancer agents. The bispecific antibodies are novel molecules which are produced with high efficiency and have strong anti-tumor activity as well as safety and excellent pharmacodynamics. The bispecific antibodies can be expected to be applied to various cancers.

The invention relates to the technical field of biology, and particularly discloses a device and a method for separating an adherent cell through a vortex sound field, an ultrasonic module, piezoelectric ceramics and an Archimedes spiral structure concave lens are arranged to generate the vortex sound field to act on a cell culture dish, so that an adjustable shear flow is generated on the cell culture dish, and the adherent cell is separated from the cell culture dish. And locally separating the adherent cells in the cell culture dish. According to the concave lens with the Archimedes spiral structure, the problem of defocusing of a high-order vortex sound field can be solved, and the falling control accuracy of adherent cells is improved. The proposed method does not require any physical contact with adherent cells, and does not require insertion of an instrument into a cell culture dish, thereby reducing the risk of contamination and cell damage. The stable flow generated by absorbing sound energy by the liquid is used for indirectly separating the adherent cells, the method is extremely low in energy density and has good biocompatibility, and the activity of the cells is ensured while the cells are efficiently separated.

The invention discloses an intelligent cooperative regulation and control method and system for dissolved oxygen in a haematococcus culture solution, relates to the field of bioengineering, and controls a decision-making layer to dynamically switch between a growth promotion regulation and control mode and a stress induction regulation and control mode based on biomass data and physiological status data in a haematococcus culture stage so as to realize cooperative regulation and control of dissolved oxygen, illumination, pH and a carbon source. According to the method, a growth promotion mode and a stress induction mode are dynamically switched, biomass accumulation is promoted through dissolved oxygen sine wave pulse fluctuation in the vegetative period, astaxanthin synthesis is induced through high-oxygen and low-oxygen circulation and blue light regulation in the stress period, and compared with traditional static regulation, the biomass is increased, and the yield of astaxanthin is increased. A light-oxygen synergistic factor k is introduced to dynamically adjust the fluctuation range of dissolved oxygen, the problem of illumination-dissolved oxygen mismatching is solved, and the photosynthetic efficiency is improved; a stress intensity index SI is established, stress parameters are adaptively optimized based on the astaxanthin synthesis rate, cell damage caused by over-stress is avoided, and the stability of the astaxanthin synthesis rate in the stress period is improved.

The invention relates to the technical field of cell separation and culture, in particular to a method for separating and culturing haematochrome cells of plectropomus leopardus. The separation method of the plectropomus leopardus haematochrome cells is created for the first time, the EDTA digestive juice is used for pre-digesting epidermis cells on the outer layers of the plectropomus leopardus fins, then the collagenase digestive juice is used for digesting connective tissues (pigmented cells) of internal dermis, digestive enzymes such as trypsin and other mechanical methods which have great damage to the cells are not used, and the method is suitable for large-scale production of the plectropomus leopardus haematochrome cells. The plectropomus leopardus haematochrome cell can reduce cell damage, can be effectively separated to obtain the plectropomus leopardus haematochrome cell which is high in purity, good in activity and capable of being cultured in vitro, and lays a foundation for subsequent experiments such as single cell sequencing, cell in-vitro culture and molecular function verification.

The cell storage device comprises a storage box, the top of the storage box is provided with a material taking and placing port used for taking and placing test tubes into the storage box, the two ends of the interior of the storage box are symmetrically and fixedly connected with a first vertical frame and a second vertical frame, and lifting grooves are formed in the first vertical frame and the second vertical frame; lifting plates are slidably arranged in the lifting grooves, a supporting shaft is fixedly connected between the two lifting plates, cross-shaped plates are fixedly connected to the two ends of the supporting shaft, and cell storage mechanisms are rotatably connected between the corresponding ends of the two cross-shaped plates through bearings; the device has the beneficial effects that through the synergistic effect of the rotary cell storage mechanism and the liquid nitrogen freezing system, cell samples in all test tubes are uniformly exposed in a low-temperature environment, cell damage caused by non-uniform local temperature is avoided, and when cells are taken and placed, the test tube mounting rack can be controlled to be lifted to the position of a material taking and placing opening, so that the test tubes are conveniently taken and placed. The target sample can be accurately stored and taken without integrally taking out the storage rack.

The present invention belongs to the technical field of skin care, and discloses a skin repair composition and a skin care product, wherein the skin repair composition comprises the following components by weight percentage: 30-50% wheat germ oil, 1-5% ceramide 3, 1-10% sodium hyaluronate, and 40-60% bifid yeast fermentation product lysate. The present invention utilizes bifid yeast fermentation product lysate to help restore the activity of fibroblasts and keratinocytes, repair cell damage and protect the components in the extracellular matrix ECM, and further strengthens the damage repair speed of ECM by compounding sodium hyaluronate and ceramide, helps cells and extracellular matrix maintain a healthy state and quickly repair damage, and helps strengthen intercellular lipids and cell adhesion at the same time, and finally helps form a good skin surface sebum film through the synergistic effect of wheat germ oil and sodium hyaluronate, and the four cooperate with each other to significantly improve the skin repair effect.

The invention provides a cryopreservation and resuscitation method for induced differentiation of pluripotent stem cells to metaphase cells and application, and the cryopreservation method comprises the following steps: (1) the initial cells are pluripotent stem cells, and are induced and differentiated to the metaphase; (2) recovering the cells by using a mild digestive enzyme, counting, and resuspending the cells in a cryopreservation solution; and (3) carrying out programmed cooling to-80 DEG C, and then transferring into liquid nitrogen for preservation for later use. According to the cryopreservation method and the cryopreservation liquid provided by the invention, the cryopreservation survival rate of the differentiated metaphase cells is obviously improved, apoptosis or irreversible stress injury induced by a traditional cryopreservation liquid is avoided, and the problems of large cell injury and low survival rate caused by using a general cryopreservation liquid in the prior art are obviously improved. The resuscitated cells keep good differentiation potential, can smoothly form a mature renal unit structure, and solves the problem of subsequent differentiation failure in the prior art.

The invention relates to a fused exosome-like vesicle based on stem cell exosomes and application thereof, and the fused exosome-like vesicle is prepared by a method comprising the following steps: mixing stem cell exosomes and aloe exosome-like vesicles under an ultrasonic condition, and incubating to obtain the fused exosome-like vesicle. The fused exosome-like vesicle can effectively promote percutaneous penetration of stem cell exosome, and can effectively improve cell damage caused by ultraviolet rays, regenerate and repair skin damage barriers such as basilar membranes and the like, resist inflammation and oxidation, effectively remove melanin, and improve the skin quality. The composition has good repair potentials for chloasma and skin damage, aging and melanin deposition related to chloasma. Sufficient data support and technical support are provided for research and development of stem cell exosome innovative drugs, and clinical application of the stem cell exosome innovative drugs is accelerated.

Systemic lupus erythematosus (SLE) is an autoimmune disease in which circulating immune complexes can cause different types of glomerulonephritis, according to immune deposits and to the type of glomerular cell injury. Proliferative lesions represent the most severe form of lupus nephritis (LN) and often lead to kidney failure and death. Mucosal-associated invariant T (MAIT) are a subset of innate-like T cells that recognize microbial-derived ligands from the riboflavin synthesis pathway. Now the inventors analyzed the phenotype and function of peripheral blood MAIT cells by flow cytometry in 26 patients with LN and in a control group of 16 healthy volunteers. The inventors found that MAIT cell frequencies are markedly reduced in blood of LN patients. More particularly, among LN patients, baseline MAIT cell frequency, Ki-67 expression evaluating proliferative activity and granzyme B production measuring cytotoxicity represent promising prognostic factors of renal response one year after induction therapy. In conclusion, the inventors showed that baseline frequency and cytotoxic profile of MAIT cells may represent a promising prognostic factor of renal remission one year after induction therapy.

The invention belongs to the technical field of traditional Chinese medicine biology, and particularly relates to a phenolic diastereoisomer and a preparation method and application thereof.The phenolic diastereoisomer has the chemical structural formula shown in the formula I and the formula II, the formula I is dextroisomer and is named as (+)-fresh bamboo juice phenol D, and the formula II is levoisomer and is named as (-)-fresh bamboo juice phenol D. A pair of phenolic diastereoisomer compounds (+)-fresh bamboo juice phenol D and (-)-fresh bamboo juice phenol D are successfully obtained from fresh bamboo juice, and an anti-neurotoxicity activity test shows that (+)-fresh bamboo juice phenol D has a remarkable protection effect on PC12 cell injury caused by glutamic acid and can be used for preparing medicines for preventing or treating neurodegenerative diseases; wide application prospects are realized.

The invention discloses a radix gentianae callus cell culture and application method, and relates to the technical field of plant biology.The method comprises the following steps that in the laser irradiation process, a real-time spectrum analyzer is used for monitoring radix gentianae tissue, and absorption characteristic information of the plant tissue to light with different wavelengths is obtained. By combining the real-time spectral analysis and the machine learning model, the scheme can accurately monitor the interaction between the laser wavelength and the photosensitive component in the plant tissue, and by intelligently evaluating and dynamically adjusting the laser wavelength, the excessive absorption of laser energy is avoided, and the photochemical reaction and cell damage are reduced. The method not only reduces the potential damage of laser irradiation to plant cells, but also optimizes the synthesis process of medicinal components, and ensures the high efficiency, repeatability and reliability of callus culture. According to the scheme, powerful support is provided for improving the accuracy of experimental results and commercialized application of the gentiana scabra bunge.

The invention belongs to the technical field of organoid culture, and particularly relates to tissue digestive juice for organoid culture as well as a preparation method and application of the tissue digestive juice. According to the tissue digestive juice, a DMEM / F-12 culture medium which contains L-glutamine and does not contain 4-hydroxyethyl piperazine ethanesulfonic acid is used as a basic culture medium, calcium chloride, collagenase I type, collagenase III type, V type hyaluronidase, deoxyribonuclease I, 4-hydroxyethyl piperazine ethanesulfonic acid and Y-27632 are combined and have a synergistic effect, cell damage is reduced, meanwhile, the tissue digestive juice has the advantages that the tissue digestive juice can be used for improving the tissue digestive juice quality, and the tissue digestive juice has a good application prospect. The digestion time is shortened, the tissue digestion efficiency is improved, and the culture success rate of the organoid is improved.

The invention provides a composition for improving a barrier function and application thereof, and relates to the technical field of cell injury nursing. Gamma-aminobutyric acid (GABA) and cordyceps militaris fermentation filtrate (FCM) are used for treating barrier damage for the first time. Specifically, the combination of the gamma-aminobutyric acid and the cordyceps militaris fermentation filtrate can significantly improve damage of an epithelial mechanical barrier and an immune barrier caused by external stimulation, so that the epithelial barrier function can be prevented and improved from multiple dimensions.

The invention discloses application of SAA1 as a target spot in preparation of a medicine for preventing and treating kidney diseases, and belongs to the field of gene functions, application and biological medicine. In-vivo and in-vitro experiments prove that in an ischemia / reperfusion or ureteral obstruction induced mouse kidney disease model, the knockout of the SAA1 gene can relieve renal interstitial vascular endothelial cell injury, recover a renal capillary network, improve vascular permeability, reduce collagen deposition, inhibit renal fibrosis and effectively delay the progress of kidney diseases; furthermore, by adopting the SAA1 recombinant protein vaccine to immunize mice, the organism can be induced to generate a protective neutralizing antibody, and the SAA1 recombinant protein vaccine has a remarkable protective effect on kidney diseases induced by ischemia / reperfusion and ureteral obstruction. Therefore, the SAA1 is an intervention target for preventing and treating the kidney diseases, the SAA1 recombinant protein vaccine is an effective means for preventing and treating the kidney diseases, and active immunization by adopting the vaccine is an effective strategy for preventing and treating the kidney diseases.

The invention discloses an anti-aging composition for preventing light aging and application thereof, the anti-aging composition comprises a Rosa rugosa extract, a Caesalpinia spinosa fruit extract, a sunflower bud extract and a Pseudoalteromonas fermentation product extract, and the anti-aging composition can be added into various skin care products to reduce cell damage caused by light radiation such as ultraviolet light, infrared light and blue light; in sunscreen cosmetics, the sunscreen effect can be improved, the use amount of chemical sunscreen agents and physical sunscreen agents is reduced, and the safety and mildness of the sunscreen cosmetics are improved; damage caused by optical radiation is effectively prevented, meanwhile, skin elasticity is improved, skin wrinkles are reduced, and the effect of resisting skin photoaging is achieved. The effectiveness of the composition is proved through a large number of experiments, each component is irreplaceable, the addition range of the composition is also an optimal range, and the effect and cost of the composition are both the optimal range.

The invention discloses application of a neutralizing antibody of oncostatin M in preparation of a medicine for treating bronchial pulmonary dysplasia. The application proves that the intrauterine inflammation can cause BPD-like lung lesion to the filial generation mouse, and the OSM expression level in the mouse lung tissue one day after the intrauterine inflammation filial generation is grown is obviously higher than that of a control group; the OSM level of the BPD group is higher than that of the non-BPD group in the umbilical blood sample of the premature infant; in-vitro experiments show that the OSM treatment can cause lung epithelial cell injury; by dripping a recombined OSM neutralizing antibody into the nose of a newborn mouse, down-regulation of the OSM level in the lung of the mouse is realized, filial generation lung injury induced by the intrauterine inflammation can be repaired, and the OSM can be used as a treatment target of the BPD caused by the intrauterine inflammation.

The invention provides processed rhizoma polygonati polysaccharide, a preparation method thereof and application of the processed rhizoma polygonati polysaccharide in preparation of a medicine for treating renal injury. The nine-processed polygonatum sibiricum polysaccharide is named as NPCP and is composed of fructose and glucose, the NPCP is straight-chain inulin type polysaccharide with the polymerization degree of 12, and the structure of the NPCP is Glcp-(1-) and Fruf-(2-terminal,-1)-Fruf-(2-main chain. According to the obtained uniform nine-process polygonatum polysaccharide NPCP, the protective effect on adenine-induced HK-2 cell injury is achieved by improving the content of renal function markers, improving oxidative stress injury, reducing expression of proinflammatory cytokines, improving intestinal flora disorder and up-regulating short-chain fatty acid in the intestinal tract; more treatment directions are provided for effectively improving adenine-induced renal toxicity by the nine-process polygonatum polysaccharide, and theoretical and experimental bases are provided for clinically finding effective preparation of drugs for treating renal injury.

The invention relates to a double-response EGFR (epidermal growth factor receptor) targeted paclitaxel liposome based on heat shock protein, which adopts a pH sensitive design and is doped with heat shock protein 90 (HSP90) responsive molecule gambogic acid (GA), the surface of the liposome is modified with an EGFR antibody, and the core of the liposome is packaged with paclitaxel (PTX). According to the double-response EGFR targeted paclitaxel liposome of the heat shock protein, triple negative breast cancer cells are accurately locked through a double targeting strategy, and cell surface specific recognition is achieved through an EGFR antibody; gambogic acid GA responds to HSP90 overexpression in cells to promote drug release; the pH sensitive characteristic ensures that the medicine is quickly released in an acidic tumor microenvironment, the treatment effect is enhanced, and normal cell damage is reduced. According to the invention, the specific quick response release of the medicine is realized, the treatment accuracy and safety are improved, and the preparation method has a remarkable application prospect in the aspect of preparing the medicine for treating the triple-negative breast cancer.

The curcumin disclosed by the invention has the medicinal activity of preventing and treating mouse spermatogonial cell GC1 injury caused by triptolide, so that the curcumin has potential application value in the aspect of preparing medicines for preventing and treating spermatogonial cell injury caused by a tripterygium wilfordii preparation. Experimental results show that curcumin can be used for protecting spermatogonial cell damage caused by triptolide by regulating oxidative stress and reducing cell apoptosis. Therefore, a new thought and approach can be provided for clinical application of traditional Chinese medicine in male reproductive system injury repair. Furthermore, a new thought and a new approach are provided for clinical adjuvant medication of the triptolide, namely, the tripterygium wilfordii preparation containing the triptolide and the curcumin is developed, so that the anti-cancer drug activity and the like of the triptolide can be exerted, the reproductive injury side effect of the triptolide is inhibited or relieved through the curcumin, and the application prospect is wide. Spermatogonial cell injury induced by triptolide is effectively recovered, and particularly, spermatogonial cell oxidative stress injury and apoptosis caused by triptolide are effectively protected.

The invention belongs to the technical field of biology, and particularly relates to tilapia skin peptide, a preparation method thereof and application of the tilapia skin peptide in preparation of anti-aging related products. The tilapia skin peptide disclosed by the invention is IL, FGP or LPGL. In the invention, the tilapia skin is subjected to enzymolysis to prepare protein peptide, a peptide fragment with high content and high reliability is selected through mass spectrum identification, and a pure product is prepared by using a solid-phase chemical synthesis method. And by taking GPH as a positive control group, IL, FGP and LPGL are found to have a protection effect on UV-induced cell injury, have good antioxidant activity and an effect of repairing injured cells, and are beneficial to protecting a skin barrier and delaying skin aging.

The invention discloses a method for designing a bionic femoral stent based on fusion of a VT structure and a TPMS structure, and belongs to the technical field of bionic bone stents. The method comprises the following steps: respectively creating a Diamond unit homogeneous porous structure and a VT porous structure; and creating a Ramp fusion function, substituting the homogeneous porous structure of the Diamond unit and the Ramp fusion function into a Mix instruction, and then sequentially substituting the VT porous structures into the Mix instruction to generate a fusion model. The homogeneous porous structure of the Diamond unit and the VT porous structure are subjected to radial smooth fusion, smooth connection is realized at the fusion position, the service life of the porous structure serving as the bionic bone scaffold is shortened, the smooth surface of the D unit and the periodic structure uniformly conduct stress, the rounded Thiessen polygonal structure further disperses stress, and the stress dispersion and synergistic effects are achieved. The bionic femoral stent constructed by the invention can simulate natural bones, promote cell adhesion, proliferation and differentiation, and optimize transportation of nutrient substances and metabolic wastes. And the smooth fusion interface reduces cell damage and reduces immunological rejection.

The invention discloses a microfluidic image flow cytometry identification system and a microfluidic image flow cytometry identification method based on multi-modal phase imaging and deep learning, relates to a microfluidic image flow cytometry detection technology, and belongs to the crossing field of microfluidics, optical imaging and artificial intelligence. The cell deformation chip is used for inducing a cell sample to generate controllable deformation through the cell deformation chip and discharging deformed cells; the optical imaging unit is used for scanning the deformed cells and collecting multi-modal phase images of the deformed cells; the data processing and analysis unit is used for preprocessing the multi-modal phase image and performing cell intelligent identification analysis on the preprocessed multi-modal phase image by using a deep learning feature extraction network, so that accurate identification and classification of cells are realized, and a leukocyte subpopulation identification model is constructed; and generating a cell imaging result and a cell mechanical parameter thermodynamic diagram. According to the invention, label-free identification of leukocyte subgroups is realized, and the problems of tedious operation and cell damage of traditional fluorescence labeling are solved.

The invention discloses a neuroprotective peptide with anti-inflammatory and anti-oxidation effects and application of the neuroprotective peptide. The neuroprotective polypeptide is a polypeptide with any one of the following sequences: FPDFVYK and APDTRLF. The invention provides an antioxidant effect of the neuroprotective polypeptide in a PC12 cell injury model induced by hydrogen peroxide, and the neuroprotective polypeptide can be used for preparing antioxidant drugs. The invention provides an anti-inflammatory effect of the neuroprotective polypeptide in a PC12 cell injury model induced by hydrogen peroxide, and the neuroprotective polypeptide can be used for preparing anti-inflammatory drugs. The invention provides the influence of the neuroprotective polypeptide on the expression of pathways and neurotrophic factors in PC12 nerve cells. The neuroprotective polypeptide reduces the activity of acetylcholin esterase (AChE) and up-regulates the expression of neurotrophic factors so as to alleviate nerve cell damage and enhance memory, thereby achieving the purpose of preventing and treating neurodegenerative diseases.

The invention relates to the field of biological environmental protection, in particular to a heterotrophic nitrification immobilized engineering bacterium for efficient denitrification and a preparation method and application thereof.According to the heterotrophic nitrification immobilized engineering bacterium for efficient denitrification and the preparation method and application of the heterotrophic nitrification immobilized engineering bacterium, polyethylene glycol-glucan two-aqueous-phase concentration and trehalose protection liquid are adopted for synergistic effect, the bottleneck of cell damage caused by a traditional flocculation method can be avoided, trehalose molecules can wrap bacterial cells, and the bacterial cells can be effectively degraded; the immobilized particles can resist osmotic pressure impact and pollutant invasion, the survival rate of thalli is higher than 95%, so that the immobilized particles always keep ultrahigh denitrifying enzyme activity in various sewage environments, honeycomb-shaped pores are formed in an ethyl cellulose matrix, a microenvironment with a high specific surface area is provided for thalli attachment, and the built-in nano zeolite continuously and slowly releases Cu < 2 + > and Zn < 2 + > trace elements, so that the immobilized particles can be recycled. A thallus metabolism enzyme system is synergistically activated, and the denitrification pathway function is enhanced; the chitosan-polydopamine outer coating layer strongly captures the nitrogen-containing pollutants, so that the removal efficiency of the nitrogen-containing pollutants is improved; through triple protection, the density of unit granular bacteria is up to 109 cells / cm, the service life is prolonged, and the denitrification performance is improved by more than 40%.

The invention discloses a formula composition which is prepared from the following components in parts by weight: 100 to 400 parts by weight of grape seed procyanidine, 100 to 300 parts by weight of pomegranate polyphenol, 10 to 100 parts by weight of ergothioneine, 50 to 200 parts by weight of catechin and 100 to 400 parts by weight of astragalus polysaccharide. According to the invention, cell-level anti-aging is taken as a core logic, five active ingredients of grape seed procyanidine, pomegranate polyphenol, ergothioneine, catechin and astragalus polysaccharide are scientifically compounded, a multi-dimensional anti-oxidation defense system is constructed, and multiple effects of scavenging free radicals, repairing cell damage, delaying an aging process and the like can be realized.

The invention provides a biological preparation for regulating placenta dysplasia, and belongs to the technical field of gynaecology and obstetrics. Core effective components of the biological preparation are lutjanus mixed polypeptide with the molecular weight ranging from 2 kDa to 10 kDa, and the lutjanus mixed polypeptide is prepared by combining lutjanus fish bones, fish scales and fish flesh according to the mass ratio of 5: 3: 2 through the steps of desalting treatment, ultrasonic-assisted degreasing, double-enzyme synergistic enzymolysis, double ultrafiltration purification and the like. Experimental results show that the lutjanus mixed polypeptide has a remarkable protection effect on a human trophoblast cell line HTR-8 / SVneo in a high-glucose environment, and can effectively promote cell proliferation and migration and reduce high-glucose-induced cell damage. Therefore, the biological preparation provides a novel effective intervention means for placenta dysplasia caused by gestational diabetes mellitus, and has a good application prospect.

The invention relates to a novel spirobenzylisoquinoline alkaloid hypecoum erectum ketone base C extracted from hypecoum erectum as well as a preparation method and application of the novel spirobenzylisoquinoline alkaloid hypecoum erectum ketone base C, which can be used for effectively extracting and preparing the novel spirobenzylisoquinoline alkaloid hypecoum erectum ketone base C from the hypecoum erectum and realizing the application in preparing a medicine for preventing and treating a non-alcoholic fatty liver disease. Carrying out heating reflux extraction on the dried hypecoum erectum by using ethanol, carrying out acid extraction and alkali precipitation, and sequentially extracting by using chloroform and n-butyl alcohol to obtain an extract of a corresponding part; and mixing the target extract with silica gel, eluting, concentrating under reduced pressure, detecting, combining the same fractions, eluting on a column, mixing the silica gel with the sample, eluting, collecting the fractions with retention time, concentrating and drying to obtain the anise keto alkali C. The invention also discloses a preparation method of the anise keto alkali C. The invention also discloses a preparation method of the anise keto alkali C. The compound can significantly improve free fatty acid-induced HepG2 cell lipid metabolism disorder and lipid accumulation, meanwhile alleviate FFA-induced HepG2 cell damage, can be prepared into various drug dosage forms, is convenient and effective in clinical use, has wide development and application prospects, and has significant economic and social benefits.