187 results about "Cell injury" patented technology

The invention relates to the technical field of biology, and particularly discloses a device and a method for separating an adherent cell through a vortex sound field, an ultrasonic module, piezoelectric ceramics and an Archimedes spiral structure concave lens are arranged to generate the vortex sound field to act on a cell culture dish, so that an adjustable shear flow is generated on the cell culture dish, and the adherent cell is separated from the cell culture dish. And locally separating the adherent cells in the cell culture dish. According to the concave lens with the Archimedes spiral structure, the problem of defocusing of a high-order vortex sound field can be solved, and the falling control accuracy of adherent cells is improved. The proposed method does not require any physical contact with adherent cells, and does not require insertion of an instrument into a cell culture dish, thereby reducing the risk of contamination and cell damage. The stable flow generated by absorbing sound energy by the liquid is used for indirectly separating the adherent cells, the method is extremely low in energy density and has good biocompatibility, and the activity of the cells is ensured while the cells are efficiently separated.

The invention provides a cryopreservation and resuscitation method for induced differentiation of pluripotent stem cells to metaphase cells and application, and the cryopreservation method comprises the following steps: (1) the initial cells are pluripotent stem cells, and are induced and differentiated to the metaphase; (2) recovering the cells by using a mild digestive enzyme, counting, and resuspending the cells in a cryopreservation solution; and (3) carrying out programmed cooling to-80 DEG C, and then transferring into liquid nitrogen for preservation for later use. According to the cryopreservation method and the cryopreservation liquid provided by the invention, the cryopreservation survival rate of the differentiated metaphase cells is obviously improved, apoptosis or irreversible stress injury induced by a traditional cryopreservation liquid is avoided, and the problems of large cell injury and low survival rate caused by using a general cryopreservation liquid in the prior art are obviously improved. The resuscitated cells keep good differentiation potential, can smoothly form a mature renal unit structure, and solves the problem of subsequent differentiation failure in the prior art.

The invention provides a composition for improving a barrier function and application thereof, and relates to the technical field of cell injury nursing. Gamma-aminobutyric acid (GABA) and cordyceps militaris fermentation filtrate (FCM) are used for treating barrier damage for the first time. Specifically, the combination of the gamma-aminobutyric acid and the cordyceps militaris fermentation filtrate can significantly improve damage of an epithelial mechanical barrier and an immune barrier caused by external stimulation, so that the epithelial barrier function can be prevented and improved from multiple dimensions.

The invention discloses a microfluidic image flow cytometry identification system and a microfluidic image flow cytometry identification method based on multi-modal phase imaging and deep learning, relates to a microfluidic image flow cytometry detection technology, and belongs to the crossing field of microfluidics, optical imaging and artificial intelligence. The cell deformation chip is used for inducing a cell sample to generate controllable deformation through the cell deformation chip and discharging deformed cells; the optical imaging unit is used for scanning the deformed cells and collecting multi-modal phase images of the deformed cells; the data processing and analysis unit is used for preprocessing the multi-modal phase image and performing cell intelligent identification analysis on the preprocessed multi-modal phase image by using a deep learning feature extraction network, so that accurate identification and classification of cells are realized, and a leukocyte subpopulation identification model is constructed; and generating a cell imaging result and a cell mechanical parameter thermodynamic diagram. According to the invention, label-free identification of leukocyte subgroups is realized, and the problems of tedious operation and cell damage of traditional fluorescence labeling are solved.

The invention discloses a neuroprotective peptide with anti-inflammatory and anti-oxidation effects and application of the neuroprotective peptide. The neuroprotective polypeptide is a polypeptide with any one of the following sequences: FPDFVYK and APDTRLF. The invention provides an antioxidant effect of the neuroprotective polypeptide in a PC12 cell injury model induced by hydrogen peroxide, and the neuroprotective polypeptide can be used for preparing antioxidant drugs. The invention provides an anti-inflammatory effect of the neuroprotective polypeptide in a PC12 cell injury model induced by hydrogen peroxide, and the neuroprotective polypeptide can be used for preparing anti-inflammatory drugs. The invention provides the influence of the neuroprotective polypeptide on the expression of pathways and neurotrophic factors in PC12 nerve cells. The neuroprotective polypeptide reduces the activity of acetylcholin esterase (AChE) and up-regulates the expression of neurotrophic factors so as to alleviate nerve cell damage and enhance memory, thereby achieving the purpose of preventing and treating neurodegenerative diseases.

The invention relates to the field of biological environmental protection, in particular to a heterotrophic nitrification immobilized engineering bacterium for efficient denitrification and a preparation method and application thereof.According to the heterotrophic nitrification immobilized engineering bacterium for efficient denitrification and the preparation method and application of the heterotrophic nitrification immobilized engineering bacterium, polyethylene glycol-glucan two-aqueous-phase concentration and trehalose protection liquid are adopted for synergistic effect, the bottleneck of cell damage caused by a traditional flocculation method can be avoided, trehalose molecules can wrap bacterial cells, and the bacterial cells can be effectively degraded; the immobilized particles can resist osmotic pressure impact and pollutant invasion, the survival rate of thalli is higher than 95%, so that the immobilized particles always keep ultrahigh denitrifying enzyme activity in various sewage environments, honeycomb-shaped pores are formed in an ethyl cellulose matrix, a microenvironment with a high specific surface area is provided for thalli attachment, and the built-in nano zeolite continuously and slowly releases Cu < 2 + > and Zn < 2 + > trace elements, so that the immobilized particles can be recycled. A thallus metabolism enzyme system is synergistically activated, and the denitrification pathway function is enhanced; the chitosan-polydopamine outer coating layer strongly captures the nitrogen-containing pollutants, so that the removal efficiency of the nitrogen-containing pollutants is improved; through triple protection, the density of unit granular bacteria is up to 109 cells / cm, the service life is prolonged, and the denitrification performance is improved by more than 40%.

The invention belongs to the field of biological medicines, and particularly relates to application of a medicine for intervening in a mitochondrial pyruvic acid carrier in preparation of a medicine for treating acute pancreatitis and a medicine composition of the medicine. The medicine for intervening in the mitochondrial pyruvic acid carrier can reduce the serum enzyme level, relieve pancreatic pathological injury, inhibit inflammatory response, inhibit calcium overload and protect the mitochondrial function, so that pancreatic acinus cell injury is relieved, the effect of treating acute pancreatitis is achieved, and the medicine has a wide application prospect. The invention provides a new choice for clinically treating acute pancreatitis, and has a good application prospect.

The application discloses a kind of acanthopanax acid polysaccharides with intestinal protection function and its preparation method and application, belong to the field of functional food and biotechnology.The acanthopanax acid polysaccharide is by water extraction alcohol precipitation, after impurity removal, through DEAE-52 anion exchange column elution and collection 0.2 mol / L NaCl elution peak, and the uniform component obtained by Sephadex G-100 gel column chromatography purification.Its uronic acid content is 30%-40%, weight average relative molecular weight is 50-100kDa, mainly by glucose, galactose, galacturonic acid and arabinose, infrared spectrum has carboxyl characteristic absorption peak at 1736 cm-1 and 1615 cm-1.Experiments prove that the acanthopanax acid polysaccharide has antioxidant activity and hypoglycemic activity.The acid polysaccharide has protective effect on LPS-induced Caco-2 cell damage, can effectively reduce the secretion of pro-inflammatory factors TNF-α, IL-1β, IL-6, and improve the level of anti-inflammatory factor IL-10.The acanthopanax acid polysaccharide can be prepared into oral liquid, tablet candy, solid beverage and other functional food forms.

The application discloses application of skin precursor cell induced Schwann cell derived vesicles (SKP-SC-EVs) in preparation of a product for treating Parkinson's disease, wherein the SKP-SC-EVs have a protective effect in rotenone or MPP+-induced cell injury, and in a MPTP-induced mouse Parkinson's disease model, the SKP-SC-EVs can be given by a nasal instillation method to improve the motor ability of the mouse and restore the olfactory function, and the product can be used for research and treatment of Parkinson's disease.

This invention relates to the fields of biomedical technology and biological model construction technology, and discloses an ANCA-associated vasculitis glomerular endothelial cell injury model, its construction method and application, and the application of BACH1 inhibitors. The method includes: (1) activating neutrophils sequentially with tumor necrosis factor α and immunoglobulin G to obtain a culture medium containing activated neutrophils; wherein the immunoglobulin G is immunoglobulin G isolated and purified from the plasma of patients with ANCA-associated vasculitis; (2) co-culturing the culture medium containing activated neutrophils with human glomerular endothelial cells to obtain the cell injury model. The cell injury model provided by this invention can verify the role of neutrophil adhesion characteristics in endothelial cell injury, and at the same time, the cell injury model can better simulate the immune microenvironment of ANCA-associated vasculitis glomerular endothelial cells in vivo.

A method for increasing or improving nitric oxide signals or levels, the method comprising: administering a composition to a subject, the composition comprising: arginine or a pharmaceutically acceptable salt, ester, or derivative thereof; and tetrahydrocurcumin and / or β-aminoisobutyric acid or a pharmaceutically acceptable salt or ester thereof. The method and the composition can be used to increase, improve, or enhance nitric oxide signals or levels, inhibit nitric oxide decomposition, and increase or enhance nitrous acid and / or cyclic guanosine monophosphate levels, and can improve fatigue resistance or enhance muscle strength, maintain or improve cardiovascular health, maintain or improve vascular function and structure, inhibit or mitigate endothelial cell damage, or mitigate insufficient blood flow.

The invention provides a cell activity detection method based on fluorescence labeling, and relates to the technical field of cell analysis. The cell activity detection method based on fluorescence labeling comprises the following steps: S1, co-incubating a cell sample to be detected with a mixed solution of propidium iodide, a JC-1 mitochondrial membrane potential dye, a DCFH-DA active oxygen probe, a Caspase-3 / 7 activated substrate and a Hoechst nuclear dye, completing synchronous multiple labeling, and generating a multi-labeled cell sample, S2, detecting the cell activity based on the multi-labeled cell sample, five-channel full-automatic scanning is carried out through a high-content imaging system, Z-stack images are collected, and a multispectral image data set is generated. The five probes are mixed and incubated at one time through a synchronous multiple labeling process, so that the operation time is remarkably shortened, and the risk of cell injury caused by multiple times of centrifugation is reduced; z-stack full-automatic scanning is combined with three-dimensional image acquisition, the capturing capability of weak fluorescence signals is improved through multi-focal plane fusion, and information loss caused by cell thickness or position offset in traditional two-dimensional imaging is overcome.

The application provides a freezing and recovery method and application of pluripotent stem cell induced differentiation middle stage cells, and the freezing method comprises the following steps: (1) the starting cells are pluripotent stem cells, which are induced to the middle stage; (2) the cells are recovered and counted by using a mild digestion enzyme, and the cells are resuspended in a freezing solution; (3) after programmed cooling to-80 DEG C, the cells are transferred into liquid nitrogen for storage, and are ready for use. The freezing method and the freezing solution provided by the application significantly improve the freezing survival rate of the differentiation middle stage cells, avoid the apoptosis or irreversible stress damage induced by the traditional freezing solution, and obviously improve the problems of cell damage and low survival rate caused by the general freezing solution in the prior art. The differentiation potential of the recovered cells is good, and the cells can successfully form mature kidney unit structures, so that the problem of subsequent differentiation failure in the prior art is solved.

The application discloses a design method and device of a force-position switching controller of a piezoelectric-driven cell microinjector and an injection method thereof, and comprises the following steps: S1: based on the deformation and viscoelasticity of cells, a mass-spring-damper system is used to establish a dynamic model for describing a system formed by the microinjector and the cells; S2: based on the dynamic model, an adaptive integral terminal sliding mode impedance force controller is designed; S3: based on a feedforward compensator and a feedback control law, a composite PID position controller is designed; S4: a switching function is designed; and S5: the switching control law of the final force-position switching controller is calculated by combining the adaptive integral terminal sliding mode impedance force controller, the PID position controller and the switching function. The force-position switching controller can provide accurate force and position tracking performance in each control mode, and still has small chattering and cell damage degree when different modes are switched multiple times.

The invention discloses an organoid culture medium, a kit and a method for culturing an organoid, and relates to the technical field of organoid culture. The organoid culture medium provided by the invention can improve the proliferation activity of the organoid, a relatively complete organoid form can be obtained through culture, and the cost of the culture medium is reduced. The tumor cell organoid obtained by adopting the organoid culture medium provided by the invention has a more complete tumor cell surface marker, and the cell damage is smaller.

The method comprises the following steps: taking red ginseng, radix ophiopogonis and schisandra chinensis, carrying out water extraction and freeze drying to obtain freeze-dried powder, taking the freeze-dried powder, adding distilled water to dissolve the freeze-dried powder, adding the dissolved freeze-dried powder to a macroporous adsorption resin column, carrying out gradient elution with water, a 20% ethanol aqueous solution, a 50% ethanol aqueous solution and a 75% ethanol aqueous solution in sequence, and carrying out vacuum concentration to obtain the effective part of the pulse-activating decoction. From the beginning of sample loading, collecting every 300mL of effluent as a fraction, and collecting the fractions Fr1-Fr16; the chromatographic characteristics are analyzed, fractions with the same chromatographic characteristics are combined, the fractions Fr15-Fr16 are combined into a component yf-10, and the component yf-10 is the effective part of the pulse-activating decoction. According to the effective part disclosed by the invention, toxic parts are removed, so that H9c2 cell injury caused by adriamycin can be protected in a more effective, more stable and lower-toxicity manner, and the application of the component yf-10 in preparation of medicines for treating myocardial cell injury is prompted.

The invention provides a traditional Chinese medicine preparation for improving or treating cognitive impairment, and relates to the technical field of traditional Chinese medicine pharmacy, and the traditional Chinese medicine preparation is prepared from morinda officinalis, eclipta, semen boitae, fructus alpiniae oxyphyllae, bighead atractylodes rhizome, valerian, turmeric, rhizoma acori graminei, leech, rhizoma anemarrhenae, dendrobium officinale, endothelium corneum gigeriae galli, hematoxylon and honey-fried licorice root. The traditional Chinese medicine preparation shows remarkable neuroprotection and cognition improvement effects on the cell level and the animal level, in the cell level, the preparation can effectively resist Abeta1-42 induced cell damage, and the protection effect is achieved through multiple ways of improving the cell activity, enhancing the oxidation resistance, inhibiting neuroinflammation and the like; in animal experiments, the preparation can comprehensively improve cognitive impairment of APP / PS1 model mice, including relieving behavioral abnormality, improving spatial learning and memory ability, enhancing new object recognition ability, and effectively reducing deposition of A beta in hippocampus on a molecular level. The invention provides a new thought for improving or treating cognitive impairment.

The invention relates to the technical field of noise deafness susceptibility grading, in particular to a noise deafness susceptibility grading method and system based on a multi-band hearing threshold, and the method comprises the steps: presetting measurement frequency points, generating a measurement frequency point set according to a sequence from high frequency to low frequency, and completing M times of independent measurement for each frequency point; recording a tested subjective response timestamp, and generating a confidence value label; an otoacoustic emission signal in a tested ear canal is collected in real time, a time domain signal with the highest confidence coefficient of a single frequency point is extracted, spectrum features are calculated, and otoacoustic emission joint parameters are constructed. According to the method, the time sequence diffusion rule from high frequency to low and medium frequency hair cell damage can be comprehensively captured, the identification accuracy of potential auditory impairment is remarkably improved, the propagation mechanism from high frequency damage to intermediate frequency can be simulated by constructing the physically constrained spectrum migration operator, and otoacoustic emission time domain-frequency domain joint parameters and physiological indexes are combined, so that the identification accuracy of the potential auditory impairment is improved. And individualized and dynamic injury risk prediction is realized.

The invention discloses a design method and device of a force-position switching controller of a piezoelectrically-driven cell microinjector and an injection method of the piezoelectrically-driven cell microinjector, and the design method comprises the following steps: S1, based on deformation and viscoelasticity of cells, establishing a dynamic model for describing a system formed by the microinjector and the cells by adopting a mass-spring-damper system; s2, designing a self-adaptive integral terminal sliding mode impedance force controller based on the dynamic model; s3, designing a composite PID position controller based on the feedforward compensator and the feedback control law; s4, designing a switching function; and S5, in combination with the adaptive integral terminal sliding mode impedance force controller, the PID position controller and the switching function, calculating to obtain a final switching control rule of the force-position switching controller. The force-position switching controller provided by the invention can provide accurate force and position tracking performance in each control mode, and still has small buffeting and cell damage degree when different modes are switched for multiple times.

The invention discloses a targeting peptide for blocking combination of IL-17A and IL-17RA / RC and application thereof, the targeting peptide is selected from AZS-1, AZS-2, AZS-3, AZS-4 and AZS-5 or a combination of any one of AZS-1, AZS-2, AZS-3, AZS-4 and AZS-5 and IBP, the amino acid sequence of AZS-1 is as shown in SEQ ID NO: 1, the amino acid sequence of AZS-2 is as shown in SEQ ID NO: 2, the amino acid sequence of AZS-3 is as shown in SEQ ID NO: 3, the amino acid sequence of AZS-4 is as shown in SEQ ID NO: 4, and the amino acid sequence of AZS-5 is as shown in SEQ ID NO: 5. The amino acid sequence of the AZS-5 is as shown in SEQ ID NO: 5, and the amino acid sequence of the IBP is as shown in SEQ ID NO: 6. The targeting peptide provided by the invention has good tolerance, can generate effective interaction with the surface of a target protein, can realize specific binding with IL-17A, has relatively high blocking ability, can effectively improve symptoms of liver and kidney cell injury, cell edema and skin injury caused by psoriasis after being applied to a psoriasis mouse model, and has a good application prospect. The compound has a good application prospect in treatment and / or adjuvant therapy of IL-17A-mediated inflammatory diseases.

Described herein are pumps that linearly reciprocate to assist with circulating blood within the body of a patient. Red blood cell damage may be avoided or minimized by such linear pump movement. The linearly reciprocating movement may also generate a pulsatile pumping cycle that mimics the natural pumping cycle of the heart. The pumps may be configured to reside at various body locations. For example, the pumps may be situated within the right ventricle, the left ventricle, the ascending aorta, the descending aorta, the thoracic aorta, or the abdominal aorta. In some instances, the pump may be deployed within the venous circulation. In other instances, the pump may reside outside the patient.

The invention discloses a pharmaceutical composition and application of reniminase in preparation of a medicine for treating microvascular occlusion, and belongs to the technical field of biological medicines. The invention provides an application of reniminase in preparation of a medicine for treating microvascular occlusion. In addition, the invention further provides a pharmaceutical composition for treating microvascular occlusion, the pharmaceutical composition comprises a vector for overexpressing reniminase, and the vector is an adeno-associated virus vector or a plasmid vector. The reniminase provided by the invention reduces MVO by improving the mitochondrial function of endothelial cells and reducing oxidative stress and cell damage. The mechanism provides a new thought for treatment of cardiovascular diseases, especially MVO in STEMI patients.

The invention belongs to the technical field of biological medicine, and relates to a novel application of endogenous substances in alcohol related liver disease (ALD). The invention proposes for the first time that an endogenous substance with regular concentration change in the ALD process can be used as a biomarker for auxiliary diagnosis and / or stage evaluation of ALD, the exogenous supplement of the endogenous substance has a prevention and treatment effect on ALD, and the endogenous substance has double values of being used as an excellent biomarker and an effective therapeutic agent. The substances include but are not limited to taurochenodeoxycholic acid (TCDCA), dehydroepiandrosterone sulfate (DHEA-S) and the like. Wherein the serum concentration of TCDCA is obviously increased along with disease progression, and the concentration of DHEA-S is obviously reduced. The diagnostic value of the substance on ALD is remarkably superior to that of a traditional liver injury marker, and ethanol-induced liver cell injury and mouse liver pathological change can be remarkably improved through exogenous supplementation. The invention provides a brand new strategy for accurate diagnosis and prevention of ALD.

The application relates to the technical field of medicines, and particularly discloses a polypeptide and application of the polypeptide in preparation of a medicine for ischemic stroke. The amino acid sequence of the polypeptide is as shown in SEQ ID NO. 1. The polypeptide can significantly improve the nerve function defect of a mouse transient middle cerebral artery occlusion / reperfusion (tMCAO / R) model in vivo, reduce the cerebral infarction volume, and reduce the whole-body oxidative stress level. In vitro, the polypeptide has a direct protective effect on HT22 neuron cells and hCMEC / D3 brain microvascular endothelial cells injured by oxygen-glucose deprivation (OGD) induction, and can resist the injury of PC12 cells induced by corticosterone (CORT) and MPP + , and effectively inhibit the accumulation of reactive oxygen species (ROS) in cells. The results show that the polypeptide has a multi-target cell protection activity, and can play an anti-ischemic stroke role from multiple dimensions such as reduction of infarction volume, inhibition of oxidative stress level, and protection of nerve and vascular units.

The invention discloses an intracellular biomass detection method with small damage to cells and low disturbance, and relates to the technical field of cell detection. The invention provides the intracellular biomass detection method with small damage to cells and low disturbance, which depends on the integrated intracellular biomass detection device and adopts an electroporation sampling mode to carry out molecular sampling, so that the activity damage to the cells is avoided; electroporation sampling and nanopore detection are integrated, a one-way main flow channel of a Tesla valve structure is introduced in a matched mode so as to achieve physical communication and eliminate reverse flow on the fluid side, transfer is completed through a one-way path, the high cell viability is kept, and extracted biomolecules do not need to be manually transferred.

This invention discloses a method for assessing the toxicity of antibiotics to green algae based on Fourier transform infrared spectroscopy, comprising: constructing a multidimensional experimental dataset containing different antibiotic concentrations and exposure times under controlled conditions, and simultaneously acquiring raw infrared spectra and degradation efficiency data of green algae. The spectral data are preprocessed to extract toxic response features characterizing cell damage and metabolic feedback features reflecting metabolic activity, forming a dual-path spectral feature set. A heterogeneous information network containing toxic effect edges and degradation feedback edges is constructed using antibiotic concentration and green algae state as nodes. A toxicity joint inference model is constructed based on a graph neural network, and the model is trained using the heterogeneous information network to achieve synergistic analysis of toxic effects and degradation potential. The trained model is used to analyze the target water area situation, simulate the purification process, and generate a dynamic river purification planning report including nodes for biomass maintenance and auxiliary intervention measures, providing comprehensive and accurate purification decision support.

The disclosure provide compounds of Formula I and the pharmaceutically acceptable salts thereof. The variables, R1, R2, R3, X1, X2, and Z are defined herein. Certain compounds of Formula I act as selective mitochondrial protonophore uncouplers that do not affect the plasma membrane potential. Compounds and salts of Formula I are useful for treating or decreasing the risk of conditions responsive to mitochondrial uncoupling, such as cancer, obesity, type II diabetes, fatty liver disease, insulin resistance, Parkinson's disease, ischemia reperfusion injury, heart failure, non-alcoholic fatty liver disease (NALFD), and non-alcoholic steatohepatitis (NASH). Because mitochondrial uncouplers decrease the production of reactive oxygen species (ROS), which are known to contribute to age-related cell damage, compounds of Formula I are useful for increasing lifespan. Compounds and salts of Formula I are also useful for regulating glucose homeostasis or insulin action in a patient.

The invention relates to plant source dry-state mitochondria for cell repair and aging resistance as well as a preparation method and application of the plant source dry-state mitochondria. The plant source dry-state mitochondria can effectively improve functions of mitochondria, reduce the damage degree of cells, inhibit cell aging and effectively repair skin wounds. According to the method, the mitochondria is extracted from healthy plant tissues, so that the production cost is reduced, and a harsh quarantine step is not needed. According to the present invention, the dry-state processing enables the mitochondria storage condition to be loose, the transportation and storage difficulty and the transportation and storage cost can be further reduced, the plant source mitochondria subjected to the dry-state processing has characteristics of flexible dosage form, wide auxiliary material application range, and meeting of the design requirements of various products, and the dry-state processing can further remove the nucleic acid in the extract and eliminate the inflammation of the product; in the research process, the inventor also finds that after the plant source mitochondria is subjected to dry-state processing, the mitochondria function can be more effectively improved, the cell damage degree is reduced, cell aging is inhibited, and the skin wound surface is effectively repaired.

This invention discloses a sulfated arabinoglycoside, its preparation method, and its applications, belonging to the field of biomedical technology. The technical solution includes a sulfated arabinoglycoside derived from *Streptococcus linearis* polysaccharide; a degree of polymerization of 2-5; arabinose as the main monosaccharide, and containing galactose; the molar percentage of arabinose is not less than 80%, and the molar percentage of galactose is not more than 20%; the main chain is composed of (1→4)-β-L-pyranose arabinose groups, with the sulfate groups of the arabinose mainly substituted at the C-3 position. This invention is applied to inhibiting the abnormal aggregation of human pancreatic islet amyloid peptide (hIAPP), solving the problem of the lack of safe and effective intervention methods for the abnormal aggregation of human pancreatic islet amyloid peptide in existing technologies. It features stable raw material sources, high safety, a simple and mild preparation method suitable for large-scale preparation, and the ability to inhibit the abnormal aggregation of human pancreatic islet amyloid peptide and reduce pancreatic β-cell damage.

The invention relates to the technical field of daily cosmetics, and particularly provides a preparation method and application of a katsumadai katsumadai extract. In the preparation process of the alpinia katsumadai extract, an ethanol / water mixed solvent (preferably 75 + / -5% (v / v)) with a specific concentration is used as an extraction solvent, the solvent is safe and non-toxic, and the alpinia katsumadai extract obtained by enrichment after purification through macroporous resin (preferably ADS-17) has excellent dual UV absorption performance of absorbing UVA and absorbing UVB; the alpinia katsumadai extract disclosed by the invention is a plant component, has the advantages of high safety, low irritation, no cell damage, no influence on cell viability, extremely small or no side effect, wide applicable crowd, and is very suitable for being used as an active component of cosmetics, and is particularly suitable for being used for preparing sunscreen cosmetics.