324 results about "Cell injury" patented technology

The invention discloses an extraction and purification method and application of plant-derived exosome-like nanoparticles, and relates to the technical field of plant exosome preparation. The extraction and purification method comprises the following steps: washing fresh ginseng, airing for later use, adding a proper amount of buffer solution, mixing, breaking walls, filtering, centrifuging filtrate to obtain supernate, carrying out ultracentrifugation on the supernate to obtain ginseng exosome nano-particle precipitates, resuspending the precipitates, respectively adding pure water sucrose solutions with different concentrations, and carrying out ultracentrifugation to obtain ginseng exosome nano-particles; and re-suspending the target strip, and carrying out ultracentrifugation to remove redundant cane sugar so as to obtain the purified ginseng exosome-like nano-particles. The ginseng exosome-like nano-particles disclosed by the invention have a remarkable effect when being applied to cosmetics or medicines with skin photoaging resistance, skin oxidation resistance and wrinkle resistance. In an anti-UVB (Ultraviolet B) induced cell injury experiment, the ginseng exosome-like nano-particles have an excellent anti-UVB induced cell injury effect.

The invention relates to the technical field of temperature regulation, in particular to an intelligent root temperature regulation system for hydroponic crops and an implementation method thereof, and the system comprises a root system oxygen consumption monitoring module, an osmotic pressure state monitoring module, a root system metabolism state analysis module, a root temperature parameter reconstruction module and a nutrient solution circulation linkage module. According to the method, the metabolic rhythm state of the root system is judged through oxygen consumption rate gradient difference calculation and trend analysis, the cell injury risk is recognized through two-parameter logic judgment by combining the osmotic pressure change amplitude and the oxygen consumption trend, and the target root temperature is selected through gray correlation analysis according to the abnormal triggering temperature and the stable state temperature; a regulation and control instruction is generated, a PID algorithm is called in combination with real-time circulation flow velocity data to adjust the pump speed, closed-loop dynamic matching of a root temperature set value and a flow velocity behavior is achieved, the adaptability of root temperature response is enhanced, the real-time regulation and control capacity for metabolic imbalance is improved, and the stability and the moisture and nutrient absorption efficiency of a water culture environment root system are effectively improved.

The invention discloses an RcWRKY29 gene and application of the RcWRKY29 gene in enhancing the resistance of Chinese roses to gray mold. The nucleotide sequence of the RcWRKY29 gene is shown as SEQ ID NO: 1. Researches find that the gene is specifically and highly expressed in Chinese rose petals, and the expression quantity is remarkably increased within 24-48 hours after botrytis cinerea infection. After RcWRKY29 expression is instantaneously inhibited through a virus-induced gene silencing technology, the scab area of Chinese rose petals is increased, and the disease resistance is remarkably reduced; otherwise, overexpression of the gene can significantly enhance disease resistance and reduce scab area and cell damage. The invention provides a recombinant vector containing RcWRKY29, an engineering bacterium and a detection kit, and develops a method for enhancing the disease resistance of Chinese rose based on an agrobacterium-mediated transformation technology. The invention provides a new target for Chinese rose gray mold resistance molecular breeding, can effectively reduce postharvest disease loss, and has important economic value and popularization and application prospects.

The invention relates to the technical field of biology, and particularly discloses a device and a method for separating an adherent cell through a vortex sound field, an ultrasonic module, piezoelectric ceramics and an Archimedes spiral structure concave lens are arranged to generate the vortex sound field to act on a cell culture dish, so that an adjustable shear flow is generated on the cell culture dish, and the adherent cell is separated from the cell culture dish. And locally separating the adherent cells in the cell culture dish. According to the concave lens with the Archimedes spiral structure, the problem of defocusing of a high-order vortex sound field can be solved, and the falling control accuracy of adherent cells is improved. The proposed method does not require any physical contact with adherent cells, and does not require insertion of an instrument into a cell culture dish, thereby reducing the risk of contamination and cell damage. The stable flow generated by absorbing sound energy by the liquid is used for indirectly separating the adherent cells, the method is extremely low in energy density and has good biocompatibility, and the activity of the cells is ensured while the cells are efficiently separated.

The invention discloses an intelligent cooperative regulation and control method and system for dissolved oxygen in a haematococcus culture solution, relates to the field of bioengineering, and controls a decision-making layer to dynamically switch between a growth promotion regulation and control mode and a stress induction regulation and control mode based on biomass data and physiological status data in a haematococcus culture stage so as to realize cooperative regulation and control of dissolved oxygen, illumination, pH and a carbon source. According to the method, a growth promotion mode and a stress induction mode are dynamically switched, biomass accumulation is promoted through dissolved oxygen sine wave pulse fluctuation in the vegetative period, astaxanthin synthesis is induced through high-oxygen and low-oxygen circulation and blue light regulation in the stress period, and compared with traditional static regulation, the biomass is increased, and the yield of astaxanthin is increased. A light-oxygen synergistic factor k is introduced to dynamically adjust the fluctuation range of dissolved oxygen, the problem of illumination-dissolved oxygen mismatching is solved, and the photosynthetic efficiency is improved; a stress intensity index SI is established, stress parameters are adaptively optimized based on the astaxanthin synthesis rate, cell damage caused by over-stress is avoided, and the stability of the astaxanthin synthesis rate in the stress period is improved.

The invention relates to the technical field of cell separation and culture, in particular to a method for separating and culturing haematochrome cells of plectropomus leopardus. The separation method of the plectropomus leopardus haematochrome cells is created for the first time, the EDTA digestive juice is used for pre-digesting epidermis cells on the outer layers of the plectropomus leopardus fins, then the collagenase digestive juice is used for digesting connective tissues (pigmented cells) of internal dermis, digestive enzymes such as trypsin and other mechanical methods which have great damage to the cells are not used, and the method is suitable for large-scale production of the plectropomus leopardus haematochrome cells. The plectropomus leopardus haematochrome cell can reduce cell damage, can be effectively separated to obtain the plectropomus leopardus haematochrome cell which is high in purity, good in activity and capable of being cultured in vitro, and lays a foundation for subsequent experiments such as single cell sequencing, cell in-vitro culture and molecular function verification.

The cell storage device comprises a storage box, the top of the storage box is provided with a material taking and placing port used for taking and placing test tubes into the storage box, the two ends of the interior of the storage box are symmetrically and fixedly connected with a first vertical frame and a second vertical frame, and lifting grooves are formed in the first vertical frame and the second vertical frame; lifting plates are slidably arranged in the lifting grooves, a supporting shaft is fixedly connected between the two lifting plates, cross-shaped plates are fixedly connected to the two ends of the supporting shaft, and cell storage mechanisms are rotatably connected between the corresponding ends of the two cross-shaped plates through bearings; the device has the beneficial effects that through the synergistic effect of the rotary cell storage mechanism and the liquid nitrogen freezing system, cell samples in all test tubes are uniformly exposed in a low-temperature environment, cell damage caused by non-uniform local temperature is avoided, and when cells are taken and placed, the test tube mounting rack can be controlled to be lifted to the position of a material taking and placing opening, so that the test tubes are conveniently taken and placed. The target sample can be accurately stored and taken without integrally taking out the storage rack.

The invention provides a cryopreservation and resuscitation method for induced differentiation of pluripotent stem cells to metaphase cells and application, and the cryopreservation method comprises the following steps: (1) the initial cells are pluripotent stem cells, and are induced and differentiated to the metaphase; (2) recovering the cells by using a mild digestive enzyme, counting, and resuspending the cells in a cryopreservation solution; and (3) carrying out programmed cooling to-80 DEG C, and then transferring into liquid nitrogen for preservation for later use. According to the cryopreservation method and the cryopreservation liquid provided by the invention, the cryopreservation survival rate of the differentiated metaphase cells is obviously improved, apoptosis or irreversible stress injury induced by a traditional cryopreservation liquid is avoided, and the problems of large cell injury and low survival rate caused by using a general cryopreservation liquid in the prior art are obviously improved. The resuscitated cells keep good differentiation potential, can smoothly form a mature renal unit structure, and solves the problem of subsequent differentiation failure in the prior art.

The invention relates to a fused exosome-like vesicle based on stem cell exosomes and application thereof, and the fused exosome-like vesicle is prepared by a method comprising the following steps: mixing stem cell exosomes and aloe exosome-like vesicles under an ultrasonic condition, and incubating to obtain the fused exosome-like vesicle. The fused exosome-like vesicle can effectively promote percutaneous penetration of stem cell exosome, and can effectively improve cell damage caused by ultraviolet rays, regenerate and repair skin damage barriers such as basilar membranes and the like, resist inflammation and oxidation, effectively remove melanin, and improve the skin quality. The composition has good repair potentials for chloasma and skin damage, aging and melanin deposition related to chloasma. Sufficient data support and technical support are provided for research and development of stem cell exosome innovative drugs, and clinical application of the stem cell exosome innovative drugs is accelerated.

The invention belongs to the technical field of traditional Chinese medicine biology, and particularly relates to a phenolic diastereoisomer and a preparation method and application thereof.The phenolic diastereoisomer has the chemical structural formula shown in the formula I and the formula II, the formula I is dextroisomer and is named as (+)-fresh bamboo juice phenol D, and the formula II is levoisomer and is named as (-)-fresh bamboo juice phenol D. A pair of phenolic diastereoisomer compounds (+)-fresh bamboo juice phenol D and (-)-fresh bamboo juice phenol D are successfully obtained from fresh bamboo juice, and an anti-neurotoxicity activity test shows that (+)-fresh bamboo juice phenol D has a remarkable protection effect on PC12 cell injury caused by glutamic acid and can be used for preparing medicines for preventing or treating neurodegenerative diseases; wide application prospects are realized.

The invention discloses a radix gentianae callus cell culture and application method, and relates to the technical field of plant biology.The method comprises the following steps that in the laser irradiation process, a real-time spectrum analyzer is used for monitoring radix gentianae tissue, and absorption characteristic information of the plant tissue to light with different wavelengths is obtained. By combining the real-time spectral analysis and the machine learning model, the scheme can accurately monitor the interaction between the laser wavelength and the photosensitive component in the plant tissue, and by intelligently evaluating and dynamically adjusting the laser wavelength, the excessive absorption of laser energy is avoided, and the photochemical reaction and cell damage are reduced. The method not only reduces the potential damage of laser irradiation to plant cells, but also optimizes the synthesis process of medicinal components, and ensures the high efficiency, repeatability and reliability of callus culture. According to the scheme, powerful support is provided for improving the accuracy of experimental results and commercialized application of the gentiana scabra bunge.

The invention provides a composition for improving a barrier function and application thereof, and relates to the technical field of cell injury nursing. Gamma-aminobutyric acid (GABA) and cordyceps militaris fermentation filtrate (FCM) are used for treating barrier damage for the first time. Specifically, the combination of the gamma-aminobutyric acid and the cordyceps militaris fermentation filtrate can significantly improve damage of an epithelial mechanical barrier and an immune barrier caused by external stimulation, so that the epithelial barrier function can be prevented and improved from multiple dimensions.

The invention discloses application of a neutralizing antibody of oncostatin M in preparation of a medicine for treating bronchial pulmonary dysplasia. The application proves that the intrauterine inflammation can cause BPD-like lung lesion to the filial generation mouse, and the OSM expression level in the mouse lung tissue one day after the intrauterine inflammation filial generation is grown is obviously higher than that of a control group; the OSM level of the BPD group is higher than that of the non-BPD group in the umbilical blood sample of the premature infant; in-vitro experiments show that the OSM treatment can cause lung epithelial cell injury; by dripping a recombined OSM neutralizing antibody into the nose of a newborn mouse, down-regulation of the OSM level in the lung of the mouse is realized, filial generation lung injury induced by the intrauterine inflammation can be repaired, and the OSM can be used as a treatment target of the BPD caused by the intrauterine inflammation.

The invention provides processed rhizoma polygonati polysaccharide, a preparation method thereof and application of the processed rhizoma polygonati polysaccharide in preparation of a medicine for treating renal injury. The nine-processed polygonatum sibiricum polysaccharide is named as NPCP and is composed of fructose and glucose, the NPCP is straight-chain inulin type polysaccharide with the polymerization degree of 12, and the structure of the NPCP is Glcp-(1-) and Fruf-(2-terminal,-1)-Fruf-(2-main chain. According to the obtained uniform nine-process polygonatum polysaccharide NPCP, the protective effect on adenine-induced HK-2 cell injury is achieved by improving the content of renal function markers, improving oxidative stress injury, reducing expression of proinflammatory cytokines, improving intestinal flora disorder and up-regulating short-chain fatty acid in the intestinal tract; more treatment directions are provided for effectively improving adenine-induced renal toxicity by the nine-process polygonatum polysaccharide, and theoretical and experimental bases are provided for clinically finding effective preparation of drugs for treating renal injury.

The invention discloses a method for designing a bionic femoral stent based on fusion of a VT structure and a TPMS structure, and belongs to the technical field of bionic bone stents. The method comprises the following steps: respectively creating a Diamond unit homogeneous porous structure and a VT porous structure; and creating a Ramp fusion function, substituting the homogeneous porous structure of the Diamond unit and the Ramp fusion function into a Mix instruction, and then sequentially substituting the VT porous structures into the Mix instruction to generate a fusion model. The homogeneous porous structure of the Diamond unit and the VT porous structure are subjected to radial smooth fusion, smooth connection is realized at the fusion position, the service life of the porous structure serving as the bionic bone scaffold is shortened, the smooth surface of the D unit and the periodic structure uniformly conduct stress, the rounded Thiessen polygonal structure further disperses stress, and the stress dispersion and synergistic effects are achieved. The bionic femoral stent constructed by the invention can simulate natural bones, promote cell adhesion, proliferation and differentiation, and optimize transportation of nutrient substances and metabolic wastes. And the smooth fusion interface reduces cell damage and reduces immunological rejection.

The invention discloses a microfluidic image flow cytometry identification system and a microfluidic image flow cytometry identification method based on multi-modal phase imaging and deep learning, relates to a microfluidic image flow cytometry detection technology, and belongs to the crossing field of microfluidics, optical imaging and artificial intelligence. The cell deformation chip is used for inducing a cell sample to generate controllable deformation through the cell deformation chip and discharging deformed cells; the optical imaging unit is used for scanning the deformed cells and collecting multi-modal phase images of the deformed cells; the data processing and analysis unit is used for preprocessing the multi-modal phase image and performing cell intelligent identification analysis on the preprocessed multi-modal phase image by using a deep learning feature extraction network, so that accurate identification and classification of cells are realized, and a leukocyte subpopulation identification model is constructed; and generating a cell imaging result and a cell mechanical parameter thermodynamic diagram. According to the invention, label-free identification of leukocyte subgroups is realized, and the problems of tedious operation and cell damage of traditional fluorescence labeling are solved.

The invention discloses a neuroprotective peptide with anti-inflammatory and anti-oxidation effects and application of the neuroprotective peptide. The neuroprotective polypeptide is a polypeptide with any one of the following sequences: FPDFVYK and APDTRLF. The invention provides an antioxidant effect of the neuroprotective polypeptide in a PC12 cell injury model induced by hydrogen peroxide, and the neuroprotective polypeptide can be used for preparing antioxidant drugs. The invention provides an anti-inflammatory effect of the neuroprotective polypeptide in a PC12 cell injury model induced by hydrogen peroxide, and the neuroprotective polypeptide can be used for preparing anti-inflammatory drugs. The invention provides the influence of the neuroprotective polypeptide on the expression of pathways and neurotrophic factors in PC12 nerve cells. The neuroprotective polypeptide reduces the activity of acetylcholin esterase (AChE) and up-regulates the expression of neurotrophic factors so as to alleviate nerve cell damage and enhance memory, thereby achieving the purpose of preventing and treating neurodegenerative diseases.

The invention relates to the field of biological environmental protection, in particular to a heterotrophic nitrification immobilized engineering bacterium for efficient denitrification and a preparation method and application thereof.According to the heterotrophic nitrification immobilized engineering bacterium for efficient denitrification and the preparation method and application of the heterotrophic nitrification immobilized engineering bacterium, polyethylene glycol-glucan two-aqueous-phase concentration and trehalose protection liquid are adopted for synergistic effect, the bottleneck of cell damage caused by a traditional flocculation method can be avoided, trehalose molecules can wrap bacterial cells, and the bacterial cells can be effectively degraded; the immobilized particles can resist osmotic pressure impact and pollutant invasion, the survival rate of thalli is higher than 95%, so that the immobilized particles always keep ultrahigh denitrifying enzyme activity in various sewage environments, honeycomb-shaped pores are formed in an ethyl cellulose matrix, a microenvironment with a high specific surface area is provided for thalli attachment, and the built-in nano zeolite continuously and slowly releases Cu < 2 + > and Zn < 2 + > trace elements, so that the immobilized particles can be recycled. A thallus metabolism enzyme system is synergistically activated, and the denitrification pathway function is enhanced; the chitosan-polydopamine outer coating layer strongly captures the nitrogen-containing pollutants, so that the removal efficiency of the nitrogen-containing pollutants is improved; through triple protection, the density of unit granular bacteria is up to 109 cells / cm, the service life is prolonged, and the denitrification performance is improved by more than 40%.

The invention discloses a formula composition which is prepared from the following components in parts by weight: 100 to 400 parts by weight of grape seed procyanidine, 100 to 300 parts by weight of pomegranate polyphenol, 10 to 100 parts by weight of ergothioneine, 50 to 200 parts by weight of catechin and 100 to 400 parts by weight of astragalus polysaccharide. According to the invention, cell-level anti-aging is taken as a core logic, five active ingredients of grape seed procyanidine, pomegranate polyphenol, ergothioneine, catechin and astragalus polysaccharide are scientifically compounded, a multi-dimensional anti-oxidation defense system is constructed, and multiple effects of scavenging free radicals, repairing cell damage, delaying an aging process and the like can be realized.

The invention relates to the field of biomedical materials, in particular to an Nb2C MXene composite functional film capable of promoting angiogenesis and a preparation method of the Nb2C MXene composite functional film, the Nb2C MXene composite functional film is particularly suitable for diabetes wound healing, and the Nb2C MXene composite functional film comprises an Nb2C MXene functionalized collagen vitrified film layer, and a carboxymethyl chitosan vitrification film layer, wherein a nano interlocking interface is formed between the Nb2C MXene functionalized collagen vitrified membrane layer and the carboxymethyl chitosan vitrified membrane layer; the Nb2C MXene functionalized collagen vitrified membrane layer is hydrophobic, the carboxymethyl chitosan vitrified membrane layer is hydrophilic, through the double-layer asymmetric structural design, the inner layer is tightly attached to a wound, the outer layer prevents adhesion of bacteria and tissues, and the barrier function in the wound healing process is effectively guaranteed; the adopted Nb2CMXene has excellent ROS (reactive oxygen species) removal capacity, oxidative stress of wound parts can be effectively relieved, cell damage is avoided, and cell functions are recovered.

The invention belongs to the field of biological medicines, and particularly relates to application of a medicine for intervening in a mitochondrial pyruvic acid carrier in preparation of a medicine for treating acute pancreatitis and a medicine composition of the medicine. The medicine for intervening in the mitochondrial pyruvic acid carrier can reduce the serum enzyme level, relieve pancreatic pathological injury, inhibit inflammatory response, inhibit calcium overload and protect the mitochondrial function, so that pancreatic acinus cell injury is relieved, the effect of treating acute pancreatitis is achieved, and the medicine has a wide application prospect. The invention provides a new choice for clinically treating acute pancreatitis, and has a good application prospect.

The invention provides a cell magnetic sorting column and a preparation method thereof, and belongs to the technical field of cell magnetic sorting, the method comprises the following steps: S1, pretreating balls; s2, coating the surface of the ball with a thermoplastic high polymer material layer by adopting an electro-deposition process to form magnetic matrix particles; s3, sequentially putting a lower-layer filter screen, magnetic matrix particles and an upper-layer filter screen into the bottom shell of the separation column; s4, the upper sealing cover and the bottom shell are connected through ultrasonic welding; s5, centrifuging the sorting column subjected to ultrasonic welding; and S6, curing the centrifuged separation column. The cell magnetic separation column and the preparation method thereof are particularly suitable for magnetic separation of nanoscale magnetic beads, the problems that a traditional separation column is high in magnetic bead wastage rate, uneven in magnetic field, prone to blockage, prone to cell damage and the like can be solved, a high-purity and high-activity cell source is provided for cell therapy such as CAR-T, and the cell magnetic separation column is suitable for magnetic separation of nanoscale magnetic beads. And the process is simple and suitable for large-scale standardized production.

A laser assembly for controlling foliage growth including a body adapted to being mounted at a location in order to emit an axial direction defining laser pattern to establish a growth limiting boundary for any of a tree, shrub or like foliage. The laser pattern provides a high intensity light beam causing cellular damage to tissue of the foliage in order to retard growth into the affected area. The body further includes at least one eyepiece for issuing the laser pattern according to any of a pin-point or arcuate / sweep pattern. A clamp or post arrangement mounts the body in a multi-axial adjustable fashion.

The invention relates to the field of natural medicinal chemistry, and discloses an efficient separation and preparation method of a compound theanine diglucoside TFG. Although TFG structures are reported, separation from tea leaves is not disclosed. According to the invention, an efficient separation and purification method is established for the first time, and TFG is accurately extracted and identified from tea leaves by combining an HPLC-ELSD (High Performance Liquid Chromatography-Evaporative Light Scattering Detector) guided multi-stage column chromatography technology with nuclear magnetic resonance and mass spectrometry. Experiments prove that the TFG activates an antioxidant pathway, improves H2O2-induced HUVEC cell and HT22 cell damage, protects the vascular endothelial barrier function and neuronal activity, and is suitable for developing antioxidant drugs for treating Alzheimer's disease. Compared with the prior art, the invention fills the blank of separation of the tea-derived TFG, explores the new application of the tea-derived TFG in the field of cell protection, and provides a new thought for natural product development and functional food research.

The application discloses a kind of acanthopanax acid polysaccharides with intestinal protection function and its preparation method and application, belong to the field of functional food and biotechnology.The acanthopanax acid polysaccharide is by water extraction alcohol precipitation, after impurity removal, through DEAE-52 anion exchange column elution and collection 0.2 mol / L NaCl elution peak, and the uniform component obtained by Sephadex G-100 gel column chromatography purification.Its uronic acid content is 30%-40%, weight average relative molecular weight is 50-100kDa, mainly by glucose, galactose, galacturonic acid and arabinose, infrared spectrum has carboxyl characteristic absorption peak at 1736 cm-1 and 1615 cm-1.Experiments prove that the acanthopanax acid polysaccharide has antioxidant activity and hypoglycemic activity.The acid polysaccharide has protective effect on LPS-induced Caco-2 cell damage, can effectively reduce the secretion of pro-inflammatory factors TNF-α, IL-1β, IL-6, and improve the level of anti-inflammatory factor IL-10.The acanthopanax acid polysaccharide can be prepared into oral liquid, tablet candy, solid beverage and other functional food forms.

The application discloses application of skin precursor cell induced Schwann cell derived vesicles (SKP-SC-EVs) in preparation of a product for treating Parkinson's disease, wherein the SKP-SC-EVs have a protective effect in rotenone or MPP+-induced cell injury, and in a MPTP-induced mouse Parkinson's disease model, the SKP-SC-EVs can be given by a nasal instillation method to improve the motor ability of the mouse and restore the olfactory function, and the product can be used for research and treatment of Parkinson's disease.

The invention discloses novel application of ganoderic acid B, belongs to the field of liver injury treatment, and particularly relates to application of ganoderic acid B in preparation of a medicine for treating liver cell injury caused by aflatoxin. According to the invention, human liver cancer HepG2 cells are utilized to construct an AFB1 cell injury model, lucid ganoderma active ingredients with a protection effect on injury are screened out, and the resistance mechanism of the lucid ganoderma active ingredients is researched and predicted, so that a new method and a new target spot are provided for searching for diseases caused by AFB1 prevention and treatment. A new method can be provided for preventing and treating the liver cancer caused by the AFB1.

This invention relates to the fields of biomedical technology and biological model construction technology, and discloses an ANCA-associated vasculitis glomerular endothelial cell injury model, its construction method and application, and the application of BACH1 inhibitors. The method includes: (1) activating neutrophils sequentially with tumor necrosis factor α and immunoglobulin G to obtain a culture medium containing activated neutrophils; wherein the immunoglobulin G is immunoglobulin G isolated and purified from the plasma of patients with ANCA-associated vasculitis; (2) co-culturing the culture medium containing activated neutrophils with human glomerular endothelial cells to obtain the cell injury model. The cell injury model provided by this invention can verify the role of neutrophil adhesion characteristics in endothelial cell injury, and at the same time, the cell injury model can better simulate the immune microenvironment of ANCA-associated vasculitis glomerular endothelial cells in vivo.

A method for increasing or improving nitric oxide signals or levels, the method comprising: administering a composition to a subject, the composition comprising: arginine or a pharmaceutically acceptable salt, ester, or derivative thereof; and tetrahydrocurcumin and / or β-aminoisobutyric acid or a pharmaceutically acceptable salt or ester thereof. The method and the composition can be used to increase, improve, or enhance nitric oxide signals or levels, inhibit nitric oxide decomposition, and increase or enhance nitrous acid and / or cyclic guanosine monophosphate levels, and can improve fatigue resistance or enhance muscle strength, maintain or improve cardiovascular health, maintain or improve vascular function and structure, inhibit or mitigate endothelial cell damage, or mitigate insufficient blood flow.

The invention provides a cell activity detection method based on fluorescence labeling, and relates to the technical field of cell analysis. The cell activity detection method based on fluorescence labeling comprises the following steps: S1, co-incubating a cell sample to be detected with a mixed solution of propidium iodide, a JC-1 mitochondrial membrane potential dye, a DCFH-DA active oxygen probe, a Caspase-3 / 7 activated substrate and a Hoechst nuclear dye, completing synchronous multiple labeling, and generating a multi-labeled cell sample, S2, detecting the cell activity based on the multi-labeled cell sample, five-channel full-automatic scanning is carried out through a high-content imaging system, Z-stack images are collected, and a multispectral image data set is generated. The five probes are mixed and incubated at one time through a synchronous multiple labeling process, so that the operation time is remarkably shortened, and the risk of cell injury caused by multiple times of centrifugation is reduced; z-stack full-automatic scanning is combined with three-dimensional image acquisition, the capturing capability of weak fluorescence signals is improved through multi-focal plane fusion, and information loss caused by cell thickness or position offset in traditional two-dimensional imaging is overcome.