8 results about "Gelatinase" patented technology

The invention relates to the field of food, in particular to probiotic characteristics of lactobacillus salivarius JCF5 and a method for preparing Jersey yogurt through synergistic fermentation of the lactobacillus salivarius JCF5. The strain provided by the invention is named as Lactobacillus salivarius JCF5, and the Latin is Ligillactobacillussavarius. The invention further discloses a preparation method of the strain. The strain provided by the invention has the advantages that the strain is named as Lactobacillus salivarius JCF5; the strain is obtained by randomly selecting six newly-born Jersey and six Jersey-and-cattle hybrid calves with good health conditions from a pasture in Heilongjiang province, collecting fetal manure of the Jersey and six Jersey-and-cattle hybrid calves after the Jersey and cattle hybrid calves are born, and separating manure samples after the Jersey and cattle hybrid calves eat colostrum. The water-holding capacity and whey precipitation rate of the Jersey yoghourt can be remarkably improved, and the viscosity of the Jersey yoghourt can be increased. In addition, the strain has better acid resistance, cholate resistance and simulated gastrointestinal fluid resistance, does not show hemolysis capacity and gelatinase activity, has stronger antibacterial capacity and antibiotic sensitivity, and has the characteristics of probiotics. The application potential of the strain in fermenting the Jersey yogurt is fully explored, the quality of the Jersey yogurt is expected to be improved by changing the strain proportion of the leavening agent sold in the market, and a new leavening agent is provided for fermentation of the Jersey yogurt.

This invention relates to the field of bioengineering technology, specifically to a method for preparing neutrophil gelatinase-associated lipid transport protein and its antibody. Specifically, using pET23a(+) as the expression vector, the NGAL gene with six His tags fused to its N-terminus under promoter control is introduced into *E. coli* for induced expression. After purification using a Ni Focurose 6FF column and a molecular sieve column, tag-free NGAL protein with a purity ≥95% is obtained. Subsequently, the NGAL protein is used to repeatedly immunize Japanese rabbits, and serum is collected after each immunization. Qualified serum is screened using a two-dimensional agar diffusion assay, and then purified using the aforementioned antigen to prepare an antigen affinity purification column, yielding a highly active NGAL polyclonal antibody. The high-purity recombinant NGAL of this invention not only possesses activity but is also low-cost, not limited by raw materials, and can be mass-produced, possessing potential and wide-ranging application value in clinical and in vitro diagnostics.

The application belongs to the technical field of protein detection, and particularly relates to a protein marker detection platform based on a DNA nanoflower cascade CRISPR system and a construction method and application thereof. The protein marker detection platform based on the DNA nanoflower cascade CRISPR system comprises an upstream module of protein marker input signal amplification based on a DNA nanoflower and a downstream module of output signal amplification based on a CRISPR / Cas system. When the detection platform is used for protein marker detection, the upstream module based on the DNA nanoflower can convert the protein marker into hundreds or thousands of DNA signals, the DNA signals are recognized and activated by a Cas-crRNA compound to have Cas protein cis and trans cleavage activity, so that the output signal is amplified, high sensitivity and rapid detection of the protein marker are achieved, and in particular in detection of a neutrophil gelatinase lipocalin marker in a kidney injury disease, the detection limit is 500 fg / ml.

The invention discloses a biomarker composition and application thereof, and belongs to the field of biomarker analysis and detection. The invention firstly provides a group of biomarker compositions, wherein the biomarker compositions comprise a Klotho protein and a neutrophil gelatinase associated lipocalin (NGAL); the combined detection biomarker composition can be used for early assessment of the risk of hypertensive disorder in pregnancy complicated with acute kidney injury. When the marker composition is used for predicting the risk of hypertensive disorder in pregnancy complicated with acute kidney injury, the risk of acute kidney injury can be recognized in advance in the early pregnancy stage.

The invention discloses anti-oxidation active coating liquid capable of delaying freezing of a cooked food source as well as a preparation method and application of the anti-oxidation active coating liquid, and belongs to the technical field of food packaging materials. The anti-oxidation active coating liquid for delaying the frozen cooked food source comprises the following components: glycerol, water, sodium alginate and alkaline protease zymolyte of gelatin. According to the invention, gelatin zymolyte with specific activity is screened by using an enzymolysis technology, and sodium alginate is combined to obtain the food-source-based composite coating capable of effectively protecting food freshness, reducing ice crystal sublimation and inhibiting fat oxidation in a low-temperature environment. The anti-oxidation active coating capable of delaying frozen cooked food source base provided by the invention can effectively solve the problem of frozen cooked food caused by ice crystal sublimation, and is suitable for fresh keeping of frozen food such as meat and aquatic products.

The invention relates to a biomedical functional material and nano-drug delivery technology, in particular to dual-response nano-hydrogel for promoting healing of chronic wound surfaces of diabetes mellitus, a preparation method and application. According to the hydrogel, chitosan / sodium tripolyphosphate / hyaluronic acid nanoparticles are constructed to stably deliver miR-144-5p, oxymatrine is synergistically entrapped in a gelatin-TG enzyme cross-linked hydrogel matrix, in-situ gelling under the body temperature condition is achieved, and difunctional drugs are sequentially released. Animal experiments prove that the composition can significantly accelerate wound closure, improve VEGF and CD31 expression and reconstruct a dermal vascular network, has good biocompatibility and controlled release performance, and has wide clinical application prospects.

The present application relates to biomedical functional materials and nano drug delivery technology, and particularly relates to a double-response nano hydrogel for promoting chronic wound healing of diabetes, a preparation method and application. The hydrogel is stable in delivery of miR-144-5p by constructing chitosan / sodium tripolyphosphate / hyaluronic acid nanoparticles, and is synergistically loaded with oxymatrine in a gelatin-TG enzyme crosslinking hydrogel matrix, so that in-situ gelation and sequential release of double functional drugs are realized under body temperature. Animal experiments prove that the composition can significantly accelerate wound closure, improve VEGF and CD31 expression and reconstruct dermal vascular network, and has good biocompatibility and controlled release performance, and has a wide clinical application prospect.

The invention discloses a method for preparing a bone gelatin composite antialcoholism peptide based on enzymolysis and fermentation technologies, and belongs to the technical field of antialcoholism peptide preparation. Bone gelatin and ultrapure water are mixed and swelled, then enzymolysis is conducted, and bone gelatin enzymatic hydrolysate is obtained after enzymolysis is completed; food-grade galactooligosaccharide powder is dissolved in the chicory root water extract, and a composite prebiotic solution is obtained; the mixed herbal powder is put into a composite prebiotic solution, food-grade ethanol is added after extraction, and an ethanol stress induced pretreated herbal synergistic solution is obtained; and mixing the bone gelatin enzymatic hydrolysate with the herbal synergistic liquid subjected to ethanol stress induction pretreatment to obtain a mixed liquid, fermenting, centrifuging, and freeze-drying to obtain the bone gelatin composite anti-alcoholism peptide. Through the mode, the ADH activation rate, the ALDH activation rate and the DPPH clearance rate of the dealcoholism peptide are improved.