4 results about "Gelatinase" patented technology

This invention relates to the field of bioengineering technology, specifically to a method for preparing neutrophil gelatinase-associated lipid transport protein and its antibody. Specifically, using pET23a(+) as the expression vector, the NGAL gene with six His tags fused to its N-terminus under promoter control is introduced into *E. coli* for induced expression. After purification using a Ni Focurose 6FF column and a molecular sieve column, tag-free NGAL protein with a purity ≥95% is obtained. Subsequently, the NGAL protein is used to repeatedly immunize Japanese rabbits, and serum is collected after each immunization. Qualified serum is screened using a two-dimensional agar diffusion assay, and then purified using the aforementioned antigen to prepare an antigen affinity purification column, yielding a highly active NGAL polyclonal antibody. The high-purity recombinant NGAL of this invention not only possesses activity but is also low-cost, not limited by raw materials, and can be mass-produced, possessing potential and wide-ranging application value in clinical and in vitro diagnostics.

The invention discloses a biomarker composition and application thereof, and belongs to the field of biomarker analysis and detection. The invention firstly provides a group of biomarker compositions, wherein the biomarker compositions comprise a Klotho protein and a neutrophil gelatinase associated lipocalin (NGAL); the combined detection biomarker composition can be used for early assessment of the risk of hypertensive disorder in pregnancy complicated with acute kidney injury. When the marker composition is used for predicting the risk of hypertensive disorder in pregnancy complicated with acute kidney injury, the risk of acute kidney injury can be recognized in advance in the early pregnancy stage.

The present application relates to biomedical functional materials and nano drug delivery technology, and particularly relates to a double-response nano hydrogel for promoting chronic wound healing of diabetes, a preparation method and application. The hydrogel is stable in delivery of miR-144-5p by constructing chitosan / sodium tripolyphosphate / hyaluronic acid nanoparticles, and is synergistically loaded with oxymatrine in a gelatin-TG enzyme crosslinking hydrogel matrix, so that in-situ gelation and sequential release of double functional drugs are realized under body temperature. Animal experiments prove that the composition can significantly accelerate wound closure, improve VEGF and CD31 expression and reconstruct dermal vascular network, and has good biocompatibility and controlled release performance, and has a wide clinical application prospect.

The invention discloses a method for preparing a bone gelatin composite antialcoholism peptide based on enzymolysis and fermentation technologies, and belongs to the technical field of antialcoholism peptide preparation. Bone gelatin and ultrapure water are mixed and swelled, then enzymolysis is conducted, and bone gelatin enzymatic hydrolysate is obtained after enzymolysis is completed; food-grade galactooligosaccharide powder is dissolved in the chicory root water extract, and a composite prebiotic solution is obtained; the mixed herbal powder is put into a composite prebiotic solution, food-grade ethanol is added after extraction, and an ethanol stress induced pretreated herbal synergistic solution is obtained; and mixing the bone gelatin enzymatic hydrolysate with the herbal synergistic liquid subjected to ethanol stress induction pretreatment to obtain a mixed liquid, fermenting, centrifuging, and freeze-drying to obtain the bone gelatin composite anti-alcoholism peptide. Through the mode, the ADH activation rate, the ALDH activation rate and the DPPH clearance rate of the dealcoholism peptide are improved.