47 results about "Neutrophil gelatinase-associated lipocalin" patented technology

The invention discloses a neutrophil gelatinase associated lipocalin (NGAL) chemiluminescence detection kit. The kit comprises the components of a standard substance of NGAL, an NGAL monoclonal antibody labeled by horseradish peroxidase, magnetic particles coated by the NGAL monoclonal antibody, a white non-transparent microwell plate, washing liquid, and chemiluminescence substrates A and B. The kit provided by the invention can be used for detecting the content of the NGAL in blood serum of a patient, and has important guiding significance to the detections on premature kidney diseases and injures. Compared with the conventional ELISA (Enzyme Linked Immunosorbent Assay) method, the NGAL chemiluminescence detection kit provided by the invention has the characteristics of being high in sensitivity and good in specificity and overcoming the large influence of immunoturbidimetry by blood fat concentration, and has the advantages of high stability, reliability and accuracy, security and environment friendliness, simplicity and convenience in operation and the like.

The invention discloses a detection kit for neutrophil gelatinase-associated lipocalin. The detection kit comprises a detection liquid and a standard substance, wherein the detection liquid comprises latex particles coupled with an anti-human neutrophil gelatinase-associated lipocalin antibody; the standard substance is naturally configured recombinant neutrophil gelatinase-associated lipocalin. The detection kit measures the content of the neutrophil gelatinase-associated lipocalin through a latex particle intensified immunity turbidity, and compared with the traditional NGAL detection kit, the detection kit has the advantages of high detection sensitivity, high simpleness in operation, good repeatability and short time consumption, does not need sample pretreatment, and can be used for a fully automatic biochemical analyzer.

The invention relates to a method for preparing an NGAL (Neutrophil Gelatinase Associated Lipocalin) optical excitation chemiluminescence detection kit. The method comprises the following steps: activating a luminous microsphere, namely activating the luminous microsphere through prepared carbodiimide and Sulfo-NHS solutions in a PBS (Phosphate Buffer Saline) buffer solution, wherein the luminous microsphere is a carboxyl modified luminous microsphere, and the luminous microsphere is coated with dimethylthiophene, anthracene and rubrene and carried with an europium chelate; coupling the luminous microsphere with an anti-NGAL antibody, namely selecting the carboxyl modified luminous microsphere, carrying out mixing reaction on the anti-NGAL monoclonal antibody and the activated carboxyl modified luminous microsphere to couple the anti-NGAL antibody to the luminous microsphere, and adding freshly prepared carbodiimide at the same time while mixing the anti-NGAL antibody and the activated luminous microsphere; labeling the anti-NGAL antibody through biotin; and labeling a photosensitive microsphere through avidin. The invention also relates to preparation and use methods of the NGAL optical excitation chemiluminescence detection kit. The methods disclosed by the invention solve the problems that the sensitivity is low and the detection is easily interfered by the environment in a detection method in the prior art.

The invention relates to a novel reagent and a kit for renal injury monitoring. The inventor identifies critical antigenic determinants from full-length NGAL (neutrophil gelatinase-associated lipocalin). And a short peptide mixture containing the critical antigenic determinants is adopted as an immunogen to obtain a specific polyclonal antibody against NGAL. The antigen fragments and its polyclonal antibody provided in the invention have the advantages of simple preparation method, high titer, strong specificity, and high sensitivity.

This invention belongs to the field of biotechnology detection, particularly relates to a triple kit for early warning, treatment process and monitoring after treatment of renal failure, and further discloses a preparation method and application thereof. Serum cystatin (CysC), neutrophil gelatinase-associated lipocalin (NAGL) and C reactive protein (CRP) are used as detecting markers. The triple kit uses NGAL-CysC-CRP triple antibody as the markers, can effectively monitor the risk of renal failure, especially plays an unexpected effect of monitoring of acute renal failure of persons subjected to a traffic accident, and is high in diagnosis sensitivity of acute renal failure, and strong in specificity, the clinical monitoring accuracy is as high as 95%, and the triple kit can make nephropathy self testing and monitoring of persons with diabetes possible, is used for kidney disease early warning, can timely prevent deterioration of the patient's condition, and reduces the patient's degree of kidney damage.

The present invention relates to the use of Neutrophil Gelatinase-Associated Lipocalin (NGAL) and / or SERPINA3 as biomarkers of the Mineralocorticoid Receptor (MR) activation in a patient. More particularly, the present invention relates to a method for predicting the responsiveness of a patient to a treatment with a MR antagonist or an aldosterone synthase inhibitor, said method comprising determining in a biological sample obtained from said patient the expression level of the NGAL gene and / or of the SERPINA3 gene.

A method is provided of diagnosing and monitoring acute renal injury leading to acute renal failure in a human or mammalian subject by determining the ratio of the concentration of neutrophil gelatinase-associated lipocalin (NGAL) in urine to that in plasma or serum.

The invention relates to a kit for detecting acute kidney injury. The kit comprises a first detection liquid and a second detection liquid, wherein a first anti-NGAL (neutrophil gelatinase-associated lipocalin) monoclonal antibody coated with magnetic particles is contained in the first detecting liquid, and a second anti-NGAL monoclonal antibody labeled with a chemiluminescent label is contained in the second detection liquid. The first anti-NGAL monoclonal antibody and the second anti-NGAL monoclonal antibody are used for different NGAL epitopes respectively. The kit for detecting acute kidney injury is used for detection on basis of NGAL serving as a marker for diagnosis detection, can rapidly detect and diagnose the acute kidney injury at the early stage and is higher in sensitivity and specificity of detection.

The invention relates to a kit for quantitatively detecting NGAL in urine or blood, comprising a box cover, a box body, a first reagent unit and a second reagent unit placed in the box body, and the The outside is independent of each other. The first reagent unit and the second reagent unit are connected by a bracket, and the two reagent units connected by the bracket are filled with fixatives. The reagents contained in the first reagent unit include phosphate buffer saline, triton, polyethylene glycol Alcohol, sodium azide, the reagents contained in the second reagent unit include latex particles modified by mouse anti-human NGAL monoclonal antibody, latex particles modified by goat anti-human NGAL polyclonal antibody, casein, and sodium azide. The invention is easy to use and operate, can quickly and accurately quantitatively detect NGAL in urine or blood, improves detection sensitivity and linear measurement range, and fully meets clinical detection requirements.

The invention discloses a kit for detecting the content of neutrophil gelatinase-associated lipocalin (NGAL). The kit comprises a reagent R1, a reagent R2 and an NGAL reagent reference standard substance, wherein the reagent R1 comprises the following components: 10mM-100mM of Tris, 50mM-200mM of NaCl, 0.05%-1% of BSA (Bovine Serum Albumin), 0.01%-0.1% of Tween-20, 0.5%-3% of PEG (Polyethylene Glycol) and 0.1% of NaN3; the reagent R2 comprises the following components: 10mM-100mM of Tris, 50mM-200mM of NaCl, 0.05%-1% of BSA, 0.01%-0.1% of Tween-20, 0.1% of NaN3, 1%-10% of saccharose and 0.1%-1% of NGAL antibody sensitization latex particles; the NGAL reagent reference standard substance comprises the following components: 10mM-100mM of Tris, 50mM-200mM of NaCl, 0.05%-1% of BSA, 0.01%-0.1% of Tween-20, 0.5mM-5mM of EDTA (ethylenediamine tetraacetic acid) and 0.1% of NaN3. The kit is simple in reagent composition, high in test sensitivity, wide in linear range, high in stability, low in test cost, high in accuracy and convenient to popularize.

Methods for the detection of active lupus nephritis (LN) and worsening renal disease activity and / or active LN in patients diagnosed with systemic lupus erythematosus, using a panel of biomarkers including transferrin (Tf), ceruloplasmin (Cp), alpha-1-acid glycoprotein (AGP1), lipocalin-like prostaglandin D synthetase (L-PGDS), and urinary neutrophil gelatinase associated lipocalin (UNGAL).