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Kit for detecting acute kidney injury

A detection kit, a technology for acute kidney injury, which is applied in the field of detection kits for acute kidney injury, and can solve the problems of poor specificity and low sensitivity

Inactive Publication Date: 2017-12-29
FAPON BIOTECH INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

ELISA has low sensitivity and is mainly qualitative detection
[0007] Traditional detection kits for acute kidney injury based on NGAL as a diagnostic marker have low sensitivity and poor specificity

Method used

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  • Kit for detecting acute kidney injury
  • Kit for detecting acute kidney injury
  • Kit for detecting acute kidney injury

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Experimental program
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Effect test

Embodiment 1

[0066] The acute kidney injury detection kit includes a first detection solution, a second detection solution, a substrate solution and NGAL standard products. Wherein the first detection solution contains the first anti-NGAL monoclonal antibody coated with magnetic particles at a concentration of 0.5 mg / mL (the mass ratio of the first anti-NGAL monoclonal antibody to magnetic particles is 0.05:1), and the concentration is 50 mmol / L HEPES (4-hydroxyethylpiperazineethanesulfonic acid), 1% BSA by mass fraction, 1% sodium chloride (NaCl) by mass fraction, 5% sucrose by mass fraction, 0.1% by mass fraction Tween-20 and PC-300 with a mass fraction of 0.1%, the rest of ultrapure water, and the pH value of the first detection solution is 7.0. The second detection solution contains HRP-labeled second anti-NGAL monoclonal antibody at a concentration of 1 μg / mL (the molar ratio of HRP to the second anti-NGAL monoclonal antibody is 1:1), and Tris-HCl at a concentration of 50 mmol / L. , m...

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Abstract

The invention relates to a kit for detecting acute kidney injury. The kit comprises a first detection liquid and a second detection liquid, wherein a first anti-NGAL (neutrophil gelatinase-associated lipocalin) monoclonal antibody coated with magnetic particles is contained in the first detecting liquid, and a second anti-NGAL monoclonal antibody labeled with a chemiluminescent label is contained in the second detection liquid. The first anti-NGAL monoclonal antibody and the second anti-NGAL monoclonal antibody are used for different NGAL epitopes respectively. The kit for detecting acute kidney injury is used for detection on basis of NGAL serving as a marker for diagnosis detection, can rapidly detect and diagnose the acute kidney injury at the early stage and is higher in sensitivity and specificity of detection.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a detection kit for acute kidney injury. Background technique [0002] Acute kidney injury (acute kidney injury, AKI) is a clinical syndrome caused by a sudden decline in renal function in a short period of time (hours to days) caused by various reasons, and is a common disease that threatens the lives of critically ill patients. Usually, more than 50% of patients in intensive care have different degrees of kidney damage, and the mortality rate of acute renal failure is at the forefront of the mortality rate of critical illness. In the past few decades, people's understanding of the pathogenesis of AKI and the level of medical technology have been greatly improved, but the morbidity and mortality of AKI are still high. One of the main reasons leading to this situation is the lack of effective early diagnostic markers. Traditional diagnostic indicators (serum creatinine sCr, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/92G01N33/58G01N33/577G01N33/543
CPCG01N33/92G01N33/54326G01N33/577G01N33/58G01N2800/347
Inventor 李瑞净刘俊鹏胡金灵阳馨滢
Owner FAPON BIOTECH INC
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