22 results about "Common disease" patented technology

The invention belongs to the technical field of biology, and discloses a TaqMan primer probe combination for detecting virulent and attenuated Newcastle disease, which comprises a virulent primer RT, a virulent probe RT-probe, a universal primer SRT and a universal probe SRT-probe, the virulent primer RT comprises an upstream primer RT-F and a downstream primer RT-R, and the universal primer SRT comprises an upstream primer SRT-F and a downstream primer SRT-R; a TaqMan probe fluorescent quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection method for virulent and attenuated NDV (Newcastle Disease Virus) established by using the TaqMan primer probe combination has no cross reaction on amplification of common avian viruses AIV, FADV, IBV and IBDV and has good specificity; the lowest detection concentration of virulent and attenuated Newcastle disease virus is 101 copies / uL, which is 100 times higher than that of a conventional RT-PCR method, and the kit has good sensitivity; and compared with the conventional RT-PCR method, the detection rate is 100% and is superior to that of the conventional RT-PCR method, and the detection result is consistent with the sequencing result.

The invention relates to the technical field of molecular biology. The invention provides a primer probe combination for detecting an H10 subtype avian influenza virus, the primer probe combination comprises a primer pair and a probe for detecting the H10 subtype avian influenza virus, and the primer pair for detecting the H10 subtype avian influenza virus comprises an H10-F sequence as shown in SEQ ID NO.1 and an H10-R sequence as shown in SEQ ID NO.2; and the probe for detecting the H10 subtype avian influenza virus is an H10-P sequence as shown in SEQ ID NO.3. The invention further discloses a kit for detecting the H10 subtype avian influenza virus. The primer probe combination disclosed by the invention can be used for detecting and distinguishing epidemic H10 viruses of European subpedigree and North American pedigree, does not generate cross reaction with other common viruses, and is high in detection sensitivity, and the minimum detectable quantity is 5 copies.

The invention relates to the technical field of biological detection, relates to a dual RT-ddPCR detection method for equine encephalomyelitis viruses in the eastern and western parts, and in particular relates to a monitoring and quantitative detection method for samples with relatively low equine encephalomyelitis virus content or complex matrix, such as feeds, animal tissues and animal-derived products. The double reverse transcription microdroplet digital PCR detection method for the equine encephalomyelitis virus, established by the invention, is strong in specificity, high in sensitivity, good in repeatability and strong in anti-interference capability, and can be used for absolutely quantifying the copy concentration of the eastern equine encephalomyelitis virus EEEV and the western equine encephalomyelitis virus WEEV in an actual sample; a novel and reliable technical method is provided for early trace early warning and monitoring of Eastern equine encephalomyelitis virus EEEV and Western equine encephalomyelitis virus WEEV infection, differential diagnosis of other common viruses, research of propagation rules of the viruses, epidemiological risk assessment and other systematic applications.

The invention discloses a respiratory tract pathogen PCR-HRM detection kit and application thereof, and belongs to the technical field of detection, and the kit comprises primer probes as shown in SEQ ID No.1-90. Based on a PCR-HRM (Polymerase Chain Reaction-High Resolution Melting) method, the detection kit for detecting the 26 common pathogens of the respiratory tract syndrome, established by the invention, has the detection sensitivity of 100 Copies / mL (preferably 50 Copies / mL), and can meet the expected requirement of monitoring the pathogens at a port. In the correctness verification process, compared with a tNGS detection result, the conformity of the detected target species is not lower than 96.1%, which indicates that the method has high reliability and accuracy in pathogen detection.

The application discloses a disease recognition method and device based on multi-modal fusion, comprising the following steps: multi-modal feature extraction processing is performed on multi-modal original data, and quality and confidence of the multi-modal features are evaluated; based on an attention mechanism, dynamic weight generation processing is performed on the quality and confidence evaluation results of the multi-modal features, and adaptive feature fusion is performed on the features of each mode; first-stage preliminary screening processing is performed on the adaptive feature fusion results, and a heat map of an unusual case abnormal area or a common case diagnosis result is output; second-stage refined diagnosis is performed on the first-stage preliminary screening processing results in combination with a medical knowledge graph, and a refined diagnosis report is generated; in the first diagnosis stage, rapid screening positioning is realized, and in the second diagnosis stage, suspicious problems are analyzed in depth, so that it is explained which data are used for diagnosis, where the screening positioning result of the first diagnosis stage is observed, and what the second diagnosis stage identification reason is.

The application discloses a MIRA-LFD primer probe composition for visual rapid detection of DHAV-1 and DHAV-3, a kit and application thereof. The primer probe composition comprises a first primer pair and a probe for detecting DHAV-1, and a second primer pair and a probe for detecting DHAV-3. The application is based on a multi-enzyme constant temperature rapid amplification technology combined with a lateral flow chromatographic test strip, and visual double detection of DHAV-1 and DHAV-3 can be completed within 15 min under a constant temperature condition of 39 DEG C, and the minimum detection limit is 1.5 x 10 2 copies / muL, and there is no cross reaction with other common viruses of duck origin. The primer probe composition and the kit are used for detecting clinical samples, and the result is consistent with that of an RT-PCR method, and the primer probe composition and the kit have the advantages of simple operation, rapidness, and no need of expensive instruments.

The invention discloses a knowledge graph-based intelligent auxiliary diagnosis and treatment system for grassroots medical common diseases, and relates to the technical field of medical care informatics, and the system comprises the following steps: 1, obtaining a medical record text of a patient, and taking a patient element anchoring node set as a starting point to execute subgraph traversal to extract candidate diagnosis and treatment subgraphs; 2, extracting patient observation facts and diagnosis knowledge facts from the candidate diagnosis and treatment subgraphs to form a diagnosis logic fact set, and arranging the diagnosis logic fact set in a descending order according to comprehensive evidence intensity to form candidate disease reasoning results; and step 3, coding clinical diagnosis and treatment path specifications into a fuzzy Petri network, and summarizing to generate an intelligent auxiliary diagnosis and treatment result. According to the method, the interpretability of logical reasoning, the fuzzy matching capability of semantic embedding and the differentiated path constraint of the fuzzy Petri network are organically fused, and a standardized and interpretable intelligent auxiliary diagnosis and treatment result can be provided under the condition that basic medical diagnosis evidence is incomplete and sufficient.

The invention discloses a primer probe composition for detecting AIV, NDV virulent strains and IBV, a kit and application, and belongs to the technical field of fluorescence detection. The sequence of the primer probe combination is shown as SEQ ID NO.1-SEQ ID NO.9, the primer probe combination comprises a primer pair and a probe for detecting the avian influenza virus, the Newcastle disease virus virulent strain and the avian infectious bronchitis virus, and the primer probe combination can be used for detecting and distinguishing all subtypes of the avian influenza virus and the Newcastle disease virus virulent strain and different genotypes of the avian infectious bronchitis viruses. The kit is high in specificity and sensitivity, does not have cross reaction with other common viruses, and is high in detection sensitivity, and the minimum detectable quantity of the three viruses is 0.8 copy, so that the specific, sensitive and rapid detection of AIV, NDV and IBV can be realized, and the defects of the conventional detection technology are overcome. The method has important practical significance on timely blocking epidemic situation propagation, reducing breeding loss, guaranteeing public health safety and the like.

The present application relates to the technical field of virus detection, in particular to a primer probe combination for identifying mixed infection pathogens of cattle and application and kit thereof. When identifying cattle pathogens, the nucleotide sequence of the upstream primer of the primer probe combination used is shown in SEQ ID No. 1, the nucleotide sequence of the downstream primer is shown in SEQ ID No. 2, the nucleotide sequence of the probe is shown in SEQ ID No. 3, and the like scheme. The present application can detect and identify bovine epidemic fever virus, bovine herpes virus 4, bovine rotavirus and clostridium welchii through four groups of primer probes. The primer probe combination provided by the present application can identify and diagnose four common pathogens of cattle through a one-tube reaction system by fluorescence quantitative PCR method, and can also identify mixed infection samples, has the advantages of accurate identification, high sensitivity, simple operation, short time consumption and the like, and can provide effective technical means for the diagnosis of cattle infectious disease pathogens and disease prevention in China.

The invention discloses a primer probe composition for detecting H9 subtype AIV, NDV and IBV, a kit and application, and belongs to the technical field of fluorescence detection. The sequence of the primer probe combination is shown as SEQ ID NO.1-SEQ ID NO.9, the primer probe combination comprises a primer pair and a probe for detecting the H9 subtype avian influenza virus, the Newcastle disease virus and the infectious bronchitis virus, can detect and distinguish epidemic H9 viruses of different branches, NDV of different genotypes and IBV of different genotypes, does not have cross reaction with other common viruses, and can be used for detecting and distinguishing the H9 viruses of different branches, the NDV of different genotypes and the IBV of different genotypes. The detection sensitivity is high, and the minimum detectable quantity of the three viruses is 0.8 copy, so that the specific, sensitive and rapid detection of H9, NDV and IBV can be realized, and the defects of the existing traditional detection technology are overcome. The method has important practical significance on timely blocking epidemic situation propagation, reducing breeding loss, guaranteeing public health safety and the like.

The invention discloses a monoclonal antibody 3B3 capable of being specifically combined with a group A porcine rotavirus and application, the monoclonal antibody 3B3 is a murine monoclonal antibody, the heavy chain type of the monoclonal antibody 3B3 is IgG1, and the light chain type of the monoclonal antibody 3B3 is kappa; the monoclonal antibody 3B3 for resisting the group A porcine rotavirus is obtained by taking recombinant VP6 protein expressed by a CHO system as an immunogen to immunize a Balb / c mouse and screening through a hybridoma technology, has very excellent biological performance, not only has high affinity to rotavirus VP6 protein, but also has good reactivity to the group A porcine rotavirus. The sensitivity of the kit can reach 1: 256, and the kit has no cross reaction with positive serum of antibodies of six common diseases in pig farms. The method is relatively good in repeatability, and the coincidence rate with an indirect immunofluorescence detection result reaches 95.65%.

The invention discloses an intelligent medical inquiry auxiliary diagnosis system based on symptom reasoning and a knowledge graph. The intelligent medical inquiry auxiliary diagnosis system comprises a knowledge graph construction module, a common disease diagnosis module, a multi-round dialogue inquiry module, a multi-modal data fusion module, a treatment scheme generation module and a follow-up visit management module. The data layer is responsible for medical knowledge storage and case data management, the logic layer is used for realizing a reasoning algorithm and a dialogue strategy, and the application layer is used for providing a user interaction interface and an external system interface. Systematized collection of symptoms is achieved by constructing a large-scale medical knowledge graph and designing a multi-round dialogue inquiry strategy, the disease posterior probability is calculated by applying a Bayesian probabilistic reasoning model, multi-modal diagnosis is conducted by fusing medical images and inspection data, a personalized treatment scheme is generated, and medication safety is checked. And intelligent inquiry, accurate diagnosis, reasonable medication and whole-course management of the patient are realized.

Bacterial infection detection is often limited by the sensitivity of a traditional immunoassay, and is derived from signal load-recognition efficiency tradeoff caused by carrier diffusion limitation and steric hindrance. Therefore, an M13 bacteriophage mediated'biological lever 'system is constructed, recognition and signal amplification are separated in space, pIII protein displays a nano antibody to realize target specific capture, pVIII protein is covalently connected with a high-density DNA probe to serve as a signal interface, low-leakage catalytic hairpin assembly (CHA)-CRISPR / Cas13a cascade amplification (LUCAS) is triggered, and a single recognition event is converted into multi-stage signal enhancement. The design effectively improves transduction efficiency and inhibits background interference. Acinetobacter baumannii is taken as a model, and the system realizes the detection limit of 10 CFU / mL within 2 hours, is stable in labeled bronchoalveolar lavage fluid and has no cross reaction to common pathogens. The strategy provides powerful support for sensitive and rapid detection of pathogens.

The present application relates to a kind of micro-droplet digital PCR detection kit and application for detecting central nervous system infection multiple viruses, belong to the field of reagent kit technology.The specific primers and detection probes of eight viruses EV, HPeV, HSV1, HSV2, VZV, CMV, HHV6 and EBV are designed and synthesized, and the detection method is optimized.The minimum detection limit of the method for EV, HPeV, HSV1, HSV2 is 5, 10, 5 and 10 copies per reaction respectively, and the minimum quantitative limit is 10, 10, 50 and 10 copies per reaction respectively.The minimum detection limit of VZV, CMV, HHV6 and EBV is 5 copies per reaction, and the minimum quantitative limit is 50 copies per reaction.The two groups are linear in the range of 2-2000 copies per reaction;14 other common pathogens of CNSI are detected, and no cross reaction is found, and the specificity is good.

This invention discloses a qPCR primer-probe combination, kit, and method for specifically detecting 16SrII group areca etiolated phytoplasma, belonging to the interdisciplinary field of molecular biology and plant pathology. The primer-probe combination includes a forward primer qTuf-F4-2 (SEQ ID NO:1), a reverse primer qTuf-R4 (SEQ ID NO:2), and a fluorescent probe qTuf-P4-1 (SEQ ID NO:3), targeting 16SrII group phytoplasma. tuf This gene conserved region demonstrates high specificity for 16SrII group areca yellowing phytoplasma in qPCR reactions, exhibiting no cross-reactivity with 16SrI group, 16SrXXXII group phytoplasma, areca genome, common pathogens, or endophytes. The limit of detection is 14.35 copies / μL, and the sensitivity meets the requirements for detecting low-load samples. The coefficient of variation for intra- and inter-group repeatability is less than 1%, indicating good stability. The kit and detection method developed based on this combination are simple to operate, highly sensitive, and provide stable results, making them suitable for the accurate diagnosis, pathogen typing, and field epidemic monitoring of areca yellowing disease, providing key technical support for the scientific control of 16SrII group phytoplasma.

The invention belongs to the technical field of gene detection, and particularly relates to a primer probe composition for detecting H9 subtype AIV, NDV virulent strains and IBV, a kit and application. The primer probe composition for detecting the H9 subtype AIV, the NDV virulent strain and the IBV comprises an upstream primer, a downstream primer and a probe which are designed aiming at the H9 subtype AIV virulent strain, and the sequences of the upstream primer, the downstream primer and the probe are shown as SEQ ID NO.1-3; the sequences of the upstream primer, the downstream primer and the probe are as shown in SEQ ID NO.4-6, and the probe is designed for the H9 subtype NDV virulent strain; the sequences of the upstream primer, the downstream primer and the probe designed aiming at the IBV are as shown in SEQ ID NO.7-9; according to the invention, H9 subtype AIV, NDV virulent strains and IBV with different branches or genotypes can be specifically detected, cross reaction with other common viruses is avoided, and the detection sensitivity is high.

The present application relates to the field of functional microorganism screening and application technology, and particularly relates to a Bacillus subtilis and application thereof in disease control of Pyrus ussuriensis. The Bacillus subtilis is Bacillus subtilis SNBS-3, which has been preserved in the China General Microbiological Culture Collection Center (CGMCC) on June 9, 2022, and the address of the CGMCC is No. 1, Yihuangyijin 3hao, Beichenxilu, Chaoyang District, Beijing, and the Institute of Microbiology, Chinese Academy of Sciences, and the preservation number is CGMCC No. 25040. The Bacillus subtilis can produce protease and chitinase in a high yield, and has a good control effect on common diseases of Pyrus ussuriensis such as black spot and brown spot, and has a wide application prospect.

The invention belongs to the technical field of medical data processing, and particularly relates to an algorithm library matching system and a difference calculation method for a complex chronic common disease scene, and the system comprises a hierarchical algorithm library construction module which is used for constructing a common disease algorithm library and realizing the structured storage of an algorithm module; meanwhile, relying on a real-time updating interface, newest clinical evidence and diagnosis and treatment practice data are accessed, and algorithm rules of all levels are dynamically optimized; and the multi-dimensional matching module is used for completing algorithm matching by utilizing three-stage matching and ensuring that a matching result covers disease generality, interaction characteristics and individual differences. According to the method, the hierarchical algorithm library of the three-level architecture of the base layer, the interaction layer and the individual layer is constructed, and the multi-dimensional matching module is combined with the extraction of the disease interaction characteristics and the accurate matching of the interaction rule module, so that the interaction relationship among multiple diseases is effectively incorporated, and the diagnosis and treatment scheme conflict caused by the matching of a single disease algorithm is avoided.

The application discloses a kind of target sequencing primer set and kit for detecting multiple rodent carrying pathogenic microorganisms.The nucleotide sequence of the primer set is shown as SEQ ID NO:1-400.The kit comprises the primer set shown as SEQ ID NO:1-400.The application can simultaneously detect 50 kinds of rodent carrying pathogenic microorganisms, with the advantages of high detection throughput, clear pathogen spectrum range, low sequencing cost, high sensitivity, and no influence of host nucleic acid, etc.The target sequencing primer set and kit are developed for common pathogen spectrum carried by rodents, which can effectively make up for the shortcomings of prior art, expand the detection range, improve the detection sensitivity, and provide key technical support for early warning, prevention and control of plague, hemorrhagic fever with renal syndrome, fever with thrombocytopenia syndrome and other rodent-borne diseases.

The invention belongs to the technical field of molecular detection, and particularly relates to a respiratory tract pathogen detection kit based on capillary electrophoresis and application of the respiratory tract pathogen detection kit, the kit can detect common pathogens which are possibly infected by respiratory tract patients and cannot be detected and determined through a conventional technology or a kit, and the kit at least has the technical advantages of being high in sensitivity and specificity.

The invention belongs to the technical field of molecular biology, and particularly discloses a joint pathogen detection method based on a micro-fluidic chip technology and application of the joint pathogen detection method. The detection method is based on parallel control of multiple reaction cavities of a micro-fluidic chip, and specific primers and probe mediated PCR amplification and signal detection are combined; 20 pathogens, namely 13 common pathogens and 7 high-risk pathogens, of respiratory infectious diseases can be jointly detected. Compared with a conventional method, the method has the advantages that respiratory infectious diseases and high-risk pathogens can be synchronously screened, pathogen subtypes can be accurately recognized, and the method is adaptive to multi-scene rapid detection so as to fill the blank of a combined screening technology; meanwhile, the detection cost is reduced, the flux is improved, primary popularization is easy, clinical misdiagnosis and missed diagnosis are reduced, and support is provided for public health prevention and control.