Primer probe group, detection reagent and detection kit for detecting glycogen synthase kinase 3 gene
By providing a primer probe set and detection kit for specific detection of the GSK-3β gene, combined with the fluorescent quantitative PCR method, the problem that the existing technology cannot specifically detect the expression of the GSK-3β gene related to diabetic kidney disease is solved, and a highly specific and sensitive detection effect is achieved, providing a reliable detection method for the early diagnosis of diabetic kidney disease.
Patent Information
- Application Number
- CN202510835589.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-06-20
- Publication Date
- 2025-09-05
AI Technical Summary
The existing technology lacks reagents that can specifically detect the expression levels of the GSK-3β gene related to diabetic kidney disease, and the GSK-3β target is mainly used in the field of non-diabetic kidney disease, which cannot provide effective early diagnosis and treatment guidance.
Provided are primer probe sets and detection kits for specific detection of the GSK-3β gene, including primer sets and probes. Through the fluorescent quantitative PCR method, the conserved sequence of the GSK-3β gene is used as a detection marker, combined with an internal standard gene, to achieve accurate detection of patients with diabetic kidney disease.
It achieves high specificity, high sensitivity and high accuracy in detecting patients with diabetic kidney disease, can quickly identify the expression level of GSK-3β in diseased tissue or urine, provides a reliable basis for diagnosis, and avoids interference from non-specific amplification and other pathogens.