The invention provides a neural fingerprint extraction and classification method. The method comprises an image acquisition step, a brain structure annotation step and a neural fingerprint extractionstep in sequence. The method has the advantages that information mining is performed on magnetic resonance images in different sequences, a unified fingerprint extraction and establishment method forall brain areas is formed through unified structure annotation establishment and positioning, a comprehensive neural fingerprint system is constructed, neural fingerprint standard libraries for different sexes and different ages are established, and therefore brain health assessment, disease classification and parting and disease prediction can be performed on a to-be-tested target; through the establishment of the neural fingerprint standard libraries, quantitative assessment and classification can be performed on brain development, brain senescence, neural diseases and mental diseases of a to-be-tested object, and talent assessment, advantage assessment, senescence assessment, rehabilitation assessment, assistant diagnosis, disease prediction and other technologies can be formed; and a standard technical foundation is laid for advantage cultivation, brain healthcare and diagnosis and prevention of brain diseases of human talents.