43 results about "Complementation" patented technology

The invention provides a spatial omics data completion method and system based on a variational graph auto-encoder, and relates to the technical field of bioinformatics. Extracting partial common genes in the data pair to obtain a feature matrix; based on the spatial position information, obtaining a first sub-adjacency matrix of cells in the spatial transcriptomics sequencing data; respectively obtaining a second sub-adjacency matrix of the cells in the single-cell RNA sequencing data and a third sub-adjacency matrix of the cells in the data pair based on gene expression similarity; combining the first sub-adjacency matrix, the second sub-adjacency matrix and the third sub-adjacency matrix to obtain a final adjacency matrix; and inputting the feature matrix and the final adjacent matrix into a pre-trained variational graph auto-encoder network to complete the deletion gene of the space transcriptomics. According to the method, the position information and gene expression characteristics between idle data cells can be effectively utilized, errors can be reduced, and meanwhile, the similarity between complementation genes and true values can be improved.

The invention provides a deletion strain and a supplementation strain of related genes of Aeromonas dakara PhoBR and QSS as well as a construction method and application of the deletion strain and the supplementation strain, a deltaahyR, deltaluxS and deltaqseB gene deletion strain and a supplementation strain as well as a deltaphoB deltaahyR, deltaphoB deltaluxS and deltaphoB deltaqseB double-gene deletion strain are successfully constructed, it is proved that the PhoBR can directly regulate and control the related genes ahyR, luxS and qseB of the QSS in the Aeromonas dakara, negative regulation and control on the ahyR and the luxS and positive regulation and control on the qseB are shown, the QSS related genes can be directly regulated and controlled in the Aeromonas The regulation and control are crucial to the survival and environment adaptation of bacteria. Besides, through a related bioinformatics method, possible binding domains of part of PhoB and three QSS related genes ahyR, luxS and qseB have been predicted, and a scientific basis is further provided for immune prevention and treatment of Aeromonas dakara.

The present application relates to the field of gene editing technology, bioengineering and application of microbial fermentation, and discloses a Pichia pastoris high-efficiency gene editing system, a construction method thereof and application in a high-yield cordycepin engineering strain.The construction of the Pichia pastoris CRISPR-Cas9 gene editing system mainly includes the following steps: the histidine HIS4 nutritional deficiency back complementation is used to realize the resistance-free assembly of KhCas9; the sgRNA-tRNA array and the IIS type restriction endonuclease combination are used to simplify the gene editing operation and realize the simultaneous and high-efficiency editing of multiple genes; and the Brex27 domain is fused with KhCas9 to strengthen the homologous recombination efficiency. By using the gene editing system, through the optimization of methanol assimilation, 3'-AMP and adenosine precursor supply, ATP / NADPH energy supply and the like, the construction of a cordycepin engineering strain without resistance gene residues is realized for the first time, the gene editing efficiency of Pichia pastoris is greatly improved, and a powerful tool is provided for the industrialized production of cordycepin.

The present application relates to the technical field of genetic engineering, and specifically discloses a Chlamydomonas reinhardtii SnRK2.7 gene and application thereof in adaptation to osmotic stress and maintenance of cell survival. The present application first discloses SnRK2.7 the key biological function of the gene in maintaining Chlamydomonas cell homeostasis. By knocking out the gene through CRISPR / Cas9 gene editing technology and adopting an expression frame driven by an endogenous promoter for genetic back complementation, it is confirmed that SnRK2.7 the gene positively regulates cell osmotic stress tolerance and maintains cell long-term survival. In addition, the present application first discovers that the SnRK2.7 protein is specifically located in the Chlamydomonas expansion vesicle, which provides direct spatial evidence for the cell function. The above discovery not only expands the understanding of the osmotic stress signal transduction mechanism of Chlamydomonas, but also provides a new gene target and research tool for the improvement of microalgae stress adaptability, and has important basic research value and biological technology application potential.

The invention discloses a rice disease resistance defense regulation gene SRWD2 as well as an encoding protein and application thereof. According to the invention, a rice disease spot-like mutant tb18 is taken as an experimental material, a rice disease resistance defense regulation gene SRWD2 is separated through strategies such as MutMap positioning and transgene complementation, the nucleotide sequence of the gene is shown as SEQ ID NO.1, and the sequence of a protein coded by the gene is shown as SEQ ID NO.2. The biological function of the SRWD2 gene is analyzed, a theoretical basis is provided for clarification of a molecular mechanism of programmed cell death and defense reaction of plants, the gene mutation site is introduced into the plants through gene engineering or conventional means, the disease resistance can be remarkably enhanced, and the gene has important application value in the aspect of disease-resistant variety cultivation of the plants.

The invention belongs to the technical field of tobacco gene engineering, and particularly relates to a CCD4 protein mutant G312S and application thereof. Compared with a wild type CCD4 protein, the mutant has the following mutation: p. G312S, and the NCBI (National Center of Biotechnology Information) accession number of the wild type CCD4 protein is AKO22632.1. According to the invention, the substrate beta-carotene is docked to a CCD4 enzyme activity pocket, a saturated mutant library is constructed, screening is carried out through a bacterial color complementation experiment, and after specific site amino acid mutation, degradation of the substrate beta-carotene is accelerated, so that a certain technical foundation is laid for further cultivation of new varieties of tobacco crops.

The invention discloses a trans-complementary system for producing South African foot-and-mouth disease virus-like particles with transcription and replication capabilities and application of the trans-complementary system. The trans-complementary system comprises a cell line stably transfected with an SAT2FMDV P1 gene and an FMDV-mCherry replicon plasmid, and can be used for producing South African type 2 foot and mouth disease virus-like particles (SAT2FMDV trVLPs) with transcription and replication capabilities. Due to the defect of internal nucleic acid of SAT2FMDV trVLPs, only single-round infectivity exists, safety is high, and biosafety degradation can be achieved. Therefore, the invention also utilizes SAT2FMDV trVLPs to simulate natural virus to establish a serum trace neutralization test system, and replaces the traditional evaluation test of the neutralizing antibody of foot-and-mouth disease positive serum or vaccine immune serum in clinic, so that the SAT2FMDV serum neutralization test can be safely and efficiently completed under the condition of a biosafety secondary laboratory. The invention provides an efficient and safe virology tool, and has huge potential and practical value in the aspects of reducing biological safety risks and simulating SAT2FMDV live virus research.

The invention relates to the technical field of nucleic acid detection, and discloses a method and a device for detecting and distinguishing ginseng and American ginseng and terminal equipment, and the method comprises the following steps: enriching specific DNA fragments of ginseng and American ginseng by using a multi-enzyme isothermal amplification technology, and screening high-specificity crRNA by combining with a PAM sequence of Cas protein. During detection, a two-channel detection system is constructed by using crRNA, Cas protein and a test probe; after the crRNA accurately recognizes a target sequence in a to-be-tested sample according to a base complementation principle, the trans-cleavage function of the Cas protein is activated, and the test probe is cleaved to form a visual signal. Through two-channel logic, not only can single species be identified, but also a mixed adulteration sample can be identified, the method has the advantages of high sensitivity, strong specificity, constant temperature in the whole process, no need of a precise variable temperature instrument and the like, the problem of interference of near-source species is effectively solved, and simple, convenient and rapid detection of ginseng and American ginseng is realized.

The invention belongs to the field of biological detection, and particularly relates to a specific visual detection reagent for influenza A virus drug resistance point mutation and a detection method thereof. The reagents comprise a primer pair X, a connecting probe pair Y and a detection probe pair Z; the primer pair X is a primer pair which does not participate in the recognition complementation of a detection target and is used in a subsequent RPA (recombinase polymerase amplification) reaction; the connection probe pair Y is composed of a probe SP (Specific Probe) and a probe CP (Common Probe), the detection probe pair Z is composed of a detection probe WT-DP (Wildtype Determination Probe) and a detection probe MT-DP (Maximum Determination Probe), and the connection probe pair Y and the detection probe pair Z are single-stranded DNA molecules composed of 46-52 nucleotides. According to the present invention, the ligation reaction and the RPA probe method are combined, such that the SNP site detection under the normal temperature condition can be achieved, the precise thermal cycle instrument is not required, and the turnover time is short; the background signal is very low, and the sensitivity and the specificity are high. As the design process of the probe is simple and universal, the probe has a wide application prospect in the aspect of diagnosing new infectious diseases and new variants.

The invention relates to the technical field of plant breeding, discloses a high-quality and high-yield alfalfa directional breeding method and application thereof, and aims to solve the problem that western alfalfa breeding lacks an integrated molecular marker screening system which is combined with a time sequence stress phenotype and can be simultaneously associated with high-protein quality, drought resistance and saline-alkaline characteristics. The method comprises the following steps: constructing association and verification double groups, and performing stress memory activation, ploidy and genetic purity verification and quality preliminary screening; simulating three-stage time sequence stress from a seedling stage to an early overwintering stage, and measuring four-dimensional phenotypic indexes of yield, quality, stress response and habitat adaptation; gWAS analysis is carried out, a double-character co-association marker is mined, and molecular expression, genetic complementation and multi-environment verification are carried out; finally, new germplasm is created through phenotype primary screening, genotype verification, ecological adaptation three-level screening and field trials, the breeding efficiency is improved, the bred variety can stably adapt to the western composite habitat, the high-yield and high-protein characteristics and the soil improvement capacity are both considered, and the field ineffective workload is greatly reduced.

The invention discloses application of a millet plant type regulation gene. Plant types comprise plant height and / or tillering. The SiDT1 gene (the nucleotide sequence is shown as Seq ID No: 1) is cloned by utilizing a dwarf tillering millet mutant through a map-based cloning method, the function of the gene is verified through transgene complementation, the gene encodes a gibberellin 3 oxidase (the amino acid sequence is shown as Seq ID No: 2), and the characters of plant height, tillering and the like are influenced by influencing synthesis of gibberellin active substances. The invention also discloses a mutant gene of the gene, a protein coded by the mutant gene, a plasmid containing the mutant gene, a plant expression vector, a host cell and application of the mutant gene in regulation and control of plant types. The invention also discloses an application of a mutant containing the mutant gene in regulation and control of the millet plant type, and discloses an application of a molecular marker closely linked with the millet plant type regulation and control gene. The invention provides a new thought for shaping a new variety of ideal plant types of plants.

The invention discloses an efficient and accurate wheat parent matching method and system based on the distance between a core germplasm and a genome, and belongs to the technical field of cross breeding. The matching method comprises the following steps: acquiring whole genome SNP genotype data of a breeding population, constructing a core germplasm of which the sample size is smaller than that of an original population but the original genetic diversity is greater than or equal to 95%, analyzing the population genetic structure of the core germplasm, calculating the genome distance between individuals of the core germplasm, and selecting the whole genome SNP genotype data of the breeding population. And screening Top N combinations with maximum and minimum genome distances by taking parents from different genetic subgroups as a primary condition and taking an interval from the top quartile to the maximum value of the genome distance as a primary screening range, and outputting a recommended hybrid combination list. According to the method, quantitative indexes of the genetic population structure and the genome distance are innovatively coupled through an algorithm model, double screening of subpopulation complementation and distance optimization is achieved, decision logic is more scientific, and prediction accuracy is higher.

The present invention relates to a rice SDT6 gene, a protein encoded therein and an application thereof. The present invention provides an SDT6 gene for regulating rice plant type, and the nucleotide sequence of the SDT6 gene is shown in SEQ ID NO.2 or SEQ ID NO.3. The amino acid sequence of the protein encoded by the SDT6 gene is shown in SEQ ID NO.1. The present invention screened a mutant with semi-dwarf stem and increased tillering and successfully cloned the target gene for regulating the phenotype. After the gene was knocked out in the japonica rice ZH11 material by CRISPR / Cas9 technology, the rice plant height was significantly reduced and the tillering was significantly increased. The gene function was further confirmed by gene function complementation verification, and the gene SDT6 was preliminarily verified to regulate the plant height and tillering of rice, and it was found that it was related to the auxin regulation mechanism in rice. The present application is of great significance in breeding rice, indica or japonica rice with excellent yield and / or lodging resistance.

The invention provides a system and a method for complement expression after migratory locust gene knockout, and belongs to the technical field of insect gene editing, the system for complement expression after migratory locust gene knockout comprises an overexpression plasmid and a living transfection reagent; the overexpression plasmid carries a GAPDH (glyceraldehyde-phosphate dehydrogenase) promoter and a complement gene; the method comprises the following steps: 1) cloning a back-filling gene to a carrier skeleton carrying a GAPDH promoter; 2) respectively dissolving the overexpression plasmid and the living transfection reagent, and then mixing and incubating to obtain a system for complement expression after migratory locust gene knockout; 3, the system is delivered to a migratory locust living body, by means of the system for complement expression after migratory locust gene knockout, the expression quantity of the gene in a migratory locust gene knockout mutant can be specifically complemented, complement expression of the target gene can be detected at the mRNA and protein level, and an effective means is provided for accurately verifying the function of the knockout gene.

The application discloses application of protein FoUPE7 in reducing pathogenicity of banana fusarium wilt. The application constructs a knockout plasmid and a back complementation plasmid of a gene (FoUPE7) coding protein FoUPE7 of banana fusarium wilt, transforms corresponding protoplasts by using the knockout plasmid and the back complementation plasmid respectively, and selects FoUPE7 knockout mutants and back complementation mutants to find and analyze pathogenicity. It is found that knockout of the gene FoUPE7 can significantly reduce the pathogenicity of banana fusarium wilt, and the pathogenicity of banana fusarium wilt is restored and equivalent to that of the wild type after back complementation of the gene, which indicates that the protein FoUPE7 is a pathogenicity-related protein of banana fusarium wilt, regulates the pathogenicity of banana fusarium wilt, and can be used as a target for prevention and treatment of banana fusarium wilt. The application provides a new target for prevention and treatment of banana fusarium wilt, and is beneficial to development of a banana fusarium wilt prevention and treatment preparation.

The present invention discloses the application of protein MoUPE3 in regulating the pathogenicity of Magnaporthe oryzae. In the present invention, the Magnaporthe oryzae knockout mutant ΔMoUPE3 was obtained by knocking out the gene encoding protein MoUPE3 in Magnaporthe oryzae, and the complemented mutant ΔMoUPE3-com was constructed by complementation. By detecting the colony morphology, sporulation amount, stress resistance, pathogenicity, etc. of the wild type of Magnaporthe oryzae, the Magnaporthe oryzae knockout mutant ΔMoUPE3, and the complemented mutant ΔMoUPE3-com, it was found that the gene encoding protein MoUPE3 of Magnaporthe oryzae can regulate the sporulation amount, stress resistance, and pathogenicity of Magnaporthe oryzae, and can be used as a target for controlling rice blast. The present invention enriches the pathogenicity-related genes of Magnaporthe oryzae, and at the same time provides target genes for the development of effective fungicides, which is helpful for the control of rice blast.

The application belongs to the field of plant breeding and biotechnology, and particularly relates to how to reduce the gelatinization temperature of rice by gene OsGLIP11 and application of the gene in creating new germplasm of rice with low gelatinization temperature. The application discloses a nucleic acid sequence of a coding region of gene OsGLIP11, a protein sequence coded by the gene, a target site nucleic acid sequence of CRISPR-Cas9 knockout, a nucleic acid sequence of a knockout mutant and a protein sequence of the knockout mutant, as shown in a standard nucleotide or amino acid sequence table of ST.26; after the gene OsGLIP11 is knocked out by using CRISPR-Cas9 technology, it is found that the gelatinization temperature of rice of the transgenic plant with the gene OsGLIP11 knocked out is significantly lower than that of a wild type variety; after the transgenic plant with the gene OsGLIP11 knocked out is subjected to genetic function complementation by using transgenic function complementation technology, the gelatinization temperature of rice of the function complementation transgenic plant has no significant difference with that of the wild type variety. The application can create new germplasm of rice with low gelatinization temperature by using the gene OsGLIP11.

The invention discloses a method for preventing and controlling maize chlorotic mottle virus by using an anti-disease gene ZmCDPK7 and application of the anti-disease gene ZmCDPK7, and belongs to the technical field of plant protection. According to the application, through instantaneous silencing of the ZmCDPK7, an overexpression and knockout line of the ZmCDPK7 is constructed, the resistance reaction of the overexpression and knockout line to maize chlorotic mottle virus infection is analyzed, and it is proved that the ZmCDPK7 positively regulates the immunity of maize to the MCMV. In order to further clarify the molecular mechanism of the ZmCDPK7 for regulating and controlling immunity, interaction between the ZmCDPK7 and a key pathogenic factor P31 of the MCMV is discovered and proved through technologies such as yeast two-hybridization and bimolecular fluorescence complementation. ROS detection proves that the ROS content of the ZmCDPK7 knockout strain is higher than that of a wild type after MCMV infection. In biological and abiotic stress reactions, the resistance of the ZmCDPK7 knockout strain to biological and abiotic stress is proved to be reduced.

The invention discloses a rice gene OsLTD1 and application thereof. The CDS sequence of the rice gene OsLTD1 is as shown in SEQ ID NO.1, and the amino acid sequence of encoding protein of the rice gene OsLTD1 is as shown in SEQ ID NO.3. The invention further discloses a preparation method of the rice gene OsLTD1. Through gene localization and function complementation verification technologies, it is identified that function deletion of the OsLTD1 gene can significantly enhance the resistance of rice to rice blast. The function deletion mutant osltd1 of the gene shows leaf yellowing and lesion-like phenotypes from a seedling stage to a tillering stage, and shows broad-spectrum resistance to a plurality of pyricularia grisea physiological races. According to the invention, the broad-spectrum disease-resistant character and the excellent agronomic character are efficiently combined through a visual leaf color marker associated with gene function deletion, a new high-yield and disease-resistant rice variety is finally cultivated, and a new technical scheme and a new gene resource are provided for solving the problem of rice blast prevention and treatment.

The invention discloses a nucleic acid combination for detecting FGFR2 gene fusion mutation in bile duct cancer and a detection method, and develops a design method of a universal joint based on digital PCR (Polymerase Chain Reaction) by utilizing the characteristic that most breakpoints of FGFR2-driven fusion genes are positioned at the tail end of an FGFR217 exon, and all fusion of which the breakpoints are the tail end of the FGFR217 exon can be detected at one time. In addition, a tail sequence Tail is specially designed for a fusion specific primer, and the purpose of inhibiting FGFR2WT cDNA amplification is achieved through complementation of the tail sequence Tail and a sequence, close to a breakpoint, of FGFR2WT exon18, so that the amplification efficiency of FGFR2fusion is greatly improved. The detection method is convenient to operate, and has the characteristics of high sensitivity, high accuracy, low cost and the like.

This invention relates to a rice leaf color regulating gene in the field of rice molecular genetic breeding. OsPRDA1 and its applications, the gene OsPRDA1 The nucleotide sequence is shown in SEQ ID No. 1, and the amino acid sequence of the encoded protein is shown in SEQ ID No. 2. This invention clones the target gene from a rice albino lethal mutant using Mutmap+ technology. OsPRDA1 The function of the gene was verified using CRISPR / Cas9 gene editing technology and complementation experiments. OsPRDA1 The protein encoded by this gene is located in chloroplasts and participates in rice chloroplast development by regulating the expression of chloroplast genes. This invention can be applied to molecular genetic breeding of rice leaf color traits and is of great significance for further understanding the mechanism of rice chloroplast development and improving rice yield.

The invention relates to a morchella monospore hybridization breeding method, which belongs to the technical field of strain breeding, and comprises the following steps: (1) selecting morchella, obtaining a tissue isolation strain through a tissue isolation method, and obtaining a monospore isolation strain through a monospore isolation method; (2) respectively carrying out mating type gene detection on genomes of the tissue isolation strain and the single spore isolation strain; (3) in the same species, respectively selecting single spore isolation strains only carrying MAT1-1-1 mating type genes and single spore isolation strains only carrying MAT1-2-1 mating type genes for pairing hybridization; and (4) selecting the successful single spore hybrid combination and the corresponding two parent strains to perform cultivation test and agronomic character evaluation, and selecting a hybrid combination with yield and disease resistance superior to those of a single parent. The invention provides a high-efficiency breeding scheme capable of being popularized, reveals a remarkable effect of a mating type gene hybrid combination on the aspects of increasing the yield of morchella esculenta and enhancing disease resistance, and verifies positive regulation and control of mating type gene complementation on the yield and stress resistance.

The invention discloses a sclerotinia sclerotiorum gene SsCRZ1 and application thereof in regulation and control of infection pad formation and pathopoiesis, and belongs to the technical field of microbial genetic engineering. The DNA sequence of the gene SsCRZ1 is as shown in SEQ ID NO: 1, and the amino acid sequence of the protein coded by the gene SsCRZ1 is as shown in SEQ ID NO: 2. Gene knockout and refilling experiments prove that the infection pad forming ability of sclerotinia sclerotiorum and the pathogenicity of sclerotinia sclerotiorum to host plants can be remarkably weakened by deletion of the SsCRZ1 gene, and the functions can be recovered by refilling the gene. Therefore, the SsCRZ1 gene is a gene necessary for formation and pathopoiesis of a sclerotinia sclerotiorum infection pad. According to the invention, the function of the SsCRZ1 gene in sclerotinia sclerotiorum is defined for the first time, and a theoretical basis is provided for the gene as a new target for prevention and control of sclerotiniose.

The application discloses a single-stranded DNA molecular weight standard based on light-controlled self-assembly and a preparation method thereof, and comprises the following steps: S1, chemically synthesizing a DNA sequence containing a photosensitive bridging group, which can self-assemble to form a hairpin structure, and self-assembly can also occur between the hairpin structures; S2, annealing the DNA sequence, and self-assembly of individual DNA sequences to form hairpin structures; S3, mixing the hairpin structures with a nucleic acid ligase, under ultraviolet light, the photosensitive bridging group is broken, the exposed single-stranded sequence is paired with other hairpin structures through base complementation, and cascade self-assembly is realized through the nucleic acid ligase; S4, heat inactivation treatment; S5, ultraviolet light treatment is performed again, so that all the photosensitive bridging groups are broken; and S6, an electrophoresis loading buffer is added, and low-temperature preservation is carried out. The application adopts a one-pot method to rapidly prepare the DNA molecular weight standard, has the advantages of simple process, low manufacturing cost and strong expandability, and has a good application prospect.

The present invention discloses the application of the ERS1 gene in regulating the thermosensitive sterility of rice, including obtaining a sterile mutant by gene editing the wild type of medium indica 3037 and naming it ers1-2 (glutamyl-tRNA synthetase), and further verifying the genetic relationship between the ERS1 gene and the sterile phenotype through complementation experiments. The mutant exhibits a typical thermosensitive sterile phenotype, with a fruit set rate of only 20% at high temperature and a fruit set rate of 70% at low temperature. Further cell biological analysis of the mutant found that the pollen development and meiotic process of the mutant were severely delayed at high temperature, leading to male sterility, and these defects can be restored at low temperature, thereby giving the mutant the characteristics of thermosensitive sterility. The mutation of the ERS1 gene has created a new thermosensitive sterile line in rice, which has great potential in rice hybrid breeding.

The invention discloses a rice disease resistance defense regulation gene ECDR1 as well as an encoding protein and application thereof. According to the invention, a rice disease spot-like mutant ecdr1 is taken as an experimental material, a rice disease resistance defense regulation gene ECDR1 is separated through strategies such as map-based cloning and transgene complementation, the nucleotide sequence of the gene is as shown in SEQ ID NO.1, and the sequence of a protein coded by the gene is as shown in SEQ ID NO.2. According to the invention, the biological function of the ECDR1 gene is preliminarily analyzed, and a theoretical basis is provided for clarification of a molecular mechanism of plant programmed cell death and defensive reaction; particularly, the gene mutation site is introduced into a plant through gene engineering or a conventional means, the disease resistance can be remarkably enhanced, and the gene mutation site has important application value in the aspect of cultivation of disease-resistant varieties of the plant.

The invention relates to the technical field of recommendation methods, in particular to a multi-target recommendation method based on auxiliary evolution, which comprises the following steps of: firstly, calculating a comprehensive score of each individual, sorting the individuals, and storing the individuals in a candidate set; selecting individuals from the candidate set to form a main parent population P and auxiliary parent populations P'and P '', and performing crossover operation and mutation operation on the parent populations P, P 'and P' 'to form populations P2, P' 2 and P '' 2; elite strategy is carried out on the populations P2, P '2 and P' '2, and redundant populations are eliminated; according to a sorting result and a calculation result, selecting a corresponding number of individuals and storing the individuals in the parent populations P3, P '3 and P' '3; using a parent population P3; the individuals in the parent populations P, P 'and P' 'replace the individuals in the parent populations P, P 'and P' ', when the three parent populations evolve a certain algebra T1, knowledge migration is carried out among the three parent populations to form advantage complementation, when the three parent populations evolve a certain algebra T2, migration learning is carried out among the users to reduce the situation of falling into local optimum, loop iteration processing is carried out according to the content, and the probability of falling into local optimum is reduced. And after an iteration stopping condition is met, stopping iteration, and outputting a recommendation result. By screening the data, the data processing precision can be effectively improved, and recommendation of two targets of accuracy and diversity is realized.

The present application belongs to the field of plant molecular biology and genetic engineering technology, and particularly relates to a lily anthocyanin synthesis regulation gene LvWRKY75 and application thereof, and the present application has obtained an overexpression vector and a silencing expression vector of the gene. The overexpression LvWRKY75 gene is transformed into wild-type Arabidopsis, and after obtaining the third generation of transgenic plants, the expression amount of the LvWRKY75 gene and downstream target genes in the transgenic lines is detected, and it is proved that the LvWRKY75 gene has the effect of promoting anthocyanin synthesis. The yeast one-hybrid technology, gel retardation experiment and double-molecule fluorescence complementation experiment prove that the LvWRKY75 gene plays a positive regulation role in the regulation of Westin red lily color, and these findings provide important experimental and theoretical basis for further understanding the molecular mechanism and genetic regulation network of plant flower color formation.

The invention discloses an AlGaN / GaN HEMT biosensor for CTC detection and a preparation method of the AlGaN / GaN HEMT biosensor. The biosensor comprises an AlGaN / GaN HEMT device and a TDN-polypeptide probe attached to the surface of the device. The TDN-polypeptide probe is formed by bonding TDN with a polypeptide probe through a base pair; the method comprises the following steps: self-assembling four single-stranded DNA sequences by adopting a DNA origami technology to form TDN, connecting the top end of the TDN with a polypeptide probe through base pair complementation, and fixing the tail end of the TDN on the surface of a chemically modified AlGaN / GaN HEMT device through sulfydryl to realize interface construction of the AlGaN / GaN HEMT device and the TDN-polypeptide probe so as to prepare the AlGaN / GaN HEMT biosensor for CTC detection. The biosensor has the characteristics of rapidness, no mark, high sensitivity, high specificity, simplicity and convenience in operation and low cost, and can be specifically combined with EGFR on the surface of a CTC cell membrane to realize high-specificity CTC detection.

The invention discloses an efficient breeding method for high-quality millet, which aims at solving the problems of traditional millet breeding and comprises the steps of parent selection and matching, planting layout, spike cutting and size thinning, bagging and pollination, generation selection and the like. The parent selection follows the principle of matching and complementation of recessive and dominant characters, a planting mode of'double male parents and female parents' is adopted, and the sowing time is adjusted. Middle grain sizes are reserved by cutting ears and thinning, and flapping pollination is performed at a specific time period after bagging. And performing rice color quantitative selection by combining generation selection with phenotype observation and ABL value measurement by a colorimeter. The multi-generation selection strategy covers from the F1 generation to the F5 generation, and all the generations are screened according to character characteristics. According to practice, the pollination success rate of the method is larger than or equal to 80%, the rice color quantitative selection accuracy rate exceeds 90%, 22 high-quality varieties such as' Jinzhu K1 'and the like are bred, the popularization benefit reaches 18.24 billion yuan, and the method has the advantages that time and labor are saved, the quality identification cost is low, the breeding period is short and the like, and is suitable for large-scale high-quality millet breeding.