45 results about "Saccharopolyspora erythraea" patented technology

The invention provides a method for cloning a saccharopolyspora erythraea expression plasmid (pBlueV) containing a vitreoscilla hemoglobin gene (vgb), belonging to the technical field of gene engineering. In the method, based on the fact that VHb (hemoglobin) can be combined with oxygen to form an oxygenated state, the VHb intervenes the oxygen related metabolic pathway of cells, thereby changing the original biological metabolism mode of the cells under oxygen-limited conditions, promoting the cell growth and protein synthesis under oxygen-deficient conditions, directly or indirectly affecting the action principles of primary metabolism and secondary metabolism of aerobes, firstly constructing an Escherichia coli expression plasmid pQEV, expressing the VHb with biological activity, and further constructing the saccharopolyspora erythraea expression plasmid (pBlueV). In order to realize high-density culture of cells and high-yield fermentation of metabolic products under oxygen-limited conditions, the invention provides an important pathway for homologous recombination of vgb gene elements and saccharopolyspora erythraea chromosome genomes.

The invention provides a method for increasing output of erythrocin by an SACE_5717 gene of saccharopolyspora erythraea. The method comprises the following steps of deactivating the SACE_5717 gene inthe saccharopolyspora erythraea by a gene engineering path, so as to obtain a saccharopolyspora erythraea strain with high output of erythrocin; applying the saccharopolyspora erythraea engineering strain with high output of erythrocin, and fermenting, so as to produce the erythrocin, wherein a nucleotide sequence of the SACE_5717 gene is shown in SEQ ID NO.1; an amino acid sequence coded by the nucleotide sequence is shown in SEQ ID NO.2. The method has the advantages that the SACE_5717 gene in the saccharopolyspora erythraea is deactivated by the engine engineering path, so as to obtain thestrain with high output of erythrocin; the obtained strain with high output of erythrocin is used for fermenting to produce the erythrocin, so that the output of the erythrocin is increased, and the new technical support is provided for the increasing of output of the erythrocin in industrial production.

The invention discloses a method for improving the yield of erythromycin by modifying a saccharopolyspora erythraea SACE_4682 gene. In the saccharopolyspora erythraea, a TetR family transcription regulation gene SACE_4682 is deleted by a genetic engineering method to obtain an erythromycin high-yield engineering strain, and the obtained strain is fermented to produce the erythromycin, so that a new technical support is provided for improving the yield of the erythromycin in industrial production.

The invention belongs to the field of biological fermentation and production and provides a method for producing erythrocin by virtue of fermentation. By taking saccharopolyspora erythraea as a starting germ, the method mainly comprises four steps of preparation of slant strains, preparation of shake-flask seed liquid, culture of seed liquid and process control of a fermentation tank. According to the method, soybean oil is replaced by refined cottonseed oil in a seed tank culture medium, a fermentation tank culture medium and the material supplementing process of a fermentation process, so that the fermentation cost is lowered; the fermentation valence and the content of erythrocin A are greatly increased based on an optimized fermentation process. The method is suitable for large-scale industrial production.

The invention relates to a detection method and a prevention method of phages specific to saccharopolyspora erythraea. The detection method comprises the following steps: mixing cultured saccharopolyspora erythraea mycelia into an upper culture medium of a double-layer plate culture medium, and then paving the cultured saccharopolyspora erythraea mycelia on a solidified lower culture medium of thedouble-layer plate culture medium; placing the culture medium in an environment needing to be detected for detection at regular time and fixed locations after being solidified, taking the culture medium back, carrying out constant-temperature culture for 28-34 hours at 35-37 DEG C and 40-45% RH, and observing and recording results. According to the method disclosed by the invention, the existenceof the phages can be intuitively observed, meanwhile the quantity and the sedimentation rate of the phages can be detected. The detection method of the invention is an effective, rapid, simple and convenient detection method for phages specific to saccharopolyspora erythraea. The detection method of the invention is suitable for detecting ambient air and also suitable for detection in a liquid environment. According to the invention, infection and outbreak of phages specific to saccharopolyspora erythraea can be effectively prevented.

The invention discloses a method for improving erythromycin yield by a saccharopolyspora erythraea SACE_7301 gene pathway. The method is characterized in that gene copy number of the saccharopolyspora erythraea SACE_7301 or gene expression quantity of the SACE_7301 is increased by a gene engineering pathway so as to obtain high-yield engineering strains of saccharopolyspora erythraea erythromycin, and yield of the erythromycin can be improved by fermenting the strains obtained by the technology.

The invention discloses a method of modifying Saccharopolyspora erythromycetes SACE_3980 Genetic method for improving erythromycin production, in Saccharopolyspora erythromycetes, deletion of TetR family transcriptional regulatory genes through genetic engineering SACE_3980 , to obtain high-yield erythromycin engineering strains, and use the obtained strains to ferment and produce erythromycin, which can greatly increase the yield, and provide new technical support for industrial production to increase erythromycin yield.