Method for improving erythromycin yield by saccharopolyspora erythraea SACE_7301 gene pathway

Abstract

The invention discloses a method for improving erythromycin yield by a saccharopolyspora erythraea SACE_7301 gene pathway. The method is characterized in that gene copy number of the saccharopolyspora erythraea SACE_7301 or gene expression quantity of the SACE_7301 is increased by a gene engineering pathway so as to obtain high-yield engineering strains of saccharopolyspora erythraea erythromycin, and yield of the erythromycin can be improved by fermenting the strains obtained by the technology.

Description

technical field [0001] The invention mainly relates to a method for increasing the yield of erythromycin by fermentation, in particular to a method for increasing the yield of erythromycin by increasing the positive regulation gene SACE_7301 on the chromosome of Saccharopolyspora erythromycetes. Background technique [0002] Secondary metabolites of actinomycetes have a wide range of uses, such as antibiotics, anticancer agents, immunomodulators, insect repellents, and insect control agents. Among the 23,000 biologically active secondary metabolites discovered so far, more than 10,000 are produced by actinomycetes. However, the original production of these secondary metabolites is very low, and screening is required to obtain high-yielding strains for industrial production. In the past, industrial production strains were mainly obtained by random physical or chemical mutagenesis. The traditional random mutagenesis technique is not only time-consuming, but also unable to gu...

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Problems solved by technology

In 2007, Oliynyk et al. reported the genome sequence of Saccharomyces NRRL23338, but there are few studies on the regulatory genes of Saccharopolyspora. So far, only bldD (SACE_2077) and SACE_7040 have been reported, and the invention we declared Patent (application number 201210099708.8)

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