The invention discloses healthcare walnut oil with a calcium supplementing function, and relates to the technical field of biological medicine. The healthcare walnut oil is prepared through raw materials as follows: walnut oil, walnut kernels, tea oil, peanut oil, sea clam meats, kelps, goat milk, hyacinth beans, unprocessed rehmannia roots and stearin. A method for specifically preparing the healthcare walnut oil comprises the steps as follows: A, pretreatment of sea clams; B, pretreatment of the kelps; C, pretreatment of the unprocessed rehmannia roots; D, squeezing; E, primary extraction; F, secondary extraction; G, primary mixing; H, secondary mixing; and I, refined filtration. The healthcare walnut oil has the characteristics of complete nourishments, capacity of supplementing calcium and the like; and as edible oil, the healthcare walnut oil is suitable for being eaten by crowds with calcium deficiency, children and old people.