533 results about "Probiotic" patented technology

The invention provides a probiotics plant solid beverage for treating hyperuricemia and gout. The solid beverage is prepared by mixing freeze-dried probiotics powder and plant extract with the addition of proper auxiliary materials. The solid beverage comprises the following components: the plant extract, the auxiliary materials and the probiotics, with the sum of the mass percentage of each component being 100%. The plant extract comprises celery seed powder, sophora flower bud powder and platycodon root powder. The invention has an obvious effect on lowering uric acid and relieving gout.

The invention provides a novel enterococcus strain Enterococcus qinghaiensis SCFF543 and an anti-oxidation application of the novel enterococcus strain Enterococcus qinghaiensis SCFF543, and belongs to the technical field of microorganisms. Wherein the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on June 10, 2025, and the preservation number is CGMCC No.34843. The invention further discloses a preparation method of the strain. The strain is separated from homemade casein of herdsman families in Demaoha City in Haixi, Qinghai province, has the advantages of strong antioxidant capacity, no vancomycin resistance, strong adhesiveness and the like, and can be suitable for preparing probiotic products with antioxidant effects.

The invention provides a cobalt monatomic nano-enzyme system modified by phospholipid polyethylene glycol carboxyl. The cobalt monatomic nano-enzyme system comprises a cobalt monatomic nano-enzyme composite core and phospholipid polyethylene glycol carboxyl compounded on the surface of cobalt monatomic nano-enzyme, the cobalt monatomic nano-enzyme composite core comprises a cobalt monatomic nano-enzyme and ginsenoside loaded on the cobalt monatomic nano-enzyme. The invention also provides a bifidobacterium synergetic cobalt monatomic nano-enzyme system which is formed by combining cobalt monatomic nano-enzyme with ginsenoside Rb1 and further delivering the cobalt monatomic nano-enzyme and ginsenoside Rb1 through bifidobacterium bifidum for targeted therapy of enteritis. The combination of Co SA and Rb1 can enhance the anti-inflammatory efficacy by adjusting a key immune signal pathway, and bifidobacterium bifidum can ensure targeted delivery of microbiota and promote intestinal barrier repair. The synergistic three-component strategy integrating monatomic nano-enzyme antioxidation, natural drug immunoregulation and probiotic targeting is provided, and a promising treatment platform is provided for accurate intervention of IBD.

The invention discloses an ecological planting method for remarkably improving the amino acid content of citrus, which comprises the following steps: (1) mixing and fermenting pruned citrus branches, weed straws, livestock and poultry manure and indigenous probiotic ferment powder to prepare a basic microbial fertilizer, and adding a calcium magnesium phosphate fertilizer and monopotassium phosphate to prepare a special microbial fertilizer for citrus, applying as a base fertilizer in winter; (2) after citrus pruning, in key phenological periods such as a flower bud period, a young fruit period and a fruit swelling period, an indigenous probiotic enzyme diluent with a specific dilution ratio is used for systematic foliage spraying on a tree body. The indigenous probiotic enzyme contains bacillus amyloliquefaciens, saccharomycetes, active enzymes and a plurality of nutrient elements. The method can significantly increase the total amino acid content of citrus fruits, greatly increase the yield, improve the taste of the fruits, enhance the tree body resistance, reduce the use of chemical pesticides, improve soil, and realize quality improvement, yield increase and green sustainable development of citrus planting.

The invention belongs to the technical field of traditional Chinese medicine fermentation, and particularly relates to a traditional Chinese medicine fermentation composition for regulating sleep as well as a preparation method and application thereof. The traditional Chinese medicine fermented composition for regulating sleep is prepared by fermenting spina date seeds, poria cocos, lily, liquorice, polygala tenuifolia, cortex albiziae, lotus seeds and Chinese yams serving as traditional Chinese medicine raw materials through probiotics. The traditional Chinese medicine raw materials with specific components are selected and subjected to gradient fermentation with specific strains, the prepared traditional Chinese medicine composition is remarkable in sleep adjusting effect, and the level of 5-hydroxytryptamine can be remarkably improved.

The invention relates to the technical field of microbial fermentation, and discloses a metabolite fingerprint spectrum construction method of a probiotic fermentation type Chinese herbal medicine preparation. The method is realized by sequentially executing a metabolite data acquisition process, a chromatographic data preprocessing process, a metabolite fingerprint spectrum modeling process, a spectrum construction optimization process and a metabolite fingerprint spectrum generation process. In the metabolite data acquisition process, original metabolite data of the probiotic fermented Chinese herbal medicine preparation is acquired; in the chromatographic data preprocessing process, original metabolite data is subjected to signal processing and feature extraction, and preprocessed chromatographic data is obtained; in the metabolite fingerprint modeling process, a preliminary fingerprint is constructed based on the preprocessed chromatographic data; in the atlas construction optimization process, parameter optimization and accuracy improvement are carried out on the initial fingerprint atlas, and the optimized fingerprint atlas is obtained; in the metabolite fingerprint spectrum generation process, the optimized fingerprint spectrum is integrated, and a final metabolite fingerprint spectrum is generated.

The invention belongs to the technical field of biology, and particularly relates to a synthetic probiotic phenotypic characteristic prediction method and system.The method comprises the steps that a genome is divided into gene units, K-mer sequences containing position weights and codon coding information are extracted from the gene units, and an initial characteristic vector is constructed for each strain; by calculating mutual information between each K-mer feature and a known phenotype, identifying which gene segments are most critical for predicting a target phenotype, and distributing significance weights for the gene segments; after the feature vectors of the known strains are optimized and weighted through the weights, a machine learning prediction model is trained; and when a new synthetic probiotic needs to be predicted, executing the same feature extraction and weighting process on the genome of the new synthetic probiotic, and inputting the obtained vector into the trained model to obtain a prediction result of the phenotypic features of the new synthetic probiotic.

The invention discloses a single-cell probiotic packaging method of thiolated guluronic acid oligosaccharide and a probiotic microcapsule, and relates to the technical field of biological medicines. The packaging method comprises the following steps: mixing a thiolated guluronic acid oligosaccharide solution with a probiotic suspension to form a probiotic-polysaccharide mixed solution; and adding a calcium chloride solution into the probiotic-polysaccharide mixed solution, and stirring to form the single-cell microcapsule encapsulating the probiotics. The packaging method can protect a single strain from being damaged by an external adverse environment to the maximum extent, and the survival rate is increased. Meanwhile, the obtained single-cell microcapsule can improve the digestion of the probiotics, the survival rate of the probiotics under the oxidative stress environment and the antibiotic combination, has high stability, and prolongs the intestinal residence time of the probiotics. An innovative solution is provided for the problems that in the existing medicine field, probiotic preparations cannot improve the gastrointestinal stress resistance of probiotics, and antibiotics lose efficacy when combined.

The present invention discloses a CBP lysine children growth milk powder and a preparation method thereof, and belongs to the technical field of milk powder, the CBP lysine children growth milk powder comprises the following components by mass: a wet process part: 575 parts of whole milk powder, 175 parts of dried skim milk, 175 parts of isomaltooligosacharide (type 50) powder, 30 parts of polydextrose, 28 parts of edible plant blend oil, 4 parts of milk mineral salt, and 13.7 parts of a compound children modified milk powder three-nutrition enhancer; cBP lysine is cooperated with bifidobacterium and seven traditional Chinese medicine extracts, DHA and ARA are accurately proportioned, the calcium absorption efficiency and the intestinal colonization capacity of probiotics are remarkably improved, a three-step mixing method is combined with a two-way shearing low-temperature technology, rapid and uniform mixing is achieved, the activity of the probiotics and the stability of DHA are reserved to the maximum extent, high uniformity of products is ensured, and the product quality is improved. The low-moisture wet-process base powder inhibits moisture absorption of traditional Chinese medicine components, nitrogen protection blocks oxidation in the whole process, the shelf life is prolonged, and the reconstituability is improved.

The present invention generally provides methods and compositions for the treatment of Parkinson's disease and depression and / or anxiety. The invention relates to recombinant microorganisms, particularly gut-colonizing probiotics, modified to produce L-DOPA.

The invention belongs to the field of biotechnology and nutritional medicine, relates to a maternal obesity intervention preparation, and particularly relates to a preparation method of a plant embryo fermentation product and application of the plant embryo fermentation product in blocking of trans-generation delivery metabolic diseases. FWG is prepared through a probiotic fermentation technology, obesity, glucose and lipid metabolism disorder and oxidative stress induced by high-fat diet of a parent body can be remarkably improved, and cross-generation transmission of metabolic diseases is blocked by adjusting composition of intestinal flora of the parent body and offspring and a hypothalamic PI3K-Akt signal channel. The invention reveals that maternal FWG intervention passes through a mechanism that a PI3K-Akt pathway is dominated and multiple pathways are coordinated on the transcriptome level for the first time, the HFD-induced offspring hypothalamus metabolism programming abnormality is reversed, and a new central regulation target is provided for cross-generation metabolic disease prevention. The FWG is a wheat processing byproduct, is free of chemical additives, is suitable for long-term dietary supplement, is a natural and safe nutrition intervention scheme, and is suitable for the field of maternal and infant health.

The invention discloses a compound rumen bypass guanidinoacetic acid additive as well as a preparation method and application thereof, and belongs to the technical field of animal feed additives. The compound rumen bypass guanidinoacetic acid additive provided by the invention is prepared from the following components in parts by mass: 40 to 60 parts of guanidinoacetic acid, 0.1 to 0.5 part of selenium yeast, 5 to 10 parts of nicotinamide, 5 to 12 parts of DL-methionine, 0.01 to 0.05 part of vitamin B12, 1 to 3 parts of compound probiotics, 3 to 5 parts of hydroxypropyl methyl cellulose, 1 to 2 parts of ethyl cellulose, 15 to 25 parts of hydrogenated palm oil and 3 to 5 parts of glyceryl monostearate. The compound probiotics comprise bacillus subtilis and saccharomyces cerevisiae. The compound additive provided by the invention can significantly improve the daily gain of ruminants and reduce the feed-gain ratio, and meanwhile, due to the improvement of the energy utilization efficiency and the enhancement of the antioxidant ability, the immunity of animals is improved, the morbidity is reduced, and the meat quality is improved.

The invention discloses a breeding method for improving pork quality by using Chinese herbal medicine fermentation broth, and relates to the field of livestock and poultry breeding, the method comprises the following steps: preparing composite Chinese herbal medicine fermentation broth, and adding the fermentation broth into daily ration in proportion for feeding in the growth and fattening stage of live pigs, the compound Chinese herbal medicine fermentation liquor is prepared by fermenting a Chinese herbal medicine composition containing astragalus membranaceus, pine needles, eucommia ulmoides and hawthorn through a compound microbial agent; according to the breeding method for improving the pork quality by using the Chinese herbal medicine fermentation liquor, Chinese herbal medicines such as astragalus membranaceus, pine needles, eucommia ulmoides and hawthorn fruits are fermented through a compound microbial agent, the deep synergistic effect of the Chinese herbal medicines and probiotics is achieved, the bioavailability of effective components is remarkably improved, and therefore products better meeting high-end market requirements are produced; meanwhile, probiotics and metabolites in the fermentation liquor can effectively optimize the intestinal health of livestock and reduce the morbidity, and a reliable path is provided for reducing or replacing antibiotics.

The invention discloses a biochar-based microbial soil conditioner and a preparation method thereof, and relates to the technical field of soil improvement. The preparation method comprises the following steps: firstly, carrying out alkali-acid treatment and segmented pyrolysis on raw materials such as wood chips to obtain primary biochar, and then carrying out chemical activation, nano material modification and plasma treatment to obtain the composite modified biochar. Meanwhile, probiotics such as bacillus subtilis and the like are subjected to activation, expanding culture and saline-alkali domestication to obtain directional domestication bacterial liquid, and the directional domestication bacterial liquid is solidified into microspheres by adopting a composite embedding carrier. And finally, mixing the composite modified biochar, the composite embedded microspheres, potassium humate and other auxiliary materials, drying, and sieving to obtain the soil conditioner. The soil conditioner disclosed by the invention can realize an improvement effect on saline-alkali soil and reduce the total salt content; meanwhile, directionally domesticated microorganisms secrete organic acid to neutralize alkaline components, the pH of the soil is adjusted to the range suitable for crop growth, and salt and alkali stress is relieved.

The invention provides a probiotic screening method based on a fine tuning DNABERT model and a convolutional neural network. The method comprises the following steps: acquiring a reference genome sequence and a target sequence sample; preprocessing the reference genome sequence and the target sequence sample; segmenting the preprocessed reference gene sequence, and inputting the segmented reference gene sequence into a DNABERT model for fine tuning training; expanding the number of samples of the preprocessed target sequence, segmenting the sequence by using a sliding window, and inputting the segmented sequence into the fine-tuned DNABERT model for decoding to obtain a representation vector; inputting the representation vector into a CNN model for training, sequencing a target sequence to obtain a to-be-detected sequence sample, encoding the to-be-detected sequence sample, inputting the encoded to-be-detected sequence sample into the CNN model, judging whether the strain is the probiotics or not, and completing screening of the probiotics; according to the method, the data enhancement technology of introducing the reverse complementary sequence and the randomly intercepted sub-sequence is adopted, so that the diversity and the quantity of training data are increased, and the overfitting problem is effectively relieved.

The invention relates to the technical field of bioengineering, in particular to a preparation method of a probiotic and exosome preparation applied to immunoregulation. The probiotics and the umbilical cord stem cell exosome are combined for use, the immune regulation effect is enhanced from multiple targets and multiple ways, the immune function of the body is improved, the exosome carries a specific molecular marker and acts on immune cells in a targeted mode, precise immune regulation is achieved, and the preparation is in an oral form and convenient to use; the exosome is extracted from umbilical cord stem cells, has no pathogenicity and immunogenicity, and is low in adverse reaction risk after being used for a long time, and the prepared combined preparation can be used for treating and preventing various immune-related diseases, and also can be used for daily conditioning of people with low immunity and improving the body resistance; meanwhile, the combined preparation adopts micro-capsule embedding, so that the probiotics can be effectively protected from gastric acid and bile erosion, the survival rate and colonization efficiency of the probiotics in intestinal tracts are improved, the immunoregulation effect is further enhanced, and the combined preparation is convenient to store and transport and has a good application prospect.

Methods of treating a health condition by standardizing patients and donors with pre-treatment protocols to enhance the effects of probiotic administration, improving preservation steps of samples handled, and selecting best matching donors for selection for probiotic administration to effectively treat the health condition.

The invention discloses application of a combination of probiotics GOLDGUT-M520 and GOLDGUT-LR99 in preparation of a product for relieving depression, and belongs to the technical field of biological medicines. The probiotics disclosed by the invention can be used for relieving depressive symptoms, improving related symptoms, assisting in treating depression and preventing depression, and also can be used as a nutritional supplement to help maintain the balance of intestinal microflora and promote intestinal health.

The invention discloses panda-derived tryptophan-producing streptococcus non-lactolyticus C49 and application thereof, and belongs to the field of microorganisms, the strain is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 67209. The strain has good acid-resistant and cholate-resistant characteristics, and can ensure that enough biomass passes through stress of a digestive tract environment; whole genome sequencing finds that C49 is significantly related to the pathway and module of tryptophan biosynthesis. Whole genome sequencing and in-vitro fermentation confirm that C49 has tryptophan biosynthesis capability, and in-vivo animal tests confirm that C49 has synthetic nerve conditioning capability. Therefore, as a probiotic feed additive, the streptococcus non-lactolyticus C49 has a good application prospect in the aspects of improving the feed value, improving the intestinal health of animals, enhancing the immunity of organisms and the like.

The invention provides a decapterus maruadsi deepwater net cage culture method, and belongs to the technical field of decapterus maruadsi culture. According to the method, the submersible net cage is adopted, the decapterus maruadsi is located in the optimal growth water layer at the temperature of 20-25 DEG C all the year round according to rising and falling of seasons (surface water temperature), a guide pipe is adopted for fixed-point feed feeding, and the feed coefficient is reduced to 1.25 or below. According to the method, a water temperature-feeding amount-allicin-probiotics coupling model is established, the enteritis incidence rate in the high-temperature period is controlled within 2%, and annual continuous growth of decapterus maruadsi in deep and far sea is achieved.

The invention discloses a preparation method of a high-stability probiotic solid beverage, and belongs to the technical field of food processing. In order to effectively improve the stability and nutritional value of the probiotic solid beverage, the invention provides the preparation method of the high-stability probiotic solid beverage, and the main process comprises the following steps: (1) strain purification and propagation; (2) freeze-drying the strain; (3) embedding probiotics by a Pickering emulsification method, and carrying out spray drying; (4) blending raw materials of the solid beverage; and (5) packaging and storing. The probiotic solid beverage disclosed by the invention is high in stability, high in nutritional value, fine and smooth in taste, pleasant in flavor, suitable for people who lose weight, people with hypertension and people with low immunity, and capable of creating better economic benefits.

This invention relates to a method for producing chondroitin with improved safety and extracellular secretion levels, belonging to the field of genetic engineering. Using the probiotic *Escherichia coli* Nissle 1917 as the host, this invention designs methods to increase chondroitin production by studying the chondroitin synthase KfoAC and the chondroitin-linking Kdo synthase. The relationship between chondroitin synthesis and secretion in *E. coli* was clarified, and the specificity of the chondroitin-linking Kdo was investigated, resulting in an extracellular chondroitin production of 0.32 g / L. Subsequently, the introduction of the protein KfoB from the chondroitin synthesis gene cluster increased the extracellular chondroitin production to 0.51 g / L. Further screening and optimization of the expression of transport proteins resulted in an extracellular chondroitin production of 0.73 g / L in shake flasks. Further optimization using a 7-L fermenter resulted in an extracellular chondroitin production of 5.9 g / L.

Provided herein are recombinant microorganisms that express a subject polypeptide. Microorganisms can comprise an expression construct comprising a flagellin promoter operatively linked with a heterologous nucleotide sequence encoding the subject polypeptide. The flagellin promoter sequence can comprise a genetic modification that reduces CsrA inhibition of translation. Microorganisms also can comprise a genetic modification that reduces FlgM inhibition of SigD initiation of transcription. The target polypeptide can be an aldehyde dehydrogenase. Such microorganisms are useful in the treatment of alcohol hangover.

A dry stable probiotic composition is provided. The composition comprises one or more viable probiotic microorganisms, one or more hydrolyzed proteins, one or more disaccharides, one or more oligosaccharides, and one or more polysaccharides, but not trehalose. The composition has viability of at least 1×1010 CFU / g, and a viability loss of less than 1 log unit / g after one month at a temperature of 40° C. and a relative humidity of 33%. Also provided are methods for preparing the dry stable probiotic composition.

The invention provides a preparation method of a probiotic fermented rice cake containing prebiotics and metabiotics. The preparation method comprises the following steps: firstly, adding rice flour, prebiotics, metabiotics and probiotics into water, uniformly mixing, carrying out primary fermentation for 1-24 hours, adding saccharomyces cerevisiae, uniformly mixing, carrying out secondary fermentation for 50-120 minutes, and steaming, so as to obtain the product. Wherein the mass ratio of the rice flour to the prebiotics to the metabiotics is 500: (100-300): (5-10). According to the preparation method disclosed by the invention, a three-element synergistic functional system of prebiotics, probiotics and metabiotics is constructed, so that the prepared probiotic fermented rice cake has full appearance and moderate bulkiness, texture key indexes such as hardness and elasticity of the probiotic fermented rice cake can be remarkably optimized, a better eating mouth feel is obtained, the rice cake is endowed with better texture characteristics, and the probiotic fermented rice cake is suitable for being eaten by people. Besides, the flavor of the rice cake can be enriched, the rice cake is endowed with coordinated and pleasant fermentation flavor, and then the sensory quality of the rice cake is synergistically improved.

The invention discloses pediococcus acidilactici and application thereof in preparation of a medicine for preventing or treating diarrhea of yak calves, and belongs to the technical field of microorganisms. The pediococcus acidilactici is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M20252012, and the preservation date is September 12, 2025. The strain has remarkable bacteriostatic activity on yak-derived multi-drug-resistant escherichia coli (the accession number of NCBI is PP859186). After the probiotic preparation is orally taken, the yak calf diarrhea induced by escherichia coli can be effectively relieved, the diarrhea rate and the inflammatory factor level are remarkably reduced, the oxidation resistance is improved, the flora structures of intestinal bacteria and fungi are adjusted, and the micro-ecological balance is restored. The invention provides a safe, green and efficient alternative antibody product which is suitable for the plateau yak breeding industry.

The invention belongs to the technical field of microorganisms, and relates to a bifidobacterium breve strain capable of relieving constipation, the strain is bifidobacterium breve Bb-PG-14, the classification name of the strain is Bifidobacterium breve, the preservation number is CGMCC No.31861, the preservation time is September 4, 2024, the preservation unit is China General Microbiological Culture Collection Center, the preservation number is CGMCC No.31861, the preservation number is CGMCC No.31861, the preservation number is CGMCC No.31861, the preservation number is CGMCC No.31861, the preservation number is CGMCC No.31861, and the preservation number is CGMCC No.31861. The address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain is separated and purified from excrement of healthy babies, has relatively strong acid resistance, alkali resistance, cholate resistance and artificial simulated gastrointestinal fluid tolerance, and has the characteristics of probiotics.

The invention relates to a probiotic product stability prediction model and a construction method and application thereof. The invention provides a new method capable of predicting the stability of the probiotic product earlier, faster and more accurately, 14 potential influence factors such as strain physiological characteristics, preparation processes and environmental factors are incorporated into a consideration system, and a machine learning algorithm is utilized to capture a complex nonlinear relationship between the potential influence factors and the stability, so that the stability of the probiotic product can be predicted more early, faster and more accurately. The accuracy and the reliability of the prediction model are greatly improved, the prediction timeliness is high, the universality and the guidance are high, and digital management can be realized.

The invention relates to the field of traditional Chinese medicine beverages, and discloses a winter traditional Chinese medicine beverage with a lung moistening function and a preparation method thereof.The winter traditional Chinese medicine beverage is prepared from, by weight, 8-12 parts of lily extract, 5-8 parts of almond peptide, 2-4 parts of mogroside, 3-5 parts of tremella polysaccharide, 0.5-1 part of pericarpium citri reticulatae volatile oil and 1-2 parts of bulbus fritillariae cirrhosae slow-release microcapsules. The invention relates to a low-temperature enzymolysis and supercritical CO2 extraction combined decoction, which is prepared from the following raw materials in parts by weight: 4-6 parts of a compound probiotic leavening agent, 3-5 parts of natural honey, 2-3 parts of fructo-oligosaccharide, 0.3-0.8 part of citric acid, 0.1-0.3 part of a natural flavor regulator and the balance of purified water, aims at the problem of extensive traditional decoction process, and realizes accurate treatment of different medicinal material characteristics through a low-temperature enzymolysis and supercritical CO2 extraction combined technology. According to the present invention, the high temperature is prevented from damaging the thermosensitive component, the extraction efficiency of the insoluble substance is improved through the pressure regulation, the retention rate of the active component is qualitatively improved, and the composite probiotic fermentation process is innovatively introduced in the systematicness of the compatibility.

The invention relates to the field of animal nutrition additives, and particularly discloses an animal additive based on probiotic synergistic fermentation sea buckthorn residue and hermetia illucens protein and a preparation method thereof, the animal additive comprises the following components by mass: 5%-10% of a filling auxiliary material, and the balance of a fermentation composite product; the sea-buckthorn residues and the hermetia illucens larvae can be fermented to form a double-substrate fermentation product, so that the double-substrate fermentation product has a synergistic nutrition effect, and can be used for remarkably promoting the weight gain condition of animals, improving spleen and thymus indexes and increasing serum IgG and IgM; the hermetia illucens protein is rich in lauric acid, antibacterial peptide and high-quality animal protein, so that the non-specific immunity and the anti-stress capability of an organism are enhanced, and the infection risk in the breeding process is reduced; the low-value sea-buckthorn residues and the hermetia illucens larvae are upgraded and converted into high-value feeding resources in a biological fermentation mode, the environmental pressure of processing by-products is relieved, the additional value of raw materials is increased, and the method conforms to resource circulation and environmental protection policy guidance.