688 results about "Probiotic" patented technology

The invention provides a probiotics plant solid beverage for treating hyperuricemia and gout. The solid beverage is prepared by mixing freeze-dried probiotics powder and plant extract with the addition of proper auxiliary materials. The solid beverage comprises the following components: the plant extract, the auxiliary materials and the probiotics, with the sum of the mass percentage of each component being 100%. The plant extract comprises celery seed powder, sophora flower bud powder and platycodon root powder. The invention has an obvious effect on lowering uric acid and relieving gout.

The invention discloses a probiotic-polyphenol nanoparticle colon-targeted co-delivery system as well as a preparation method and application thereof, and belongs to the field of biological medicines. The FCPs and hyaluronic acid (HA) are combined through non-covalent interaction, so that the hydrogel has colon targeting property and mucosal adhesion at the same time, and delivery of probiotics is facilitated. The hydrogel (HF) based on polysaccharide has good biocompatibility, and can be used for effectively encapsulating the probiotics and the natural products. Then, the PDA-TH NPs and BA are incorporated into the polysaccharide chain network of HF, and finally the BA-HF-PDAT targeted co-delivery system is constructed. The BA (at) HF-PDAT targeted co-delivery system can protect BA from serious gastrointestinal stress, and is jointly assembled with PDT-TH NPs to realize dual slow release of thymol (Thy), so that the treatment effect of BA and Thy is improved, and the BA (at) HF-PDAT targeted co-delivery system contributes to treatment of clostridium difficile infection (CDI).

The invention relates to the field of microorganisms, and discloses an aspergillus cristatus strain and application thereof. The preservation number of the aspergillus cristatus provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.41727. The aspergillus cristatus provided by the invention can produce chymosin at high yield, has excellent curding activity and relatively low proteolytic activity, and can reduce the bitter taste of dairy products and improve the yield; the aspergillus cristatus provided by the invention not only can shorten the fermentation time of the dairy product, improve the cream flavor of the dairy product, reduce the peculiar smell of the dairy product, balance the sourness and sweetness of the dairy product and improve the cinnamon flavor, but also has intestinal colonization ability, and has the potential of improving the flavor level of the dairy product and becoming intestinal probiotics.

The invention is applicable to the technical field of daily cleaning chemicals, and provides a double-shell probiotic microcapsule which comprises probiotics, a fat-soluble plant extract, cellulose nanocrystals, a polyacrylic acid-chitosan compound, a surfactant, diisocyanate, polyethylene glycol and ethyl acetate. A wall material of the microcapsule is formed by compounding a polyacrylic acid-chitosan compound and a fat-soluble plant extract, the particle size D50 is smaller than or equal to 5 microns, the microcapsule is stable in an environment with the pH being 8-10, and the microcapsule is broken to release probiotics when the pH is smaller than or equal to 6. The outer layer is polyacrylic acid / chitosan compound / polyurethane crosslinking (pH response), the inner layer is coconut oil and soybean oil (fat-soluble core), meanwhile, the material preparation process is simple, the controllable degree is high, and the material is suitable for batch preparation.

The invention belongs to the technical field of engineered probiotics, and particularly relates to engineered probiotics for radiosensitization and tumor metabolism regulation as well as a preparation method and application of the engineered probiotics. The engineering probiotics comprise probiotics and core-shell type metal nanoparticles which are loaded on the probiotics and are synthesized through a biological directional mineralization effect; the core of the core-shell type metal nanoparticle is a palladium element, and the outer layer of the core-shell type metal nanoparticle is a palladium element and a gold element. The engineering probiotics provided by the invention have excellent enzyme catalysis performance and can target and retain in tumor sites, and through the synergistic effect of all components in the engineering probiotics, adenosine metabolism is effectively inhibited, secretion of pro-inflammatory cytokines is enhanced, tumor microenvironment is promoted to be converted into an inflammatory state, up-regulation of immune checkpoints is inhibited, and the immune checkpoints are inhibited. And T cells are inhibited from being transformed into depletion phenotype and transformed into effect type from depletion type. After the SBRT and the immune checkpoint inhibitor are combined for use, the growth of tumors can be effectively inhibited, and the effect of enhancing immune radiotherapy is achieved.

The invention relates to the field of fermentation compounds, in particular to a compound for promoting GLP-1 secretion, a fermentation preparation method and application of the compound. According to the compound, fermented mulberry leaf powder is used as a main material, soybean oligosaccharide and rhizoma kaempferiae rhizome extract are used as synergistic materials, white birch juice concentrate and white kidney bean extract are used as auxiliary materials, the mulberry leaf powder is subjected to compound probiotic fermentation and multi-stage directional enzymolysis to enrich active components, and meanwhile, the soybean oligosaccharide is extracted and purified by adopting ultrasonic-assisted extraction in combination with a gradient alcohol precipitation method. Supercritical CO2 extraction is assisted to obtain a high-purity rhizoma kaempferiae rhizome active extract; finally, all the extracts are compounded in proportion and embedded through sodium alginate-chitosan wall material microcapsules, and the stability is improved with the assistance of a vitrification carrier and an antioxidant; the composition provided by the invention has a multi-target synergistic effect, and can significantly promote the secretion of GLP-1.

The invention provides a novel enterococcus strain Enterococcus qinghaiensis SCFF543 and an anti-oxidation application of the novel enterococcus strain Enterococcus qinghaiensis SCFF543, and belongs to the technical field of microorganisms. Wherein the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on June 10, 2025, and the preservation number is CGMCC No.34843. The invention further discloses a preparation method of the strain. The strain is separated from homemade casein of herdsman families in Demaoha City in Haixi, Qinghai province, has the advantages of strong antioxidant capacity, no vancomycin resistance, strong adhesiveness and the like, and can be suitable for preparing probiotic products with antioxidant effects.

The invention belongs to the field of feeds, and discloses a biological feed containing a microbial microcapsule preparation and a preparation method of the biological feed. The biological feed is prepared from the following components in parts by weight: 90 to 93 parts of wall-broken rhodopseudomonas palustris powder, 2 to 3 parts of lysine hydrochloride, 0.1 to 0.5 part of methionine, 0.1 to 0.5 part of threonine, 0.3 to 1 part of arginine, 2 to 3 parts of calcium hydrophosphate, 1 to 2 parts of calcium carbonate, 0.5 to 1.2 parts of an antioxidant additive, 0.3 to 0.8 part of modified nano montmorillonite and 1 to 5 parts of a microbial microcapsule preparation. According to the biological feed, the wall-broken rhodopseudomonas palustris serves as basic nutrition and a main microorganism source of the feed and is matched with the compound probiotics of the microorganism micro-capsule preparation, the intestinal nutrition utilization rate is effectively increased, meanwhile, the antioxidant additive is added, and the problems that a single additive is narrow in function and prone to oxidation are solved; the modified nano montmorillonite is added to solve the problem of antagonism of toxin adsorption and mineral absorption.

The invention relates to the technical field of microbiological detection, in particular to active probiotic component detection equipment which comprises a box body, a microscope is arranged in the box body, a glass slide is arranged below the microscope, a cover glass is placed at the top of the glass slide, a box door is rotationally connected to one side of the box body through a hinge, a placement plate is fixedly mounted in the box body, and sliding grooves are formed in the two sides of the placement plate. A clamping unit is slidably connected in the sliding groove, a glass slide is fixedly connected above the placing plate through the clamping unit, a first mounting groove is formed in one end, close to the glass slide, of the placing plate, a driving unit is fixedly connected in the first mounting groove, a separating unit is arranged above the glass slide, and a heating unit is arranged below the glass slide; the separation unit and the heating unit are both controlled by a driving unit, the bottom end of the clamping unit penetrates through the placing plate to be fixedly connected with a moving unit, a dyeing unit is arranged in the end, away from the clamping unit, of the placing plate, and an angle adjusting unit is arranged below the dyeing unit.

The invention provides a cobalt monatomic nano-enzyme system modified by phospholipid polyethylene glycol carboxyl. The cobalt monatomic nano-enzyme system comprises a cobalt monatomic nano-enzyme composite core and phospholipid polyethylene glycol carboxyl compounded on the surface of cobalt monatomic nano-enzyme, the cobalt monatomic nano-enzyme composite core comprises a cobalt monatomic nano-enzyme and ginsenoside loaded on the cobalt monatomic nano-enzyme. The invention also provides a bifidobacterium synergetic cobalt monatomic nano-enzyme system which is formed by combining cobalt monatomic nano-enzyme with ginsenoside Rb1 and further delivering the cobalt monatomic nano-enzyme and ginsenoside Rb1 through bifidobacterium bifidum for targeted therapy of enteritis. The combination of Co SA and Rb1 can enhance the anti-inflammatory efficacy by adjusting a key immune signal pathway, and bifidobacterium bifidum can ensure targeted delivery of microbiota and promote intestinal barrier repair. The synergistic three-component strategy integrating monatomic nano-enzyme antioxidation, natural drug immunoregulation and probiotic targeting is provided, and a promising treatment platform is provided for accurate intervention of IBD.

The invention discloses an ecological planting method for remarkably improving the amino acid content of citrus, which comprises the following steps: (1) mixing and fermenting pruned citrus branches, weed straws, livestock and poultry manure and indigenous probiotic ferment powder to prepare a basic microbial fertilizer, and adding a calcium magnesium phosphate fertilizer and monopotassium phosphate to prepare a special microbial fertilizer for citrus, applying as a base fertilizer in winter; (2) after citrus pruning, in key phenological periods such as a flower bud period, a young fruit period and a fruit swelling period, an indigenous probiotic enzyme diluent with a specific dilution ratio is used for systematic foliage spraying on a tree body. The indigenous probiotic enzyme contains bacillus amyloliquefaciens, saccharomycetes, active enzymes and a plurality of nutrient elements. The method can significantly increase the total amino acid content of citrus fruits, greatly increase the yield, improve the taste of the fruits, enhance the tree body resistance, reduce the use of chemical pesticides, improve soil, and realize quality improvement, yield increase and green sustainable development of citrus planting.

The invention belongs to the technical field of traditional Chinese medicine fermentation, and particularly relates to a traditional Chinese medicine fermentation composition for regulating sleep as well as a preparation method and application thereof. The traditional Chinese medicine fermented composition for regulating sleep is prepared by fermenting spina date seeds, poria cocos, lily, liquorice, polygala tenuifolia, cortex albiziae, lotus seeds and Chinese yams serving as traditional Chinese medicine raw materials through probiotics. The traditional Chinese medicine raw materials with specific components are selected and subjected to gradient fermentation with specific strains, the prepared traditional Chinese medicine composition is remarkable in sleep adjusting effect, and the level of 5-hydroxytryptamine can be remarkably improved.

The invention relates to the technical field of microbial fermentation, and discloses a metabolite fingerprint spectrum construction method of a probiotic fermentation type Chinese herbal medicine preparation. The method is realized by sequentially executing a metabolite data acquisition process, a chromatographic data preprocessing process, a metabolite fingerprint spectrum modeling process, a spectrum construction optimization process and a metabolite fingerprint spectrum generation process. In the metabolite data acquisition process, original metabolite data of the probiotic fermented Chinese herbal medicine preparation is acquired; in the chromatographic data preprocessing process, original metabolite data is subjected to signal processing and feature extraction, and preprocessed chromatographic data is obtained; in the metabolite fingerprint modeling process, a preliminary fingerprint is constructed based on the preprocessed chromatographic data; in the atlas construction optimization process, parameter optimization and accuracy improvement are carried out on the initial fingerprint atlas, and the optimized fingerprint atlas is obtained; in the metabolite fingerprint spectrum generation process, the optimized fingerprint spectrum is integrated, and a final metabolite fingerprint spectrum is generated.

The invention belongs to the technical field of biology, and particularly relates to a synthetic probiotic phenotypic characteristic prediction method and system.The method comprises the steps that a genome is divided into gene units, K-mer sequences containing position weights and codon coding information are extracted from the gene units, and an initial characteristic vector is constructed for each strain; by calculating mutual information between each K-mer feature and a known phenotype, identifying which gene segments are most critical for predicting a target phenotype, and distributing significance weights for the gene segments; after the feature vectors of the known strains are optimized and weighted through the weights, a machine learning prediction model is trained; and when a new synthetic probiotic needs to be predicted, executing the same feature extraction and weighting process on the genome of the new synthetic probiotic, and inputting the obtained vector into the trained model to obtain a prediction result of the phenotypic features of the new synthetic probiotic.

The invention relates to the technical field of biological preparations, and discloses an efficient body function repairing active small molecule peptide preparation and a preparation method thereof.The efficient body function repairing active small molecule peptide preparation is prepared from 15-22 parts of collagen peptide, 5-9 parts of glutathione, 10-18 parts of whey protein peptide, 5-8 parts of vitamin C, 2-4 parts of vitamin E, 1-3 parts of zinc element, 5-15 parts of astragalus membranaceus extract, 3-8 parts of probiotics and 10-20 parts of a delivery carrier. The molecular weight range of the original protein peptide, the glutathione and the whey protein peptide is 500 to 2000Da. According to the invention, collagen peptide, glutathione, whey protein peptide, vitamins and other components are proportioned to realize an anti-oxidation-repair-anti-inflammatory synergistic effect, and vitamin C, vitamin E, zinc element and an astragalus extract are added to cover multi-dimensional repair of an immune system, so that comprehensive body function repair is realized.

The invention relates to the technical field of microbial fermentation, and discloses an agricultural ecological probiotic complex microbial inoculant, which is prepared from the following components in percentage by mass: 30 to 35 weight percent of beneficial microbial inoculant, 20 to 25 weight percent of mineral source potassium fulvate, 15 to 20 weight percent of slow release agent, 10 to 15 weight percent of nano polypeptide fermentation liquor and the balance of carrier, the processing method comprises the following steps: S1, pretreating the raw materials; s2, preparing nano polypeptide fermentation liquor; s3, complex microbial inoculants are compounded; and S4, drying and forming. According to the invention, the mineral source potassium fulvic acid and the nano-polypeptide fermentation liquor are added, so that effective regulation and control of soil micro-ecology are realized; crop growth is promoted through cooperation of multiple strains, growth hormone is secreted through bacillus subtilis and the like, nitrogen is fixed through bacillus amyloliquefaciens, root development is stimulated in cooperation with mineral source potassium fulvic acid, and the plant height, stem diameter and fresh weight of crops are remarkably increased; the particle size of the polypeptide subjected to high-pressure homogenization treatment is 80-120nm, the absorption efficiency is improved by 60%, and a crop disease-resistant signal channel is activated.

The invention discloses a single-cell probiotic packaging method of thiolated guluronic acid oligosaccharide and a probiotic microcapsule, and relates to the technical field of biological medicines. The packaging method comprises the following steps: mixing a thiolated guluronic acid oligosaccharide solution with a probiotic suspension to form a probiotic-polysaccharide mixed solution; and adding a calcium chloride solution into the probiotic-polysaccharide mixed solution, and stirring to form the single-cell microcapsule encapsulating the probiotics. The packaging method can protect a single strain from being damaged by an external adverse environment to the maximum extent, and the survival rate is increased. Meanwhile, the obtained single-cell microcapsule can improve the digestion of the probiotics, the survival rate of the probiotics under the oxidative stress environment and the antibiotic combination, has high stability, and prolongs the intestinal residence time of the probiotics. An innovative solution is provided for the problems that in the existing medicine field, probiotic preparations cannot improve the gastrointestinal stress resistance of probiotics, and antibiotics lose efficacy when combined.

The present invention discloses a CBP lysine children growth milk powder and a preparation method thereof, and belongs to the technical field of milk powder, the CBP lysine children growth milk powder comprises the following components by mass: a wet process part: 575 parts of whole milk powder, 175 parts of dried skim milk, 175 parts of isomaltooligosacharide (type 50) powder, 30 parts of polydextrose, 28 parts of edible plant blend oil, 4 parts of milk mineral salt, and 13.7 parts of a compound children modified milk powder three-nutrition enhancer; cBP lysine is cooperated with bifidobacterium and seven traditional Chinese medicine extracts, DHA and ARA are accurately proportioned, the calcium absorption efficiency and the intestinal colonization capacity of probiotics are remarkably improved, a three-step mixing method is combined with a two-way shearing low-temperature technology, rapid and uniform mixing is achieved, the activity of the probiotics and the stability of DHA are reserved to the maximum extent, high uniformity of products is ensured, and the product quality is improved. The low-moisture wet-process base powder inhibits moisture absorption of traditional Chinese medicine components, nitrogen protection blocks oxidation in the whole process, the shelf life is prolonged, and the reconstituability is improved.

The present invention generally provides methods and compositions for the treatment of Parkinson's disease and depression and / or anxiety. The invention relates to recombinant microorganisms, particularly gut-colonizing probiotics, modified to produce L-DOPA.

The invention belongs to the field of biotechnology and nutritional medicine, relates to a maternal obesity intervention preparation, and particularly relates to a preparation method of a plant embryo fermentation product and application of the plant embryo fermentation product in blocking of trans-generation delivery metabolic diseases. FWG is prepared through a probiotic fermentation technology, obesity, glucose and lipid metabolism disorder and oxidative stress induced by high-fat diet of a parent body can be remarkably improved, and cross-generation transmission of metabolic diseases is blocked by adjusting composition of intestinal flora of the parent body and offspring and a hypothalamic PI3K-Akt signal channel. The invention reveals that maternal FWG intervention passes through a mechanism that a PI3K-Akt pathway is dominated and multiple pathways are coordinated on the transcriptome level for the first time, the HFD-induced offspring hypothalamus metabolism programming abnormality is reversed, and a new central regulation target is provided for cross-generation metabolic disease prevention. The FWG is a wheat processing byproduct, is free of chemical additives, is suitable for long-term dietary supplement, is a natural and safe nutrition intervention scheme, and is suitable for the field of maternal and infant health.

The invention discloses a compound rumen bypass guanidinoacetic acid additive as well as a preparation method and application thereof, and belongs to the technical field of animal feed additives. The compound rumen bypass guanidinoacetic acid additive provided by the invention is prepared from the following components in parts by mass: 40 to 60 parts of guanidinoacetic acid, 0.1 to 0.5 part of selenium yeast, 5 to 10 parts of nicotinamide, 5 to 12 parts of DL-methionine, 0.01 to 0.05 part of vitamin B12, 1 to 3 parts of compound probiotics, 3 to 5 parts of hydroxypropyl methyl cellulose, 1 to 2 parts of ethyl cellulose, 15 to 25 parts of hydrogenated palm oil and 3 to 5 parts of glyceryl monostearate. The compound probiotics comprise bacillus subtilis and saccharomyces cerevisiae. The compound additive provided by the invention can significantly improve the daily gain of ruminants and reduce the feed-gain ratio, and meanwhile, due to the improvement of the energy utilization efficiency and the enhancement of the antioxidant ability, the immunity of animals is improved, the morbidity is reduced, and the meat quality is improved.

The invention discloses a breeding method for improving pork quality by using Chinese herbal medicine fermentation broth, and relates to the field of livestock and poultry breeding, the method comprises the following steps: preparing composite Chinese herbal medicine fermentation broth, and adding the fermentation broth into daily ration in proportion for feeding in the growth and fattening stage of live pigs, the compound Chinese herbal medicine fermentation liquor is prepared by fermenting a Chinese herbal medicine composition containing astragalus membranaceus, pine needles, eucommia ulmoides and hawthorn through a compound microbial agent; according to the breeding method for improving the pork quality by using the Chinese herbal medicine fermentation liquor, Chinese herbal medicines such as astragalus membranaceus, pine needles, eucommia ulmoides and hawthorn fruits are fermented through a compound microbial agent, the deep synergistic effect of the Chinese herbal medicines and probiotics is achieved, the bioavailability of effective components is remarkably improved, and therefore products better meeting high-end market requirements are produced; meanwhile, probiotics and metabolites in the fermentation liquor can effectively optimize the intestinal health of livestock and reduce the morbidity, and a reliable path is provided for reducing or replacing antibiotics.

The invention relates to the technical field of biopesticides, in particular to a compound preparation for preventing and treating meloidogyne incognita as well as a preparation method and application of the compound preparation. The compound preparation for preventing and treating meloidogyne pinnatifida comprises the following raw materials in percentage by mass: 0.1-5% of azadirachtin or compound azadirachtin, 30-60% of castor seed meal, 35-65% of activated carbon and the balance of an adhesive. The active carbon, the azadirachtin and the castor seed meal are compounded, and the azadirachtin realizes efficient contact killing by inhibiting nematode respiratory chains and neural signal transmission and has no drug resistance; the active carbon is used as a slow-release carrier to prolong the pesticide effect (lasting for more than 90 days), and can adsorb soil toxins to improve the microenvironment; the castor seed meal can provide organic matters to promote the proliferation of soil probiotics and improve the soil structure on one hand, and is rich in ricin on the other hand, so that the hatching of nematode eggs can be effectively inhibited; the three components are combined to realize synergistic interaction through three mechanisms of slow release, killing and improvement.

The invention discloses a biochar-based microbial soil conditioner and a preparation method thereof, and relates to the technical field of soil improvement. The preparation method comprises the following steps: firstly, carrying out alkali-acid treatment and segmented pyrolysis on raw materials such as wood chips to obtain primary biochar, and then carrying out chemical activation, nano material modification and plasma treatment to obtain the composite modified biochar. Meanwhile, probiotics such as bacillus subtilis and the like are subjected to activation, expanding culture and saline-alkali domestication to obtain directional domestication bacterial liquid, and the directional domestication bacterial liquid is solidified into microspheres by adopting a composite embedding carrier. And finally, mixing the composite modified biochar, the composite embedded microspheres, potassium humate and other auxiliary materials, drying, and sieving to obtain the soil conditioner. The soil conditioner disclosed by the invention can realize an improvement effect on saline-alkali soil and reduce the total salt content; meanwhile, directionally domesticated microorganisms secrete organic acid to neutralize alkaline components, the pH of the soil is adjusted to the range suitable for crop growth, and salt and alkali stress is relieved.

The invention discloses a shrimp seed breeding method for regulating and controlling water quality through microalgae and probiotics under specific illumination. According to the technical scheme, the shrimp seed breeding method is characterized by comprising the following steps that firstly, a seedling breeding workshop is built; step 2, microalgae-probiotic culture: selecting microalgae and probiotic types beneficial to shrimp seedling culture, and culturing to obtain a microalgae solution and a probiotic solution; 3, seedling raising and putting preparation; step 4, putting shrimp larvae; step 5, putting of algae-probiotics; step 6, seedling culture illumination control; 7, feeding the young shrimps with bait; 8, monitoring and regulating water quality; and 9, evaluating the young shrimps out of the pond. According to the method, illumination is precisely controlled, the growth environment of microalgae and probiotics is optimized, and the synergistic effect of the microalgae and probiotics in the aspects of water purification, nutrition supply, disease prevention and control and the like is fully exerted, so that the water quality of penaeus vannamei larvae is improved, the survival rate and health condition of the penaeus vannamei larvae are improved, and the survival rate of penaeus vannamei larvae is increased. And a good foundation is laid for large-scale and high-efficiency culture of the penaeus vannamei.

The invention belongs to the technical field of biology, and particularly discloses a pH response type probiotic-liposome hybrid system, a preparation method and application thereof, and the pH response type probiotic-liposome hybrid system comprises engineered escherichia coli Nissle 1917, pinocembrin-loaded biotinylated liposome and an enteric polymer; wherein the engineered escherichia coli Nissle 1917 is in non-covalent binding with the biotinylated liposome loaded with the pinocembrin through surface display streptavidin, so that EcNPIN is formed, and the EcNPIN is coated with an enteric polymer, so that the pH response type probiotic-liposome hybrid system is formed. The pH response type probiotic-liposome hybrid system EcNPIN-L provided by the invention can resist the acidic environment of the stomach, completes pH response release and colonization in the intestinal tract, solves the problems of lack of intestinal tract localization of drugs and low probiotic treatment efficiency, improves the local intestinal tract concentration of pinocembrin, and promotes intestinal tract colonization of probiotics.

The invention relates to the field of probiotic fermentation, and discloses a probiotic fermented beverage prepared from sea buckthorn fruit peptide and morinda citrifolia fruit peptide and a preparation method thereof, and the probiotic fermented beverage is prepared from the following components by weight: 10-15 parts of a sea buckthorn fruit peptide extract, 8-12 parts of a morinda citrifolia fruit peptide extract, 5-8 parts of a composite probiotic starter, 3-5 parts of fructo-oligosaccharide, and 1-2 parts of a natural sweetener. According to the present invention, the sea buckthorn fruit peptide and the morinda citrifolia fruit peptide are scientifically compounded, such that the synergistic interaction of the two plant active components is achieved, the double-fruit peptide matrix provides the differentiated nutrition supply for the composite probiotics, and the fermentation efficiency is improved through the nutrition complementation; according to the present invention, with the fermentation medium, the growth requirements of different strains are met, the proliferation efficiency and the metabolic activity of the probiotics are significantly improved, the industry problems of poor strain adaptability and low viable count in the traditional single-substrate fermentation are solved through the innovative compatibility, and the fermentation process is stable and efficient.

A food medicine for reducing blood uric acid and treating gout is characterized in that celery seeds, celery, mulberry fruits, mulberry leaves, sea buckthorn fruit powder, dandelions, rhizoma polygonati, poria cocos and beach kidney grass serve as a basic formula; the auxiliary materials comprise one, several or all of a plurality of formula combinations of ramulus mori, lycium ruthenicum, radix puerariae, Chinese yam, clerodendranthus spicatus, lysimachia christinae hance, acanthopanax, anserine, astragalus membranaceus, purslane, herba cepbalanoplosis segeti, galangal, hovenia dulcis thunb, astragalus membranaceus, luteolin, uric acid-reducing edible probiotics and the like, and are used for treating hyperuricemia and gout.

The technology described herein is directed to probiotic microbes engineered to secrete at least one enteroendocrine modulating polypeptide, which can be a sweet protein. In some embodiments, the probiotic microbe secretes the enteroendocrine modulating polypeptide in the distal colon. Also described herein are nucleic acids or vectors comprising a coding sequence for the enteroendocrine modulating polypeptide. Also described herein are compositions comprising the engineered probiotic microbe. Methods of using such probiotic microbes and compositions are also disclosed, including methods for modulating enteroendocrine cells and methods for treating metabolic diseases.

The invention provides a probiotic screening method based on a fine tuning DNABERT model and a convolutional neural network. The method comprises the following steps: acquiring a reference genome sequence and a target sequence sample; preprocessing the reference genome sequence and the target sequence sample; segmenting the preprocessed reference gene sequence, and inputting the segmented reference gene sequence into a DNABERT model for fine tuning training; expanding the number of samples of the preprocessed target sequence, segmenting the sequence by using a sliding window, and inputting the segmented sequence into the fine-tuned DNABERT model for decoding to obtain a representation vector; inputting the representation vector into a CNN model for training, sequencing a target sequence to obtain a to-be-detected sequence sample, encoding the to-be-detected sequence sample, inputting the encoded to-be-detected sequence sample into the CNN model, judging whether the strain is the probiotics or not, and completing screening of the probiotics; according to the method, the data enhancement technology of introducing the reverse complementary sequence and the randomly intercepted sub-sequence is adopted, so that the diversity and the quantity of training data are increased, and the overfitting problem is effectively relieved.