The invention relates to a chicken bone fungi sausage and a preparation method of the chicken bone fungi sausage. The chicken bone fungi sausage is characterized by being prepared by the following raw materials in parts by weight: 80-90 parts of ass meat, 2-3 parts of refined salt, 1-2 parts of monosodium glutamate, 3-4 parts of maltose, 2-3 parts of light soy sauce, 1-2 parts of white vinegar, 0.7-1 part of citric acid, 2-3 parts of thick broad-bean sauce, 10-12 parts of chicken bones, 6-8 parts of red bean, 6-8 parts of straw mushrooms, 7-8 parts of mushrooms, 4-5 parts of hedgehog fungus, 4-5 parts of lily, 4-6 parts of vetch, 5-6 parts of lysimachia crispidens, 2-3 parts of chrysanthemum, 3-4 parts of pumpkin flowers, 1-2 parts of seabuckthorn, 4-5 parts of mulberries, 2.5-3 parts of cardamine, 1-2 parts of lychee seeds, 1-2 parts of tea-seed oil, 1-2 parts of honey, 0.15-0.2 part of cellulose and 0.2-0.3 part of protease. The chicken bone fungi sausage is rich in nutrition, unique in flavor and convenient to eat, and has the functions of tranquilizing by nourishing the heart, moistening lung to arrest cough, prolonging life, eliminating dampness and heat, mitigating edema and scattering stasis, resisting and preventing cancers, invigorating stomach to help digestion, reducing blood pressure and stopping bleeding by astringency.