Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Extraction method of cardamine hupingshanesis plant selenoprotein

A pot-pot crushed rice chestnut and plant selenoprotein technology, which is applied in the field of pot-pot crushed rice chestnut plant selenoprotein, can solve the problems that it is not suitable for large-scale production, etc.

Active Publication Date: 2014-02-19
白宏锋
View PDF2 Cites 19 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regarding the extraction of plant selenoproteins, there is no effective method suitable for large-scale production at present, and the present invention relates to a method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting plant selenoproteins of crushed rice chestnuts in a pot and a bottle, comprising the following steps in sequence,

[0025] (1) Crush the broken rice chestnut in the pot and degrease it, and then dry it;

[0026] (2) Soak the broken rice chestnut in the pot and bottle with 75% alcohol solution for 1 hour at 60±5°C to remove impurities such as small molecular sugars, metal ions and inorganic selenium;

[0027] (3) Filtrate the mixture obtained after soaking, remove the alcohol solution, and dry to obtain the broken rice chestnut powder in pots and bottles;

[0028] (4) Add an appropriate amount of water to the obtained pot and bottle of broken rice chestnut powder, the ratio of the pot and bottle of crushed rice chestnut powder to the added water is 1:8 (mass ratio), and carry out the water extraction operation at 60-80°C. Twice, each time for 1 hour, then filter and concentrate the water extract to obtain a concentrate; when concentrating the wat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an extraction method of cardamine hupingshanesis plant selenoprotein. The extraction method comprises the following steps: (1) cardamine hupingshanesis is degreased; (2) the cardamine hupingshanesis is dipped by 75% of an alcohol solution at the temperature of 60 minus 5 to 60 plus 5 DEG C; (3) a mixture obtained after dipping is filtered, so as to remove the alcohol solution, and powder is obtained by drying; (4) the obtained powder is added with water, the water extraction operation is performed at the temperature of 60-80 DEG C; water extraction liquid is filtered and concentrated to obtain concentrated liquid; (5) ethanol is added in the concentrated liquid, the volume of the added ethanol is 70% of the total volume of the solution, and an alcohol precipitate is obtained through still standing or filtering; (6) the alcohol precipitate is added with water, and then is added with pectinase, heat preservation is performed for 40 minus 5 to 40 plus 5 minutes, thermostable amylase is added, and heat preservation is performed for 30 minus 5 to 30 plus 5 minutes, so that hydrolysate is obtained; (7) the hydrolysate is added with ethanol, the volume of the added ethanol is more than 75% of the total volume of the solution, and precipitates are obtained through still standing or filtering; (8) the precipitates are dried. The extraction method is suitable for extracting the cardamine hupingshanesis plant selenoprotein in large scale.

Description

technical field [0001] The invention belongs to the technical field of plant protein extraction methods, and in particular relates to a method for extracting plant selenoproteins from crushed rice chestnuts in pots and bottles. Background technique [0002] Selenium is an essential trace element for the human body and plays an important physiological function in the human body. Inorganic selenium has cumulative toxicity and mutagenic effect, and the dose is difficult to control when used. Compared with inorganic selenium, selenoproteins have the advantages of low toxicity and high bioavailability. In recent years, the development and application of pure natural plant selenoproteins has become one of the research hotspots in animal nutrition at home and abroad. [0003] Huping broken rice chestnut is a new species of cruciferous rice chestnut plant unique to my country, which has a super selenium-enriching ability. Cultivated by ordinary artificial selenium supplementation...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K1/30
CPCC07K14/415
Inventor 白宏锋
Owner 白宏锋
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com