The invention discloses a method for extraction of
selenoprotein from
selenium-rich tea leaves. The method includes: taking
selenium-rich tea leaves as the raw materials, subjecting the
selenium-rich tea leaves to
drying, crushing and sieving, adding
ethanol, conducting heating in water bath at 60DEG C for 30min, performing
centrifugation to obtain precipitate, repeating the steps, and then carrying out
drying, crushing and sieving; adding
distilled water into the obtained precipitate, conducting freezing for 3-5h in a low temperature refrigerator, conducting heating in water bath at 50-70DEG C for 30min, then performing freezing in the low temperature refrigerator for 3-5h, conducting heating in water bath at 50-70DEG C for 30min, and repeating the operation three times; adding a NaOH solution, conducting heating in water bath at 70-90DEG C for 3-4h, performing
centrifugation under 3500rpm for 10min, repeating the process again on the precipitate, adjusting the pH of the supernatant to 2.5-3.5, carrying out standing,
centrifugation and
drying to obtain coarse
selenoprotein. The method provided by the invention has the advantages of simple preparation process, easy operation and environmental friendliness. The obtained product has high
selenoprotein content, and is a reliable source of organic selenium. Thus, the method is suitable for industrial production.