79 results about "Selenoprotein" patented technology

The invention discloses a bacterial selenoprotein online resource platform, an application method, a terminal and a medium, and relates to the technical field of biological medicine, the online resource platform is deployed on a server, the cloud server is a Ubuntu cloud server, and Nginx, Waitpress and Flask are configured; the server side is in butt joint with a background resource, and the background resource is in butt joint with the constructed bacterial selenoprotein database; the server is in butt joint with a user interface of the front end, the user interface designs a corresponding interface framework and an interaction function based on static resources hosted by Nginx, and the interaction function is used for realizing query, analysis and use of relevant information of the bacterial selenoprotein. According to the method, a convenient online access channel of an integrated database and a real-time data analysis tool are provided for users in related fields, and important support is provided for accurate annotation of selenoprotein genes in a bacterial genome plan.

The invention discloses an intelligent DYG selenoprotein liver nourishing, eye protecting and hangover alleviating gradient composite formula product and an intelligent wearable equipment health management system. DYG selenoprotein, dehydrogenase, pueraria flower refreshing soup, curcumin, lalang grass rhizome, allicin, vitamins and the like are compounded into a fast, medium and slow (long-acting) gradient liver nourishing, eye protecting and hangover alleviating formula, the liver nourishing, eye protecting and hangover alleviating formula has excellent oxidation resistance, detoxification and alcohol metabolism acceleration capabilities, can efficiently decompose alcohol, shorten drunkenness time and relieve discomfort after drinking, and eye protecting components are specially added to relieve eye fatigue. According to the invention, people drinking wine can supplement selenium for a long time, nourish liver and protect eyes into a normal state, and maintain overall health. The intelligent wearable device provides personalized health management, monitors physiological indexes in real time and pushes a customized alcohol dissolving scheme. The intelligent patch is small in design, portable and convenient to use. The comprehensive and personalized drinking health management requirements of users are met, long-term health of drinkers is concerned, and upgrading of the health industry is promoted. The invention has far-reaching social influence, wide market potential and remarkable social benefit, and aims to realize the goal of'smooth drinking without burden and health companion with each other '. The health of a drinker is taken as a core, the physical condition in the drinking process is deeply concerned, and unprecedented health experience is brought to the drinker. The invention aims at solving the global alcohol health chronic disease hidden behind the wine culture from the source by scientific and technological wisdom.

The present disclosure provides a preparation and application of high-activity and high-safety Cardamine violifolia selenoprotein standard sample. The standard sample of the Cardamine violifolia selenoprotein prepared by the present disclosure has the advantages of extremely low heavy metal content, less ash, high protein content, high total selenium content, high organic selenium ratio of up to 99.9%, high biological activity, high safety and so on; the organic selenium is mainly in form of selenocysteine / selenocystine and accounts for more than 90% of the total selenium content, and is expected to make up for the shortage of selenoprotein standard samples on the market.

The invention discloses an intelligent DYG selenoprotein NMN composite gradient anti-aging supplement product and an intelligent wearable equipment health management system, and a comprehensive and scientific anti-aging and intelligent health management system is constructed by integrating DYG selenoprotein, NMN and other core anti-aging components, combining various nutrients and traditional Chinese medicine components and integrating the traditional Chinese medicine massage and movement concepts. According to the scheme, through the synergistic effect of the composite components, the antioxidant capacity of the body is remarkably enhanced, damage of free radicals to cells is effectively reduced, and therefore the senescence process is delayed. Meanwhile, the application of the intelligent wearable device enables health management to be more personalized and accurate, and customized anti-aging strategies and health suggestions are provided by monitoring user health data in real time. In addition, health and longevity are promoted through multiple ways of adjusting energy metabolism, improving cardiovascular health, enhancing immunity and the like, and the life quality of the user is comprehensively improved. The combination of traditional Chinese medicine massage and exercise is not only beneficial to body health, but also capable of relieving psychological pressure and improving the overall happiness. On the whole, the anti-aging effect is remarkably improved, the disease prevention ability of the user is enhanced, and the healthy, longevity and vigor life experience is brought to the user.

The invention discloses a selenium-rich microecological preparation. The selenium-rich microecological preparation is prepared by the following steps: S1, activating strains; s2, preparing a selenomethionine premixed solution; s3, co-culture of bacteria and selenium; s4, forming a selenoprotein complex; s5, preparing a coating material; s6, coating with an emulsion; s7, acid-resistant layer coating; and S8, enteric activation treatment. According to different growth stages of the meat geese in the antibiotic-free breeding, a daily ration formula is directionally intervened: in the brooding period of the meat geese, the selenium-rich microecological preparation and daily ration feed are mixed in a dry mode, and conventional feeding is carried out; in the growth period of the meat geese, acidifying the selenium-rich microecological preparation, preparing the selenium-rich microecological preparation into a solution, and feeding the meat geese with the solution in the form of drinking water; in the fattening period of the meat geese, the premix containing the selenium-enriched microecological preparation is mixed with daily ration, and the meat geese are fed in an intensified mode. According to the invention, selenium-rich breeding of meat geese can be realized, economic benefits are increased, antibiotics can be replaced, and the eating safety and edible value of meat geese are improved.

The invention discloses a plant lactobacillus and application thereof, and belongs to the technical field of microorganisms. The plant lactobacillus 002P provided by the invention is a new strain, and is round, white, neat in edge, smooth in surface and soft in texture on an MRS solid culture medium; growth of bacteria can be remarkably inhibited, an inhibition effect on staphylococcus aureus, pseudomonas aeruginosa and escherichia coli is achieved, and the inhibition zone is 23.33-279.33 mm; the healing of infected wounds can be obviously promoted; the selenium conversion rate is excellent, and the maximum selenium conversion rate can reach 88%; the nano-selenium prepared from the plant lactobacillus 002P is spherical, has the particle size of 200-500nm, has excellent properties of resisting oxidation, relieving hepatotoxicity induced by saturated fatty acid, promoting expression of selenoprotein in a mouse body and reducing blood fat, and has a wide application prospect.

The present invention discloses a method for promoting the expression of selenoprotein genes in Chlamydomonas reinhardtii and increasing the yield of selenoproteins. S1. Inoculate Chlamydomonas reinhardtii algae with liquid culture medium at 20-30°C and 0-200 μmol / (m 2 S2, taking the Chlamydomonas reinhardtii culture solution, adding sodium selenite and abscisic acid, the abscisic acid concentration in the culture solution is 10-1000 μmol / L, at 20-30°C, 0-200 μmol / (m 2 ·s) Oscillating culture under light conditions. The present invention adds the plant hormone ABA during the selenium-enriched culture of Chlamydomonas reinhardtii, which not only does not affect the growth and biomass accumulation of microalgae, but also promotes the expression of selenoproteins. It is applied in the production of Chlamydomonas reinhardtii algae powder rich in selenoproteins, and can greatly increase the yield of selenoproteins.

The invention relates to the field of edible mushroom product production equipment, in particular to an edible mushroom selenoprotein extraction device and method.The edible mushroom selenoprotein extraction device comprises a tank body, a tank bottom and a bottom pipe, the tank bottom is arranged at the bottom of the tank body, the tank bottom and the tank body form a cylinder structure, one side of the tank bottom inclines downwards, and the bottom pipe is communicated with the downward side of the tank bottom; the outer side of the bottom pipe is communicated with a material guiding pipe, a discharging pump is arranged between the material guiding pipe and the bottom pipe, and the outer side of the material guiding pipe is communicated with a material conveying barrel; and the rear end part of the material conveying barrel extends upwards in an inclined manner. Through a positioning wheel, a torsional spring and a rotating lug hinge structure of the stirring rod, the stirring rod is elastically attached to the inclined tank bottom and swings in a self-adaptive mode, an arc-shaped swing piece is matched to drive materials to turn over up and down, deposition dead angles are eliminated, contact between disintegrating slag and an extracting agent is promoted, and selenium protein dissolution uniformity is improved; staggered holes of the fixed spiral and the movable spiral form a spiral filtering channel, and when waste residues are conveyed in a rotating mode, extrusion percolation is conducted synchronously to achieve residual liquid recovery.

The application relates to a method for extracting ergothioneine and selenoprotein from spirulina, wherein after culturing FACHB-Se1500 strains under the above-mentioned nitrogen deficiency induction condition, a product is obtained, and after purification, selenoprotein containing ergothioneine is obtained; the FACHB-Se1500 strains are cultured in a Zarrouk+Se culture medium for 10 days, and the strain culture conditions are as follows: 25-30 DEG C, pH=9.5, and light intensity is 40 muE.m-2.s-1 ‑2 ·S ‑1 After reaching the plateau, the strains are respectively transferred to a Zarrouk-N, a Zarrouk-Fe and a Zarrouk-Mg culture medium for induction culture for 2 days, and after collecting the algal powder, selenoprotein containing ergothioneine is obtained after purification. The application further screens a spirulina mutant strain with high selenium tolerance, wherein the algal strain tolerating 800 mg / L Na2SeO3 has the best salt tolerance and can tolerate 1000 mM NaCl; and it is found again through the nitrogen deficiency induction that the ergothioneine content can reach 103.65 mu g / g.

The present application relates to the technical field of biological medicine, and particularly relates to a method for extracting and determining selenoprotein from selenium-rich lycoperdon pygmaeum mycelium, wherein the lycoperdon pygmaeum mycelium fermented by a 20L biological culture tank is dried in a vacuum drying box, ground into standard powder through a 100-mesh sieve, 0.5g of the standard powder is weighed, a 0.05mol / L NaOH solution is added, ultrasonic-assisted extraction is performed, then centrifugation is performed at a speed of 3000r / min for 15min, the supernatant is adjusted to an isoelectric point, centrifugation is performed again to obtain a protein precipitate, and finally the protein precipitate is dried in a freeze dryer to obtain a protein sample. The present application is dedicated to using the selenium-rich lycoperdon pygmaeum mycelium as raw material, adopting ultrasonic extraction, optimally optimizing the extraction process of selenoprotein, quantitatively analyzing the selenium content in the protein, detecting the change of the type and quantity of amino acids before and after selenium enrichment through a thin layer chromatography test, and understanding the influence of selenium on the amino acids in the lycoperdon pygmaeum mycelium.

This invention discloses a selenylamide-based hydrogenation reaction of acetylacetamide compounds and its application in the modification of selenium-containing peptides and proteins, belonging to the fields of organic chemistry and chemical biology. The reaction involves the reaction of a selenium-containing compound with an acetylacetamide compound in a solvent with the addition of an additive. The applications of this reaction include: 1) selective modification and labeling of selenocysteine ​​in peptides and proteins; 2) cyclization reactions of selenium-containing peptides and selenoproteins; and 3) sequential modification of selenium-containing peptides in the presence of cysteine ​​by precise pH control combined with the hydrogen sulfide reaction of acetylacetamide. This reaction has advantages such as mild conditions, simple operation, fast reaction rate, high yield, good stability, and good selectivity. It provides a new and robust approach for the selective modification and labeling of selenium groups in bioconjugations, peptides, and / or proteins, and also provides a new method for the construction of cyclic peptides, offering a powerful tool for the development of selenium-containing peptide drugs.

The invention discloses a method for separating inorganic selenium from a selenoprotein primary product. The technical problem of separating inorganic selenium from the existing selenoprotein primary product is solved, the selenoprotein is purer, the organic selenium content (99.99%) is higher, the biological activity and effectiveness of selenium are improved, absorption and utilization of life bodies are facilitated, and the side effects of the selenium are reduced. Based on the characteristics that inorganic selenium is larger than organic selenium in density and higher in electric conductivity, the technical scheme of combining a precipitation method, an ultrafiltration method, a reverse osmosis method and a dialysis method is adopted, the inorganic selenium existing in selenoprotein is separated out, and a purer selenoprotein product is obtained. After selenium protein powder, selenium yeast powder, selenium mushroom powder, selenium algae powder and other organic selenium primary products are separated through the process steps, high-purity (95%-99.99%) selenium protein, inorganic selenium and purified water are obtained. Environmental protection requirements are considered preferentially, zero emission is achieved in the separation process, and the method is extremely environment-friendly. The selenium protein is pure, selenium supplementation is not limited any more, and selenium supplementation popularization becomes possible.

The invention provides an MCM-41 composite cinnamyl aldehyde-carvacrol essential oil nano edible film and a preparation method thereof. The preparation method comprises the following steps: preparing modified MCM-41, constructing MCM-41 composite cinnamyl aldehyde-carvacrol essential oil nano particles, and preparing a multifunctional composite edible film formula. According to the invention, cinnamyl aldehyde-carvacrol essential oil is wrapped with lecithin and selenoprotein, and MCM-41 is subjected to carboxyl modification. The modified MCM-41 can improve the compatibility with hydrophilic phospholipid and protein, and increase the loading capacity for essential oil nanoparticles. The loaded MCM-41 is compounded with gelatin, dextran and D-selenomethionine to form a film, and the D-selenomethionine has oxidation resistance, so that the edible film is endowed with excellent antibacterial and oxidation resistance, and the problems of single performance, poor stability of active ingredients and the like of a traditional edible film are solved.

The present invention provides a culture medium for cultivating Agaricus blazei Murr. rich in selenium amino acids and β-glucan, Agaricus blazei Murr., and its preparation method and application. The raw materials for preparing the culture medium for cultivating Agaricus blazei Murr. rich in selenium amino acids and β-glucan include the following components in parts by mass: Cardamine violifolia 4 - 12 parts; β-glucan 2 - 8 parts; corncob 6 - 14 parts; corn straw 15 - 25 parts; reed 12 - 18 parts; bamboo chips 12 - 18 parts; cow dung 15 - 25 parts; sheep dung or rapeseed cake 12 - 18 parts; rice bran 2 - 10 parts; lime 0.5 - 5 parts; superphosphate 0.5 - 5 parts. The selenium amino acid content in the fruiting bodies of Agaricus blazei Murr. cultivated with this culture medium is as high as 160 - 214 mg / kg, and the organic selenium content is higher than 90%. The active ingredient complex extracted from this Agaricus blazei Murr. contains rich nutrients such as selenium amino acids, selenocysteine, selenomethionine, selenoprotein, β-glucan, various vitamins, and trace elements.

The invention relates to the technical field of eye protection liquid, and discloses a preparation method of a vetch extract and eye protection liquid containing the extract. Traditional extraction and biological fermentation are combined, and a new component selenium element is given to the extract; through the whole series of processes, the final eye protection liquid product contains negative bivalent selenium protein components. The problems that the vetch extraction process is complex in technology, purification is carried out in the refining link, 'selenoprotein 'does not need to be researched independently, bioavailability is improved, and the factors that eye protection liquid is not clear, not enough in transparency, poor in atomization effect, large in irritation, poor in couperose skin removal capacity, good in stability and the like are solved. The obtained eye protection liquid has the advantages of being clear, high in transparency, good in atomization effect, small in irritation, high in couperose skin removing capacity, stable in property and the like.

The invention discloses a plant selenoprotein extracting solution graded filtering device and a filtering method.The plant selenoprotein extracting solution graded filtering device comprises an outer tank body of a cylindrical structure, a control panel is fixedly installed on the outer surface of the front side of the outer tank body, a feeding pipe is fixedly installed at the upper end of the outer tank body, and a discharging pipe and a blow-off pipe are fixedly installed on the lower surface of the feeding pipe; and a first filtering barrel of an uncovered structure is fixedly installed at the upper end of the interior of the outer tank body, a first filtering net is fixedly installed on the lower surface of the first filtering barrel, and a negative pressure head of an annular structure is fixedly installed on the lower surface of the first filtering barrel. According to the plant selenoprotein extracting solution graded filtering device and filtering method, a first filtering net on the lower surface of a first filtering barrel is used for primarily filtering an extracting solution, then a second filtering net in a second filtering barrel is used for secondarily filtering the extracting solution, a negative pressure head is started in the process, and air below the first filtering barrel is extracted under the action of the negative pressure head; the air pressure at the second filtering barrel is reduced, and bubbles in the extracting solution are quickly discharged.

The invention discloses a coprophilous fungus capsule freeze-drying protective agent for intestinal flora transplantation and a preparation method of the coprophilous fungus capsule freeze-drying protective agent for intestinal flora transplantation and belongs to the technical field related to pharmaceutical preparations, and the coprophilous fungus capsule freeze-drying protective agent for intestinal flora transplantation comprises nano-liposome; polydextrose; plant selenoprotein; tea polyphenol; vitamin E; chitosan oligosaccharide; an Omega-3 fatty acid; 3-5% of inulin; the nano-liposome forms a phospholipid bilayer to form a physical barrier on the surface of a cell, and polydextrose and inulin regulate the extracellular medium viscosity and inhibit the growth rate of ice crystals; omega-3 fatty acid is inserted into a phospholipid bilayer to maintain the fluidity of a membrane at a low temperature, vitamin E and tea polyphenol reduce membrane lipid peroxidation products in the freeze-drying process, and the stability of an oxidant cell membrane is protected through damage of ice crystals, so that cells are protected, and the survival of the cells is improved.

The invention relates to the technical field of plant selenium protein extraction, in particular to application of purple sweet potato selenium-rich protein in preparation of nutritional functional foods.The preparation method of the purple sweet potato selenium-rich protein comprises the following steps that selenium-rich purple sweet potatoes are subjected to vacuum freeze drying and then smashed; mixing the crushed sample with distilled water according to a mass-volume ratio of 1: (7-9), and simultaneously performing ultrasonic treatment; centrifuging, collecting supernate, adding ammonium sulfate into the supernate until the saturation reaches 50-70%, centrifuging, and collecting precipitate; dissolving the precipitate with distilled water, performing dialysis treatment in a dialysis bag, and performing freeze drying on a dialysis treatment product to obtain a purple sweet potato selenium-rich protein crude extract; the purple sweet potato selenium-rich protein is obtained after purification, and the nutritional functional product is a preparation with anti-inflammatory and anti-oxidation functions. The prepared purple sweet potato protein can play an anti-inflammatory role, a selenium-rich product shows a stronger function, and mechanism research and theoretical support are provided for application of the purple sweet potato selenium-rich protein in preparation of functional products.

The application discloses a plant selenoprotein enzyme peptide composition, effervescent tablets and application thereof, and relates to the field of functional food, and comprises the following components in parts by weight: selenium-rich shepherd's purse powder 5-12 parts; comprehensive fruit and vegetable fermentation powder 8-15 parts; soybean peptide powder 3-10 parts; comprehensive digestive enzyme 05-3 parts; and pharmaceutically or dietetically acceptable excipients; the plant selenoprotein enzyme peptide composition, the effervescent tablets and the application thereof are scientifically combined with organic selenium (selenium-rich shepherd's purse powder), active enzymes and small-molecule metabolites generated by fermentation of various fruits and vegetables, soybean peptides easy to absorb and prebiotics. Selenium is the core of the antioxidant system, peptides and fermentation products provide nutritional substrates and regulatory signals, prebiotics improve the intestinal microenvironment, and multiple components synergistically act on multiple pathways such as immunity and metabolism.

The invention belongs to the technical field of biological detection, and particularly relates to a non-invasive selenium level prediction method based on saliva selenoprotein P detection and application. According to the invention, a heterologous expression antigen SELENOP (40-194aa) is used for immunizing a mouse to generate an antibody, and the monoclonal antibody capable of efficiently recognizing SELENOP is obtained through screening; and establishing an ELISA detection method by using the obtained monoclonal antibody. By utilizing the detection method and an ICP-MS selenium content detection method, the selenoprotein P content in human plasma and saliva and the total selenium content in the plasma are detected and analyzed, and obvious positive correlation exists among the selenoprotein P content, the saliva selenoprotein P content and the total selenium content in the plasma, so that a correlation curve between the selenoprotein P in the saliva and the selenium content in the human plasma is established; and thus, the aim of non-invasively detecting and evaluating the selenium level of the human body through the saliva selenoprotein P is fulfilled. The technical scheme provided by the invention can be used for large-scale screening and conventional health monitoring.

The invention provides a method for extracting enriched selenium from selenium-enriched crops, which comprises the following steps: saccharifying steamed selenium-enriched crops with aspergillus tuberosus and trypsin, fermenting and distilling to obtain a selenium-enriched product. Trypsin degrades macromolecular protein in selenium-rich rice, saccharification and long-time fermentation enzymolysis stages promote selenoprotein to further hydrolyze volatile micromolecular selenium compounds, and finally the micromolecular selenium compounds and alcohol are distilled out through distillation and left in the distilled-out alcohol, so that the content of selenium in the alcohol is increased, the content of selenium in the selenium-rich rice is increased, and the content of selenium in the selenium-rich rice is increased. Selenium extraction and enrichment are realized; meanwhile, the flavor of the rice wine is also improved, so that the flavor is mellower, the fragrance is strong, and the taste is fresh and sweet.

The application discloses a kind of pectinatocarpus selenium protein peptide and albumin peptide formula and preparation method, the core active ingredient of the formula is the selenium protein peptide of pectinatocarpus source and egg white albumin peptide, wherein the number average molecular weight of selenium protein peptide is 461Da, total selenium is 412.67mg / kg and selenocysteine and pectinatocarpus plant selenium peptide ratio is more than 90%, and the biological potency of albumin peptide is 94, and the molecular weight is 200-800Da;The application promotes active transport in intestinal tract by albumin peptide as carrier, and is prepared by combining dry granulation and tabletting process, the selenium absorption rate of the application is greatly improved, realizes the synergistic effect of hypoglycemic, hypolipidemic and antioxidant, and provides a new scheme for selenium supplement functional food.

The invention provides a multi-selenocysteine selenoprotein W organic selenium preparation, and belongs to the technical field of biology. The preparation method comprises the following steps: constructing recombinant pichia pastoris for expressing the multi-selenocysteine selenoprotein W, fermenting, culturing and expressing the multi-selenocysteine selenoprotein W, and carrying out spray drying on fermented bacteria liquid to prepare the multi-selenocysteine selenoprotein W organic selenium preparation. The selenium content of a yeast strain for expressing the multi-selenocysteine selenoprotein W is 2.98 times of that of yeast for expressing natural selenoprotein W, and is 3.89 times of that of wild pichia pastoris. The polyselenocysteine selenoprotein W organic selenium preparation prepared through fermentation of recombinant pichia pastoris is high in organic selenium content and easy to absorb after being fed, the selenium content in blood of the white feather broilers can be effectively increased, the feed conversion ratio of the white feather broilers can be reduced, and the growth performance of the white feather broilers can be improved.

The present application belongs to the technical field of biological detection, and particularly relates to a non-invasive selenium level prediction method based on saliva selenoprotein P detection and application. The present application immunizes mice with heterologously expressed antigen SELENOP (40-194 aa) to generate antibodies, and monoclonal antibodies capable of efficiently recognizing SELENOP are screened and obtained; an ELISA detection method is established using the obtained monoclonal antibodies. The selenium content in human plasma and saliva is detected and analyzed using the detection method and an ICP-MS selenium content detection method, and it is found that there is a significant positive correlation between the three, and a correlation curve between saliva selenoprotein P and human plasma selenium content is established, thereby achieving the purpose of evaluating human selenium level through non-invasive saliva selenoprotein P detection. The technical scheme provided by the present application can be used for large-scale screening and routine health monitoring.

The invention discloses a nano-selenium foliar fertilizer and application thereof in efficient production of plant selenoprotein, the nano-selenium foliar fertilizer can reduce sodium selenite into elemental selenium by adding vitamin C and taking acid-soluble chitosan as a template, so that the content of selenium in the selenium is increased, and the content of selenium in the selenium is increased. The chance that selenium replaces sulfur in methionine and the chance that selenium is combined with other organic components in azolla filliculoidas are increased, and the selenium content of the azolla filliculoidas is greatly increased; the spreadability and adhesiveness of the selenium solution on plant leaves are improved by matching with the surfactant, the selenium solution can be better absorbed by the rumex hanus, cell protoplast flow is promoted by compounding with the plant growth regulator, the cell viability and the selenium utilization rate of plants are improved, and the growth and development of the plants are accelerated.

The invention discloses a synthesis method for synthesizing selenoprotein and secondarily synthesizing anthocyanin (glycoside) by using a spectrum of plant radish seeds, and relates to the technical field of trace element synthesis. The method comprises the following steps: step 1, selecting a plant radish seed with high absorptivity as a plant carrier, and cultivating in a plant factory; 2, supplementing light to the plants by adopting a composite spectrum, magnetizing water molecules from macromolecular groups into micromolecular groups by adopting a magnetization technology, carrying out sprinkling irrigation on the plants by matching with a nutritional agent, synthesizing the plant organic selenium into plant selenoprotein through plant absorption and a spectrum technology, and generating various trace elements; and step 3, in the plant cultivation process, synchronous secondary synthesis of anthocyanin is carried out through a spectrum synthesis technology. The production is simple and convenient, the cost is low, a special spectrum technology is adopted, light is supplemented to plants, organic selenium is synthesized into selenoprotein, multiple trace elements such as anthocyanin (glycoside) are synchronously and secondarily synthesized, and human body absorption is facilitated.

The invention relates to the field of preparation of pharmaceutical compositions, in particular to a preparation method of glycyrrhiza polysaccharide. The preparation method sequentially comprises the following steps: a solid state fermentation process, an alkali solution extraction process, a degreasing treatment process, a deproteinization treatment process, a decolorization treatment process, an ethanol graded extraction process, an amination modification process and a selenylation treatment process. The glycyrrhiza polysaccharide connected with selenoprotein obtained by the preparation method provided by the invention has the effects of resisting oxidation, improving immunity, resisting tumors and the like.

The invention discloses a preparation method of silkworm selenium-rich eggs, and belongs to the technical field of functional egg production. The preparation method of the silkworm selenium-rich eggs provided by the invention comprises the four steps of preparation of nano-selenium mulberry leaves, selenium-rich breeding of silkworms, preparation of silkworm selenoprotein and feeding management of laying hens. According to the method disclosed by the invention, the defects of low selenium utilization rate, high cost, serious pollution and the like in the traditional technology are overcome through collaborative optimization of a biological chain, efficient conversion and directional enrichment of selenium resources are realized, the selenium content of the obtained eggs is stabilized at 36.8 + / -1.9 mu g / egg, the proportion of selenomethionine is as high as 87.9%, the nutritional value and safety of the eggs are remarkably improved, and the method is suitable for industrial production. The method is suitable for industrial production.

The present invention discloses the application of selenoprotein P in diagnosing or predicting moyamoya disease, and specifically provides the application of a reagent for detecting selenoprotein P in preparing a product for diagnosing or predicting moyamoya disease. The present invention provides a product, system, and device for diagnosing or predicting moyamoya disease. The present invention first discovered that the expression level of selenoprotein P in the plasma of patients with moyamoya disease is reduced. By detecting selenoprotein P, it is possible to diagnose whether a subject suffers from moyamoya disease or has a risk of suffering from moyamoya disease, and has broad market application prospects.

The utility model relates to the technical field of cultivation devices, in particular to a selenium-containing protein carotenoid microalgae cultivation device. Aiming at the problem that the growth of microalgae is limited due to the change of external illumination and temperature and the inappropriate change of the concentration of carbon dioxide, so that the content of selenoprotein carotenoid is reduced, the following technical scheme is provided: the device comprises a box body, the top of the box body is rotatably connected with a box cover, and a cultivation groove is formed in the box body; a cultivation tank is placed in the cultivation groove, and microalgae are contained in the cultivation tank; the constant-temperature heating unit is mounted in the box body, and the top of the constant-temperature heating unit is in contact with a cultivation tank; the lamp strip is mounted on the inner wall of the cultivation tank and surrounds the outer ring of the cultivation tank. The device can automatically adjust the illumination, the temperature and the carbon dioxide content of microalgae breeding, improves the growth efficiency of microalgae, and ensures the growth of microalgae, the accumulation of nutritional ingredients and the final harvesting efficiency.