57 results about "Selenoprotein" patented technology

The invention discloses a bacterial selenoprotein online resource platform, an application method, a terminal and a medium, and relates to the technical field of biological medicine, the online resource platform is deployed on a server, the cloud server is a Ubuntu cloud server, and Nginx, Waitpress and Flask are configured; the server side is in butt joint with a background resource, and the background resource is in butt joint with the constructed bacterial selenoprotein database; the server is in butt joint with a user interface of the front end, the user interface designs a corresponding interface framework and an interaction function based on static resources hosted by Nginx, and the interaction function is used for realizing query, analysis and use of relevant information of the bacterial selenoprotein. According to the method, a convenient online access channel of an integrated database and a real-time data analysis tool are provided for users in related fields, and important support is provided for accurate annotation of selenoprotein genes in a bacterial genome plan.

The invention discloses a selenium-rich microecological preparation. The selenium-rich microecological preparation is prepared by the following steps: S1, activating strains; s2, preparing a selenomethionine premixed solution; s3, co-culture of bacteria and selenium; s4, forming a selenoprotein complex; s5, preparing a coating material; s6, coating with an emulsion; s7, acid-resistant layer coating; and S8, enteric activation treatment. According to different growth stages of the meat geese in the antibiotic-free breeding, a daily ration formula is directionally intervened: in the brooding period of the meat geese, the selenium-rich microecological preparation and daily ration feed are mixed in a dry mode, and conventional feeding is carried out; in the growth period of the meat geese, acidifying the selenium-rich microecological preparation, preparing the selenium-rich microecological preparation into a solution, and feeding the meat geese with the solution in the form of drinking water; in the fattening period of the meat geese, the premix containing the selenium-enriched microecological preparation is mixed with daily ration, and the meat geese are fed in an intensified mode. According to the invention, selenium-rich breeding of meat geese can be realized, economic benefits are increased, antibiotics can be replaced, and the eating safety and edible value of meat geese are improved.

The invention discloses a plant lactobacillus and application thereof, and belongs to the technical field of microorganisms. The plant lactobacillus 002P provided by the invention is a new strain, and is round, white, neat in edge, smooth in surface and soft in texture on an MRS solid culture medium; growth of bacteria can be remarkably inhibited, an inhibition effect on staphylococcus aureus, pseudomonas aeruginosa and escherichia coli is achieved, and the inhibition zone is 23.33-279.33 mm; the healing of infected wounds can be obviously promoted; the selenium conversion rate is excellent, and the maximum selenium conversion rate can reach 88%; the nano-selenium prepared from the plant lactobacillus 002P is spherical, has the particle size of 200-500nm, has excellent properties of resisting oxidation, relieving hepatotoxicity induced by saturated fatty acid, promoting expression of selenoprotein in a mouse body and reducing blood fat, and has a wide application prospect.

The invention relates to the field of edible mushroom product production equipment, in particular to an edible mushroom selenoprotein extraction device and method.The edible mushroom selenoprotein extraction device comprises a tank body, a tank bottom and a bottom pipe, the tank bottom is arranged at the bottom of the tank body, the tank bottom and the tank body form a cylinder structure, one side of the tank bottom inclines downwards, and the bottom pipe is communicated with the downward side of the tank bottom; the outer side of the bottom pipe is communicated with a material guiding pipe, a discharging pump is arranged between the material guiding pipe and the bottom pipe, and the outer side of the material guiding pipe is communicated with a material conveying barrel; and the rear end part of the material conveying barrel extends upwards in an inclined manner. Through a positioning wheel, a torsional spring and a rotating lug hinge structure of the stirring rod, the stirring rod is elastically attached to the inclined tank bottom and swings in a self-adaptive mode, an arc-shaped swing piece is matched to drive materials to turn over up and down, deposition dead angles are eliminated, contact between disintegrating slag and an extracting agent is promoted, and selenium protein dissolution uniformity is improved; staggered holes of the fixed spiral and the movable spiral form a spiral filtering channel, and when waste residues are conveyed in a rotating mode, extrusion percolation is conducted synchronously to achieve residual liquid recovery.

The application relates to a method for extracting ergothioneine and selenoprotein from spirulina, wherein after culturing FACHB-Se1500 strains under the above-mentioned nitrogen deficiency induction condition, a product is obtained, and after purification, selenoprotein containing ergothioneine is obtained; the FACHB-Se1500 strains are cultured in a Zarrouk+Se culture medium for 10 days, and the strain culture conditions are as follows: 25-30 DEG C, pH=9.5, and light intensity is 40 muE.m-2.s-1 ‑2 ·S ‑1 After reaching the plateau, the strains are respectively transferred to a Zarrouk-N, a Zarrouk-Fe and a Zarrouk-Mg culture medium for induction culture for 2 days, and after collecting the algal powder, selenoprotein containing ergothioneine is obtained after purification. The application further screens a spirulina mutant strain with high selenium tolerance, wherein the algal strain tolerating 800 mg / L Na2SeO3 has the best salt tolerance and can tolerate 1000 mM NaCl; and it is found again through the nitrogen deficiency induction that the ergothioneine content can reach 103.65 mu g / g.

The present application relates to the technical field of biological medicine, and particularly relates to a method for extracting and determining selenoprotein from selenium-rich lycoperdon pygmaeum mycelium, wherein the lycoperdon pygmaeum mycelium fermented by a 20L biological culture tank is dried in a vacuum drying box, ground into standard powder through a 100-mesh sieve, 0.5g of the standard powder is weighed, a 0.05mol / L NaOH solution is added, ultrasonic-assisted extraction is performed, then centrifugation is performed at a speed of 3000r / min for 15min, the supernatant is adjusted to an isoelectric point, centrifugation is performed again to obtain a protein precipitate, and finally the protein precipitate is dried in a freeze dryer to obtain a protein sample. The present application is dedicated to using the selenium-rich lycoperdon pygmaeum mycelium as raw material, adopting ultrasonic extraction, optimally optimizing the extraction process of selenoprotein, quantitatively analyzing the selenium content in the protein, detecting the change of the type and quantity of amino acids before and after selenium enrichment through a thin layer chromatography test, and understanding the influence of selenium on the amino acids in the lycoperdon pygmaeum mycelium.

This invention discloses a selenylamide-based hydrogenation reaction of acetylacetamide compounds and its application in the modification of selenium-containing peptides and proteins, belonging to the fields of organic chemistry and chemical biology. The reaction involves the reaction of a selenium-containing compound with an acetylacetamide compound in a solvent with the addition of an additive. The applications of this reaction include: 1) selective modification and labeling of selenocysteine ​​in peptides and proteins; 2) cyclization reactions of selenium-containing peptides and selenoproteins; and 3) sequential modification of selenium-containing peptides in the presence of cysteine ​​by precise pH control combined with the hydrogen sulfide reaction of acetylacetamide. This reaction has advantages such as mild conditions, simple operation, fast reaction rate, high yield, good stability, and good selectivity. It provides a new and robust approach for the selective modification and labeling of selenium groups in bioconjugations, peptides, and / or proteins, and also provides a new method for the construction of cyclic peptides, offering a powerful tool for the development of selenium-containing peptide drugs.

The invention discloses a method for separating inorganic selenium from a selenoprotein primary product. The technical problem of separating inorganic selenium from the existing selenoprotein primary product is solved, the selenoprotein is purer, the organic selenium content (99.99%) is higher, the biological activity and effectiveness of selenium are improved, absorption and utilization of life bodies are facilitated, and the side effects of the selenium are reduced. Based on the characteristics that inorganic selenium is larger than organic selenium in density and higher in electric conductivity, the technical scheme of combining a precipitation method, an ultrafiltration method, a reverse osmosis method and a dialysis method is adopted, the inorganic selenium existing in selenoprotein is separated out, and a purer selenoprotein product is obtained. After selenium protein powder, selenium yeast powder, selenium mushroom powder, selenium algae powder and other organic selenium primary products are separated through the process steps, high-purity (95%-99.99%) selenium protein, inorganic selenium and purified water are obtained. Environmental protection requirements are considered preferentially, zero emission is achieved in the separation process, and the method is extremely environment-friendly. The selenium protein is pure, selenium supplementation is not limited any more, and selenium supplementation popularization becomes possible.

The invention provides an MCM-41 composite cinnamyl aldehyde-carvacrol essential oil nano edible film and a preparation method thereof. The preparation method comprises the following steps: preparing modified MCM-41, constructing MCM-41 composite cinnamyl aldehyde-carvacrol essential oil nano particles, and preparing a multifunctional composite edible film formula. According to the invention, cinnamyl aldehyde-carvacrol essential oil is wrapped with lecithin and selenoprotein, and MCM-41 is subjected to carboxyl modification. The modified MCM-41 can improve the compatibility with hydrophilic phospholipid and protein, and increase the loading capacity for essential oil nanoparticles. The loaded MCM-41 is compounded with gelatin, dextran and D-selenomethionine to form a film, and the D-selenomethionine has oxidation resistance, so that the edible film is endowed with excellent antibacterial and oxidation resistance, and the problems of single performance, poor stability of active ingredients and the like of a traditional edible film are solved.

The present invention provides a culture medium for cultivating Agaricus blazei Murr. rich in selenium amino acids and β-glucan, Agaricus blazei Murr., and its preparation method and application. The raw materials for preparing the culture medium for cultivating Agaricus blazei Murr. rich in selenium amino acids and β-glucan include the following components in parts by mass: Cardamine violifolia 4 - 12 parts; β-glucan 2 - 8 parts; corncob 6 - 14 parts; corn straw 15 - 25 parts; reed 12 - 18 parts; bamboo chips 12 - 18 parts; cow dung 15 - 25 parts; sheep dung or rapeseed cake 12 - 18 parts; rice bran 2 - 10 parts; lime 0.5 - 5 parts; superphosphate 0.5 - 5 parts. The selenium amino acid content in the fruiting bodies of Agaricus blazei Murr. cultivated with this culture medium is as high as 160 - 214 mg / kg, and the organic selenium content is higher than 90%. The active ingredient complex extracted from this Agaricus blazei Murr. contains rich nutrients such as selenium amino acids, selenocysteine, selenomethionine, selenoprotein, β-glucan, various vitamins, and trace elements.

The invention discloses a plant selenoprotein extracting solution graded filtering device and a filtering method.The plant selenoprotein extracting solution graded filtering device comprises an outer tank body of a cylindrical structure, a control panel is fixedly installed on the outer surface of the front side of the outer tank body, a feeding pipe is fixedly installed at the upper end of the outer tank body, and a discharging pipe and a blow-off pipe are fixedly installed on the lower surface of the feeding pipe; and a first filtering barrel of an uncovered structure is fixedly installed at the upper end of the interior of the outer tank body, a first filtering net is fixedly installed on the lower surface of the first filtering barrel, and a negative pressure head of an annular structure is fixedly installed on the lower surface of the first filtering barrel. According to the plant selenoprotein extracting solution graded filtering device and filtering method, a first filtering net on the lower surface of a first filtering barrel is used for primarily filtering an extracting solution, then a second filtering net in a second filtering barrel is used for secondarily filtering the extracting solution, a negative pressure head is started in the process, and air below the first filtering barrel is extracted under the action of the negative pressure head; the air pressure at the second filtering barrel is reduced, and bubbles in the extracting solution are quickly discharged.

The invention discloses a coprophilous fungus capsule freeze-drying protective agent for intestinal flora transplantation and a preparation method of the coprophilous fungus capsule freeze-drying protective agent for intestinal flora transplantation and belongs to the technical field related to pharmaceutical preparations, and the coprophilous fungus capsule freeze-drying protective agent for intestinal flora transplantation comprises nano-liposome; polydextrose; plant selenoprotein; tea polyphenol; vitamin E; chitosan oligosaccharide; an Omega-3 fatty acid; 3-5% of inulin; the nano-liposome forms a phospholipid bilayer to form a physical barrier on the surface of a cell, and polydextrose and inulin regulate the extracellular medium viscosity and inhibit the growth rate of ice crystals; omega-3 fatty acid is inserted into a phospholipid bilayer to maintain the fluidity of a membrane at a low temperature, vitamin E and tea polyphenol reduce membrane lipid peroxidation products in the freeze-drying process, and the stability of an oxidant cell membrane is protected through damage of ice crystals, so that cells are protected, and the survival of the cells is improved.

The invention relates to the technical field of plant selenium protein extraction, in particular to application of purple sweet potato selenium-rich protein in preparation of nutritional functional foods.The preparation method of the purple sweet potato selenium-rich protein comprises the following steps that selenium-rich purple sweet potatoes are subjected to vacuum freeze drying and then smashed; mixing the crushed sample with distilled water according to a mass-volume ratio of 1: (7-9), and simultaneously performing ultrasonic treatment; centrifuging, collecting supernate, adding ammonium sulfate into the supernate until the saturation reaches 50-70%, centrifuging, and collecting precipitate; dissolving the precipitate with distilled water, performing dialysis treatment in a dialysis bag, and performing freeze drying on a dialysis treatment product to obtain a purple sweet potato selenium-rich protein crude extract; the purple sweet potato selenium-rich protein is obtained after purification, and the nutritional functional product is a preparation with anti-inflammatory and anti-oxidation functions. The prepared purple sweet potato protein can play an anti-inflammatory role, a selenium-rich product shows a stronger function, and mechanism research and theoretical support are provided for application of the purple sweet potato selenium-rich protein in preparation of functional products.

The application discloses a plant selenoprotein enzyme peptide composition, effervescent tablets and application thereof, and relates to the field of functional food, and comprises the following components in parts by weight: selenium-rich shepherd's purse powder 5-12 parts; comprehensive fruit and vegetable fermentation powder 8-15 parts; soybean peptide powder 3-10 parts; comprehensive digestive enzyme 05-3 parts; and pharmaceutically or dietetically acceptable excipients; the plant selenoprotein enzyme peptide composition, the effervescent tablets and the application thereof are scientifically combined with organic selenium (selenium-rich shepherd's purse powder), active enzymes and small-molecule metabolites generated by fermentation of various fruits and vegetables, soybean peptides easy to absorb and prebiotics. Selenium is the core of the antioxidant system, peptides and fermentation products provide nutritional substrates and regulatory signals, prebiotics improve the intestinal microenvironment, and multiple components synergistically act on multiple pathways such as immunity and metabolism.

The invention belongs to the technical field of biological detection, and particularly relates to a non-invasive selenium level prediction method based on saliva selenoprotein P detection and application. According to the invention, a heterologous expression antigen SELENOP (40-194aa) is used for immunizing a mouse to generate an antibody, and the monoclonal antibody capable of efficiently recognizing SELENOP is obtained through screening; and establishing an ELISA detection method by using the obtained monoclonal antibody. By utilizing the detection method and an ICP-MS selenium content detection method, the selenoprotein P content in human plasma and saliva and the total selenium content in the plasma are detected and analyzed, and obvious positive correlation exists among the selenoprotein P content, the saliva selenoprotein P content and the total selenium content in the plasma, so that a correlation curve between the selenoprotein P in the saliva and the selenium content in the human plasma is established; and thus, the aim of non-invasively detecting and evaluating the selenium level of the human body through the saliva selenoprotein P is fulfilled. The technical scheme provided by the invention can be used for large-scale screening and conventional health monitoring.

The application discloses a kind of pectinatocarpus selenium protein peptide and albumin peptide formula and preparation method, the core active ingredient of the formula is the selenium protein peptide of pectinatocarpus source and egg white albumin peptide, wherein the number average molecular weight of selenium protein peptide is 461Da, total selenium is 412.67mg / kg and selenocysteine and pectinatocarpus plant selenium peptide ratio is more than 90%, and the biological potency of albumin peptide is 94, and the molecular weight is 200-800Da;The application promotes active transport in intestinal tract by albumin peptide as carrier, and is prepared by combining dry granulation and tabletting process, the selenium absorption rate of the application is greatly improved, realizes the synergistic effect of hypoglycemic, hypolipidemic and antioxidant, and provides a new scheme for selenium supplement functional food.

The present application belongs to the technical field of biological detection, and particularly relates to a non-invasive selenium level prediction method based on saliva selenoprotein P detection and application. The present application immunizes mice with heterologously expressed antigen SELENOP (40-194 aa) to generate antibodies, and monoclonal antibodies capable of efficiently recognizing SELENOP are screened and obtained; an ELISA detection method is established using the obtained monoclonal antibodies. The selenium content in human plasma and saliva is detected and analyzed using the detection method and an ICP-MS selenium content detection method, and it is found that there is a significant positive correlation between the three, and a correlation curve between saliva selenoprotein P and human plasma selenium content is established, thereby achieving the purpose of evaluating human selenium level through non-invasive saliva selenoprotein P detection. The technical scheme provided by the present application can be used for large-scale screening and routine health monitoring.

The invention discloses a nano-selenium foliar fertilizer and application thereof in efficient production of plant selenoprotein, the nano-selenium foliar fertilizer can reduce sodium selenite into elemental selenium by adding vitamin C and taking acid-soluble chitosan as a template, so that the content of selenium in the selenium is increased, and the content of selenium in the selenium is increased. The chance that selenium replaces sulfur in methionine and the chance that selenium is combined with other organic components in azolla filliculoidas are increased, and the selenium content of the azolla filliculoidas is greatly increased; the spreadability and adhesiveness of the selenium solution on plant leaves are improved by matching with the surfactant, the selenium solution can be better absorbed by the rumex hanus, cell protoplast flow is promoted by compounding with the plant growth regulator, the cell viability and the selenium utilization rate of plants are improved, and the growth and development of the plants are accelerated.

The invention relates to the field of preparation of pharmaceutical compositions, in particular to a preparation method of glycyrrhiza polysaccharide. The preparation method sequentially comprises the following steps: a solid state fermentation process, an alkali solution extraction process, a degreasing treatment process, a deproteinization treatment process, a decolorization treatment process, an ethanol graded extraction process, an amination modification process and a selenylation treatment process. The glycyrrhiza polysaccharide connected with selenoprotein obtained by the preparation method provided by the invention has the effects of resisting oxidation, improving immunity, resisting tumors and the like.

The invention discloses a preparation method of silkworm selenium-rich eggs, and belongs to the technical field of functional egg production. The preparation method of the silkworm selenium-rich eggs provided by the invention comprises the four steps of preparation of nano-selenium mulberry leaves, selenium-rich breeding of silkworms, preparation of silkworm selenoprotein and feeding management of laying hens. According to the method disclosed by the invention, the defects of low selenium utilization rate, high cost, serious pollution and the like in the traditional technology are overcome through collaborative optimization of a biological chain, efficient conversion and directional enrichment of selenium resources are realized, the selenium content of the obtained eggs is stabilized at 36.8 + / -1.9 mu g / egg, the proportion of selenomethionine is as high as 87.9%, the nutritional value and safety of the eggs are remarkably improved, and the method is suitable for industrial production. The method is suitable for industrial production.

The utility model relates to the technical field of cultivation devices, in particular to a selenium-containing protein carotenoid microalgae cultivation device. Aiming at the problem that the growth of microalgae is limited due to the change of external illumination and temperature and the inappropriate change of the concentration of carbon dioxide, so that the content of selenoprotein carotenoid is reduced, the following technical scheme is provided: the device comprises a box body, the top of the box body is rotatably connected with a box cover, and a cultivation groove is formed in the box body; a cultivation tank is placed in the cultivation groove, and microalgae are contained in the cultivation tank; the constant-temperature heating unit is mounted in the box body, and the top of the constant-temperature heating unit is in contact with a cultivation tank; the lamp strip is mounted on the inner wall of the cultivation tank and surrounds the outer ring of the cultivation tank. The device can automatically adjust the illumination, the temperature and the carbon dioxide content of microalgae breeding, improves the growth efficiency of microalgae, and ensures the growth of microalgae, the accumulation of nutritional ingredients and the final harvesting efficiency.

The invention discloses a microbial selenium-rich polypeptide protein feed, and relates to the technical field of selenium-rich protein feeds. The feed specifically comprises the following components in percentage by mass: 5-20% of selenium-rich polypeptide, 5-20% of protein, 5-20% of trace elements, 5-20% of vitamins, 5-20% of dietary fibers, 5-20% of metabolites and 5-20% of an antioxidant. 30%-50% of protein; 0.5%-5% of trace elements; 0.1%-5% of vitamins; 8%-25% of dietary fibers; 3%-10% of metabolite; and 0.5%-5% of an antioxidant. The feed additive can effectively enhance the immunity of animals, promote growth and digestion and improve the meat quality and the product quality, is rich in selenium peptide, selenomethionine, glutathione and other components, has a remarkable anti-oxidation effect, can relieve oxidative stress and protect cell health, can effectively improve the feed conversion rate of the animals, and improves the feed utilization rate of the animals. Therefore, healthy growth and economic benefits are promoted.

The invention discloses a compound feed for cage-rearing laying hens to produce selenium-rich eggs as well as a preparation method and application of the compound feed. The compound feed comprises the following components in percentage by weight: 50%-65% of corn, 10%-20% of soybean meal, 5%-15% of rumex hanus powder, 3%-10% of fish meal, 1%-5% of a selenoprotein nutrient solution, 2%-8% of glucose, 1%-3% of zeolite powder, 5%-9% of stone powder, 1%-1.5% of calcium hydrophosphate, 0.2%-0.4% of table salt and 0.5%-1% of a laying hen premix. The preparation method comprises the steps of raw material crushing, mixing, selenoprotein nutrient solution spraying, final mixing and the like. The method is characterized in that through the synergistic effect of the rumex hanus powder, the selenoprotein nutrient solution, the glucose and the zeolite powder, the deposition efficiency and stability of selenium in the eggs are remarkably improved. The feed is fed to laying hens (for example, the laying hens are 300 days old) in the egg producing period, selenium-rich eggs with the selenium content reaching 0.42 mg / Kg can be stably produced, the national standard is met, and the feed is high in safety and easy to popularize.

The invention relates to the technical field of extraction of microbial functional components, in particular to selenium polypeptide with high antioxidant activity as well as a preparation method and application of the selenium polypeptide. The selenium polypeptide provided by the invention is a selenium polypeptide substance with more prominent yeast source high biological activity compared with selenium-enriched yeast, the ABTS free radical scavenging rate reaches 65.51-80.23%, the antioxidant activity under the isoconcentration condition is 5.24-6.42 times of that of total selenium polypeptide, 11.99-14.69 times of that of selenium-enriched protein and 11.18-13.69 times of that of the selenium-enriched yeast, the selenium content is 3646.96-4919.69 mg / kg, and the content of selenium in the selenium-enriched yeast is more than that of the total selenium polypeptide. Compared with selenium-enriched yeast, the selenium-enriched yeast has the advantages of high activity and high absorption efficiency, and has a wide application prospect in the aspects of medicine, functional food, cosmetics and the like.

This invention discloses a serine tRNA synthetase mutant and its applications, belonging to the field of enzyme proteins. This invention utilizes machine learning techniques such as AlphaFold3 to predict SerRS and tRNA... Sec The interaction interface, thereby precisely locating the SerRS that affects tRNA Sec By identifying key amino acid residues and employing a semi-rational mutation strategy, SerRS key sites were targeted and modified to enhance their binding to tRNA. Sec By combining binding affinity and catalytic efficiency, a high tRNA binding affinity and catalytic efficiency were constructed. Sec The SerRS variant with aminoacylation activity improves the supply level of Sec, enabling efficient and high-fidelity expression of selenoproteins, eliminating the mis-incorporation of Ser, and providing a new technical means for the biosynthesis of selenoproteins.

The invention discloses a process for producing enzyme selenoprotein by liquid compound fermentation of compound strains, and relates to the technical field of biological fermentation, enterococcus faecalis, aspergillus niger and bacillus mucilaginosus are adopted to obtain a mixed strain according to a specific proportion, the mixed strain is added into a breeding culture medium, sodium selenite is added after amplification, and the enzyme selenoprotein is obtained. Sodium selenite is absorbed by mixed strains and converted into enzyme selenoprotein, repeated fermentation is adopted, the mixed strains and sodium selenite are supplemented, and along with increase of the number of times of fermentation, the metabolic capacity of the strains is gradually enhanced, the content of metabolites is increased, and the content of selenoprotein is gradually increased and tends to be stable; according to the present invention, the stopping of the fermentation process can be timely determined according to the requirement on the product enzyme selenoprotein, after the repeated fermentation, the molecular weight of the enzyme selenoprotein is from 3000 Dalton to 18000 Dalton, the fermentation frequency can be selected according to the requirement, the larger the molecular weight of the enzyme selenoprotein is, the better the absorption is, and the wide application prospect is provided.

The invention belongs to the technical field of medical biological detection, and provides application of selenoprotein P as a target spot in tumor diagnosis and treatment. The invention specifically provides application of a reagent for detecting the expression level of selenoprotein P in preparation of a tumor diagnosis kit, provides a corresponding detection kit, and further provides application of the reagent as a target spot in preparation of tumor treatment drugs and a corresponding pharmaceutical composition. The invention discloses a key function of the Selenop in interaction of the RTMs and the tumor cells, and the key function is specifically shown as that the Selenop is transferred from the RTMs to the tumor cells, lipid oxidation is inhibited, and the tumor cells are protected from ferroptosis. Based on the discovery, the invention provides an application prospect of Selenop as a tumor treatment target, and provides a potential strategy and method for tumor immunotherapy.

The present application relates to a high selenium-tolerant tree tongue mutant strain and its application, belonging to the technical field of fungal breeding. It discloses a high selenium-tolerant and selenium-rich tree tongue mutant strain YL108, which has been preserved in China Center for Type Culture Collection (CCTCC) in Wuhan University, Wuhan, China, classified as Ganoderma applanatum YL108, with the preservation number CCTCC NO: M 20221347 and the preservation date of August 29, 2022. The strain can maintain normal growth in a culture medium with a selenium content of 90 mg / L, and the mycelium obtained has no red selenium phenomenon, and the amino acid form selenium content in the mycelium protein is up to 511 mg / kg, accounting for about 55% of the total protein selenium. The above-mentioned high selenium-tolerant tree tongue mutant strain Ganoderma applanatum YL108 is more suitable for cultivation in a high-selenium-content medium, and high-quality selenium-containing protein with high amino acid form selenium is obtained, which is a high-quality germplasm resource for preparing fungal selenium-rich products.

The present invention relates to the field of biomedicine technology, and in particular to a method for preparing a thyroid immunomodulatory drug based on a selenoprotein-modified astragalus polysaccharide derivative. The method comprises the following steps: in a first reactor, adding astragalus polysaccharide and maleic anhydride to a Tris-HCl buffer for reaction to obtain olefinated astragalus polysaccharide; in a second reactor, adding food-grade selenoprotein and 2-iminothiolane hydrochloride to a Tris-HCl buffer for reaction to obtain thiolated selenoprotein; pouring the second reactor into the first reactor, adding cysteine ​​to obtain a selenoprotein-modified astragalus polysaccharide derivative; mixing the selenoprotein-modified astragalus polysaccharide derivative, starch, and magnesium stearate, and pressing to obtain the thyroid immunomodulatory drug; the thyroid immunomodulatory drug prepared by the present invention has a positive effect on the immunoregulation of thyroid FRTL-5 cells.

This invention discloses the application of selenoprotein M in the preparation of drugs for treating abdominal aortic aneurysms. This invention also provides the application of selenoprotein M in the preparation of drugs for reducing macrophage inflammation. This invention can effectively delay the progression of abdominal aortic aneurysms and reduce macrophage inflammation, and has broad prospects for medical applications.