178 results about "Selenoprotein" patented technology

The invention discloses a selenium-enriched rice planting method. The method includes the following steps of land selecting, field finishing, seed treatment, sowing and seedling raising, seedling transplant, fertilizer management in the later period, irrigation treatment and selenium-enriched rice harvesting. Before seedlings are transplanted, selenium-enriched base fertilizer in a complex state is applied in the rice field, and a certain selenium source is provided for rice; at the beginning of panicle emergence and flowering of rice and grouting, the selenium-enriched compound fertilizer is applied in the rice field again, the selenium-enriched fertilizer is evenly distributed around roots of the seedlings in the growth process, the selenium in a complex state from soil is absorbed by the roots of the plants and then enters the inner cycle systems of the plants and participates in synthesis of protein through the path from stems to leaves to flowers and fruits, and finally, the selenium in an ionic condition from soil is stored in the seeds in the form of selenoprotein. The selenium-enriched rice tastes good and is high in nutrient matter content, and the content of the selenium of the rice can reach over 0.2 mg/kg and meets the national selenium-enriched standard.

The invention relates to an extraction method of cardamine hupingshanesis plant selenoprotein. The extraction method comprises the following steps: (1) cardamine hupingshanesis is degreased; (2) the cardamine hupingshanesis is dipped by 75% of an alcohol solution at the temperature of 60 minus 5 to 60 plus 5 DEG C; (3) a mixture obtained after dipping is filtered, so as to remove the alcohol solution, and powder is obtained by drying; (4) the obtained powder is added with water, the water extraction operation is performed at the temperature of 60-80 DEG C; water extraction liquid is filtered and concentrated to obtain concentrated liquid; (5) ethanol is added in the concentrated liquid, the volume of the added ethanol is 70% of the total volume of the solution, and an alcohol precipitate is obtained through still standing or filtering; (6) the alcohol precipitate is added with water, and then is added with pectinase, heat preservation is performed for 40 minus 5 to 40 plus 5 minutes, thermostable amylase is added, and heat preservation is performed for 30 minus 5 to 30 plus 5 minutes, so that hydrolysate is obtained; (7) the hydrolysate is added with ethanol, the volume of the added ethanol is more than 75% of the total volume of the solution, and precipitates are obtained through still standing or filtering; (8) the precipitates are dried. The extraction method is suitable for extracting the cardamine hupingshanesis plant selenoprotein in large scale.

The invention relates to the field of selenium-rich rice byproducts, and in particular relates to a preparation method of rice bran selenoprotein powder. The method comprises the following steps of: grinding, screening and degreasing byproduct rice bran obtained after rice processing to prepare rough rice bran into degreased rice bran, mixing 60%-70% alcohol with the degreased rice bran in the material-liquid ratio of (1:3)-(1:5), stirring on a magnetic force mixing machine for a certain period of time, and centrifuging, wherein the processes are repeated for three times; and carrying out alkaline extraction with 0.02-0.1mol/L NaOH solution for 1-3 hours in the material-liquid ratio of (1:10)-(1:20) at the temperature of 30-40 DEG C, taking supernate after centrifuging, decolorizing with active carbon, adjusting an isoelectric point by utilizing a acetic acid-natrium aceticum buffer solution for acid deposition, then centrifuging, and freezing to dry after washing, thereby obtaining the rice bran selenoprotein powder with the protein extraction ratio of 60-80%, and the selenium content of 0.2-0.3mg/kg finally. The selenoprotein powder provided by the invention is natural in nutritional ingredient, is rich in trace element selenium, keeps the original nutrition ingredients in the rice bran selenoprotein powder effectively, and is suitably used as a food auxiliary material or a nutrition enhancer.

The invention relates to a method for preparing an organic selenium fertilizer through hydrolysis of selenium-enriched yeast. The method comprises the following steps: with selenium-enriched yeast as a raw material, allowing selenium-enriched yeast cells to undergone wall breaking and selenoprotein to undergone hydrolysis under acidic and thermal conditions so as to obtain selenoamino acid and oligopeptides thereof; carrying out cooling to room temperature; and adjusting the pH value of hydrolysate to allow the hydrolysate to be neutral, thereby obtaining the micromolecular organic selenium fertilizer. The method has the advantages of easiness, convenient operation and easy realization of industrial production. The prepared organic selenium fertilizer has the main components--selenoamino acid and oligopeptides thereof, has a small molecular weight and can be easily absorbed by plants. When the micromolecular organic selenium fertilizer is applied to crops, the contents of organic selenium in fruit and seeds are greatly increased, the outputs of the crops are increased, the added values of agricultural products are enhanced, and economic benefits of the crops are improved.

The invention discloses a method for extraction of selenoprotein from selenium-rich tea leaves. The method includes: taking selenium-rich tea leaves as the raw materials, subjecting the selenium-rich tea leaves to drying, crushing and sieving, adding ethanol, conducting heating in water bath at 60DEG C for 30min, performing centrifugation to obtain precipitate, repeating the steps, and then carrying out drying, crushing and sieving; adding distilled water into the obtained precipitate, conducting freezing for 3-5h in a low temperature refrigerator, conducting heating in water bath at 50-70DEG C for 30min, then performing freezing in the low temperature refrigerator for 3-5h, conducting heating in water bath at 50-70DEG C for 30min, and repeating the operation three times; adding a NaOH solution, conducting heating in water bath at 70-90DEG C for 3-4h, performing centrifugation under 3500rpm for 10min, repeating the process again on the precipitate, adjusting the pH of the supernatant to 2.5-3.5, carrying out standing, centrifugation and drying to obtain coarse selenoprotein. The method provided by the invention has the advantages of simple preparation process, easy operation and environmental friendliness. The obtained product has high selenoprotein content, and is a reliable source of organic selenium. Thus, the method is suitable for industrial production.

The invention discloses a method for extracting selenoprotein from selenium-rich rice bran. The method comprises the following steps of performing ultrasonic extraction on fresh selenium-rich rice bran powder and a prepared extraction solution in an ultrasonic washing machine; then performing decoloring and performing centrifugation for precipitation; adjusting isoelectric points with citric acid, and performing centrifugation for collecting precipitate; removing impurities with a dialysis bag; and finally, performing freeze drying so as to obtain the selenoprotein. According to the method disclosed by the invention, a manner of reducing extraction and performing acid precipitation improved, so that selenoprotein is obtained, and the protein extraction rate is 80% or above; and the extracted selenoprotein is few in impurities, the protein content is as high as 80-95%, and the selenium content of the selenoprotein is as high as 0.50-0.58mg/kg. The method is simple and convenient, conditions are mild, the production technology is simple, industrial popularization is easy and the method has high utilization value.

The invention discloses a method for synchronously extracting proanthocyanidin and selenium-enriched protein from black peanut skin. Firstly, the black peanut skin subjected to liquid nitrogen freezing superfine grinding, then ultrasonic-assisted extraction of the proanthocyanidin and ultrasonic-assisted extraction of the selenium-enriched protein are performed simultaneously, prepared proanthocyanidin powder is high in proanthocyanidin content and high in activity, and the selenium-enriched protein is high in organic selenium content and high in activity. With the adoption of the method for preparing two active substances (the proanthocyanidin and the selenium-enriched protein) from the raw material, namely, the black peanut skin, the technological process can be simplified, the raw material cost can be reduced, the working efficiency can be improved, in addition, the method is high in extraction rate and suitable for industrial production, and the activity of extracted substances is high.

The invention relates to a preparation method of selenium-enriched fruit and vegetable essence tablet with adjuvant cancer treatment effect. The selenium-enriched fruit and vegetable essence tablets with adjuvant cancer treatment effects are prepared from the following raw materials in percentage by weight: 0.01-0.03% of plant selenoprotein, 5-10% of selenium-enriched garlic extract, 5-10% of selenium-enriched broccolis, 1-3% of selenium-enriched soybeans, 5-10% of selenium-enriched malt extract, 3-10% of selenium-enriched green tea, 3-10% of sweet potato extract, 3-10% of wheat bran extract, 3-10% of cucumber extract, 3-10% of asparagus extract, 3-10% of kelp extract, 3-10% of coix seed extract, 3-15% of mushroom extract, 3-15% of cordyceps extract, 3-15% of refined Poria cocos powder, 3-15% of Grossularia burejensis Berger. extract and 3-15% of B-glucan. No antiseptic is added into the fruit and vegetable essence tablets; the fruit and vegetable essence tablets are extracted from pure natural plants, and are capable of reinforcing human immunity, as well as preventing and treating cancer as an adjuvant therapy; moreover, the fruit and vegetable essence tablets are free of side effects and adverse reactions., and can be conveniently taken. Modern preparation technology and advanced quality control method are utilized in the preparation method, so that the fruit and vegetable essence tablets are safe, stable and controllable in quality.

The present invention relates to a pure natural plant selenoprotein preparation method. The pure natural plant selenoprotein recipe is as follows: 300g-500g of selenium enriched cardamine hupingshanensis, 200g-400g of selenium enriched broccoli, 50g-150g of selenium enriched radix astragali, 50g-150g of selenium enriched garlics and 50g-150g of selenium enriched soybeans. The plant selenoprotein extraction method can extract water soluble plant selenoproteins. Through ninhydrin assay, the result shows that the proportion of the plant selenoproteins can reach about 98%. The organic selenium content of the prepared products can reach 99%, and the pure natural plant selenoprotein is high in safety, good in water solubility, and high in human body absorption and utilization rates.

The invention discloses an extraction device and technology of selenium polypeptide from soybeans. The extraction technology comprises the steps of step (1) preparing materials; step (2) extracting selenium protein powder; step (3) performing pulp mixing; step (4) performing ultrasonic enzymolysis; step (5) performing modification; step (6) removing impurities; and step (7) performing concentration and drying. According to the extraction device and technology, the selenium protein is extracted firstly, then the emulsion is subjected to enzymolysis twice and modified, the extracted selenium polypeptide is low in the number of impurities, the purity is high, the selenium polypeptide is free from peculiar smell, and the technical problem that in the prior art, many extraction processes are used, inconvenient to use and low in efficacy can be effectively solved.

The invention discloses a method for extracting plant selenoprotein from selenium-rich rice. The method comprises steps as follows: (1), pretreatment; (2), extraction treatment: slurry obtained after enzymolysis is mixed with an extract liquid, a mixture is subjected to far-infrared ray irradiation treatment as assistance, subjected to water bath oscillation for 1-6 h and centrifuged for 15-22 min at the temperature of 25 DEG C at the rotating speed of 3000-5000 r/min, and an obtained supernatant is a protein liquid; (3), deposition treatment: the protein liquid is enabled to be under the condition of ice bath, icy acetone 4 times the volume of the protein liquid is added, then a mixed liquid is put in an environment at the temperature of subzero 27 DEG C-subzero 17 DEG C and is placed for 3-5 h under the assistant treatment condition that sound energy density of ultrasonic waves is 10-18 W/mL for selenoprotein deposition, the mixed liquid is centrifuged for 10-15 min, precipitates are taken out and subjected to spray drying, and finally, finished selenoprotein products are obtained. The extracted plant selenoprotein has the advantages of small grain diameter and high extraction rate and the method has broad market popularization value.

The invention provides toxicity-removing composite tablets containing selenoprotein and glossy ganoderma components and a preparation method of the toxicity-removing composite tablets. The toxicity-removing composite tablets not only can complement selenium but also can strengthen glossy ganoderma, so that heavy metal toxicity can be relieved. The composite tablets are reasonable in preparation working procedures and high in maneuverability, feed proportioning is optimized, and selenium-enriched rice bran protein, glossy ganoderma polysaccharide and glossy ganoderma tritici are combined with an excipient required for tabletting, so that the tablets are made, and besides, the composite tablets which are rich in nutrition, comprehensive in functions, and capable of removing toxicity are made; and besides, an industry chain of deeply processing selenium-rich rice and glossy ganoderma can be extended.

The invention provides a selenoprotein chewable tablet and a preparation method thereof. The preparation method comprises the following steps: by taking Se-enriched tea albumen powder as a main raw material, beta-cyclodextrin as a bitter-removing agent, citric acid or malic acid or citric acid-malic acid as an acidulant, xylitol and lactitol as a sweetening agent and microcrystalline cellulose as a filler, crushing the main and auxiliary materials; sieving; then fully and uniformly mixing to obtain a mixed material; adding gelatin into the mixed material as a wetting agent; after preparing a soft material, primarily sieving and granulating; then drying again; carrying out secondary sieving, straightening granules and adding magnesium stearate; fully and uniformly mixing; and tabletting, carrying out ultraviolet sterilization and packaging to obtain the selenoprotein chewable tablet. The selenoprotein chewable tablet provided by the invention has the characteristics of simple preparation process, simplicity, convenience and feasibility in operation, environmental-friendliness, accurate dosage, convenience in carrying, stable quality, convenience in delivery and the like, is suitable for people to supplement selenium safely anytime and anywhere, and is suitable for industrial production.

The invention discloses a selenium-enriched rice plant selenoprotein capsule material. The selenium-enriched rice plant selenoprotein capsule material comprises the following raw materials in parts by weight: 10 to 20 parts of selenium-enriched rice plant selenoprotein, 15 to 20 parts of sodium alginate, 6 to 11 parts of water-soluble cellulose, 13 to 28 parts of carboxymethyl starch, 5 to 9 parts of collagen, 8 to 14 parts of okra extract, 3 to 5 parts of hyaluronic acid, 1 to 3 parts of glycerinum, 2 parts of vitamin E and 150 to 200 parts of deionized water. The invention also discloses a preparation method of the selenium-enriched rice plant selenoprotein capsule material. The raw materials can be completely dispersed and combined tightly, adsorption of a human body is benefited, and the capsule material has high mechanical property under the condition of low humidity, is favorable for storage and transportation of capsules, and has great market promotion value.

The invention discloses a preparation method of a high-flavone selenium-enriched ampelopsis grossedentata granular beverage. The preparation method comprises the following technological steps of extracting ampelopsis grossedentata flavone, performing freeze drying on ampelopsis grossedentata leaves, performing superfine comminution, adding inulin/selenoprotein, performing granulation, performing drying and the like. The ampelopsis grossedentata granular beverage made by the method not only has higher nutrient and health-care value but also has good sensory evaluation effects.

The present invention discloses a health-care quick-frozen boiled dumpling recipe. The health-care quick-frozen boiled dumpling recipe comprises the following components in ratios: boiled dumpling wrapper materials: wheat flour, buckwheat flour, vegetable protein powder, modified starch, carrageenan, walnut kernel powder, a stabilizer and a modifier; and boiled dumpling stuffing materials: vegetables, meat, spices, pigment, spices, steviol glycosides, dipotassium glycyrrhizinate, bioflavonoids, selenoproteins and vitamin E. The processing method includes material preparing, wrapper preparing, stuffing preparing, pressed sealing and forming, quick-freezing, sterilizing and packaging. The recipe is reasonable, the technology is scientific, and the prepared boiled dumplings are good in mouthfeel, unique in flavor, and rich in nutrition, benefit health, maximally retain nutrients, and have better health-care effects.

The invention provides an extraction method of selenoprotein in cardamine violifolia, the selenoprotein obtained by the extraction method and application of the selenoprotein. The extraction method comprises the following steps of carrying out enzymolysis on the cardamine violifolia by adopting enzymes including cellulase to obtain a cardamine violifolia enzymatic hydrolysate; and concentrating the cardamine violifolia enzymatic hydrolysate, and drying the concentrated cardamine violifolia enzymatic hydrolysate. According to the method disclosed by the invention, the selenoprotein in the cardamine violifolia is extracted through enzymolysis of the enzymes including the cellulase, so that nutritional ingredients in the cardamine violifolia are not damaged, the efficiency of extracting the selenoprotein through enzymolysis is high, the yield of the selenoprotein is relatively high, the protein content in the selenoprotein is high, the content of total selenium is high, and the total selenium is organic selenium; and in addition, the extraction method is simple, efficient, low in cost, environmentally friendly and easy for large-scale industrial production.

The invention discloses a method for extracting selenoprotein from selenium-rich edible fungi, relates to the field of selenoprotein extraction, and aims to solve the problem that natural edible fungiare quite low in selenium content and needs to be supplemented with the selenium content. The method specifically comprises the following steps: step one, preparing selenium-rich edible fungi; step two, acid extraction; step three, enzymolysis; step four, preparation of crude selenoprotein liquid; step five, preparation of a finished product: adding the crude selenoprotein liquid into an ethanolsolution of which the mass fraction is 88-94% and the volume is 3-5 times that of the crude selenoprotein liquid for alcoholysis to obtain precipitates, and sequentially washing the precipitates withabsolute ethyl alcohol, petroleum ether and acetone and carrying out vacuum drying to obtain the finished product. According to the invention, selenium is enriched in the edible fungi and converted into organic selenium, selenoprotein is obtained by means of enzymolysis, centrifugation, filtration and the like, the extraction rate of the selenoprotein is high, and the purity of the obtained selenoprotein is high. The finished product obtained in the invention can be applied to the field of food processing, meets demands of selenium supplementation of human bodies, and has wide application prospect.

The invention provides a selenium-rich oral solution with effects of reducing blood sugar, reducing blood fat and boosting immunity. The selenium-rich oral solution comprises the following raw materials: cyclocarya paliurus, Hubei malus spectabilis, radix polygonati officinalis, chaenomeles speciosa and selenoprotein. A preparation method for the selenium-rich oral solution comprises the following steps: 1) respectively smashing or mixing and then smashing cyclocarya paliurus, Hubei malus spectabilis, radix polygonati officinalis and chaenomeles speciosa, thereby acquiring four powdered raw materials or mixed raw material powder; 2) respectively mixing the four powdered raw materials with water, heating and refluxing, thereby acquiring four filtrates, or mixing the mixed raw material powder with water, heating and refluxing, thereby acquiring a mixed filtrate; 3) mixing the four filtrates or mixing the mixed filtrate with a flocculating agent, flocculating, heating, separating solid from liquid, and using the acquired liquid as a clear liquor; and 4) concentrating the clear liquor, adding selenoprotein, seasoning, sterilizing and filling, thereby acquiring the selenium-rich oral solution. The invention also provides an application of the selenium-rich oral solution in preparing food or medicines for reducing blood sugar, reducing blood fat and boosting immunity.

The invention discloses a special-dietary selenium-rich dried meat and a making method thereof. The dried meat is prepared from, by weight, 100-130 parts of livestock and poultry animal meats, 12-26 parts of selenoprotein and pickling materials, wherein the pickling materials are prepared from 75-90 parts of soybean sauce, 35-50 parts of sugar, 6-10 parts of baijiu, 0.5-1.5 parts of pepper, 0.5-1.5 parts of fennel, 0.5-1.5 parts of licorice roots, 1-3 parts of anise, 2-4 parts of cinnamon, 1-2 parts of cloves, 10-15 parts of orange peels and 3-7 parts of fresh ginger. The making method includes the steps of 1, raw material pretreating; 2, pickling material preparing; 3, pickling; 4, recombining; 5, blocking; 6, cooking; 7, particle cutting; 8, drying; 9, packaging and sterilizing. According to the special-dietary selenium-rich dried meat and the making method thereof, the nutritive value of the livestock and poultry animal meats and the plant selenoprotein is fully used, the selenium content in the produced dried meat reaches about 12 microgram/gram, and the requirement of the acceptable daily intake required by human-body nutrition is met; the making method is suitable for producing the special-dietary selenium-rich dried meat.

The invention relates to selenoprotein extracted from selenium-rich cereals and selenium-rich brassica juncea. The selenoprotein comprises the following components in percentage by weight: 50%-99.9% of selenium-rich cereal proteins and 0.1%-50% of selenium-rich brassica juncea proteins. The invention also provides a method for preparing the selenoprotein. The method comprises the following steps of: preparing the selenium-rich cereal proteins; preparing the selenium-rich brassica juncea proteins; mixing the selenium-rich cereal proteins with the selenium-rich brassica juncea proteins in proportion. According to the selenoprotein disclosed by the invention, selenium-rich proteins of rice husks and selenoprotein of the selenium-rich brassica juncea are compounded, so that selenium-rich ricehusk resources are effectively utilized, the effect of intensifying selenium elements is achieved, not only can the shortcoming of low selenomethionine of the selenoprotein of the selenium-rich brassica juncea be overcome, but also the defect of the selenium-rich proteins of rice husks in the aspect of the selenocysteine can be eliminated, the biological effects of various selenoproteins containing the selenomethionine and selenocysteine mutually cooperate, and the requirements of modern people for multiple aspects of functional health-care products are met.

The invention discloses a feed additive for improving the flavor of livestock meat and prolonging the shelf life of the livestock meat. The feed additive comprises the following main raw material components in part by weight: 1 to 5 parts of magnesium methionine, 4 to 8 parts of insect chitosan or/and selenoprotein, 1 to 5 parts of sodium glutamate, 1 to 5 parts of premix for use in livestock latter fattening period and 0.5 to 1 part of vitamin. The feed additive prepared by the invention can be added into livestock feed directly. When the livestock are fed by the feed containing the additive, the normal metabolic function of the digestive system, particularly the liver tissue, of the livestock can be maintained; the accumulation amount and position of the metabolic reactions of the fat, proteins, micro elements and the like can be regulated; and finally, the flavor of the livestock meat can be improved and the shelf life of the livestock meat can be prolonged. The raw materials of the invention are widely available and can be purchased in market easily; the preparation process is simple; and the feed additive is convenient to use and environmentally-friendly, and avoids any harmful residue.

The invention provides selenium-rich passion fruit tea and a preparation method thereof. The selenium-rich passion fruit tea is prepared from the following raw materials in parts by weight: 40-60 parts of passion fruit, 10-20 parts of jasmine flower, 80-100 parts of tea base, 5-10 parts of purple potato powder, 0.5-1 part of mustard, 1-5 parts of syrup, 2-8 parts of yeast, 10-15 parts of selenoprotein, 1-5 parts of Corydalis bungeana, 1-5 parts of Scutellaria barbata,1-5 parts of senna leaf, 0.1-1 part of non-dairy creamer, 1-5 parts of Pueraria lobata flower, 0.1-1 part of taichi stone, 1-5 parts of Homalomena occulta, and 1-5 parts of Hippocampus. The selenium-rich passion fruit tea provided by the invention has thick flavor and unique taste, and has the health effects of clearing away summer heat, refreshing mind, relieving fatigue, promoting appetite, promoting metabolism, smoothing gallbladder, protecting liver, improving eyesight, protecting eyes, inducing diuresis to reduce edema, and sending up the lucid yang. The preparation method is simple. The selenium-rich passion fruit tea is suitable for generalization in the field of drink.

The present invention relates to a health-care food containing selenoproteins. The health-care food containing the selenoproteins comprises the following components in parts: 20-25 parts of selenoproteins, 1-5 parts of starch, 1-3 parts of a flavoring agent, 1-5 parts of fruit powder and 10-15 parts of other auxiliary materials. The present invention also provides a preparation method of the health-care food containing the selenoproteins. The preparation method comprises mixing and granulating; and finally, the materials are prepared into medicines or health-care foods of different dosage forms. Rice bran selenium-enriched proteins and selenium-enriched brassica juncea selenoproteins are compounded to prepare the medicines or health-care foods of the different dosage forms, which effectively utilizes the selenium-enriched rice bran resource, also plays a function of strengthening selenium element, can solve a problem of relatively low selenomethionine in the brassica juncea selenoproteins, can also eliminate a deficiency of selenocysteine in the rice bran selenium-enriched proteins, thus can enable the biological effects of the various selenoproteins containing the selenomethionineand selenocysteine to be cooperated with each other, and meets needs of modern people for many aspects of functional health-care products.