20 results about "Selenoprotein" patented technology

The invention discloses a bacterial selenoprotein online resource platform, an application method, a terminal and a medium, and relates to the technical field of biological medicine, the online resource platform is deployed on a server, the cloud server is a Ubuntu cloud server, and Nginx, Waitpress and Flask are configured; the server side is in butt joint with a background resource, and the background resource is in butt joint with the constructed bacterial selenoprotein database; the server is in butt joint with a user interface of the front end, the user interface designs a corresponding interface framework and an interaction function based on static resources hosted by Nginx, and the interaction function is used for realizing query, analysis and use of relevant information of the bacterial selenoprotein. According to the method, a convenient online access channel of an integrated database and a real-time data analysis tool are provided for users in related fields, and important support is provided for accurate annotation of selenoprotein genes in a bacterial genome plan.

The application relates to a method for extracting ergothioneine and selenoprotein from spirulina, wherein after culturing FACHB-Se1500 strains under the above-mentioned nitrogen deficiency induction condition, a product is obtained, and after purification, selenoprotein containing ergothioneine is obtained; the FACHB-Se1500 strains are cultured in a Zarrouk+Se culture medium for 10 days, and the strain culture conditions are as follows: 25-30 DEG C, pH=9.5, and light intensity is 40 muE.m-2.s-1 ‑2 ·S ‑1 After reaching the plateau, the strains are respectively transferred to a Zarrouk-N, a Zarrouk-Fe and a Zarrouk-Mg culture medium for induction culture for 2 days, and after collecting the algal powder, selenoprotein containing ergothioneine is obtained after purification. The application further screens a spirulina mutant strain with high selenium tolerance, wherein the algal strain tolerating 800 mg / L Na2SeO3 has the best salt tolerance and can tolerate 1000 mM NaCl; and it is found again through the nitrogen deficiency induction that the ergothioneine content can reach 103.65 mu g / g.

The present application relates to the technical field of biological medicine, and particularly relates to a method for extracting and determining selenoprotein from selenium-rich lycoperdon pygmaeum mycelium, wherein the lycoperdon pygmaeum mycelium fermented by a 20L biological culture tank is dried in a vacuum drying box, ground into standard powder through a 100-mesh sieve, 0.5g of the standard powder is weighed, a 0.05mol / L NaOH solution is added, ultrasonic-assisted extraction is performed, then centrifugation is performed at a speed of 3000r / min for 15min, the supernatant is adjusted to an isoelectric point, centrifugation is performed again to obtain a protein precipitate, and finally the protein precipitate is dried in a freeze dryer to obtain a protein sample. The present application is dedicated to using the selenium-rich lycoperdon pygmaeum mycelium as raw material, adopting ultrasonic extraction, optimally optimizing the extraction process of selenoprotein, quantitatively analyzing the selenium content in the protein, detecting the change of the type and quantity of amino acids before and after selenium enrichment through a thin layer chromatography test, and understanding the influence of selenium on the amino acids in the lycoperdon pygmaeum mycelium.

This invention discloses a selenylamide-based hydrogenation reaction of acetylacetamide compounds and its application in the modification of selenium-containing peptides and proteins, belonging to the fields of organic chemistry and chemical biology. The reaction involves the reaction of a selenium-containing compound with an acetylacetamide compound in a solvent with the addition of an additive. The applications of this reaction include: 1) selective modification and labeling of selenocysteine ​​in peptides and proteins; 2) cyclization reactions of selenium-containing peptides and selenoproteins; and 3) sequential modification of selenium-containing peptides in the presence of cysteine ​​by precise pH control combined with the hydrogen sulfide reaction of acetylacetamide. This reaction has advantages such as mild conditions, simple operation, fast reaction rate, high yield, good stability, and good selectivity. It provides a new and robust approach for the selective modification and labeling of selenium groups in bioconjugations, peptides, and / or proteins, and also provides a new method for the construction of cyclic peptides, offering a powerful tool for the development of selenium-containing peptide drugs.

The invention discloses a method for separating inorganic selenium from a selenoprotein primary product. The technical problem of separating inorganic selenium from the existing selenoprotein primary product is solved, the selenoprotein is purer, the organic selenium content (99.99%) is higher, the biological activity and effectiveness of selenium are improved, absorption and utilization of life bodies are facilitated, and the side effects of the selenium are reduced. Based on the characteristics that inorganic selenium is larger than organic selenium in density and higher in electric conductivity, the technical scheme of combining a precipitation method, an ultrafiltration method, a reverse osmosis method and a dialysis method is adopted, the inorganic selenium existing in selenoprotein is separated out, and a purer selenoprotein product is obtained. After selenium protein powder, selenium yeast powder, selenium mushroom powder, selenium algae powder and other organic selenium primary products are separated through the process steps, high-purity (95%-99.99%) selenium protein, inorganic selenium and purified water are obtained. Environmental protection requirements are considered preferentially, zero emission is achieved in the separation process, and the method is extremely environment-friendly. The selenium protein is pure, selenium supplementation is not limited any more, and selenium supplementation popularization becomes possible.

The invention discloses a plant selenoprotein extracting solution graded filtering device and a filtering method.The plant selenoprotein extracting solution graded filtering device comprises an outer tank body of a cylindrical structure, a control panel is fixedly installed on the outer surface of the front side of the outer tank body, a feeding pipe is fixedly installed at the upper end of the outer tank body, and a discharging pipe and a blow-off pipe are fixedly installed on the lower surface of the feeding pipe; and a first filtering barrel of an uncovered structure is fixedly installed at the upper end of the interior of the outer tank body, a first filtering net is fixedly installed on the lower surface of the first filtering barrel, and a negative pressure head of an annular structure is fixedly installed on the lower surface of the first filtering barrel. According to the plant selenoprotein extracting solution graded filtering device and filtering method, a first filtering net on the lower surface of a first filtering barrel is used for primarily filtering an extracting solution, then a second filtering net in a second filtering barrel is used for secondarily filtering the extracting solution, a negative pressure head is started in the process, and air below the first filtering barrel is extracted under the action of the negative pressure head; the air pressure at the second filtering barrel is reduced, and bubbles in the extracting solution are quickly discharged.

The invention discloses a coprophilous fungus capsule freeze-drying protective agent for intestinal flora transplantation and a preparation method of the coprophilous fungus capsule freeze-drying protective agent for intestinal flora transplantation and belongs to the technical field related to pharmaceutical preparations, and the coprophilous fungus capsule freeze-drying protective agent for intestinal flora transplantation comprises nano-liposome; polydextrose; plant selenoprotein; tea polyphenol; vitamin E; chitosan oligosaccharide; an Omega-3 fatty acid; 3-5% of inulin; the nano-liposome forms a phospholipid bilayer to form a physical barrier on the surface of a cell, and polydextrose and inulin regulate the extracellular medium viscosity and inhibit the growth rate of ice crystals; omega-3 fatty acid is inserted into a phospholipid bilayer to maintain the fluidity of a membrane at a low temperature, vitamin E and tea polyphenol reduce membrane lipid peroxidation products in the freeze-drying process, and the stability of an oxidant cell membrane is protected through damage of ice crystals, so that cells are protected, and the survival of the cells is improved.

The application discloses a plant selenoprotein enzyme peptide composition, effervescent tablets and application thereof, and relates to the field of functional food, and comprises the following components in parts by weight: selenium-rich shepherd's purse powder 5-12 parts; comprehensive fruit and vegetable fermentation powder 8-15 parts; soybean peptide powder 3-10 parts; comprehensive digestive enzyme 05-3 parts; and pharmaceutically or dietetically acceptable excipients; the plant selenoprotein enzyme peptide composition, the effervescent tablets and the application thereof are scientifically combined with organic selenium (selenium-rich shepherd's purse powder), active enzymes and small-molecule metabolites generated by fermentation of various fruits and vegetables, soybean peptides easy to absorb and prebiotics. Selenium is the core of the antioxidant system, peptides and fermentation products provide nutritional substrates and regulatory signals, prebiotics improve the intestinal microenvironment, and multiple components synergistically act on multiple pathways such as immunity and metabolism.

The application discloses a kind of pectinatocarpus selenium protein peptide and albumin peptide formula and preparation method, the core active ingredient of the formula is the selenium protein peptide of pectinatocarpus source and egg white albumin peptide, wherein the number average molecular weight of selenium protein peptide is 461Da, total selenium is 412.67mg / kg and selenocysteine and pectinatocarpus plant selenium peptide ratio is more than 90%, and the biological potency of albumin peptide is 94, and the molecular weight is 200-800Da;The application promotes active transport in intestinal tract by albumin peptide as carrier, and is prepared by combining dry granulation and tabletting process, the selenium absorption rate of the application is greatly improved, realizes the synergistic effect of hypoglycemic, hypolipidemic and antioxidant, and provides a new scheme for selenium supplement functional food.

The present application belongs to the technical field of biological detection, and particularly relates to a non-invasive selenium level prediction method based on saliva selenoprotein P detection and application. The present application immunizes mice with heterologously expressed antigen SELENOP (40-194 aa) to generate antibodies, and monoclonal antibodies capable of efficiently recognizing SELENOP are screened and obtained; an ELISA detection method is established using the obtained monoclonal antibodies. The selenium content in human plasma and saliva is detected and analyzed using the detection method and an ICP-MS selenium content detection method, and it is found that there is a significant positive correlation between the three, and a correlation curve between saliva selenoprotein P and human plasma selenium content is established, thereby achieving the purpose of evaluating human selenium level through non-invasive saliva selenoprotein P detection. The technical scheme provided by the present application can be used for large-scale screening and routine health monitoring.

The invention discloses a compound feed for cage-rearing laying hens to produce selenium-rich eggs as well as a preparation method and application of the compound feed. The compound feed comprises the following components in percentage by weight: 50%-65% of corn, 10%-20% of soybean meal, 5%-15% of rumex hanus powder, 3%-10% of fish meal, 1%-5% of a selenoprotein nutrient solution, 2%-8% of glucose, 1%-3% of zeolite powder, 5%-9% of stone powder, 1%-1.5% of calcium hydrophosphate, 0.2%-0.4% of table salt and 0.5%-1% of a laying hen premix. The preparation method comprises the steps of raw material crushing, mixing, selenoprotein nutrient solution spraying, final mixing and the like. The method is characterized in that through the synergistic effect of the rumex hanus powder, the selenoprotein nutrient solution, the glucose and the zeolite powder, the deposition efficiency and stability of selenium in the eggs are remarkably improved. The feed is fed to laying hens (for example, the laying hens are 300 days old) in the egg producing period, selenium-rich eggs with the selenium content reaching 0.42 mg / Kg can be stably produced, the national standard is met, and the feed is high in safety and easy to popularize.

The invention relates to the technical field of extraction of microbial functional components, in particular to selenium polypeptide with high antioxidant activity as well as a preparation method and application of the selenium polypeptide. The selenium polypeptide provided by the invention is a selenium polypeptide substance with more prominent yeast source high biological activity compared with selenium-enriched yeast, the ABTS free radical scavenging rate reaches 65.51-80.23%, the antioxidant activity under the isoconcentration condition is 5.24-6.42 times of that of total selenium polypeptide, 11.99-14.69 times of that of selenium-enriched protein and 11.18-13.69 times of that of the selenium-enriched yeast, the selenium content is 3646.96-4919.69 mg / kg, and the content of selenium in the selenium-enriched yeast is more than that of the total selenium polypeptide. Compared with selenium-enriched yeast, the selenium-enriched yeast has the advantages of high activity and high absorption efficiency, and has a wide application prospect in the aspects of medicine, functional food, cosmetics and the like.

The invention belongs to the technical field of medical biological detection, and provides application of selenoprotein P as a target spot in tumor diagnosis and treatment. The invention specifically provides application of a reagent for detecting the expression level of selenoprotein P in preparation of a tumor diagnosis kit, provides a corresponding detection kit, and further provides application of the reagent as a target spot in preparation of tumor treatment drugs and a corresponding pharmaceutical composition. The invention discloses a key function of the Selenop in interaction of the RTMs and the tumor cells, and the key function is specifically shown as that the Selenop is transferred from the RTMs to the tumor cells, lipid oxidation is inhibited, and the tumor cells are protected from ferroptosis. Based on the discovery, the invention provides an application prospect of Selenop as a tumor treatment target, and provides a potential strategy and method for tumor immunotherapy.

The present application relates to a high selenium-tolerant tree tongue mutant strain and its application, belonging to the technical field of fungal breeding. It discloses a high selenium-tolerant and selenium-rich tree tongue mutant strain YL108, which has been preserved in China Center for Type Culture Collection (CCTCC) in Wuhan University, Wuhan, China, classified as Ganoderma applanatum YL108, with the preservation number CCTCC NO: M 20221347 and the preservation date of August 29, 2022. The strain can maintain normal growth in a culture medium with a selenium content of 90 mg / L, and the mycelium obtained has no red selenium phenomenon, and the amino acid form selenium content in the mycelium protein is up to 511 mg / kg, accounting for about 55% of the total protein selenium. The above-mentioned high selenium-tolerant tree tongue mutant strain Ganoderma applanatum YL108 is more suitable for cultivation in a high-selenium-content medium, and high-quality selenium-containing protein with high amino acid form selenium is obtained, which is a high-quality germplasm resource for preparing fungal selenium-rich products.

This invention relates to the field of *Paenibacillus polymyxa*, addressing the problem of low production of immune signaling proteins in existing *Paenibacillus* strains. It provides a new *Paenibacillus polymyxa* strain, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC NO.36323. This invention provides a novel *Paenibacillus* strain that possesses both high production of immune signaling proteins and natural biocontrol and growth-promoting properties. It can secrete polymyxin-like antibacterial substances and simultaneously synthesize PR proteins and selenomethionine, overcoming the limitations of traditional *Paenibacillus* strains that only inhibit bacterial growth and lack immune signal transduction. The preparation method provided by this invention involves the strain converting sodium selenite into nano-selenium, which combines with the secreted PR protein to form nano-selenoprotein. Simultaneously, it synergistically produces polymyxins, achieving a dual effect of immune signal activation and direct bacterial inhibition, solving the problems of weak signaling and lack of synergistic antibacterial effects of traditional inducers.

The utility model relates to the technical field of food material selenoprotein extraction, in particular to equipment for extracting high-selenium food material selenoprotein, which comprises an extraction tank, a bottom shell is movably mounted at the bottom end of the extraction tank, and a sealing mechanism is arranged at the joint of the extraction tank and the bottom shell; a movable disc is movably mounted at the upper end of the interior of the extraction tank, and a driving mechanism is arranged between the movable disc and the upper end of the extraction tank. Through cooperative use of the sleeves, the piston rod, the spring, the movable disc and the corrugated pipe, the transmission shaft controls the cutting knife to cut up food materials falling at the upper end of the filtering disc, then clear water is pumped from the outside, enters the flow dividing pipe from the water inlet pipe and is divided into the two sleeves through the flow dividing pipe, and the clear water presses the piston rod downwards to cut up the food materials. The piston rod controls the movable disc to descend and stretches the spring, food material residues remaining on the inner wall of the extraction tank are scraped, filtered by the filtering disc and then discharged, in this way, lifting can be controlled through water pressure, and the equipment cost and the processing cost are reduced.

The invention discloses a three-selenium composition for promoting proliferation of beneficial bacteria and a preparation method thereof, the three-selenium composition is characterized in that the three-selenium composition is prepared from selenoprotein prepared from mulberry leaves, selenium-rich tea leaves and cardamine violifolia as raw materials, nano selenium-rich yeast and L-selenium-methyl selenocysteine, the components of the three-selenium composition are unique in preparation, the three-selenium composition can generate a synergistic interaction effect under a specific ratio, and the composition has a good effect on promoting proliferation of beneficial bacteria. The selenium-enriched yeast is an organic selenium component, the obtained product not only can safely supplement selenium, but also has the effects of promoting proliferation of beneficial bacteria and enhancing immunity, and the technical requirements of symbolic components, pesticide residues and heavy metal content of the product are ensured by adopting an ultrasonic enzymolysis and biotransformation method and a process of nanocrystallizing the selenium-enriched yeast and adsorbing and filtering an extracting solution. The product provided by the invention has the advantages of large market demand, wide market demand, wide application prospect, facilitation of promotion of popularization and application of precise selenium supplement, facilitation of promotion of development of agricultural product planting and processing industry, and substantial social and economic benefits.

The invention relates to the technical field of biological medicine, in particular to application of selenoprotein nanoparticles in preparation of medicine for preventing and treating diabetic macrovascular complications. Specifically, the selenoprotein nanoparticles are applied to preparation of drugs for preventing and treating diabetic macrovascular complications. The selenoprotein nano-particles prepared by the invention have excellent biocompatibility, good activity, stability, high efficiency and simple preparation process, can be used as a novel nano-protein drug for prevention and treatment of diabetic cardiovascular diseases, and have good application prospects.

The invention discloses application of selenoprotein O in lipid metabolism regulation and mitochondrial protection, and belongs to the technical field of biochemistry. The technical problem to be solved is that specific mechanisms of lipid metabolism regulation and mitochondrial stress protection in the prior art are not clear. According to the key point of the technical scheme, the application of selenoprotein O in lipid metabolism regulation and mitochondrial protection is provided, NAD < + > in mitochondria is hydrolyzed into NMN and AMP under the action of SelO, Mn < 2 + > is used as a cofactor, the reaction is sensitive to enhanced mitochondrial matrix pH reaction, the mitochondria is protected from continuous excessive metabolism activation, and in addition, the selenoprotein O can be used as a lipid metabolism regulator. The SelO directly interacts with fatty acid oxidation related enzymes to inhibit lipid catabolism. The discovery of the application reveals a conservative space-time NAD + regulation mechanism, and highlights the physiological importance of the space-time NAD + regulation mechanism in prokaryotes and eukaryotes; the kit or the medicine for adjusting the activity of the selenoprotein O in the mitochondria can be used for preparing products for preventing or treating mitochondria-related diseases or preparing weight-losing products.

The application belongs to the technical field of protein extraction, and particularly relates to an insect selenium-rich protein extraction and fermentation device and a use method thereof. The device comprises a mixed liquid tank, a water pump connected to a liquid outlet of the mixed liquid tank through a pipeline, a fermentation tank connected to a liquid outlet of the water pump through a pipeline, an ultrasonic cell disrupter connected to a liquid outlet of the fermentation tank through a pipeline, a lipid removal tank connected to a liquid outlet of the ultrasonic cell disrupter through a pipeline, a first centrifugal filter connected to a liquid outlet of the lipid removal tank through a pipeline, a rotor pump connected to a protein outlet of the first centrifugal filter through a pipeline, and an enzymolysis barrel connected to a liquid outlet of the rotor pump through a pipeline. In the application, each pitch of the helical blade is used as a hydrolysis chamber, the environment in the chamber of the enzymolysis barrel is stabilized, the hydrolysis environment of the protein is stabilized, and the degree of hydrolysis of the protein molecules in each independent chamber is stabilized after hydrolysis.