38 results about "Selenoprotein" patented technology

The invention discloses a bacterial selenoprotein online resource platform, an application method, a terminal and a medium, and relates to the technical field of biological medicine, the online resource platform is deployed on a server, the cloud server is a Ubuntu cloud server, and Nginx, Waitpress and Flask are configured; the server side is in butt joint with a background resource, and the background resource is in butt joint with the constructed bacterial selenoprotein database; the server is in butt joint with a user interface of the front end, the user interface designs a corresponding interface framework and an interaction function based on static resources hosted by Nginx, and the interaction function is used for realizing query, analysis and use of relevant information of the bacterial selenoprotein. According to the method, a convenient online access channel of an integrated database and a real-time data analysis tool are provided for users in related fields, and important support is provided for accurate annotation of selenoprotein genes in a bacterial genome plan.

The invention relates to the field of edible mushroom product production equipment, in particular to an edible mushroom selenoprotein extraction device and method.The edible mushroom selenoprotein extraction device comprises a tank body, a tank bottom and a bottom pipe, the tank bottom is arranged at the bottom of the tank body, the tank bottom and the tank body form a cylinder structure, one side of the tank bottom inclines downwards, and the bottom pipe is communicated with the downward side of the tank bottom; the outer side of the bottom pipe is communicated with a material guiding pipe, a discharging pump is arranged between the material guiding pipe and the bottom pipe, and the outer side of the material guiding pipe is communicated with a material conveying barrel; and the rear end part of the material conveying barrel extends upwards in an inclined manner. Through a positioning wheel, a torsional spring and a rotating lug hinge structure of the stirring rod, the stirring rod is elastically attached to the inclined tank bottom and swings in a self-adaptive mode, an arc-shaped swing piece is matched to drive materials to turn over up and down, deposition dead angles are eliminated, contact between disintegrating slag and an extracting agent is promoted, and selenium protein dissolution uniformity is improved; staggered holes of the fixed spiral and the movable spiral form a spiral filtering channel, and when waste residues are conveyed in a rotating mode, extrusion percolation is conducted synchronously to achieve residual liquid recovery.

The application relates to a method for extracting ergothioneine and selenoprotein from spirulina, wherein after culturing FACHB-Se1500 strains under the above-mentioned nitrogen deficiency induction condition, a product is obtained, and after purification, selenoprotein containing ergothioneine is obtained; the FACHB-Se1500 strains are cultured in a Zarrouk+Se culture medium for 10 days, and the strain culture conditions are as follows: 25-30 DEG C, pH=9.5, and light intensity is 40 muE.m-2.s-1 ‑2 ·S ‑1 After reaching the plateau, the strains are respectively transferred to a Zarrouk-N, a Zarrouk-Fe and a Zarrouk-Mg culture medium for induction culture for 2 days, and after collecting the algal powder, selenoprotein containing ergothioneine is obtained after purification. The application further screens a spirulina mutant strain with high selenium tolerance, wherein the algal strain tolerating 800 mg / L Na2SeO3 has the best salt tolerance and can tolerate 1000 mM NaCl; and it is found again through the nitrogen deficiency induction that the ergothioneine content can reach 103.65 mu g / g.

The present application relates to the technical field of biological medicine, and particularly relates to a method for extracting and determining selenoprotein from selenium-rich lycoperdon pygmaeum mycelium, wherein the lycoperdon pygmaeum mycelium fermented by a 20L biological culture tank is dried in a vacuum drying box, ground into standard powder through a 100-mesh sieve, 0.5g of the standard powder is weighed, a 0.05mol / L NaOH solution is added, ultrasonic-assisted extraction is performed, then centrifugation is performed at a speed of 3000r / min for 15min, the supernatant is adjusted to an isoelectric point, centrifugation is performed again to obtain a protein precipitate, and finally the protein precipitate is dried in a freeze dryer to obtain a protein sample. The present application is dedicated to using the selenium-rich lycoperdon pygmaeum mycelium as raw material, adopting ultrasonic extraction, optimally optimizing the extraction process of selenoprotein, quantitatively analyzing the selenium content in the protein, detecting the change of the type and quantity of amino acids before and after selenium enrichment through a thin layer chromatography test, and understanding the influence of selenium on the amino acids in the lycoperdon pygmaeum mycelium.

This invention discloses a selenylamide-based hydrogenation reaction of acetylacetamide compounds and its application in the modification of selenium-containing peptides and proteins, belonging to the fields of organic chemistry and chemical biology. The reaction involves the reaction of a selenium-containing compound with an acetylacetamide compound in a solvent with the addition of an additive. The applications of this reaction include: 1) selective modification and labeling of selenocysteine ​​in peptides and proteins; 2) cyclization reactions of selenium-containing peptides and selenoproteins; and 3) sequential modification of selenium-containing peptides in the presence of cysteine ​​by precise pH control combined with the hydrogen sulfide reaction of acetylacetamide. This reaction has advantages such as mild conditions, simple operation, fast reaction rate, high yield, good stability, and good selectivity. It provides a new and robust approach for the selective modification and labeling of selenium groups in bioconjugations, peptides, and / or proteins, and also provides a new method for the construction of cyclic peptides, offering a powerful tool for the development of selenium-containing peptide drugs.

The invention discloses a method for separating inorganic selenium from a selenoprotein primary product. The technical problem of separating inorganic selenium from the existing selenoprotein primary product is solved, the selenoprotein is purer, the organic selenium content (99.99%) is higher, the biological activity and effectiveness of selenium are improved, absorption and utilization of life bodies are facilitated, and the side effects of the selenium are reduced. Based on the characteristics that inorganic selenium is larger than organic selenium in density and higher in electric conductivity, the technical scheme of combining a precipitation method, an ultrafiltration method, a reverse osmosis method and a dialysis method is adopted, the inorganic selenium existing in selenoprotein is separated out, and a purer selenoprotein product is obtained. After selenium protein powder, selenium yeast powder, selenium mushroom powder, selenium algae powder and other organic selenium primary products are separated through the process steps, high-purity (95%-99.99%) selenium protein, inorganic selenium and purified water are obtained. Environmental protection requirements are considered preferentially, zero emission is achieved in the separation process, and the method is extremely environment-friendly. The selenium protein is pure, selenium supplementation is not limited any more, and selenium supplementation popularization becomes possible.

The invention provides an MCM-41 composite cinnamyl aldehyde-carvacrol essential oil nano edible film and a preparation method thereof. The preparation method comprises the following steps: preparing modified MCM-41, constructing MCM-41 composite cinnamyl aldehyde-carvacrol essential oil nano particles, and preparing a multifunctional composite edible film formula. According to the invention, cinnamyl aldehyde-carvacrol essential oil is wrapped with lecithin and selenoprotein, and MCM-41 is subjected to carboxyl modification. The modified MCM-41 can improve the compatibility with hydrophilic phospholipid and protein, and increase the loading capacity for essential oil nanoparticles. The loaded MCM-41 is compounded with gelatin, dextran and D-selenomethionine to form a film, and the D-selenomethionine has oxidation resistance, so that the edible film is endowed with excellent antibacterial and oxidation resistance, and the problems of single performance, poor stability of active ingredients and the like of a traditional edible film are solved.

The invention discloses a plant selenoprotein extracting solution graded filtering device and a filtering method.The plant selenoprotein extracting solution graded filtering device comprises an outer tank body of a cylindrical structure, a control panel is fixedly installed on the outer surface of the front side of the outer tank body, a feeding pipe is fixedly installed at the upper end of the outer tank body, and a discharging pipe and a blow-off pipe are fixedly installed on the lower surface of the feeding pipe; and a first filtering barrel of an uncovered structure is fixedly installed at the upper end of the interior of the outer tank body, a first filtering net is fixedly installed on the lower surface of the first filtering barrel, and a negative pressure head of an annular structure is fixedly installed on the lower surface of the first filtering barrel. According to the plant selenoprotein extracting solution graded filtering device and filtering method, a first filtering net on the lower surface of a first filtering barrel is used for primarily filtering an extracting solution, then a second filtering net in a second filtering barrel is used for secondarily filtering the extracting solution, a negative pressure head is started in the process, and air below the first filtering barrel is extracted under the action of the negative pressure head; the air pressure at the second filtering barrel is reduced, and bubbles in the extracting solution are quickly discharged.

The invention discloses a coprophilous fungus capsule freeze-drying protective agent for intestinal flora transplantation and a preparation method of the coprophilous fungus capsule freeze-drying protective agent for intestinal flora transplantation and belongs to the technical field related to pharmaceutical preparations, and the coprophilous fungus capsule freeze-drying protective agent for intestinal flora transplantation comprises nano-liposome; polydextrose; plant selenoprotein; tea polyphenol; vitamin E; chitosan oligosaccharide; an Omega-3 fatty acid; 3-5% of inulin; the nano-liposome forms a phospholipid bilayer to form a physical barrier on the surface of a cell, and polydextrose and inulin regulate the extracellular medium viscosity and inhibit the growth rate of ice crystals; omega-3 fatty acid is inserted into a phospholipid bilayer to maintain the fluidity of a membrane at a low temperature, vitamin E and tea polyphenol reduce membrane lipid peroxidation products in the freeze-drying process, and the stability of an oxidant cell membrane is protected through damage of ice crystals, so that cells are protected, and the survival of the cells is improved.

The invention relates to the technical field of plant selenium protein extraction, in particular to application of purple sweet potato selenium-rich protein in preparation of nutritional functional foods.The preparation method of the purple sweet potato selenium-rich protein comprises the following steps that selenium-rich purple sweet potatoes are subjected to vacuum freeze drying and then smashed; mixing the crushed sample with distilled water according to a mass-volume ratio of 1: (7-9), and simultaneously performing ultrasonic treatment; centrifuging, collecting supernate, adding ammonium sulfate into the supernate until the saturation reaches 50-70%, centrifuging, and collecting precipitate; dissolving the precipitate with distilled water, performing dialysis treatment in a dialysis bag, and performing freeze drying on a dialysis treatment product to obtain a purple sweet potato selenium-rich protein crude extract; the purple sweet potato selenium-rich protein is obtained after purification, and the nutritional functional product is a preparation with anti-inflammatory and anti-oxidation functions. The prepared purple sweet potato protein can play an anti-inflammatory role, a selenium-rich product shows a stronger function, and mechanism research and theoretical support are provided for application of the purple sweet potato selenium-rich protein in preparation of functional products.

The application discloses a plant selenoprotein enzyme peptide composition, effervescent tablets and application thereof, and relates to the field of functional food, and comprises the following components in parts by weight: selenium-rich shepherd's purse powder 5-12 parts; comprehensive fruit and vegetable fermentation powder 8-15 parts; soybean peptide powder 3-10 parts; comprehensive digestive enzyme 05-3 parts; and pharmaceutically or dietetically acceptable excipients; the plant selenoprotein enzyme peptide composition, the effervescent tablets and the application thereof are scientifically combined with organic selenium (selenium-rich shepherd's purse powder), active enzymes and small-molecule metabolites generated by fermentation of various fruits and vegetables, soybean peptides easy to absorb and prebiotics. Selenium is the core of the antioxidant system, peptides and fermentation products provide nutritional substrates and regulatory signals, prebiotics improve the intestinal microenvironment, and multiple components synergistically act on multiple pathways such as immunity and metabolism.

The application discloses a kind of pectinatocarpus selenium protein peptide and albumin peptide formula and preparation method, the core active ingredient of the formula is the selenium protein peptide of pectinatocarpus source and egg white albumin peptide, wherein the number average molecular weight of selenium protein peptide is 461Da, total selenium is 412.67mg / kg and selenocysteine and pectinatocarpus plant selenium peptide ratio is more than 90%, and the biological potency of albumin peptide is 94, and the molecular weight is 200-800Da;The application promotes active transport in intestinal tract by albumin peptide as carrier, and is prepared by combining dry granulation and tabletting process, the selenium absorption rate of the application is greatly improved, realizes the synergistic effect of hypoglycemic, hypolipidemic and antioxidant, and provides a new scheme for selenium supplement functional food.

The present application belongs to the technical field of biological detection, and particularly relates to a non-invasive selenium level prediction method based on saliva selenoprotein P detection and application. The present application immunizes mice with heterologously expressed antigen SELENOP (40-194 aa) to generate antibodies, and monoclonal antibodies capable of efficiently recognizing SELENOP are screened and obtained; an ELISA detection method is established using the obtained monoclonal antibodies. The selenium content in human plasma and saliva is detected and analyzed using the detection method and an ICP-MS selenium content detection method, and it is found that there is a significant positive correlation between the three, and a correlation curve between saliva selenoprotein P and human plasma selenium content is established, thereby achieving the purpose of evaluating human selenium level through non-invasive saliva selenoprotein P detection. The technical scheme provided by the present application can be used for large-scale screening and routine health monitoring.

The invention discloses a nano-selenium foliar fertilizer and application thereof in efficient production of plant selenoprotein, the nano-selenium foliar fertilizer can reduce sodium selenite into elemental selenium by adding vitamin C and taking acid-soluble chitosan as a template, so that the content of selenium in the selenium is increased, and the content of selenium in the selenium is increased. The chance that selenium replaces sulfur in methionine and the chance that selenium is combined with other organic components in azolla filliculoidas are increased, and the selenium content of the azolla filliculoidas is greatly increased; the spreadability and adhesiveness of the selenium solution on plant leaves are improved by matching with the surfactant, the selenium solution can be better absorbed by the rumex hanus, cell protoplast flow is promoted by compounding with the plant growth regulator, the cell viability and the selenium utilization rate of plants are improved, and the growth and development of the plants are accelerated.

The utility model relates to the technical field of cultivation devices, in particular to a selenium-containing protein carotenoid microalgae cultivation device. Aiming at the problem that the growth of microalgae is limited due to the change of external illumination and temperature and the inappropriate change of the concentration of carbon dioxide, so that the content of selenoprotein carotenoid is reduced, the following technical scheme is provided: the device comprises a box body, the top of the box body is rotatably connected with a box cover, and a cultivation groove is formed in the box body; a cultivation tank is placed in the cultivation groove, and microalgae are contained in the cultivation tank; the constant-temperature heating unit is mounted in the box body, and the top of the constant-temperature heating unit is in contact with a cultivation tank; the lamp strip is mounted on the inner wall of the cultivation tank and surrounds the outer ring of the cultivation tank. The device can automatically adjust the illumination, the temperature and the carbon dioxide content of microalgae breeding, improves the growth efficiency of microalgae, and ensures the growth of microalgae, the accumulation of nutritional ingredients and the final harvesting efficiency.

The invention discloses a microbial selenium-rich polypeptide protein feed, and relates to the technical field of selenium-rich protein feeds. The feed specifically comprises the following components in percentage by mass: 5-20% of selenium-rich polypeptide, 5-20% of protein, 5-20% of trace elements, 5-20% of vitamins, 5-20% of dietary fibers, 5-20% of metabolites and 5-20% of an antioxidant. 30%-50% of protein; 0.5%-5% of trace elements; 0.1%-5% of vitamins; 8%-25% of dietary fibers; 3%-10% of metabolite; and 0.5%-5% of an antioxidant. The feed additive can effectively enhance the immunity of animals, promote growth and digestion and improve the meat quality and the product quality, is rich in selenium peptide, selenomethionine, glutathione and other components, has a remarkable anti-oxidation effect, can relieve oxidative stress and protect cell health, can effectively improve the feed conversion rate of the animals, and improves the feed utilization rate of the animals. Therefore, healthy growth and economic benefits are promoted.

The invention discloses a compound feed for cage-rearing laying hens to produce selenium-rich eggs as well as a preparation method and application of the compound feed. The compound feed comprises the following components in percentage by weight: 50%-65% of corn, 10%-20% of soybean meal, 5%-15% of rumex hanus powder, 3%-10% of fish meal, 1%-5% of a selenoprotein nutrient solution, 2%-8% of glucose, 1%-3% of zeolite powder, 5%-9% of stone powder, 1%-1.5% of calcium hydrophosphate, 0.2%-0.4% of table salt and 0.5%-1% of a laying hen premix. The preparation method comprises the steps of raw material crushing, mixing, selenoprotein nutrient solution spraying, final mixing and the like. The method is characterized in that through the synergistic effect of the rumex hanus powder, the selenoprotein nutrient solution, the glucose and the zeolite powder, the deposition efficiency and stability of selenium in the eggs are remarkably improved. The feed is fed to laying hens (for example, the laying hens are 300 days old) in the egg producing period, selenium-rich eggs with the selenium content reaching 0.42 mg / Kg can be stably produced, the national standard is met, and the feed is high in safety and easy to popularize.

The invention relates to the technical field of extraction of microbial functional components, in particular to selenium polypeptide with high antioxidant activity as well as a preparation method and application of the selenium polypeptide. The selenium polypeptide provided by the invention is a selenium polypeptide substance with more prominent yeast source high biological activity compared with selenium-enriched yeast, the ABTS free radical scavenging rate reaches 65.51-80.23%, the antioxidant activity under the isoconcentration condition is 5.24-6.42 times of that of total selenium polypeptide, 11.99-14.69 times of that of selenium-enriched protein and 11.18-13.69 times of that of the selenium-enriched yeast, the selenium content is 3646.96-4919.69 mg / kg, and the content of selenium in the selenium-enriched yeast is more than that of the total selenium polypeptide. Compared with selenium-enriched yeast, the selenium-enriched yeast has the advantages of high activity and high absorption efficiency, and has a wide application prospect in the aspects of medicine, functional food, cosmetics and the like.

This invention discloses a serine tRNA synthetase mutant and its applications, belonging to the field of enzyme proteins. This invention utilizes machine learning techniques such as AlphaFold3 to predict SerRS and tRNA... Sec The interaction interface, thereby precisely locating the SerRS that affects tRNA Sec By identifying key amino acid residues and employing a semi-rational mutation strategy, SerRS key sites were targeted and modified to enhance their binding to tRNA. Sec By combining binding affinity and catalytic efficiency, a high tRNA binding affinity and catalytic efficiency were constructed. Sec The SerRS variant with aminoacylation activity improves the supply level of Sec, enabling efficient and high-fidelity expression of selenoproteins, eliminating the mis-incorporation of Ser, and providing a new technical means for the biosynthesis of selenoproteins.

The invention belongs to the technical field of medical biological detection, and provides application of selenoprotein P as a target spot in tumor diagnosis and treatment. The invention specifically provides application of a reagent for detecting the expression level of selenoprotein P in preparation of a tumor diagnosis kit, provides a corresponding detection kit, and further provides application of the reagent as a target spot in preparation of tumor treatment drugs and a corresponding pharmaceutical composition. The invention discloses a key function of the Selenop in interaction of the RTMs and the tumor cells, and the key function is specifically shown as that the Selenop is transferred from the RTMs to the tumor cells, lipid oxidation is inhibited, and the tumor cells are protected from ferroptosis. Based on the discovery, the invention provides an application prospect of Selenop as a tumor treatment target, and provides a potential strategy and method for tumor immunotherapy.

The present application relates to a high selenium-tolerant tree tongue mutant strain and its application, belonging to the technical field of fungal breeding. It discloses a high selenium-tolerant and selenium-rich tree tongue mutant strain YL108, which has been preserved in China Center for Type Culture Collection (CCTCC) in Wuhan University, Wuhan, China, classified as Ganoderma applanatum YL108, with the preservation number CCTCC NO: M 20221347 and the preservation date of August 29, 2022. The strain can maintain normal growth in a culture medium with a selenium content of 90 mg / L, and the mycelium obtained has no red selenium phenomenon, and the amino acid form selenium content in the mycelium protein is up to 511 mg / kg, accounting for about 55% of the total protein selenium. The above-mentioned high selenium-tolerant tree tongue mutant strain Ganoderma applanatum YL108 is more suitable for cultivation in a high-selenium-content medium, and high-quality selenium-containing protein with high amino acid form selenium is obtained, which is a high-quality germplasm resource for preparing fungal selenium-rich products.

The present invention relates to the field of biomedicine technology, and in particular to a method for preparing a thyroid immunomodulatory drug based on a selenoprotein-modified astragalus polysaccharide derivative. The method comprises the following steps: in a first reactor, adding astragalus polysaccharide and maleic anhydride to a Tris-HCl buffer for reaction to obtain olefinated astragalus polysaccharide; in a second reactor, adding food-grade selenoprotein and 2-iminothiolane hydrochloride to a Tris-HCl buffer for reaction to obtain thiolated selenoprotein; pouring the second reactor into the first reactor, adding cysteine ​​to obtain a selenoprotein-modified astragalus polysaccharide derivative; mixing the selenoprotein-modified astragalus polysaccharide derivative, starch, and magnesium stearate, and pressing to obtain the thyroid immunomodulatory drug; the thyroid immunomodulatory drug prepared by the present invention has a positive effect on the immunoregulation of thyroid FRTL-5 cells.

This invention discloses the application of selenoprotein M in the preparation of drugs for treating abdominal aortic aneurysms. This invention also provides the application of selenoprotein M in the preparation of drugs for reducing macrophage inflammation. This invention can effectively delay the progression of abdominal aortic aneurysms and reduce macrophage inflammation, and has broad prospects for medical applications.

The utility model relates to the technical field of extraction devices, and discloses a selenoprotein centrifugal extraction device capable of reducing residue, which comprises an extraction barrel, the bottom of the extraction barrel is fixedly connected with supporting legs, the top of the extraction barrel is provided with a cover plate, the top of the cover plate is provided with a hole, and the inside of the hole is rotatably connected with a circular plate. A turnover mechanism is arranged in the extraction barrel, and the turnover mechanism comprises a U-shaped plate I, a rotating rod I, a placement barrel and a rotating rod II; raw materials are poured into the containing barrel, then a proper amount of leaching liquor is added into the extraction barrel, the containing barrel can be driven to rotate for centrifugal extraction, the leaching liquor is discharged after extraction, the first motor is started to drive the first connecting shaft rod to rotate, the first connecting shaft rod drives the first rotating rod to rotate through the first bevel gear and the second bevel gear, and the first rotating rod is driven to rotate through the second bevel gear. The first rotating rod drives the containing barrel to rotate, so that the containing barrel is turned over, the opening faces downwards, then the containing barrel is driven to rotate, liquid can be fully thrown out, residues are reduced, and resource waste is avoided.

The invention provides an organic selenium soil heavy metal degradation agent and application thereof, and belongs to the field of agricultural planting. The organic selenium soil heavy metal degradation agent is prepared from the following raw materials: organic selenium, selenoprotein, amino acid, high-purity peptide, a biological insecticidal bacterium agent and an organic silicon additive. By adding the organic selenium, changing the occurrence form of the heavy metal, forming an organic compound of the organic selenium and the heavy metal, or replacing a selenium element in the organic selenium with the heavy metal in an ionic state, forming the organic compound of the heavy metal, so that the activity of the heavy metal pollutants is reduced, small molecules of the heavy metal pollutants are converted, and protein selenium is synthesized and enzymolyzed; therefore, the heavy metal decomposition effect is achieved. According to the present invention, the national selenium-rich agricultural product standard can be met after the rice is applied once, the cadmium accumulation in the rice can be reduced, the pesticide residue can be reduced, the soil can be improved, the fungi, the bacteria, the virus bacteria and the fusarium in the soil can be well solved, the soil can be dried without pollution, and the selenium-rich healthy high-quality functional agricultural product can be obtained.

This invention relates to the field of *Paenibacillus polymyxa*, addressing the problem of low production of immune signaling proteins in existing *Paenibacillus* strains. It provides a new *Paenibacillus polymyxa* strain, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC NO.36323. This invention provides a novel *Paenibacillus* strain that possesses both high production of immune signaling proteins and natural biocontrol and growth-promoting properties. It can secrete polymyxin-like antibacterial substances and simultaneously synthesize PR proteins and selenomethionine, overcoming the limitations of traditional *Paenibacillus* strains that only inhibit bacterial growth and lack immune signal transduction. The preparation method provided by this invention involves the strain converting sodium selenite into nano-selenium, which combines with the secreted PR protein to form nano-selenoprotein. Simultaneously, it synergistically produces polymyxins, achieving a dual effect of immune signal activation and direct bacterial inhibition, solving the problems of weak signaling and lack of synergistic antibacterial effects of traditional inducers.

The present invention features compositions and methods for treating selenoprotein-associated diseases. Non-limiting examples of selenoproteins include selenoprotein N (SELENON).

The utility model relates to the technical field of food material selenoprotein extraction, in particular to equipment for extracting high-selenium food material selenoprotein, which comprises an extraction tank, a bottom shell is movably mounted at the bottom end of the extraction tank, and a sealing mechanism is arranged at the joint of the extraction tank and the bottom shell; a movable disc is movably mounted at the upper end of the interior of the extraction tank, and a driving mechanism is arranged between the movable disc and the upper end of the extraction tank. Through cooperative use of the sleeves, the piston rod, the spring, the movable disc and the corrugated pipe, the transmission shaft controls the cutting knife to cut up food materials falling at the upper end of the filtering disc, then clear water is pumped from the outside, enters the flow dividing pipe from the water inlet pipe and is divided into the two sleeves through the flow dividing pipe, and the clear water presses the piston rod downwards to cut up the food materials. The piston rod controls the movable disc to descend and stretches the spring, food material residues remaining on the inner wall of the extraction tank are scraped, filtered by the filtering disc and then discharged, in this way, lifting can be controlled through water pressure, and the equipment cost and the processing cost are reduced.

The invention discloses a three-selenium composition for promoting proliferation of beneficial bacteria and a preparation method thereof, the three-selenium composition is characterized in that the three-selenium composition is prepared from selenoprotein prepared from mulberry leaves, selenium-rich tea leaves and cardamine violifolia as raw materials, nano selenium-rich yeast and L-selenium-methyl selenocysteine, the components of the three-selenium composition are unique in preparation, the three-selenium composition can generate a synergistic interaction effect under a specific ratio, and the composition has a good effect on promoting proliferation of beneficial bacteria. The selenium-enriched yeast is an organic selenium component, the obtained product not only can safely supplement selenium, but also has the effects of promoting proliferation of beneficial bacteria and enhancing immunity, and the technical requirements of symbolic components, pesticide residues and heavy metal content of the product are ensured by adopting an ultrasonic enzymolysis and biotransformation method and a process of nanocrystallizing the selenium-enriched yeast and adsorbing and filtering an extracting solution. The product provided by the invention has the advantages of large market demand, wide market demand, wide application prospect, facilitation of promotion of popularization and application of precise selenium supplement, facilitation of promotion of development of agricultural product planting and processing industry, and substantial social and economic benefits.

The present invention features compositions and methods for treating selenoprotein-associated diseases. Non-limiting examples of selenoproteins include selenoprotein N (SELENON).