The present invention discloses a hyaluronic acid-containing pet molar rod composition as well as a preparation method and applications thereof. The hyaluronic acid-containing pet molar rod composition comprises meat powder and hyaluronic acid containing thallus crushing substances or salts thereof; the composition uses viscosity of hyaluronic acid and thallus crushing substances to replace starch, gel and other adhesives or forming agents, and after the molar rod is prepared, due to rich hyaluronic acid, the molar rod can inhibit bacteria, diminish inflammation, remove halitosis, protect gingiva and oral mucosa and also has the health-care effects of brightening hair and strengthening bones after being digested and absorbed, the unicity of the function of a traditional molar rod is improved, thalli are not removed in the preparation process, cost is saved, resources are fully utilized, and the molar rod has better nutrition and palatability; the effect of an adhesive can be achieved;and more importantly, the crushed thallus can form relatively hard particles after being dried, so that residues, tartar or dental plaque on the teeth of the pet can be effectively removed.