176 results about "Chlamydomonas" patented technology

The invention discloses an expression system and constructing PHB transgene algae method of rhine chlamydomonas exogenesis gene, which comprises the following parts: promotor, exogenesis-goal gene, screening badge and rhine chlamydomonas acceptor algae strain, wherein the method comprises the following: A, selecting and cultivating acceptor algae strain; B, cloning key enzyme gene by PHB biosynthesizing; C, expressing carrier of PHB biosynthesizing key enzyme rhine chlamydomonas; D, using PHB biosynthesizing key enzyme to lead in rhine chlamydomonas from fungus Bacillus alcaligenes with bead-milling method, gene-gun method or electrization; obtaining bivalence or transgene Chlamydomonas of producing PHB by screen selecting and molecule detecting. The poly-hydroxybutyric acid is compounded by acting in conjunction of PHB compounding key enzyme of phbA, phbB and phbC gene coding, which uses acetylcoenzyme A as substrate by photosynthesis. The method simplifies the operating, which reduces cost.

The invention relates to the field of Rapana venosa artificial breeding, in particular to a Rapana venosa ecological breeding method. The method comprises: transferring egg bags into a plastic basket or net cage (10-20mesh) for suspended hatch in a nursery pond and charging air continuously, wherein the density of the egg bags is 5,000 to 8,000/m<3>; breeding larvae at a density of 1/ml with bait made of chlamydomonas (diatom and tetraselmis are preferred) in an amount of 1.0 to 12.0*10<4>/ml every day at a water temperature of 22 to 26 DEG C; continuously charging air, changing 1/2 of water every day; and when the larvae grow three whorls to a size of 700 to 800 micrometers, placing the larvae in to 60-mesh net bags, cages or tanks together with double-shell spats (animal baits for transformed Rapana venosa) for sea breeding.

Disclosed is a Volvox carteri-derived light-receiving channel rhodopsin with an improved expression efficiency on a cell membrane. Specifically disclosed is a modified Volvox carteri-derived light-receiving channel rhodopsin protein. The protein is modified to contain an N-terminal region of Chlamydomonas reinhardtii-derived channel rhodopsin-1 at the N-terminal of the Volvox carteri-derived light-receiving channel rhodopsin protein, wherein the N-terminal region is involved in cell membrane-localized expression and contains no transmembrane domain of the Chlamydomonas reinhardtii-derived channel rhodopsin-1.

The invention relates to a biological hydrogen production technology, in particular to a method for improving the chlamydomonas reinhardtii hydrogen production amount of a leghemoglobin ferrous chelate enzyme gene. The traditional chlamydomonas reinhardtii hydrogen production method has the defects that chlamydomonas reinhardtii hydrogen enzymes are sensitive to oxygen and easily restricted by the oxygen to lose activity, and the chlamydomonas reinhardtii hydrogen production effect is limited. The invention discloses an application of a leghemoglobin ferrous chelate enzyme gene and a globulin subunit gene to hydrogen production by constructing the leghemoglobin ferrous chelate enzyme gene hemH and the globulin subunit gene lba in a chlamydomonas reinhardtii chloroplast expression vector, transferring the expression vector in the chlamydomonas reinhardtii chloroplast and expressing the hemH-lba gene in the chlamydomonas reinhardtii chloroplast. The invention has the advantages that the content of oxygen in a closed culture system of the transformed chlamydomonas reinhardtii is reduced obviously faster than that of the chlamydomonas reinhardtii of an un-transformed gene, the oxygen content is kept at a lower level, and the hydrogen production amount is obviously increased.

The invention discloses a hanging bag-type chlamydomonas culturing bracket. The hanging bag-type chlamydomonas culturing bracket comprises at least two upright posts and a beam fixed to the top ends of the upright posts, wherein 3 to 5 supporting rods are arranged on the beam; a lamp hanger in which lamp tubes are arranged is arranged under the beam between two adjacent upright posts; a feed liquor inlet pipe and an algal liquid discharge pipe are arranged below the beam in turn; a breathing pipe is also arranged above the beam; and 6 to 8 lamp tubes are horizontally arranged on the lamp hanger from the top to the bottom. The hanging bag-type chlamydomonas culturing bracket has a simple structure and low cost, is convenient to use and is firm and durable, provides an effective support structure for continuous cultivation of chlamydomonas, realizes layered fixed circuits, waterways and gas circuits, has clear routing, distinct levels, less space occupation and clear wiring conduit in the continuous cultivation of the chlamydomonas, fully utilizes light sources, can support an overall reaction system in all directions, and is convenient to operate and effectively control in a cultivation process of the chlamydomonas.

The invention discloses a screening method for heavy metal cadmium adsorption mutants of Chlamydomonas reinhardtii. The screening method comprises: selecting a suitable screening concentration for heavy metal cadmium adsorption mutants according to the influence of different cadmium chloride concentrations on the growth of Chlamydomonas reinhardtii; Transformation to obtain Chlamydomonas reinhardtii mutants; screening out heavy metal cadmium adsorption mutants of Chlamydomonas reinhardtii, the screening of heavy metal cadmium adsorption mutants of Chlamydomonas reinhardtii includes screening heavy metal cadmium sensitive mutants and screening heavy metal cadmium resistant mutants; identification screening Mutant genes of heavy metal cadmium adsorption mutants of Chlamydomonas reinhardtii. The invention achieves the technical effect of being able to obtain a large amount of Chlamydomonas heavy metal cadmium adsorption mutants simply and efficiently.

The invention discloses a litopenaeus vannamei ecological breeding method based on live food and water quality bio-regulation. The method includes following steps: (1), treating water before breeding,and performing live food culture; (2), arranging larvae; (3), breeding, regulating water quality, and preventing and controlling pest and disease; (4), marking thickness, desalting, and transferring.An ecological breeding technique of chlamydomonas plus artemia plus probiotics plus compound Chinese herbal medicine is provided, and natural protein, trace elements and minerals are provided for theshrimp larvae, so that the shrimp larvae have strong physique and resistivity, and ecological shrimp larvae bred by the method are big in size, orderly in specification, quick in growth and high in survival rate. The breeding method is advanced in concept, scientific, reasonable, easy in implementation and suitable for large-scale application in high-health breeding of litopenaeus vannamei and has good social benefit and economic benefit.

A method for artificial oxytocin and fertilization of Mytilus coruscus comprises the following steps of a parent cultivating pool: building an outdoor cultivating cement pool with the dimension of 10cmx1.5mx1m, stocking inflatable stones, covering canvas above the cement pool to prevent rain and sunlight, and shading the periphery of the cement pool by dark cloth to regulate strength of light; selecting Mytilus coruscus which has new and integral shell surfaces, full gonads, complete gill, light red ovaries and white testes, and is free of damage and powerful in closure of double shells; preparing baits, wherein chlamydomonas live baits including cultured Dunaliella viridis, latymonas subcordiformis and Chaetoceros serve as primary baits, and artificial compound feed including spirulina powder, chlorella powder and yeast tablets serves as secondary baits; drying parent Mytilus coruscus with developed gonads in the shade for 6-8 hours, stimulating the parent parent Mytilus coruscus for 1 hour through running water, and feeding the parent parent Mytilus coruscus into prepared warming seawater.

The invention relates to an artificial breeding method for tuba false fusus, which comprises the following steps of: on the basis of investigating tuba false fusus resources along the coast of Hainan and reproductive biology, finding out the thriving propagation season of the tuba false fusus, and catching parent tuba false fusus in the season; putting healthy adult tuba false fusus into a nursery pond for intensified breeding, and optimizing breeding conditions such as water flow, illumination, water temperature, and salinity; after the tuba false fusus is mated and lays eggs, cutting off an egg capsule, and transferring to an incubation basket for flowing water breeding; after young tuba false fusus is hatched, transferring to a breeding basket, feeding concentrated chlamydomonas in first 4 to 6 days, and then feeding fresh clam and Sinonovacula constricta; when the young tuba false fusus grows to 2.0-2.5cm, dividing the young tuba false fusus; and when the young uba false fusus grows to about 4.0cm in the breeding basket, entering a culture stage. Based on the reproductive biological process of the tuba false fusus, the method realizes indoor artificial breeding of the tuba false fusus, shortens the artificial breeding cycle, is suitable for mass production, and has the advantages of simpleness, practicability, convenient operation, low production cost, and high survival rate of the bred tuba false fusus.

The invention relates to exogenous gene expression and biological hydrogen production technologies, in particular to a method for increasing exogenous gene expression quantity and Chlamydomonas reinhardtii hydrogen production quantity by codon optimization. In the prior art, the expression efficiencies of exogenous leghemoglobin genes hemH and lba in Chlamydomonas reinhardtii chloroplast are low, thus influencing the increase of the Chlamydomonas reinhardtii hydrogen production quantity. The invention discloses a method for respectively optimizing the codon bias of the leghemoglobin genes hemH and lba. The codon-optimized hemHc and lbac genes are transferred into the Chlamydomonas reinhardtii chloroplast to be expressed. The invention obviously increases the expression quantity of the exogenous recombinant protein; the expression quantity of the exogenous leghemoglobin hemH and Lba in the Chlamydomonas reinhardtii chloroplast is increased by 6.8 times; and compared with the non-codon-optimized hemH and lba transgenic Chlamydomonas reinhardtii, the Chlamydomonas reinhardtii hydrogen production quantity is increased by 22%. The invention is applicable to regulating and controlling the exogenous gene expression of more species having codon bias.

The invention relates to a biological hydrogen production technology, in particular to a method for breeding high-yield photosynthetic-hydrogen-production chlamydomonas reinhardtii through cell nucleus insertion mutagenesis. The traditional chlamydomonas reinhardtii has low hydrogen production rate and limits the application and the development of a biological and photosynthetic hydrogen production technology. The method for obtaining a high-hydrogen-production mutant strain through the random insertion mutagenesis of chlamydomonas reinhardtii cell nuclei comprises the following steps: cultivation of the chlamydomonas reinhardtii; preparation of a chlamydomonas reinhardtii cell nucleus transformation vector and a glass bead; chlamydomonas reinhardtii cell nucleus transformation and transformant screening; analysis of the hydrogen yield and the oxygen consumption of chlamydomonas reinhardtii transformant hydrogen-production cultivation; analysis of the integration and the expression thereof of ble genes in a chlamydomonas reinhardtii mutant strain; and analysis of the influence of recombinant leghemoglobin hemH-1ba on the growth of the chlamydomonas reinhardtii. The invention has the advantages that the biological hydrogen production yield of the obtained high-hydrogen-production chlamydomonas reinhardtii is 7 times of the original yield; the method provides a good experimental material and a good basic condition for deeply researching the hydrogen-production metabolic mechanism of the chlamydomonas reinhardtii.

The invention aims to provide a method for breeding apostichopus japonicus selenka. The method comprises the following steps of: placing oosperms of the apostichopus japonicus selenka in an hatching slot for hatching; breeding apostichopus japonicus selenka larvae in a breading tank after small auricularia larvae are produced by hatching the oosperms; putting attaching bases for collecting young apostichopus japonicus selenka when 20 to 30 percent of the apostichopus japonicus selenka larvae grow into doliolaria larvae; and screening and taking the young apostichopus japonicus selenka out of the tank when the young apostichopus japonicus selenka grow to the length of between 2 and 4cm, and transferring the young apostichopus japonicus selenka into a pool for breeding. The apostichopus japonicus selenka bred in the pool is not required to be fed, and natural basic bait organisms brought by water changing are taken as feeds such as chlamydomonas and benthic diatoms primarily. The apostichopus japonicus selenka is harvested by an artificial diving fishing method when growing to the weight of between 4 pieces/kg and 6 pieces/kg.

The invention relates to a soilless Nereid larva breeding method. According to the method, with 27-29 DEG C of water temperature, 7.8-8.6 of pH and 28-30 of salinity, larva breeding water undergoes dark precipitation and secondary sand filtration and is then filtered for further use by a 300-mesh bolting-silk bag; large and vigorous heteronereis bodies with no exterior wounds are selected and positioned in a plastic bucket containing 0.5 m3 of sea water for oviposition and insemination according to a proportion of 3:1 between the female and the male heteronereis bodies; oosperms undergo three times of egg washing and then are moved into a larva breeding pond; larva in a roaming period are mainly fed with chlamydomonas together with a certain amount of artificially mixed feed; when the larva grow into five-setiger nectochaeta larva, gracilaria is put in the pond as an attachment substrate, and a small amount of fish paste is also fed; and during the Nereid larva breeding, according to larva growing periods, the light illumination is controlled in a range of 300-1000 Lux. When the gracilaria is used for replacing silt as the Nereid perching place, as the gracilaria can utilize the metabolic products of the Nereids as the nutrients to promote self growth and is easily cleaned, the living environment of the larva can be bettered, the diseases and the damage to the larva during larva generation can be reduced, and the survival rate of the larva can be improved.

The invention relates to an anti-stress health agent for pomfret. The anti-stress health agent for pomfret comprises, on the basis of weight percentage of dry matters, 15 to 20% of vitamin C, 5 to 10% of taurine, 3 to 5% of gamma-aminobutyric acid, 10 to 15% of Chlamydomonas powder and 30 to 40% of chitin, with the balance being a diluent. The above mentioned components are uniformly mixed and then added into a feed for pomfret, wherein the components account for 1 to 4% of the total weight of the feed. The anti-stress health agent provided in the invention has good anticonvulsant and tranquillization-promoting effects, enables the survival rate of cultured pomfret to be obviously increased, improves the feeding rate of pomfret and promotes growth of pomfret; and the agent has the advantages of stable sources of raw materials, low price and a simple production process.

A process for increasing the hydrogen releasing efficiency of chlamydomonas features use of the dominant complementation between phycobionts.

The invention discloses a method for improving hydrogen production of chlamydomonas. The method includes steps of commonly cultivating chlamydomonas and nitrogen-fixing bacteria; before commonly cultivating, cultivating chlamydomonas to a saturated period and cultivating nitrogen-fixing bacteria to a logarithmic phase, wherein the nitrogen-fixing bacteria is round brown nitrogen-fixing bacteria; the chlamydomonas is Chlamydomonas reinhardtii cc849 (with cell wall type) and Chlamydomonas reinhardtii cc124 (cell wall defective type). The method can effectively improve the hydrogen production of chlamydomonas; in relative to the hydrogen production of independent chlamydomonas, the maximum hydrogen production can be improved by 16 times.

The invention relates to a soilless Nereid breeding method. A cement pond is used as a Nereid breeding pond. Sea water undergoes dark precipitation and secondary sand filtration and is then filtered for further use by a 300-mesh bolting-silk bag. Gracilaria is selected as a Nereid perching environment. The breeding pond and the gracilaria are sterilized. The gracilaria is paved at the bottom of the pond. The filtered sea water is added. Yong Nereids with 2-3-meter bodies are put in the pond according to a density of 1500-2000 Nereids per square meter. During the Nereid breeding, chlamydomonas, artificially mixed feed and fish paste are used for feeding the Nereids. Water in the pond is changed each day. The breeding pond is cleaned regularly. The light illumination is controlled in a range of 400-1000 Lux. Meanwhile, as the Nereid grow, the number of the Nereids is added. After 8-10 months of breeding, the Nereid yield rate can reach 5-7 kg/m2, which is 40 percent higher than the traditional breeding methods. The method adopts the gracilaria in place of silt as the Nereid perching place, thereby facilitating the cleaning, reducing a great amount of man power, fully improving the breeding environment, decreasing the possibility of diseases and being good for the growth of the Nereids and the breeding density increase. Meanwhile, the gracilaria can also be sold as commodity.

The present invention relates to a kind of saline alga photosynthetic metabolic pathway key enzyme NADP-glyceraldehyde-3-phosphate dehydrogenase gene, coded protein and its clone and protein expression method. The invented saline alga photosynthetic metabolic pathway key enzyme NADP-glyceraldehyde-3-phosphate dehydrogenase gene has the base sequence showed by SEQ NO 5, and its coded protein has the amino acid sequence showed by SEQ NO 6. Said invention uses homologous fragment of glyceraldehydes-3-phosphate dehydrogenase gene originated from chlamydomonas as probe and firstly clones a glyceraldehydes-3 phosphate dehydrogenase specialized by saline alga. Besides, said invention makes primary analysis for its sequence and coded protein sequence, at the same time makes primary function analysis for said gene.

The invention relates to a chlamydomonas reinhardtii lipid metabolism gene CrDGAT2-5 (Chlamydomonas reinhardtii diacylgycerol acyltransferase2-5), an encoding protein thereof, and an application thereof. The chlamydomonas reinhardtii lipid metabolism gene CrDGAT2-5 is obtained from cDNA amplification with an au.g4218_t1 gene disclosed by JGI chlamydomonas reinhardtii gene database as a template. The sequence of the nucleotide is represented by SEQ ID NO:1. The protein sequence of CrDGAT2-5 encoding protein is conjectured from the base sequence of CrDGAT2-5, and the protein has an amino acid residue sequence represented by SEQ ID NO:2. The gene can be used in chlamydomonas reinhardtii plant expression, and assists in substantially improving the neutral lipid content and the biomass of chlamydomonas reinhardtii. According to the invention, the au.g4218_t1 gene disclosed by JGI chlamydomonas reinhardtii gene database is adopted as the template, and cDNA amplification is carried out, such that the chlamydomonas reinhardtii lipid metabolism gene CrDGAT2-5 is obtained. When used in chlamydomonas reinhardtii plant expression, the gene assists in substantially improving the neutral lipid content and the biomass of chlamydomonas reinhardtii. Therefore, the invention has a great significance in the improving of microalgae oil content and quality.