The application discloses a
Sinonovacula constricta
glycoprotein nano-
enzyme Sc-NZ and a preparation method thereof, and particularly relates to the technical field of nano-enzymes. The
Sinonovacula constricta
glycoprotein nano-
enzyme Sc-NZ is a cluster-shaped
nanoparticle obtained by treating a product separated and purified from
Sinonovacula constricta under ultrahigh pressure, and the
particle size distribution is 30-50 nm. The preparation method comprises the following steps: taking
soft body tissue of Sinonovacula constricta, homogenizing and
defatting, centrifuging to collect the precipitate to obtain defatted
powder, extracting the defatted
powder by an alkali extraction and
alcohol precipitation method, adjusting the pH to 4.0-5.0, collecting and
drying the precipitate to obtain a crude
glycoprotein; subjecting the crude glycoprotein to enzymolysis, and then sequentially subjecting the glycoprotein to
anion exchange chromatography and gel
filtration chromatography to collect an
active component to obtain a Sinonovacula constricta natural glycoprotein; adding deionized water or a
phosphate buffer to the Sinonovacula constricta natural glycoprotein to prepare a solution, and placing the solution in an ultrahigh pressure device to obtain the Sinonovacula constricta glycoprotein nano-
enzyme Sc-NZ. The Sinonovacula constricta glycoprotein nano-enzyme Sc-NZ has the advantages of being prepared by green physical modification,
high activity, high stability and multifunction.