260 results about "Dunaliella salina" patented technology

Disclosed is a method for making a bioreactor comprising a foreign target gene, special selectable markers and Dunaliella Salina as host. It is prepared by the genetic transformation techniques that include introducing a foreign target gene into the cells of Dunaliella Salina and screening the transformed cells of Dunaliella Salina. The bioreactor of the present invention can be used as a safe and cheap production system for proteins of pharmaceutical interest including vaccines, especially oral products, in a large scale, because the cells of Dunaliella Salina are easy of genetic manipulation in preparation of the bioreactor, nontoxic and edible for humans and animals, and harmless to the environment.

The invention provides five-alga probiotic enzyme tablets and a preparation method thereof. The five-alga probiotic enzyme tablets are prepared from the following raw materials in parts by mass: 10-20 parts of chlorella vulgaris, 1-10 parts of dunaliella salina, 1-10 parts of euglena, 1-10 parts of red algae, 10-20 parts of spirulina and 10-20 parts of probiotic powder. The five-alga probiotic enzyme tablets provided by the invention adopt the chlorella, dunaliella salina, red algae, spirulina and euglena for complementation in nutrition, and obtain trace elements easier to absorb by a human body through probiotic fermentation, thereby helping to reinforce the immunologic function of the human body, resist virus infection and proliferation, inhibit cancer cell proliferation, repair the damage of an organism quickly, remove vivotoxin, suppress blood pressure and blood sugar rise, reduce the serum cholesterol content, and thus improve the comprehensive conditioning of the human body.

The invention discloses a large-scale cultivation method for marine floating harpacticoida for improving the cultivation efficiency, reducing the production cost, and providing reliable food supply for grow seedlings of marine economic animals. The method comprises the following steps that: the salinity of water for cultivating the harpacticoida is 20ppt, the temperature is 24 DEG C, the dissolved oxygen of the water is more than 5 milligrams per liter, the pH is between 7 and 8, the bait is at least one of isochrysis zhanjiangensis, dunaliella salina and chaetocetros muelleri, the initial density of the harpacticoida cultivation is 50 to 100 ind. / l cultivation water, the supply amount of the bait is 5 to 10 liters every day, 300 liters of cultivation water is updated every day, and the harpacticoida is cultivated till the density of the harpacticoida is at least 1,500 ind. / ml; and the harpacticoida in 500 liters of water is harvested every day and the fresh cultivation water is supplied, the using amount of the bait is 10 to 15 liters every day, and the harpacticoida is continuously cultivated for 30 to 50 days.

The invention discloses a deer-horn gelatin health product for conditioning eight types of abnormal body constitution. The deer-horn gelatin health product comprises the following raw materials in parts by mass: 1-10 parts of deer-horn gelatin powder, 1-10 parts of panax ginseng, 1-10 parts of cordyceps militaris, 0.1-4 parts of tissue culture of saussurea involucrate, 1-6 parts of polygonatum sibiricum and 1-4 parts of radix polygonati officinalis, into which the following materials can be added: sanguis cervi, deer bone meal, lepidium meyenii walp, moringa oleifera leaves, earthworm protein, noni berry smoothie, Chinese wolfberries, fructus mori, phyllanthus emblica, dunaliella salina, white hyacinth beans, arillus longan, semen cassiae torae, lilium brownie, semen euryales, hippophae rhamnoides, alpinia oxyphylla, fructus jujubae, herba elsholtziae, semen ziziphi spinosae, poria cocos, fructus citri sarcodactylis, citrus medica, exocarpium citri rubrum, peach kernels, tortoise, coix seeds, lonicera japonica, licorice, semen ginkgo, Chinese yam, carica papaya, myristica fragrans, pollen pini, rosa roxburghii, eucommia staminate flower, flos rosae rugosae, euryale ferox, linderae aggregate leaves, honey, krill oil, sacha inchi oil, wheat oligopeptides, yeast beta-glucosan, milk minerals, tomato seed oil and eucommia seed oil. The deer-horn gelatin health product provided by the invention has a good curative effect for people with qi-blood deficiency and viscera function failures and for low-immunity sub-healthy groups with all kinds of deficiency syndromes.

The invention discloses a health care product containing Noni juice. The health care product is prepared from the following raw materials in parts by weight: 1-10 parts of Noni juice, 0.1-2 parts of dunaliella salina, 1-10 parts of poria cocos, 1-8 parts of coix seed, 1-6 parts of semen cassiae, 1-6 parts of chrysanthemum, 1-6 parts of fructus amomi (which is added or not added), 1-5 parts of mesona blume, 1-8 parts of barbary wolfberry fruit, 1-6 parts of gynura procumbens, 0.1-2 parts of tissue culture of saussurea involucrata, 1-5 parts of inulin, 1-6 parts of honeysuckle, 1-4 parts of hovenia dulcis thumb, 1-6 parts of cape jasmine fruit, 1-6 parts of folium mori, 1-5 parts of lophatherum gracile, 1-8 parts of the root of kudzu vine, 1-6 parts of dandelion, 1-4 parts of mint, 1-6 parts of fingered citron, 1-5 parts of citron, 1-6 parts of gorgon fruit, 1-5 parts of Chinese mosla herb, 1-5 parts of vermilion, 1-5 parts of lotus leaves, 1-8 parts of rhizoma polygonatum, 1-7 parts of wild jujube seeds, 1-7 parts of raspberries, 1-4 parts of male flowers of eucommia ulmoides oliv and 0.1-2 parts of cordyceps militaris. The health care product is skillfully collocated in the five aspects of the heart, liver, spleen, lung and kidneys according to the principle of treating hot and humid stagnation and applying on sanjiao (triple energizers), so that the body is comprehensively and harmoniously conditioned, and diseases can be controlled; after the health care product is taken for a long time, the damp and hot physique can be conditioned, and the body is effectively promoted to reach a state of balance of yin and yang.

The invention discloses a solid beverage for aiding blood pressure reduction and blood sugar reduction and a preparation method thereof. Main effective components comprise one or more of the following raw materials: potassium alginate (low-molecular-weight potassium alginate), hawthorn extract, wheat oligopeptide, horseradish tree leaf powder or horseradish tree leaf extract, red yeast rice powder, natto powder, oat beta-glucan, chitooligosaccharide, dunaliella salina and extract thereof, bamboo leaf flavone and kudzu root or kudzu root extract; and auxiliary materials comprise one or more of the following raw materials: inulin, resistant dextrin, bean powder, tea powder, milk powder, barley leaf powder, citrus fiber powder and coffee powder. The preparation method comprises the following steps: 1, pulverizing and screening; 2, weighing; and 3, mixing and stirring. The solid beverage disclosed by the invention is favorable in safety, has no side effect, and avoids complications and side effects caused by long-term western medicine taking; the solid beverage is convenient to eat, easy to carry, rich in nutrient and favorable in taste; and the solid beverage has a definite action mechanism of aiding blood pressure reduction and blood fat reduction, is economical and practical, and has a wide application range.

The invention relates to the technical field of genetic engineering, in particular to a homologous recombination empty carrier of dunaliella salina chloroplasts and an application of the homologous recombination empty carrier. The carrier comprises a promoter and a terminator, wherein the recombination empty carrier contains an upstream homologous arm with a base sequence represented as SEQ ID NO:1 and a downstream upstream homologous arm with a base sequence represented as SEQ ID NO:2, and a base sequence which is represented as SEQ ID NO:5 and constitutes a polycistron structure with at least one exogenous gene is inserted between the homologous arms. With the adoption of the dunaliella salina chloroplast stable expression system, stable expression of multiple exogenous genes in chloroplasts can be realized.

The invention belongs to the field of cosmetics and in particular relates to a whitening composition and a preparation method thereof. The whitening composition disclosed by the invention comprises the following components: a scutellaria baicalensis extract, hydrolyzed prunus domestica, mushroom glucan, a narcissus extract, 377, a dunaliella salina teodoresce extract, a glycyrrhiza glabra extract and deionized water. According to the whitening composition disclosed by the invention, the main whitening active ingredients refer to the natural plant extracts, preservatives and other chemical additives with large side effects and high irritation are not added, and the whitening composition is prepared by adopting multiple skin-beautifying active matters and has the characteristics of synergy, high systematicness and mutual coordination, so that a good whitening effect can be achieved under the composition by virtue of low content of a single component, and the adverse reaction rate is low. According to the whitening composition disclosed by the invention, the whiteness of the skin and skin color uniformity can be obviously improved, and the problems such as dark color, spots and roughness are eliminated, so that the skin can restore to be in a white, tender and uniform healthy skin color state.

The invention relates to a separation and purification method for Dunaliella salina polysaccharide, which belongs to the technical field of salina biotechnology and the product and chromatographic fractionation thereof. The method comprises the following steps: using beta-carotene extracted Dunaliella salina powder as the raw material and performing hot water extraction on alkaline condition, removing most part of protein by the isoelectric point method, separating out polysaccharide by the ethyl alcohol precipitation method, removing the residual protein by the Sevag method, performing separation and purification by the ion-exchange chromatography-phosphate buffer ionic strength gradient elution method in combination with gel filtration chromatogram-NaAc aqueous solution gradient elution method, collecting separately, freeze drying after appropriate concentration, and finally obtaining five homogeneous components of Dunaliella salina polysaccharide, including sulphatized heteropolysaccharide containing galactose as the main part and high-content arabinose and xylose, and nucleic acid polysaccharide. The method has the advantages of simplicity, practicability and low cost, thereby being favorable for promotion, development and application.

The present invention relates to compositions and uses of a novel Dunaliella salina HT04 microorganism. In addition, the present invention relates to novel methods for culturing harvesting, preservation, production of algae seedstock and uses thereof.

The invention discloses a seawater domestication method for Daphniopsis tibetana Sars, which comprise the following steps: disinfecting the culture container, culture container being opaque in periphery; concocting culture liquor, taking disinfected and filtered seawater to adjust pH value to 7-8, temperature 10-15 degree, salinity 10-15, selecting Daphniopsis tibetana Sars and placing it in container, culturing it with above-mentioned liquor, illumination intensity 1000-2000Lx, photoperiod 14D:10L, feeding with Chaetoceros sp. feedstuff, feeding volume 30x105cell / ml, selecting healty, active female Daphniopsis tibetana Sars which has produced baby Daphniopsis tibetana Sars and is 2-3 mm in body length, feeding it with algae liquid selecting one or two kinds from Chaetoceros sp., Dunaliella salina, Isochrysis galbana, chlorella, feeding density 30x105cell / ml, inoculating Daphniopsis tibetana Sars density 100ind / l, observing Daphniopsis tibetana Sars growth state and water body to partly for wholly substitute for Artemia and promote fish fry survival rate, reduce the seedling-breeding cost.

The invention discloses a fast culture method for dunaliella tertiolecta, belonging to the field of aquaculture. Conventionally, dunaliella tertiolecta which is cultured by using an open track cement pool and a f / 2 culture solution formula is slow to grow, easy to pollute, low in yield, low in content of beta-carotene, poor in quality and low in efficiency. In order to overcome defects of the conventional culture method, the dunaliella tertiolecta is cultured by using an illumination incubator and nutrients such as ammonium nitrate, urea, monopotassium phosphate, sodium hydrogen carbonate, ferric citrate, vitamin B1, vitamin B12, 920 plant growth stimulin and the like are further added into the nutrient solution, nutritive salt is supplemented in the later stage of culture, the illumination intensity and temperature are enhanced, and continuous growth of algae cells is promoted and beta-carotene is stressed to be accumulated, so that the dunaliella tertiolecta is fast to grow, high in yield, hard to pollute, good in quality, simple and convenient to operate, less in investment and high in efficiency.

The ecological mixed feed for Razor clam is prepared with 16 kinds of materials, including white fish meal, soybean dregs, peanut dregs, scallop leftover, shrimp head powder, etc in certain weight proportion. The ecological mixed feed for Razor clam has no chemical additive, high conversion rate, less pollution to water, capacity of promoting Razor clam growth, low cost, environment friendship and other advantages, and may be used in raising Razor clam effectively.

The present invention relates to compositions and uses of a novel Dunaliella salina HT04 microorganism. In addition, the present invention relates to novel methods for culturing harvesting, preservation, storage, and production of algae seedstock and uses thereof.

The invention discloses a skin whitening composition. The skin whitening composition contains tranexamic acid, ascorbic acid ethyl ether, vitamin B3, N-(omega-undecylene acyl) phenylalanine, chamomile extract, dunaliella tertiolecta extract (IBR-CLC 0402), methylene bis-benzotriazolyl tetramethyl butyl phenol, coenzyme Q10, purslane extract, alpha-red myrrh alcohol, beta glucan, clove oil, azone, 1,3-butanediol solvent as well as 1,2-octylene glycol and ethylhexylglycerin antiseptics. The active ingredients in the skin whitening composition can inhibit activity of tyrosinase, reduce formed melanin, inhibit migration of melanin globules, block information transmission between keratinocytes and melanocytes, promote falling off of cuticle, absorb ultraviolet rays, eliminate free radicals and resist inflammation. According to the skin whitening composition, each component is significant in synergic whitening effect, and the skin whitening composition is safe and stable and can be applied to skin whitening cosmetics.

The invention provides a preparation method of transgenic dunaliella salina for improving the photosynthesis efficiency, which is characterized by comprising the following steps: colonizing or reforming genes related to carbon fixation and photosynthesis in a photosynthetic autotroph; transferring the genes into a chloroplast genome or nuclear genome of the dunaliella salina for expressing; successively and selectively culturing; and finally obtaining a stable transgenic dunaliella salina strain. By introducing independent or combined expression of high-efficiency genes related to carbon fixation and photosynthesis, the invention improves the concentration and the transferring speed of CO2 in the transgenic dunaliella salina and ensures that the capability of the transgenic dunaliella salina using the CO2 for photosynthesis can be improved, thereby promoting the autotrophic growth of the transgenic dunaliella salina, accelerating and increasing the accumulation of biomass liveweight of dunaliella salina cells and meeting the requirement on scale production of biodiesel and metabolin thereof as well as genetic engineering products.

The present invention relates to an ultrasonic extraction method for beta-carotene in Dunaliella salina powder. The ultrasonic extraction method comprises: adding water to Dunaliella salina powder and cooling, carrying out microwave heating thawing, sequentially carrying out saponification and cooling centrifugation, removing the supernatant, adding an extraction solvent to the sample solution, carrying out ultrasonic extraction, and carrying out cooling crystallization to obtain the beta-carotene extract. According to the present invention, the sample is subjected to freezing-microwave thawing, such that the water content in the sample can be reduced, and the subsequent operation is easy; the saponification is performed on the sample before the extracting, such that the beta-carotene can be completely extracted; the ethyl acetate or 90-95% ethanol aqueous solution is adopted as the ultrasonic extraction solvent and the extraction is performed for about 0.5-0.7 h at the temperature of 25 DEG C, such that the beta-carotene is substantially and completely extracted; and the extraction solvent single, is easy to recover, and has high safety, and the method can be widely used in massive food or medicine and other fields.

The invention relates to the technical field of traditional Chinese medicinal health-care products, in particular to a sea cucumber dunaliella salina glucosan selenium preparation and a preparation process. The sea cucumber dunaliella salina glucosan selenium preparation is characterized by being prepared from the following components in parts by weight: 40 to 45 parts of sea cucumber, 20 to 40 parts of dunaliella salina, 15 to 40 parts of yeast glucan and 0.01 to 0.05 part of selenium carrageenan. The sea cucumber dunaliella salina glucosan selenium preparation has the advantages of enhancing the immunity and the constitution of a human body, improving the memory and the like.

The invention discloses an optimized culture method of Dunaliella salina. According to the method, firstly, a culture medium is prepared in a closed photobioreactor, and the prepared culture medium contains 0.3-0.5 g of NaNO3, 0.01-0.025 g of KH2PO4, 1 mL of Fe-EDTA mother liquor, 1 mL of microelement mother liquor and 1 mL of vitamin mother liquor; then a batch of Dunaliella salina strains cultured to the exponential growth phase are screened, and the culture medium is inoculated with the strains with the inoculation amount ranging from 5% to 15%; finally, culture is performed at the temperature controlled to range from 23 DEG C to 25 DEG C with the illumination intensity of 2000-3000 lx and the illumination time of 12h:12h alternately for 15-20 days, and a culture is obtained. Beta-carotene in the Dunaliella salina cultured with the method is extracted with an ultrasonic method, and the content of the beta-carotene is detected to be increased to be higher than 14.45%.

The invention discloses a method for harvesting dunaliella salina, relates to a microalgae and provides a dunaliella salina harvesting method which is higher in efficiency and lower in energy consumption. The method comprises the steps of 1) at the later period of dunaliella salina cultivation, harvesting when the algae cell density is more than (1-10)*106 / mL; and 2) adding flocculant mother liquid into algae liquid, uniformly stirring, standing, removing the supernatant, and collecting to obtain algae paste, and calculating the flocculation efficiency. The inorganic flocculant is adopted to efficiently flocculate and enrich the dunaliella salina in a high-salinity culture solution. The use amount of the flocculant is determined according the temperature, the salinity, the pH and the cell concentration of the algae liquid, the least use amount cannot be less than 10mg / L, and the largest use amount cannot be more than 2000mg / L. The method is simple and economic, and the inorganic flocculant is utilized, that is, dunaliella salina cells and the flocculant are mutually absorbed, agglomerated, flocculated and deposited under the action of electrostatic forces, intermolecular gravitation, hydrogen bonds and the like, thereby achieving the purposes of separating the algae cells from the culture mediumand and enriching the algae cells. The flocculant is independently added, so that the cost is low, flocculating constituent is formed rapidly, and the deposition speed is high.

The invention discloses a maca dunaliella salina compound preparation. The maca dunaliella salina compound preparation comprises dunaliella salina and spirulina and is prepared from the following materials: 3-10 percent of dunaliella salina, 40-45 percent of spirulina and 45-55 percent of maca. The maca dunaliella salina compound preparation has the effects of building body and tonifying Yang, curing organs, maintaining beauty and keeping young and improving the digestive system, achieves a two-way blood-pressure regulating function, as well as a two-way blood sugar regulating and balancing function, can regulate body metabolism and resist aging, tumor and fatigue and enhance the immunity, and has remarkable effects on cardio-cerebrovascular diseases, chronic diseases, senile dementia, climacteric syndrome and the like. When drunk frequently, the maca dunaliella salina compound preparation increases sexual intercourse, keep youth for a long time and prolongs the lifetime, has the advantages of relaxing tendons and activating collaterals, activating blood circulation to dissipate blood stasis, dispelling wind to eliminate dampness and strengthening bones and tendons and can remarkably improve memory.

The invention discloses a method for constructing a Dunaliella salina chloroplast transformation vector, in particular a method for constructing a dual-exchange dual-selection marker Dunaliella salina chloroplast transformation vector by using a light dependence free protochlorophyllide reductase chlN, chlB or chlL gene as a homological segment, chloramphnicol acetyltransferase (CAT) gene and a resistance gene bar of phosphinothricin (PPT) as a weedicide as screening markers and an atpA promoter and a rbcL terminator as expression elements. The Dunaliella salina is transformed through a gene gun method or electrization method and then subjected to two-step screening to realize homogenization, fixed point integration of an exogenous gene in a Dunaliella salina chloroplast genome is ensured, and finally the chloroplast transformation Dunaliella salina strain for stably expressing the exogenous gene is obtained. In the invention, the chlN, chlB or chlL gene is selected as the homological segment and can be used as an auxiliary screening marker of a transformant; and the chlorampenicol resistant CAT gene and the resistance gene bar of the PPT are selected to be used as screening marker genes together, thus the stable transformation strain can be obtained within shorter time.

The present invention relates to a cosmetic composition for improving skin complexion comprising an alga extract. More particularly, the cosmetic composition according to the present invention comprises an extract from the alga Dunaliella Salina to provide energy to skin cells and promotes turn-over of epidermal cells, thereby providing skin complexion and clearness improving effect on the aged skin or darkened skin caused by outer circumstance and stress.

The invention discloses steelhead adult fish feed. The steelhead adult fish feed is characterized by comprising, by weight, 36-40 parts of fish meal, 6-8 parts of flour, 4-7 parts of shrimp meal, 0.9-1.5 parts of wheat shorts for fishes, 3-5 parts of chicken powder, 7-9 parts of beer yeast, 6-8 parts of extruded soybean, 3-5 parts of phospholipids powder, 20-24 parts of fish oil, 4-6 parts of soybean oil, 4-5 parts of cassava starch, 0.5-0.9 part of vitamin C, 0.25-0.4 part of complex vitamin B, 0.4-0.8 part of vitamin E, 0.03-0.15 part of dunaliella salina powder, 0.04-0.15 part of haematococcus pluvialis powder, 1-2.5 parts of choline chloride, 0.5-0.8 part of L-lysine, 1-1.5 parts of monocalcium phosphate and 0.2-0.3 part of zinc. By means of the feed, the requirement of steelhead for nutrition can be met, the steelhead immunity is improved, energy needed by growth and the development of the steelhead is provided, and the steelhead adult fish feed is suitable for application and popularization.

A process for dewatering an aqueous suspension of microalgae is disclosed in which the aqueous suspension of the algae is introduced into a bubble column or a modified bubble column for generating a froth of bubbles and adsorbed algal cells that can be separated from the aqueous suspension. In one advantageous embodiment, the bubble column is a multi-stage loop-flow flotation column that has three loop-flow zones, each of which is defined by a draft tube concentrically mounted in the column to divide each loop-flow zone into a riser and the downcomer. Fine bubbles of gas and brine are in cocurrent upward flow in the riser and in cocurrent downward flow the downcomer. A higher gas holdup is promoted in the riser than in the downcomer, thereby circulating the brine in loop-flow upwardly through the riser and downwardly through the downcomer. Liquid communication between adjacent loop-flow zones is substantially eliminated. A froth enriched in algae is generated that can be separated from the aqueous suspension. The process has application in the recovery of mixed carotenoids from Dunaliella salina.

The invention discloses a functional compound feed special for turbot. The functional compound feed consists of the following components: Peru fish meal, shrimp shell meal, a beer yeast, corn protein, fermented peanut meal powder, bread powder, calcium dihydrogen phosphate, premixed materials for fish, probiotics, imported fish oil, wheat germs, whey powder and xanthan gum, wherein the probiotics consist of freeze-dried powder of saccharomycetes, bacillus subtilis, tea polysaccharide, dunaliella salina powder, sargassum thunbergii powder and chlorella vulgaris powder in proportions. According to the turbot fed by the feed disclosed by the invention, main delicate flavor materials in muscles can be significantly increased, and the total contents of amino acids, total contents of essential amino-acids, total contents of unsaturated fatty acids and the like are improved to different extents; all indexes such as muscle strength, freshness, fishy smell and taste can be significantly improved; and the immunities of juvenile fish and adult fish can be significantly improved, so that red mouth diseases and albinism of the turbot can be effectively prevented and treated. The feed disclosed by the invention is environmentally-friendly and pollution-free in raw material, and is suitable for large-scale cultivation of the turbot.

A process for culturing the extremely halophilous green Du's algae used for health-care, medicinal and gene engineering purposes includes preparing culture liquid, regulating sanility and pH value, disinfecting, removing unuse algae, preparing alga seeds, inoculating, and controlling the environment of large-area culturing.

The invention relates to a dunaliella salina, lutein and huckleberry composition and a preparation method thereof. The composition is prepared from dunaliella salina extractive, lutein, huckleberry extractive, beewax and the balance of grape seed oil, and has remarkable antioxidation and the function of relieving visual fatigue. The invention also provides a soft capsule preparation containing the dunaliella salina, lutein and huckleberry composition and a preparation method of the soft capsule preparation.

The present invention discloses a dunaliella salina EPSP synthetase gene, a recombinant plasmid, an EPSP synzyme, and the preparation method and the purpose thereof. The dunaliella salina EPSP synthetase gene is derived from a dunaliella salina, the prepared dunaliella salina EPSP synthetase gene is cloned to an express vector, to form the recombinant plasmid which is expressed to form the EPSP synthetase. The prepared recombinant plasmid I is transferred into agrobacterium tumefaciens (Agrobacterium tumefaciens) EHA105, to be used to convert a model plant (a wild type), thus a transgenic plant which can produce the stand up quality to glyphosate can be cultivated. The present invention provides novel enzyme in order to prevent the agronomic crop from endangering by the glyphosate, thereby being advantageous to promote the yield increase and the development of the agriculture.