69 results about "Isochrysis galbana" patented technology

The invention aims at providing a novel angiotensin-converting enzyme inhibition peptide, namely an ACE inhibition peptide prepared form isochrysis galbana, and with the amino acid sequence of SEQ ID NO:1. The invention provides a preparation method of a high-activity angiotensin-converting enzyme inhibition peptide sourcing from marine microalgae. The current angiotensin-converting enzyme inhibiter mainly comes from food and large macroalgae of laver and the like, and coming from bait microalgae is not reported. The structure of the obtained active peptide with a unique structure is Tyr-Met-Gly-Leu-Asp-Leu-Lys. The structure is novel and has the characteristics of being small in molecular weight and easily absorbed by a human body.

The invention relates to a high-density culture process of autotrophic oil-producing microalgae. A fermentation method combining a partition method and a two-step method is adopted, CO2 waste gas discharged from a thermal power plant is used as a carbon source, municipal sewage after living contaminant or miscellaneous algae filtering removal is used as a culture medium, the autotrophic oil-producing microalgae is cultured in a high-density way for growth, perfectly, sea salt is added into the municipal sewage for simulating sea water so that the permeation pressure is increased, the living contaminant or miscellaneous algae pollution is prevented, and the autotrophic oil-producing microalgae is selected from one kind or several kinds of materials from autotrophic chlorella, nannochloropsis salina, botryococcus braunii, chaetoceros gracile, marine green algae, isochrysis galbana, nitzschia closterium, phaeodactylum tricornutum, dunaliella and prism-shaped algae. When the high-density culture process is adopted, high removal efficiency of nitrogen, phosphorus and CO2 can be realized, in addition, the algae concentration, the grease content and the protein content are high, the double effects of environment protection and biological resources production are reached, the operation is simple, convenient and fast, the cost is low, no pollution is caused, social and economic integrated benefits are high, and the high-density culture process is suitable for large-scale popularization and application.

The invention relates to a method for inducing the spawning of the parent Solen grandis and collecting fertilized eggs. The method comprises the following steps: the parent Solen grandis which has smooth surface of shells, no mechanical damage on feet and appearance, healthy body and high activity and also has the 8-11cm length of shells is collected to perform maturation and temporary cultivation in an induced spawning pond; when the meat condition of the parent Solen grandis is up to more than 85% and the genital gland is dissected to find that sperms/eggs can flow by gravity, the induced spawning can be performed, the liquid Isochrysis galbana extract is used to cover the entire bottom surface of the pond in the induced spawning process to stimulate the parent Solen grandis for 20-25min; when spawning is performed and the density of fertilized eggs in the induced spawning pond is up to 10-15 per milliliter, and the siphon principle is utilized to fast move the fertilized eggs to a culture pond to culture. The method of the invention has the advantages that the utilization rate of the parent Solen grandis is high, the induced spawning effect is good, the fertilized eggs are convenient and fast to collect, the fertilization rate is up to 72%, the breeding survival rate is high, etc. Only a small number of parent oysters can be used so as to obtain enough fertilized eggs for artificial breeding, thus the method has good application prospect.

The invention discloses microalgae composite living bait for shellfish breeding and a production method of microalgae composite living bait. The microalgae composite living bait is prepared by cultivating the following microalgae species to obtain a mixed microalgae solution according to the proportion of cell numbers: 15-20% of chaetoceros muelleri, 20-25% of coccolithophores, 5-10% of isochrysis galbana, 15-20% of haematococcus pluvialis, 25-30% of chlorella pyrenoidosa and 10-15% of platymanas subcordiformis, wherein the total density is (1*105) to (5*105) cells/mL. The production method comprises the following steps: (1) utilizing photobioreactors to cultivate the microalgae species respectively, thereby obtaining the microalgae solution of which the cultivation density is (1*109) to (5*1010) cells/mL; (2) preparing the microalgae solution into the microalgae composite living bait according to the proportion of cell numbers, wherein the total density of the microalgae composite living bait is (1*105) to (5*105) cells/mL. The microalgae composite living bait is prepared from various microalgae cells, so that a nutrition balance effect is realized, the survival rate of juveniles shellfish can be effectively improved, and the morbidity of the juveniles shellfish can be reduced.

The invention discloses a culture method of microalgae with high grease content. The culture method is characterized by comprising the following steps that isochrysis galbana is selected as a culturing body and put in a uniform electric field environment of which the electric field intensity ranges from 1*10<6> V/m to 2*10<6> V/m for 10 min to 20 min, then the isochrysis galbana is put in a uniform magnetic field environment of which the magnetic induction intensity ranges from 50 mT to 100 mT for 15 min to 20 min, and then culture is conducted according to the following parameters that the concentration of glucose carbon source ranges from 4 g/L to 6 g/L, the temperature ranges from 19 DEG C to 26 DEG C, the salinity ranges from 33 to 34, the pH value ranges from 8.2 to 8.4 and the illumination intensity ranges from 3,000 lux to 3,500 lux; continuous aeration is conducted during the culture period, continuous culture is conducted for 10 d to 14 d, and the microalgae with the high grease content can be obtained. The culture method is low in cost, easy and convenient to operate and capable of obtaining the microalgae with the relative high grease content.

The invention belongs to the technical field of algae and discloses a method of increasing the biomass and DHA productivity of isochrysis galbana parke. The method includes: S1) inoculating the isochrysis galbana parke, which has been cultured to logarithmic phase, on a seed pot filled with a seed culture medium, and culturing the isochrysis galbana parke for 48 h and collecting a seed liquid; S2)inoculating the seed liquid on a reaction tank filled with a fermenting culture liquid, performing fermenting culture at 22-25 DEG C, when the cultivation reaches 48 h, adding arachidonic acid and propyl gallate to the fermenting culture liquid and continuously culturing the seed liquid for 72-96 h through fermentation, and collecting algae cells. The method can increase the biomass and DHA productivity of the isochrysis galbana parke.

The invention discloses a method for purifying indoor vitiated air and belongs to the field of environment protection. According to the technical principle, indoor vitiated air flows through three kinds of marine microalgae nutrient solutions, wherein CO2, NH4, H2S and other harmful ingredients serve as nutrient matter for growth of marine microalgae to be absorbed and utilized, a great amount of oxygen is further released in the marine microalgae growth process, and finally discharged gas is fresh, clean, environmentally friendly and beneficial to human health. In addition, the cultivated halophytic chlorella, pyramidomonas sp and isochrysis galbana parke are important bait of ocean economic animal adults and larvae, such as sea urchins, sea cucumbers, scallops, abalones, mussels and prawns, and two purposes are achieved through the method. It is indicated by researches and experiments that the method for purifying indoor vitiated air is low in cost, easy to implement, easy to master and control, simple in technological processes and low in operating cost and achieves the low-carton, environment-friendly and energy-saving functions.

The invention relates to method for cultivating Isochrysis galbana Parke H29 rich in DHA outdoor in large area. The method is characterized preparing culture fluid, conserving seeds, determining culture conditions, training with salt, installing automatic temperature controlling system, expanding seeds gradually, culturing in the outdoor cultivating pool, harvesting, drying, and inspecting the final product. The superiorities of the invention are low materials and energy consumption, high production efficiency, low cost, high achievement ratio, short cultivation period, and industrialized production.

The invention provides a mixing fermentation method of chrysophyceae and photosynthetic bacteria, which belongs to the field of biotechnology. The invention concretely provides the mixing fermentation method of isochrysis galbana and ectothiorhodospira shaposhnikovii, isochrysis galbana and ectothiorhodospira shaposhnikovii are respectively subjected to seed activation, culture, and then mixing fermentation in an illuminating fermentation tank, so that isochrysis galbana content is greatly increased which is 5-10 times of that through normal culture.

The invention discloses an isochrysis galbana 3010 culture medium composition. The isochrysis galbana 3010 culture medium composition comprises the following components: 75mg/L of NaNO3, 5mg/L of NaH2PO4, 3.16mg/L of FeCl3.6H2O, 4.36mg/L of Na2EDTA, 0.23g/L of ZnSO4.7H2O, 0.18g/L of MnCl2.4H2O, 0.01g/L of CuSO4.5H2O, 0.007g/L of Na2MoO4.2H2O, 0.12g/L of CoCl2.6H2O, an additive prepared from glucose and glycine, and a plant growth regulator prepared from gibberellins and 6-BA. The isochrysis galbana 3010 culture medium composition prepared with the technical scheme is adopted for performing culture discovery on isochrysis galbana 3010; and the isochrysis galbana 3010 has the characteristics of high growth rate and high cell density.

The invention discloses a compound Sargassum, fruit and vegetable beverage capable of stimulating children's intelligence development and a preparation method of the compound Sargassum, fruit and vegetable beverage and belongs to the field of beverage production. The compound Sargassum, fruit and vegetable beverage is prepared from Dunaliella Salina powder, Isochrysis Galbana powder, Nannochloropsis Oculata powder, scallop juice, Concha Ostreae juice, grape juice, pineapple juice, kiwi-fruit juice, tomato juice, orange juice, Spinacia Oleracea juice, carrot juice and distilled water according to a scientific technology. The compound Sargassum, fruit and vegetable beverage capable of stimulating children's intelligence development is scientific and reasonable in formula, available to raw materials, simple in technology, low in cost, green, natural, unique and harmonized in flavor, mild in taste, free from foreign smell, clear, transparent, cool, refreshing, good in fluidity and natural in faint scent, and is capable of tonifying the spleen, reinforcing the stomach, nourishing the heart, moistening the lung and nourishing the liver and kidney, thereby being suitable for people of all ages and particularly helping stimulate brain development and enhance intelligence of children.

The invention provides a strain of Alteromonas and application thereof. The strain is obtained from the algae liquid of Isochrysis sphaeroides and can promote the growth of Isochrysis sphaeroides. Thestrain of Alteromonas provided by the present invention is named Alteromonas (Alteromonas sp.), the strain number is NBU-A-0001, and the preservation number is CCTCC M20200010, and the preservation date is January 3, 2020. The preservation unit is the China Type Culture Collection, and the address is Wuhan University, Wuhan. The strain of Alteromonas screened and obtained in the present inventioncan promote the growth of Isochrysis globosum, thereby effectively expanding the output of Isochrysis globosum and meeting the requirement of Isochrysis globosus as a feed.

The invention discloses a method for performing outdoor culture on isochrysis galbana parke suitable for southern climatic conditions. According to the method, an outdoor white plastic bucket is adopted for performing sealed aerated culture, and the culture appliance comprises the white plastic bucket, a transparent plastic cover covering the opening of the white plastic bucket, an air pipe, an air stone and a pumping device. The method is simple, feasible, low in requirement on culture conditions, low in culture cost and suitable for outdoor large-scale cultivation suitable for China southern climatic conditions, and the requirements of China southern marine economic shellfishes on important initial feed isochrysis galbana parke in breeding seasons are met.

The invention relates to an artificial culture method for Onchidium struma larvae. The method comprises the following steps of: disinfecting a larva incubator and a culture box; disinfecting sponge bars; preparing disinfecting seawater; pouring the disinfecting seawater into the larva incubator and the culture box, aerating the disinfecting seawater in the larva incubator and the culture box by using an aerator, and controlling the water temperature and illumination intensity in the larva incubator and the culture box; putting fertilizer eggs of Onchidium struma into the larva incubator, and when checking to discover that Onchidium struma larvae are out of membranes in the larva incubator, putting the disinfected sponge bars; and squeezing the sponge bars to make water squeezed out of thesponge bars flow into water in the culture box together with the Onchidium struma larvae, and feeding the Onchidium struma larvae with chlorella vulgaris, isochrysis galbana and chaetoceros mulleri after the Onchidium struma larvae are transferred from the larva incubator into the culture box. The survival rate of the artificially cultured Onchidium struma larvae is 77.0-86.5 percent, and a requirement for large-scale production is met.

The invention discloses a method for producing a laying hen feed additive by efficiently utilizing orange peel and shaddock peel. The method comprises the steps that firstly, raw materials which comprise, by weight, 15-17 parts of orange peel powder, 20-22 parts of soybean dreg powder, 24-26 parts of shaddock peel powder, 4-6 parts of camellia seed powder, 5.6 parts of isochrysis galbana powder and 9 parts of auxiliaries are prepared, wherein the auxiliaries comprise montmorillonite, L-leucine and chitosan according to the ratio of 1.7:1.1:1.6; secondly, all raw material coarse powder is mixed and then evenly mixed with the auxiliaries; thirdly, the mixture is processed with a high-energy vibrating mill for 15-30 min, the high-energy vibrating mill is halted for 2.5 min, then the mixture is processed for 12-13 min, two times of different solid-state mechanical chemical reactions take place in all the raw materials, screening is performed with a 400-mesh screen, and the finished product is obtained. According to the method, carotenoid components in the shaddock peel and the orange peel can be efficiently released, and the bitter taste is significantly reduced; by using the laying hen feed additive, the color of egg yolk is obviously increased, economic benefit can be brought to enterprises, and the environmental protection pressure can be relieved.

The invention belongs to the technical field of algae, and discloses a method for reducing isochrysis-galbana adherence and increasing the growth amount. The method includes the following steps that an isochrysis-galbana seed solution is inoculated to a reaction pool containing a culture solution according to the inoculation amount of 5% to 10%, then 100 mg/L-200 mg/L amino acid is added, and themixture is cultured at the temperature of 20 DEG C to 22 DEG C, wherein the light-dark ratio is 12:12, and the ventilation rate is 0.4 vvm to 0.5 vvm; culturing is carried out for 48 h to 72 h, and algal cells are collected. By means of the method, the isochrysis-galbana adherence phenomenon can be obviously reduced, and the biomass of the algal cells is increased.

The invention discloses an artificial breeding feed for sipunculus nudus. The feed comprises the following raw materials in parts by weight: 21-25 parts of flour, 10-13 parts of soybean meal, 7-9 parts of corn meal, 5-10 parts of cuttlefish powder, 8-12 parts of silkworm chrysalis powder, 4-6 parts of shrimp shell powder, 3-8 parts of liver powder, 25-28 parts of isochrysis galbana, 17-22 parts ofchaetoceros, 15-19 parts of cryptocoryne parva, 2-9 parts of riccia fluitans, 1-6 parts of needle spikesedge herb, 11-15 parts of calcium dihydrogen phosphate, 7-13 parts of zeolite powder, 1-4 partsof montmorillonite, 1-4 parts of bentonite, 3-6 parts of choline chloride, 5-7 parts of betaine, 16-22 parts of sodium humate, 6-10 parts of allicin and 7-9 parts of complex minerals. The feed can improve the survival rate of artificially bred sipunculus nudus, shorten the breeding time of the sipunculus nudus, and improve the yield of the sipunculus nudus.