Artificial culture method for Onchidium struma larvae

A kind of technology of artificial cultivation of Tubo sulfonic acid, applied in fish farming, application, climate change adaptation and other directions, can solve problems such as habitat destruction of Tubo sulfonic acid, and achieve the effect of improving the cultivation density and survival rate.

Inactive Publication Date: 2012-07-04
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the intensification of human development activities, the habitat of Sulphuria spp. has been severely damage...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The method for artificially cultivating the larvae of the sulphurus tumefii comprises the following steps:

[0026] a. Soak the incubator and incubator in 100mg / L KMnO 4 Disinfect in the solution for 1 hour; Prepare the sponge strip with pore diameter of 1.0mm, and disinfect the sponge strip with 1mg / L iodine seawater solution for 5 minutes; Dissolve solid iodine in natural seawater with a salinity of 16 to obtain an iodine content of 1~ 2mg / L of sterilized natural seawater;

[0027] b. Pour the sterilized natural seawater into the incubator and the incubator, and use an aerator to increase oxygen in the sterilized natural seawater in the incubator and the incubator, so that the dissolved oxygen of the sterilized natural seawater in the incubator and the incubator is ≥ 5mg / L, the temperature of the water body in the incubator and incubator is controlled at 28~30°C, and the light intensity in the incubator and incubator is controlled at 500~1000LX;

[0028] c. Put the ...

Embodiment 2

[0033] The method for artificially cultivating the larvae of the sulphurus tumefii comprises the following steps:

[0034] a. Soak the incubator and incubator in 200mg / L KMnO 4 solution for 3 hours; prepare sponge strips with a pore size of 1.5 mm, and disinfect the sponge strips with 2 mg / L iodine seawater solution for 20 minutes; prepare sterilized artificial seawater with a salinity of 25, and the specific disinfection method is: aerate 12~ 24 hours of tap water is boiled and cooled to normal temperature, and then seawater essence is added to make sterilized artificial seawater with required salinity;

[0035] b. Pour the sterilized seawater into the incubator and the incubator, and use an aerator to increase oxygen in the sterilized seawater in the incubator and the incubator, so that the dissolved oxygen in the sterilized seawater in the incubator and the incubator is ≥5mg / L, the temperature of the water body in the incubator and incubator is controlled at 28~30°C, and ...

Embodiment 3

[0041] The method for artificially cultivating the larvae of the sulphurus tumefii comprises the following steps:

[0042] a. Soak the incubator and incubator in 150mg / L KMnO 4 solution for 2 hours; prepare sponge strips with a pore size of 1.3 mm, and disinfect the sponge strips with 1.5 mg / L iodine seawater solution for 12 minutes; prepare sterilized natural seawater with a salinity of 20, and the specific disinfection method is: dissolve in natural seawater After solid iodine, sterilized natural seawater with an iodine content of 1-2 mg / L is obtained;

[0043] b. Pour the sterilized seawater into the incubator and the incubator, and use an aerator to add oxygen to the sterilized seawater in the incubator and the incubator, so that the dissolved oxygen in the sterilized seawater in the incubator and the incubator is ≥5mg / L , the temperature of the water body in the incubator and incubator is controlled at 28~30°C, and the light intensity in the incubator and incubator is co...

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Abstract

The invention relates to an artificial culture method for Onchidium struma larvae. The method comprises the following steps of: disinfecting a larva incubator and a culture box; disinfecting sponge bars; preparing disinfecting seawater; pouring the disinfecting seawater into the larva incubator and the culture box, aerating the disinfecting seawater in the larva incubator and the culture box by using an aerator, and controlling the water temperature and illumination intensity in the larva incubator and the culture box; putting fertilizer eggs of Onchidium struma into the larva incubator, and when checking to discover that Onchidium struma larvae are out of membranes in the larva incubator, putting the disinfected sponge bars; and squeezing the sponge bars to make water squeezed out of thesponge bars flow into water in the culture box together with the Onchidium struma larvae, and feeding the Onchidium struma larvae with chlorella vulgaris, isochrysis galbana and chaetoceros mulleri after the Onchidium struma larvae are transferred from the larva incubator into the culture box. The survival rate of the artificially cultured Onchidium struma larvae is 77.0-86.5 percent, and a requirement for large-scale production is met.

Description

technical field [0001] The invention relates to a method for cultivating shellfish seedlings, and in particular discloses a method for artificially cultivating sulphate larvae. Background technique [0002] Tumor back stone sulfur ( Onchidium struma ), commonly known as soil sea cucumber, also known as sea cucumber, belongs to the mollusk phylum, gastropod class, and pulmonary snail subclass. Tumorback stones inhabit the high tide zone of the sea, and are creatures in the transition zone between the sea and the land. Tuobei sulfonium has high edible value and certain medicinal value, and is a high-grade aquatic product. In addition, Lithocarpus is a precious species that needs to be protected urgently in the coastal biome. It is closely related to the survival of other species, and it is also an indicator organism for a good ecological environment. Due to the intensification of human development activities, the habitat of Sulphuria spp. has been severely damaged, and its ...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 黄金田沈永龙杨文平刘飞
Owner YANCHENG INST OF TECH
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