11 results about "Chlorella" patented technology

The application discloses a kind of long-acting targeted cultivation alga high-density complex of water body and preparation method thereof, it is related to aquaculture field, the high-density complex includes the following weight parts components: small material premix 3-5 parts, sodium chloride 35-40 parts, gelatinized starch 1-3 parts, attapulgite 40-50 parts, cement 3-7 parts, titanate coupling agent 1-3 parts, molasses 2-4 parts and purified water 1-5 parts.The preparation method of the high-density complex includes the following steps: small material premix is subpackaged, is stirred uniformly with other components and adds molasses to carry out humidity control, then subpackaging and compression molding are formed, in turn plastic envelope, package and film winding.The long-acting targeted cultivation alga high-density complex of water body provided in the application can directional cultivation diatom and chlorella, and continuously release functional material, stabilize the abundance of beneficial algae, effectively solve the problem that directional cultivation beneficial organism cannot be carried out and functional material release speed is too fast.

The method for preparing high-phosphorus Chlamydomonas based on PTB2 overexpression and its application in phosphorus recovery belongs to the fields of bioengineering and environmental science. This method obtains recombinant Chlamydomonas by cloning and overexpressing the PTB2 gene, a sodium-phosphorus-dependent phosphate transporter, from Chlamydomonas reinhardtii. This recombinant Chlamydomonas exhibits a synergistic and significant increase in total phosphorus content and polyphosphate content, while also showing promoted growth. The high-phosphorus recombinant Chlamydomonas of this invention can efficiently remove phosphorus from water bodies and can be used for eutrophication control; simultaneously, its high-phosphorus biomass can serve as a high-quality microalgal fertilizer raw material, providing an efficient and environmentally friendly technical solution for the biological recovery and recycling of phosphorus resources.

Biostimulant compositions and methods for enhancing soil organic carbon are provided. A mixture of bioavailable B12 and amino acids phenylalanine (Phe) and lysine (Lys)—was found to enhance soil organic carbon and crop growth due to synergistic effects between the bioavailable B12 and the PheLys. The bioavailable B12 and the PheLys may be derived from various sources, including, e.g., cyanobacteria (e.g., Spirulina—Arthrospira platensis) or green algae (e.g., Nannochloropsis spp.) cultivated under photosynthetically controlled conditions to yield upregulated bio-active compounds such as bioavailable B12 and amino acids. One or more sources may be used to provide the bioavailable B12, Phe and Lys, and algal cultivation may be integrated with additional industries to yield synergistic biostimulation effects.

The application relates to a preparation method and application of an algae-loaded nano zero-valent iron composite material, and the process steps of the method are as follows: taking chlorella as a carrier, adding sodium borohydride and ferric chloride in proportion and in sequence, gently shaking to start a reaction, fully combining nano zero-valent iron and algal cells, and obtaining a suspension; carrying out solid-liquid separation on the suspension, and obtaining the algae-loaded nano zero-valent iron material. The algae-loaded nano zero-valent iron material prepared by the method can fully utilize the dispersibility of algae to reduce the agglomeration of nano zero-valent iron, and has a remarkable effect when treating arsenic-containing wastewater.

The invention discloses a fungal inoculant and application thereof in algae crust, and belongs to the field of microorganisms. The technical problem to be solved by the invention is how to construct the algae crust. The application of the fungal inoculant in the algal crust comprises the following steps: inoculating deep-color endophytic fungi and microalgae to the surface layer of soil to be remediated, and culturing to realize the construction of the algal crust; the deep-color endophytic fungi are bacterial strains with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.17463 in the China General Microbiological Culture Collection Center; the microalgae is composed of Microcoleus sp., Scenedesmus sp. And Chlorella sp., and the microcoleus sp., the Scenedesmus sp. And the Chlorella sp. Are used in the method. According to the method, rapid formation of algae crust and improvement of soil carbon accumulation are successfully realized, the key technical problems of long crust period, poor adaptability and the like are solved, and the purpose of ecological restoration of degraded soil in an arid and semi-arid mining area is favorably realized.

This invention relates to the field of resource and environmental technology, specifically to a method and application of an algae-bacteria hybrid system for treating high ammonia nitrogen wastewater. By regulating nutrient and operational parameters, a functional community is constructed and maintained, dominated by microalgae and supplemented by bacteria and cyanobacteria, with their relative abundance within a preset optimized range. A clear interspecific interaction network forms within the system: microalgae such as *Nostoc* and *Chlorella* exhibit mutualistic symbiosis, while bacteria such as *Flavobacterium* show antagonistic relationships with these microalgae. Positive and negative feedback mechanisms maintain community balance and system robustness. The three components complement each other: microalgae primarily drive photocatalytic nitrogen and phosphorus removal, cyanobacteria provide nitrogen fixation and stability, and functional bacteria participate in organic matter degradation and nitrogen cycling, achieving synergistic metabolism through material exchange. Microbial ecological analysis confirms that a stable community structure and internal interaction mechanisms are the core of efficient and stable ammonia nitrogen reduction. This technology offers precise regulation, stable operation, and is environmentally friendly and economical, and can be widely applied to the treatment of various types of ammonia nitrogen-polluted water bodies.

The invention discloses an efficient microalgae culture system and method based on a light guide fabric assembly, and belongs to the technical field of microalgae culture, the efficient microalgae culture system comprises a reactor, the light guide fabric assembly is arranged in the reactor, and the light guide fabric assembly efficiently conducts an external light source and uniformly scatters the light source into the reactor; the reactor is provided with a light guide fabric assembly, the surface of the light guide fabric assembly is provided with a microporous structure for fixing and attaching microalgae, the light guide fabric assembly is connected with a light control unit, and the reactor is also provided with a gas control unit for providing carbon-rich air, a liquid control unit for circulating a culture medium and a temperature control and nutrition control unit for providing proper temperature and blending the culture medium. The invention provides a universal platform, and by embedding optimized process parameters for different algae species (such as chlorella and tribonema), upgrading from'universal culture 'to'precise culture' is realized, and the yield of a target product can be effectively and directionally improved.

The present application relates to a kind of in sequence batch reactor using chlorella short-range nitrification denitrification coupling anaerobic ammonia oxidation sludge advanced denitrification method.Microalgae use the carbon dioxide dissolved in water or released by bacterial respiration to produce oxygen for bacteria by photosynthesis under illumination, and bacteria provide metabolites and inorganic carbon source for the growth of microalgae, resulting in most of the ventilation energy saving of algal-bacterial symbiotic system. Short-range nitrification denitrification bacteria and anaerobic ammonia oxidation bacteria achieve efficient removal of nitrogen. Chlorella in microalgae can withstand high ammonia nitrogen, high salt and low temperature, therefore, the process of starting and maintaining dominant algae, short-range nitrification denitrification bacteria and anaerobic ammonia oxidation bacteria community combination has great potential in high ammonia nitrogen wastewater treatment. The present application selects SBR as the reactor, controls pH, DO and records according to preset time, and illuminates the reactor by light incubator, and the removal rates of ammonia nitrogen and total nitrogen reach 99.76% and 98.08% respectively.

The invention discloses a microalgae carbon sequestration system and method, and belongs to the field of carbon dioxide capture and utilization. The system comprises a natural pond, a gas supply unit, a photobioreactor, an MBR separation tank and the like, and stable operation is guaranteed through PLC automatic control and pipeline electric heat tracing. According to the method, wild type marine nannochloropsis oculata is taken as a material, CO2 gradient domestication is carried out, variable light intensity, bacteria-algae synergism and precise nutrition addition culture are combined, and then separation, concentration, dehydration and algal fat extraction are carried out to prepare the biodiesel. According to the method, the cost is reduced and the water body is purified by utilizing a natural pond water source, the microalgae carbon sequestration efficiency and biomass are remarkably improved, CO2 in a steel mill is converted into high-value biodiesel, and a low-cost and sustainable scheme is provided for carbon neutrality.

The invention involves mutant or recombinant Chlorophyte algal organisms that have a genetic modification in a gene encoding a chloroplastic signal recognition particle 43 (cpSRP43). In one embodiment the Chlorophyte organisms are Trebouxiophyte algae that are diploid or polyploid for a gene encoding a chloroplastic signal recognition particle 43 (cpSRP43). The mutant organisms can have a genetic modification in one allele of the gene but not in another allele of the gene. The mutant or algal organisms have higher biomass and lipid productivity. Additional mutant or algal organisms are disclosed that also have a genetic modification to one or more genes encoding a light harvesting chlorophyll a / b (binding) protein.

The application discloses a kind of fungal inoculant and its application in algae crust, belong to the field of microorganism.The technical problem solved by the application is how to construct algae crust.The application of fungal inoculant disclosed in the application in algae crust comprises the following steps: after dark endophytic fungi and microalgae are inoculated to the surface layer of soil to be repaired, culture is carried out, the construction of algae crust is realized;the dark endophytic fungi are the strain with the preservation number CGMCC No.17463 in China General Microbiological Culture Collection Center;the microalgae are composed of Microcoleus sp., Scenedesmus sp.and Chlorella sp.The application successfully realizes the rapid formation of algae crust, improves soil carbon accumulation, solves the key technical problems such as long crust period and poor adaptability, and is beneficial to the purpose of ecological restoration of degraded soil in arid and semiarid mining areas.