29 results about "Chlorella" patented technology

The invention discloses engineering chlamydomonas reinhardtii for synthesizing high-value pigment and a construction method and application thereof.An engineering chlamydomonas reinhardtii strain Blup-BKT-CHYB-CHLC is preserved in the China General Microbiological Culture Collection Center on September 1, 2025, and the preservation number is CGMCC NO.46634. The content of astaxanthin in the constructed engineering chlamydomonas reinhardtii strain reaches 25.5 mg / L, and the content of astaxanthin in the engineering chlamydomonas reinhardtii strain reaches 9.5 mg / L; in addition, the light energy utilization efficiency is remarkably enhanced, the stress resistance is improved, very strong oxidation resistance is achieved, the accumulation of astaxanthin endows the engineering strain with highlight tolerance, the yield of high-value pigments is increased, and the biomass of the engineering strain is greatly superior to that of a wild strain under the highlight condition. In addition, the chlorophyll c synthase is applied to the reconstruction of a pigment system of the chlamydomonas reinhardtii for the first time, a frontier tool is quickly converted, and a molecular mechanism of'chlorophyll c-astaxanthin-blue pigment 'for synergistically regulating the photosynthetic efficiency of the chlamydomonas reinhardtii and product synthesis is clarified for the first time. The research blank of pigment system coupling regulation and control of photosynthesis and metabolism in the chlamydomonas reinhardtii is filled.

The invention discloses a system for removing ammonia gas and hydrogen sulfide in water-soaked manure sewage, and relates to the technical field of pig farm sewage deodorization treatment. The invention discloses a system for removing ammonia gas and hydrogen sulfide in water-soaked manure sewage. The system comprises an air-water ratio self-absorption system, a photosynthetic oxygen self-production system and a double-sided binary deodorization system, wherein the air-water ratio self-absorption system is built above a sewage storage tank; and the photosynthetic oxygen self-production system and the double-sided binary deodorization system are built in the air-water ratio self-absorption system. The invention develops a system for removing ammonia gas and hydrogen sulfide in water-soaked manure sewage, and the system is based on the characteristics of sewage and the dissolution and discharge rule of odor, combines a coating type photocatalyst and negative oxygen ion deodorization technology, integrates the photosynthetic oxygen production capacity of chlorella, and considers the factors of illumination and non-illumination, so that the water-soaked manure sewage treatment efficiency is improved, and the water-soaked manure sewage treatment efficiency is improved. According to the system disclosed by the invention, the interaction relationship among all factors is systematically researched, so that the effects of quickly removing and improving the deodorization efficiency can be achieved, and the framework structure constructed by the system is low in cost, easy to carry and reusable.

The invention relates to a device and a method for producing a microalgae liquid fertilizer by utilizing monosodium glutamate wastewater, and belongs to the technical field of microalgae fertilizer production devices and methods. Comprising a dilution device, a buffer pool and a pipeline type photobioreactor which are sequentially communicated through a pipeline, a culture medium inlet and a monosodium glutamate wastewater inlet are formed in the dilution device, and a chlorophyll luminoscope, an ultraviolet visible light detector and a water quality tester are installed on an output pipeline of the pipeline type photobioreactor. And the operation of the buffer pool, the tube (channel) type photobioreactor, the chlorophyll luminoscope, the ultraviolet visible light detector and the water quality tester is controlled by the console. According to the method, a culture medium suitable for microalgae growth is prepared by diluting monosodium glutamate wastewater in a certain proportion and adding necessary nutrient elements, chlorella capable of rapidly proliferating in the monosodium glutamate wastewater is cultured by using a photobioreactor, and the chlorella whole-cell sap can promote wheat seedling growth and rooting through tests.

The invention discloses a modified red mud-epoxy resin composite coating as well as a preparation method and application thereof. The composite coating is prepared by mixing modified red mud serving as a functional filler with epoxy resin and a curing agent, the composite coating is applied to the surface of Q235 carbon steel as a bifunctional corrosion-resistant and biological fouling-resistant composite coating, and shows remarkable corrosion resistance and biological fouling resistance; the impedance radiuses of the composite material can respectively reach 8.37 * 10 < 9 > omega.cm < 2 > and 2.69 * 10 < 6 > omega.cm < 2 > after the composite material is soaked in a 3.5 wt% NaCl solution for 1 day and 60 days; besides, the red mud / epoxy resin composite coating generates an active substance (. O2 <->) through a photo-induced electron-hole pair and a redox reaction, so that growth of bacteria (such as escherichia coli and staphylococcus aureus) and algae (chlorella) can be effectively inhibited, and excellent biological fouling resistance is shown.

The application discloses a kind of long-acting targeted cultivation alga high-density complex of water body and preparation method thereof, it is related to aquaculture field, the high-density complex includes the following weight parts components: small material premix 3-5 parts, sodium chloride 35-40 parts, gelatinized starch 1-3 parts, attapulgite 40-50 parts, cement 3-7 parts, titanate coupling agent 1-3 parts, molasses 2-4 parts and purified water 1-5 parts.The preparation method of the high-density complex includes the following steps: small material premix is subpackaged, is stirred uniformly with other components and adds molasses to carry out humidity control, then subpackaging and compression molding are formed, in turn plastic envelope, package and film winding.The long-acting targeted cultivation alga high-density complex of water body provided in the application can directional cultivation diatom and chlorella, and continuously release functional material, stabilize the abundance of beneficial algae, effectively solve the problem that directional cultivation beneficial organism cannot be carried out and functional material release speed is too fast.

This invention belongs to the field of biotechnology and relates to a high-concentration carbon dioxide-tolerant *Synechococcus* species, its preparation method, and its applications. The high-concentration carbon dioxide-tolerant *Synechococcus* species includes a CRISPR / dCas9 plasmid, a gRNA plasmid, and a knockout ω plasmid. This invention enables the growth of PCC7942 under high CO2 conditions by transcribedly activating PEPC using the CRISPR / dCas9 system. Furthermore, the fusion between the ω subunit of RNAP and a Cas9 nuclease mutant that binds to the upstream promoter region allows for programmable transcriptional activation. By knocking out the ω subunit, the CRISPR / dCas9 transcriptional regulation system is used to optimize the microalgal photosynthetic carbon fixation pathway, thereby improving the growth and carbon fixation efficiency of microalgae under high CO2 conditions.

The invention discloses a method for producing chrysophyceae laminarin through high-density culture of chrysophyceae and an application of the chrysophyceae laminarin. The culture method comprises the following steps: (1) culturing a chrysophyceae species under low-temperature and low-light conditions, and then transferring the chrysophyceae species into a seed culture medium for culturing to obtain activated chrysophyceae species; (2) placing the activated algae species obtained in the step (1) under a dark condition for shake culture to obtain dark adaptation algae species; and (3) inoculating the dark adaptation algae seeds into a fermentation tank, and adding a basic culture medium and a fed-batch culture medium for fermentation culture. By selecting the nitrogen source and accurately controlling the ammonium concentration, the double improvement of the biomass and the yield of the chrysophyceae laminarin is realized, the adverse effect of the high-concentration nitrogen source on growth is avoided, and the culture efficiency and the product economy are improved.

The method for preparing high-phosphorus Chlamydomonas based on PTB2 overexpression and its application in phosphorus recovery belongs to the fields of bioengineering and environmental science. This method obtains recombinant Chlamydomonas by cloning and overexpressing the PTB2 gene, a sodium-phosphorus-dependent phosphate transporter, from Chlamydomonas reinhardtii. This recombinant Chlamydomonas exhibits a synergistic and significant increase in total phosphorus content and polyphosphate content, while also showing promoted growth. The high-phosphorus recombinant Chlamydomonas of this invention can efficiently remove phosphorus from water bodies and can be used for eutrophication control; simultaneously, its high-phosphorus biomass can serve as a high-quality microalgal fertilizer raw material, providing an efficient and environmentally friendly technical solution for the biological recovery and recycling of phosphorus resources.

Biostimulant compositions and methods for enhancing soil organic carbon are provided. A mixture of bioavailable B12 and amino acids phenylalanine (Phe) and lysine (Lys)—was found to enhance soil organic carbon and crop growth due to synergistic effects between the bioavailable B12 and the PheLys. The bioavailable B12 and the PheLys may be derived from various sources, including, e.g., cyanobacteria (e.g., Spirulina—Arthrospira platensis) or green algae (e.g., Nannochloropsis spp.) cultivated under photosynthetically controlled conditions to yield upregulated bio-active compounds such as bioavailable B12 and amino acids. One or more sources may be used to provide the bioavailable B12, Phe and Lys, and algal cultivation may be integrated with additional industries to yield synergistic biostimulation effects.

The invention provides an algae biotoxicity detection device and a use method thereof.The detection device comprises a peristaltic pump, a metering unit, a multi-way valve, a chlorella solution tank, a distilled water tank, a water sample tank, a culture solution tank, a waste liquid tank, a control mechanism and a detection unit, and the detection unit comprises a shell, a culture tube A, a culture tube B and a constant-temperature control mechanism; the culture tube A and the culture tube B are respectively provided with a dissolved oxygen sensor and a light source group for providing illumination for photosynthesis of chlorella, and the culture tube A and the culture tube B are respectively connected with a multi-way valve through pipelines. According to the invention, the inhibition rate of the growth of chlorella is calculated by monitoring dissolved oxygen to realize on-line biotoxicity monitoring, and the device is simple in structure and high in detection precision.

The invention discloses a novel chlorella-loaded 3D printing photo-thermal scaffold and a preparation method and application thereof.According to the novel chlorella-loaded 3D printing photo-thermal scaffold and the preparation method and application thereof, the characteristic that the tumor tissue local anoxic microenvironment of melanoma can be improved through the efficient photosynthetic oxygen release capacity of chlorella MA, so that recurrence and metastasis of the melanoma are more effectively inhibited is utilized, and through multiple tests, the chlorella-loaded 3D printing photo-thermal scaffold has a good application prospect. Chlorella MA and polydopamine (PDA) are reasonably integrated, related parameters are optimized, and the novel chlorella-loaded 3D printing photo-thermal stent prepared by using a microfluidic technology and a biological 3D printing technology has double effects of PTT and oxygen therapy. The chlorella-carrying 3D printing photo-thermal stent provided by the invention is complete in form, can stably release oxygen and has an excellent photo-thermal effect. In-vivo and in-vitro experiments prove that the chlorella-loaded 3D printing photo-thermal scaffold has a certain tumor killing effect and shows the potential of promoting normal cell proliferation.

The invention relates to the technical field of aquatic breeding, and discloses an indoor cement pond artificial breeding method for babylonia lutosa, which comprises the following steps: sterilizing a water body with ultraviolet rays, storing oocysts of the babylonia lutosa, removing infected oocysts, and carrying out specific running water change 24 hours before hatching; after the larvae are hatched, a nutritional agent containing ethanol clostridium protein and agrocybe cylindracea polysaccharide is put every day, the larvae are fed with single-cell algae such as chrysophyceae, platymonas subcordiformis and chlorella in the early stage, and the larvae are fed with shrimp pond algae in the middle stage until the larvae are attached and During seedling culture, the seedling culture pond is continuously inflated, water dissolved oxygen, salinity, temperature, pH value and other parameters are controlled, and finally the snail seedlings are collected to end seedling culture. The artificial breeding method for babylonia lutosa in the indoor cement pool is suitable for one or more of babylonia lutosa and babylonia lutosa, and the success rate and the seedling quality of babylonia lutosa in the indoor cement pool can be effectively improved.

The application relates to a preparation method and application of an algae-loaded nano zero-valent iron composite material, and the process steps of the method are as follows: taking chlorella as a carrier, adding sodium borohydride and ferric chloride in proportion and in sequence, gently shaking to start a reaction, fully combining nano zero-valent iron and algal cells, and obtaining a suspension; carrying out solid-liquid separation on the suspension, and obtaining the algae-loaded nano zero-valent iron material. The algae-loaded nano zero-valent iron material prepared by the method can fully utilize the dispersibility of algae to reduce the agglomeration of nano zero-valent iron, and has a remarkable effect when treating arsenic-containing wastewater.

The invention discloses a fungal inoculant and application thereof in algae crust, and belongs to the field of microorganisms. The technical problem to be solved by the invention is how to construct the algae crust. The application of the fungal inoculant in the algal crust comprises the following steps: inoculating deep-color endophytic fungi and microalgae to the surface layer of soil to be remediated, and culturing to realize the construction of the algal crust; the deep-color endophytic fungi are bacterial strains with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.17463 in the China General Microbiological Culture Collection Center; the microalgae is composed of Microcoleus sp., Scenedesmus sp. And Chlorella sp., and the microcoleus sp., the Scenedesmus sp. And the Chlorella sp. Are used in the method. According to the method, rapid formation of algae crust and improvement of soil carbon accumulation are successfully realized, the key technical problems of long crust period, poor adaptability and the like are solved, and the purpose of ecological restoration of degraded soil in an arid and semi-arid mining area is favorably realized.

This invention relates to the field of resource and environmental technology, specifically to a method and application of an algae-bacteria hybrid system for treating high ammonia nitrogen wastewater. By regulating nutrient and operational parameters, a functional community is constructed and maintained, dominated by microalgae and supplemented by bacteria and cyanobacteria, with their relative abundance within a preset optimized range. A clear interspecific interaction network forms within the system: microalgae such as *Nostoc* and *Chlorella* exhibit mutualistic symbiosis, while bacteria such as *Flavobacterium* show antagonistic relationships with these microalgae. Positive and negative feedback mechanisms maintain community balance and system robustness. The three components complement each other: microalgae primarily drive photocatalytic nitrogen and phosphorus removal, cyanobacteria provide nitrogen fixation and stability, and functional bacteria participate in organic matter degradation and nitrogen cycling, achieving synergistic metabolism through material exchange. Microbial ecological analysis confirms that a stable community structure and internal interaction mechanisms are the core of efficient and stable ammonia nitrogen reduction. This technology offers precise regulation, stable operation, and is environmentally friendly and economical, and can be widely applied to the treatment of various types of ammonia nitrogen-polluted water bodies.

The invention discloses an efficient microalgae culture system and method based on a light guide fabric assembly, and belongs to the technical field of microalgae culture, the efficient microalgae culture system comprises a reactor, the light guide fabric assembly is arranged in the reactor, and the light guide fabric assembly efficiently conducts an external light source and uniformly scatters the light source into the reactor; the reactor is provided with a light guide fabric assembly, the surface of the light guide fabric assembly is provided with a microporous structure for fixing and attaching microalgae, the light guide fabric assembly is connected with a light control unit, and the reactor is also provided with a gas control unit for providing carbon-rich air, a liquid control unit for circulating a culture medium and a temperature control and nutrition control unit for providing proper temperature and blending the culture medium. The invention provides a universal platform, and by embedding optimized process parameters for different algae species (such as chlorella and tribonema), upgrading from'universal culture 'to'precise culture' is realized, and the yield of a target product can be effectively and directionally improved.

The present application relates to a kind of in sequence batch reactor using chlorella short-range nitrification denitrification coupling anaerobic ammonia oxidation sludge advanced denitrification method.Microalgae use the carbon dioxide dissolved in water or released by bacterial respiration to produce oxygen for bacteria by photosynthesis under illumination, and bacteria provide metabolites and inorganic carbon source for the growth of microalgae, resulting in most of the ventilation energy saving of algal-bacterial symbiotic system. Short-range nitrification denitrification bacteria and anaerobic ammonia oxidation bacteria achieve efficient removal of nitrogen. Chlorella in microalgae can withstand high ammonia nitrogen, high salt and low temperature, therefore, the process of starting and maintaining dominant algae, short-range nitrification denitrification bacteria and anaerobic ammonia oxidation bacteria community combination has great potential in high ammonia nitrogen wastewater treatment. The present application selects SBR as the reactor, controls pH, DO and records according to preset time, and illuminates the reactor by light incubator, and the removal rates of ammonia nitrogen and total nitrogen reach 99.76% and 98.08% respectively.

The invention discloses a method for synthesizing recombinant human defensin with antibacterial activity by using chlamydomonas and application of the recombinant human defensin, compared with a traditional expression system, the method has the advantages in the aspects of cost effectiveness, expandability and safety, and the recombinant human defensin produced by the method shows effective antibacterial activity on various pathogenic bacteria and can be used for preparing the recombinant human defensin with antibacterial activity. Under the action of different temperatures, pH values and proteases, the compound has good stability, low hemolytic activity and extremely low cytotoxicity to mammalian cells. The invention also relates to the use of the recombinant human defensin as an antibacterial agent in a variety of applications, including medicaments, disinfectants and agricultural products.

The application discloses a method for treating dairy farm wastewater based on Synechococcus, Aspergillus and green algae. In the wastewater treatment method, Aspergillus is used in the first step, and can realize rapid conversion of organic matter in dairy farm wastewater. Synechococcus is used in the second step, and has strong treatment capacity for high-concentration wastewater in a dairy farm, and is named Synechococcus ZSL-NS1. On the basis of the first two steps, the biomass can be rapidly increased. Finally, the removal rate of pollutants in the dairy farm wastewater can reach more than 80%, and the biomass yield is increased by 2.3 times. In addition, Synechococcus ZSL-NS1 can also produce chlorophyll, protein, polysaccharide, oil and other biomasses, so that the purpose of recycling is achieved while the pollution is removed, and the method has application value.

The present application relates to a kind of degradation methods of polyethylene microplastics, comprising the following steps: 1) the MP is removed surface moisture;2) the MP of surface moisture removal is placed in deionized water, is treated with ultrasound, and obtain uniform MP suspension;3) the pH of MP suspension is adjusted to 5-8;4) in the suspension of adjusting PH value, PMS solid powder is added, and stirring is until PMS is completely dissolved, and obtain mixture;5) the mixture obtained in step 4) is degraded under the irradiation of VUV lamp reaction.Using the method of the present application, the degradation rate of MP can reach 51.5%, and the toxicity of degradation solution is limited, which can be used as the carbon source for the growth of chlorella.The present application utilizes the synergistic degradation effect of VUV photolysis and sulfate radical-based advanced oxidation system, realizes the efficient degradation of MP under mild conditions, and opens up a new way for designing practical MP pollution remediation technology.

The invention discloses a microalgae carbon sequestration system and method, and belongs to the field of carbon dioxide capture and utilization. The system comprises a natural pond, a gas supply unit, a photobioreactor, an MBR separation tank and the like, and stable operation is guaranteed through PLC automatic control and pipeline electric heat tracing. According to the method, wild type marine nannochloropsis oculata is taken as a material, CO2 gradient domestication is carried out, variable light intensity, bacteria-algae synergism and precise nutrition addition culture are combined, and then separation, concentration, dehydration and algal fat extraction are carried out to prepare the biodiesel. According to the method, the cost is reduced and the water body is purified by utilizing a natural pond water source, the microalgae carbon sequestration efficiency and biomass are remarkably improved, CO2 in a steel mill is converted into high-value biodiesel, and a low-cost and sustainable scheme is provided for carbon neutrality.

The utility model relates to the field of wastewater toxicity detection, and discloses wastewater toxicity detection equipment based on chlorella photosynthesis oxygen production rate, which comprises a magnetic stirrer (1) and a reactor arranged on the magnetic stirrer (1), the reactor comprises an outer shell (2) and an inner shell (3), a detection chamber (4) for detecting wastewater is formed in the inner shell (3), and the inner shell (3) is internally provided with a water inlet (5) and a water outlet (6). A gap between the outer shell (2) and the inner shell (3) forms a cooling chamber (5) for providing a constant-temperature environment for the detection chamber (4), a magnetic rotor (6) is arranged at the bottom of the detection chamber (4), and a dissolved oxygen probe (7) is arranged at the upper part of the detection chamber (4); a light source (11) is arranged outside the cooling chamber (5), and a light shielding device is arranged outside the light source (11), so that the influence of exogenous light on the photosynthesis of algae is eliminated, the oxygen production rate of the photosynthesis of chlorella is detected, and the toxicity of wastewater is rapidly and sensitively detected.

The present disclosure relates to a method of culturing a phototrophic organism in an aqueous medium under culture conditions comprising light and a gas atmosphere comprising carbon dioxide. The culture can be carried out continuously. The methods may be used to capture carbon dioxide, and / or to produce organic feedstocks that may be used in downstream processes, such as to produce biofuels and / or biofertilizers. The present disclosure also relates to strains of Chlorella species adapted to CO2.

The invention provides a method for culturing microalgae by using beer waste liquid and application of the method, and relates to the technical field of microalgae biological culture. According to the method disclosed by the invention, microalgae, especially chlorella sp. Of chlorophyta, are cultured by adopting the beer wastewater. Moreover, it is found for the first time that the effect of synergistically promoting the growth of the microalgae can be achieved when fermentation tank washing water is adopted as a nutritional ingredient to jointly culture chlorella (Chlorella sp.) FACHB-2900 and Navicula sp. FACHB-1996, and the composite microalgae can keep certain biological activity at a low temperature and also has excellent synergic cold resistance. The invention also provides a preparation method of the composite microalgae related fertilizer, and the microalgae fertilizer can be used for improving the soil performance of saline-alkali soil, and has excellent performances of promoting the growth of beet, increasing the yield and the like.

The invention discloses a one-step seedling forming culture medium for tissue culture of retina lepiderei and a seedling forming method, and belongs to the field of tissue culture of retina lepiderei. The invention aims to solve the problems that the conventional culture medium is easy to cause malformation, vitrification and other bad phenotypes of tissue culture seedlings, and also possibly causes genetic instability. The culture medium is composed of a 1 / 2 MS culture medium, wall-broken chlorella powder, wall-broken spirulina powder, cane sugar, agar and deionized water. According to the method, the browning phenomenon is effectively inhibited, and the photosynthetic efficiency is improved, so that the robust growth of tissue culture seedlings is accelerated, the tissue culture process of the retina lepidogramme is greatly simplified, and the use cost of hormones is remarkably reduced.

The invention involves mutant or recombinant Chlorophyte algal organisms that have a genetic modification in a gene encoding a chloroplastic signal recognition particle 43 (cpSRP43). In one embodiment the Chlorophyte organisms are Trebouxiophyte algae that are diploid or polyploid for a gene encoding a chloroplastic signal recognition particle 43 (cpSRP43). The mutant organisms can have a genetic modification in one allele of the gene but not in another allele of the gene. The mutant or algal organisms have higher biomass and lipid productivity. Additional mutant or algal organisms are disclosed that also have a genetic modification to one or more genes encoding a light harvesting chlorophyll a / b (binding) protein.

The invention relates to the field of environmental functional materials, and particularly provides a chlorella-magnetic nano composite adsorption material based on photosynthesis driven polymerization, a preparation method and application thereof. According to the invention, efficient capture of chlorella is realized by utilizing a magnetic nano material, and on the basis, dopamine in-situ polymerization is induced by virtue of photosynthesis of chlorella, so that surface functionalization of the composite adsorption material is realized, and the composite adsorption material has high adsorption performance, good stability and magnetic recoverability. In-situ coating of polydopamine is induced through photosynthesis, surface functionalization is achieved, the oxygen participation amount in dopamine in-situ polymerization is increased, the dopamine loading amount is further increased, and the environmental adaptability and the green synthesis characteristic of the material are further enhanced; and the triple challenges of sustainability, reproducibility and recoverability of the micro-plastic removal material are solved.

The application discloses a kind of fungal inoculant and its application in algae crust, belong to the field of microorganism.The technical problem solved by the application is how to construct algae crust.The application of fungal inoculant disclosed in the application in algae crust comprises the following steps: after dark endophytic fungi and microalgae are inoculated to the surface layer of soil to be repaired, culture is carried out, the construction of algae crust is realized;the dark endophytic fungi are the strain with the preservation number CGMCC No.17463 in China General Microbiological Culture Collection Center;the microalgae are composed of Microcoleus sp., Scenedesmus sp.and Chlorella sp.The application successfully realizes the rapid formation of algae crust, improves soil carbon accumulation, solves the key technical problems such as long crust period and poor adaptability, and is beneficial to the purpose of ecological restoration of degraded soil in arid and semiarid mining areas.

The invention discloses a preparation method for extracting algae doxine. The algae doxine is prepared from spirulina, chlorella, euglena, haematococcus, corn, maltose and yeast. The preparation method comprises the following steps: S1, raw material pretreatment, S2, steaming treatment, S3, cooling saccharification, S4, constant-temperature fermentation, S5, distillation treatment, S6, soaking process, S7, precipitation separation, and S8, filtering and sub-packaging. According to the preparation method for extracting the algae doxol, disclosed by the invention, a cell body in algae is obtained through the steps, and the product is green or dark green, shows blood red under light refraction and has the effects of resisting aging and supplementing nutrition for human cells.