529 results about "Chlorella" patented technology

The invention describes a pharmaceutical composition and method for treating cancer comprised of A) 2,3-dimethoxy-5-methyl-1,4-benzoquinone and/or B) at least one of wild yam root, teasel root, balm of gilead bud, bakuchi seed, dichroa root, kochia seed, kanta kari, bushy knotweed rhizome, arjun, babul chall bark, opopanax and bhumy amalaki; optionally one or more of frankincense, garcinia fruit, vitex, dragons blood, mace, sage and red sandalwood with at least c) one compound capable of maximizing oxidative mitochondrial function preferably riboflavin or vitamin B₂ derivatives, FAD, FMN, 5-amino-6-(5′-phosphoribitylamino)uracil, 6,7-Dimethyl-8-(1-D-ribityl)lumazine, ribitol, 5,6-dimethylbenzimidazole, tetrahydrobiopterin, vitamin B₁, lipoic acid, biotin, vitamin B₆, vitamin B₁₂, folate, niacin, vitamin C and pantothenate and/or d) at least one lactic acid dehydrogenase inhibitor (preferably 2′,3,4′5,7-pentahydroxyflavone) and optionally f) an alkalizing agent (aloe vera, chlorella, wheat grass, sodium or potassium bicarbonate, potassium) g) an antiproliferative herb (speranskia or goldenseal) and h) a pharmaceutically acceptable carrier.

The invention describes a nutraceutical composition and method for preventing/treating cancer comprised of A) quinones (2,3-dimethoxy-5-methyl-1,4-benzoquinone, thymoquinone, tocopherolquinone) B) compounds capable of maximizing oxidative mitochondrial function preferably riboflavin, FAD, FMN, 6,7-Dimethyl-8-(1-D-ribityl)lumazine, ribitol, 5,6-dimethylbenzimidazole, tetrahydrobiopterin, vitamin B₁, lipoic acid, biotin, vitamin B₆, vitamin B₁₂, folate, B3, C and pantothenate C) lactic acid dehydrogenase inhibitors; 2′,3,4′5,7-pentahydroxyflavone, epigallocatechin gallate, quercetin, citric acid, rosemary, black walnut, clove, nutmeg, licorice root, coriander, cinnamon, ginger root, myrrh gum and green tea D) alkalizing agents: aloe vera, chlorella, wheat grass, apple cider vinegar, burdock root, kudzu root, alfalfa, barley grass, spirulina, parsley leaf, calcium, magnesium, potassium or bicarbonate salts E) potent tumoricidal herbs; gromwell root, wild yam, beth root, teasel root, balm of gilead bud, frankincense, bakuchi seed, dichroa root, kochia seed, kanta kari, sweet myrrh, galbanum, garcinia fruit, mace, white sage and tumoricial plant derived constituents: gambogic acid, shikonin, diosmin or boswellic acid F) an antiproliferative herb (speranskia or goldenseal) and G) a pharmaceutically acceptable carrier.

The invention discloses a small ball algae cultivation method with high density and high quality, which comprises the following phases: high-density heterotrophy cultivation, high-density algae liquid dilution and photo-autotroph cultivation. The invention makes the small ball algae reach high-density level within shorter cultivation period, which improves the small ball algae quality.

The invention discloses a chlorella which belongs to the technical field of producing biodiesel and a cultivating method thereof. The chlorella is named as Chlorella YJ1, and the registration number of the spawn preservation is CGMCC No. 3037. The invention also discloses the application of the chlorella in producing biodiesel. Second-grade effluent water of municipal sewage is taken as the culture medium to grow the chlorella without adding nutrient elements such as nitrogen, phosphorus and the like, thus reducing the cost of producing biodiesel and lightening the burden of water resource. The chlorella is used for establishing the process of municipal sewage deep treatment, and the biodiesel can be obtained at the same time of the sewage treatment, which is a sewage recycling and biodiesel producing technology with eco-friendliness and low cost.

The invention provides a method for detecting microalgae oil, belonging to the field of oil detection. The method solves the technical problems that the needed sample is large and the consumed time is long in the existing weighing method. The method comprises the following steps: 1, diluting microalgae culture solution to obtain diluent; 2, detecting the fluorescence intensity of the dyed diluent; 3, centrifuging the diluent, and detecting the fluorescence intensity of the dyed supernate; 4, detecting the fluorescence intensity of the undyed diluent; and 5, computing the maximum difference of the fluorescence intensity, and computing the content of the microalgae oil according to a fluorescence intensity-oil content standard curve. The method is applied to detecting the unicellular algae such as the chlorella, the scenedesmus, the spirulina, the diatom, the dinoflagellate, the chrysophyceae, the euglenophyta, the stonewort and the like, and is applied to detecting the macro algae which is preprocessed by cell discruption.

The invention provides a method for inducing fresh water chlorella to fast accumulate grease through plant hormone jasmonic acid. The method is characterized by comprising the following steps of: (1) preparation of chlorella solution preparation: under the conditions that the temperature is 22-24 DEG C, the light intensity is 1800-2000lx, and the light-to-dark ratio is 12h/12h, culturing fresh water chlorella to logarithmic phase to obtain chlorella solution for conducting hormone induction, wherein the fresh water chlorella adopts NO.9 fresh water chlorella or NO.31 fresh water chlorella which both come from Freshwater Algae Culture Collection at the Institute of Hydrobiology, FACHB-collection; (2) acquisition of high-grease-content chlorella: adding jasmonic acid into 200ml of chlorella solution obtained in step (1) until the final concentration is 0.49-0.51mg/l; and (3) acquisition of high-grease-content chlorella: culturing the chlorella solution subjected to hormone induction in step (2) for 15 days under the conditions that the temperature is 22-24 DEG C, the light intensity is 1800-2000lx, and the light-to-dark ratio of 12h/12h, and centrifugally collecting the frond with high grease content. The method is simple and easy, low in cost, and the grease content of the target product is high, and can be used as a raw material of industrialized biological produced oil.

The invention belongs to the technical field of biology and relates to a method for producing a microecological foliar fertilizer by utilizing probiotics and microalgae. The method comprises the following steps of: firstly, culturing to obtain liquid culture prepared by bacillus, lactic acid bacteria and rhodopseudomonas palustris with the cell concentration being 10 billion/ml and saccharomycetes and chlorella with the cell concentration being 2 billion/ml respectively, then preparing liquid culture with four types of probiotics and one type of chlorella, and mixing the liquid cultures according to respective volume ratio to obtain the liquid microecological foliar fertilizer with the total cell concentration being more than 5 billion/ml. The method has the advantages that the used strains and chlorella are all probiotics which can be eaten by human, and are safe and non-toxic; the microecological foliar fertilizer integrates the advantages of promoting the plant growth of the probiotics, the chlorella and metabolites thereof; and the microecological foliar fertilizer is diluted with water by 100 times every 10 days and is sprayed on plant leaves, and the plant yield is increasedby more than 30% in 60 days. The method has an important application value in aspect of improving the yield and the quality of crops such as grain and the like.

The invention relates to a method for rapidly cultivating chlorella and belongs to the technical field of biology. The cultivation method comprises the following steps: preparing a standard BG-11 culture medium to serve as a basal culture medium of chlorella by taking fresh running water as a solvent; adding a certain amount of organic carbon source and NaHCO3 into the standard culture medium, so that the final concentration is respectively 0.5-1.0g/L and 0.2-0.5g/L; putting the culture medium into a culture vessel, inoculating 10-20 percent of chlorella solution, sealing the opening, and culturing the chlorella solution in an environment with the illumination intensity of 3000-4000Lx, the light-dark ratio of 12h:12h and the temperature of 22-28 DEG C. According to the method, the cell density of the chlorella can reach a high level in a short cultivating cycle and is increased by about six times compared with that cultivated by using the basal culture medium. Meanwhile, the method has the advantages that the process is easy to operate, the culture medium is not required to be sterilized, the pH value of the culture medium is not required to be regulated, and the method can be widely applied to cultivating chlorella in industrial production.

The invention discloses a chlorella cell wall disruption method which includes the following steps: step a: washing chlorella concentrate culture fluid through clear water to remove the culture solution therein so as to get chlorella concentrate liquid; step b: freezing the chlorella concentrate liquid quickly, then heating to enable the frozen chlorella concentrate liquid to thaw quickly; and step c: mechanically grinding the chlorella concentrate liquid obtained from the step b. The chlorella cell wall disruption method is simple in operation, low in cost and low in requirement for the equipment, and the cell wall of the chlorella can be disrupted at high efficiency. Meanwhile, high temperature and high pressure are not needed and no chemicals are necessary to be added, so the nutrition of the chlorella can be preserved to the maximum and green, safe and edible chlorella with the cell wall disrupted can be obtained.

The invention relates to a culture medium composition suitable for culturing ordinary chlorella and a method for mixed culture of the chlorella by using the culture medium. The culture medium is obtained by adding 0.00126-0.063 g/L of Na2SO3 and an organic carbon source based on a common SE culture medium. The use of the culture medium in photoautotrophy-heterotrophy mixed culture of the ordinarychlorella can remarkably promote the growth of chlorella, and can allow the cell density of the ordinary chlorella to reach a level of high density culture in a short culture period, that is, the high density culture of the chlorella is realized. The use of the culture medium composition allows the ordinary chlorella to easily use the organic carbon source, especially a cheap carbon source for growing, can promote the later grease accumulation, and can greatly increase the quality of the ordinary chlorella, i.e. the grease content of the ordinary chlorella compared with heterotrophic culture to reach the level of photoautotrophic culture.

The invention relates to lipid-rich chlorella and culture application thereof. The strain is SF-B1, is classified and named as Chlorella sp., and has been preserved in the General Microbiological Center of the China Microbiological Culture Collection Management Committee on July 6, 2015, and the preservation number is CGMCC No. 11005. The chlorella can tolerate high-concentration CO2 and NOx, thecarbon fixation efficiency is high, and the total lipid content of cells in obtained biomass is 45% or above of the dry weight of the cells.

The invention discloses a method for removing nitrogen and phosphorus in breeding wastewater through immobilized algal-bacteria, and belongs to the field of water purification. The method is characterized in that a chlorella and activated sludge symbiotic system is utilized for absorbing and utilizing nitrogen and phosphorus in breeding wastewater, and sodium alginate is capable of immobilizing, and on that basis, the wastewater can be cleaned through chlorella and activated sludge, and meanwhile, algae and water can be separated. The method has the advantages that the breeding wastewater is treated through sodium alginate by immobilizing chlorella and activated sludge, and the gradually-increased pressure of fresh water is relieved; in addition, chlorella and activated sludge are capableof absorbing and utilizing nitrogen and phosphorus in breeding wastewater, so that the wastewater can be purified, and moreover, the possibility that the wastewater is drained into natural water to cause eutrophication can be reduced; in addition, the immobilized chlorella and activated sludge are capable of separating algae and water during treating the wastewater, so that the convenience of continuous massive wastewater treatment is ensured.

The invention discloses a probiotics fermented chlorella beverage and a production method thereof, belonging to the technical field of health beverages. The beverage is prepared from the following materials in percentage by weight: 15%-25% of probiotics, 4%-8% of chlorella, 1%-3% of fructooligosaccharide, 6%-16% of a sweetening agent and the balance of water. The probiotics fermented chlorella beverage is prepared by the following steps: carrying out wall breaking on a manually-prepared chlorella concentrated solution; adding water for deactivating and removing odor and then adding fructooligosaccharide and the sweetening agent; removing dreg and disinfecting; then inoculating the probiotics in a fermentation tank and fermenting at 30-35 DEG C for 36-48 hours; then fermenting at a room temperature of 25 DEG C for 24-48 hours to prepare fermented liquid; and centrifuging to obtain the probiotics fermented chlorella beverage. According to the probiotics fermented chlorella beverage, the chlorella with broken walls is adopted so that CGF (chlorella growth factor) functional substances in the chlorella can be used for improving the regeneration capacity of normal cells to be more than 25%; the quality and the character of the probiotics fermented chlorella beverage are good; and the raw materials is readily available, a processing method is simple and easy, the economic benefits are high, and thus the probiotics fermented chlorella beverage has wide market development prospects.

The invention belongs to the field of renewable energy, and discloses a method for extracting heterotrophic chlorella neutral oil. According to the invention, a large amount of heterotrophic chlorella cultivated by fermentation in a bioreactor is adopted as a raw material. With mechanism crushing of a ball mill, with an auxiliary mass transfer effect of ultrasonic waves, and with the soakage of a low-pole organic solvent, cell walls of dried chlorella and cell walls of chlorella that is not dried or dehydrated are crushed; the material is extracted by using a neutral solvent, such that crude chlorella neutral oil is obtained. For completely dried chlorella cells, the extraction rate of neutral oil reaches 99.27%. For water-containing chlorella cells, the extraction rate of neutral oil reaches 89.61%. With the method, the obtained neutral oil can be directly used for preparing biodiesel, and the chlorella neutral oil extraction time is shortened. Therefore, the method has industrial production potential. With the method, a large amount of raw material oil is provided for large-scale productions of biodiesel. The method is a rapid and simple chlorella neutral oil extraction approach.

The invention discloses a chlorella drink and a manufacturing method thereof. The chlorella drink comprises cell wall-broken chlorella and water, wherein the cell wall-broken chlorella accounts for 1 to 3 percent of the total weight. The method for manufacturing the chlorella drink comprises the following steps: adding cell wall-broken chlorella concentrated solution into water and uniformly stirring through a material preparing step; and through a canning step, clarifying the mixture, removing dregs from the mixture, canning the mixture and sterilizing the canned chlorella drink to obtain the chlorella drink. The chlorella drink has the advantages of low cost, convenient drinking, safety, environmental friendliness and no pollution. The cell wall-broken chlorella contains a plurality of nutritive materials including CGF. The chlorella drink is easily absorbed by human bodies, has a good health-care function and can be drunk by people in daily life.

The invention discloses a Chlorella autotrophic-heterotrophic mixed culture method, belonging to the technical field of biology. The method comprises the following steps: heterotrophic culture of Chlorella in mechanical-stirring fermentation tank, Chlorella solution dilution, and optical autotrophic culture of Chlorella. By using the method, the Chlorella can achieve high-density culture level in a short culture period, and the quality of Chlorella is greatly enhanced as compared with that of the Chlorella subjected to heterotrophic culture, thereby achieving the optical autotrophic culture level and implementing high-density high-quality culture of Chlorella.

The invention discloses a method for treating aquaculture wastewater through immobilized chlorella and belongs to the field of water purification. By utilizing absorption and utilization of chlorellaon nutritive materials and other trace elements in the wastewater and immobilization on sodium alga acid, alga-water separation can be well performed when the chlorella achieves an effect of purifyingthe wastewater. The method has the advantages that the aquaculture wastewater is treated by chlorella immobilized by the sodium alga acid, and increasingly severe fresh water pressure is relieved; moreover, due to absorption and utilization of the chlorella on various elements in the wastewater, the wastewater is purified, and the load of the wastewater discharged into natural water finally is further retarded; in addition, after the chlorella is immobilized, the alga-water separation can be well realized, and equipment cost is reduced.

The invention discloses applications of algae in a solid beverage and formulae. The solid beverage contains broken-wall deactivated chlorella or spirulina, a flavoring agent, a food additive, an auxiliary material and the like. The algae solid beverage is characterized in that the flavoring agent, the food additive, the auxiliary material and the like are added in a compounding step, and then the process steps of mixing, drying, crushing, sieving, packaging, disinfection and the like are carried out to obtain the finished product. By adopting the technical scheme, a variety of different types of products and products with different flavors can be produced by adjusting the product formulae. The product has the characteristics of having comprehensive nutrition, and being mellow and delicious in taste, convenient to carry, easy to store, convenient to use, safe and healthy.

The invention provides a method for purifying piggery fermentation wastewater by using Chlorella, which comprises the following steps: (1) pretreating piggery fermentation wastewater, and adjusting pH to 6.5-7.5 and wastewater concentration in an adjusting tank; (2) acclimating and culturing Chlorella in wastewater to ultimately obtain a Chlorella strain which can rapidly grow in 50-70% (v/v) wastewater; (3) inoculating the acclimated Chlorella strain into wastewater, and culturing in a closed type photobioreactor; and (4) collecting Chlorella, and thus finishing wastewater treatment. The method of the invention uses Chlorella with high adaptability to purify piggery fermentation wastewater, can remove TN, TP and COD in wastewater, and solves the problem that in the prior art, the piggery fermentation wastewater is treated in an uneconomic traditional method or directly discharged to cause environmental pollution. In addition, the collected Chlorella can be used for other purposes to generate certain economic benefits.

The invention discloses a fruit and vegetable enzyme capable of protecting the liver and expelling toxins and a preparing method of the fruit and vegetable enzyme. The enzyme is prepared from 400-600 parts of pineapples, 50-150 parts of pawpaws, 50-150 parts of grapes, 50-150 parts of mulberries, 50-150 parts of blueberries, 50-150 parts of red dates, 50-150 parts of lemons, 500-700 parts of red beet roots, 50-150 parts of wheat grass, 50-150 parts of chlorella, 400-600 parts of saccharose and 10-20 parts of probiotics. The fruit and vegetable enzyme is not added with a preservative or artificial colors or medicine in the whole process, is not blended with fruit juice and is prepared through symbiotic fermentation by implanting various probiotics into various natural organic fruits, vegetables and other food materials as raw materials in a special health-maintenance formula, and the fruit and vegetable enzyme contains rich active enzymes and contains various vitamin complexes, mineral substances and microelements beneficial to the human body. Absorption and metabolism of the body can be regulated, and the purposes of toxin expelling and detoxifying of the liver and the absorption and metabolism of the human body can be effectively achieved. The fruit and vegetable enzyme is effective on migraine, the fatty liver, the alcoholic liver, cirrhosis, insomnia, astriction and diarrhea, has the effect of dispelling the effect of alcohol at the same time, and can be taken by pregnant women and children safely.

The invention aims to provide a biological preparation method of red nano selenium, which comprises the following steps: by using Chlorella as a biological material and a seleniferous inorganic salt as a selenium source, inoculating the Chlorella into the seleniferous salt culture medium, culturing under lighting conditions, and controlling the culture temperature at 22-28 DEG C to prepare the red nano selenium. In order to overcome the defects in the prior art, the Chlorella used as the culture material is cultured in the seleniferous salt BG-11 culture medium under certain light and temperature conditions to prepare the red nano selenium, thereby lowering the production cost of the nano selenium and developing new medicinal value and health care functions for pyrenoid Chlorella. The method is environment-friendly in the production process, and can not cause environmental pollution.