45results about How to "High live bacteria" patented technology

The invention relates to lactobacillus reuteri and application thereof. Lactobacillus reuteri is lactobacillus reuteri LZ262 which is preserved in the Common Microorganism Center of the China Committee for Culture Collection of Microorganisms on 13 August, 2015 with the preservation number of CGMCCNo.11240. Lactobacillus reuteri LZ262 is from the intestinal tract of a healthy pig, can be colonized in a human body and an animal body, and has higher tolerance to gastric acid and bile salt; in addition, lactobacillus reuteri LZ262 has a better suppression effect on Escherichia coli, and has a higher survival rate after spray-drying, and higher fermentation density is achieved by improving a fermentation formula; the strain has a better effect of synergistic growth with other types of lactic acid bacteria, can be used for food and animal feed additives, and can also be widely applied to other food and biological products.

The invention discloses an astragalus membranaceus micro-ecologic preparation as well as a preparation method and an application thereof, and belongs to the technical field of feed additives and biological fermentation tradition Chinese medicines. The micro-ecologic preparation is prepared by fermenting astragalus membranaceus, a lactic acid bacteria seed solution and a solid culture medium, wherein the lactic acid bacteria seed solution is prepared by mixing and culturing enterococcus faecium and lactobacillus plantarum at a ratio of (2-8): (8-2); the number of viable organisms in the inoculated lactic acid bacteria seed solution, corresponding to every 25-75 g of astragalus membranaceus, is 25*109; compared with the traditional additive which directly mixes the lactic acid bacteria and the astragalus membranaceus, the astragalus membranaceus micro-ecologic preparation has the effect that the yield of the crude polysaccharide and the quantity of the lactic acid bacteria are improved after fermentation. According to the preparation method of the astragalus membranaceus micro-ecologic preparation, the synergistic effect of the lactic acid bacteria and the astragalus membranaceus is improved by selecting proper fermentation temperature and fermentation time. The astragalus membranaceus micro-ecologic preparation is used as a livestock feed additive.

The invention discloses a culture medium for effectively prolonging the survival time of lactic acid bacteria and a preparation method of the culture medium. The culture medium for effectively prolonging the survival time of the lactic acid bacteria is prepared by taking peptone, yeast extract powder, mannan oligosaccharide, skim milk powder, defatted soybean meal, magnesium sulfate heptahydrate, manganese sulfate heptahydrate, dried corn steep liquor powder, a compound additive, sodium dihydrogen phosphate, sodium citrate, citric acid, sodium acetate, Tween-80 and saccharomyces cerevisiae as raw materials; in addition, the compound additive is a mixture of sodium carboxymethylcellulose, sodium alginate, bone meal, royal jelly acid, guar gum and xanthan gum. According to the culture medium for effectively prolonging the survival time of the lactic acid bacteria, the functions of all the raw materials are mutually cooperative, and higher variable bacteria quantity of the lactic acid bacteria can be kept at normal temperature for a long time; in addition, the culture medium has the advantages of simplicity and convenience in operation, easily-obtained raw materials, low cost and high homogenization degree of a product; the lactic acid bacteria is enabled to be more stable in property and higher in biological activity and can be stored for a longer time.

The invention discloses efficient bio-organic fertilizer which comprises straw, livestock excrement, leftovers, winery sewage, lees, bagasse, discarded traditional Chinese medicine, mildewed grain, magnesium nitrate, egg shells, waste paper, wheat bran, rape seed cake, bean pulp and strains. The efficient bio-organic fertilizer is free from toxicity, harmless, environmentally friendly and antibacterial, prevents diseases, and can improve and repair soil, increase organic matter of soil, improve the water and fertilizer preserving ability of the soil, increase the yield, prevent water from being polluted and protect environment; waste is used as a raw material, the problem of environmental pollution caused by industry, agriculture and life garbage is reduced, the waste is turned into wealth, nutritional ingredients of the organic fertilizer can be increased, crops grow well, and the yield and the nutritional ingredients of the crops are increased; multiple compound bacteria are fermented to form the fertilizer, the content of effective living bacteria is high, fermentation time is shortened obviously, working efficiency is improved, the multiple bacterial strains grow coordinately, and the shelf life can be prolonged to be 5 years.

The invention discloses a whey protein solid beverage containing N-acetylneuraminic acid. The beverage is prepared from sugar, concentrated whey protein powder, whole milk powder, N-acetylneuraminic acid, probiotics, prebiotics, ethylenediaminetetraacetic acid ferric sodium salt and casein phosphopeptides. According to the whey protein solid beverage, all raw materials are effectively combined, the immunity of all kinds of people including infants and young children can be effectively enhanced, the whey protein content is high, nutrition is rich, absorption is facilitated, and more sufficientnutrition and energy can be provided for drinkers.

The invention relates to the technical field of pharmaceuticals and discloses a female probiotic composition and a preparation method and application thereof and a female care product. By the female probiotic composition, technical problems of low strain effective survival rate and poor colonization performance in the prior art can be solved, uncomfortable symptoms of female private parts can be effectively improved, vaginal pH value regulation is realized, and vaginal flora balance is recovered. In addition, the female probiotic composition has the advantage of effectiveness in improvement ofgynecological inflammations such as vaginitis, pelvic inflammation and cervicitis.

The invention relates to a microorganism water purification agent and a preparation method thereof. The preparation method comprises following specific steps of: inoculating the agent onto a cane molasses medium, and fermenting for 24-120 hours at 25-35DEG C so as to cultivate the agent. The method for purifying the water quality of aquaculture water by the prepared microorganism water purification agent comprises the step of adding 15-18ml of the agent prepared by the method into one cube of aquaculture water. According to the method, the cane molasses medium is used for cultivating the agent, so that the amount of viable bacteria can be increased to the greatest extent, the amount of viable bacteria of bacillus subtilis, which plays a main role in purifying the water body, can be increased, and the purification effect of the aquaculture water is better; for the fermentation time, the amount of viable bacteria of the agent can be improved, furthermore, and the agent holds certain amount of sugar so as to provide nutrient for the bacteria, thus being beneficial to guaranteeing the quality; the agent prepared by the method can effectively solve the problem of damage and eutrophication of the quality of the aquaculture water, thus improving the quantity and quality of aquatic products.

The invention provides a straw micro-storage additive. The straw micro-storage additive is characterized by being prepared by fermenting lactic acid bacteria, brewers yeast, bacillus subtilis, actinomycetes, nitrogen-fixing bacteria, and a mould strain in a ratio of (75,300-10,000):(73,000-10,000):(600-500):(70-50):(15-10):1. A method for preparing the straw micro-storage additive comprises the following steps of: activating the strain, preparing seed liquid, sterilizing fermentation equipment and a culture medium, inoculating, designing fermentation parameters, collecting thallus, performingvacuum freeze drying, preparing a fermentation agent and the like. The straw micro-storage additive has the advantages of scientific proportioning, complete function, and high straw treatment performance. The scientific compatibility and mutual coordination of cellulose decomposing bacteria and beneficial bacteria in animal intestines can effectively improve nutrition structures of straws. After the straws in which the additive is added are fermented at the temperature of 37 DEG C for 15 days, the pH value can be reduced to 3.8, the content of crude protein is improved by about 8 percent, 15.05 percent of cellulose can be degraded, the water content is about 50 percent, the corruption rate is low, the color is yellowish, the texture is loose and soft, and the odor is mellow and strong; moreover, the preparation process is simple and practical.

The invention discloses a probiotic powdery agent. The probiotic powdery agent includes the following components in parts by mass: 5-10 parts of probiotic powder, 300-350 parts of a probiotic protection agent, and 20-30 parts of a sweetener, wherein the probiotic powder includes lactobacillus plantarum, bifidobacterium bifidum and lactobacillus casei, the total bacterial content of the probiotic powder is greater than 16 billion cfu/g, and the lactobacillus casei accounts for 55%-65% of a total weight of the probiotic powder; and the probiotic protection agent includes skimmed milk, inulin, trehalose, maltodextrin and cereal fiber powder, the skimmed milk accounts for 50%-60% of a total weight of the probiotic protection agent, and the cereal fiber powder accounts for 20%-30% of the totalweight of the probiotic protection agent. The probiotic powdery agent can effectively regulate intestinal flora, promote growth of probiotics in an intestine, and ensure the live bacteria amount of the probiotics in the intestine; the probiotic protection agent protects the probiotic powder, especially the cereal fibers cooperate with the skimmed milk, so that the loss of the probiotics in extremeenvironments such as stomach acid and bile can be reduced, and the live bacteria amount of the probiotics when the probiotics reach the intestine can be improved; and at the same time, the cereal fibers cooperate with the probiotics to realize intestinal conditioning.

The invention discloses a biological fermentation product for preventing and treating colitis and a preparation method thereof. The biological fermentation product has a certain preventing and treating effect to the colitis, and is high in stability. Compared with a control group, the viable bacteria quantity and polysaccharide content of fermented clostridium butyricum are greatly increased, thefact that dimocarpus longan extract liquid can promote the growth of the clostridium butyricum is demonstrated, the performance of a product containing a clostridium butyricum probiotics preparation is further improved, and the biological fermentation product plays an important role in the bioengineering fermentation field.

The invention relates a Lactobacillus acidophilus and an application thereof. The Lactobacillus acidophilus is Lactobacillus acidophilus FZU-LA1301X with the preservation number of CGMCC No.12453, and is preserved in China General Microbiological Culture Collection Center on May 16, 2016. The Lactobacillus acidophilus FZU-LA1301X can be colonized in weaned piglets, has strong tolerance to gastric acid, bile salt and intestinal juice, has a good live bacterium number in an improved fermentation medium, has a high survival rate after being spray-dried, and can be used in animal feed additives.

The invention discloses a preparation method of probiotic gel particles. The probiotic gel particles are prepared from beta-cyclodextrine, sodium alginate, konjac glucomannan, edible oil and fat, a probiotic culture solution, a probiotic protecting agent and water through the steps of sol solution preparation, probiotic culture solution preparation, probiotic powder coagulant preparation, probiotic mixed base material preparation, probiotic sol preparation and probiotic gel particle preparation. The probiotic gel particles can be added into various probiotic liquid beverages. The probiotic gelparticles prepared by the invention can keep the activity of probiotics in probiotic liquid beverages, and are particularly applicable to beverages with demand for long-term quality retention. Through specific probiotic culture and probiotic powder coagulant preparation, probiotic loss in extreme environments such as gastric acid and bile is effectively reduced, and the amount of viable bacteriareaching the intestinal tract is increased.

The invention discloses a preparing method and application of high-density cultured nitrogen-fixing bacteria, and belongs to the technical field of bioengineering. The culture method comprises the steps that biochar serves as an adsorbent, nitrogen-fixing bacteria serve as an original strain, test tube expansion culture, liquid shake flask culture, seed tank expansion culture and liquid fermentation culture are carried out in sequence, and the average thallus density of the cultured nitrogen-fixing bacteria reaches 5*1013-1*1015 cfu/mL. Obtained fermentation liquid can be subjected to centrifugation and spray drying, and the quantity of viable bacteria in dried thallus powder reaches up to 1015-1017 cfu/g. The obtained nitrogen-fixing bacteria can serve as a live bacteria agent of bio-fertilizer or as a live bacteria agent of bio-fertilizer together with other strains.