The invention discloses a formula of earthworm protein health care wine. The formula comprises the following raw materials in parts by weight: 21-25 parts of earthworm protein containing 65% of protein, 6-10 parts of rhizoma polygonati, 6-10 parts of fructus jujubae, 11-15 parts of wolfberry fruit, 11-15 parts of dried longan pulp, 6-10 parts of fructus crataegi, 6-10 parts of fructus rubi, 6-10 parts of honey and 3.3 parts of liquor. The invention also provides a production method of the earthworm protein health care wine. The production method comprises the following steps: weighing, cleaning, dicing, roasting and boiling, drying, grinding, sieving and cellaring. According to the formula and the production method provided by the invention, by adopting the earthworm protein, the rhizoma polygonati, the fructus jujubae, the wolfberry fruit, the dried longan pulp, the fructus crataegi, the fructus rubi and the honey as raw materials for making wine, the functions of reducing and relieving hypertension, strengthening the body and improving absorption of nutrients by stomach are realized; moreover, with a simple wine making process, the formula is suitable for home making as well as for large-scale production in winery.