14 results about "Fusel alcohol" patented technology

The invention discloses saccharomyces cerevisiae XJ-YSSCE010-1 with high temperature resistance, high ethanol yield and low fusel oil yield and application of the saccharomyces cerevisiae XJ-YSSCE010-1, and belongs to the technical field of microbial fermentation. The saccharomyces cerevisiae XJ-YSSCE010-1 disclosed by the invention is separated and screened from Maotai-flavor liquor yeast, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.31174. The fusel oil comprises the following main components: n-propanol, isobutanol, isoamyl alcohol, phenethyl alcohol and the like, wherein isobutanol and isoamyl alcohol have the highest toxicity. The saccharomyces cerevisiae XJ-YSSCE010-1 disclosed by the invention shows the advantages of high yield of ethanol, low yield of fusel oil and no production of isobutanol and isoamyl alcohol in liquid fermentation and simulated high-temperature stacking fermentation experiments, and the saccharomyces cerevisiae XJ-YSSCE010-1 is applied to preparation of white spirit, so that the production efficiency of the white spirit is improved, and the quality of the white spirit is improved.

The application discloses a brewing method for improving the quality of a light-flavor type base liquor by using medicinal and edible Chinese herbal medicines, four kinds of medicinal and edible Chinese herbal medicines, namely, clove, mulberry leaf, lophatherum gracile and osmanthus, are adopted, and the suitable adding link and adding proportion of the medicinal and edible Chinese herbal medicines in the brewing process are determined. Compared with the traditional brewing process, the content of fusel oil (defect component) in the base liquor produced by the application is reduced by 13.89%, the content of ethyl acetate (important flavor component) is increased by 71.14%, the content of beta-damascenone is increased by 240.36%, and the contents of defect components such as acetaldehyde, methanol, n-propanol and fusel oil are all significantly reduced.

This invention discloses a sake brewing process using highland barley as raw material and its application, belonging to the field of fermented wine brewing technology. The process includes: after finely peeling the raw material, controlling the temperature of the added grain-moistening water to include, but not limited to, 15℃~25℃, and moistening the grain with water at a mass ratio of 115%~125% for 16~18 hours; steaming the grain under normal pressure for 13~17 minutes after steaming to promote deep starch gelatinization; spreading and cooling the grain mash until the core temperature is 17℃~19℃, then mixing it with baijiu koji at a low temperature; subsequently implementing a two-stage slurry control mode of "first solid-state stacking saccharification, then adding 75%~85% external water for dilution fermentation"; after fermentation, the mash is coarsely filtered and then precisely filtered through a 0.22~0.45um microporous membrane, combined with instantaneous heat passivation at 68℃~72℃ and rapid cooling. This invention not only increases the yield of finished wine by 8.7% to 11.3%, but also reduces the content of fusel oil by more than 60%, overcoming the industry pain points of non-distilled barley fermented wine being prone to loss of luster and sedimentation during its shelf life. The finished wine is clear and pure, with a typical and elegant aroma of the original grain.

The invention relates to the technical field of wine brewing engineering, and discloses a subsection temperature control fermentation-based bubble rice wine flavor upgrading process which comprises the following steps: firstly, gelatinizing glutinous rice, adding a component containing ultramicro citrus fiber and pectinase for enzymolysis modification, and constructing a bubble solid-liquid interface; adding a stress resistance regulator and compound yeast, and carrying out proliferation fermentation at 22-24 DEG C; then cooling to 10-12 DEG C, and standing to accumulate an ester precursor; raising the temperature to carry out dissolved oxygen pulse and nitrogen stripping; and finally, sealing and heating to 16-18 DEG C, and completing bubble stabilization by utilizing metabolism gas production. According to the method, the Pickering interface is constructed by utilizing the enzymolysis fiber, so that the bubble stability is effectively improved; by combining segmented temperature control with zinc ion regulation and control of metabolic flux, fusel oil generation is reduced, and ester accumulation is improved; yeast autolysis is prevented and volatile peculiar smell is removed by utilizing thermal oxygen pulse and nitrogen stripping, so that the bubble rice wine with fine and lasting bubbles, prominent ester fragrance and clean and refreshing taste is obtained.

The invention discloses a method for separating and refining fusel oil in Fischer-Tropsch synthesis, and belongs to the technical field of chemical separation. In order to solve the problems of difficult separation and high energy consumption of fusel oil due to the existence of azeotrope, the invention provides a rectification and membrane separation coupled process. The method comprises the following steps: firstly, pretreating a fusel oil raw material through a two-stage rectifying tower, and sequentially removing water, heavy components and light components; then carrying out neutralization desalination treatment on the obtained mixed alcohol solution, and then feeding the mixed alcohol solution into a steam permeable membrane system for deep dehydration to obtain mixed alcohol steam with low water content; and finally, sequentially feeding the dehydrated alcohol steam into a subsequent rectifying tower, and separating to obtain high-purity ethanol, n-propyl alcohol and n-butyl alcohol products. Through energy integration design, high-temperature material flow is used for preheating raw materials and providing a heat source for the rectifying tower, and energy consumption is remarkably reduced. The method is reasonable in process, effectively breaks through the alcohol-water azeotropic limitation, and realizes efficient and low-cost separation of high-added-value components in fusel oil.

The invention discloses a brewing process of fen-flavor liquor more suitable for being used as base liquor of health-care liquor, which is characterized in that through three improvements of a grain crushing, grain moistening and cooking process, functional bacterial liquid adding in a pool and short-time fermentation, flavor components such as acid ester in the fen-flavor liquor are further reduced, and defective components are further reduced; meanwhile, the wine rate is not influenced, and the wine body is clearer than the wine body prepared by the original process. The method can effectively reduce the content of total acids and total esters and defective components such as fusel oil in the raw wine, and has important significance for improving the quality of the base wine of the health-care wine.

The invention provides a green plum wine brewing method and equipment. The green plum wine brewing method comprises the following steps: S1, green plum pretreatment: manually picking and removing unqualified green plums and impurities; cleaning the green plum raw material by a bubble cleaning machine; and the cleaned green plums are air-dried by an air dryer. The qualified green plums are conveyed into a firing pin type green plum pitting machine to remove plum pits, and green plum fruits and fruit pits are automatically output in a split-flow mode. S3, juicing: juicing the pitted green plums by using a pneumatic spiral juicer, and inputting green plum juice into a refrigerant temperature control fermentation tank by using a sealed fruit juice delivery pump; s4, performing three-stage gradient fermentation: performing enzymolysis at the first stage, and controlling the temperature to be 16-20 DEG C; in the second stage, fermentation is started, and the temperature of the fermentation tank is controlled to be 18-25 DEG C; and in the third stage, the temperature of the fermentation tank is controlled at 25-30 DEG C. Through denucleating, juicing and fermentation, the wine body is free of bitter taste, the clarity, the perception degree and the health degree of the green plum wine are effectively improved, three-stage temperature control is adopted for fermentation, the production of fusel oil and methanol is effectively controlled, and the quality of the wine body is improved.

The present application relates to the field of genetic engineering and enzyme engineering, in particular to a lipase mutant capable of esterifying short-chain fatty acids in aqueous phase, a construction method and application thereof. Based on a Burkholderia-derived lipase, two single-point mutants H24T and A191M and a double-point combination mutant H24T-A191M are screened. The mutant can catalyze the esterification reaction of isoamyl alcohol and acetic acid under high water activity conditions, and the esterification product is less affected by hydrolysis. The mutant obtained by the present application is more suitable for industrial production than the natural lipase, and can be particularly used in the field of removing fusel oil and catalyzing aging of liquor, and has good application prospect and industrial value.

The invention discloses a lipase mutant and application thereof, and belongs to the field of gene engineering and enzyme engineering. On the basis of staphylococcus epidermidis-derived lipase, two single-point mutants A86K and Q125L and a two-point combined mutant A86K-Q125L are obtained through screening, the reaction specificity to isoamyl alcohol is improved by the mutants, the reaction specificity to ethanol is reduced by the mutants, and the three mutants can catalyze esterification reaction under the condition of high water activity. The mutants provided by the invention are more suitable for industrial production than natural lipase, especially in the field of fusel oil removal of Baijiu, and have very huge application prospects and industrial values.

The utility model discloses a separating and refining device for fusel oil in Fischer-Tropsch synthesis, which comprises a pressurized rectifying tower used for carrying out pressurized rectifying treatment on the fusel oil to remove acids, C4 + alcohol and part of n-butyl alcohol; the membrane separation unit is connected with a top discharge hole of the pressurized rectifying tower and is used for carrying out dehydration treatment on tower top steam; the normal-pressure rectifying tower I is connected with a bottom discharge hole of the membrane separation unit and is used for removing light components; the reduced-pressure rectifying tower is connected with a bottom discharge hole of the normal-pressure rectifying tower I and is used for removing propyl alcohol and butanol in tower bottoms; the normal-pressure rectifying tower II is connected with a bottom discharge hole of the reduced-pressure rectifying tower and is used for removing isopropanol in tower bottoms; and the normal-pressure rectifying tower III is connected with a bottom discharge hole of the normal-pressure rectifying tower II and is used for separating n-propyl alcohol and n-butyl alcohol in tower bottoms. According to the device disclosed by the utility model, step-by-step efficient separation and resource utilization of components with different polarities and boiling points in fusel oil are realized through cooperative coupling of multi-stage rectification and membrane separation.

The invention relates to the technical field of chemical engineering, in particular to a rectification device for improving fusel oil purity, which comprises a fusel oil raw material preheater, a fusel oil rectification tower and a rectifier, an outlet of the raw material preheater is connected with a raw material inlet of the fusel oil rectifying tower; 95 alcohol-based fuel is distilled from the top of the fusel oil rectifying tower, a first reflux inlet is formed in the side wall of the upper part of the fusel oil rectifying tower, and a first recovery assembly for recovering and purifying 95 alcohol is arranged at the first reflux inlet; a tower plate is arranged at the lower part in the fusel oil rectifying tower; a gas phase side extraction opening is formed in the middle of the fusel oil rectifying tower; according to the invention, the water content in the extracted material can be reduced by designing and newly adding the gas phase side extraction port and the rectifier, the rectifier is provided with the condenser and the reflux tank, and the heat of the side extraction gas phase is utilized to realize partial separation of organic matters and water, so that 70 alcohol is distilled from the top of the rectifier, and the production efficiency is improved. Wastewater containing organic matters at the bottom automatically flows into the fusel oil rectifying tower to be continuously separated.

The invention relates to the field of gene engineering and enzyme engineering, in particular to a lipase mutant capable of esterifying short-chain fatty acid in a water phase as well as a construction method and application thereof. According to the invention, two single-point mutations H24T and A191M and a two-point combined mutant H24T-A191M are obtained by screening on the basis of the burkholderia source lipase. The mutant can catalyze the esterification reaction of isoamyl alcohol and acetic acid under the condition of high water activity, and the esterification product is less affected by hydrolysis. The mutant obtained by the invention is more suitable for industrial production than natural lipase, especially can be used in the fields of fusel oil removal and aging acceleration of Baijiu, and has good application prospects and industrial values.

The invention relates to the technical field of white spirit brewing, and discloses a preparation technology of a flower-fragrance wine pool containing half-dry flower pollen and a flower-fragrance wine brewing method.The preparation technology comprises the steps that mud, half-dry flowers, pollen, honey and rice husks in the ancient runner of the Yellow River are selected, and no exogenous water is added in the whole stirring and filling process; and after natural curing, a functional pool wall which is free from peeling and slag falling is formed. The brewing method comprises the following steps: soaking raw materials by adopting multi-temperature-section heating, and extracting half-dried petals by utilizing high-temperature waste heat; after distiller's yeast is added, the mixture is put into the floral wine pool for sealed fermentation; scattering and airing after fermentation is finished, performing circulating distillation for 3-5 times by virtue of small molecule distillation equipment, accurately cutting out wine cores of 70-85 degrees, and finally sealing and ripening the wine cores in a wooden barrel. According to the method, the problem that a traditional mud pool is prone to cracking is effectively solved, and the high-quality white spirit which is low in fusel oil content, rich and elegant in flower fragrance and clean, cool and soft in taste is obtained through the unique solid state fermentation interface and the circulation distillation technology.