The invention discloses a production method of grape seed oil, which belongs to the technical field of the food processing. Grape seeds are cleaned and broken, the moisture content of the grape seeds is adjusted, and the refined grape seed oil is produced through the procedures such as pre-heat treatment, enzymolysis, microwave treatment, post heat treatment, drying, squeezing, alkali refining, hydration, heat-cold treatment, decoloration and filtering. With the production method, the oil yield reaches 95 percent or more, procyanidin extraction rate can reach 60 percent or more, the destroy degree of other ingredients such as oilmeal protein after the oil pressing is small, the quality of the produced grape seed oil is good, and the nutrient components of the grape seed oil is maximally reserved; and particularly, the refining processes such as alkali refining, hydration, heat-cold treatment, decoloration and the like effectively removes different water-solubility and fat-soluble harmful foreign matters in the crude oil, so the quality of the grape seed oil is improved, and the quality guarantee period is prolonged.