The invention relates to the technical field of biological
fermentation, in particular to a method for synergistically fermenting distillers' grains by
bacteria and enzymes. The
viable count of the three functional
bacteria provided by the invention can reach 107CFU / g or above after the three functional
bacteria are cultured for 5 days in an environment with the temperature of 50-60 DEG C and the pH value of 3.0-4.0. In the environment, indigenous
flora naturally carried by the yellow
wine lees are difficult to survive, interference of
indigenous bacteria can be basically eliminated in the
fermentation process, growth and
reproduction of functional bacteria are facilitated, and the stability of large-scale
fermentation can be improved. The bacterial
enzyme synergistic process activates the metabolic diversity of microorganisms through enzymolysis, the content of propionic acid and
acetic acid is remarkably increased, meanwhile,
succinic acid is kept stable, and the
antibacterial activity and
metabolic balance of an
organic acid spectrum can be optimized. Meanwhile, enzymolysis can promote degradation of medium-chain
fatty acid, and enzymolysis continuously optimizes a
lipid metabolism structure in the later stage. The
proline content of a bacterium-
enzyme synergistic fermentation group is far higher than that of a pure fermentation group, so that the
palatability of the feed is remarkably improved. Moreover,
methionine is kept stable in a bacterium-
enzyme synergistic group, so that
nutrition loss can be avoided.