The invention discloses a method for extracting
pectic polysaccharide rich in RG-I based on a water-containing
deep eutectic solvent containing
cellulase, which comprises the following steps: S1, mixing purified
plant cell walls with the
deep eutectic solvent containing
cellulase, stirring and heating to obtain a
solid-liquid mixture; s2, centrifuging the
solid-liquid mixture to obtain a first supernatant and
solid residues; s3, adding water into the solid residues again, centrifuging after heating and oscillating, collecting a supernatant water solution, and repeating for five times; and S4, combining the supernatant water solutions, adding alcohols, centrifuging, collecting precipitates, and carrying out decolorization treatment. The
choline chloride /
propylene glycol deep eutectic solvent and the
cellulase are adopted to form a synergistic extraction
system, the cellulase precisely degrades a
cellulose skeleton,
choline chloride /
propylene glycol and exposed RG-I fully interact, selective release and enrichment of the RG-I structural domain are realized, the conditions are mild, high yield and low
solvent residue are ensured, the process is environment-friendly, and the method is suitable for industrial production. The
pectic polysaccharide product with high RG-I content can be efficiently prepared, and the method has industrial application potential.