32 results about "Pectic polysaccharide" patented technology

The invention discloses hawthorn leaf pectic polysaccharide with lipid-lowering activity and a preparation method and application thereof, and belongs to a preparation method of plant polysaccharide, the preparation method comprises the following steps: refluxing hawthorn leaves with ethanol, discarding monosaccharide and small molecule compounds, refluxing with hot water to extract medicinal materials, removing protein through an enzymolysis method and a Sevage reagent method, decolorizing with polyamide resin, and drying to obtain the hawthorn leaf pectic polysaccharide with lipid-lowering activity. The crude polysaccharide is separated through DEAE-52 column chromatography, and finally, the crude polysaccharide is further purified through Sephadex G-100, so that the uniform purified polysaccharide is obtained. Tests on pancreatic lipase inhibition, taurocholate binding capacity and glycocholate binding rate show that the compound has remarkable lipid-lowering activity and is expected to become a potential natural lipid-lowering preparation.

The invention provides a radix asparagi homogeneous acidic polysaccharide. The radix asparagi homogeneous acidic polysaccharide contains pectic polysaccharide which is shown as a formula 1, takes rhamnogalacturonidan I (RG-I) as a core and has a homopolygalacturonic acid (HG) structural domain. The invention further provides a preparation method and application of the radix asparagi homogeneous acidic polysaccharide. The asparagi pectin polysaccharide with a new structure is found for the first time, and the asparagi pectin polysaccharide has the effect on acute lung injury. The acidic polysaccharide disclosed by the invention has the advantage of anti-ALI activity, realizes synergistic protection of intestine-lung double target regions, has anti-inflammatory and anti-oxidation double activities, is natural in source and high in biological safety, and is suitable for long-term adjuvant therapy of acute lung injury.

The present application relates to the field of biotechnology, in particular to a kind of complex probiotic bacteria capable of synergistic metabolism pectin polysaccharide and application thereof, the complex bacterial agent includes plant lactobacillus FMBL L23180CLL and rhamnose lactobacillus FMBL L23121YWT, preservation number is CCTCC NO:M 20241793 and CCTCC NO:M 20241794 respectively;Can be realized in the medium of pectin as carbon source only rapid proliferation, high-efficiency metabolism pectin polysaccharide, promote the growth of strain, biomass is significantly improved, and probiotic index is also significantly improved;In the process of co-culture, a large amount of short-chain fatty acid is produced, and the content of acetic acid and valeric acid produced at the end of fermentation is the highest, more than 90% of total fermentation product, can be used for preparing microcapsule, complex probiotic bacterial agent, fermented milk, fruit and vegetable beverage, plant milk, probiotic milk tablet and the like, and has wide application prospect.

The invention relates to the technical field of smelting waste residues, in particular to a method for recovering nonferrous metals from smelting waste residues. The method comprises the following steps that S1, the smelting waste residues are pretreated, specifically, the smelting waste residues are copper smelting residues, and the smelting waste residues are pretreated to obtain mineral powder; and S2, a collecting agent is prepared, wherein the collecting agent is prepared from orange peel, warm water, ethyl alcohol, oleoyl chloride, acetone, triethylamine and fatty acid methyl ester. In the recovery treatment process of the smelting waste residues, by adjusting and optimizing the formula of the collecting agent and utilizing the surface activity capacity of pectin, polysaccharide and terpene contained in the orange peel, the collecting agent is endowed with good mineral affinity capacity, and the collecting agent can be used for collecting metal ions. The collecting agent can play a role in wetting and dispersing in a flotation environment, so that the collecting agent can be firmly adsorbed on the surface of copper minerals generated after melting, and the efficiency of flotation adsorption of the copper minerals is effectively improved.

The invention belongs to the technical field of cellulose resource utilization, and discloses pectic polysaccharide CSP-A2 with a remarkable enzyme protection function and application of the pectic polysaccharide CSP-A2 in increasing the yield of cellulase reducing sugar. Aiming at the problems of high production cost, reaction liquid viscosity increase, enzyme active site shielding and environmental risk caused by a chemical surfactant in a cellulose enzymolysis process, the pectic polysaccharide CSP-A2 with a remarkable enzyme protection function is prepared, and the enzyme activity of the pectic polysaccharide CSP-A2 is improved by enhancing the dispersity of enzyme molecules and promoting enzyme-substrate adsorption. The reducing sugar yield of the low-concentration cellulase under shear force and gas-liquid interface conditions is remarkably improved, and the enzymolysis efficiency is remarkably improved through the synergistic effect of the low-concentration cellulase and Tween20.

The invention relates to the technical field of beverage preparation, in particular to a refreshing and anti-fatigue rhizoma polygonati and coffee compound beverage and a preparation technology thereof. The preparation process comprises the following steps: preparing polygonatum polysaccharide; preparing gardenia pectic polysaccharide; preparing rhizoma polygonati extract gel microspheres; the refreshing and anti-fatigue rhizoma polygonati and coffee compound beverage is prepared. According to the invention, the gardenia pectic polysaccharides and the lecithin are combined and then used for coating the polygonatum saponin, and after the gardenia pectic polysaccharides and the lecithin are compounded, the substances coated in the gel can be effectively prevented from being gathered, precipitated or separated, the coated active substances can be better protected, and the influence of external environmental factors on the chemical properties of the gel is reduced; the sealwort coffee is kept in a stable state in the storage and transportation processes, and the taste enjoying period and the quality guarantee period of the sealwort coffee are prolonged.

The present application belongs to the technical field of plant extraction and separation, and discloses a method for electrophoretic migration enrichment and separation of pectin and other plant polysaccharides. The electrophoretic separation device is divided into multiple compartments, and the crude pectin extract is filtered and then put into the electrophoretic separation device. After the solution is adjusted to a suitable temperature and pH, the pectin / polysaccharide, which is electronegative, starts to migrate from the cathode to the anode under the action of the electric field. The solid content of the pectin / polysaccharide extract can be increased by 10-30 times, which greatly saves the amount of alcohol used for alcohol precipitation, significantly shortens the pectin deesterification time, and increases the deesterification rate by three times compared with the traditional deesterification method, with a recovery rate of more than 80%. The product has good emulsifying and gelling properties. Compared with the existing pectin or polysaccharide extraction and separation technology, the method is simple in process, high in separation efficiency, energy-saving, environmentally friendly and safe, can reduce the production cost of pectin and polysaccharide, and can further broaden the application of polysaccharide in the fields of food, medicine and chemical industry.

The present disclosure provides a preparation method of a pectic polysaccharide capable of regulating and controlling ice crystal growth, comprising step 1, extracting a pectin crude extract from apple powder; step 2, carrying out branched chain enzymolysis on pectin crude extract to obtain pectin enzymatic hydrolysate; step 3, adding pectin methylesterase and pectin acetylesterase into pectin enzymatic hydrolysate for precise de-esterification enzymolysis; step 4, adding 0.5-2 U / mL of polygalacturonase into pectin enzymatic hydrolysate obtained in step 3, and hydrolyzing for 1.5-2.5 hours; and step 5, collecting precipitate in pectin enzymatic hydrolysate obtained in step 4 to obtain pectic polysaccharide. The pectic polysaccharide has a purity higher than 90%, a methyl esterification degree of 45-55%, an acetylation degree less than 2%, a neutral sugar content less than 5%, and an average molecular weight of 1.4-7.5 kDa, and obviously reduces an ice crystal size in a freezing process.

The invention discloses a preparation method of fructus alpiniae oxyphyllae pectic polysaccharide and application of the fructus alpiniae oxyphyllae pectic polysaccharide in treatment of Alzheimer's disease. The pectic polysaccharide is prepared by taking fructus alpiniae oxyphyllae as a raw material through the steps of degreasing, water extraction, alcohol precipitation, deproteinization, dialysis, chromatographic purification and the like, and is a uniform acidic polysaccharide. Structural characterization results show that the pectic polysaccharide is mainly composed of rhamnose, glucuronic acid, galacturonic acid and galactose, the content of galacturonic acid is dominant, part of residues are subjected to O-methylation and / or O-acetylation modification, and the pectic polysaccharide has a pectic acidic polysaccharide structure with alpha-(1-> 4)-D-galacturonic acid as a main chain and branched chains. Animal experiment results show that the fructus alpiniae oxyphyllae pectic polysaccharide can significantly improve the learning and memory ability and cognitive impairment of Alzheimer disease model animals. The fructus alpiniae oxyphyllae pectic polysaccharide provided by the invention is clear in source and stable in preparation method, can be used for preparing a pharmaceutical composition or functional food for preventing and treating the Alzheimer disease, and has a good application prospect.

The invention discloses application of common turnip polysaccharide in preparation of a product for preventing alcoholic liver injury and assisting in dispelling the effects of alcohol, and belongs to the technical field of natural product development and biological medicine. The common turnip polysaccharide prepared through ultrasonic and alcohol extraction is rich in galactose and rhamnose, has high galacturonic acid content, shows typical RG-I type pectic polysaccharide characteristics, can be kept stable in simulated gastrointestinal fluid and directly reaches the colon to play a role; meanwhile, the prepared common turnip polysaccharide can remarkably reduce transaminase increase and lipid accumulation caused by alcohol, and by specifically proliferating beneficial bacteria such as Akkermansia and the like, the intestinal barrier is repaired, and endotoxin is prevented from entering blood; meanwhile, liver hangover alleviating enzymes can be obviously activated, and the drunkenness time is shortened.

The invention discloses clove dreg polysaccharide with immune regulation and intestinal flora regulation functions and a preparation method thereof. The preparation method comprises the steps that dried medicine residues obtained after clove is subjected to oil extraction are taken and smashed, then water extraction, concentration, alcohol precipitation, deproteinization and other steps are carried out to obtain clove medicine residue polysaccharide, the novel clove heteropolysaccharide is further obtained through anion exchange chromatography purification, and the heteropolysaccharide is I-type rhamnogalacturonic acid pectic polysaccharide, is composed of eight monosaccharides and has specific structural characteristics. Experiments prove that the polysaccharide prepared by the invention can obviously improve the levels of serum IL-2, IL-6, IFN-gamma, IgG, IgA and IgM, enhance the immunity of the organism, regulate the diversity of intestinal flora, promote the proliferation of beneficial bacteria such as Brautella and clostridium, and inhibit harmful bacteria such as tremospirillum. According to the invention, the resource utilization of the clove dregs is realized, and a new way is provided for developing immunoregulation and intestinal health products.

Spherical cholesteric liquid crystal shells may be produced by mixing boron nitride nanotubes (BNNTs) with cellulose nanocrystals (CNCs) in water. The BNNTs may be coated with a polysaccharide (e.g., pectin). The polysaccharide may function as a dispersing agent and an emulsion stabilizer. Associated compositions, methods, films, and articles employ the shells. The shells may form spherical droplets with isotropic cores.

The present invention relates to the technical field of natural product preparation, and specifically to a D-galacturonic acid polysaccharide homogeneous pectin polysaccharide and its preparation method and application. The monosaccharide composition of the polysaccharide includes galacturonic acid, galactose, rhamnose, glucuronic acid and glucose; its skeleton structure is composed of →4)-α-GalpA-(1→ and →4)-α-GalpA-6-O-methyl-(1→. The polysaccharide can be obtained by lactic acid bacteria fermentation and extraction of Liangping pomelo and purification by column chromatography. The preparation method is simple, safe and suitable for expanded production. The polysaccharide has α-glucosidase inhibitory activity, and the α-glucosidase inhibition rate can reach 80%. IC 50 The value can reach 0.128 mg / mL, which lays a foundation for further research on α-glucosidase inhibitors and drugs for treating diabetes. It has broad promotion and application value, and at the same time creates conditions for the comprehensive utilization of Liangping pomelo resources.

The invention belongs to the technical field of traditional Chinese medicine active polysaccharide and preparation thereof, and particularly relates to gardenia pectic polysaccharide as well as a preparation method and application thereof. The gardenia pectic polysaccharide is composed of rhamnose, galactose, glucose and galacturonic acid. The molar ratio of the rhamnose to the galactose to the glucose to the galacturonic acid is 1: 1.07: 1.52: 2.00. The preparation method provided by the invention is simple in process, green and environment-friendly, and relatively high in polysaccharide purity and yield. The pectic polysaccharide prepared by the invention has the effect of promoting and regulating the proliferation of intestinal flora, particularly obviously promotes the growth of intestinal acid-producing and gas-producing bacteria, and has a wide application prospect in the fields of functional foods and health-care products.

The present invention relates to the technical field of extracting polysaccharides from Prunella vulgaris, and discloses a polysaccharide from Prunella vulgaris, an extraction method thereof, and an application thereof. The method comprises: (1) crushing and sieving Prunella vulgaris fruit spikes, and performing a defatting treatment with an organic solvent; (2) mixing the defatted Prunella vulgaris powder with water, stirring at 20-28°C, and repeating the process 2-4 times to obtain a first medicinal material residue; (3) stirring the first medicinal material residue in hot water at 75-85°C, and repeating the process 1-3 times; and stirring the obtained second medicinal material residue with acidified water; (4) extracting the third medicinal material residue with an ammonium oxalate solution, and repeating the extraction process 1-3 times; and decolorizing and deproteinizing the collected extract. The present invention sequentially pre-treats Prunella vulgaris with cold water, hot water, and acidified water, and then extracts the polysaccharide with an ammonium oxalate solution to obtain a polysaccharide from Prunella vulgaris. The polysaccharide has high purity and yield, and is identified as a pectin polysaccharide. The pectin polysaccharide from Prunella vulgaris has a good preventive and therapeutic effect on alcoholic liver injury.

The invention relates to the technical field of biology, in particular to bacteroides fragilis FMBL Ba24006 DMX for metabolism of pectic polysaccharides and application, the bacteroides fragilis FMBL Ba24006 DMX is preserved in China Center for Type Culture Collection on January 20, 2026, the preservation number is CCTCC M 2026175, the bacteroides fragilis FMBL Ba24006 DMX has good pectin metabolism capacity, pectin can be effectively used as a carbon source to achieve rapid proliferation, and meanwhile, the bacteroides fragilis FMBL Ba24006 DMX can be used for metabolism of pectic polysaccharides. The bacterial strain has a good antibacterial effect on hemorrhagic Escherichia coli; the compound is sensitive and moderately sensitive to cefoxitin and rifampicin. The strain can produce a large amount of short-chain fatty acid when being used for fermenting pectin, and fermentation liquor taking the pectin as a carbon source has relatively strong scavenging capacity on DPPH and ABTS free radicals; a relatively good inhibition effect is achieved on the activity of alpha-amylase; the method can be used for preparing antioxidant products, fermented foods, health-care products, food additives and feeds, and has a wide application prospect.

The invention relates to a synbiotic composition comprising Bifidobacterium ssp., non- digestible oligosaccharides, and pectin polysaccharides having a molecular weight between 50-2000 kDa, the backbone thereof comprising at least 55% by weight of the polysaccharide of 1,4-linked a-D-galacturonic acid residues and having a degree of methylation (DM) of at least 35% and / or a pectin polysaccharide that has a degree of polymerisation of at least 260. The invention further relates to a nutritional composition comprising the synbiotic composition. The invention further relates to the use of the synbiotic composition for treating or preventing gut dysbiosis, preferably antibiotic-induced gut dysbiosis.

This invention discloses a tea pectin polysaccharide and its application in immune regulation and prevention of colitis, relating to the field of biomedical technology. The invention provides a tea pectin polysaccharide whose main chain structure is composed of (1→4)-α-D-GalpA-(1→) linked together, with the repeating structural unit being (1→4)-α-D-GalpA. The (1→4)-α-D-GalpA-(1→) residues of the tea pectin polysaccharide exhibit acetylation at positions O-2 and O-3, and methyl esterification at position C-6, with a degree of acetylation of 14.80% and a degree of methyl esterification of 42.47%. The main chain structure of the pectin polysaccharide after oxidative degradation is composed of α-D-G... The structure consists of alpA-(1→4)-α-D-GalpA-(1→link and →4)-α-D-GalpA-(1→4)-β-D-GalpA links, with the linking sites located at positions O-4. The tea pectin polysaccharide of this invention and its related preparations exhibit excellent immunomodulatory effects, playing a significant role in anti-inflammatory and anti-cancer processes. It is particularly valuable in the prevention and treatment of colitis, cardiovascular diseases, immune diseases, neurodegenerative diseases, and other chronic diseases.

The invention relates to the technical field of microorganisms, in particular to a bacillus subtilis peb05 strain and application of the bacillus subtilis peb05 strain. The bacillus subtilis peb05 provided by the invention has high yield of pectinase and a certain amount of xylanase and phytase, and the produced compound enzyme has good stress resistance, salt resistance, acid resistance and certain high temperature resistance, and has efficient degradation activity on pectic polysaccharide. The strain has a synergistic fermentation effect in a soy sauce making process, and can reduce the turbidity of crude oil, improve the levels of amino acid nitrogen and reducing sugar, improve the utilization rate of raw materials and improve the quality of soy sauce; the prepared compound enzyme preparation can improve the clarity of a soy sauce product in the shelf life, reduce secondary precipitation and improve the quality of the soy sauce product.

The invention provides a method of producing a hydrolysed pectic polysaccharide isolate that is enriched in rhamnogalacturonan-I, said method comprising the steps of:providing a pectin-rich substrate that has been obtained from plant material without the use of organic solvent, said pectin-rich substrate containing at least 3% by weight of dry matter of pectic polysaccharides;subjecting the pectin-rich substrate to enzymatic treatment to partially hydrolyse the pectic polysaccharides, said treatment enzymatic treatment comprising the use of one or more pectinases selected from pectin lyase (EC4.2.2.10), pectate lyase (EC 4.2.2.2), rhamnogalacturonan galacturonohydrolase (EC 3.2.1.173), endo-polygalacturonase (EC 3.2.1.15), exopolygalacturonase (EC 3.2.1.67 and EC 3.2.1.82);subjecting the partially hydrolysed pectic polysaccharides to ultrafiltration using an ultrafiltration membrane having a molecular weight cut-off in the range of 5 to 100 kDa; andrecovering the ultrafiltration retentate.The present invention further relates to the hydrolysed pectic polysaccharide isolate obtained by the present method and to a process of preparing a product selected from a nutritional formulation, a food product, a dietary supplement, a beverage or a pharmaceutical product, said process comprising addition of the aforementioned hydrolysed pectic polysaccharide isolate.

This invention relates to the field of Alpinia oxyphylla pectin polysaccharide technology, specifically a method for preparing Alpinia oxyphylla pectin polysaccharide, its polysaccharide composition, and its applications. The preparation method includes the following steps: S1: Pulverize whole Alpinia oxyphylla fruit, extract using hot water reflux, then concentrate, centrifuge, and add ethanol for precipitation to obtain an alcohol precipitate; S2: Dissolve the alcohol precipitate in water, add a protein removal reagent to remove plant protein, add ethanol again for precipitation, separate the precipitate, and obtain crude Alpinia oxyphylla polysaccharide; S3: Separate the crude Alpinia oxyphylla polysaccharide using a DEAE-52 cellulose column, and use a salt gradient elution to obtain two Alpinia oxyphylla polysaccharide solutions; the whole Alpinia oxyphylla fruit includes the pericarp and kernel. This invention uses whole Alpinia oxyphylla fruit as material to separate and extract two novel polysaccharide components, which have antidepressant and intestinal flora imbalance treatment effects, and can be used to prepare antidepressant or intestinal flora imbalance treatment drugs or functional foods.

The present application relates to the field of biotechnology, in particular to a plant lactobacillus FMBL L23180CLL capable of metabolizing pectin polysaccharide, bacterial agent and application thereof, the plant lactobacillus FMBL L23180CLL is preserved in China typical culture preservation center on August 15, 2024, and the preservation number is CCTCC NO:M 20241793;The strain of the application has good metabolic ability of pectin, can effectively utilize pectin as carbon source to realize rapid proliferation, can ferment pectin to produce a large amount of short-chain fatty acids, has good carbon source utilization ability and acid and bile salt tolerance performance;It can be used for preparing fermented food, health products, food additives and feed, and has wide application prospect.

The application discloses a preparation method of a complex pectin polysaccharide applied to the field of pectin polysaccharide preparation, which uses citrus and legume peel residues as raw materials, adopts a medium-high temperature dilute acid hydrolysis technology to prepare a polysaccharide concentrated solution, uses divalent cations mainly including calcium ions as a crosslinking agent, makes part of linear structure pectin polysaccharides and globular structure pectin polysaccharides form calcium bridges through ionic bonds to produce crosslinking, uses organic carboxylate as a chelating agent, controls the calcium bridge crosslinking in a proper range through part of divalent cations in the chelating system, avoids excessive calcium crosslinking and produces insoluble pectin polysaccharides, uses alcohol as a dehydrating agent to remove the hydration film on the surface of part of pectin polysaccharides, simultaneously inhibits the dissociation of carboxyl groups on part of pectin polysaccharide molecules through hydrogen ions, promotes part of linear pectin polysaccharides and globular pectin polysaccharides to produce crosslinking under the action of hydrogen bonds, and finally forms the complex pectin polysaccharide which is mainly crosslinked by calcium bridges and supplemented by hydrogen bonds.

The invention belongs to the field of functional food materials, and provides a uric acid reducing soybean peptide material, a preparation method of the uric acid reducing soybean peptide material and application of the uric acid reducing soybean peptide material in soybean milk powder. The preparation method comprises the following steps: constructing a metal polyphenol network through lipopeptide acylation modification, liposome embedding, alternate deposition of epigallocatechin gallate and calcium ions, deposition of chitosan or chitosan oligosaccharide and low-methoxyl pectic polysaccharide protective layers, microencapsulation of sodium alginate and other technical means. The excellent performance that the half inhibitory concentration of xanthine oxidase reaches 0.6 mg / mL or below, the 2-hour accumulative release amount under the gastric acid condition is controlled within 20%, the 2-hour accumulative release amount under the intestinal environment reaches 70% or above, and the leakage rate is lower than 20% after storage for 12 weeks is achieved, the technical problems that the uric acid reducing effect of soybean peptide is unstable, controlled release is insufficient, and the gastric acid stability is poor are solved, and the market prospect is broad. And the method has wide functional food application value.

The present application relates to the field of biotechnology, and in particular to a pentose lactobacillus plantarum FMBL L24020DMX for metabolizing pectin polysaccharide and application, the pentose lactobacillus plantarum FMBL L24020DMX is preserved in China typical culture preservation center on August 26, 2024, and the preservation number is CCTCC NO:M 20241852;It has good ability to metabolize pectin, can effectively utilize pectin as carbon source to realize rapid proliferation, produce a large amount of short-chain fatty acid;At the same time, it has strong scavenging capacity to DPPH and ABTS free radicals;It has good inhibitory effect on alpha-glucosidase and alpha-amylase activity;It can be used for preparing antioxidant products, hypoglycemic drugs, fermented foods, health products, food additives and feed, and has wide application prospect.

The application discloses a targeted steady-state extraction and concentration method of berry anthocyanin, and comprises the following steps: raw material pretreatment, targeted steady-state extraction, adding food-grade green extraction solvent and composite color protection antioxidant into the fruit pulp, adding a composite enzyme preparation composed of pectinase and cellulase, and adopting ultrasonic assisted enzyme hydrolysis to cooperatively assist extraction; centrifugal separation is conducted on the extraction liquid, and then microfiltration membrane filtration is conducted to remove pectin and polysaccharide macromolecular impurities; vacuum low-temperature concentration and nanofiltration membrane concentration coupling process are adopted for concentration, and natural food-grade stabilizers are added during the whole concentration process, so that the berry anthocyanin concentrated solution is prepared; the extraction mode of ultrasonic assisted enzyme hydrolysis under normal pressure is adopted, and expensive special equipment such as high pressure and ultrahigh pressure is not needed; the anthocyanin is realized directional dissolution by combining physical wall breaking and biological enzyme hydrolysis; the extraction efficiency is much higher than that of traditional extraction process; and the equipment investment and operation cost are greatly reduced.

The invention relates to a method of producing high and low molecular weight pectic saccharides that are enriched in HG and RG-I, said method comprising the steps providing potato pulp as pectin-rich substrate, subjecting the pectin-rich substrate to enzymatic treatment, said enzymatic treatment comprising the use of one pectinase having an endo-polygalacturonase (EC 3.2.1.15) activity, subjecting the hydrolyzed pectic polysaccharides to microfiltration using microfiltration membranes having a molecular cut-off of 0.45 and 0.8 µm, recovering the microfiltration retentate, subjecting the microfiltration permeate to nanofiltration using a membrane having a molecular weight cut-off in the range of 150 – 300 Da, recovering the nanofiltration retentate.

The present application relates to the field of microbial technology, and particularly to a bacillus subtilis (Bacillus subtilis) peb05 and application thereof. Bacillus subtilis The bacillus subtilis peb05 provided in the present application has high pectinase yield, and a certain amount of xylanase and phytase, and the produced compound enzyme has good stress resistance, salt resistance, acid resistance and certain high-temperature resistance, and has high efficient degradation activity on pectin polysaccharide. The strain has synergistic fermentation effect in the process of soy sauce production, can reduce the turbidity of raw oil, improve the levels of amino acid nitrogen and reducing sugar, improve the utilization rate of raw materials, and improve the quality of soy sauce; the prepared compound enzyme preparation can improve the clarity of soy sauce product during the shelf period, reduce the secondary precipitation, and improve the quality of soy sauce product.

The invention discloses a traditional Chinese medicine polysaccharide dressing for diabetic wound healing and a preparation method thereof. The traditional Chinese medicine polysaccharide dressing is formed by compounding a pectic polysaccharide / polymer electrospun nanofiber layer and a latticed hydrogel matrix in which traditional Chinese medicine extracts such as cortex phellodendri, fructus gardeniae and naringin are embedded, the fiber layer rapidly absorbs seepage and keeps moist, the gel layer achieves continuous slow release and structural support through auxiliaries such as sodium hyaluronate and the like, an Mg-Zn nano system is assisted to achieve synergistic antibiosis and cell proliferation promotion, the thickness of the dressing is 0.3-1.2 mm, the dressing can be made into a pasting film or a patch, and the dressing has the functions of high liquid absorption rate, oxygen release, bacteriostasis and regeneration promotion. The healing of chronic wound surfaces of diabetes mellitus can be accelerated.

The invention discloses a preparation method of elsholtzia ciliata pectin with an immunomodulatory effect. The preparation method comprises the following steps: step 1, raw material pretreatment, step 2, oriented extraction of pectin, step 3, preliminary purification, step 4, column chromatography separation, step 5, refining purification, step 6, physicochemical property detection, step 7, structural characterization, and step 8, immunomodulatory activity verification. The preparation method of the wild celery pectin is clear in technological parameters, stable in operation and high in repeatability, the pectic polysaccharide WOSP-0.3 with high purity and good uniformity is successfully separated and purified from wild celery through the steps of directional extraction, column chromatography separation, refining purification and the like, the yield reaches up to 53.29%, and the utilization rate of wild celery resources is remarkably increased.