The invention discloses an enzymatic extraction method for
auricularia auricula polysaccharides, which comprises the following steps of: mechanically and coarsely
grinding dried
auricularia auricula, sieving, mixing according to a material-to-water ratio of 1:(50-200), and extracting at the temperature of between 35 and 50 DEG C and the pH of between 5.0 and 9.0 under the action of
chitinase for 60 to 180 minutes, wherein the addition amount of the
chitinase is 100 to 600U / g; raising the extraction temperature to be between 80 and 95 DEG C, extracting for 60 to 180 minutes to obtain extract, and centrifuging to obtain supernatant and precipitates; and adding
ethanol into the supernatant to precipitate polysaccharides, performing
centrifugal separation to obtain precipitates, dissolving the precipitates in water, concentrating to remove residual
ethanol, adding a mixture of diatomite and
activated carbon into concentrated solution, mixing, fully stirring, filtering, performing membrane
filtration on filtrate by using a
polysulfone membrane of 30 to 100kD, collecting macromolecular concentrated solution which does not filter through the membrane, and
drying the macromolecular concentrated solution to obtain the
auricularia auricula polysaccharides. The prepared products have high purity and bioactivity; and the
polysaccharide content is over 40 percent.