Enzymatic extraction method for auricularia auricula polysaccharides
A black fungus polysaccharide and enzymatic extraction technology, applied in the directions of antitoxin, digestive system, metabolic diseases, etc., can solve the problems of reducing bioavailability, easily destroying polysaccharide structure, etc., achieving stable and fast ultrafiltration speed, shortening process time, Ensuring the effect of ultrafiltration speed
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Embodiment 1
[0031] 1 Materials and reagents
[0032] Black fungus (provided by Zhejiang Fulaisen Green Industry Group Co., Ltd.); neutral protease, pectinase, chitinase, 3,5-dinitrosalicylic acid (DNS), absolute ethanol, concentrated sulfuric acid, glucose , phenol, were analytically pure.
[0033] 2 experimental equipment
[0034] UV-2100 UV-Vis Spectrophotometer, Mettler Tolefo 320-S pH Meter, TOMY ES-315 Autoclave, ZHWY-2102C Constant Temperature Shaker Incubator, Precision Booster Electric Stirrer JJ-1, HH-2 Digital Display Constant temperature water bath, DL-5M desktop high-speed centrifuge, DHG-9240A electric constant temperature blast drying oven, EL204 electronic balance.
[0035] 3 Experimental methods
[0036] 3.1 Technical route of polysaccharide extraction
[0037] Crush the black fungus, pass through a 60-mesh sieve → use a material-to-water ratio of 1:100, at a pH of 8.0, add 400 U / g of enzyme, extract at 45°C for 1.65h → increase the temperature to 85°C for 1.5h → filte...
Embodiment 2
[0116] The experimental operation is the same as in Example 1, except that in step 3.1, the black fungus is crushed, passed through a 60-mesh sieve, and 20 g is taken at a material-to-water ratio of 1:98. At a pH value of 8.0, 200 U / g of chitinase is added. After extracting for 99 minutes at a temperature of 42° C., the temperature was raised to 85° C. and extracted for 90 minutes. The subsequent steps were all the same as in Example 1 to obtain 2.57 g of black fungus polysaccharides, the polysaccharide content was 41.26%, and the dissolution rate of black fungus polysaccharides was 12.85%.
Embodiment 3
[0118] The experimental operation is the same as in Example 1, except that in step 3.1, the black fungus is crushed, passed through a 60-mesh sieve, and 20 g is taken at a material-to-water ratio of 1:98. At a pH value of 8.0, 600 U / g of chitinase is added. After extracting for 99 minutes at a temperature of 42° C., the temperature was raised to 85° C. and extracted for 90 minutes. The subsequent steps were all the same as in Example 1 to obtain 2.974 g of black fungus polysaccharides, the polysaccharide content was 44.61%, and the dissolved amount of black fungus polysaccharides was 14.87%.
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