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Enzymatic extraction method for auricularia auricula polysaccharides

A black fungus polysaccharide and enzymatic extraction technology, applied in the directions of antitoxin, digestive system, metabolic diseases, etc., can solve the problems of reducing bioavailability, easily destroying polysaccharide structure, etc., achieving stable and fast ultrafiltration speed, shortening process time, Ensuring the effect of ultrafiltration speed

Inactive Publication Date: 2011-05-18
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional black fungus polysaccharide extraction uses water extraction and alcohol precipitation, and it is difficult for the polysaccharide to diffuse out of the cell; while the acid and alkali methods easily destroy the structure of the polysaccharide and reduce its bioavailability

Method used

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  • Enzymatic extraction method for auricularia auricula polysaccharides
  • Enzymatic extraction method for auricularia auricula polysaccharides
  • Enzymatic extraction method for auricularia auricula polysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1 Materials and reagents

[0032] Black fungus (provided by Zhejiang Fulaisen Green Industry Group Co., Ltd.); neutral protease, pectinase, chitinase, 3,5-dinitrosalicylic acid (DNS), absolute ethanol, concentrated sulfuric acid, glucose , phenol, were analytically pure.

[0033] 2 experimental equipment

[0034] UV-2100 UV-Vis Spectrophotometer, Mettler Tolefo 320-S pH Meter, TOMY ES-315 Autoclave, ZHWY-2102C Constant Temperature Shaker Incubator, Precision Booster Electric Stirrer JJ-1, HH-2 Digital Display Constant temperature water bath, DL-5M desktop high-speed centrifuge, DHG-9240A electric constant temperature blast drying oven, EL204 electronic balance.

[0035] 3 Experimental methods

[0036] 3.1 Technical route of polysaccharide extraction

[0037] Crush the black fungus, pass through a 60-mesh sieve → use a material-to-water ratio of 1:100, at a pH of 8.0, add 400 U / g of enzyme, extract at 45°C for 1.65h → increase the temperature to 85°C for 1.5h → filte...

Embodiment 2

[0116] The experimental operation is the same as in Example 1, except that in step 3.1, the black fungus is crushed, passed through a 60-mesh sieve, and 20 g is taken at a material-to-water ratio of 1:98. At a pH value of 8.0, 200 U / g of chitinase is added. After extracting for 99 minutes at a temperature of 42° C., the temperature was raised to 85° C. and extracted for 90 minutes. The subsequent steps were all the same as in Example 1 to obtain 2.57 g of black fungus polysaccharides, the polysaccharide content was 41.26%, and the dissolution rate of black fungus polysaccharides was 12.85%.

Embodiment 3

[0118] The experimental operation is the same as in Example 1, except that in step 3.1, the black fungus is crushed, passed through a 60-mesh sieve, and 20 g is taken at a material-to-water ratio of 1:98. At a pH value of 8.0, 600 U / g of chitinase is added. After extracting for 99 minutes at a temperature of 42° C., the temperature was raised to 85° C. and extracted for 90 minutes. The subsequent steps were all the same as in Example 1 to obtain 2.974 g of black fungus polysaccharides, the polysaccharide content was 44.61%, and the dissolved amount of black fungus polysaccharides was 14.87%.

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Abstract

The invention discloses an enzymatic extraction method for auricularia auricula polysaccharides, which comprises the following steps of: mechanically and coarsely grinding dried auricularia auricula, sieving, mixing according to a material-to-water ratio of 1:(50-200), and extracting at the temperature of between 35 and 50 DEG C and the pH of between 5.0 and 9.0 under the action of chitinase for 60 to 180 minutes, wherein the addition amount of the chitinase is 100 to 600U / g; raising the extraction temperature to be between 80 and 95 DEG C, extracting for 60 to 180 minutes to obtain extract, and centrifuging to obtain supernatant and precipitates; and adding ethanol into the supernatant to precipitate polysaccharides, performing centrifugal separation to obtain precipitates, dissolving the precipitates in water, concentrating to remove residual ethanol, adding a mixture of diatomite and activated carbon into concentrated solution, mixing, fully stirring, filtering, performing membrane filtration on filtrate by using a polysulfone membrane of 30 to 100kD, collecting macromolecular concentrated solution which does not filter through the membrane, and drying the macromolecular concentrated solution to obtain the auricularia auricula polysaccharides. The prepared products have high purity and bioactivity; and the polysaccharide content is over 40 percent.

Description

(1) Technical field [0001] The invention relates to a method for preparing black fungus polysaccharide by enzymatic method. (2) Background technology [0002] Black fungus Auricularia auricula (L.exHook.) Underwood, also known as light fungus, fine fungus, cloud ear, is a large fungus, belonging to Basidiomycetes, Auricularia, Agaricaceae, Auricularia. A large number of medical and pharmacological studies have shown that black fungus polysaccharides have anti-platelet aggregation, anti-tumor, anti-aging, antibacterial, hypoglycemic, immune function regulation, anti-ulcer, anti-hepatitis and anti-mutation, hypolipidemic and promote serum protein biosynthesis and other functions, and proposed that black fungus has the functions of moistening the lungs, clearing the stomach and intestines, losing weight, and facilitating the timely removal and discharge of toxic substances in the body, all of which are related to its rich non-starch polysaccharides. Such as Li Wenning (2003) i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P1/04A61P1/16A61P3/06A61P3/10A61P7/02A61P31/04A61P35/00A61P37/04A61P39/00
Inventor 张拥军鲜乔何杰民李佳朱丽云
Owner CHINA JILIANG UNIV
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